longtext: 2ORY-pdb

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HEADER    HYDROLASE                               05-FEB-07   2ORY
TITLE     CRYSTAL STRUCTURE OF M37 LIPASE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: LIPASE;
COMPND   3 CHAIN: A, B;
COMPND   4 EC: 3.1.1.3;
COMPND   5 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: PHOTOBACTERIUM SP. M37;
SOURCE   3 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   4 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   5 EXPRESSION_SYSTEM_PLASMID: PET22B
KEYWDS    ALPHA/BETA HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    S.K.JUNG,D.G.JEONG,S.J.KIM
REVDAT   1   29-JAN-08 2ORY    0
JRNL        AUTH   S.K.JUNG,D.G.JEONG,M.S.LEE,J.K.LEE,H.K.KIM,S.E.RYU,
JRNL        AUTH 2 B.C.PARK,J.H.KIM,S.J.KIM
JRNL        TITL   STRUCTURAL BASIS FOR THE COLD ADAPTATION OF
JRNL        TITL 2 PSYCHROPHILIC M37 LIPASE FROM PHOTOBACTERIUM
JRNL        TITL 3 LIPOLYTICUM
JRNL        REF    PROTEINS                                   2008
JRNL        REFN   ASTM PSFGEY  US ESSN 1097-0134
REMARK   1
REMARK   2
REMARK   2 RESOLUTION. 2.20 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : CNS 0.9
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,
REMARK   3               : READ,RICE,SIMONSON,WARREN
REMARK   3
REMARK   3  REFINEMENT TARGET : ENGH & HUBER
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.20
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 50.00
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 98.8
REMARK   3   NUMBER OF REFLECTIONS             : 34410
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE            (WORKING SET) : 0.204
REMARK   3   FREE R VALUE                     : 0.240
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : NULL
REMARK   3   FREE R VALUE TEST SET COUNT      : 1711
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL
REMARK   3   BIN R VALUE           (WORKING SET) : NULL
REMARK   3   BIN FREE R VALUE                    : NULL
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 5363
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 0
REMARK   3   SOLVENT ATOMS            : 182
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL
REMARK   3   ESD FROM SIGMAA              (A) : NULL
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL
REMARK   3
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.
REMARK   3   BOND LENGTHS                 (A) : 0.010
REMARK   3   BOND ANGLES            (DEGREES) : 1.42
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 23.40
REMARK   3   IMPROPER ANGLES        (DEGREES) : 0.89
REMARK   3
REMARK   3  ISOTROPIC THERMAL MODEL : ISOTROPHIC
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELING.
REMARK   3   METHOD USED : NULL
REMARK   3   KSOL        : NULL
REMARK   3   BSOL        : NULL
REMARK   3
REMARK   3  NCS MODEL : NULL
REMARK   3
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  PARAMETER FILE  1  : NULL
REMARK   3  TOPOLOGY FILE  1   : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 2ORY COMPLIES WITH FORMAT V. 3.1, 01-AUG-2007
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ.
REMARK 100 THE RCSB ID CODE IS RCSB041511.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 06-JUL-2005
REMARK 200  TEMPERATURE           (KELVIN) : 93.0
REMARK 200  PH                             : 5.50
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : PAL/PLS
REMARK 200  BEAMLINE                       : 4A
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.0
REMARK 200  MONOCHROMATOR                  : MIRRORS
REMARK 200  OPTICS                         : MIRRORS
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 210
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MOSFLM
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 34410
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.200
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.8
REMARK 200  DATA REDUNDANCY                : 6.300
