longtext: 3NGM-pdb

content
HEADER    HYDROLASE                               12-JUN-10   3NGM
TITLE     CRYSTAL STRUCTURE OF LIPASE FROM GIBBERELLA ZEAE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: EXTRACELLULAR LIPASE;
COMPND   3 CHAIN: A, B, C, D;
COMPND   4 FRAGMENT: RESIDUES 1-319;
COMPND   5 EC: 3.1.1.3;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: GIBBERELLA ZEAE;
SOURCE   3 ORGANISM_TAXID: 5518;
SOURCE   4 GENE: FGL1;
SOURCE   5 EXPRESSION_SYSTEM: PICHIA PASTORIS;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 4922;
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: KM71;
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PLIZG7
KEYWDS    SECRET LIPASE, GIBBERELLA ZEAE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    Z.Y.LOU,M.LI,Y.N.SUN,Y.LIU,Z.LIU,Z.H.RAO
REVDAT   1   25-MAY-11 3NGM    0
JRNL        AUTH   Z.Y.LOU,M.LI,Y.N.SUN,Y.LIU,Z.LIU,W.P.WU,Z.H.RAO
JRNL        TITL   CRYSTAL STRUCTURE OF A SECRETED LIPASE FROM GIBBERELLA ZEAE
JRNL        TITL 2 REVEALS A NOVEL "DOUBLE-LOCK" MECHANISM
JRNL        REF    PROTEIN CELL                  V.   1   760 2010
JRNL        REFN                   ISSN 1674-800X
JRNL        PMID   21203917
JRNL        DOI    10.1007/S13238-010-0094-Y
REMARK   2
REMARK   2 RESOLUTION.    2.80 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : CNS 1.1
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,
REMARK   3               : READ,RICE,SIMONSON,WARREN
REMARK   3
REMARK   3  REFINEMENT TARGET : ENGH & HUBER
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.80
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 50.00
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : NULL
REMARK   3   NUMBER OF REFLECTIONS             : 29217
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE            (WORKING SET) : 0.238
REMARK   3   FREE R VALUE                     : 0.264
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : NULL
REMARK   3   FREE R VALUE TEST SET COUNT      : 2906
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL
REMARK   3   BIN R VALUE           (WORKING SET) : NULL
REMARK   3   BIN FREE R VALUE                    : NULL
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 8792
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 0
REMARK   3   SOLVENT ATOMS            : 218
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL
REMARK   3   ESD FROM SIGMAA              (A) : NULL
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL
REMARK   3
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.
REMARK   3   BOND LENGTHS                 (A) : 1.097
REMARK   3   BOND ANGLES            (DEGREES) : 0.01
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : NULL
REMARK   3   IMPROPER ANGLES        (DEGREES) : NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL MODEL : NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELING.
REMARK   3   METHOD USED : NULL
REMARK   3   KSOL        : NULL
REMARK   3   BSOL        : NULL
REMARK   3
REMARK   3  NCS MODEL : NULL
REMARK   3
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  PARAMETER FILE  1  : NULL
REMARK   3  TOPOLOGY FILE  1   : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 3NGM COMPLIES WITH FORMAT V. 3.20, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 25-JUN-10.
