longtext: 3DCN-pdb

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HEADER    HYDROLASE                               04-JUN-08   3DCN
TITLE     GLOMERELLA CINGULATA APO CUTINASE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: CUTINASE;
COMPND   3 CHAIN: A;
COMPND   4 FRAGMENT: UNP RESIDUES 31-224;
COMPND   5 SYNONYM: CUTIN HYDROLASE;
COMPND   6 EC: 3.1.1.74;
COMPND   7 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: GLOMERELLA CINGULATA;
SOURCE   3 ORGANISM_COMMON: ANTHRACNOSE FUNGUS;
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   5 EXPRESSION_SYSTEM_STRAIN: ORIGAMI (DE3);
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: PET32B_CUTA
KEYWDS    GLOMERELLA CINGULATA, CUTINASE, CATALYTIC TRIAD, HYDROLASE,
KEYWDS   2 SECRETED, SERINE ESTERASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    M.P.NYON,D.W.RICE,J.M.BERRISFORD,A.M.HOUNSLOW,A.J.G.MOIR,
AUTHOR   2 H.HUANG,S.NATHAN,N.M.MAHADI,A.B.FARAH DIBA,C.J.CRAVEN
REVDAT   1   18-NOV-08 3DCN    0
JRNL        AUTH   M.P.NYON,D.W.RICE,J.M.BERRISFORD,A.M.HOUNSLOW,
JRNL        AUTH 2 A.J.G.MOIR,H.HUANG,S.NATHAN,N.M.MAHADI,F.D.A.BAKAR,
JRNL        AUTH 3 C.J.CRAVEN
JRNL        TITL   CATALYSIS BY GLOMERELLA CINGULATA CUTINASE
JRNL        TITL 2 REQUIRES CONFORMATIONAL CYCLING BETWEEN THE ACTIVE
JRNL        TITL 3 AND INACTIVE STATES OF ITS CATALYTIC TRIAD
JRNL        REF    J.MOL.BIOL.                                2008
JRNL        REFN   ASTM JMOBAK  UK ESSN 1089-8638
REMARK   1
REMARK   2
REMARK   2 RESOLUTION. 1.90 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.2.0019
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.90
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.25
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.0
REMARK   3   NUMBER OF REFLECTIONS             : 11712
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.196
REMARK   3   R VALUE            (WORKING SET) : 0.190
REMARK   3   FREE R VALUE                     : 0.253
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 9.900
REMARK   3   FREE R VALUE TEST SET COUNT      : 1281
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH           : 1.90
REMARK   3   BIN RESOLUTION RANGE LOW            : 1.95
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 862
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 100.00
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2900
REMARK   3   BIN FREE R VALUE SET COUNT          : 80
REMARK   3   BIN FREE R VALUE                    : 0.4290
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   ALL ATOMS                : 1595
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 22.44
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 21.32
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.01000
REMARK   3    B22 (A**2) : 0.01000
REMARK   3    B33 (A**2) : -0.01000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.204
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.184
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.137
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 4.756
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.952
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.915
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  1476 ; 0.017 ; 0.022
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  2012 ; 1.513 ; 1.959
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   194 ; 6.031 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):    59 ;37.709 ;24.237
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   223 ;13.754 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):     8 ;13.855 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   232 ; 0.102 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  1128 ; 0.007 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):   804 ; 0.196 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  1024 ; 0.307 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   120 ; 0.165 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):    51 ; 0.202 ; 0.200
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):    16 ; 0.170 ; 0.200
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):   989 ; 1.192 ; 1.500
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  1550 ; 1.853 ; 2.000
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):   549 ; 2.834 ; 3.000
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):   462 ; 3.965 ; 4.500
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 3DCN COMPLIES WITH FORMAT V. 3.1, 01-AUG-2007
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ.
REMARK 100 THE RCSB ID CODE IS RCSB047871.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 03-AUG-2006
REMARK 200  TEMPERATURE           (KELVIN) : 290
REMARK 200  PH                             : 4.6
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : N
REMARK 200  RADIATION SOURCE               : ROTATING ANODE
REMARK 200  BEAMLINE                       : NULL
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-007
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : OSMIC VARIMAX
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : MAR SCANNER 345 MM PLATE
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MOSFLM
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 13027
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.900
REMARK 200  RESOLUTION RANGE LOW       (A) : 28.340
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0
REMARK 200  DATA REDUNDANCY                : 8.200
REMARK 200  R MERGE                    (I) : 0.06200
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 10.7000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.90
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.00
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 8.10
REMARK 200  R MERGE FOR SHELL          (I) : 0.33900
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 5.800
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: PDB ENTRY 1CEX
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 33.27
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 1.84
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 28% PEG 4000, 0.1M SODIUM ACETATE,
REMARK 280  PH 4.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 290K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 41 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,1/2+Z
REMARK 290       3555   1/2-Y,1/2+X,1/4+Z
REMARK 290       4555   1/2+Y,1/2-X,3/4+Z
REMARK 290       5555   1/2-X,1/2+Y,1/4-Z
REMARK 290       6555   1/2+X,1/2-Y,3/4-Z
REMARK 290       7555   Y,X,-Z
REMARK 290       8555   -Y,-X,1/2-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       43.20500
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       30.00000
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       30.00000
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       21.60250
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       30.00000
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       30.00000
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       64.80750
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       30.00000
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       30.00000
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       21.60250
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       30.00000
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       30.00000
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       64.80750
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       43.20500
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C  SSEQI
REMARK 465     ALA A    24
REMARK 465     MET A    25
REMARK 465     ALA A    26
REMARK 465     ILE A    27
REMARK 465     SER A    28
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS(M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     GLY A 224    CA   C    O
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    THR A  58      -13.00     83.45
REMARK 500    SER A  66     -105.52   -124.07
REMARK 500    TYR A  93       93.17    -68.73
REMARK 500    SER A 136     -119.45     52.94
REMARK 500    TYR A 165      107.17    -56.66
REMARK 500    VAL A 213      -71.93   -123.80
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 3DD5   RELATED DB: PDB
REMARK 900 E600-CUTINASE COMPLEX
REMARK 900 RELATED ID: 3DEA   RELATED DB: PDB
REMARK 900 PETFP-CUTINASE COMPLEX
REMARK 999
REMARK 999 SEQUENCE
REMARK 999 THE SEQUENCE IS BASED ON REFERENCE 2 IN THE DATABASE,
REMARK 999 CUTI_COLGL.