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : 0.05200
REMARK 200   FOR THE DATA SET  : 11.7000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.20
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.32
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.30
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : 0.21500
REMARK 200   FOR SHELL         : 3.600
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MIR
REMARK 200 SOFTWARE USED: SOLVE
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 42.87
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.15
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2M AMMONIUM ACETATE, 23-25% PEG
REMARK 280  3350, PH 5.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   1/2-X,-Y,1/2+Z
REMARK 290       3555   -X,1/2+Y,1/2-Z
REMARK 290       4555   1/2+X,1/2-Y,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       35.34700
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       49.85000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       47.55100
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       49.85000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       35.34700
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       47.55100
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMER
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMER
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C  SSEQI
REMARK 465     MET A     1
REMARK 465     GLY A    24
REMARK 465     SER A    25
REMARK 465     ALA A    26
REMARK 465     LYS A    27
REMARK 465     HIS A   341
REMARK 465     HIS A   342
REMARK 465     HIS A   343
REMARK 465     HIS A   344
REMARK 465     HIS A   345
REMARK 465     HIS A   346
REMARK 465     MET B     1
REMARK 465     SER B     2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    LEU A  41       -7.27    -58.00
REMARK 500    LEU A 101      -62.40   -100.87
REMARK 500    MET A 106        8.24    -65.12
REMARK 500    GLU A 148      126.11    -39.93
REMARK 500    SER A 174     -130.51     65.05
REMARK 500    VAL A 193      -55.89   -126.53
REMARK 500    LYS A 248        3.71    -69.10
REMARK 500    SER A 256     -149.40   -121.14
REMARK 500    PRO A 327       74.91    -67.97
REMARK 500    GLU A 333     -127.16     51.54
REMARK 500    ALA A 337      -97.29      3.04
REMARK 500    GLU B  82      -78.31    -56.34
REMARK 500    ALA B 116      148.28   -175.17
REMARK 500    HIS B 144       -2.95     80.68
REMARK 500    GLU B 148      134.02    -33.44
REMARK 500    SER B 174     -128.78     66.47
REMARK 500    SER B 256     -158.90   -157.94
REMARK 500    GLU B 257      104.36    -44.64
REMARK 500    GLN B 258      -25.33     58.95
REMARK 500    GLU B 333      173.20     68.78
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        RMS     TYPE
REMARK 500    TYR B 316         0.08    SIDE_CHAIN
REMARK 500
REMARK 500 REMARK: NULL
DBREF  2ORY A    1   340  UNP    Q5DRN8   Q5DRN8_9GAMM     1    340
DBREF  2ORY B    1   340  UNP    Q5DRN8   Q5DRN8_9GAMM     1    340
SEQADV 2ORY HIS A  341  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS A  342  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS A  343  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS A  344  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS A  345  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS A  346  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS B  341  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS B  342  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS B  343  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS B  344  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS B  345  UNP  Q5DRN8              EXPRESSION TAG
SEQADV 2ORY HIS B  346  UNP  Q5DRN8              EXPRESSION TAG
SEQRES   1 A  346  MET SER TYR THR LYS GLU GLN LEU MET LEU ALA PHE SER
SEQRES   2 A  346  TYR MET SER TYR TYR GLY ILE THR HIS THR GLY SER ALA
