longtext: 3GZJ-pdb

content
HEADER    HYDROLASE                               07-APR-09   3GZJ
TITLE     CRYSTAL STRUCTURE OF POLYNEURIDINE ALDEHYDE ESTERASE
TITLE    2 COMPLEXED WITH 16-EPI-VELLOSIMINE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: POLYNEURIDINE-ALDEHYDE ESTERASE;
COMPND   3 CHAIN: A, B, C, D, E;
COMPND   4 EC: 3.1.1.78;
COMPND   5 ENGINEERED: YES;
COMPND   6 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: RAUVOLFIA SERPENTINA;
SOURCE   3 ORGANISM_COMMON: DEVILPEPPER;
SOURCE   4 ORGANISM_TAXID: 4060;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: M15;
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PREP4
KEYWDS    HYDROLASE SUPERFAMILY, ALKALOID METABOLISM, HYDROLASE,
KEYWDS   2 SERINE ESTERASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    L.YANG,M.HILL,M.WANG,S.PANJIKAR,J.STOECKIGT
REVDAT   1   18-AUG-09 3GZJ    0
JRNL        AUTH   L.YANG,M.HILL,M.WANG,S.PANJIKAR,J.STOCKIGT
JRNL        TITL   STRUCTURAL BASIS AND ENZYMATIC MECHANISM OF THE
JRNL        TITL 2 BIOSYNTHESIS OF C9- FROM C10-MONOTERPENOID INDOLE
JRNL        TITL 3 ALKALOIDS
JRNL        REF    ANGEW.CHEM.INT.ED.ENGL.       V.  48  5211 2009
JRNL        REFN                   ISSN 1433-7851
JRNL        PMID   19496101
JRNL        DOI    10.1002/ANIE.200900150
REMARK   1
REMARK   2
REMARK   2 RESOLUTION.    2.19 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.2.0019
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.19
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 20.00
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.2
REMARK   3   NUMBER OF REFLECTIONS             : 66881
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.212
REMARK   3   R VALUE            (WORKING SET) : 0.209
REMARK   3   FREE R VALUE                     : 0.278
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.100
REMARK   3   FREE R VALUE TEST SET COUNT      : 3407
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.19
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.25
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 4198
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 87.44
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2310
REMARK   3   BIN FREE R VALUE SET COUNT          : 230
REMARK   3   BIN FREE R VALUE                    : 0.3420
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 9985
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 88
REMARK   3   SOLVENT ATOMS            : 245
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 44.60
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 27.85
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : -2.05000
REMARK   3    B22 (A**2) : 3.26000
REMARK   3    B33 (A**2) : -0.90000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.61000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.321
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.253
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.201
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 16.041
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.940
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.898
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A): 10343 ; 0.018 ; 0.022
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 13968 ; 1.841 ; 1.988
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  1265 ; 6.814 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   430 ;35.778 ;24.651
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1845 ;18.159 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    35 ;16.221 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1486 ; 0.128 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  7734 ; 0.007 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  5227 ; 0.237 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  6998 ; 0.316 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   505 ; 0.177 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):   145 ; 0.360 ; 0.200
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):    19 ; 0.337 ; 0.200
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  6542 ; 0.923 ; 1.500
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2): 10215 ; 1.427 ; 2.000
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  4378 ; 2.491 ; 3.000
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  3753 ; 3.613 ; 4.500
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : 1
REMARK   3
REMARK   3  NCS GROUP NUMBER               : 1
REMARK   3     CHAIN NAMES                    : E B
REMARK   3     NUMBER OF COMPONENTS NCS GROUP : 4
REMARK   3       COMPONENT C  SSSEQI  TO  C   SSSEQI   CODE
REMARK   3           1     E    114       E     136      1
REMARK   3           1     B    114       B     136      1
REMARK   3           2     E    146       E     153      1
REMARK   3           2     B    146       B     153      1
REMARK   3           3     E    189       E     207      1
REMARK   3           3     B    189       B     207      1
REMARK   3           4     E    221       E     235      1
REMARK   3           4     B    221       B     235      1
REMARK   3                   GROUP CHAIN        COUNT   RMS     WEIGHT
REMARK   3   TIGHT POSITIONAL   1    E    (A):    524 ; 0.080 ; 0.050
REMARK   3   TIGHT THERMAL      1    E (A**2):    524 ; 0.310 ; 0.500
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE
REMARK   3  RIDING POSITIONS
REMARK   4
REMARK   4 3GZJ COMPLIES WITH FORMAT V. 3.20, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 10-APR-09.
