longtext: 2WSL-pdb

content
HEADER    HYDROLASE                               08-SEP-09   2WSL
TITLE     AGED FORM OF HUMAN BUTYRYLCHOLINESTERASE INHIBITED BY TABUN
TITLE    2 ANALOGUE TA4
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: CHOLINESTERASE;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: BUTYRYLCHOLINESTERASE, ACYLCHOLINE ACYLHYDROLASE,
COMPND   5  CHOLINE ESTERASE II, BUTYRYLCHOLINE ESTERASE,
COMPND   6  PSEUDOCHOLINESTERASE;
COMPND   7 EC: 3.1.1.8;
COMPND   8 ENGINEERED: YES;
COMPND   9 MUTATION: YES;
COMPND  10 OTHER_DETAILS: S198 IS PHOSPHORAMIDYLATED
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE   3 ORGANISM_COMMON: HUMAN;
SOURCE   4 ORGANISM_TAXID: 9606;
SOURCE   5 EXPRESSION_SYSTEM: CRICETULUS GRISEUS;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 10029;
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: CHO-K1;
SOURCE   8 EXPRESSION_SYSTEM_CELL_LINE: OVARY CELLS;
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PGS
KEYWDS    TABUN, AGING, HYDROLASE, INHIBITION, POLYMORPHISM,
KEYWDS   2 GLYCOPROTEIN, SERINE ESTERASE, DISEASE MUTATION,
KEYWDS   3 BUTYRYLCHOLINESTERASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    E.CARLETTI,N.AURBEK,E.GILLON,M.LOIODICE,Y.NICOLET,
AUTHOR   2 J.FONTECILLA,P.MASSON,H.THIERMANN,F.NACHON,F.WOREK
REVDAT   1   15-SEP-09 2WSL    0
JRNL        AUTH   E.CARLETTI,N.AURBEK,E.GILLON,M.LOIODICE,Y.NICOLET,
JRNL        AUTH 2 J.C.FONTECILLA-CAMPS,P.MASSON,H.THIERMANN,F.NACHON,
JRNL        AUTH 3 F.WOREK
JRNL        TITL   STRUCTURE-ACTIVITY ANALYSIS OF AGING AND
JRNL        TITL 2 REACTIVATION OF HUMAN BUTYRYLCHOLINESTERASE
JRNL        TITL 3 INHIBITED BY ANALOGUES OF TABUN.
JRNL        REF    BIOCHEM.J.                    V. 421    97 2009
JRNL        REFN                   ISSN 0264-6021
JRNL        PMID   19368529
JRNL        DOI    10.1042/BJ20090091
REMARK   2
REMARK   2 RESOLUTION.    2.00 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.5.0044
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.00
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 48.87
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NONE
REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.00
REMARK   3   NUMBER OF REFLECTIONS             : 49870
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.18536
REMARK   3   R VALUE            (WORKING SET) : 0.18431
REMARK   3   FREE R VALUE                     : 0.21957
REMARK   3   FREE R VALUE TEST SET SIZE   (%) :  3.0
REMARK   3   FREE R VALUE TEST SET COUNT      : 1543
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.002
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.054
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 3603
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 100.00
REMARK   3   BIN R VALUE           (WORKING SET) : 0.234
REMARK   3   BIN FREE R VALUE SET COUNT          : 112
REMARK   3   BIN FREE R VALUE                    : 0.272
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 4263
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 157
REMARK   3   SOLVENT ATOMS            : 397
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 27.899
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.00
REMARK   3    B22 (A**2) : 0.00
REMARK   3    B33 (A**2) : 0.01
REMARK   3    B12 (A**2) : 0.00
REMARK   3    B13 (A**2) : 0.00
REMARK   3    B23 (A**2) : 0.00
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.148
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.138
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.092
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 7.254
REMARK   3
