longtext: 1PJA-pdb

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HEADER    HYDROLASE                               02-JUN-03   1PJA
TITLE     THE CRYSTAL STRUCTURE OF PALMITOYL PROTEIN THIOESTERASE-2
TITLE    2 REVEALS THE BASIS FOR DIVERGENT SUBSTRATE SPECIFICITIES OF
TITLE    3 THE TWO LYSOSOMAL THIOESTERASES (PPT1 AND PPT2)
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: PALMITOYL-PROTEIN THIOESTERASE 2 PRECURSOR;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: PALMITOYL- PROTEIN HYDROLASE 2, PPT-2, G14;
COMPND   5 EC: 3.1.2.22;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE   3 ORGANISM_COMMON: HUMAN;
SOURCE   4 GENE: PPT2;
SOURCE   5 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;
SOURCE   6 EXPRESSION_SYSTEM_COMMON: FALL ARMYWORM;
SOURCE   7 EXPRESSION_SYSTEM_VECTOR_TYPE: BACULOVIRUS
KEYWDS    HYDROLASE, GLYCOPROTEIN, LYSOSOME
EXPDTA    X-RAY DIFFRACTION
AUTHOR    G.CALERO,P.GUPTA,M.C.NONATO,S.TANDEL,E.R.BIEHL,S.L.HOFMANN,
AUTHOR   2 J.CLARDY
REVDAT   1   02-SEP-03 1PJA    0
JRNL        AUTH   G.CALERO,P.GUPTA,M.C.NONATO,S.TANDEL,E.R.BIEHL,
JRNL        AUTH 2 S.L.HOFMANN,J.CLARDY
JRNL        TITL   THE CRYSTAL STRUCTURE OF PALMITOYL PROTEIN
JRNL        TITL 2 THIOESTERASE-2 REVEALS THE BASIS FOR DIVERGENT
JRNL        TITL 3 SUBSTRATE SPECIFICITIES OF THE TWO LYSOSOMAL
JRNL        TITL 4 THIOESTERASES (PPT1 AND PPT2)
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   1
REMARK   2
REMARK   2 RESOLUTION. 2.70 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : CNS
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,
REMARK   3               : READ,RICE,SIMONSON,WARREN
REMARK   3
REMARK   3  REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.70
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 40.00
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : NULL
REMARK   3   NUMBER OF REFLECTIONS             : 27511
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : NULL
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL
REMARK   3   R VALUE            (WORKING SET) : 0.221
REMARK   3   FREE R VALUE                     : 0.242
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : NULL
REMARK   3   FREE R VALUE TEST SET COUNT      : NULL
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL
REMARK   3   BIN R VALUE           (WORKING SET) : NULL
REMARK   3   BIN FREE R VALUE                    : NULL
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 2161
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 14
REMARK   3   SOLVENT ATOMS            : 68
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL
REMARK   3   ESD FROM SIGMAA              (A) : NULL
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL
REMARK   3
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.
REMARK   3   BOND LENGTHS                 (A) : NULL
REMARK   3   BOND ANGLES            (DEGREES) : NULL
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : NULL
REMARK   3   IMPROPER ANGLES        (DEGREES) : NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL MODEL : NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELING.
REMARK   3   METHOD USED : NULL
REMARK   3   KSOL        : NULL
REMARK   3   BSOL        : NULL
REMARK   3
REMARK   3  NCS MODEL : NULL
REMARK   3
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  PARAMETER FILE  1  : NULL
REMARK   3  TOPOLOGY FILE  1   : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 1PJA COMPLIES WITH FORMAT V. 2.3, 09-JULY-1998
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 11-AUG-2003.
REMARK 100 THE RCSB ID CODE IS RCSB019357.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : NULL
REMARK 200  TEMPERATURE           (KELVIN) : NULL
REMARK 200  PH                             : 6.00
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : CHESS
REMARK 200  BEAMLINE                       : A1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.943
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : MIRRORS
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 4
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 34721
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.700
REMARK 200  RESOLUTION RANGE LOW       (A) : 40.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8
REMARK 200  DATA REDUNDANCY                : 6.500
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : 0.04600
REMARK 200   FOR THE DATA SET  : 15.7000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.70
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 2.500
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): NULL
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): NULL
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 1.8-2.2M AMMONIUM SULFATE, 8%
REMARK 280  METHYL-PENTANE-DIOL, 100 MM MES , PH 6.0, VAPOR DIFFUSION,
REMARK 280  HANGING DROP, TEMPERATURE 277K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 62 2 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -Y,X-Y,2/3+Z
REMARK 290       3555   -X+Y,-X,1/3+Z
REMARK 290       4555   -X,-Y,Z
REMARK 290       5555   Y,-X+Y,2/3+Z
REMARK 290       6555   X-Y,X,1/3+Z
REMARK 290       7555   Y,X,2/3-Z
REMARK 290       8555   X-Y,-Y,-Z
REMARK 290       9555   -X,-X+Y,1/3-Z
REMARK 290      10555   -Y,-X,2/3-Z
REMARK 290      11555   -X+Y,Y,-Z
REMARK 290      12555   X,X-Y,1/3-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      101.67333
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       50.83667
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000      101.67333
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       50.83667
REMARK 290   SMTRY1   7 -0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   7  0.866025  0.500000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000      101.67333
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   9 -0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   9 -0.866025  0.500000  0.000000        0.00000
REMARK 290   SMTRY3   9  0.000000  0.000000 -1.000000       50.83667
REMARK 290   SMTRY1  10  0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2  10 -0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3  10  0.000000  0.000000 -1.000000      101.67333
REMARK 290   SMTRY1  11 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2  11  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3  11  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1  12  0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2  12  0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3  12  0.000000  0.000000 -1.000000       50.83667
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT
REMARK 300 WHICH CONSISTS OF 1 CHAIN(S). SEE REMARK 350 FOR
REMARK 300 INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S).
