longtext: 2WTM-pdb

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HEADER    HYDROLASE                               17-SEP-09   2WTM
TITLE     EST1E FROM BUTYRIVIBRIO PROTEOCLASTICUS
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: EST1E;
COMPND   3 CHAIN: A, B, C, D;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: CLOSTRIDIUM PROTEOCLASTICUM;
SOURCE   3 ORGANISM_TAXID: 43305;
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   5 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE   6 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)
KEYWDS    ESTERASE, HYDROLASE, FERULIC ACID ESTERASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    D.C.GOLDSTONE,V.L.ARCUS
REVDAT   1   19-JAN-10 2WTM    0
JRNL        AUTH   D.C.GOLDSTONE,S.G.VILLAS-BOAS,M.TILL,W.J.KELLY,
JRNL        AUTH 2 G.T.ATTWOOD,V.L.ARCUS
JRNL        TITL   STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF A
JRNL        TITL 2 PROMISCUOUS FERULOYL ESTERASE (EST1E) FROM THE
JRNL        TITL 3 RUMEN BACTERIUM BUTYRIVIBRIO PROTEOCLASTICUS.
JRNL        REF    PROTEINS                                   2009
JRNL        REFN                   ESSN 1097-0134
JRNL        PMID   20058325
JRNL        DOI    10.1002/PROT.22662
REMARK   2
REMARK   2 RESOLUTION.    1.60 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.2.0005
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.60
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 89.44
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NONE
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.42
REMARK   3   NUMBER OF REFLECTIONS             : 124101
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.15793
REMARK   3   R VALUE            (WORKING SET) : 0.15604
REMARK   3   FREE R VALUE                     : 0.19410
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.0
REMARK   3   FREE R VALUE TEST SET COUNT      : 6580
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.600
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.642
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 9181
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 99.63
REMARK   3   BIN R VALUE           (WORKING SET) : 0.206
REMARK   3   BIN FREE R VALUE SET COUNT          : 508
REMARK   3   BIN FREE R VALUE                    : 0.257
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 7757
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 66
REMARK   3   SOLVENT ATOMS            : 886
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 16.4
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 15.150
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.57
REMARK   3    B22 (A**2) : -0.32
REMARK   3    B33 (A**2) : -0.60
REMARK   3    B12 (A**2) : 0.00
REMARK   3    B13 (A**2) : -0.82
REMARK   3    B23 (A**2) : 0.00
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.079
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.084
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.054
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 1.506
REMARK   3
REMARK   3  CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.971
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.954
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  7992 ; 0.022 ; 0.022
REMARK   3   BOND LENGTHS OTHERS               (A):  7263 ; 0.001 ; 0.020
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 10866 ; 1.905 ; 1.970
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 16941 ; 0.957 ; 3.000
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  1011 ; 6.157 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   355 ;33.258 ;25.099
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1354 ;12.210 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    27 ;16.571 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1227 ; 0.127 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  8854 ; 0.010 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):  1501 ; 0.002 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  1670 ; 0.236 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  7624 ; 0.185 ; 0.200
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  3973 ; 0.184 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  4619 ; 0.088 ; 0.200
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   689 ; 0.199 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):    32 ; 0.195 ; 0.200
REMARK   3   SYMMETRY VDW OTHERS               (A):    91 ; 0.225 ; 0.200
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):    36 ; 0.264 ; 0.200
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  6341 ; 1.647 ; 1.500
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  2017 ; 0.390 ; 1.500
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  8004 ; 1.920 ; 2.000
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  3496 ; 3.333 ; 3.000
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  2847 ; 4.569 ; 4.500
REMARK   3
REMARK   3  ANISOTROPIC THERMAL FACTOR RESTRAINTS.   COUNT   RMS    WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : BABINET MODEL WITH MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.40
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE
REMARK   3   RIDING POSITIONS.
REMARK   4
REMARK   4 2WTM COMPLIES WITH FORMAT V. 3.20, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 17-SEP-09.