REMARK   3  CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.957
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.936
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  4560 ; 0.011 ; 0.022
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  6213 ; 1.351 ; 1.982
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   544 ; 6.209 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   210 ;34.223 ;24.095
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   721 ;15.542 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    23 ;16.084 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   681 ; 0.101 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  3487 ; 0.006 ; 0.021
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  2652 ; 0.691 ; 1.500
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  4294 ; 1.278 ; 2.000
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  1908 ; 1.786 ; 3.000
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1911 ; 2.916 ; 4.500
REMARK   3
REMARK   3  ANISOTROPIC THERMAL FACTOR RESTRAINTS.   COUNT   RMS    WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 1
REMARK   3
REMARK   3   TLS GROUP :     1
REMARK   3    NUMBER OF COMPONENTS GROUP :    1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A     1        A  2397
REMARK   3    ORIGIN FOR THE GROUP (A):  32.1619  16.9442  24.8764
REMARK   3    T TENSOR
REMARK   3      T11:   0.0137 T22:   0.0163
REMARK   3      T33:   0.0319 T12:  -0.0000
REMARK   3      T13:  -0.0144 T23:  -0.0105
REMARK   3    L TENSOR
REMARK   3      L11:   0.5499 L22:   0.4482
REMARK   3      L33:   0.7360 L12:   0.1086
REMARK   3      L13:   0.0327 L23:   0.1779
REMARK   3    S TENSOR
REMARK   3      S11:   0.0394 S12:  -0.0688 S13:  -0.0285
REMARK   3      S21:  -0.0051 S22:   0.0095 S23:  -0.0458
REMARK   3      S31:  -0.0298 S32:   0.0124 S33:  -0.0489
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : BABINET MODEL WITH MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.40
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE
REMARK   3   RIDING POSITIONS.
REMARK   4
REMARK   4 2WSL COMPLIES WITH FORMAT V. 3.20, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON  08-SEP-09.
REMARK 100 THE PDBE ID CODE IS EBI-41066.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 25-APR-08
REMARK 200  TEMPERATURE           (KELVIN) : 77
REMARK 200  PH                             : 6.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : ESRF
REMARK 200  BEAMLINE                       : ID14-4
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9765
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XSCALE
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 51418
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.00
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.00
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.0
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8
REMARK 200  DATA REDUNDANCY                : 7.3
REMARK 200  R MERGE                    (I) : 0.09
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 22.80
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.10
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.8
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.3
REMARK 200  R MERGE FOR SHELL          (I) : 0.04
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 4.35
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: XDS
REMARK 200 STARTING MODEL: PDB ENTRY 1P0I
REMARK 200
REMARK 200 REMARK: NONE
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 55.7
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.8
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M MES BUFFER PH 6.5, 2.1M