REMARK 100 THE RCSB ID CODE IS RCSB059803.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 03-JAN-08
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 5.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : N
REMARK 200  RADIATION SOURCE               : ROTATING ANODE
REMARK 200  BEAMLINE                       : NULL
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-007
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : OSMIC MIRROR
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : MAR SCANNER 345 MM PLATE
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 34080
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.800
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.0
REMARK 200  DATA REDUNDANCY                : NULL
REMARK 200  R MERGE                    (I) : 0.10100
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : NULL
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: 1EIN
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 52.39
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.58
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: PH 5.5, VAPOR DIFFUSION, HANGING DROP,
REMARK 280  TEMPERATURE 291K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       39.20850
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       97.90100
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       45.50050
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       97.90100
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       39.20850
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       45.50050
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     ALA A   269
REMARK 465     GLY A   270
REMARK 465     GLY A   271
REMARK 465     ILE A   272
REMARK 465     SER A   273
REMARK 465     TRP A   274
REMARK 465     ARG A   275
REMARK 465     ARG A   276
REMARK 465     TYR A   277
REMARK 465     ARG A   278
REMARK 465     SER A   279
REMARK 465     ALA A   280
REMARK 465     LYS A   281
REMARK 465     ARG A   282
REMARK 465     GLU A   283
REMARK 465     SER A   284
REMARK 465     ILE A   285
REMARK 465     SER A   286
REMARK 465     GLU A   287
REMARK 465     ARG A   288
REMARK 465     ALA A   289
REMARK 465     SER A   319
REMARK 465     ALA B   269
REMARK 465     GLY B   270
REMARK 465     GLY B   271
REMARK 465     ILE B   272
REMARK 465     SER B   273
REMARK 465     TRP B   274
REMARK 465     ARG B   275
REMARK 465     ARG B   276
REMARK 465     TYR B   277
REMARK 465     ARG B   278
REMARK 465     SER B   279
REMARK 465     ALA B   280
REMARK 465     LYS B   281
REMARK 465     ARG B   282
REMARK 465     GLU B   283
REMARK 465     SER B   284
REMARK 465     ILE B   285
REMARK 465     SER B   286
REMARK 465     GLU B   287
REMARK 465     ARG B   288
REMARK 465     ALA B   289
REMARK 465     SER B   319
REMARK 465     ALA C   269
REMARK 465     GLY C   270
REMARK 465     GLY C   271
REMARK 465     ILE C   272
REMARK 465     SER C   273
REMARK 465     TRP C   274
REMARK 465     ARG C   275
REMARK 465     ARG C   276
REMARK 465     TYR C   277
REMARK 465     ARG C   278
REMARK 465     SER C   279
REMARK 465     ALA C   280
REMARK 465     LYS C   281
REMARK 465     ARG C   282
REMARK 465     GLU C   283
REMARK 465     SER C   284
REMARK 465     ILE C   285
REMARK 465     SER C   286
REMARK 465     GLU C   287
REMARK 465     ARG C   288
REMARK 465     ALA C   289
REMARK 465     SER C   319
REMARK 465     ALA D   269
REMARK 465     GLY D   270
REMARK 465     GLY D   271
REMARK 465     ILE D   272
REMARK 465     SER D   273
REMARK 465     TRP D   274
REMARK 465     ARG D   275
REMARK 465     ARG D   276
REMARK 465     TYR D   277
REMARK 465     ARG D   278
REMARK 465     SER D   279
REMARK 465     ALA D   280
REMARK 465     LYS D   281
REMARK 465     ARG D   282
REMARK 465     GLU D   283
REMARK 465     SER D   284
REMARK 465     ILE D   285
REMARK 465     SER D   286
REMARK 465     GLU D   287
REMARK 465     ARG D   288
REMARK 465     ALA D   289
REMARK 465     SER D   319
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   NH2  ARG B   202     O    HOH B   350              2.00
REMARK 500   N    THR A   292     O    HOH A   327              2.02
REMARK 500   O    LYS C    59     O    HOH C   356              2.03
REMARK 500   CB   CYS B   237     O    HOH B   321              2.06
REMARK 500   N    ILE B    83     O    HOH B   358              2.06
REMARK 500   N    ASN B   242     O    HOH B   332              2.06
REMARK 500   OG   SER B   119     O    HOH B   345              2.07
