longtext: 1D07-pdb

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HEADER    HYDROLASE                               09-SEP-99   1D07
TITLE     HYDROLYTIC HALOALKANE DEHALOGENASE LINB FROM SPHINGOMONAS
TITLE    2 PAUCIMOBILIS UT26 WITH 1,3-PROPANEDIOL, A PRODUCT OF
TITLE    3 DEBROMIDATION OF DIBROMPROPANE, AT 2.0A RESOLUTION
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: HALOALKANE DEHALOGENASE;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: LINB, 1,3,4,6-TETRACHLORO-1,4-CYCLOHEXADIENE
COMPND   5 HYDROLASE;
COMPND   6 EC: 3.8.1.5;
COMPND   7 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: SPHINGOMONAS PAUCIMOBILIS;
SOURCE   3 STRAIN: UT26;
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   5 EXPRESSION_SYSTEM_STRAIN: HB101;
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: PMYLB1
KEYWDS    DEHALOGENASE, LINDANE, BIODEGRADATION, ALPHA/BETA-HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    J.MAREK,J.VEVODOVA,J.DAMBORSKY,I.SMATANOVA,L.A.SVENSSON,
AUTHOR   2 J.NEWMAN,Y.NAGATA,M.TAKAGI
REVDAT   1   11-SEP-00 1D07    0
JRNL        AUTH   J.MAREK,J.VEVODOVA,I.SMATANOVA,L.A.SVENSSON,
JRNL        AUTH 2 J.NEWMAN,Y.NAGATA,J.DAMBORSKY,M.TAKAGI
JRNL        TITL   CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE LINB
JRNL        TITL 2 FROM SPHINGOMONAS PAUCIMOBILIS UT26 AT 1.6 A
JRNL        TITL 3 RESOLUTION
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   1
REMARK   1 REFERENCE 1
REMARK   1  AUTH   I.SMATANOVA,Y.NAGATA,L.A.SVENSSON,M.TAKAGI,J.MAREK
REMARK   1  TITL   CRYSTALLIZATION AND PRELIMINARY X-RAY DIFFRACTION
REMARK   1  TITL 2 ANALYSIS OF HALOALKANE DEHALOGENASE LINB FROM
REMARK   1  TITL 3 SPHINGOMONAS PAUCIMOBILIS UT26
REMARK   1  REF    ACTA CRYSTALLOGR., SECT.D     V.  53  1231 1999
REMARK   1  REFN   ASTM ABCRE6  DK ISSN 0907-4449
REMARK   2
REMARK   2 RESOLUTION. 2.00 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : SHELXL-96
REMARK   3   AUTHORS     : G.M.SHELDRICK
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.00
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 20.0
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 86.6
REMARK   3   CROSS-VALIDATION METHOD           : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION   : RANDOM
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (NO CUTOFF).
REMARK   3   R VALUE   (WORKING + TEST SET, NO CUTOFF) : 0.180
REMARK   3   R VALUE          (WORKING SET, NO CUTOFF) : 0.169
REMARK   3   FREE R VALUE                  (NO CUTOFF) : 0.274
REMARK   3   FREE R VALUE TEST SET SIZE (%, NO CUTOFF) : 8.000
REMARK   3   FREE R VALUE TEST SET COUNT   (NO CUTOFF) : 1385
REMARK   3   TOTAL NUMBER OF REFLECTIONS   (NO CUTOFF) : 16801
REMARK   3
REMARK   3  FIT/AGREEMENT OF MODEL FOR DATA WITH F>4SIG(F).
REMARK   3   R VALUE   (WORKING + TEST SET, F>4SIG(F)) : 0.170
REMARK   3   R VALUE          (WORKING SET, F>4SIG(F)) : 0.159
REMARK   3   FREE R VALUE                  (F>4SIG(F)) : 0.258
REMARK   3   FREE R VALUE TEST SET SIZE (%, F>4SIG(F)) : 8.000
REMARK   3   FREE R VALUE TEST SET COUNT   (F>4SIG(F)) : 1124
REMARK   3   TOTAL NUMBER OF REFLECTIONS   (F>4SIG(F)) : 13620
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS      : 2301
REMARK   3   NUCLEIC ACID ATOMS : 0
REMARK   3   HETEROGEN ATOMS    : 2
REMARK   3   SOLVENT ATOMS      : 274
REMARK   3
REMARK   3  MODEL REFINEMENT.