REMARK 280  AMMONIUM SULFATE
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: I 4 2 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z
REMARK 290       3555   -Y,X,Z
REMARK 290       4555   Y,-X,Z
REMARK 290       5555   -X,Y,-Z
REMARK 290       6555   X,-Y,-Z
REMARK 290       7555   Y,X,-Z
REMARK 290       8555   -Y,-X,-Z
REMARK 290       9555   X+1/2,Y+1/2,Z+1/2
REMARK 290      10555   -X+1/2,-Y+1/2,Z+1/2
REMARK 290      11555   -Y+1/2,X+1/2,Z+1/2
REMARK 290      12555   Y+1/2,-X+1/2,Z+1/2
REMARK 290      13555   -X+1/2,Y+1/2,-Z+1/2
REMARK 290      14555   X+1/2,-Y+1/2,-Z+1/2
REMARK 290      15555   Y+1/2,X+1/2,-Z+1/2
REMARK 290      16555   -Y+1/2,-X+1/2,-Z+1/2
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   9  1.000000  0.000000  0.000000       77.43000
REMARK 290   SMTRY2   9  0.000000  1.000000  0.000000       77.43000
REMARK 290   SMTRY3   9  0.000000  0.000000  1.000000       63.00500
REMARK 290   SMTRY1  10 -1.000000  0.000000  0.000000       77.43000
REMARK 290   SMTRY2  10  0.000000 -1.000000  0.000000       77.43000
REMARK 290   SMTRY3  10  0.000000  0.000000  1.000000       63.00500
REMARK 290   SMTRY1  11  0.000000 -1.000000  0.000000       77.43000
REMARK 290   SMTRY2  11  1.000000  0.000000  0.000000       77.43000
REMARK 290   SMTRY3  11  0.000000  0.000000  1.000000       63.00500
REMARK 290   SMTRY1  12  0.000000  1.000000  0.000000       77.43000
REMARK 290   SMTRY2  12 -1.000000  0.000000  0.000000       77.43000
REMARK 290   SMTRY3  12  0.000000  0.000000  1.000000       63.00500
REMARK 290   SMTRY1  13 -1.000000  0.000000  0.000000       77.43000
REMARK 290   SMTRY2  13  0.000000  1.000000  0.000000       77.43000
REMARK 290   SMTRY3  13  0.000000  0.000000 -1.000000       63.00500
REMARK 290   SMTRY1  14  1.000000  0.000000  0.000000       77.43000
REMARK 290   SMTRY2  14  0.000000 -1.000000  0.000000       77.43000
REMARK 290   SMTRY3  14  0.000000  0.000000 -1.000000       63.00500
REMARK 290   SMTRY1  15  0.000000  1.000000  0.000000       77.43000
REMARK 290   SMTRY2  15  1.000000  0.000000  0.000000       77.43000
REMARK 290   SMTRY3  15  0.000000  0.000000 -1.000000       63.00500
REMARK 290   SMTRY1  16  0.000000 -1.000000  0.000000       77.43000
REMARK 290   SMTRY2  16 -1.000000  0.000000  0.000000       77.43000
REMARK 290   SMTRY3  16  0.000000  0.000000 -1.000000       63.00500
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY.  THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 GENERATING THE BIOMOLECULE
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 8540 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 41560 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -1.85 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350   BIOMT1   2  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   2  0.000000 -1.000000  0.000000       -0.00000
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH A2152   LIES ON A SPECIAL POSITION.
REMARK 400
REMARK 400 COMPOUND
REMARK 400 ENGINEERED RESIDUE IN CHAIN A, ASN  45 TO GLN
REMARK 400 ENGINEERED RESIDUE IN CHAIN A, ASN 483 TO GLN
REMARK 400 ENGINEERED RESIDUE IN CHAIN A, ASN 509 TO GLN
REMARK 400 ENGINEERED RESIDUE IN CHAIN A, ASN 514 TO GLN
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     GLU A     1
REMARK 465     ASP A     2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OG   SER A   466  -  F      F A  1546              2.16
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PHE A  43       -1.18     75.19
REMARK 500    LYS A  51      143.54     86.17
REMARK 500    ASP A  54     -175.41     66.83
REMARK 500    ASN A 106       61.28   -158.57