REMARK 100 THE RCSB ID CODE IS RCSB052502.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 18-NOV-07
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 6.3
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SLS
REMARK 200  BEAMLINE                       : X06SA
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.000
REMARK 200  MONOCHROMATOR                  : SI(111)
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : PSI PILATUS 6M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XDS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 66881
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.190
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.2
REMARK 200  DATA REDUNDANCY                : 3.200
REMARK 200  R MERGE                    (I) : 0.07700
REMARK 200  R SYM                      (I) : 0.07700
REMARK 200   FOR THE DATA SET  : 10.9000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.19
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.32
REMARK 200  COMPLETENESS FOR SHELL     (%) : 93.9
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.00
REMARK 200  R MERGE FOR SHELL          (I) : 0.42500
REMARK 200  R SYM FOR SHELL            (I) : 0.42500
REMARK 200   FOR SHELL         : 2.200
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: 2WFL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 46.52
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.30
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: PEG 3350, 0.20M BIS-TRIS, 0.25M
REMARK 280  LIS2SO4, PH 6.3, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE
REMARK 280  296K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y,-Z
REMARK 290       3555   X+1/2,Y+1/2,Z
REMARK 290       4555   -X+1/2,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       85.60500
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       22.30500
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       85.60500
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       22.30500
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4, 5
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 5
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: E
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     ALA A     7
REMARK 465     LYS A     8
REMARK 465     GLN A     9
REMARK 465     SER A   264
REMARK 465     ALA B     7
REMARK 465     LYS B     8
REMARK 465     GLN B     9
REMARK 465     SER B   264
REMARK 465     ALA C     7
REMARK 465     LYS C     8
REMARK 465     GLN C     9
REMARK 465     SER C   264
REMARK 465     ALA D     7
REMARK 465     LYS D     8
REMARK 465     GLN D     9
REMARK 465     SER D   264
REMARK 465     ALA E     7
REMARK 465     LYS E     8
REMARK 465     GLN E     9
REMARK 465     SER E   264
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OG   SER D    87     CAF  EVS D  1000              1.42
REMARK 500   OG   SER C    87     CAF  EVS C  1000              1.45
REMARK 500   OG   SER A    87     CAF  EVS A  1000              1.46
REMARK 500   OG   SER B    87     CAF  EVS B  1000              1.48
REMARK 500   O    HOH A   295     O    HOH A   313              1.91
REMARK 500   CA   GLY C    49     O    HOH C   318              1.96
REMARK 500   OE2  GLU C   173     O    HOH C   308              2.10
REMARK 500   O    GLU A    79     NZ   LYS A   103              2.16
REMARK 500   O    HOH D   286     O    HOH D   308              2.19