REMARK 500   CB   SER B    26     O    HOH B   320              2.07
REMARK 500   N    ARG C    86     O    HOH C   352              2.08
REMARK 500   O    CYS B    36     O    HOH B   334              2.09
REMARK 500   OD1  ASN A   133     O    HOH A   344              2.11
REMARK 500   OE1  GLU C    74     O    HOH C   340              2.12
REMARK 500   O    GLN C   244     O    HOH C   328              2.12
REMARK 500   N    VAL D   174     O    HOH D   335              2.14
REMARK 500   O    GLY D   223     O    HOH D   323              2.14
REMARK 500   O    HOH C   322     O    HOH C   358              2.14
REMARK 500   OG1  THR B   162     O    HOH B   357              2.15
REMARK 500   OD2  ASP C    98     O    HOH C   323              2.16
REMARK 500   NE2  GLN D    45     O    HOH D   344              2.16
REMARK 500   N    CYS B    41     O    HOH B   344              2.17
REMARK 500   CB   ASN B   242     O    HOH B   332              2.17
REMARK 500   CA   THR C   102     O    HOH C   332              2.17
REMARK 500   OG   SER B   316     O    HOH B   333              2.18
REMARK 500   O    HOH B   354     O    HOH C   334              2.18
REMARK 500   SG   CYS D   245     O    HOH D   354              2.18
REMARK 500   O    ALA C    22     OG   SER C    26              2.18
REMARK 500   CB   CYS C   237     O    HOH C   336              2.18
REMARK 500   N    ALA A   263     O    HOH A   352              2.18
REMARK 500   O    VAL C   193     O    HOH C   360              2.19
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    GLY B 223   C     GLY B 223   O      -0.097
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    GLY A 224   N   -  CA  -  C   ANGL. DEV. = -16.4 DEGREES
REMARK 500    CYS A 267   CA  -  CB  -  SG  ANGL. DEV. =   7.4 DEGREES
REMARK 500    CYS A 267   N   -  CA  -  C   ANGL. DEV. =  16.6 DEGREES
REMARK 500    SER A 268   C   -  N   -  CA  ANGL. DEV. =  15.7 DEGREES
REMARK 500    GLY B 223   CA  -  C   -  O   ANGL. DEV. = -28.1 DEGREES
REMARK 500    SER B 268   CA  -  CB  -  OG  ANGL. DEV. = -22.6 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    CYS A  24       -4.33   -169.32
REMARK 500    GLU A  27      -17.17   -140.80
REMARK 500    ALA A  30      160.16    -49.21
REMARK 500    CYS A  41       48.65   -156.02
REMARK 500    ALA A  53      152.15    179.14
REMARK 500    SER A 109      -76.62    -38.80
REMARK 500    ASN A 133       63.25   -151.13
REMARK 500    PRO A 134       -7.32    -52.03
REMARK 500    SER A 144     -149.45     67.24
REMARK 500    LEU A 164     -155.38   -127.55
REMARK 500    ASP A 165      104.24    175.12
REMARK 500    ALA A 196     -116.67     37.69
REMARK 500    ILE A 207       -9.94    -57.52
REMARK 500    ASP A 228       44.59    -85.69
REMARK 500    ALA A 241       69.36   -107.27
REMARK 500    CYS A 245     -118.40   -136.48
REMARK 500    ASP A 253       48.54    -73.93
REMARK 500    PHE A 261      -13.19     72.53
REMARK 500    GLN A 262     -174.90   -172.19
REMARK 500    THR A 292     -172.91    -61.25
REMARK 500    ARG A 317     -165.15   -100.19
REMARK 500    GLU B  27        7.35   -158.67
REMARK 500    ALA B  30      137.07    -36.82
REMARK 500    ALA B  32      177.31    -53.60
REMARK 500    ASN B  39       19.59     55.69
REMARK 500    ALA B  49       75.09    -69.25
REMARK 500    ALA B  53      146.23   -178.20
REMARK 500    ASN B  84       84.92     97.63
REMARK 500    ASP B  97     -162.72   -108.38
REMARK 500    SER B 100       45.53   -142.58
REMARK 500    ASN B 133       55.49   -161.12
REMARK 500    PRO B 134       29.83    -56.78
REMARK 500    SER B 135      -31.83   -139.86
REMARK 500    SER B 144     -141.09     37.46
REMARK 500    ALA B 187      136.99    -39.08
REMARK 500    ALA B 196     -116.83     40.16
REMARK 500    ILE B 227     -135.54   -129.98
REMARK 500    ASP B 228       18.25     33.03
REMARK 500    ILE B 231      -39.42    -26.80
REMARK 500    CYS B 245     -104.48   -123.83
REMARK 500    ASN B 246      -81.46    -69.76
REMARK 500    LEU B 258       31.85    -77.27
REMARK 500    PHE B 261      -47.88     69.95
REMARK 500    ASP B 265       33.72    -82.36
REMARK 500    LYS B 312      -18.83    -45.50
REMARK 500    ARG B 317     -118.70   -110.00
REMARK 500    CYS C  41       67.88   -168.33
REMARK 500    ALA C  49      104.16    -34.46
REMARK 500    SER C 100       46.78   -150.62
REMARK 500    SER C 144     -155.39     52.10
REMARK 500
REMARK 500 THIS ENTRY HAS      98 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS
REMARK 500
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.