DBREF  3DCN A   31   224  UNP    P11373   CUTI_COLGL      31    224
SEQADV 3DCN ALA A   24  UNP  P11373              EXPRESSION TAG
SEQADV 3DCN MET A   25  UNP  P11373              EXPRESSION TAG
SEQADV 3DCN ALA A   26  UNP  P11373              EXPRESSION TAG
SEQADV 3DCN ILE A   27  UNP  P11373              EXPRESSION TAG
SEQADV 3DCN SER A   28  UNP  P11373              EXPRESSION TAG
SEQADV 3DCN ASP A   29  UNP  P11373              EXPRESSION TAG
SEQADV 3DCN PRO A   30  UNP  P11373              EXPRESSION TAG
SEQRES   1 A  201  ALA MET ALA ILE SER ASP PRO GLN SER SER THR ARG ASN
SEQRES   2 A  201  GLU LEU GLU THR GLY SER SER SER ALA CYS PRO LYS VAL
SEQRES   3 A  201  ILE TYR ILE PHE ALA ARG ALA SER THR GLU PRO GLY ASN
SEQRES   4 A  201  MET GLY ILE SER ALA GLY PRO ILE VAL ALA ASP ALA LEU
SEQRES   5 A  201  GLU ARG ILE TYR GLY ALA ASN ASP VAL TRP VAL GLN GLY
SEQRES   6 A  201  VAL GLY GLY PRO TYR LEU ALA ASP LEU ALA SER ASN PHE
SEQRES   7 A  201  LEU PRO ASP GLY THR SER SER ALA ALA ILE ASN GLU ALA
SEQRES   8 A  201  ARG ARG LEU PHE THR LEU ALA ASN THR LYS CYS PRO ASN
SEQRES   9 A  201  ALA ALA ILE VAL SER GLY GLY TYR SER GLN GLY THR ALA
SEQRES  10 A  201  VAL MET ALA GLY SER ILE SER GLY LEU SER THR THR ILE
SEQRES  11 A  201  LYS ASN GLN ILE LYS GLY VAL VAL LEU PHE GLY TYR THR
SEQRES  12 A  201  LYS ASN LEU GLN ASN LEU GLY ARG ILE PRO ASN PHE GLU
SEQRES  13 A  201  THR SER LYS THR GLU VAL TYR CYS ASP ILE ALA ASP ALA
SEQRES  14 A  201  VAL CYS TYR GLY THR LEU PHE ILE LEU PRO ALA HIS PHE
SEQRES  15 A  201  LEU TYR GLN THR ASP ALA ALA VAL ALA ALA PRO ARG PHE
SEQRES  16 A  201  LEU GLN ALA ARG ILE GLY
FORMUL   2  HOH   *149(H2 O)
HELIX    1   1 SER A   66  GLY A   80  1                                  15
HELIX    2   2 LEU A   97  LEU A  102  5                                   6
HELIX    3   3 SER A  107  CYS A  125  1                                  19
HELIX    4   4 SER A  136  SER A  147  1                                  12
HELIX    5   5 SER A  150  GLN A  156  1                                   7
HELIX    6   6 GLU A  179  SER A  181  5                                   3
HELIX    7   7 ASP A  191  GLY A  196  5                                   6
HELIX    8   8 TYR A  207  VAL A  213  1                                   7
HELIX    9   9 VAL A  213  ALA A  221  1                                   9
SHEET    1   A 5 VAL A  84  GLY A  88  0
SHEET    2   A 5 VAL A  49  ALA A  54  1  N  TYR A  51   O  TRP A  85
SHEET    3   A 5 ALA A 129  TYR A 135  1  O  ALA A 129   N  ILE A  50
SHEET    4   A 5 ILE A 157  PHE A 163  1  O  LYS A 158   N  ILE A 130
SHEET    5   A 5 THR A 183  TYR A 186  1  O  GLU A 184   N  VAL A 160
SSBOND   1 CYS A   46    CYS A  125                        1555   1555    2.08
SSBOND   2 CYS A  187    CYS A  194                        1555   1555    2.01
CRYST1   60.000   60.000   86.410  90.00  90.00  90.00 P 41 21 2     8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.016664  0.000000  0.000000        0.00000
SCALE2      0.000000  0.016664  0.000000        0.00000
SCALE3      0.000000  0.000000  0.011573        0.00000
TER    1447      GLY A 224
MASTER      295    0    0    9    5    0    0    6 1595    1    4   16
END