REMARK   3   OCCUPANCY SUM OF NON-HYDROGEN ATOMS      : 2574.00
REMARK   3   OCCUPANCY SUM OF HYDROGEN ATOMS          : 0.00
REMARK   3   NUMBER OF DISCRETELY DISORDERED RESIDUES : NULL
REMARK   3   NUMBER OF LEAST-SQUARES PARAMETERS       : 1036
REMARK   3   NUMBER OF RESTRAINTS                     : 971
REMARK   3
REMARK   3  RMS DEVIATIONS FROM RESTRAINT TARGET VALUES.
REMARK   3   BOND LENGTHS                         (A) : 0.005
REMARK   3   ANGLE DISTANCES                      (A) : 0.021
REMARK   3   SIMILAR DISTANCES (NO TARGET VALUES) (A) : NULL
REMARK   3   DISTANCES FROM RESTRAINT PLANES      (A) : 0.024
REMARK   3   ZERO CHIRAL VOLUMES               (A**3) : 0.025
REMARK   3   NON-ZERO CHIRAL VOLUMES           (A**3) : 0.035
REMARK   3   ANTI-BUMPING DISTANCE RESTRAINTS     (A) : 0.020
REMARK   3   RIGID-BOND ADP COMPONENTS         (A**2) : NULL
REMARK   3   SIMILAR ADP COMPONENTS            (A**2) : 0.079
REMARK   3   APPROXIMATELY ISOTROPIC ADPS      (A**2) : NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELING.
REMARK   3   METHOD USED: MOEWS & KRETSINGER: J.MOL.BIOL. 91 (1973)
REMARK   3                201-228
REMARK   3
REMARK   3  STEREOCHEMISTRY TARGET VALUES : NULL
REMARK   3   SPECIAL CASE: NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 1D07 COMPLIES WITH FORMAT V. 2.3, 09-JULY-1998
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 09-SEP-1999.
REMARK 100 THE RCSB ID CODE IS RCSB009670.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 04-DEC-1998
REMARK 200  TEMPERATURE           (KELVIN) : 100.0
REMARK 200  PH                             : 8.90
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : PHOTON FACTORY
REMARK 200  BEAMLINE                       : BL 711
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9000
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MAR
REMARK 200  DATA SCALING SOFTWARE          : XDS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 16801
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.000
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 86.6
REMARK 200  DATA REDUNDANCY                : 7.670
REMARK 200  R MERGE                    (I) : 0.11300
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 12.3700
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.13
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.8
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.64
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 4.550
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR
REMARK 200  REPLACEMENT
REMARK 200 SOFTWARE USED: AMORE
REMARK 200 STARTING MODEL: 1CV2
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 40.2
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.07
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 19% (W/V) PEG 6000, 100 MM
REMARK 280  TRIS-HCL, 200MM CA ACETATE, PH=8.9. CRYSTAL WAS SOAKED 4
REMARK 280  HOURS IN RESERVOIR LIQUOR WITH 25MM 1,3-DIBROMPROPANE AT
REMARK 280  278 K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z
REMARK 290       3555   1/2-X,1/2+Y,-Z
REMARK 290       4555   1/2+X,1/2-Y,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       25.11350
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       35.86050
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       25.11350
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       35.86050
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT
REMARK 300 WHICH CONSISTS OF 1 CHAIN(S). SEE REMARK 350 FOR
REMARK 300 INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S).
REMARK 350
REMARK 350 GENERATING THE BIOMOLECULE
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 400
REMARK 400 COMPOUND
REMARK 400 LINB IS A MEMBER OF ALPHA/BETA HYDROLASE FAMILY.
