longtext: 2HU8-pdb

content
HEADER    HYDROLASE                               26-JUL-06   2HU8
TITLE     BINDING OF INHIBITORS BY ACYLAMINOACYL PEPTIDASE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ACYLAMINO-ACID-RELEASING ENZYME;
COMPND   3 CHAIN: A, B;
COMPND   4 SYNONYM: AARE, ACYL-PEPTIDE HYDROLASE, APH, ACYLAMINOACYL-
COMPND   5 PEPTIDASE;
COMPND   6 EC: 3.4.19.1;
COMPND   7 ENGINEERED: YES;
COMPND   8 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: AEROPYRUM PERNIX;
SOURCE   3 ORGANISM_COMMON: ARCHAEA;
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   5 EXPRESSION_SYSTEM_COMMON: BACTERIA;
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: PET22B
KEYWDS    ALPHA/BETA HYDROLASE, BETA-PROPELLER, ENZYME-INHIBITOR
KEYWDS   2 COMPLEX
EXPDTA    X-RAY DIFFRACTION
AUTHOR    A.L.KISS,B.HORNUNG,K.RADI,Z.GENGELICZKI,B.SZTARAY,V.HARMAT,
AUTHOR   2 L.POLGAR
REVDAT   1   15-MAY-07 2HU8    0
JRNL        AUTH   A.L.KISS,B.HORNUNG,K.RADI,Z.GENGELICZKI,B.SZTARAY,
JRNL        AUTH 2 T.JUHASZ,Z.SZELTNER,V.HARMAT,L.POLGAR
JRNL        TITL   THE ACYLAMINOACYL PEPTIDASE FROM AEROPYRUM PERNIX
JRNL        TITL 2 K1 THOUGHT TO BE AN EXOPEPTIDASE DISPLAYS
JRNL        TITL 3 ENDOPEPTIDASE ACTIVITY
JRNL        REF    J.MOL.BIOL.                   V. 368   509 2007
JRNL        REFN   ASTM JMOBAK  UK ISSN 0022-2836
REMARK   1
REMARK   1 REFERENCE 1
REMARK   1  AUTH   M.BARTLAM,G.WANG,H.YANG,R.GAO,X.ZHAO,G.XIE,S.CAO,
REMARK   1  AUTH 2 Y.FENG,Z.RAO
REMARK   1  TITL   CRYSTAL STRUCTURE OF AN ACYLPEPTIDE
REMARK   1  TITL 2 HYDROLASE/ESTERASE FROM AEROPYRUM PERNIX K1
REMARK   1  REF    STRUCTURE                     V.  12  1481 2004
REMARK   1  REFN   ASTM STRUE6  UK ISSN 0969-2126
REMARK   2
REMARK   2 RESOLUTION. 2.40 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.1.24
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.40
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.96
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.6
REMARK   3   NUMBER OF REFLECTIONS             : 42954
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM (AS FOR RELATED PDB
REMARK   3                                      ENTRY 2HU5)
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.218
REMARK   3   R VALUE            (WORKING SET) : 0.215
REMARK   3   FREE R VALUE                     : 0.261
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000
REMARK   3   FREE R VALUE TEST SET COUNT      : 2264
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH           : 2.40
REMARK   3   BIN RESOLUTION RANGE LOW            : 2.46
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 3075
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2820
REMARK   3   BIN FREE R VALUE SET COUNT          : 153
REMARK   3   BIN FREE R VALUE                    : 0.3470
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   ALL ATOMS                : 8890
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 5.95
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : -0.25000
REMARK   3    B22 (A**2) : -0.17000
REMARK   3    B33 (A**2) : 0.42000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.578
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.291
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.260
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 11.651
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.912
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.867
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  8861 ; 0.005 ; 0.021
REMARK   3   BOND LENGTHS OTHERS               (A):  8217 ; 0.001 ; 0.020
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 12043 ; 0.878 ; 1.970
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 18951 ; 0.887 ; 3.000
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  1162 ; 5.791 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1349 ; 0.055 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A): 10081 ; 0.002 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):  1865 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  1532 ; 0.185 ; 0.300
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  9111 ; 0.242 ; 0.300
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION OTHERS         (A):  5238 ; 0.084 ; 0.500
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   419 ; 0.195 ; 0.500
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):    14 ; 0.256 ; 0.300
REMARK   3   SYMMETRY VDW OTHERS               (A):    70 ; 0.231 ; 0.300
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):     9 ; 0.251 ; 0.500
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  5708 ; 0.061 ; 1.500
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  9120 ; 0.109 ; 2.000
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  3153 ; 0.264 ; 3.000
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  2916 ; 0.414 ; 4.500
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : 2
REMARK   3
REMARK   3  NCS GROUP NUMBER               : 1