REMARK 100 THE PDBE ID CODE IS EBI-41175.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : NULL
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRL
REMARK 200  BEAMLINE                       : BL9-2
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.979
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 124101
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.60
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.00
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.0
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.4
REMARK 200  DATA REDUNDANCY                : 2.8
REMARK 200  R MERGE                    (I) : 0.08
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 13.70
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.60
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.65
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.6
REMARK 200  DATA REDUNDANCY IN SHELL       : 2.8
REMARK 200  R MERGE FOR SHELL          (I) : 0.35
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 4.80
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NONE
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS  (%): 40.54
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.08
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: NULL
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       54.53350
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY.  THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 GENERATING THE BIOMOLECULE
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 3910 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 18450 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -29.97 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 3930 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 18280 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -28.62 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     SER A    -2
REMARK 465     HIS B    64
REMARK 465     GLY B    65
REMARK 465     LYS B    66
REMARK 465     SER B    67
REMARK 465     ASP B    68
REMARK 465     GLY B    69
REMARK 465     LYS B    70
REMARK 465     PHE B    71
REMARK 465     GLU B    72
REMARK 465     LYS C   248
REMARK 465     GLY D    65
REMARK 465     LYS D    66
REMARK 465     SER D    67
REMARK 465     ASP D    68
REMARK 465     GLY D    69
REMARK 465     LYS D    70
REMARK 465     PHE D    71
REMARK 465     GLU D    72
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     LYS A  18    CG   CD   CE   NZ
REMARK 470     ASN A  20    CG   OD1  ND2
REMARK 470     GLU A  22    CD   OE1  OE2
REMARK 470     LYS A  70    NZ
REMARK 470     LYS A 248    CG   CD   CE   NZ
REMARK 470     LYS B  18    CG   CD   CE   NZ
REMARK 470     ARG B 163    CG   CD   NE   CZ   NH1  NH2
REMARK 470     LYS B 164    CG   CD   CE   NZ
REMARK 470     LYS B 213    CE   NZ
REMARK 470     LYS C   9    NZ
REMARK 470     LYS C  18    CG   CD   CE   NZ
REMARK 470     GLU C  22    CG   CD   OE1  OE2
REMARK 470     LYS C 216    NZ
REMARK 470     LYS D   9    CD   CE   NZ
REMARK 470     LYS D  18    CG   CD   CE   NZ
REMARK 470     ASN D  19    CG   OD1  ND2