SEQRES   3 A  346  LYS LYS ASN ALA GLU LEU ILE LEU LYS LYS MET LYS GLU
SEQRES   4 A  346  ALA LEU LYS THR TRP LYS PRO PHE GLN GLU ASP ASP TRP
SEQRES   5 A  346  GLU VAL VAL TRP GLY PRO ALA VAL TYR THR MET PRO PHE
SEQRES   6 A  346  THR ILE PHE ASN ASP ALA MET MET TYR VAL ILE GLN LYS
SEQRES   7 A  346  LYS GLY ALA GLU GLY GLU TYR VAL ILE ALA ILE ARG GLY
SEQRES   8 A  346  THR ASN PRO VAL SER ILE SER ASP TRP LEU PHE ASN ASP
SEQRES   9 A  346  PHE MET VAL SER ALA MET LYS LYS TRP PRO TYR ALA SER
SEQRES  10 A  346  VAL GLU GLY ARG ILE LEU LYS ILE SER GLU SER THR SER
SEQRES  11 A  346  TYR GLY LEU LYS THR LEU GLN LYS LEU LYS PRO LYS SER
SEQRES  12 A  346  HIS ILE PRO GLY GLU ASN LYS THR ILE LEU GLN PHE LEU
SEQRES  13 A  346  ASN GLU LYS ILE GLY PRO GLU GLY LYS ALA LYS ILE CYS
SEQRES  14 A  346  VAL THR GLY HIS SER LYS GLY GLY ALA LEU SER SER THR
SEQRES  15 A  346  LEU ALA LEU TRP LEU LYS ASP ILE GLN GLY VAL LYS LEU
SEQRES  16 A  346  SER GLN ASN ILE ASP ILE SER THR ILE PRO PHE ALA GLY
SEQRES  17 A  346  PRO THR ALA GLY ASN ALA ASP PHE ALA ASP TYR PHE ASP
SEQRES  18 A  346  ASP CYS LEU GLY ASP GLN CYS THR ARG ILE ALA ASN SER
SEQRES  19 A  346  LEU ASP ILE VAL PRO TYR ALA TRP ASN THR ASN SER LEU
SEQRES  20 A  346  LYS LYS LEU LYS SER ILE TYR ILE SER GLU GLN ALA SER
SEQRES  21 A  346  VAL LYS PRO LEU LEU TYR GLN ARG ALA LEU ILE ARG ALA
SEQRES  22 A  346  MET ILE ALA GLU THR LYS GLY LYS LYS TYR LYS GLN ILE
SEQRES  23 A  346  LYS ALA GLU THR PRO PRO LEU GLU GLY ASN ILE ASN PRO
SEQRES  24 A  346  ILE LEU ILE GLU TYR LEU VAL GLN ALA ALA TYR GLN HIS
SEQRES  25 A  346  VAL VAL GLY TYR PRO GLU LEU MET GLY MET MET ASP ASP
SEQRES  26 A  346  ILE PRO LEU THR ASP ILE PHE GLU ASP ALA ILE ALA GLY
SEQRES  27 A  346  LEU LEU HIS HIS HIS HIS HIS HIS
SEQRES   1 B  346  MET SER TYR THR LYS GLU GLN LEU MET LEU ALA PHE SER
SEQRES   2 B  346  TYR MET SER TYR TYR GLY ILE THR HIS THR GLY SER ALA
SEQRES   3 B  346  LYS LYS ASN ALA GLU LEU ILE LEU LYS LYS MET LYS GLU
SEQRES   4 B  346  ALA LEU LYS THR TRP LYS PRO PHE GLN GLU ASP ASP TRP
SEQRES   5 B  346  GLU VAL VAL TRP GLY PRO ALA VAL TYR THR MET PRO PHE
SEQRES   6 B  346  THR ILE PHE ASN ASP ALA MET MET TYR VAL ILE GLN LYS
SEQRES   7 B  346  LYS GLY ALA GLU GLY GLU TYR VAL ILE ALA ILE ARG GLY
SEQRES   8 B  346  THR ASN PRO VAL SER ILE SER ASP TRP LEU PHE ASN ASP
SEQRES   9 B  346  PHE MET VAL SER ALA MET LYS LYS TRP PRO TYR ALA SER
SEQRES  10 B  346  VAL GLU GLY ARG ILE LEU LYS ILE SER GLU SER THR SER
SEQRES  11 B  346  TYR GLY LEU LYS THR LEU GLN LYS LEU LYS PRO LYS SER
SEQRES  12 B  346  HIS ILE PRO GLY GLU ASN LYS THR ILE LEU GLN PHE LEU
SEQRES  13 B  346  ASN GLU LYS ILE GLY PRO GLU GLY LYS ALA LYS ILE CYS
SEQRES  14 B  346  VAL THR GLY HIS SER LYS GLY GLY ALA LEU SER SER THR
SEQRES  15 B  346  LEU ALA LEU TRP LEU LYS ASP ILE GLN GLY VAL LYS LEU
SEQRES  16 B  346  SER GLN ASN ILE ASP ILE SER THR ILE PRO PHE ALA GLY
SEQRES  17 B  346  PRO THR ALA GLY ASN ALA ASP PHE ALA ASP TYR PHE ASP
SEQRES  18 B  346  ASP CYS LEU GLY ASP GLN CYS THR ARG ILE ALA ASN SER
SEQRES  19 B  346  LEU ASP ILE VAL PRO TYR ALA TRP ASN THR ASN SER LEU
SEQRES  20 B  346  LYS LYS LEU LYS SER ILE TYR ILE SER GLU GLN ALA SER
SEQRES  21 B  346  VAL LYS PRO LEU LEU TYR GLN ARG ALA LEU ILE ARG ALA
SEQRES  22 B  346  MET ILE ALA GLU THR LYS GLY LYS LYS TYR LYS GLN ILE
SEQRES  23 B  346  LYS ALA GLU THR PRO PRO LEU GLU GLY ASN ILE ASN PRO
SEQRES  24 B  346  ILE LEU ILE GLU TYR LEU VAL GLN ALA ALA TYR GLN HIS
SEQRES  25 B  346  VAL VAL GLY TYR PRO GLU LEU MET GLY MET MET ASP ASP
SEQRES  26 B  346  ILE PRO LEU THR ASP ILE PHE GLU ASP ALA ILE ALA GLY
SEQRES  27 B  346  LEU LEU HIS HIS HIS HIS HIS HIS
FORMUL   3  HOH   *182(H2 O)
HELIX    1   1 THR A    4  SER A   16  1                                  13
HELIX    2   2 ASN A   29  TRP A   44  1                                  16
HELIX    3   3 LYS A   45  GLN A   48  5                                   4