REMARK 500    ALA A 162       72.56   -154.13
REMARK 500    SER A 198     -118.47     57.82
REMARK 500    PRO A 285       -9.37    -57.50
REMARK 500    ASP A 297      -79.54   -133.32
REMARK 500    ASP A 324       53.25   -117.68
REMARK 500    VAL A 361      100.75     54.24
REMARK 500    ASP A 378      -75.54   -148.54
REMARK 500    ASP A 379      -74.13     53.16
REMARK 500    GLN A 380       76.88     54.26
REMARK 500    ARG A 381       72.93     70.04
REMARK 500    PHE A 398      -54.88   -130.00
REMARK 500    GLU A 497      -52.73    101.20
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CHIRAL CENTERS
REMARK 500
REMARK 500 UNEXPECTED CONFIGURATION OF THE FOLLOWING CHIRAL
REMARK 500 CENTER(S) USING IMPROPER CA--C--CB--N CHIRALITY
REMARK 500 FOR AMINO ACIDS AND C1'--N1(N9)--O4'--C2' FOR
REMARK 500 NUCLEIC ACIDS OR EQUIVALENT ANGLE
REMARK 500 M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,6X,F5.1,6X,A1,10X,A1,3X,A16)
REMARK 500
REMARK 500  M RES CSSEQI    IMPROPER   EXPECTED   FOUND DETAILS
REMARK 500    VAL A 361        19.0      L          L   OUTSIDE RANGE
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 610
REMARK 610 MISSING HETEROATOM
REMARK 610 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 610 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 610 I=INSERTION CODE):
REMARK 610   M RES C SSEQI
REMARK 610     EFS A 1544
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NAG A1530
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NAG A1531
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE FUL A1532
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NAG A1533
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE FUC A1534
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NAG A1535
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NAG A1536
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NAG A1537
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NAG A1538
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NAG A1539
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE FUC A1540
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE  CL A1541
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE  NA A1542
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE SO4 A1543
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EFS A1544
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE   F A1546
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 2J4C   RELATED DB: PDB
REMARK 900  STRUCTURE OF HUMAN BUTYRYLCHOLINESTERASE IN
REMARK 900  COMPLEX WITH 10MM HGCL2
REMARK 900 RELATED ID: 1KCJ   RELATED DB: PDB
REMARK 900  MODEL OF (-)-COCAINE-BOUND (-)-COCAINE
REMARK 900  HYDROLASE COMPLEX
REMARK 900 RELATED ID: 2WIK   RELATED DB: PDB
REMARK 900  NONAGED FORM OF HUMAN BUTYRYLCHOLINESTERASE
REMARK 900  INHIBITED BY TABUN ANALOGUE TA6
REMARK 900 RELATED ID: 1P0P   RELATED DB: PDB
REMARK 900  CRYSTAL STRUCTURE OF SOMAN-AGED HUMAN
REMARK 900  BUTYRYLCHOLINESTERASE IN COMPLEX WITH THE
REMARK 900  SUBSTRATE ANALOGBUTYRYLTHIOCHOLINE
REMARK 900 RELATED ID: 1XLU   RELATED DB: PDB
REMARK 900  X-RAY STRUCTURE OF DI-ISOPROPYL-PHOSPHORO-
REMARK 900  FLUORIDATE (DFP)INHIBITED BUTYRYLCHOLINESTERASE
REMARK 900  AFTER AGING
REMARK 900 RELATED ID: 2WIJ   RELATED DB: PDB
REMARK 900  NONAGED FORM OF HUMAN BUTYRYLCHOLINESTERASE
REMARK 900  INHIBITED BY TABUN ANALOGUE TA5
REMARK 900 RELATED ID: 1XLV   RELATED DB: PDB
REMARK 900  ETHYLPHOSPHORYLATED BUTYRYLCHOLINESTERASE (AGED)
REMARK 900  OBTAINEDBY REACTION WITH ECHOTHIOPHATE
REMARK 900 RELATED ID: 1EHO   RELATED DB: PDB
REMARK 900  MODEL OF (-)-COCAINE-BOUND BCHE COMPLEX.