REMARK 400
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     SER A     2
REMARK 465     LEU A     3
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS(M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     GLU A  10    CB    CG    CD    OE1   OE2
REMARK 470     ASP A  73    CG    OD1   OD2
REMARK 470     GLU A 145    CG    CD    OE1   OE2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    LEU A  44   CA  -  CB  -  CG  ANGL. DEV. = 12.6 DEGREES
REMARK 500    ALA A 296   C   -  N   -  CA  ANGL. DEV. = 16.2 DEGREES
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASP A 108       48.08   -116.39
REMARK 525
REMARK 525 SOLVENT
REMARK 525 THE FOLLOWING SOLVENT MOLECULES LIE FARTHER THAN EXPECTED
REMARK 525 FROM THE PROTEIN OR NUCLEIC ACID MOLECULE AND MAY BE
REMARK 525 ASSOCIATED WITH A SYMMETRY RELATED MOLECULE (M=MODEL
REMARK 525 NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525  0 HOH  1114        DISTANCE =  5.19 ANGSTROMS
REMARK 525  0 HOH  1154        DISTANCE =  5.45 ANGSTROMS
REMARK 525  0 HOH  1156        DISTANCE =  5.70 ANGSTROMS
REMARK 525  0 HOH  1168        DISTANCE =  6.25 ANGSTROMS
REMARK 525  0 HOH  1177        DISTANCE =  5.17 ANGSTROMS
REMARK 525  0 HOH  1181        DISTANCE =  6.16 ANGSTROMS
REMARK 525  0 HOH  1217        DISTANCE =  5.62 ANGSTROMS
REMARK 525  0 HOH  1240        DISTANCE =  8.25 ANGSTROMS
REMARK 525  0 HOH  1251        DISTANCE =  5.69 ANGSTROMS
REMARK 525  0 HOH  1263        DISTANCE =  5.25 ANGSTROMS
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 1CV2   RELATED DB: PDB
REMARK 999
REMARK 999  SEQUENCE REFERENCE :
REMARK 999   NAGATA, Y. ET AL., (1994) J. BACTERIOL.  176, PP 3117-3125
REMARK 999
DBREF  1D07 A    1   296  GB     4521186  BAA03443         1    296
SEQRES   1 A  296  MET SER LEU GLY ALA LYS PRO PHE GLY GLU LYS LYS PHE
SEQRES   2 A  296  ILE GLU ILE LYS GLY ARG ARG MET ALA TYR ILE ASP GLU
SEQRES   3 A  296  GLY THR GLY ASP PRO ILE LEU PHE GLN HIS GLY ASN PRO
SEQRES   4 A  296  THR SER SER TYR LEU TRP ARG ASN ILE MET PRO HIS CYS
SEQRES   5 A  296  ALA GLY LEU GLY ARG LEU ILE ALA CYS ASP LEU ILE GLY
SEQRES   6 A  296  MET GLY ASP SER ASP LYS LEU ASP PRO SER GLY PRO GLU
SEQRES   7 A  296  ARG TYR ALA TYR ALA GLU HIS ARG ASP TYR LEU ASP ALA
SEQRES   8 A  296  LEU TRP GLU ALA LEU ASP LEU GLY ASP ARG VAL VAL LEU
SEQRES   9 A  296  VAL VAL HIS ASP TRP GLY SER ALA LEU GLY PHE ASP TRP
SEQRES  10 A  296  ALA ARG ARG HIS ARG GLU ARG VAL GLN GLY ILE ALA TYR
SEQRES  11 A  296  MET GLU ALA ILE ALA MET PRO ILE GLU TRP ALA ASP PHE
SEQRES  12 A  296  PRO GLU GLN ASP ARG ASP LEU PHE GLN ALA PHE ARG SER
SEQRES  13 A  296  GLN ALA GLY GLU GLU LEU VAL LEU GLN ASP ASN VAL PHE
SEQRES  14 A  296  VAL GLU GLN VAL LEU PRO GLY LEU ILE LEU ARG PRO LEU
SEQRES  15 A  296  SER GLU ALA GLU MET ALA ALA TYR ARG GLU PRO PHE LEU