REMARK 500                                 MODEL     OMEGA
REMARK 500 CYS A  267     SER A  268                   96.84
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: MAIN CHAIN PLANARITY
REMARK 500
REMARK 500 THE FOLLOWING RESIDUES HAVE A PSEUDO PLANARITY
REMARK 500 TORSION ANGLE, C(I) - CA(I) - N(I+1) - O(I), GREATER
REMARK 500 10.0 DEGREES. (M=MODEL NUMBER; RES=RESIDUE NAME;
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 500 I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        ANGLE
REMARK 500    GLY B 223         30.91
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CHIRAL CENTERS
REMARK 500
REMARK 500 UNEXPECTED CONFIGURATION OF THE FOLLOWING CHIRAL
REMARK 500 CENTER(S) USING IMPROPER CA--C--CB--N CHIRALITY
REMARK 500 FOR AMINO ACIDS AND C1'--O4'--N1(N9)--C2' FOR
REMARK 500 NUCLEIC ACIDS OR EQUIVALENT ANGLE
REMARK 500 M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,6X,F5.1,6X,A1,10X,A1,3X,A16)
REMARK 500
REMARK 500  M RES CSSEQI    IMPROPER   EXPECTED   FOUND DETAILS
REMARK 500    CYS A 267        20.7      L          L   OUTSIDE RANGE
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH C 344        DISTANCE =  6.49 ANGSTROMS
REMARK 525    HOH C 365        DISTANCE =  6.58 ANGSTROMS
DBREF  3NGM A    1   319  UNP    Q6WER3   Q6WER3_GIBZE    31    349
DBREF  3NGM B    1   319  UNP    Q6WER3   Q6WER3_GIBZE    31    349
DBREF  3NGM C    1   319  UNP    Q6WER3   Q6WER3_GIBZE    31    349
DBREF  3NGM D    1   319  UNP    Q6WER3   Q6WER3_GIBZE    31    349
SEQADV 3NGM ILE A  159  UNP  Q6WER3    VAL   189 CONFLICT
SEQADV 3NGM SER A  316  UNP  Q6WER3    ARG   346 CONFLICT
SEQADV 3NGM ARG A  317  UNP  Q6WER3    PRO   347 CONFLICT
SEQADV 3NGM SER A  318  UNP  Q6WER3    LEU   348 CONFLICT
SEQADV 3NGM SER A  319  UNP  Q6WER3    ILE   349 CONFLICT
SEQADV 3NGM ILE B  159  UNP  Q6WER3    VAL   189 CONFLICT
SEQADV 3NGM SER B  316  UNP  Q6WER3    ARG   346 CONFLICT
SEQADV 3NGM ARG B  317  UNP  Q6WER3    PRO   347 CONFLICT
SEQADV 3NGM SER B  318  UNP  Q6WER3    LEU   348 CONFLICT
SEQADV 3NGM SER B  319  UNP  Q6WER3    ILE   349 CONFLICT
SEQADV 3NGM ILE C  159  UNP  Q6WER3    VAL   189 CONFLICT
SEQADV 3NGM SER C  316  UNP  Q6WER3    ARG   346 CONFLICT
SEQADV 3NGM ARG C  317  UNP  Q6WER3    PRO   347 CONFLICT
SEQADV 3NGM SER C  318  UNP  Q6WER3    LEU   348 CONFLICT
SEQADV 3NGM SER C  319  UNP  Q6WER3    ILE   349 CONFLICT
SEQADV 3NGM ILE D  159  UNP  Q6WER3    VAL   189 CONFLICT
SEQADV 3NGM SER D  316  UNP  Q6WER3    ARG   346 CONFLICT
SEQADV 3NGM ARG D  317  UNP  Q6WER3    PRO   347 CONFLICT
SEQADV 3NGM SER D  318  UNP  Q6WER3    LEU   348 CONFLICT
SEQADV 3NGM SER D  319  UNP  Q6WER3    ILE   349 CONFLICT
SEQRES   1 A  319  ALA VAL SER VAL SER THR THR ASP PHE GLY ASN PHE LYS
SEQRES   2 A  319  PHE TYR ILE GLN HIS GLY ALA ALA ALA TYR CYS ASN SER
SEQRES   3 A  319  GLU ALA PRO ALA GLY ALA LYS VAL THR CYS SER GLY ASN