REMARK 470     ASN D  20    CG   OD1  ND2
REMARK 470     GLU D  22    CG   CD   OE1  OE2
REMARK 470     HIS D  64    CG   ND1  CD2  CE1  NE2
REMARK 470     ASP D  94    CG   OD1  OD2
REMARK 470     LYS D 164    CE   NZ
REMARK 470     LYS D 184    CE   NZ
REMARK 470     GLU D 198    CD   OE1  OE2
REMARK 470     LYS D 248    CG   CD   CE   NZ
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    ARG A   119     O    HOH A  2111              1.97
REMARK 500   OE1  GLU A   203     O    HOH A  2183              2.13
REMARK 500   OE1  GLU B    47     O    HOH B  2044              2.20
REMARK 500   OE1  GLU C    47     O    HOH C  2059              2.05
REMARK 500   N    ASP D    73     O    HOH D  2054              1.78
REMARK 500   OE1  GLU D   151     O    HOH D  2117              2.06
REMARK 500   O1   GOL A  1250     O    HOH A  2215              2.15
REMARK 500   O    HOH A  2007     O    HOH A  2016              2.17
REMARK 500   O    HOH B  2009     O    HOH A  2038              2.06
REMARK 500   O    HOH B  2063     O    HOH A  2096              2.20
REMARK 500   O    HOH B  2169     O    HOH B  2170              2.12
REMARK 500   O    HOH B  2212     O    HOH B  2213              2.13
REMARK 500   O    HOH C  2043     O    HOH C  2122              1.97
REMARK 500   O    HOH C  2081     O    HOH C  2087              2.19
REMARK 500   O    HOH C  2092     O    HOH C  2095              2.16
REMARK 500   O    HOH C  2163     O    HOH C  2166              2.07
REMARK 500   O    HOH D  2023     O    HOH D  2189              2.19
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   O    HOH B  2091     O    HOH C  2212     1457     2.13
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    CYS B 215   CB    CYS B 215   SG     -0.099
REMARK 500    GLU C 198   CB    GLU C 198   CG      0.118
REMARK 500    GLU D 203   CG    GLU D 203   CD      0.092
REMARK 500    CYS D 215   CB    CYS D 215   SG     -0.102
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    PRO A  21   C   -  N   -  CD  ANGL. DEV. = -14.1 DEGREES
REMARK 500    ASP A 195   CB  -  CG  -  OD2 ANGL. DEV. =  -5.4 DEGREES
REMARK 500    ARG B  40   NE  -  CZ  -  NH1 ANGL. DEV. =   3.7 DEGREES
REMARK 500    ARG B  40   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.2 DEGREES
REMARK 500    ASP B 180   CB  -  CG  -  OD2 ANGL. DEV. =  -7.4 DEGREES
REMARK 500    PHE C  77   CB  -  CG  -  CD1 ANGL. DEV. =   4.7 DEGREES
REMARK 500    PHE C  77   CB  -  CG  -  CD2 ANGL. DEV. =  -5.5 DEGREES
REMARK 500    PRO C 127   C   -  N   -  CD  ANGL. DEV. = -13.9 DEGREES
REMARK 500    PRO D 127   C   -  N   -  CD  ANGL. DEV. = -13.3 DEGREES
REMARK 500    ARG D 139   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.8 DEGREES
REMARK 500    ASP D 155   CB  -  CG  -  OD1 ANGL. DEV. =   6.1 DEGREES
REMARK 500    ARG D 177   NE  -  CZ  -  NH1 ANGL. DEV. =   3.7 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASP A   6     -123.84     46.59
REMARK 500    THR A  34       -5.59     75.82
REMARK 500    THR A  97      -92.25    -91.41
REMARK 500    SER A 105     -117.67     59.36
REMARK 500    ALA A 159     -169.38   -166.98