REMARK   3     CHAIN NAMES                    : A B
REMARK   3     NUMBER OF COMPONENTS NCS GROUP : 17
REMARK   3       COMPONENT C  SSSEQI  TO  C   SSSEQI   CODE
REMARK   3           1     A     24       A      29      2
REMARK   3           1     B     24       B      29      2
REMARK   3           2     A     31       A      39      2
REMARK   3           2     B     31       B      39      2
REMARK   3           3     A     47       A      51      2
REMARK   3           3     B     47       B      51      2
REMARK   3           4     A     57       A      61      2
REMARK   3           4     B     57       B      61      2
REMARK   3           5     A     63       A      71      2
REMARK   3           5     B     63       B      71      2
REMARK   3           6     A     73       A     130      2
REMARK   3           6     B     73       B     130      2
REMARK   3           7     A    134       A     145      2
REMARK   3           7     B    134       B     145      2
REMARK   3           8     A    147       A     161      2
REMARK   3           8     B    147       B     161      2
REMARK   3           9     A    163       A     180      2
REMARK   3           9     B    163       B     180      2
REMARK   3          10     A    184       A     190      2
REMARK   3          10     B    184       B     190      2
REMARK   3          11     A    193       A     214      2
REMARK   3          11     B    193       B     214      2
REMARK   3          12     A    219       A     229      2
REMARK   3          12     B    219       B     229      2
REMARK   3          13     A    240       A     243      2
REMARK   3          13     B    240       B     243      2
REMARK   3          14     A    247       A     255      2
REMARK   3          14     B    247       B     255      2
REMARK   3          15     A    257       A     278      2
REMARK   3          15     B    257       B     278      2
REMARK   3          16     A    281       A     302      2
REMARK   3          16     B    281       B     302      2
REMARK   3          17     A    304       A     320      2
REMARK   3          17     B    304       B     320      2
REMARK   3                   GROUP CHAIN        COUNT   RMS     WEIGHT
REMARK   3   TIGHT POSITIONAL   1    A    (A):   1449 ;  0.02 ;  0.05
REMARK   3   MEDIUM POSITIONAL  1    A    (A):   2103 ;  0.28 ;  0.50
REMARK   3   TIGHT THERMAL      1    A (A**2):   1449 ;  0.02 ;  0.50
REMARK   3   MEDIUM THERMAL     1    A (A**2):   2103 ;  0.10 ;  2.00
REMARK   3
REMARK   3  NCS GROUP NUMBER               : 2
REMARK   3     CHAIN NAMES                    : A B
REMARK   3     NUMBER OF COMPONENTS NCS GROUP : 10
REMARK   3       COMPONENT C  SSSEQI  TO  C   SSSEQI   CODE
REMARK   3           1     A     10       A      13      2
REMARK   3           1     B     10       B      13      2
REMARK   3           2     A     15       A      22      2
REMARK   3           2     B     15       B      22      2
REMARK   3           3     A    323       A     344      2
REMARK   3           3     B    323       B     344      2
REMARK   3           4     A    346       A     359      2
REMARK   3           4     B    346       B     359      2
REMARK   3           5     A    362       A     380      2
REMARK   3           5     B    362       B     380      2
REMARK   3           6     A    382       A     472      2
REMARK   3           6     B    382       B     472      2
REMARK   3           7     A    474       A     496      2
REMARK   3           7     B    474       B     496      2
REMARK   3           8     A    499       A     500      2
REMARK   3           8     B    499       B     500      2
REMARK   3           9     A    502       A     505      2
REMARK   3           9     B    502       B     505      2
REMARK   3          10     A    507       A     581      2
REMARK   3          10     B    507       B     581      2
REMARK   3                   GROUP CHAIN        COUNT   RMS     WEIGHT
REMARK   3   TIGHT POSITIONAL   2    A    (A):   1523 ;  0.03 ;  0.05
REMARK   3   MEDIUM POSITIONAL  2    A    (A):   2260 ;  0.33 ;  0.50
REMARK   3   TIGHT THERMAL      2    A (A**2):   1523 ;  0.03 ;  0.50
REMARK   3   MEDIUM THERMAL     2    A (A**2):   2260 ;  0.12 ;  2.00
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 4
REMARK   3
REMARK   3   TLS GROUP : 1
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A    23        A   321
REMARK   3    ORIGIN FOR THE GROUP (A):   6.0867  40.4750  19.5147
REMARK   3    T TENSOR
REMARK   3      T11:   0.1351 T22:   0.0720
REMARK   3      T33:   0.1154 T12:  -0.0029
REMARK   3      T13:  -0.0537 T23:  -0.0028
REMARK   3    L TENSOR
REMARK   3      L11:   0.8911 L22:   1.0674
REMARK   3      L33:   1.6782 L12:   0.0780
REMARK   3      L13:   0.2444 L23:   0.0820
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0609 S12:  -0.0287 S13:   0.1812
REMARK   3      S21:  -0.0335 S22:  -0.0118 S23:  -0.0644