REMARK 900 RELATED ID: 1P0M   RELATED DB: PDB
REMARK 900  CRYSTAL STRUCTURE OF HUMAN BUTYRYL
REMARK 900  CHOLINESTERASE INCOMPLEX WITH A CHOLINE
REMARK 900  MOLECULE
REMARK 900 RELATED ID: 1XLW   RELATED DB: PDB
REMARK 900  DIETHYLPHOSPHORYLATED BUTYRYLCHOLINESTERASE (NONAGED
REMARK 900  )OBTAINED BY REACTION WITH ECHOTHIOPHATE
REMARK 900 RELATED ID: 1EHQ   RELATED DB: PDB
REMARK 900  MODEL OF (+)-COCAINE-BOUND BCHE COMPLEX
REMARK 900 RELATED ID: 1P0Q   RELATED DB: PDB
REMARK 900  CRYSTAL STRUCTURE OF SOMAN-AGED HUMAN
REMARK 900  BUTYRYL CHOLINESTERASE
REMARK 900 RELATED ID: 2WID   RELATED DB: PDB
REMARK 900  AGED FORM OF HUMAN BUTYRYLCHOLINESTERASE
REMARK 900  INHIBITED BY TABUN ANALOGUE TA1
REMARK 900 RELATED ID: 2WIL   RELATED DB: PDB
REMARK 900  AGED FORM OF HUMAN BUTYRYLCHOLINESTERASE
REMARK 900  INHIBITED BY TABUN ANALOGUE TA5
REMARK 900 RELATED ID: 2WIF   RELATED DB: PDB
REMARK 900  AGED FORM OF HUMAN BUTYRYLCHOLINESTERASE
REMARK 900  INHIBITED BY TABUN ANALOGUE TA1
REMARK 900 RELATED ID: 1P0I   RELATED DB: PDB
REMARK 900  CRYSTAL STRUCTURE OF HUMAN BUTYRYL
REMARK 900  CHOLINESTERASE
REMARK 900 RELATED ID: 2WIG   RELATED DB: PDB
REMARK 900  NONAGED FORM OF HUMAN BUTYRYLCHOLINESTERASE
REMARK 900  INHIBITED BY TABUN ANALOGUE TA4
DBREF  2WSL A    1   529  UNP    P06276   CHLE_HUMAN      29    557
SEQADV 2WSL GLN A   17  UNP  P06276    ASN    45 ENGINEERED MUTATION
SEQADV 2WSL GLN A  455  UNP  P06276    ASN   483 ENGINEERED MUTATION
SEQADV 2WSL GLN A  481  UNP  P06276    ASN   509 ENGINEERED MUTATION
SEQADV 2WSL GLN A  486  UNP  P06276    ASN   514 ENGINEERED MUTATION
SEQRES   1 A  529  GLU ASP ASP ILE ILE ILE ALA THR LYS ASN GLY LYS VAL
SEQRES   2 A  529  ARG GLY MET GLN LEU THR VAL PHE GLY GLY THR VAL THR
SEQRES   3 A  529  ALA PHE LEU GLY ILE PRO TYR ALA GLN PRO PRO LEU GLY
SEQRES   4 A  529  ARG LEU ARG PHE LYS LYS PRO GLN SER LEU THR LYS TRP
SEQRES   5 A  529  SER ASP ILE TRP ASN ALA THR LYS TYR ALA ASN SER CYS
SEQRES   6 A  529  CSS GLN ASN ILE ASP GLN SER PHE PRO GLY PHE HIS GLY
SEQRES   7 A  529  SER GLU MET TRP ASN PRO ASN THR ASP LEU SER GLU ASP
SEQRES   8 A  529  CYS LEU TYR LEU ASN VAL TRP ILE PRO ALA PRO LYS PRO
SEQRES   9 A  529  LYS ASN ALA THR VAL LEU ILE TRP ILE TYR GLY GLY GLY
SEQRES  10 A  529  PHE GLN THR GLY THR SER SER LEU HIS VAL TYR ASP GLY
SEQRES  11 A  529  LYS PHE LEU ALA ARG VAL GLU ARG VAL ILE VAL VAL SER
SEQRES  12 A  529  MET ASN TYR ARG VAL GLY ALA LEU GLY PHE LEU ALA LEU
SEQRES  13 A  529  PRO GLY ASN PRO GLU ALA PRO GLY ASN MET GLY LEU PHE
SEQRES  14 A  529  ASP GLN GLN LEU ALA LEU GLN TRP VAL GLN LYS ASN ILE
SEQRES  15 A  529  ALA ALA PHE GLY GLY ASN PRO LYS SER VAL THR LEU PHE
SEQRES  16 A  529  GLY GLU SER ALA GLY ALA ALA SER VAL SER LEU HIS LEU
SEQRES  17 A  529  LEU SER PRO GLY SER HIS SER LEU PHE THR ARG ALA ILE
SEQRES  18 A  529  LEU GLN SER GLY SER PHE ASN ALA PRO TRP ALA VAL THR