REMARK 500    TRP A 160       62.64     60.68
REMARK 500    HIS A 230       50.41   -140.99
REMARK 500    THR B  97      -86.01    -95.14
REMARK 500    SER B 105     -117.04     62.25
REMARK 500    LEU B 143      114.72   -161.33
REMARK 500    LEU B 144     -119.44     47.52
REMARK 500    TRP B 160      129.54    -36.41
REMARK 500    THR C  34       -6.77     73.17
REMARK 500    THR C  97      -89.99    -95.77
REMARK 500    SER C 105     -117.11     62.40
REMARK 500    ALA C 159     -168.39   -168.33
REMARK 500    HIS C 230       50.63   -147.30
REMARK 500    ASN D  19      -11.68    103.62
REMARK 500    THR D  97      -90.81    -97.85
REMARK 500    SER D 105     -117.08     60.54
REMARK 500    HIS D 230       50.43   -140.80
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CHIRAL CENTERS
REMARK 500
REMARK 500 UNEXPECTED CONFIGURATION OF THE FOLLOWING CHIRAL
REMARK 500 CENTER(S) USING IMPROPER CA--C--CB--N CHIRALITY
REMARK 500 FOR AMINO ACIDS AND C1'--O4'--N1(N9)--C2' FOR
REMARK 500 NUCLEIC ACIDS OR EQUIVALENT ANGLE
REMARK 500 M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,6X,F5.1,6X,A1,10X,A1,3X,A16)
REMARK 500
REMARK 500  M RES CSSEQI    IMPROPER   EXPECTED   FOUND DETAILS
REMARK 500    VAL A 111        24.5      L          L   OUTSIDE RANGE
REMARK 500    SER C  -2        23.1      L          L   OUTSIDE RANGE
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PO4 D1249
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PO4 B1249
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PO4 D1250
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PO4 C1248
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PO4 B1250
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PO4 A1249
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A1250
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL C1249
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL D1251
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B1251
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL D1252
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B1252
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 2WTN   RELATED DB: PDB
REMARK 900  FERULIC ACID BOUND TO EST1E FROM
REMARK 900  BUTYRIVIBRIO PROTEOCLASTICUS
DBREF  2WTM A   -2   248  PDB    2WTM     2WTM            -2    248
DBREF  2WTM B   -2   248  PDB    2WTM     2WTM            -2    248
DBREF  2WTM C   -2   248  PDB    2WTM     2WTM            -2    248
DBREF  2WTM D   -2   248  PDB    2WTM     2WTM            -2    248
SEQRES   1 A  251  SER GLY ALA MET TYR ILE ASP CYS ASP GLY ILE LYS LEU
SEQRES   2 A  251  ASN ALA TYR LEU ASP MET PRO LYS ASN ASN PRO GLU LYS
SEQRES   3 A  251  CYS PRO LEU CYS ILE ILE ILE HIS GLY PHE THR GLY HIS
SEQRES   4 A  251  SER GLU GLU ARG HIS ILE VAL ALA VAL GLN GLU THR LEU
SEQRES   5 A  251  ASN GLU ILE GLY VAL ALA THR LEU ARG ALA ASP MET TYR
SEQRES   6 A  251  GLY HIS GLY LYS SER ASP GLY LYS PHE GLU ASP HIS THR
SEQRES   7 A  251  LEU PHE LYS TRP LEU THR ASN ILE LEU ALA VAL VAL ASP
SEQRES   8 A  251  TYR ALA LYS LYS LEU ASP PHE VAL THR ASP ILE TYR MET
SEQRES   9 A  251  ALA GLY HIS SER GLN GLY GLY LEU SER VAL MET LEU ALA