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   NZ   LYS E   194     O    GLU E   201     4555     1.71
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    LYS B 196   CE    LYS B 196   NZ      0.174
REMARK 500    TRP E  26   CE3   TRP E  26   CZ3     0.104
REMARK 500    LYS E 196   CE    LYS E 196   NZ      0.163
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ASP A  44   CB  -  CG  -  OD1 ANGL. DEV. =   5.7 DEGREES
REMARK 500    LYS E 127   CD  -  CE  -  NZ  ANGL. DEV. =  21.2 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    LEU A  21     -156.53   -115.07
REMARK 500    ASP A  56        3.09    -67.17
REMARK 500    SER A  87     -132.99     48.44
REMARK 500    SER A 111       59.42     34.24
REMARK 500    LEU A 138     -121.89     61.34
REMARK 500    ARG A 202      -85.73   -109.92
REMARK 500    ASP A 243     -158.90    -96.76
REMARK 500    MET A 245       57.44   -103.44
REMARK 500    CYS B  20       14.18     57.61
REMARK 500    LEU B  21     -162.74   -121.74
REMARK 500    ALA B  73      -19.61    -48.24
REMARK 500    SER B  87     -124.21     39.13
REMARK 500    SER B 111       68.96     24.93
REMARK 500    LEU B 138     -119.34     57.71
REMARK 500    ASN B 150       66.55   -155.54
REMARK 500    ARG B 202      -72.69   -105.27
REMARK 500    LEU C  21     -168.48   -115.73
REMARK 500    ASP C  56        0.84    -66.17
REMARK 500    SER C  87     -121.53     44.03
REMARK 500    LEU C 138     -118.52     57.57
REMARK 500    PRO C 148       22.13    -63.69
REMARK 500    ASN C 150       67.02   -161.94
REMARK 500    ARG C 202      -74.48    -98.26
REMARK 500    PHE C 219       72.81   -152.32
REMARK 500    ASP C 243     -152.70    -87.87
REMARK 500    MET C 245       61.06   -115.98
REMARK 500    LEU D  21     -161.80   -116.78
REMARK 500    SER D  87     -120.22     46.81
REMARK 500    LEU D 138     -116.66     47.18
REMARK 500    PRO D 148        5.06    -66.21
REMARK 500    GLU D 149      -84.79    -98.43
REMARK 500    ARG D 202      -79.89   -105.06
REMARK 500    ASP D 243     -155.13    -89.95
REMARK 500    MET D 245       55.01   -108.09
REMARK 500    LEU E  21     -157.08   -120.44
REMARK 500    PRO E  52       -8.87    -59.41
REMARK 500    ASP E  56       40.56    -67.80
REMARK 500    GLU E  57       -2.33   -168.68
REMARK 500    SER E  74       58.70   -108.47
REMARK 500    SER E  87     -136.44     51.67
REMARK 500    PHE E  88      -14.69    -46.86
REMARK 500    LEU E 138     -102.24     55.48
REMARK 500    ASN E 150       59.82   -153.13
REMARK 500    ARG E 202      -70.28   -101.52
REMARK 500    MET E 245       56.36   -114.83
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CHIRAL CENTERS
REMARK 500
REMARK 500 UNEXPECTED CONFIGURATION OF THE FOLLOWING CHIRAL
REMARK 500 CENTER(S) USING IMPROPER CA--C--CB--N CHIRALITY
REMARK 500 M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (11X,I3,1X,A3,1X,A1,I4,A1,6X,F5.1,6X,A1,10X,A1,3X,A16)
REMARK 500
REMARK 500   M RES CSSEQI    IMPROPER   EXPECTED   FOUND DETAILS
REMARK 500     GLU B  66        24.7      L          L   OUTSIDE RANGE
REMARK 500     ASP C  63        16.2      L          L   OUTSIDE RANGE
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A 278        DISTANCE =  5.54 ANGSTROMS
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EVS A 1000
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EVS B 1000