REMARK   3      S31:  -0.2091 S32:   0.0075 S33:   0.0727
REMARK   3
REMARK   3   TLS GROUP : 2
REMARK   3    NUMBER OF COMPONENTS GROUP : 2
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A   322        A   582
REMARK   3    RESIDUE RANGE :   A     8        A    22
REMARK   3    ORIGIN FOR THE GROUP (A):  -1.6642  13.5795  17.3314
REMARK   3    T TENSOR
REMARK   3      T11:   0.0540 T22:   0.0668
REMARK   3      T33:   0.0315 T12:   0.0002
REMARK   3      T13:  -0.0390 T23:   0.0138
REMARK   3    L TENSOR
REMARK   3      L11:   1.2305 L22:   1.7480
REMARK   3      L33:   1.3155 L12:  -0.2341
REMARK   3      L13:   0.6204 L23:  -0.0551
REMARK   3    S TENSOR
REMARK   3      S11:   0.0140 S12:  -0.0040 S13:  -0.0811
REMARK   3      S21:  -0.0204 S22:  -0.0490 S23:  -0.0905
REMARK   3      S31:   0.0982 S32:  -0.0170 S33:   0.0350
REMARK   3
REMARK   3   TLS GROUP : 3
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B    23        B   321
REMARK   3    ORIGIN FOR THE GROUP (A):  21.1905 -31.1245  22.4056
REMARK   3    T TENSOR
REMARK   3      T11:   0.4891 T22:   0.2344
REMARK   3      T33:   0.4537 T12:   0.0939
REMARK   3      T13:  -0.1883 T23:  -0.0912
REMARK   3    L TENSOR
REMARK   3      L11:   3.3666 L22:   1.9641
REMARK   3      L33:   0.9913 L12:  -0.0347
REMARK   3      L13:   0.8826 L23:  -0.2732
REMARK   3    S TENSOR
REMARK   3      S11:   0.4701 S12:   0.5977 S13:  -0.6561
REMARK   3      S21:  -0.4409 S22:  -0.1321 S23:  -0.2024
REMARK   3      S31:   0.4002 S32:   0.2563 S33:  -0.3381
REMARK   3
REMARK   3   TLS GROUP : 4
REMARK   3    NUMBER OF COMPONENTS GROUP : 2
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B   322        B   581
REMARK   3    RESIDUE RANGE :   B     9        B    22
REMARK   3    ORIGIN FOR THE GROUP (A):  25.0555  -4.7313  30.3335
REMARK   3    T TENSOR
REMARK   3      T11:   0.1085 T22:   0.1740
REMARK   3      T33:   0.1490 T12:  -0.0015
REMARK   3      T13:  -0.0467 T23:   0.0855
REMARK   3    L TENSOR
REMARK   3      L11:   3.3399 L22:   2.9473
REMARK   3      L33:   0.9328 L12:  -0.9675
REMARK   3      L13:   1.3173 L23:  -0.3834
REMARK   3    S TENSOR
REMARK   3      S11:   0.0643 S12:   0.3858 S13:   0.2641
REMARK   3      S21:  -0.0715 S22:  -0.1729 S23:  -0.4809
REMARK   3      S31:   0.0526 S32:   0.1654 S33:   0.1087
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : BABINET MODEL WITH MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.40
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE
REMARK   3  RIDING POSITIONS
REMARK   4
REMARK   4 2HU8 COMPLIES WITH FORMAT V. 2.3, 09-JULY-1998
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 31-JUL-2006.
REMARK 100 THE RCSB ID CODE IS RCSB038767.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 21-OCT-2005
REMARK 200  TEMPERATURE           (KELVIN) : 100.0
REMARK 200  PH                             : 8.00
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : N
REMARK 200  RADIATION SOURCE               : ROTATING ANODE
REMARK 200  BEAMLINE                       : NULL
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU RU200
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418
REMARK 200  MONOCHROMATOR                  : GRAPHITE
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU RAXIS IIC
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XSCALE
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 45009
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.400
REMARK 200  RESOLUTION RANGE LOW       (A) : 19.960
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.1
REMARK 200  DATA REDUNDANCY                : NULL
REMARK 200  R MERGE                    (I) : 0.13700
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 11.6800
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.40
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.50
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.9
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 0.38000
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 4.460
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 45.51
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.26
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 20MM TRIS/HCL, 20% ETHANOL, PH 8.0,
REMARK 280  VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   1/2-X,-Y,1/2+Z
REMARK 290       3555   -X,1/2+Y,1/2-Z
REMARK 290       4555   1/2+X,1/2-Y,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       32.40500
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       84.62750
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       51.96100
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       84.62750
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       32.40500
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       51.96100
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT
REMARK 300 WHICH CONSISTS OF 2 CHAIN(S). SEE REMARK 350 FOR
REMARK 300 INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S).