SEQRES  10 A  251  ALA ALA MET GLU ARG ASP ILE ILE LYS ALA LEU ILE PRO
SEQRES  11 A  251  LEU SER PRO ALA ALA MET ILE PRO GLU ILE ALA ARG THR
SEQRES  12 A  251  GLY GLU LEU LEU GLY LEU LYS PHE ASP PRO GLU ASN ILE
SEQRES  13 A  251  PRO ASP GLU LEU ASP ALA TRP ASP GLY ARG LYS LEU LYS
SEQRES  14 A  251  GLY ASN TYR VAL ARG VAL ALA GLN THR ILE ARG VAL GLU
SEQRES  15 A  251  ASP PHE VAL ASP LYS TYR THR LYS PRO VAL LEU ILE VAL
SEQRES  16 A  251  HIS GLY ASP GLN ASP GLU ALA VAL PRO TYR GLU ALA SER
SEQRES  17 A  251  VAL ALA PHE SER LYS GLN TYR LYS ASN CYS LYS LEU VAL
SEQRES  18 A  251  THR ILE PRO GLY ASP THR HIS CYS TYR ASP HIS HIS LEU
SEQRES  19 A  251  GLU LEU VAL THR GLU ALA VAL LYS GLU PHE MET LEU GLU
SEQRES  20 A  251  GLN ILE ALA LYS
SEQRES   1 B  251  SER GLY ALA MET TYR ILE ASP CYS ASP GLY ILE LYS LEU
SEQRES   2 B  251  ASN ALA TYR LEU ASP MET PRO LYS ASN ASN PRO GLU LYS
SEQRES   3 B  251  CYS PRO LEU CYS ILE ILE ILE HIS GLY PHE THR GLY HIS
SEQRES   4 B  251  SER GLU GLU ARG HIS ILE VAL ALA VAL GLN GLU THR LEU
SEQRES   5 B  251  ASN GLU ILE GLY VAL ALA THR LEU ARG ALA ASP MET TYR
SEQRES   6 B  251  GLY HIS GLY LYS SER ASP GLY LYS PHE GLU ASP HIS THR
SEQRES   7 B  251  LEU PHE LYS TRP LEU THR ASN ILE LEU ALA VAL VAL ASP
SEQRES   8 B  251  TYR ALA LYS LYS LEU ASP PHE VAL THR ASP ILE TYR MET
SEQRES   9 B  251  ALA GLY HIS SER GLN GLY GLY LEU SER VAL MET LEU ALA
SEQRES  10 B  251  ALA ALA MET GLU ARG ASP ILE ILE LYS ALA LEU ILE PRO
SEQRES  11 B  251  LEU SER PRO ALA ALA MET ILE PRO GLU ILE ALA ARG THR
SEQRES  12 B  251  GLY GLU LEU LEU GLY LEU LYS PHE ASP PRO GLU ASN ILE
SEQRES  13 B  251  PRO ASP GLU LEU ASP ALA TRP ASP GLY ARG LYS LEU LYS
SEQRES  14 B  251  GLY ASN TYR VAL ARG VAL ALA GLN THR ILE ARG VAL GLU
SEQRES  15 B  251  ASP PHE VAL ASP LYS TYR THR LYS PRO VAL LEU ILE VAL
SEQRES  16 B  251  HIS GLY ASP GLN ASP GLU ALA VAL PRO TYR GLU ALA SER
SEQRES  17 B  251  VAL ALA PHE SER LYS GLN TYR LYS ASN CYS LYS LEU VAL
SEQRES  18 B  251  THR ILE PRO GLY ASP THR HIS CYS TYR ASP HIS HIS LEU
SEQRES  19 B  251  GLU LEU VAL THR GLU ALA VAL LYS GLU PHE MET LEU GLU
SEQRES  20 B  251  GLN ILE ALA LYS
SEQRES   1 C  251  SER GLY ALA MET TYR ILE ASP CYS ASP GLY ILE LYS LEU
SEQRES   2 C  251  ASN ALA TYR LEU ASP MET PRO LYS ASN ASN PRO GLU LYS
SEQRES   3 C  251  CYS PRO LEU CYS ILE ILE ILE HIS GLY PHE THR GLY HIS
SEQRES   4 C  251  SER GLU GLU ARG HIS ILE VAL ALA VAL GLN GLU THR LEU
SEQRES   5 C  251  ASN GLU ILE GLY VAL ALA THR LEU ARG ALA ASP MET TYR
SEQRES   6 C  251  GLY HIS GLY LYS SER ASP GLY LYS PHE GLU ASP HIS THR
SEQRES   7 C  251  LEU PHE LYS TRP LEU THR ASN ILE LEU ALA VAL VAL ASP
SEQRES   8 C  251  TYR ALA LYS LYS LEU ASP PHE VAL THR ASP ILE TYR MET
SEQRES   9 C  251  ALA GLY HIS SER GLN GLY GLY LEU SER VAL MET LEU ALA
SEQRES  10 C  251  ALA ALA MET GLU ARG ASP ILE ILE LYS ALA LEU ILE PRO
SEQRES  11 C  251  LEU SER PRO ALA ALA MET ILE PRO GLU ILE ALA ARG THR
SEQRES  12 C  251  GLY GLU LEU LEU GLY LEU LYS PHE ASP PRO GLU ASN ILE
SEQRES  13 C  251  PRO ASP GLU LEU ASP ALA TRP ASP GLY ARG LYS LEU LYS
SEQRES  14 C  251  GLY ASN TYR VAL ARG VAL ALA GLN THR ILE ARG VAL GLU