SEQRES   4 A  319  GLY CYS PRO THR VAL GLN SER ASN GLY ALA THR ILE VAL
SEQRES   5 A  319  ALA SER PHE THR GLY SER LYS THR GLY ILE GLY GLY TYR
SEQRES   6 A  319  VAL ALA THR ASP PRO THR ARG LYS GLU ILE VAL VAL SER
SEQRES   7 A  319  PHE ARG GLY SER ILE ASN ILE ARG ASN TRP LEU THR ASN
SEQRES   8 A  319  LEU ASP PHE ASP GLN ASP ASP CYS SER LEU THR SER GLY
SEQRES   9 A  319  CYS GLY VAL HIS SER GLY PHE GLN ASN ALA TRP ASN GLU
SEQRES  10 A  319  ILE SER ALA ALA ALA THR ALA ALA VAL ALA LYS ALA ARG
SEQRES  11 A  319  LYS ALA ASN PRO SER PHE LYS VAL VAL SER VAL GLY HIS
SEQRES  12 A  319  SER LEU GLY GLY ALA VAL ALA THR LEU ALA GLY ALA ASN
SEQRES  13 A  319  LEU ARG ILE GLY GLY THR PRO LEU ASP ILE TYR THR TYR
SEQRES  14 A  319  GLY SER PRO ARG VAL GLY ASN THR GLN LEU ALA ALA PHE
SEQRES  15 A  319  VAL SER ASN GLN ALA GLY GLY GLU PHE ARG VAL THR ASN
SEQRES  16 A  319  ALA LYS ASP PRO VAL PRO ARG LEU PRO PRO LEU ILE PHE
SEQRES  17 A  319  GLY TYR ARG HIS THR SER PRO GLU TYR TRP LEU SER GLY
SEQRES  18 A  319  SER GLY GLY ASP LYS ILE ASP TYR THR ILE ASN ASP VAL
SEQRES  19 A  319  LYS VAL CYS GLU GLY ALA ALA ASN LEU GLN CYS ASN GLY
SEQRES  20 A  319  GLY THR LEU GLY LEU ASP ILE ASP ALA HIS LEU HIS TYR
SEQRES  21 A  319  PHE GLN ALA THR ASP ALA CYS SER ALA GLY GLY ILE SER
SEQRES  22 A  319  TRP ARG ARG TYR ARG SER ALA LYS ARG GLU SER ILE SER
SEQRES  23 A  319  GLU ARG ALA THR MET THR ASP ALA GLU LEU GLU LYS LYS
SEQRES  24 A  319  LEU ASN SER TYR VAL GLU MET ASP LYS GLU TYR ILE LYS
SEQRES  25 A  319  THR HIS ALA SER ARG SER SER
SEQRES   1 B  319  ALA VAL SER VAL SER THR THR ASP PHE GLY ASN PHE LYS
SEQRES   2 B  319  PHE TYR ILE GLN HIS GLY ALA ALA ALA TYR CYS ASN SER
SEQRES   3 B  319  GLU ALA PRO ALA GLY ALA LYS VAL THR CYS SER GLY ASN
SEQRES   4 B  319  GLY CYS PRO THR VAL GLN SER ASN GLY ALA THR ILE VAL
SEQRES   5 B  319  ALA SER PHE THR GLY SER LYS THR GLY ILE GLY GLY TYR
SEQRES   6 B  319  VAL ALA THR ASP PRO THR ARG LYS GLU ILE VAL VAL SER
SEQRES   7 B  319  PHE ARG GLY SER ILE ASN ILE ARG ASN TRP LEU THR ASN
SEQRES   8 B  319  LEU ASP PHE ASP GLN ASP ASP CYS SER LEU THR SER GLY
SEQRES   9 B  319  CYS GLY VAL HIS SER GLY PHE GLN ASN ALA TRP ASN GLU
SEQRES  10 B  319  ILE SER ALA ALA ALA THR ALA ALA VAL ALA LYS ALA ARG
SEQRES  11 B  319  LYS ALA ASN PRO SER PHE LYS VAL VAL SER VAL GLY HIS
SEQRES  12 B  319  SER LEU GLY GLY ALA VAL ALA THR LEU ALA GLY ALA ASN
SEQRES  13 B  319  LEU ARG ILE GLY GLY THR PRO LEU ASP ILE TYR THR TYR
SEQRES  14 B  319  GLY SER PRO ARG VAL GLY ASN THR GLN LEU ALA ALA PHE
SEQRES  15 B  319  VAL SER ASN GLN ALA GLY GLY GLU PHE ARG VAL THR ASN
SEQRES  16 B  319  ALA LYS ASP PRO VAL PRO ARG LEU PRO PRO LEU ILE PHE
SEQRES  17 B  319  GLY TYR ARG HIS THR SER PRO GLU TYR TRP LEU SER GLY
SEQRES  18 B  319  SER GLY GLY ASP LYS ILE ASP TYR THR ILE ASN ASP VAL
SEQRES  19 B  319  LYS VAL CYS GLU GLY ALA ALA ASN LEU GLN CYS ASN GLY
