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HEADER HYDROLASE(CARBOXYLIC ESTERASE) 05-FEB-90 1TGL 1TGL 2
COMPND TRIACYLGLYCEROL ACYLHYDROLASE (E.C.3.1.1.3) 1TGL 3
SOURCE (RHIZOMUCOR $MIEHEI) 1TGLA 1
AUTHOR L.BRADY,A.M.BRZOZOWSKI,Z.S.DEREWENDA,E.J.DODSON,G.G.DODSON, 1TGL 5
AUTHOR 2 S.P.TOLLEY,J.P.TURKENBURG,L.CHRISTIANSEN,B.HUGE-*JENSEN, 1TGL 6
AUTHOR 3 L.NORSKOV,L.THIM 1TGL 7
REVDAT 2 15-JAN-91 1TGLA 1 SOURCE 1TGLA 2
REVDAT 1 15-OCT-90 1TGL 0 1TGL 8
REMARK 1 1TGL 9
REMARK 1 REFERENCE 1 1TGL 10
REMARK 1 AUTH L.BRADY,A.M.BRZOZOWSKI,Z.S.DEREWENDA,E.J.DODSON, 1TGL 11
REMARK 1 AUTH 2 G.DODSON,S.TOLLEY,J.P.TURKENBURG,L.CHRISTIANSEN, 1TGL 12
REMARK 1 AUTH 3 B.HUGE-*JENSEN,L.NORSKOV,L.THIM,U.MENGE 1TGL 13
REMARK 1 TITL A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE 1TGL 14
REMARK 1 TITL 2 OF A TRIACYLGLYCEROL LIPASE 1TGL 15
REMARK 1 REF NATURE V. 343 767 1990 1TGL 16
REMARK 1 REFN ASTM NATUAS UK ISSN 0028-0836 006 1TGL 17
REMARK 1 REFERENCE 2 1TGL 18
REMARK 1 AUTH E.BOEL,B.HUGE-*JENSEN,M.CHRISTENSEN,L.THIM,N.P.FIIL 1TGL 19
REMARK 1 TITL RHIZOMUCOR $MIEHEI TRIGLYCERIDE LIPASE IS 1TGL 20
REMARK 1 TITL 2 SYNTHESIZED AS A PRECURSOR 1TGL 21
REMARK 1 REF LIPIDS V. 23 701 1988 1TGL 22
REMARK 1 REFN ASTM LPDSAP US ISSN 0024-4201 820 1TGL 23
REMARK 2 1TGL 24
REMARK 2 RESOLUTION. 1.9 ANGSTROMS. 1TGL 25
REMARK 3 1TGL 26
REMARK 3 REFINEMENT. BY THE RESTRAINED LEAST-SQUARES PRCEDURE OF J. 1TGL 27
REMARK 3 KONNERT AND W. HENDRICKSON (PROGRAM *PROLSQ*). THE R 1TGL 28
REMARK 3 VALUE IS 0.138 FOR ALL DATA IN THE RESOLUTION RANGE 7.5 1TGL 29
REMARK 3 TO 1.9 ANGSTROMS. 1TGL 30
REMARK 4 1TGL 31
REMARK 4 CA COORDINATES ONLY ARE PRSENTED IN THIS ENTRY. A FULL 1TGL 32
REMARK 4 SET OF COORDINATES WILL BE SUBMITTED AT A LATER STAGE. 1TGL 33
REMARK 4 THE FIRST FOUR RESIDUES ARE NOT VISIBLE IN THE MAPS AND, 1TGL 34
REMARK 4 THEREFORE, NO CARBON ALPHA COORDINATES FOR THEM ARE 1TGL 35
REMARK 4 PRESENT IN THIS ENTRY. THERE ARE 269 RESIDUES,IN TOTAL, 1TGL 36
REMARK 4 IN A SINGLE CHAIN. 1TGL 37
REMARK 5 1TGL 38
REMARK 5 RESIDUE 156 IN THIS ENTRY IS ASP AS IDENTIFIED BY ELECTRON 1TGL 39
REMARK 5 DENSITY. IN THE PAPER CITED AS REFERENCE 2 ABOVE IT WAS 1TGL 40
REMARK 5 INCORRECTLY ASSIGNED AS GLY. 1TGL 41
REMARK 6 1TGL 42
REMARK 6 SITE *CAT* IS THE CATALYTIC SITE. 1TGL 43
REMARK 7 1TGLA 3