SEQRES  15 C  251  ASP PHE VAL ASP LYS TYR THR LYS PRO VAL LEU ILE VAL
SEQRES  16 C  251  HIS GLY ASP GLN ASP GLU ALA VAL PRO TYR GLU ALA SER
SEQRES  17 C  251  VAL ALA PHE SER LYS GLN TYR LYS ASN CYS LYS LEU VAL
SEQRES  18 C  251  THR ILE PRO GLY ASP THR HIS CYS TYR ASP HIS HIS LEU
SEQRES  19 C  251  GLU LEU VAL THR GLU ALA VAL LYS GLU PHE MET LEU GLU
SEQRES  20 C  251  GLN ILE ALA LYS
SEQRES   1 D  251  SER GLY ALA MET TYR ILE ASP CYS ASP GLY ILE LYS LEU
SEQRES   2 D  251  ASN ALA TYR LEU ASP MET PRO LYS ASN ASN PRO GLU LYS
SEQRES   3 D  251  CYS PRO LEU CYS ILE ILE ILE HIS GLY PHE THR GLY HIS
SEQRES   4 D  251  SER GLU GLU ARG HIS ILE VAL ALA VAL GLN GLU THR LEU
SEQRES   5 D  251  ASN GLU ILE GLY VAL ALA THR LEU ARG ALA ASP MET TYR
SEQRES   6 D  251  GLY HIS GLY LYS SER ASP GLY LYS PHE GLU ASP HIS THR
SEQRES   7 D  251  LEU PHE LYS TRP LEU THR ASN ILE LEU ALA VAL VAL ASP
SEQRES   8 D  251  TYR ALA LYS LYS LEU ASP PHE VAL THR ASP ILE TYR MET
SEQRES   9 D  251  ALA GLY HIS SER GLN GLY GLY LEU SER VAL MET LEU ALA
SEQRES  10 D  251  ALA ALA MET GLU ARG ASP ILE ILE LYS ALA LEU ILE PRO
SEQRES  11 D  251  LEU SER PRO ALA ALA MET ILE PRO GLU ILE ALA ARG THR
SEQRES  12 D  251  GLY GLU LEU LEU GLY LEU LYS PHE ASP PRO GLU ASN ILE
SEQRES  13 D  251  PRO ASP GLU LEU ASP ALA TRP ASP GLY ARG LYS LEU LYS
SEQRES  14 D  251  GLY ASN TYR VAL ARG VAL ALA GLN THR ILE ARG VAL GLU
SEQRES  15 D  251  ASP PHE VAL ASP LYS TYR THR LYS PRO VAL LEU ILE VAL
SEQRES  16 D  251  HIS GLY ASP GLN ASP GLU ALA VAL PRO TYR GLU ALA SER
SEQRES  17 D  251  VAL ALA PHE SER LYS GLN TYR LYS ASN CYS LYS LEU VAL
SEQRES  18 D  251  THR ILE PRO GLY ASP THR HIS CYS TYR ASP HIS HIS LEU
SEQRES  19 D  251  GLU LEU VAL THR GLU ALA VAL LYS GLU PHE MET LEU GLU
SEQRES  20 D  251  GLN ILE ALA LYS
HET    PO4  D1249       5
HET    PO4  B1249       5
HET    PO4  D1250       5
HET    PO4  C1248       5
HET    PO4  B1250       5
HET    PO4  A1249       5
HET    GOL  A1250       6
HET    GOL  C1249       6
HET    GOL  D1251       6
HET    GOL  B1251       6
HET    GOL  D1252       6
HET    GOL  B1252       6
HETNAM     PO4 PHOSPHATE ION
HETNAM     GOL GLYCEROL
FORMUL   5  PO4    6(O4 P 3-)
FORMUL   6  GOL    6(C3 H8 O3)
FORMUL   7  HOH   *886(H2 O)
HELIX    1   1 GLU A   39  ILE A   52  1                                  14
HELIX    2   2 LYS A   70  HIS A   74  5                                   5
HELIX    3   3 THR A   75  LYS A   91  1                                  17
HELIX    4   4 LEU A  109  GLU A  118  1                                  10
HELIX    5   5 MET A  133  THR A  140  1                                   8
HELIX    6   6 ASN A  168  GLN A  174  1                                   7
HELIX    7   7 ARG A  177  TYR A  185  1                                   9
HELIX    8   8 PRO A  201  TYR A  212  1                                  12
HELIX    9   9 HIS A  230  ALA A  247  1                                  18
HELIX   10  10 GLU B   39  ILE B   52  1                                  14
HELIX   11  11 THR B   75  LYS B   92  1                                  18
HELIX   12  12 LEU B  109  GLU B  118  1                                  10