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EVS C 1000
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EVS D 1000
DBREF  3GZJ A    7   264  UNP    Q9SE93   PNAE_RAUSE       7    264
DBREF  3GZJ B    7   264  UNP    Q9SE93   PNAE_RAUSE       7    264
DBREF  3GZJ C    7   264  UNP    Q9SE93   PNAE_RAUSE       7    264
DBREF  3GZJ D    7   264  UNP    Q9SE93   PNAE_RAUSE       7    264
DBREF  3GZJ E    7   264  UNP    Q9SE93   PNAE_RAUSE       7    264
SEQADV 3GZJ ALA A  244  UNP  Q9SE93    HIS   244 ENGINEERED
SEQADV 3GZJ ALA B  244  UNP  Q9SE93    HIS   244 ENGINEERED
SEQADV 3GZJ ALA C  244  UNP  Q9SE93    HIS   244 ENGINEERED
SEQADV 3GZJ ALA D  244  UNP  Q9SE93    HIS   244 ENGINEERED
SEQADV 3GZJ ALA E  244  UNP  Q9SE93    HIS   244 ENGINEERED
SEQRES   1 A  258  ALA LYS GLN GLN LYS HIS PHE VAL LEU VAL HIS GLY GLY
SEQRES   2 A  258  CYS LEU GLY ALA TRP ILE TRP TYR LYS LEU LYS PRO LEU
SEQRES   3 A  258  LEU GLU SER ALA GLY HIS LYS VAL THR ALA VAL ASP LEU
SEQRES   4 A  258  SER ALA ALA GLY ILE ASN PRO ARG ARG LEU ASP GLU ILE
SEQRES   5 A  258  HIS THR PHE ARG ASP TYR SER GLU PRO LEU MET GLU VAL
SEQRES   6 A  258  MET ALA SER ILE PRO PRO ASP GLU LYS VAL VAL LEU LEU
SEQRES   7 A  258  GLY HIS SER PHE GLY GLY MET SER LEU GLY LEU ALA MET
SEQRES   8 A  258  GLU THR TYR PRO GLU LYS ILE SER VAL ALA VAL PHE MET
SEQRES   9 A  258  SER ALA MET MET PRO ASP PRO ASN HIS SER LEU THR TYR
SEQRES  10 A  258  PRO PHE GLU LYS TYR ASN GLU LYS CYS PRO ALA ASP MET
SEQRES  11 A  258  MET LEU ASP SER GLN PHE SER THR TYR GLY ASN PRO GLU
SEQRES  12 A  258  ASN PRO GLY MET SER MET ILE LEU GLY PRO GLN PHE MET
SEQRES  13 A  258  ALA LEU LYS MET PHE GLN ASN CYS SER VAL GLU ASP LEU
SEQRES  14 A  258  GLU LEU ALA LYS MET LEU THR ARG PRO GLY SER LEU PHE
SEQRES  15 A  258  PHE GLN ASP LEU ALA LYS ALA LYS LYS PHE SER THR GLU
SEQRES  16 A  258  ARG TYR GLY SER VAL LYS ARG ALA TYR ILE PHE CYS ASN
SEQRES  17 A  258  GLU ASP LYS SER PHE PRO VAL GLU PHE GLN LYS TRP PHE
SEQRES  18 A  258  VAL GLU SER VAL GLY ALA ASP LYS VAL LYS GLU ILE LYS
SEQRES  19 A  258  GLU ALA ASP ALA MET GLY MET LEU SER GLN PRO ARG GLU
SEQRES  20 A  258  VAL CYS LYS CYS LEU LEU ASP ILE SER ASP SER
SEQRES   1 B  258  ALA LYS GLN GLN LYS HIS PHE VAL LEU VAL HIS GLY GLY
SEQRES   2 B  258  CYS LEU GLY ALA TRP ILE TRP TYR LYS LEU LYS PRO LEU
SEQRES   3 B  258  LEU GLU SER ALA GLY HIS LYS VAL THR ALA VAL ASP LEU
SEQRES   4 B  258  SER ALA ALA GLY ILE ASN PRO ARG ARG LEU ASP GLU ILE
SEQRES   5 B  258  HIS THR PHE ARG ASP TYR SER GLU PRO LEU MET GLU VAL
SEQRES   6 B  258  MET ALA SER ILE PRO PRO ASP GLU LYS VAL VAL LEU LEU
SEQRES   7 B  258  GLY HIS SER PHE GLY GLY MET SER LEU GLY LEU ALA MET
SEQRES   8 B  258  GLU THR TYR PRO GLU LYS ILE SER VAL ALA VAL PHE MET
SEQRES   9 B  258  SER ALA MET MET PRO ASP PRO ASN HIS SER LEU THR TYR
SEQRES  10 B  258  PRO PHE GLU LYS TYR ASN GLU LYS CYS PRO ALA ASP MET
SEQRES  11 B  258  MET LEU ASP SER GLN PHE SER THR TYR GLY ASN PRO GLU
SEQRES  12 B  258  ASN PRO GLY MET SER MET ILE LEU GLY PRO GLN PHE MET
SEQRES  13 B  258  ALA LEU LYS MET PHE GLN ASN CYS SER VAL GLU ASP LEU
SEQRES  14 B  258  GLU LEU ALA LYS MET LEU THR ARG PRO GLY SER LEU PHE
SEQRES  15 B  258  PHE GLN ASP LEU ALA LYS ALA LYS LYS PHE SER THR GLU
SEQRES  16 B  258  ARG TYR GLY SER VAL LYS ARG ALA TYR ILE PHE CYS ASN
SEQRES  17 B  258  GLU ASP LYS SER PHE PRO VAL GLU PHE GLN LYS TRP PHE
SEQRES  18 B  258  VAL GLU SER VAL GLY ALA ASP LYS VAL LYS GLU ILE LYS