SEQRES  19 A  529  SER LEU TYR GLU ALA ARG ASN ARG THR LEU ASN LEU ALA
SEQRES  20 A  529  LYS LEU THR GLY CYS SER ARG GLU ASN GLU THR GLU ILE
SEQRES  21 A  529  ILE LYS CYS LEU ARG ASN LYS ASP PRO GLN GLU ILE LEU
SEQRES  22 A  529  LEU ASN GLU ALA PHE VAL VAL PRO TYR GLY THR PRO LEU
SEQRES  23 A  529  SER VAL ASN PHE GLY PRO THR VAL ASP GLY ASP PHE LEU
SEQRES  24 A  529  THR ASP MET PRO ASP ILE LEU LEU GLU LEU GLY GLN PHE
SEQRES  25 A  529  LYS LYS THR GLN ILE LEU VAL GLY VAL ASN LYS ASP GLU
SEQRES  26 A  529  GLY THR ALA PHE LEU VAL TYR GLY ALA PRO GLY PHE SER
SEQRES  27 A  529  LYS ASP ASN ASN SER ILE ILE THR ARG LYS GLU PHE GLN
SEQRES  28 A  529  GLU GLY LEU LYS ILE PHE PHE PRO GLY VAL SER GLU PHE
SEQRES  29 A  529  GLY LYS GLU SER ILE LEU PHE HIS TYR THR ASP TRP VAL
SEQRES  30 A  529  ASP ASP GLN ARG PRO GLU ASN TYR ARG GLU ALA LEU GLY
SEQRES  31 A  529  ASP VAL VAL GLY ASP TYR ASN PHE ILE CYS PRO ALA LEU
SEQRES  32 A  529  GLU PHE THR LYS LYS PHE SER GLU TRP GLY ASN ASN ALA
SEQRES  33 A  529  PHE PHE TYR TYR PHE GLU HIS ARG SER SER LYS LEU PRO
SEQRES  34 A  529  TRP PRO GLU TRP MET GLY VAL MET HIS GLY TYR GLU ILE
SEQRES  35 A  529  GLU PHE VAL PHE GLY LEU PRO LEU GLU ARG ARG ASP GLN
SEQRES  36 A  529  TYR THR LYS ALA GLU GLU ILE LEU SER ARG SER ILE VAL
SEQRES  37 A  529  LYS ARG TRP ALA ASN PHE ALA LYS TYR GLY ASN PRO GLN
SEQRES  38 A  529  GLU THR GLN ASN GLN SER THR SER TRP PRO VAL PHE LYS
SEQRES  39 A  529  SER THR GLU GLN LYS TYR LEU THR LEU ASN THR GLU SER
SEQRES  40 A  529  THR ARG ILE MET THR LYS LEU ARG ALA GLN GLN CYS ARG
SEQRES  41 A  529  PHE TRP THR SER PHE PHE PRO LYS VAL
MODRES 2WSL CSS A   66  CYS  S-MERCAPTOCYSTEINE
HET    NAG  A1530      14
HET    NAG  A1531      14
HET    FUL  A1532      10
HET    NAG  A1533      14
HET    FUC  A1534      10
HET    NAG  A1535      14
HET    NAG  A1536      14
HET    NAG  A1537      14
HET    NAG  A1538      14
HET    NAG  A1539      14
HET    FUC  A1540      10
HET     CL  A1541       1
HET     NA  A1542       1
HET    SO4  A1543       5
HET    EFS  A1544       6
HET    CSS  A  66       7
HET      F  A1546       1
HETNAM     NAG N-ACETYL-D-GLUCOSAMINE
HETNAM     EFS ETHYL DIHYDROGEN PHOSPHATE
HETNAM     SO4 SULFATE ION
HETNAM      CL CHLORIDE ION
HETNAM     FUL BETA-L-FUCOSE
HETNAM     FUC ALPHA-L-FUCOSE
HETNAM     CSS S-MERCAPTOCYSTEINE
HETNAM       F FLUORIDE ION
HETNAM      NA SODIUM ION
HETSYN     NAG NAG
HETSYN     FUL 6-DEOXY-BETA-L-GALACTOSE
FORMUL   2  EFS    C2 H7 O4 P
FORMUL   3  NAG    8(C8 H15 N O6)
FORMUL   4  SO4    O4 S 2-
FORMUL   5   CL    CL 1-
FORMUL   6    F    F 1-
FORMUL   7  CSS    C3 H7 N O2 S2
FORMUL   8  FUL    C6 H12 O5
FORMUL   9  FUC    2(C6 H12 O5)
FORMUL  10   NA    NA 1+
FORMUL  11  HOH   *397(H2 O)
HELIX    1   1 LEU A   38  ARG A   42  5                                   5
HELIX    2   2 PHE A   76  MET A   81  1                                   6