REMARK 350
REMARK 350 GENERATING THE BIOMOLECULE
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     LEU A     2
REMARK 465     GLY A     3
REMARK 465     LEU A     4
REMARK 465     TRP A     5
REMARK 465     GLY A     6
REMARK 465     GLN A     7
REMARK 465     ARG A     8
REMARK 465     LEU A     9
REMARK 465     PRO A    10
REMARK 465     ALA A    11
REMARK 465     ALA A    12
REMARK 465     TRP A    13
REMARK 465     VAL A    14
REMARK 465     LEU A    15
REMARK 465     LEU A    16
REMARK 465     LEU A    17
REMARK 465     LEU A    18
REMARK 465     PRO A    19
REMARK 465     PHE A    20
REMARK 465     LEU A    21
REMARK 465     PRO A    22
REMARK 465     LEU A    23
REMARK 465     LEU A    24
REMARK 465     LEU A    25
REMARK 465     LEU A    26
REMARK 465     ALA A    27
REMARK 465     ALA A    28
REMARK 465     PRO A    29
REMARK 465     ALA A    30
REMARK 465     PRO A    31
REMARK 465     HIS A    32
REMARK 465     ARG A    33
REMARK 465     ALA A    34
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI
REMARK 500   N    GLY A    76     O    HOH      11              2.06
REMARK 500   NE2  HIS A   287     O    HOH      47              2.15
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   OE2  GLU A   172     OE2  GLU A   172    10665     2.12
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    ARG A 163   CB    ARG A 163   CG     0.045
REMARK 500    ARG A 163   CG    ARG A 163   CD     0.047
REMARK 500    ASN A 190   CG    ASN A 190   ND2   -0.047
REMARK 500    HIS A 219   CB    HIS A 219   CG     0.047
REMARK 500    PRO A 233   C     TRP A 234   N      0.094
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ASP A  75   N   -  CA  -  C   ANGL. DEV. = 12.4 DEGREES
REMARK 500    ILE A 108   N   -  CA  -  C   ANGL. DEV. = -9.4 DEGREES
REMARK 500    ASP A 228   N   -  CA  -  C   ANGL. DEV. =-14.8 DEGREES
REMARK 500    GLN A 235   OE1 -  CD  -  NE2 ANGL. DEV. = -9.7 DEGREES
REMARK 500    GLN A 235   CG  -  CD  -  NE2 ANGL. DEV. =  9.4 DEGREES
REMARK 500    ASP A 243   N   -  CA  -  C   ANGL. DEV. =-10.5 DEGREES
REMARK 500    THR A 247   N   -  CA  -  C   ANGL. DEV. = -9.3 DEGREES
REMARK 500    CYS A 296   N   -  CA  -  C   ANGL. DEV. =  8.8 DEGREES
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 1EI9   RELATED DB: PDB
REMARK 900 PALMITOYL PROTEIN THIOESTERASE-1 (PPT1) LYSOSOMAL
REMARK 900 THIOESTERASES
DBREF  1PJA A    1   302  SWS    Q9UMR5   PPT2_HUMAN       1    302
SEQRES   1 A  302  MET LEU GLY LEU TRP GLY GLN ARG LEU PRO ALA ALA TRP
SEQRES   2 A  302  VAL LEU LEU LEU LEU PRO PHE LEU PRO LEU LEU LEU LEU
SEQRES   3 A  302  ALA ALA PRO ALA PRO HIS ARG ALA SER TYR LYS PRO VAL
SEQRES   4 A  302  ILE VAL VAL HIS GLY LEU PHE ASP SER SER TYR SER PHE
SEQRES   5 A  302  ARG HIS LEU LEU GLU TYR ILE ASN GLU THR HIS PRO GLY
SEQRES   6 A  302  THR VAL VAL THR VAL LEU ASP LEU PHE ASP GLY ARG GLU
SEQRES   7 A  302  SER LEU ARG PRO LEU TRP GLU GLN VAL GLN GLY PHE ARG
SEQRES   8 A  302  GLU ALA VAL VAL PRO ILE MET ALA LYS ALA PRO GLN GLY
SEQRES   9 A  302  VAL HIS LEU ILE CYS TYR SER GLN GLY GLY LEU VAL CYS
SEQRES  10 A  302  ARG ALA LEU LEU SER VAL MET ASP ASP HIS ASN VAL ASP
SEQRES  11 A  302  SER PHE ILE SER LEU SER SER PRO GLN MET GLY GLN TYR
SEQRES  12 A  302  GLY ASP THR ASP TYR LEU LYS TRP LEU PHE PRO THR SER
SEQRES  13 A  302  MET ARG SER ASN LEU TYR ARG ILE CYS TYR SER PRO TRP
SEQRES  14 A  302  GLY GLN GLU PHE SER ILE CYS ASN TYR TRP HIS ASP PRO