SEQRES  19 B  258  GLU ALA ASP ALA MET GLY MET LEU SER GLN PRO ARG GLU
SEQRES  20 B  258  VAL CYS LYS CYS LEU LEU ASP ILE SER ASP SER
SEQRES   1 C  258  ALA LYS GLN GLN LYS HIS PHE VAL LEU VAL HIS GLY GLY
SEQRES   2 C  258  CYS LEU GLY ALA TRP ILE TRP TYR LYS LEU LYS PRO LEU
SEQRES   3 C  258  LEU GLU SER ALA GLY HIS LYS VAL THR ALA VAL ASP LEU
SEQRES   4 C  258  SER ALA ALA GLY ILE ASN PRO ARG ARG LEU ASP GLU ILE
SEQRES   5 C  258  HIS THR PHE ARG ASP TYR SER GLU PRO LEU MET GLU VAL
SEQRES   6 C  258  MET ALA SER ILE PRO PRO ASP GLU LYS VAL VAL LEU LEU
SEQRES   7 C  258  GLY HIS SER PHE GLY GLY MET SER LEU GLY LEU ALA MET
SEQRES   8 C  258  GLU THR TYR PRO GLU LYS ILE SER VAL ALA VAL PHE MET
SEQRES   9 C  258  SER ALA MET MET PRO ASP PRO ASN HIS SER LEU THR TYR
SEQRES  10 C  258  PRO PHE GLU LYS TYR ASN GLU LYS CYS PRO ALA ASP MET
SEQRES  11 C  258  MET LEU ASP SER GLN PHE SER THR TYR GLY ASN PRO GLU
SEQRES  12 C  258  ASN PRO GLY MET SER MET ILE LEU GLY PRO GLN PHE MET
SEQRES  13 C  258  ALA LEU LYS MET PHE GLN ASN CYS SER VAL GLU ASP LEU
SEQRES  14 C  258  GLU LEU ALA LYS MET LEU THR ARG PRO GLY SER LEU PHE
SEQRES  15 C  258  PHE GLN ASP LEU ALA LYS ALA LYS LYS PHE SER THR GLU
SEQRES  16 C  258  ARG TYR GLY SER VAL LYS ARG ALA TYR ILE PHE CYS ASN
SEQRES  17 C  258  GLU ASP LYS SER PHE PRO VAL GLU PHE GLN LYS TRP PHE
SEQRES  18 C  258  VAL GLU SER VAL GLY ALA ASP LYS VAL LYS GLU ILE LYS
SEQRES  19 C  258  GLU ALA ASP ALA MET GLY MET LEU SER GLN PRO ARG GLU
SEQRES  20 C  258  VAL CYS LYS CYS LEU LEU ASP ILE SER ASP SER
SEQRES   1 D  258  ALA LYS GLN GLN LYS HIS PHE VAL LEU VAL HIS GLY GLY
SEQRES   2 D  258  CYS LEU GLY ALA TRP ILE TRP TYR LYS LEU LYS PRO LEU
SEQRES   3 D  258  LEU GLU SER ALA GLY HIS LYS VAL THR ALA VAL ASP LEU
SEQRES   4 D  258  SER ALA ALA GLY ILE ASN PRO ARG ARG LEU ASP GLU ILE
SEQRES   5 D  258  HIS THR PHE ARG ASP TYR SER GLU PRO LEU MET GLU VAL
SEQRES   6 D  258  MET ALA SER ILE PRO PRO ASP GLU LYS VAL VAL LEU LEU
SEQRES   7 D  258  GLY HIS SER PHE GLY GLY MET SER LEU GLY LEU ALA MET
SEQRES   8 D  258  GLU THR TYR PRO GLU LYS ILE SER VAL ALA VAL PHE MET
SEQRES   9 D  258  SER ALA MET MET PRO ASP PRO ASN HIS SER LEU THR TYR
SEQRES  10 D  258  PRO PHE GLU LYS TYR ASN GLU LYS CYS PRO ALA ASP MET
SEQRES  11 D  258  MET LEU ASP SER GLN PHE SER THR TYR GLY ASN PRO GLU
SEQRES  12 D  258  ASN PRO GLY MET SER MET ILE LEU GLY PRO GLN PHE MET
SEQRES  13 D  258  ALA LEU LYS MET PHE GLN ASN CYS SER VAL GLU ASP LEU
SEQRES  14 D  258  GLU LEU ALA LYS MET LEU THR ARG PRO GLY SER LEU PHE
SEQRES  15 D  258  PHE GLN ASP LEU ALA LYS ALA LYS LYS PHE SER THR GLU
SEQRES  16 D  258  ARG TYR GLY SER VAL LYS ARG ALA TYR ILE PHE CYS ASN
SEQRES  17 D  258  GLU ASP LYS SER PHE PRO VAL GLU PHE GLN LYS TRP PHE
SEQRES  18 D  258  VAL GLU SER VAL GLY ALA ASP LYS VAL LYS GLU ILE LYS
SEQRES  19 D  258  GLU ALA ASP ALA MET GLY MET LEU SER GLN PRO ARG GLU
SEQRES  20 D  258  VAL CYS LYS CYS LEU LEU ASP ILE SER ASP SER
SEQRES   1 E  258  ALA LYS GLN GLN LYS HIS PHE VAL LEU VAL HIS GLY GLY
SEQRES   2 E  258  CYS LEU GLY ALA TRP ILE TRP TYR LYS LEU LYS PRO LEU
SEQRES   3 E  258  LEU GLU SER ALA GLY HIS LYS VAL THR ALA VAL ASP LEU
SEQRES   4 E  258  SER ALA ALA GLY ILE ASN PRO ARG ARG LEU ASP GLU ILE
SEQRES   5 E  258  HIS THR PHE ARG ASP TYR SER GLU PRO LEU MET GLU VAL
SEQRES   6 E  258  MET ALA SER ILE PRO PRO ASP GLU LYS VAL VAL LEU LEU
