longtext: 1QJ4-pdb

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HEADER    LYASE                                   21-JUN-99   1QJ4
TITLE     HYDROXYNITRILE-LYASE FROM HEVEA BRASILIENSIS AT ATOMIC
TITLE    2 RESOLUTION
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: HYDROXYNITRILE LYASE;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: OXYNITRILE LYASE;
COMPND   5 EC: 4.2.1.39;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: HEVEA BRASILIENSIS;
SOURCE   3 ORGANISM_COMMON: PARA RUBBER TREE;
SOURCE   4 ORGAN: LEAF;
SOURCE   5 PLASMID: BHIL-D2;
SOURCE   6 GENE: HNL;
SOURCE   7 EXPRESSION_SYSTEM: PICHIA PASTORIS;
SOURCE   8 EXPRESSION_SYSTEM_PLASMID: BHIL-D2;
SOURCE   9 EXPRESSION_SYSTEM_GENE: HNL
KEYWDS    OXYNITRILASE, CYANOGENESIS, CYANHYDRIN FORMATION, LYASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    M.GUGGANIG,K.GRUBER,C.KRATKY
REVDAT   1   10-OCT-99 1QJ4    0
JRNL        AUTH   K.GRUBER,M.GUGGANIG,U.G.WAGNER,C.KRATKY
JRNL        TITL   ATOMIC RESOLUTION CRYSTAL STRUCTURE OF
JRNL        TITL 2 HYDROXYNITRILE LYASE FROM HEVEA BRASILIENSIS
JRNL        REF    BIOL.CHEM.                    V.  380  993 1999
JRNL        REFN   ASTM BICHF3  GE ISSN 1431-6730                 2169
REMARK   1
REMARK   1 REFERENCE 1
REMARK   1  AUTH   J.ZUEGG,K.GRUBER,M.GUGGANIG,U.G.WAGNER,C.KRATKY
REMARK   1  TITL   3D STRUCTURES OF ENZYME-SUBSTRATE COMPLEXES OF THE
REMARK   1  TITL 2 HYDROXYNITRILE LYASE FROM HEVEA BRASILIENSIS
REMARK   1  REF    TO BE PUBLISHED
REMARK   1  REFN                                                  0353
REMARK   1 REFERENCE 2
REMARK   1  AUTH   U.G.WAGNER,M.HASSLACHER,H.GRIENGL,H.SCHWAB,
REMARK   1  AUTH 2 C.KRATKY
REMARK   1  TITL   MECHANISM OF CYANOGENESIS: THE CRYSTAL STRUCTURE OF
REMARK   1  TITL 2 HYDROXYNITRILE LYASE FROM HEVEA BRASILIENSIS
REMARK   1  REF    STRUCTURE (LONDON)            V.   4   811 1996
REMARK   1  REFN   ASTM STRUE6  UK ISSN 0969-2126                 2005
REMARK   1 REFERENCE 3
REMARK   1  AUTH   U.G.WAGNER,M.SCHALL,M.HAYN,M.HASSLACHER,H.SCHWAB,
REMARK   1  AUTH 2 H.S.GRIENGL,C.KRATKY
REMARK   1  TITL   CRYSTALLIZATION OF A HYDROXYNITRILE LYASE
REMARK   1  REF    ACTA CRYSTALLOGR.,SECT.D      V.  52   591 1996
REMARK   1  REFN   ASTM ABCRE6  DK ISSN 0907-4449                 0766
REMARK   1 REFERENCE 4
REMARK   1  AUTH   M.HASSLACHER,M.SCHALL,M.HAYN,H.GRIENGL,
REMARK   1  AUTH 2 S.D.KOHLWEIN,H.SCHWAB
REMARK   1  TITL   MOLECULAR CLONING OF THE FULL-LENGTH CDNA OF
REMARK   1  TITL 2 (S)-HYDROXYNITRILE LYASE FROM HEVEA BRASILIENSIS.
REMARK   1  TITL 3 FUNCTIONAL EXPRESSION IN ESCHERICHIA COLI AND
REMARK   1  TITL 4 SACCHAROMYCES CEREVISIAE AND IDENTIFICATION OF AN
REMARK   1  TITL 5 ACTIVE SITE RESIDUE
REMARK   1  REF    J.BIOL.CHEM.                  V. 271  5884 1996
REMARK   1  REFN   ASTM JBCHA3  US ISSN 0021-9258                 0071
REMARK   2
REMARK   2 RESOLUTION. 1.1  ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : SHELXL-97
REMARK   3   AUTHORS     : G.M.SHELDRICK
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.10
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 50.00
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.0
REMARK   3   COMPLETENESS FOR RANGE        (%) : 94.1
REMARK   3   CROSS-VALIDATION METHOD           : FREE R
REMARK   3   FREE R VALUE TEST SET SELECTION   : RANDOM
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (NO CUTOFF).