HELIX    4   4 SER A   96  LEU A  101  1                                   6
HELIX    5   5 ASP A  104  SER A  108  5                                   5
HELIX    6   6 GLU A  127  LEU A  139  1                                  13
HELIX    7   7 THR A  151  GLY A  161  1                                  11
HELIX    8   8 SER A  174  ILE A  190  1                                  17
HELIX    9   9 ASN A  213  GLY A  225  1                                  13
HELIX   10  10 ASP A  226  CYS A  228  5                                   3
HELIX   11  11 ILE A  237  ALA A  241  5                                   5
HELIX   12  12 ASN A  243  LYS A  248  1                                   6
HELIX   13  13 LEU A  264  LYS A  279  1                                  16
HELIX   14  14 GLU A  303  VAL A  314  1                                  12
HELIX   15  15 VAL A  314  MET A  320  1                                   7
HELIX   16  16 PRO A  327  GLU A  333  1                                   7
HELIX   17  17 ASP A  334  ALA A  337  5                                   4
HELIX   18  18 THR B    4  SER B   16  1                                  13
HELIX   19  19 SER B   25  LYS B   28  5                                   4
HELIX   20  20 ASN B   29  TRP B   44  1                                  16
HELIX   21  21 LYS B   45  ASP B   50  5                                   6
HELIX   22  22 SER B   96  LEU B  101  1                                   6
HELIX   23  23 ASP B  104  SER B  108  5                                   5
HELIX   24  24 GLU B  127  LEU B  139  1                                  13
HELIX   25  25 THR B  151  GLY B  161  1                                  11
HELIX   26  26 SER B  174  GLN B  191  1                                  18
HELIX   27  27 ASN B  213  GLY B  225  1                                  13
HELIX   28  28 ASP B  226  CYS B  228  5                                   3
HELIX   29  29 ILE B  237  ALA B  241  5                                   5
HELIX   30  30 ASN B  243  LYS B  248  1                                   6
HELIX   31  31 LEU B  264  LYS B  279  1                                  16
HELIX   32  32 GLU B  303  VAL B  314  1                                  12
HELIX   33  33 VAL B  314  MET B  320  1                                   7
HELIX   34  34 PRO B  327  LEU B  340  1                                  14
HELIX   35  35 HIS B  341  HIS B  346  1                                   6
SHEET    1   A 5 TRP A  52  MET A  63  0
SHEET    2   A 5 THR A  66  LYS A  78 -1  O  ALA A  71   N  TYR A  61
SHEET    3   A 5 GLU A  84  ARG A  90 -1  O  ARG A  90   N  MET A  72
SHEET    4   A 5 ALA A 166  HIS A 173  1  O  LYS A 167   N  TYR A  85
SHEET    5   A 5 ILE A 199  PHE A 206  1  O  ASP A 200   N  ILE A 168
SHEET    1   B 2 MET A 110  LYS A 112  0
SHEET    2   B 2 LYS A 124  SER A 126 -1  O  ILE A 125   N  LYS A 111
SHEET    1   C 5 ASP B  51  TYR B  61  0
SHEET    2   C 5 ALA B  71  LYS B  79 -1  O  LYS B  79   N  ASP B  51
SHEET    3   C 5 GLU B  84  ARG B  90 -1  O  ALA B  88   N  TYR B  74
SHEET    4   C 5 ALA B 166  HIS B 173  1  O  THR B 171   N  ILE B  87
SHEET    5   C 5 ILE B 199  PHE B 206  1  O  ASP B 200   N  ILE B 168
SHEET    1   D 2 MET B 110  LYS B 112  0
SHEET    2   D 2 LYS B 124  SER B 126 -1  O  ILE B 125   N  LYS B 111
CISPEP   1 GLY A   57    PRO A   58          0         0.72
CISPEP   2 ALA A  241    TRP A  242          0         0.57
CISPEP   3 GLY B   57    PRO B   58          0         1.62
CISPEP   4 ALA B  241    TRP B  242          0         1.32
CRYST1   70.694   95.102   99.700  90.00  90.00  90.00 P 21 21 21    8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.014145  0.000000  0.000000        0.00000
SCALE2      0.000000  0.010515  0.000000        0.00000
SCALE3      0.000000  0.000000  0.010030        0.00000
TER    2643      LEU A 340
TER    5365      HIS B 346
MASTER      289    0    0   35   14    0    0    6 5545    2    0   54
END