SEQRES   7 E  258  GLY HIS SER PHE GLY GLY MET SER LEU GLY LEU ALA MET
SEQRES   8 E  258  GLU THR TYR PRO GLU LYS ILE SER VAL ALA VAL PHE MET
SEQRES   9 E  258  SER ALA MET MET PRO ASP PRO ASN HIS SER LEU THR TYR
SEQRES  10 E  258  PRO PHE GLU LYS TYR ASN GLU LYS CYS PRO ALA ASP MET
SEQRES  11 E  258  MET LEU ASP SER GLN PHE SER THR TYR GLY ASN PRO GLU
SEQRES  12 E  258  ASN PRO GLY MET SER MET ILE LEU GLY PRO GLN PHE MET
SEQRES  13 E  258  ALA LEU LYS MET PHE GLN ASN CYS SER VAL GLU ASP LEU
SEQRES  14 E  258  GLU LEU ALA LYS MET LEU THR ARG PRO GLY SER LEU PHE
SEQRES  15 E  258  PHE GLN ASP LEU ALA LYS ALA LYS LYS PHE SER THR GLU
SEQRES  16 E  258  ARG TYR GLY SER VAL LYS ARG ALA TYR ILE PHE CYS ASN
SEQRES  17 E  258  GLU ASP LYS SER PHE PRO VAL GLU PHE GLN LYS TRP PHE
SEQRES  18 E  258  VAL GLU SER VAL GLY ALA ASP LYS VAL LYS GLU ILE LYS
SEQRES  19 E  258  GLU ALA ASP ALA MET GLY MET LEU SER GLN PRO ARG GLU
SEQRES  20 E  258  VAL CYS LYS CYS LEU LEU ASP ILE SER ASP SER
HET    EVS  A1000      22
HET    EVS  B1000      22
HET    EVS  C1000      22
HET    EVS  D1000      22
HETNAM     EVS 16-EPI-VELLOSIMINE
FORMUL   6  EVS    4(C19 H20 N2 O)
FORMUL  10  HOH   *245(H2 O)
HELIX    1   1 GLY A   22  TYR A   27  5                                   6
HELIX    2   2 LYS A   28  ALA A   36  1                                   9
HELIX    3   3 ARG A   54  ILE A   58  5                                   5
HELIX    4   4 THR A   60  SER A   74  1                                  15
HELIX    5   5 PHE A   88  TYR A  100  1                                  13
HELIX    6   6 THR A  122  CYS A  132  1                                  11
HELIX    7   7 GLY A  158  MET A  166  1                                   9
HELIX    8   8 SER A  171  THR A  182  1                                  12
HELIX    9   9 PHE A  188  ALA A  193  1                                   6
HELIX   10  10 ARG A  202  VAL A  206  5                                   5
HELIX   11  11 PRO A  220  GLY A  232  1                                  13
HELIX   12  12 MET A  245  GLN A  250  1                                   6
HELIX   13  13 GLN A  250  ASP A  263  1                                  14
HELIX   14  14 GLY B   22  TYR B   27  5                                   6
HELIX   15  15 LEU B   29  ALA B   36  1                                   8
HELIX   16  16 ARG B   54  ILE B   58  5                                   5
HELIX   17  17 THR B   60  SER B   74  1                                  15
HELIX   18  18 PHE B   88  TYR B  100  1                                  13
HELIX   19  19 THR B  122  PRO B  124  5                                   3
HELIX   20  20 PHE B  125  CYS B  132  1                                   8
HELIX   21  21 GLY B  158  MET B  166  1                                   9
HELIX   22  22 SER B  171  THR B  182  1                                  12
HELIX   23  23 PHE B  188  LYS B  194  1                                   7
HELIX   24  24 ARG B  202  VAL B  206  5                                   5
HELIX   25  25 PRO B  220  VAL B  231  1                                  12
HELIX   26  26 MET B  245  GLN B  250  1                                   6
HELIX   27  27 GLN B  250  ASP B  263  1                                  14
HELIX   28  28 GLY C   22  TYR C   27  5                                   6
HELIX   29  29 LYS C   28  ALA C   36  1                                   9