REMARK 7 CORRECTION. REVISE SPECIES NAME ON SOURCE RECORD ACCORDING 1TGLA 4
REMARK 7 TO INSTRUCTIONS FROM DEPOSITOR. 15-JAN-91. 1TGLA 5
SEQRES 1 269 SER ILE ASN GLY GLY ILE ARG ALA ALA THR SER GLN GLU 1TGL 44
SEQRES 2 269 ILE ASN GLU LEU THR TYR TYR THR THR LEU SER ALA ASN 1TGL 45
SEQRES 3 269 SER TYR CYS ARG THR VAL ILE PRO GLY ALA THR TRP ASP 1TGL 46
SEQRES 4 269 CYS ILE HIS CYS ASP ALA THR GLU ASP LEU LYS ILE ILE 1TGL 47
SEQRES 5 269 LYS THR TRP SER THR LEU ILE TYR ASP THR ASN ALA MET 1TGL 48
SEQRES 6 269 VAL ALA ARG GLY ASP SER GLU LYS THR ILE TYR ILE VAL 1TGL 49
SEQRES 7 269 PHE ARG GLY SER SER SER ILE ARG ASN TRP ILE ALA ASP 1TGL 50
SEQRES 8 269 LEU THR PHE VAL PRO VAL SER TYR PRO PRO VAL SER GLY 1TGL 51
SEQRES 9 269 THR LYS VAL HIS LYS GLY PHE LEU ASP SER TYR GLY GLU 1TGL 52
SEQRES 10 269 VAL GLN ASN GLU LEU VAL ALA THR VAL LEU ASP GLN PHE 1TGL 53
SEQRES 11 269 LYS GLN TYR PRO SER TYR LYS VAL ALA VAL THR GLY HIS 1TGL 54
SEQRES 12 269 SER LEU GLY GLY ALA THR ALA LEU LEU CYS ALA LEU ASP 1TGL 55
SEQRES 13 269 LEU TYR GLN ARG GLU GLU GLY LEU SER SER SER ASN LEU 1TGL 56
SEQRES 14 269 PHE LEU TYR THR GLN GLY GLN PRO ARG VAL GLY ASN PRO 1TGL 57
SEQRES 15 269 ALA PHE ALA ASN TYR VAL VAL SER THR GLY ILE PRO TYR 1TGL 58
SEQRES 16 269 ARG ARG THR VAL ASN GLU ARG ASP ILE VAL PRO HIS LEU 1TGL 59
SEQRES 17 269 PRO PRO ALA ALA PHE GLY PHE LEU HIS ALA GLY SER GLU 1TGL 60
SEQRES 18 269 TYR TRP ILE THR ASP ASN SER PRO GLU THR VAL GLN VAL 1TGL 61
SEQRES 19 269 CYS THR SER ASP LEU GLU THR SER ASP CYS SER ASN SER 1TGL 62
SEQRES 20 269 ILE VAL PRO PHE THR SER VAL LEU ASP HIS LEU SER TYR 1TGL 63
SEQRES 21 269 PHE GLY ILE ASN THR GLY LEU CYS SER 1TGL 64
FTNOTE 1 1TGL 65
FTNOTE 1 RESIDUES PRO 34, PRO 209, PRO 229, AND PRO 250 ARE CIS 1TGL 66
FTNOTE 1 PROLINES. 1TGL 67
SSBOND 1 CYS 29 CYS 268 1TGL 68
SSBOND 2 CYS 40 CYS 43 1TGL 69
SSBOND 3 CYS 235 CYS 244 1TGL 70
SITE 1 CAT 3 SER 144 ASP 203 HIS 257 1TGL 71
CRYST1 71.600 75.000 55.000 90.00 90.00 90.00 P 21 21 21 1 1TGL 72
ORIGX1 1.000000 0.000000 0.000000 0.00000 1TGL 73
ORIGX2 0.000000 1.000000 0.000000 0.00000 1TGL 74
ORIGX3 0.000000 0.000000 1.000000 0.00000 1TGL 75
SCALE1 0.013970 0.000000 0.000000 0.00000 1TGL 76
SCALE2 0.000000 0.013330 0.000000 0.00000 1TGL 77
SCALE3 0.000000 0.000000 0.018180 0.00000 1TGL 78 |