SEQRES  20 B  319  GLY THR LEU GLY LEU ASP ILE ASP ALA HIS LEU HIS TYR
SEQRES  21 B  319  PHE GLN ALA THR ASP ALA CYS SER ALA GLY GLY ILE SER
SEQRES  22 B  319  TRP ARG ARG TYR ARG SER ALA LYS ARG GLU SER ILE SER
SEQRES  23 B  319  GLU ARG ALA THR MET THR ASP ALA GLU LEU GLU LYS LYS
SEQRES  24 B  319  LEU ASN SER TYR VAL GLU MET ASP LYS GLU TYR ILE LYS
SEQRES  25 B  319  THR HIS ALA SER ARG SER SER
SEQRES   1 C  319  ALA VAL SER VAL SER THR THR ASP PHE GLY ASN PHE LYS
SEQRES   2 C  319  PHE TYR ILE GLN HIS GLY ALA ALA ALA TYR CYS ASN SER
SEQRES   3 C  319  GLU ALA PRO ALA GLY ALA LYS VAL THR CYS SER GLY ASN
SEQRES   4 C  319  GLY CYS PRO THR VAL GLN SER ASN GLY ALA THR ILE VAL
SEQRES   5 C  319  ALA SER PHE THR GLY SER LYS THR GLY ILE GLY GLY TYR
SEQRES   6 C  319  VAL ALA THR ASP PRO THR ARG LYS GLU ILE VAL VAL SER
SEQRES   7 C  319  PHE ARG GLY SER ILE ASN ILE ARG ASN TRP LEU THR ASN
SEQRES   8 C  319  LEU ASP PHE ASP GLN ASP ASP CYS SER LEU THR SER GLY
SEQRES   9 C  319  CYS GLY VAL HIS SER GLY PHE GLN ASN ALA TRP ASN GLU
SEQRES  10 C  319  ILE SER ALA ALA ALA THR ALA ALA VAL ALA LYS ALA ARG
SEQRES  11 C  319  LYS ALA ASN PRO SER PHE LYS VAL VAL SER VAL GLY HIS
SEQRES  12 C  319  SER LEU GLY GLY ALA VAL ALA THR LEU ALA GLY ALA ASN
SEQRES  13 C  319  LEU ARG ILE GLY GLY THR PRO LEU ASP ILE TYR THR TYR
SEQRES  14 C  319  GLY SER PRO ARG VAL GLY ASN THR GLN LEU ALA ALA PHE
SEQRES  15 C  319  VAL SER ASN GLN ALA GLY GLY GLU PHE ARG VAL THR ASN
SEQRES  16 C  319  ALA LYS ASP PRO VAL PRO ARG LEU PRO PRO LEU ILE PHE
SEQRES  17 C  319  GLY TYR ARG HIS THR SER PRO GLU TYR TRP LEU SER GLY
SEQRES  18 C  319  SER GLY GLY ASP LYS ILE ASP TYR THR ILE ASN ASP VAL
SEQRES  19 C  319  LYS VAL CYS GLU GLY ALA ALA ASN LEU GLN CYS ASN GLY
SEQRES  20 C  319  GLY THR LEU GLY LEU ASP ILE ASP ALA HIS LEU HIS TYR
SEQRES  21 C  319  PHE GLN ALA THR ASP ALA CYS SER ALA GLY GLY ILE SER
SEQRES  22 C  319  TRP ARG ARG TYR ARG SER ALA LYS ARG GLU SER ILE SER
SEQRES  23 C  319  GLU ARG ALA THR MET THR ASP ALA GLU LEU GLU LYS LYS
SEQRES  24 C  319  LEU ASN SER TYR VAL GLU MET ASP LYS GLU TYR ILE LYS
SEQRES  25 C  319  THR HIS ALA SER ARG SER SER
SEQRES   1 D  319  ALA VAL SER VAL SER THR THR ASP PHE GLY ASN PHE LYS
SEQRES   2 D  319  PHE TYR ILE GLN HIS GLY ALA ALA ALA TYR CYS ASN SER
SEQRES   3 D  319  GLU ALA PRO ALA GLY ALA LYS VAL THR CYS SER GLY ASN
SEQRES   4 D  319  GLY CYS PRO THR VAL GLN SER ASN GLY ALA THR ILE VAL
SEQRES   5 D  319  ALA SER PHE THR GLY SER LYS THR GLY ILE GLY GLY TYR
SEQRES   6 D  319  VAL ALA THR ASP PRO THR ARG LYS GLU ILE VAL VAL SER
SEQRES   7 D  319  PHE ARG GLY SER ILE ASN ILE ARG ASN TRP LEU THR ASN
SEQRES   8 D  319  LEU ASP PHE ASP GLN ASP ASP CYS SER LEU THR SER GLY
SEQRES   9 D  319  CYS GLY VAL HIS SER GLY PHE GLN ASN ALA TRP ASN GLU
SEQRES  10 D  319  ILE SER ALA ALA ALA THR ALA ALA VAL ALA LYS ALA ARG