REMARK   3   R VALUE   (WORKING + TEST SET, NO CUTOFF) : 0.1158
REMARK   3   R VALUE          (WORKING SET, NO CUTOFF) : 0.1149
REMARK   3   FREE R VALUE                  (NO CUTOFF) : 0.1437
REMARK   3   FREE R VALUE TEST SET SIZE (%, NO CUTOFF) : 5.0
REMARK   3   FREE R VALUE TEST SET COUNT   (NO CUTOFF) : 6192
REMARK   3   TOTAL NUMBER OF REFLECTIONS   (NO CUTOFF) : 123339
REMARK   3
REMARK   3  FIT/AGREEMENT OF MODEL FOR DATA WITH F>4SIG(F).
REMARK   3   R VALUE   (WORKING + TEST SET, F>4SIG(F)) : 0.1083
REMARK   3   R VALUE          (WORKING SET, F>4SIG(F)) : 0.1075
REMARK   3   FREE R VALUE                  (F>4SIG(F)) : 0.1353
REMARK   3   FREE R VALUE TEST SET SIZE (%, F>4SIG(F)) : 5.0
REMARK   3   FREE R VALUE TEST SET COUNT   (F>4SIG(F)) : 5248
REMARK   3   TOTAL NUMBER OF REFLECTIONS   (F>4SIG(F)) : 104710
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 2155
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 32
REMARK   3   SOLVENT ATOMS            : 550
REMARK   3
REMARK   3  MODEL REFINEMENT.
REMARK   3   OCCUPANCY SUM OF NON-HYDROGEN ATOMS      : 2629.75
REMARK   3   OCCUPANCY SUM OF HYDROGEN ATOMS          : 2053.00
REMARK   3   NUMBER OF DISCRETELY DISORDERED RESIDUES : 25
REMARK   3   NUMBER OF LEAST-SQUARES PARAMETERS       : 24572
REMARK   3   NUMBER OF RESTRAINTS                     : 43482
REMARK   3
REMARK   3  RMS DEVIATIONS FROM RESTRAINT TARGET VALUES.
REMARK   3   BOND LENGTHS                         (A) : 0.014
REMARK   3   ANGLE DISTANCES                      (A) : 0.030
REMARK   3   SIMILAR DISTANCES (NO TARGET VALUES) (A) : 0.000
REMARK   3   DISTANCES FROM RESTRAINT PLANES      (A) : 0.0281
REMARK   3   ZERO CHIRAL VOLUMES               (A**3) : 0.086
REMARK   3   NON-ZERO CHIRAL VOLUMES           (A**3) : 0.095
REMARK   3   ANTI-BUMPING DISTANCE RESTRAINTS     (A) : 0.109
REMARK   3   RIGID-BOND ADP COMPONENTS         (A**2) : 0.005
REMARK   3   SIMILAR ADP COMPONENTS            (A**2) : 0.029
REMARK   3   APPROXIMATELY ISOTROPIC ADPS      (A**2) : 0.059
REMARK   3
REMARK   3  BULK SOLVENT MODELING.
REMARK   3   METHOD USED: MOEWS & KRETSINGER, J.MOL.BIOL.91(1973)201-2
REMARK   3
REMARK   3  STEREOCHEMISTRY TARGET VALUES : ENGH AND HUBER
REMARK   3   SPECIAL CASE: NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: ANISOTROPIC REFINEMENT REDUCED
REMARK   3   FREE R (NO CUTOFF) BY 0.043.
REMARK   3   RESIDUE SER-A80 IS OBSERVED IN A RAMACHANDRAN 'FORBIDDEN'
REMARK   3   REGION. THIS CONFORMATION IS FOUND IN ALL PREVIOUS
REMARK   3   HNL-STRUCTURES. STRUCTURES OF OTHER MEMBERS OF THE
REMARK   3   ALPHA/BETA HYDROLASES FAMILY ALSO CONSISTENTLY SHOW
REMARK   3   THE EQUIVALENT RESIDUE (NUCLEOPHILE) IN SUCH A
REMARK   3   CONFORMATION.
REMARK   4
REMARK   4 1QJ4 COMPLIES WITH FORMAT V. 2.3, 09-JULY-1998
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY EBI  ON 21-JUN-1999.