HELIX   13  13 MET B  133  THR B  140  1                                   8
HELIX   14  14 ASN B  168  GLN B  174  1                                   7
HELIX   15  15 ARG B  177  TYR B  185  1                                   9
HELIX   16  16 PRO B  201  TYR B  212  1                                  12
HELIX   17  17 HIS B  230  ALA B  247  1                                  18
HELIX   18  18 GLU C   39  ILE C   52  1                                  14
HELIX   19  19 LYS C   70  HIS C   74  5                                   5
HELIX   20  20 THR C   75  LYS C   91  1                                  17
HELIX   21  21 LEU C  109  GLU C  118  1                                  10
HELIX   22  22 MET C  133  THR C  140  1                                   8
HELIX   23  23 ASN C  168  GLN C  174  1                                   7
HELIX   24  24 ARG C  177  TYR C  185  1                                   9
HELIX   25  25 PRO C  201  TYR C  212  1                                  12
HELIX   26  26 HIS C  230  ALA C  247  1                                  18
HELIX   27  27 GLU D   39  ILE D   52  1                                  14
HELIX   28  28 THR D   75  LYS D   91  1                                  17
HELIX   29  29 LEU D  109  GLU D  118  1                                  10
HELIX   30  30 MET D  133  THR D  140  1                                   8
HELIX   31  31 ASN D  168  GLN D  174  1                                   7
HELIX   32  32 ARG D  177  TYR D  185  1                                   9
HELIX   33  33 PRO D  201  TYR D  212  1                                  12
HELIX   34  34 HIS D  230  LYS D  248  1                                  19
SHEET    1  AA 6 MET A   1  ASP A   4  0
SHEET    2  AA 6 LYS A   9  ASP A  15 -1  O  LEU A  10   N  ILE A   3
SHEET    3  AA 6 ALA A  55  ALA A  59 -1  O  THR A  56   N  ASP A  15
SHEET    4  AA 6 CYS A  24  ILE A  30  1  O  PRO A  25   N  ALA A  55
SHEET    5  AA 6 VAL A  96  ALA A 102  1  N  THR A  97   O  CYS A  24
SHEET    6  AA 6 ILE A 122  LEU A 125  1  N  LYS A 123   O  ILE A  99
SHEET    1  AB 2 LEU A 157  ASP A 158  0
SHEET    2  AB 2 LYS A 164  LEU A 165 -1  O  LEU A 165   N  LEU A 157
SHEET    1  AC 2 VAL A 189  HIS A 193  0
SHEET    2  AC 2 CYS A 215  THR A 219  1  O  LYS A 216   N  ILE A 191
SHEET    1  BA 6 MET B   1  ASP B   4  0
SHEET    2  BA 6 LYS B   9  ASP B  15 -1  O  LEU B  10   N  ILE B   3
SHEET    3  BA 6 ALA B  55  ALA B  59 -1  O  THR B  56   N  ASP B  15
SHEET    4  BA 6 CYS B  24  ILE B  30  1  O  PRO B  25   N  ALA B  55
SHEET    5  BA 6 VAL B  96  ALA B 102  1  N  THR B  97   O  CYS B  24
SHEET    6  BA 6 ILE B 122  LEU B 125  1  N  LYS B 123   O  ILE B  99
SHEET    1  BB 3 LYS B 147  PHE B 148  0
SHEET    2  BB 3 LEU C 157  ASP C 158  1  N  ASP C 158   O  LYS B 147
SHEET    3  BB 3 LYS C 164  LEU C 165 -1  O  LEU C 165   N  LEU C 157
SHEET    1  BC 2 LEU B 157  ASP B 158  0
SHEET    2  BC 2 LYS B 164  LEU B 165 -1  O  LEU B 165   N  LEU B 157
SHEET    1  BD 2 VAL B 189  HIS B 193  0
SHEET    2  BD 2 CYS B 215  THR B 219  1  O  LYS B 216   N  ILE B 191