SEQRES  11 D  319  LYS ALA ASN PRO SER PHE LYS VAL VAL SER VAL GLY HIS
SEQRES  12 D  319  SER LEU GLY GLY ALA VAL ALA THR LEU ALA GLY ALA ASN
SEQRES  13 D  319  LEU ARG ILE GLY GLY THR PRO LEU ASP ILE TYR THR TYR
SEQRES  14 D  319  GLY SER PRO ARG VAL GLY ASN THR GLN LEU ALA ALA PHE
SEQRES  15 D  319  VAL SER ASN GLN ALA GLY GLY GLU PHE ARG VAL THR ASN
SEQRES  16 D  319  ALA LYS ASP PRO VAL PRO ARG LEU PRO PRO LEU ILE PHE
SEQRES  17 D  319  GLY TYR ARG HIS THR SER PRO GLU TYR TRP LEU SER GLY
SEQRES  18 D  319  SER GLY GLY ASP LYS ILE ASP TYR THR ILE ASN ASP VAL
SEQRES  19 D  319  LYS VAL CYS GLU GLY ALA ALA ASN LEU GLN CYS ASN GLY
SEQRES  20 D  319  GLY THR LEU GLY LEU ASP ILE ASP ALA HIS LEU HIS TYR
SEQRES  21 D  319  PHE GLN ALA THR ASP ALA CYS SER ALA GLY GLY ILE SER
SEQRES  22 D  319  TRP ARG ARG TYR ARG SER ALA LYS ARG GLU SER ILE SER
SEQRES  23 D  319  GLU ARG ALA THR MET THR ASP ALA GLU LEU GLU LYS LYS
SEQRES  24 D  319  LEU ASN SER TYR VAL GLU MET ASP LYS GLU TYR ILE LYS
SEQRES  25 D  319  THR HIS ALA SER ARG SER SER
FORMUL   5  HOH   *218(H2 O)
HELIX    1   1 SER A    5  SER A   26  1                                  22
HELIX    2   2 CYS A   41  ASN A   47  1                                   7
HELIX    3   3 ASN A   84  LEU A   92  1                                   9
HELIX    4   4 SER A  109  ALA A  132  1                                  24
HELIX    5   5 SER A  144  GLY A  160  1                                  17
HELIX    6   6 THR A  177  GLN A  186  1                                  10
HELIX    7   7 PRO A  199  LEU A  203  5                                   5
HELIX    8   8 PRO A  205  PHE A  208  5                                   4
HELIX    9   9 THR A  230  ASN A  232  5                                   3
HELIX   10  10 ASP A  253  LEU A  258  1                                   6
HELIX   11  11 ALA A  263  CYS A  267  5                                   5
HELIX   12  12 THR A  292  THR A  313  1                                  22
HELIX   13  13 SER B    5  ALA B   21  1                                  17
HELIX   14  14 ALA B   22  CYS B   24  5                                   3
HELIX   15  15 CYS B   41  ASN B   47  1                                   7
HELIX   16  16 ASN B   84  LEU B   92  1                                   9
HELIX   17  17 SER B  109  ALA B  132  1                                  24
HELIX   18  18 SER B  144  GLY B  161  1                                  18
HELIX   19  19 THR B  177  GLN B  186  1                                  10
HELIX   20  20 PRO B  205  PHE B  208  5                                   4
HELIX   21  21 THR B  230  ASN B  232  5                                   3
HELIX   22  22 ASP B  253  LEU B  258  1                                   6
HELIX   23  23 THR B  264  SER B  268  5                                   5
HELIX   24  24 THR B  292  ALA B  315  1                                  24