SEQRES  16 A  296  ALA ALA GLY GLU ALA ARG ARG PRO THR LEU SER TRP PRO
SEQRES  17 A  296  ARG GLN ILE PRO ILE ALA GLY THR PRO ALA ASP VAL VAL
SEQRES  18 A  296  ALA ILE ALA ARG ASP TYR ALA GLY TRP LEU SER GLU SER
SEQRES  19 A  296  PRO ILE PRO LYS LEU PHE ILE ASN ALA GLU PRO GLY ALA
SEQRES  20 A  296  LEU THR THR GLY ARG MET ARG ASP PHE CYS ARG THR TRP
SEQRES  21 A  296  PRO ASN GLN THR GLU ILE THR VAL ALA GLY ALA HIS PHE
SEQRES  22 A  296  ILE GLN GLU ASP SER PRO ASP GLU ILE GLY ALA ALA ILE
SEQRES  23 A  296  ALA ALA PHE VAL ARG ARG LEU ARG PRO ALA
HET     BR    901       1
HET     BR    902       1
HET    GOL    903       5
HETNAM      BR BROMIDE ION
HETNAM     GOL GLYCEROL
FORMUL   2   BR    2(BR1 1-)
FORMUL   4  GOL    C3 H8 O3
FORMUL   5  HOH   *269(H2 O1)
HELIX    1   1 SER A   42  ALA A   53  5                                  12
HELIX    2   2 TYR A   82  LEU A   96  1                                  15
HELIX    3   3 TRP A  109  ARG A  120  1                                  12
HELIX    4   4 GLU A  145  ARG A  155  1                                  11
HELIX    5   5 GLY A  159  LEU A  164  1                                   6
HELIX    6   6 VAL A  168  LEU A  177  1                                  10
HELIX    7   7 GLU A  184  GLU A  192  1                                   9
HELIX    8   8 ARG A  202  ILE A  211  1                                  10
HELIX    9   9 ALA A  218  SER A  234  1                                  17
HELIX   10  10 THR A  250  ARG A  258  1                                   9
HELIX   11  11 ILE A  274  ASP A  277  5                                   4
HELIX   12  12 SER A  278  LEU A  293  1                                  16
SHEET    1  S1 8 LYS A  12  ILE A  14  0
SHEET    2  S1 8 MET A  21  GLU A  26 -1  N  MET A  21   O  ILE A  14
SHEET    3  S1 8 ARG A  57  ASP A  62 -1  N  ALA A  60   O  ILE A  24
SHEET    4  S1 8 ASP A  30  HIS A  36  1  N  ILE A  32   O  ARG A  57
SHEET    5  S1 8 VAL A 102  HIS A 107  1  N  VAL A 103   O  PRO A  31
SHEET    6  S1 8 VAL A 125  MET A 131  1  N  ALA A 129   O  LEU A 104
SHEET    7  S1 8 LYS A 238  PRO A 245  1  N  ILE A 241   O  TYR A 130
SHEET    8  S1 8 GLN A 263  GLY A 270  1  N  THR A 264   O  LYS A 238
CISPEP   1 ASN A   38    PRO A   39          0        -3.10
CISPEP   2 ASP A   73    PRO A   74          0         0.90
CISPEP   3 THR A  216    PRO A  217          0         1.46
CISPEP   4 GLU A  244    PRO A  245          0         9.53
CISPEP   5 PRO A  295    ALA A  296          0        -1.64
CRYST1   50.227   71.721   73.319  90.00  90.00  90.00 P 21 21 2     4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.019910  0.000000  0.000000        0.00000
SCALE2      0.000000  0.013943  0.000000        0.00000
SCALE3      0.000000  0.000000  0.013639        0.00000
END