SHEET    1  CA 6 MET C   1  ASP C   4  0
SHEET    2  CA 6 LYS C   9  ASP C  15 -1  O  LEU C  10   N  ILE C   3
SHEET    3  CA 6 ALA C  55  ALA C  59 -1  O  THR C  56   N  ASP C  15
SHEET    4  CA 6 CYS C  24  ILE C  30  1  O  PRO C  25   N  ALA C  55
SHEET    5  CA 6 VAL C  96  ALA C 102  1  N  THR C  97   O  CYS C  24
SHEET    6  CA 6 ILE C 122  LEU C 125  1  N  LYS C 123   O  ILE C  99
SHEET    1  CB 2 VAL C 189  HIS C 193  0
SHEET    2  CB 2 CYS C 215  THR C 219  1  O  LYS C 216   N  ILE C 191
SHEET    1  DA 6 MET D   1  ASP D   4  0
SHEET    2  DA 6 LYS D   9  ASP D  15 -1  O  LEU D  10   N  ILE D   3
SHEET    3  DA 6 ALA D  55  ALA D  59 -1  O  THR D  56   N  ASP D  15
SHEET    4  DA 6 CYS D  24  ILE D  30  1  O  PRO D  25   N  ALA D  55
SHEET    5  DA 6 VAL D  96  ALA D 102  1  N  THR D  97   O  CYS D  24
SHEET    6  DA 6 ILE D 122  LEU D 125  1  N  LYS D 123   O  ILE D  99
SHEET    1  DB 2 LEU D 157  ASP D 158  0
SHEET    2  DB 2 LYS D 164  LEU D 165 -1  O  LEU D 165   N  LEU D 157
SHEET    1  DC 2 VAL D 189  HIS D 193  0
SHEET    2  DC 2 CYS D 215  THR D 219  1  O  LYS D 216   N  ILE D 191
SITE     1 AC1 10 GLY D  32  PHE D  33  THR D  34  SER D 105
SITE     2 AC1 10 GLN D 106  ARG D 163  PO4 D1250  HOH D2191
SITE     3 AC1 10 HOH D2192  HOH D2193
SITE     1 AC2  9 GLY B  32  PHE B  33  THR B  34  SER B 105
SITE     2 AC2  9 GLN B 106  PO4 B1250  HOH B2208  HOH B2209
SITE     3 AC2  9 HOH B2210
SITE     1 AC3 12 HIS D  31  GLY D  32  PHE D  33  THR D  34
SITE     2 AC3 12 GLY D  35  GLU D  39  HIS D  41  HIS D 104
SITE     3 AC3 12 SER D 105  PO4 D1249  HOH D2193  HOH D2194
SITE     1 AC4  7 GLY C  32  PHE C  33  SER C 105  GLN C 106
SITE     2 AC4  7 HIS C 225  GOL C1249  HOH C2255
SITE     1 AC5 12 HIS B  31  GLY B  32  PHE B  33  THR B  34
SITE     2 AC5 12 GLY B  35  GLU B  39  HIS B  41  HIS B 104
SITE     3 AC5 12 SER B 105  PO4 B1249  HOH B2208  HOH B2211
SITE     1 AC6  6 GLY A  32  PHE A  33  SER A 105  GLN A 106
SITE     2 AC6  6 HIS A 225  HOH A2213
SITE     1 AC7  6 HOH A2214  HOH A2215  HOH A2216  GLU B 203
SITE     2 AC7  6 ALA B 207  HOH B2165
SITE     1 AC8 10 PHE C  33  THR C  34  GLY C  35  GLU C  39
SITE     2 AC8 10 HIS C 225  CYS C 226  PO4 C1248  HOH C2257
SITE     3 AC8 10 HOH C2258  HOH C2259
SITE     1 AC9  5 TYR D  13  GLU D  38  ARG D  58  HOH D2034
SITE     2 AC9  5 HOH D2195
SITE     1 BC1  6 TYR B  13  GLU B  38  ARG B  58  HOH B2043
SITE     2 BC1  6 HOH B2212  HOH B2213
SITE     1 BC2  7 HOH C2096  HOH C2104  CYS D   5  ASP D   6
SITE     2 BC2  7 HOH D2071  HOH D2196  HOH D2197
SITE     1 BC3  6 ARG A 119  LYS A 184  ARG B 139  GLU B 151
SITE     2 BC3  6 GLN B 174  HOH B2214
CRYST1   52.035  109.067   91.647  90.00 102.18  90.00 P 1 21 1      8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.019218  0.000000  0.004148        0.00000
SCALE2      0.000000  0.009169  0.000000        0.00000
SCALE3      0.000000  0.000000  0.011163        0.00000
TER    1973      LYS A 248
TER    3879      LYS B 248
TER    5859      ALA C 247
TER    7761      LYS D 248
MASTER      499    0   12   34   41    0   29    6 8709    4   66   80
END