HELIX    3   3 LEU A  125  ASP A  129  5                                   5
HELIX    4   4 GLY A  130  ARG A  138  1                                   9
HELIX    5   5 VAL A  148  LEU A  154  1                                   7
HELIX    6   6 ASN A  165  ILE A  182  1                                  18
HELIX    7   7 ALA A  183  PHE A  185  5                                   3
HELIX    8   8 SER A  198  SER A  210  1                                  13
HELIX    9   9 PRO A  211  PHE A  217  5                                   7
HELIX   10  10 SER A  235  THR A  250  1                                  16
HELIX   11  11 ASN A  256  ARG A  265  1                                  10
HELIX   12  12 ASP A  268  GLU A  276  1                                   9
HELIX   13  13 ALA A  277  VAL A  280  5                                   4
HELIX   14  14 MET A  302  LEU A  309  1                                   8
HELIX   15  15 GLY A  326  GLY A  333  5                                   8
HELIX   16  16 THR A  346  PHE A  358  1                                  13
HELIX   17  17 SER A  362  THR A  374  1                                  13
HELIX   18  18 GLU A  383  PHE A  398  1                                  16
HELIX   19  19 PHE A  398  GLU A  411  1                                  14
HELIX   20  20 PRO A  431  GLY A  435  5                                   5
HELIX   21  21 GLU A  441  PHE A  446  1                                   6
HELIX   22  22 GLY A  447  GLN A  455  5                                   9
HELIX   23  23 THR A  457  GLY A  478  1                                  22
HELIX   24  24 ARG A  515  PHE A  525  1                                  11
SHEET    1   A 3 ILE A   5  THR A   8  0
SHEET    2   A 3 GLY A  11  ARG A  14 -1  O  VAL A  13   N  ILE A   6
SHEET    3   A 3 ILE A  55  ASN A  57  1  O  TRP A  56   N  ARG A  14
SHEET    1   B11 MET A  16  VAL A  20  0
SHEET    2   B11 GLY A  23  PRO A  32 -1  O  VAL A  25   N  LEU A  18
SHEET    3   B11 TYR A  94  PRO A 100 -1  O  VAL A  97   N  PHE A  28
SHEET    4   B11 ILE A 140  MET A 144 -1  O  SER A 143   N  ASN A  96
SHEET    5   B11 ALA A 107  ILE A 113  1  N  TRP A 112   O  VAL A 142
SHEET    6   B11 GLY A 187  GLU A 197  1  O  THR A 193   N  VAL A 109
SHEET    7   B11 ARG A 219  GLN A 223  1  O  GLN A 223   N  GLY A 196
SHEET    8   B11 ILE A 317  ASN A 322  1  O  LEU A 318   N  LEU A 222
SHEET    9   B11 ALA A 416  PHE A 421  1  O  PHE A 421   N  VAL A 321
SHEET   10   B11 LYS A 499  LEU A 503  1  O  LEU A 503   N  TYR A 420
SHEET   11   B11 ILE A 510  THR A 512 -1  O  MET A 511   N  TYR A 500
SSBOND   1 CYS A   65    CYS A   92                          1555   1555  2.06
SSBOND   2 CYS A  252    CYS A  263                          1555   1555  2.05
SSBOND   3 CYS A  400    CYS A  519                          1555   1555  2.07