REMARK 350
REMARK 350 GENERATING THE BIOMOLECULE
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     ARG A     2
REMARK 465     ILE A     3
REMARK 465     ILE A     4
REMARK 465     MET A     5
REMARK 465     PRO A     6
REMARK 465     VAL A     7
REMARK 465     MET B     1
REMARK 465     ARG B     2
REMARK 465     ILE B     3
REMARK 465     ILE B     4
REMARK 465     MET B     5
REMARK 465     PRO B     6
REMARK 465     VAL B     7
REMARK 465     GLU B     8
REMARK 465     ARG B   582
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS(M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     ARG A  11    CD    NE    CZ    NH1   NH2
REMARK 470     LYS A  85    NZ
REMARK 470     ARG A 188    CG    CD    NE    CZ    NH1   NH2
REMARK 470     LYS A 207    CE    NZ
REMARK 470     ARG A 226    NE    CZ    NH1   NH2
REMARK 470     ARG A 328    CG    CD    NE    CZ    NH1   NH2
REMARK 470     ARG A 428    NE    CZ    NH1   NH2
REMARK 470     ARG A 501    CZ    NH1   NH2
REMARK 470     GLU A 580    CD    OE1   OE2
REMARK 470     ARG A 582    CG    CD    NE    CZ    NH1   NH2
REMARK 470     ARG B  11    CG    CD    NE    CZ    NH1   NH2
REMARK 470     ARG B  14    O     CZ    NH1   NH2
REMARK 470     ASP B  34    CG    OD1   OD2
REMARK 470     LYS B  35    CD    CE    NZ
REMARK 470     GLU B  43    CD    OE1   OE2
REMARK 470     ASP B  52    CG    OD1   OD2
REMARK 470     GLU B  55    CG    CD    OE1   OE2
REMARK 470     LYS B  58    CE    NZ
REMARK 470     ARG B  61    CD    NE    CZ    NH1   NH2
REMARK 470     HIS B  71    CG    ND1   CD2   CE1   NE2
REMARK 470     LYS B  94    CG    CD    CE    NZ
REMARK 470     ARG B  99    CG    CD    NE    CZ    NH1   NH2
REMARK 470     GLU B 102    CG    CD    OE1   OE2
REMARK 470     GLU B 122    CG    CD    OE1   OE2
REMARK 470     ARG B 188    CG    CD    NE    CZ    NH1   NH2
REMARK 470     LYS B 207    CG    CD    CE    NZ
REMARK 470     ARG B 216    CG    CD    NE    CZ    NH1   NH2
REMARK 470     ARG B 226    CG    CD    NE    CZ    NH1   NH2
REMARK 470     GLU B 234    CD    OE1   OE2
REMARK 470     LYS B 238    CG    CD    CE    NZ
REMARK 470     SER B 242    OG
REMARK 470     LYS B 294    CD    CE    NZ
REMARK 470     ILE B 308    CD1
REMARK 470     GLU B 315    CG    CD    OE1   OE2
REMARK 470     GLU B 324    CG    CD    OE1   OE2
REMARK 470     ARG B 327    CD    NE    CZ    NH1   NH2
REMARK 470     ARG B 328    CG    CD    NE    CZ    NH1   NH2
REMARK 470     GLU B 352    CG    CD    OE1   OE2
REMARK 470     ARG B 511    CD    NE    CZ    NH1   NH2
REMARK 470     ARG B 581    CG    CD    NE    CZ    NH1   NH2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    MET A 394   SD    MET A 394   CE    -0.079
REMARK 500    MET A 450   SD    MET A 450   CE    -0.060
REMARK 500    MET A 477   SD    MET A 477   CE    -0.038
REMARK 500    MET A 536   SD    MET A 536   CE    -0.084
REMARK 500    ILE A 567   CG1   ILE A 567   CD1   -0.053
REMARK 500    ARG A 581   CA    ARG A 581   C     -0.040
REMARK 500    LYS B 566   CD    LYS B 566   CE    -0.059
REMARK 500    LYS B 566   CE    LYS B 566   NZ    -0.153
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    GLY A  73   N   -  CA  -  C   ANGL. DEV. =  8.0 DEGREES
REMARK 500    GLY B  73   N   -  CA  -  C   ANGL. DEV. =  7.1 DEGREES
REMARK 500    LYS B 566   CD  -  CE  -  NZ  ANGL. DEV. = 12.8 DEGREES
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ALA A 445     -111.31     60.75
REMARK 500    ALA B 445     -111.82     62.50
REMARK 525
REMARK 525 SOLVENT
REMARK 525 THE FOLLOWING SOLVENT MOLECULES LIE FARTHER THAN EXPECTED
REMARK 525 FROM THE PROTEIN OR NUCLEIC ACID MOLECULE AND MAY BE
REMARK 525 ASSOCIATED WITH A SYMMETRY RELATED MOLECULE (M=MODEL