HELIX   30  30 ARG C   54  ILE C   58  5                                   5
HELIX   31  31 THR C   60  ILE C   75  1                                  16
HELIX   32  32 PHE C   88  TYR C  100  1                                  13
HELIX   33  33 THR C  122  PRO C  124  5                                   3
HELIX   34  34 PHE C  125  CYS C  132  1                                   8
HELIX   35  35 GLY C  158  MET C  166  1                                   9
HELIX   36  36 SER C  171  MET C  180  1                                  10
HELIX   37  37 PHE C  188  ALA C  195  1                                   8
HELIX   38  38 ARG C  202  VAL C  206  5                                   5
HELIX   39  39 PRO C  220  VAL C  231  1                                  12
HELIX   40  40 MET C  245  GLN C  250  1                                   6
HELIX   41  41 GLN C  250  ASP C  263  1                                  14
HELIX   42  42 GLY D   22  TYR D   27  5                                   6
HELIX   43  43 LYS D   28  ALA D   36  1                                   9
HELIX   44  44 ARG D   54  ILE D   58  5                                   5
HELIX   45  45 THR D   60  SER D   74  1                                  15
HELIX   46  46 PHE D   88  TYR D  100  1                                  13
HELIX   47  47 THR D  122  CYS D  132  1                                  11
HELIX   48  48 GLY D  158  MET D  166  1                                   9
HELIX   49  49 SER D  171  THR D  182  1                                  12
HELIX   50  50 PHE D  188  ALA D  195  1                                   8
HELIX   51  51 ARG D  202  VAL D  206  5                                   5
HELIX   52  52 PRO D  220  GLY D  232  1                                  13
HELIX   53  53 MET D  245  GLN D  250  1                                   6
HELIX   54  54 GLN D  250  ASP D  263  1                                  14
HELIX   55  55 GLY E   22  TYR E   27  5                                   6
HELIX   56  56 LYS E   28  ALA E   36  1                                   9
HELIX   57  57 THR E   60  SER E   65  1                                   6
HELIX   58  58 SER E   65  SER E   74  1                                  10
HELIX   59  59 PHE E   88  TYR E  100  1                                  13
HELIX   60  60 THR E  122  PRO E  124  5                                   3
HELIX   61  61 PHE E  125  CYS E  132  1                                   8
HELIX   62  62 PRO E  133  LEU E  138  5                                   6
HELIX   63  63 GLY E  158  MET E  166  1                                   9
HELIX   64  64 SER E  171  THR E  182  1                                  12
HELIX   65  65 PHE E  188  LYS E  194  1                                   7
HELIX   66  66 ARG E  202  VAL E  206  5                                   5
HELIX   67  67 PRO E  220  VAL E  231  1                                  12
HELIX   68  68 MET E  245  GLN E  250  1                                   6
HELIX   69  69 GLN E  250  SER E  262  1                                  13
SHEET    1   A 6 LYS A  39  ALA A  42  0
SHEET    2   A 6 HIS A  12  VAL A  16  1  N  LEU A  15   O  THR A  41
SHEET    3   A 6 VAL A  81  SER A  87  1  O  VAL A  82   N  VAL A  14
SHEET    4   A 6 ILE A 104  ALA A 112  1  O  VAL A 108   N  LEU A  83
SHEET    5   A 6 ARG A 208  CYS A 213  1  O  ALA A 209   N  ALA A 107