REMARK 100 THE EBI ID CODE IS EBI-2844.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 15-AUG-98
REMARK 200  TEMPERATURE           (KELVIN) : 120
REMARK 200  PH                             : 7.4
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : EMBL/DESY,HAMBURG
REMARK 200  BEAMLINE                       : BW7B
REMARK 200  X-RAY GENERATOR MODEL          : NONE
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.8345
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : MAR-345
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 123339
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.1
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.0
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.0
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 94.1
REMARK 200  DATA REDUNDANCY                : 4.1
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : 0.065
REMARK 200   FOR THE DATA SET  : 20.4
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.10
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.11
REMARK 200  COMPLETENESS FOR SHELL     (%) : 92.3
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.8
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : 0.364
REMARK 200   FOR SHELL         : 3.0
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: OTHER
REMARK 200 SOFTWARE USED: SHELXL-97
REMARK 200 STARTING MODEL: 7YAS
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS  (%): 56.
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.8
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M NATRIUM HEPES BUFFER PH=7.4
REMARK 280  2 % PEG 400, 2.0 M AMMONIUM SULFATE, ROOM-TEMP.
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP:  C 2 2 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,1/2+Z
REMARK 290       3555   -X,Y,1/2-Z
REMARK 290       4555   X,-Y,-Z
REMARK 290       5555   1/2+X,1/2+Y,Z
REMARK 290       6555   1/2-X,1/2-Y,1/2+Z
REMARK 290       7555   1/2-X,1/2+Y,1/2-Z
REMARK 290       8555   1/2+X,1/2-Y,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       64.08000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       64.08000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       23.64350
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       53.32750
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       23.64350
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       53.32750
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       64.08000
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       23.64350
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       53.32750
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       64.08000
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       23.64350
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       53.32750
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE
REMARK 300 A XRAY OBSERVED BIO-ASSEMBLY CAN BE BUILT
REMARK 300 BY SPACE GROUP SYMMETRY EXPANSION OF THE
REMARK 300 CONTENTS OF THE ASYMMETRIC UNIT
REMARK 300
REMARK 300 FOR THE HOMO-ASSEMBLY DESCRIBED BY REMARK 350
REMARK 300 THE DIFFERENCE IN ACCESSIBLE SURFACE AREA PER
REMARK 300 CHAIN BETWEEN THE ISOLATED CHAIN AND THAT FOR
REMARK 300 THE CHAIN IN THE COMPLEX IS 814.3 ANGSTROM**2
REMARK 350
REMARK 350 GENERATING THE BIOMOLECULE
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 AN OLIGOMER OF TYPE :DIMERIC : CAN BE ASSEMBLED BY
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1  1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2  1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3  1  0.000000  0.000000  1.000000        0.00000
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1  2  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2  2  0.000000 -1.000000  0.000000      106.65500
REMARK 350   BIOMT3  2  0.000000  0.000000 -1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH   509    LIES ON A SPECIAL POSITION.
REMARK 375      HOH   596    LIES ON A SPECIAL POSITION.
REMARK 375      HOH   700    LIES ON A SPECIAL POSITION.
REMARK 375      HOH   737    LIES ON A SPECIAL POSITION.
REMARK 375      HOH   873    LIES ON A SPECIAL POSITION.
REMARK 375      HOH   990    LIES ON A SPECIAL POSITION.