REMARK 525 NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH   185        DISTANCE =  6.02 ANGSTROMS
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 2HU5   RELATED DB: PDB
REMARK 900 RELATED ID: 2HU7   RELATED DB: PDB
DBREF  2HU8 A    1   582  UNP    Q9YBQ2   APEH_AERPE       1    582
DBREF  2HU8 B    1   582  UNP    Q9YBQ2   APEH_AERPE       1    582
SEQADV 2HU8 ALA A  445  UNP  Q9YBQ2    SER   445 ENGINEERED
SEQADV 2HU8 ALA B  445  UNP  Q9YBQ2    SER   445 ENGINEERED
SEQRES   1 A  582  MET ARG ILE ILE MET PRO VAL GLU PHE SER ARG ILE VAL
SEQRES   2 A  582  ARG ASP VAL GLU ARG LEU ILE ALA VAL GLU LYS TYR SER
SEQRES   3 A  582  LEU GLN GLY VAL VAL ASP GLY ASP LYS LEU LEU VAL VAL
SEQRES   4 A  582  GLY PHE SER GLU GLY SER VAL ASN ALA TYR LEU TYR ASP
SEQRES   5 A  582  GLY GLY GLU THR VAL LYS LEU ASN ARG GLU PRO ILE ASN
SEQRES   6 A  582  SER VAL LEU ASP PRO HIS TYR GLY VAL GLY ARG VAL ILE
SEQRES   7 A  582  LEU VAL ARG ASP VAL SER LYS GLY ALA GLU GLN HIS ALA
SEQRES   8 A  582  LEU PHE LYS VAL ASN THR SER ARG PRO GLY GLU GLU GLN
SEQRES   9 A  582  ARG LEU GLU ALA VAL LYS PRO MET ARG ILE LEU SER GLY
SEQRES  10 A  582  VAL ASP THR GLY GLU ALA VAL VAL PHE THR GLY ALA THR
SEQRES  11 A  582  GLU ASP ARG VAL ALA LEU TYR ALA LEU ASP GLY GLY GLY
SEQRES  12 A  582  LEU ARG GLU LEU ALA ARG LEU PRO GLY PHE GLY PHE VAL
SEQRES  13 A  582  SER ASP ILE ARG GLY ASP LEU ILE ALA GLY LEU GLY PHE
SEQRES  14 A  582  PHE GLY GLY GLY ARG VAL SER LEU PHE THR SER ASN LEU
SEQRES  15 A  582  SER SER GLY GLY LEU ARG VAL PHE ASP SER GLY GLU GLY
SEQRES  16 A  582  SER PHE SER SER ALA SER ILE SER PRO GLY MET LYS VAL
SEQRES  17 A  582  THR ALA GLY LEU GLU THR ALA ARG GLU ALA ARG LEU VAL
SEQRES  18 A  582  THR VAL ASP PRO ARG ASP GLY SER VAL GLU ASP LEU GLU
SEQRES  19 A  582  LEU PRO SER LYS ASP PHE SER SER TYR ARG PRO THR ALA
SEQRES  20 A  582  ILE THR TRP LEU GLY TYR LEU PRO ASP GLY ARG LEU ALA
SEQRES  21 A  582  VAL VAL ALA ARG ARG GLU GLY ARG SER ALA VAL PHE ILE
SEQRES  22 A  582  ASP GLY GLU ARG VAL GLU ALA PRO GLN GLY ASN HIS GLY
SEQRES  23 A  582  ARG VAL VAL LEU TRP ARG GLY LYS LEU VAL THR SER HIS
SEQRES  24 A  582  THR SER LEU SER THR PRO PRO ARG ILE VAL SER LEU PRO
SEQRES  25 A  582  SER GLY GLU PRO LEU LEU GLU GLY GLY LEU PRO GLU ASP
SEQRES  26 A  582  LEU ARG ARG SER ILE ALA GLY SER ARG LEU VAL TRP VAL
SEQRES  27 A  582  GLU SER PHE ASP GLY SER ARG VAL PRO THR TYR VAL LEU
SEQRES  28 A  582  GLU SER GLY ARG ALA PRO THR PRO GLY PRO THR VAL VAL
SEQRES  29 A  582  LEU VAL HIS GLY GLY PRO PHE ALA GLU ASP SER ASP SER
SEQRES  30 A  582  TRP ASP THR PHE ALA ALA SER LEU ALA ALA ALA GLY PHE
SEQRES  31 A  582  HIS VAL VAL MET PRO ASN TYR ARG GLY SER THR GLY TYR
SEQRES  32 A  582  GLY GLU GLU TRP ARG LEU LYS ILE ILE GLY ASP PRO CYS
SEQRES  33 A  582  GLY GLY GLU LEU GLU ASP VAL SER ALA ALA ALA ARG TRP
SEQRES  34 A  582  ALA ARG GLU SER GLY LEU ALA SER GLU LEU TYR ILE MET
SEQRES  35 A  582  GLY TYR ALA TYR GLY GLY TYR MET THR LEU CYS ALA LEU
SEQRES  36 A  582  THR MET LYS PRO GLY LEU PHE LYS ALA GLY VAL ALA GLY
SEQRES  37 A  582  ALA SER VAL VAL ASP TRP GLU GLU MET TYR GLU LEU SER
SEQRES  38 A  582  ASP ALA ALA PHE ARG ASN PHE ILE GLU GLN LEU THR GLY
SEQRES  39 A  582  GLY SER ARG GLU ILE MET ARG SER ARG SER PRO ILE ASN
SEQRES  40 A  582  HIS VAL ASP ARG ILE LYS GLU PRO LEU ALA LEU ILE HIS
SEQRES  41 A  582  PRO GLN ASN ASP SER ARG THR PRO LEU LYS PRO LEU LEU
SEQRES  42 A  582  ARG LEU MET GLY GLU LEU LEU ALA ARG GLY LYS THR PHE
SEQRES  43 A  582  GLU ALA HIS ILE ILE PRO ASP ALA GLY HIS ALA ILE ASN
SEQRES  44 A  582  THR MET GLU ASP ALA VAL LYS ILE LEU LEU PRO ALA VAL
SEQRES  45 A  582  PHE PHE LEU ALA THR GLN ARG GLU ARG ARG
SEQRES   1 B  582  MET ARG ILE ILE MET PRO VAL GLU PHE SER ARG ILE VAL
SEQRES   2 B  582  ARG ASP VAL GLU ARG LEU ILE ALA VAL GLU LYS TYR SER