LINK         ND2 ASN A  57                 C1  NAG A1535     1555   1555  1.44
LINK         C   CYS A  65                 N   CSS A  66     1555   1555  1.33
LINK         C   CSS A  66                 N   GLN A  67     1555   1555  1.33
LINK         ND2 ASN A 106                 C1  NAG A1533     1555   1555  1.43
LINK         OG  SER A 198                 P   EFS A1544     1555   1555  1.61
LINK         ND2 ASN A 241                 C1  NAG A1538     1555   1555  1.45
LINK         ND2 ASN A 256                 C1  NAG A1537     1555   1555  1.44
LINK         ND2 ASN A 341                 C1  NAG A1530     1555   1555  1.43
LINK         ND2 ASN A 485                 C1  NAG A1536     1555   1555  1.44
LINK         O6  NAG A1530                 C1  FUL A1532     1555   1555  1.45
LINK         O4  NAG A1530                 C1  NAG A1531     1555   1555  1.44
LINK         O6  NAG A1533                 C1  FUC A1534     1555   1555  1.45
LINK         O6  NAG A1538                 C1  FUC A1540     1555   1555  1.44
LINK         O4  NAG A1538                 C1  NAG A1539     1555   1555  1.45
CISPEP   1 ALA A  101    PRO A  102          0         0.37
CISPEP   2 GLY A  360    VAL A  361          0         7.00
CISPEP   3 VAL A  377    ASP A  378          0       -11.92
CISPEP   4 GLN A  380    ARG A  381          0        16.21
SITE     1 AC1  5 SER A 338  ASN A 341  NAG A1531  FUL A1532
SITE     2 AC1  5 HOH A2232
SITE     1 AC2  3 PRO A 335  GLY A 336  NAG A1530
SITE     1 AC3  1 NAG A1530
SITE     1 AC4  5 ASN A 106  ASN A 188  LYS A 190  FUC A1534
SITE     2 AC4  5 HOH A2388
SITE     1 AC5  9 ASN A 188  LYS A 190  SER A 191  NAG A1533
SITE     2 AC5  9 HOH A2323  HOH A2387  HOH A2388  HOH A2389
SITE     3 AC5  9 HOH A2390
SITE     1 AC6  2 ASN A  57  HOH A2391
SITE     1 AC7  5 ARG A 465  LYS A 469  ASN A 485  HOH A2319
SITE     2 AC7  5 HOH A2392
SITE     1 AC8  4 ASN A 256  THR A 258  HOH A2393  HOH A2394
SITE     1 AC9  6 TYR A 237  ASN A 241  ASN A 245  NAG A1539
SITE     2 AC9  6 FUC A1540  HOH A2395
SITE     1 BC1  2 NAG A1538  FUC A1540
SITE     1 BC2  5 ASN A 245  LEU A 249  PHE A 278  NAG A1538
SITE     2 BC2  5 NAG A1539
SITE     1 BC3  2 GLN A 351  HOH A2241
SITE     1 BC4  2 HIS A 372  PHE A 525
SITE     1 BC5  5 GLN A 316  GLY A 413  ASN A 414  ASN A 415
SITE     2 BC5  5 HOH A2396
SITE     1 BC6  6 GLY A 116  GLY A 117  SER A 198  ALA A 199
SITE     2 BC6  6 TRP A 231  HIS A 438
SITE     1 BC7  4 SER A 466  ARG A 470  HOH A2336  HOH A2364
CRYST1  154.860  154.860  126.010  90.00  90.00  90.00 I 4 2 2      16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.006457  0.000000  0.000000        0.00000
SCALE2      0.000000  0.006457  0.000000        0.00000
SCALE3      0.000000  0.000000  0.007936        0.00000
TER    4265      VAL A 529
MASTER      522    0   17   24   14    0   25    6 4817    1  175   41
END