REMARK 375
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500
REMARK 500  O     HOH     535    O     HOH     758               2.13
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500
REMARK 500    SER A  80       55.02   -113.35
REMARK 650
REMARK 650 HELIX
REMARK 650 DETERMINATION METHOD: DSSP
REMARK 700
REMARK 700 SHEET
REMARK 700 DETERMINATION METHOD: DSSP
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: CAT
REMARK 800 SITE_DESCRIPTION: CATALYTIC TRIAD
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 1YAS   RELATED DB: PDB
REMARK 900
DBREF  1QJ4 A    1   257  SWS    P52704   HNL_HEVBR        1    257
SEQRES   1 A  257  MET ALA PHE ALA HIS PHE VAL LEU ILE HIS THR ILE CYS
SEQRES   2 A  257  HIS GLY ALA TRP ILE TRP HIS LYS LEU LYS PRO LEU LEU
SEQRES   3 A  257  GLU ALA LEU GLY HIS LYS VAL THR ALA LEU ASP LEU ALA
SEQRES   4 A  257  ALA SER GLY VAL ASP PRO ARG GLN ILE GLU GLU ILE GLY
SEQRES   5 A  257  SER PHE ASP GLU TYR SER GLU PRO LEU LEU THR PHE LEU
SEQRES   6 A  257  GLU ALA LEU PRO PRO GLY GLU LYS VAL ILE LEU VAL GLY
SEQRES   7 A  257  GLU SER CYS GLY GLY LEU ASN ILE ALA ILE ALA ALA ASP
SEQRES   8 A  257  LYS TYR CYS GLU LYS ILE ALA ALA ALA VAL PHE HIS ASN
SEQRES   9 A  257  SER VAL LEU PRO ASP THR GLU HIS CYS PRO SER TYR VAL
SEQRES  10 A  257  VAL ASP LYS LEU MET GLU VAL PHE PRO ASP TRP LYS ASP
SEQRES  11 A  257  THR THR TYR PHE THR TYR THR LYS ASP GLY LYS GLU ILE
SEQRES  12 A  257  THR GLY LEU LYS LEU GLY PHE THR LEU LEU ARG GLU ASN
SEQRES  13 A  257  LEU TYR THR LEU CYS GLY PRO GLU GLU TYR GLU LEU ALA
SEQRES  14 A  257  LYS MET LEU THR ARG LYS GLY SER LEU PHE GLN ASN ILE
SEQRES  15 A  257  LEU ALA LYS ARG PRO PHE PHE THR LYS GLU GLY TYR GLY
SEQRES  16 A  257  SER ILE LYS LYS ILE TYR VAL TRP THR ASP GLN ASP GLU
SEQRES  17 A  257  ILE PHE LEU PRO GLU PHE GLN LEU TRP GLN ILE GLU ASN
SEQRES  18 A  257  TYR LYS PRO ASP LYS VAL TYR LYS VAL GLU GLY GLY ASP
SEQRES  19 A  257  HIS LYS LEU GLN LEU THR LYS THR LYS GLU ILE ALA GLU
SEQRES  20 A  257  ILE LEU GLN GLU VAL ALA ASP THR TYR ASN
HET    GOL    300       6
HET    SO4    400       5
HET    SO4    401       5
HET    SO4    402       5
HET    SO4    403       5
HETNAM     GOL GLYCEROL
HETNAM     SO4 SULFATE ION
HETSYN     GOL 1,2,3-PROPANETRIOL
FORMUL   2  GOL    C3 H8 O3
FORMUL   3  SO4    4(O4 S1 2-)
FORMUL   4  HOH   *550(H2 O1)
HELIX    1   1 GLY A   15  HIS A   20  5                                   6
HELIX    2   2 LYS A   21  LEU A   29  1                                   9
HELIX    3   3 GLN A   47  ILE A   51  5                                   5
HELIX    4   4 SER A   53  SER A   58  1                                   6
HELIX    5   5 SER A   58  ALA A   67  1                                  10
HELIX    6   6 CYS A   81  CYS A   94  1                                  14
HELIX    7   7 SER A  115  PHE A  125  1                                  11
HELIX    8   8 GLY A  149  LEU A  157  1                                   9
HELIX    9   9 GLY A  162  THR A  173  1                                  12
HELIX   10  10 PHE A  179  ARG A  186  1                                   8
HELIX   11  11 GLY A  193  ILE A  197  5                                   5
HELIX   12  12 LEU A  211  TYR A  222  1                                  12
HELIX   13  13 LYS A  236  LYS A  241  1                                   6
HELIX   14  14 LYS A  241  TYR A  256  1                                  16
SHEET    1  S1 6 ALA A   4  ILE A   9  0
SHEET    2  S1 6 LYS A  32  LEU A  36  1
SHEET    3  S1 6 SER A  53  LEU A  68 -1
SHEET    4  S1 6 LYS A  96  ASN A 104 -1
SHEET    5  S1 6 LYS A 198  TRP A 203  1
SHEET    6  S1 6 LYS A 226  VAL A 230  1
SHEET    1  S2 3 THR A 132  LYS A 138  0
SHEET    2  S2 3 LYS A 141  LYS A 147 -1
SHEET    3  S2 3 GLY A 176  LEU A 178 -1
SITE     1 CAT  3 SER A  80  HIS A 235  ASP A 207
CRYST1   47.287  106.655  128.160  90.00  90.00  90.00 C 2 2 21      8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.021147  0.000000  0.000000        0.00000
SCALE2      0.000000  0.009376  0.000000        0.00000
SCALE3      0.000000  0.000000  0.007803        0.00000
END