SEQRES   3 B  582  LEU GLN GLY VAL VAL ASP GLY ASP LYS LEU LEU VAL VAL
SEQRES   4 B  582  GLY PHE SER GLU GLY SER VAL ASN ALA TYR LEU TYR ASP
SEQRES   5 B  582  GLY GLY GLU THR VAL LYS LEU ASN ARG GLU PRO ILE ASN
SEQRES   6 B  582  SER VAL LEU ASP PRO HIS TYR GLY VAL GLY ARG VAL ILE
SEQRES   7 B  582  LEU VAL ARG ASP VAL SER LYS GLY ALA GLU GLN HIS ALA
SEQRES   8 B  582  LEU PHE LYS VAL ASN THR SER ARG PRO GLY GLU GLU GLN
SEQRES   9 B  582  ARG LEU GLU ALA VAL LYS PRO MET ARG ILE LEU SER GLY
SEQRES  10 B  582  VAL ASP THR GLY GLU ALA VAL VAL PHE THR GLY ALA THR
SEQRES  11 B  582  GLU ASP ARG VAL ALA LEU TYR ALA LEU ASP GLY GLY GLY
SEQRES  12 B  582  LEU ARG GLU LEU ALA ARG LEU PRO GLY PHE GLY PHE VAL
SEQRES  13 B  582  SER ASP ILE ARG GLY ASP LEU ILE ALA GLY LEU GLY PHE
SEQRES  14 B  582  PHE GLY GLY GLY ARG VAL SER LEU PHE THR SER ASN LEU
SEQRES  15 B  582  SER SER GLY GLY LEU ARG VAL PHE ASP SER GLY GLU GLY
SEQRES  16 B  582  SER PHE SER SER ALA SER ILE SER PRO GLY MET LYS VAL
SEQRES  17 B  582  THR ALA GLY LEU GLU THR ALA ARG GLU ALA ARG LEU VAL
SEQRES  18 B  582  THR VAL ASP PRO ARG ASP GLY SER VAL GLU ASP LEU GLU
SEQRES  19 B  582  LEU PRO SER LYS ASP PHE SER SER TYR ARG PRO THR ALA
SEQRES  20 B  582  ILE THR TRP LEU GLY TYR LEU PRO ASP GLY ARG LEU ALA
SEQRES  21 B  582  VAL VAL ALA ARG ARG GLU GLY ARG SER ALA VAL PHE ILE
SEQRES  22 B  582  ASP GLY GLU ARG VAL GLU ALA PRO GLN GLY ASN HIS GLY
SEQRES  23 B  582  ARG VAL VAL LEU TRP ARG GLY LYS LEU VAL THR SER HIS
SEQRES  24 B  582  THR SER LEU SER THR PRO PRO ARG ILE VAL SER LEU PRO
SEQRES  25 B  582  SER GLY GLU PRO LEU LEU GLU GLY GLY LEU PRO GLU ASP
SEQRES  26 B  582  LEU ARG ARG SER ILE ALA GLY SER ARG LEU VAL TRP VAL
SEQRES  27 B  582  GLU SER PHE ASP GLY SER ARG VAL PRO THR TYR VAL LEU
SEQRES  28 B  582  GLU SER GLY ARG ALA PRO THR PRO GLY PRO THR VAL VAL
SEQRES  29 B  582  LEU VAL HIS GLY GLY PRO PHE ALA GLU ASP SER ASP SER
SEQRES  30 B  582  TRP ASP THR PHE ALA ALA SER LEU ALA ALA ALA GLY PHE
SEQRES  31 B  582  HIS VAL VAL MET PRO ASN TYR ARG GLY SER THR GLY TYR
SEQRES  32 B  582  GLY GLU GLU TRP ARG LEU LYS ILE ILE GLY ASP PRO CYS
SEQRES  33 B  582  GLY GLY GLU LEU GLU ASP VAL SER ALA ALA ALA ARG TRP
SEQRES  34 B  582  ALA ARG GLU SER GLY LEU ALA SER GLU LEU TYR ILE MET
SEQRES  35 B  582  GLY TYR ALA TYR GLY GLY TYR MET THR LEU CYS ALA LEU
SEQRES  36 B  582  THR MET LYS PRO GLY LEU PHE LYS ALA GLY VAL ALA GLY
SEQRES  37 B  582  ALA SER VAL VAL ASP TRP GLU GLU MET TYR GLU LEU SER
SEQRES  38 B  582  ASP ALA ALA PHE ARG ASN PHE ILE GLU GLN LEU THR GLY
SEQRES  39 B  582  GLY SER ARG GLU ILE MET ARG SER ARG SER PRO ILE ASN
SEQRES  40 B  582  HIS VAL ASP ARG ILE LYS GLU PRO LEU ALA LEU ILE HIS
SEQRES  41 B  582  PRO GLN ASN ASP SER ARG THR PRO LEU LYS PRO LEU LEU
SEQRES  42 B  582  ARG LEU MET GLY GLU LEU LEU ALA ARG GLY LYS THR PHE
SEQRES  43 B  582  GLU ALA HIS ILE ILE PRO ASP ALA GLY HIS ALA ILE ASN
SEQRES  44 B  582  THR MET GLU ASP ALA VAL LYS ILE LEU LEU PRO ALA VAL
SEQRES  45 B  582  PHE PHE LEU ALA THR GLN ARG GLU ARG ARG
HET    BE2   1001       9
HET    GLY   1002       4
HET    PHE   1003      12
HET    BE2   2001       9
HET    GLY   2002       4
HET    PHE   2003      12
HETNAM     BE2 2-AMINOBENZOIC ACID
HETNAM     GLY GLYCINE
HETNAM     PHE PHENYLALANINE
FORMUL   3  BE2    2(C7 H7 N1 O2)
FORMUL   3  GLY    2(C2 H5 N1 O2)
FORMUL   3  PHE    2(C9 H11 N1 O2)
FORMUL   5  HOH   *223(H2 O1)
HELIX    1   1 GLU A    8  VAL A   22  1                                  15
HELIX    2   2 LYS A  238  ARG A  244  1                                   7
HELIX    3   3 PRO A  323  SER A  329  1                                   7
HELIX    4   4 ASP A  379  ALA A  388  1                                  10