SHEET    6   A 6 LYS A 235  ILE A 239  1  O  LYS A 237   N  PHE A 212
SHEET    1   B 2 GLN A 141  TYR A 145  0
SHEET    2   B 2 GLY A 152  ILE A 156 -1  O  GLY A 152   N  TYR A 145
SHEET    1   C 6 LYS B  39  ALA B  42  0
SHEET    2   C 6 HIS B  12  VAL B  16  1  N  LEU B  15   O  THR B  41
SHEET    3   C 6 VAL B  81  SER B  87  1  O  VAL B  82   N  VAL B  14
SHEET    4   C 6 ILE B 104  ALA B 112  1  O  VAL B 108   N  LEU B  83
SHEET    5   C 6 LYS B 207  CYS B 213  1  O  ALA B 209   N  ALA B 107
SHEET    6   C 6 LYS B 235  ILE B 239  1  O  LYS B 237   N  PHE B 212
SHEET    1   D 2 GLN B 141  GLY B 146  0
SHEET    2   D 2 PRO B 151  ILE B 156 -1  O  SER B 154   N  SER B 143
SHEET    1   E 6 LYS C  39  ALA C  42  0
SHEET    2   E 6 HIS C  12  VAL C  16  1  N  LEU C  15   O  THR C  41
SHEET    3   E 6 VAL C  81  SER C  87  1  O  VAL C  82   N  HIS C  12
SHEET    4   E 6 ILE C 104  ALA C 112  1  O  VAL C 108   N  LEU C  83
SHEET    5   E 6 LYS C 207  CYS C 213  1  O  LYS C 207   N  ALA C 107
SHEET    6   E 6 LYS C 235  ILE C 239  1  O  LYS C 237   N  PHE C 212
SHEET    1   F 2 GLN C 141  TYR C 145  0
SHEET    2   F 2 GLY C 152  ILE C 156 -1  O  GLY C 152   N  TYR C 145
SHEET    1   G 6 LYS D  39  VAL D  43  0
SHEET    2   G 6 HIS D  12  VAL D  16  1  N  PHE D  13   O  LYS D  39
SHEET    3   G 6 VAL D  81  SER D  87  1  O  LEU D  84   N  VAL D  16
SHEET    4   G 6 ILE D 104  ALA D 112  1  O  MET D 110   N  GLY D  85
SHEET    5   G 6 LYS D 207  CYS D 213  1  O  ILE D 211   N  PHE D 109
SHEET    6   G 6 LYS D 235  ILE D 239  1  O  ILE D 239   N  PHE D 212
SHEET    1   H 2 GLN D 141  TYR D 145  0
SHEET    2   H 2 GLY D 152  ILE D 156 -1  O  SER D 154   N  SER D 143
SHEET    1   I 6 LYS E  39  VAL E  43  0
SHEET    2   I 6 HIS E  12  VAL E  16  1  N  PHE E  13   O  THR E  41
SHEET    3   I 6 VAL E  81  SER E  87  1  O  VAL E  82   N  VAL E  14
SHEET    4   I 6 ILE E 104  ALA E 112  1  O  MET E 110   N  GLY E  85
SHEET    5   I 6 ARG E 208  CYS E 213  1  O  ALA E 209   N  PHE E 109
SHEET    6   I 6 LYS E 235  ILE E 239  1  O  ILE E 239   N  PHE E 212
SHEET    1   J 3 GLN E 141  SER E 143  0
SHEET    2   J 3 SER E 154  ILE E 156 -1  O  SER E 154   N  SER E 143
SHEET    3   J 3 GLY E 185  SER E 186 -1  O  GLY E 185   N  MET E 155
SITE     1 AC1 10 HOH A   3  GLY A  19  SER A  87  PHE A  88
SITE     2 AC1 10 MET A 113  PRO A 124  TYR A 128  MET A 155
SITE     3 AC1 10 LEU A 187  HOH A 270
SITE     1 AC2 10 GLY B  19  SER B  87  PHE B  88  MET B 113
SITE     2 AC2 10 PRO B 124  PHE B 125  TYR B 128  MET B 137
SITE     3 AC2 10 LEU B 187  HOH B 290
SITE     1 AC3 12 GLY C  19  SER C  87  PHE C  88  MET C 113
SITE     2 AC3 12 PHE C 125  TYR C 128  MET C 155  LEU C 157
SITE     3 AC3 12 LEU C 187  HOH C 270  HOH C 289  HOH D 301
SITE     1 AC4 11 GLY D  19  SER D  87  PHE D  88  MET D 113
SITE     2 AC4 11 PRO D 124  PHE D 125  TYR D 128  MET D 137
SITE     3 AC4 11 LEU D 157  HOH D 270  HOH D 282
CRYST1  171.210   44.610  180.410  90.00 104.44  90.00 C 1 2 1      20
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.005841  0.000000  0.001504        0.00000
SCALE2      0.000000  0.022416  0.000000        0.00000
SCALE3      0.000000  0.000000  0.005724        0.00000
TER    1998      ASP A 263
TER    3996      ASP B 263
TER    5994      ASP C 263
TER    7992      ASP D 263
TER    9990      ASP E 263
MASTER      485    0    4   69   41    0   12    610318    5   88  100
END