SEQRES  15 A  302  HIS HIS ASP ASP LEU TYR LEU ASN ALA SER SER PHE LEU
SEQRES  16 A  302  ALA LEU ILE ASN GLY GLU ARG ASP HIS PRO ASN ALA THR
SEQRES  17 A  302  VAL TRP ARG LYS ASN PHE LEU ARG VAL GLY HIS LEU VAL
SEQRES  18 A  302  LEU ILE GLY GLY PRO ASP ASP GLY VAL ILE THR PRO TRP
SEQRES  19 A  302  GLN SER SER PHE PHE GLY PHE TYR ASP ALA ASN GLU THR
SEQRES  20 A  302  VAL LEU GLU MET GLU GLU GLN LEU VAL TYR LEU ARG ASP
SEQRES  21 A  302  SER PHE GLY LEU LYS THR LEU LEU ALA ARG GLY ALA ILE
SEQRES  22 A  302  VAL ARG CYS PRO MET ALA GLY ILE SER HIS THR ALA TRP
SEQRES  23 A  302  HIS SER ASN ARG THR LEU TYR GLU THR CYS ILE GLU PRO
SEQRES  24 A  302  TRP LEU SER
HET    NAG   2285      14
HET    GOL   1614       6
HET    GOL   1615       6
HET    GOL   1616       6
HETNAM     NAG N-ACETYL-D-GLUCOSAMINE
HETNAM     GOL GLYCEROL
HETSYN     NAG NAG
FORMUL   2  NAG    C8 H15 N1 O6
FORMUL   3  GOL    3(C3 H8 O3)
FORMUL   6  HOH   *50(H2 O1)
HELIX    1   1 SER A   48  SER A   51  5                                   4
HELIX    2   2 PHE A   52  HIS A   63  1                                  12
HELIX    3   3 GLY A   76  ARG A   81  5                                   6
HELIX    4   4 PRO A   82  ALA A  101  1                                  20
HELIX    5   5 SER A  111  MET A  124  1                                  14
HELIX    6   6 THR A  146  PHE A  153  1                                   8
HELIX    7   7 MET A  157  TYR A  166  1                                  10
HELIX    8   8 TRP A  169  PHE A  173  5                                   5
HELIX    9   9 SER A  174  TRP A  179  5                                   6
HELIX   10  10 HIS A  184  SER A  192  1                                   9
HELIX   11  11 PHE A  194  ASN A  199  1                                   6
HELIX   12  12 ASN A  206  LEU A  215  1                                  10
HELIX   13  13 PRO A  233  PHE A  239  5                                   7
HELIX   14  14 GLU A  250  GLU A  253  5                                   4
HELIX   15  15 GLN A  254  ARG A  259  1                                   6
HELIX   16  16 GLY A  263  ARG A  270  1                                   8
HELIX   17  17 ASN A  289  ILE A  297  1                                   9
HELIX   18  18 GLU A  298  LEU A  301  5                                   4
SHEET    1   A 6 VAL A  68  VAL A  70  0
SHEET    2   A 6 VAL A  39  VAL A  42  1  N  VAL A  39   O  THR A  69
SHEET    3   A 6 VAL A 105  TYR A 110  1  O  TYR A 110   N  VAL A  42
SHEET    4   A 6 VAL A 129  LEU A 135  1  O  ILE A 133   N  LEU A 107
SHEET    5   A 6 HIS A 219  GLY A 224  1  O  HIS A 219   N  PHE A 132
SHEET    6   A 6 ILE A 273  PRO A 277  1  O  CYS A 276   N  LEU A 222
SHEET    1   B 2 PHE A 241  TYR A 242  0
SHEET    2   B 2 VAL A 248  LEU A 249 -1  O  LEU A 249   N  PHE A 241
SSBOND   1 CYS A  109    CYS A  117
SSBOND   2 CYS A  165    CYS A  176
SSBOND   3 CYS A  276    CYS A  296
CISPEP   1 THR A  232    PRO A  233          0        -0.37
CRYST1  148.520  148.520  152.510  90.00  90.00 120.00 P 62 2 2     12
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.006733  0.003887  0.000000        0.00000
SCALE2      0.000000  0.007775  0.000000        0.00000
SCALE3      0.000000  0.000000  0.006557        0.00000
TER    2162      SER A 302
MASTER      355    0    4   18    8    0    0    6 2243    1   38   24
END