HELIX    5   5 GLY A  404  LYS A  410  1                                   7
HELIX    6   6 GLY A  417  SER A  433  1                                  17
HELIX    7   7 ALA A  445  LYS A  458  1                                  14
HELIX    8   8 ASP A  473  SER A  481  1                                   9
HELIX    9   9 ASP A  482  GLY A  494  1                                  13
HELIX   10  10 SER A  496  ARG A  503  1                                   8
HELIX   11  11 SER A  504  ILE A  512  5                                   9
HELIX   12  12 PRO A  528  ARG A  542  1                                  15
HELIX   13  13 THR A  560  GLU A  580  1                                  21
HELIX   14  14 PHE B    9  VAL B   22  1                                  14
HELIX   15  15 PHE B  240  ARG B  244  5                                   5
HELIX   16  16 PRO B  323  SER B  329  1                                   7
HELIX   17  17 ASP B  379  ALA B  388  1                                  10
HELIX   18  18 GLY B  404  LYS B  410  1                                   7
HELIX   19  19 GLY B  417  SER B  433  1                                  17
HELIX   20  20 ALA B  445  LYS B  458  1                                  14
HELIX   21  21 ASP B  473  LEU B  480  1                                   8
HELIX   22  22 ASP B  482  GLY B  494  1                                  13
HELIX   23  23 SER B  496  ARG B  503  1                                   8
HELIX   24  24 SER B  504  ILE B  512  5                                   9
HELIX   25  25 PRO B  528  ARG B  542  1                                  15
HELIX   26  26 THR B  560  GLU B  580  1                                  21
SHEET    1   A 4 LYS A  24  VAL A  31  0
SHEET    2   A 4 LYS A  35  SER A  42 -1  O  LYS A  35   N  VAL A  31
SHEET    3   A 4 SER A  45  TYR A  51 -1  O  SER A  45   N  SER A  42
SHEET    4   A 4 VAL A  57  LYS A  58 -1  O  VAL A  57   N  LEU A  50
SHEET    1   B 4 SER A  66  VAL A  67  0
SHEET    2   B 4 ARG A  76  ASP A  82 -1  O  VAL A  80   N  SER A  66
SHEET    3   B 4 HIS A  90  ASN A  96 -1  O  PHE A  93   N  LEU A  79
SHEET    4   B 4 GLN A 104  ARG A 105 -1  O  GLN A 104   N  LYS A  94
SHEET    1   C 5 ASP A  69  PRO A  70  0
SHEET    2   C 5 ARG A 113  ASP A 119  1  O  ASP A 119   N  ASP A  69
SHEET    3   C 5 VAL A 124  ALA A 129 -1  O  VAL A 125   N  VAL A 118
SHEET    4   C 5 VAL A 134  ASP A 140 -1  O  TYR A 137   N  PHE A 126
SHEET    5   C 5 GLY A 143  LEU A 150 -1  O  ALA A 148   N  LEU A 136
SHEET    1   D 4 GLY A 154  ARG A 160  0
SHEET    2   D 4 LEU A 163  GLY A 171 -1  O  LEU A 167   N  PHE A 155
SHEET    3   D 4 ARG A 174  ASN A 181 -1  O  SER A 176   N  GLY A 168
SHEET    4   D 4 GLY A 185  PHE A 190 -1  O  PHE A 190   N  LEU A 177
SHEET    1   E 4 GLY A 195  ILE A 202  0
SHEET    2   E 4 VAL A 208  THR A 214 -1  O  GLU A 213   N  SER A 196
SHEET    3   E 4 ALA A 218  VAL A 223 -1  O  VAL A 223   N  VAL A 208
SHEET    4   E 4 VAL A 230  ASP A 232 -1  O  GLU A 231   N  THR A 222
SHEET    1   F 4 ALA A 247  TYR A 253  0
SHEET    2   F 4 LEU A 259  ARG A 265 -1  O  VAL A 262   N  TRP A 250
SHEET    3   F 4 ARG A 268  ILE A 273 -1  O  PHE A 272   N  VAL A 261
SHEET    4   F 4 GLU A 276  VAL A 278 -1  O  GLU A 276   N  ILE A 273
SHEET    1   G 4 ASN A 284  TRP A 291  0
SHEET    2   G 4 LYS A 294  SER A 301 -1  O  LYS A 294   N  TRP A 291
SHEET    3   G 4 THR A 304  LEU A 311 -1  O  VAL A 309   N  THR A 297
SHEET    4   G 4 PRO A 316  LEU A 318 -1  O  LEU A 318   N  ILE A 308
SHEET    1   H16 ILE A 330  GLU A 339  0
SHEET    2   H16 ARG A 345  SER A 353 -1  O  THR A 348   N  VAL A 336
SHEET    3   H16 HIS A 391  PRO A 395 -1  O  VAL A 392   N  LEU A 351
SHEET    4   H16 GLY A 360  VAL A 366  1  N  PRO A 361   O  HIS A 391
SHEET    5   H16 ALA A 436  TYR A 444  1  O  TYR A 440   N  VAL A 364
SHEET    6   H16 ALA A 464  GLY A 468  1  O  VAL A 466   N  ILE A 441
SHEET    7   H16 LEU A 516  PRO A 521  1  O  ALA A 517   N  ALA A 467
SHEET    8   H16 PHE A 546  ILE A 551  1  O  GLU A 547   N  LEU A 518
SHEET    9   H16 PHE B 546  ILE B 551 -1  O  ILE B 550   N  ALA A 548
SHEET   10   H16 LEU B 516  PRO B 521  1  N  LEU B 518   O  GLU B 547
SHEET   11   H16 ALA B 464  GLY B 468  1  N  ALA B 467   O  ALA B 517
SHEET   12   H16 ALA B 436  TYR B 444  1  N  GLY B 443   O  GLY B 468
SHEET   13   H16 GLY B 360  VAL B 366  1  N  VAL B 364   O  TYR B 440
SHEET   14   H16 HIS B 391  PRO B 395  1  O  HIS B 391   N  PRO B 361
SHEET   15   H16 ARG B 345  SER B 353 -1  N  LEU B 351   O  VAL B 392
SHEET   16   H16 ILE B 330  GLU B 339 -1  N  VAL B 336   O  THR B 348
SHEET    1   I 4 LYS B  24  VAL B  31  0
SHEET    2   I 4 LYS B  35  SER B  42 -1  O  PHE B  41   N  LYS B  24
SHEET    3   I 4 SER B  45  TYR B  51 -1  O  ASN B  47   N  GLY B  40
SHEET    4   I 4 VAL B  57  LYS B  58 -1  O  VAL B  57   N  LEU B  50
SHEET    1   J 4 SER B  66  VAL B  67  0
SHEET    2   J 4 ARG B  76  ASP B  82 -1  O  VAL B  80   N  SER B  66
SHEET    3   J 4 HIS B  90  ASN B  96 -1  O  PHE B  93   N  LEU B  79
SHEET    4   J 4 GLN B 104  ARG B 105 -1  O  GLN B 104   N  LYS B  94
SHEET    1   K 5 ASP B  69  PRO B  70  0
SHEET    2   K 5 ARG B 113  ASP B 119  1  O  ASP B 119   N  ASP B  69
SHEET    3   K 5 VAL B 124  ALA B 129 -1  O  VAL B 125   N  VAL B 118
SHEET    4   K 5 VAL B 134  ASP B 140 -1  O  TYR B 137   N  PHE B 126
SHEET    5   K 5 GLY B 143  LEU B 150 -1  O  ALA B 148   N  LEU B 136
SHEET    1   L 4 GLY B 154  ARG B 160  0
SHEET    2   L 4 LEU B 163  GLY B 171 -1  O  LEU B 167   N  PHE B 155
SHEET    3   L 4 ARG B 174  ASN B 181 -1  O  SER B 176   N  GLY B 168
SHEET    4   L 4 GLY B 185  PHE B 190 -1  O  PHE B 190   N  LEU B 177
SHEET    1   M 4 GLY B 195  ILE B 202  0
SHEET    2   M 4 VAL B 208  THR B 214 -1  O  GLU B 213   N  SER B 196
SHEET    3   M 4 GLU B 217  VAL B 223 -1  O  ARG B 219   N  LEU B 212
SHEET    4   M 4 VAL B 230  ASP B 232 -1  O  GLU B 231   N  THR B 222
SHEET    1   N 4 ALA B 247  TYR B 253  0
SHEET    2   N 4 LEU B 259  ARG B 265 -1  O  VAL B 262   N  TRP B 250
SHEET    3   N 4 ARG B 268  ILE B 273 -1  O  PHE B 272   N  VAL B 261
SHEET    4   N 4 GLU B 276  VAL B 278 -1  O  GLU B 276   N  ILE B 273
SHEET    1   O 4 ASN B 284  TRP B 291  0
SHEET    2   O 4 LYS B 294  SER B 301 -1  O  LYS B 294   N  TRP B 291
SHEET    3   O 4 THR B 304  LEU B 311 -1  O  VAL B 309   N  THR B 297
SHEET    4   O 4 PRO B 316  LEU B 318 -1  O  LEU B 318   N  ILE B 308
LINK         C   BE2  1001                 N   GLY  1002
LINK         C   GLY  1002                 N   PHE  1003
LINK         C   BE2  2001                 N   GLY  2002
LINK         C   GLY  2002                 N   PHE  2003
CISPEP   1 LEU A  311    PRO A  312          0         8.89
CISPEP   2 THR A  358    PRO A  359          0         3.24
CISPEP   3 GLY A  369    PRO A  370          0         7.17
CISPEP   4 GLY B   53    GLY B   54          0         1.38
CISPEP   5 LEU B  311    PRO B  312          0         7.21
CISPEP   6 THR B  358    PRO B  359          0         5.64
CISPEP   7 GLY B  369    PRO B  370          0         5.99
CRYST1   64.810  103.922  169.255  90.00  90.00  90.00 P 21 21 21    8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.015430  0.000000  0.000000        0.00000
SCALE2      0.000000  0.009623  0.000000        0.00000
SCALE3      0.000000  0.000000  0.005908        0.00000
TER    4364      ARG A 582
TER    8619      ARG B 581
MASTER      519    0    6   26   74    0    0    6 8890    2   24   90
END