HELIX   25  25 SER C    5  ASN C   25  1                                  21
HELIX   26  26 CYS C   36  GLY C   40  5                                   5
HELIX   27  27 CYS C   41  ASN C   47  1                                   7
HELIX   28  28 ASN C   84  LEU C   92  1                                   9
HELIX   29  29 SER C  109  LYS C  131  1                                  23
HELIX   30  30 SER C  144  GLY C  161  1                                  18
HELIX   31  31 ASN C  176  GLN C  186  1                                  11
HELIX   32  32 PRO C  199  LEU C  203  5                                   5
HELIX   33  33 PRO C  205  GLY C  209  5                                   5
HELIX   34  34 CYS C  245  THR C  249  5                                   5
HELIX   35  35 ASP C  253  LEU C  258  1                                   6
HELIX   36  36 THR C  292  THR C  313  1                                  22
HELIX   37  37 SER D    5  ALA D   22  1                                  18
HELIX   38  38 TYR D   23  ALA D   28  5                                   6
HELIX   39  39 CYS D   41  SER D   46  1                                   6
HELIX   40  40 ASN D   84  LEU D   92  1                                   9
HELIX   41  41 SER D  109  ASN D  133  1                                  25
HELIX   42  42 SER D  144  THR D  162  1                                  19
HELIX   43  43 THR D  177  GLN D  186  1                                  10
HELIX   44  44 PRO D  205  PHE D  208  5                                   4
HELIX   45  45 THR D  230  VAL D  234  5                                   5
HELIX   46  46 ASP D  253  LEU D  258  1                                   6
HELIX   47  47 THR D  292  HIS D  314  1                                  23
SHEET    1   A 8 THR A  50  THR A  56  0
SHEET    2   A 8 GLY A  63  ASP A  69 -1  O  VAL A  66   N  VAL A  52
SHEET    3   A 8 GLU A  74  PHE A  79 -1  O  SER A  78   N  TYR A  65
SHEET    4   A 8 LYS A 137  HIS A 143  1  O  LYS A 137   N  ILE A  75
SHEET    5   A 8 ILE A 166  TYR A 169  1  O  TYR A 167   N  SER A 140
SHEET    6   A 8 GLU A 190  ASN A 195  1  O  VAL A 193   N  THR A 168
SHEET    7   A 8 GLU A 216  LEU A 219  1  O  LEU A 219   N  THR A 194
SHEET    8   A 8 VAL A 234  CYS A 237 -1  O  LYS A 235   N  TRP A 218
SHEET    1   B 2 GLN A  96  ASP A  98  0
SHEET    2   B 2 GLY A 106  HIS A 108 -1  O  VAL A 107   N  ASP A  97
SHEET    1   C 2 GLY A 175  ASN A 176  0
SHEET    2   C 2 TYR A 210  ARG A 211 -1  O  ARG A 211   N  GLY A 175
SHEET    1   D 8 THR B  50  THR B  56  0
SHEET    2   D 8 GLY B  63  ASP B  69 -1  O  VAL B  66   N  VAL B  52
SHEET    3   D 8 GLU B  74  PHE B  79 -1  O  VAL B  76   N  ALA B  67
SHEET    4   D 8 LYS B 137  HIS B 143  1  O  LYS B 137   N  ILE B  75
SHEET    5   D 8 LEU B 164  TYR B 169  1  O  ASP B 165   N  SER B 140
SHEET    6   D 8 GLU B 190  ASN B 195  1  O  VAL B 193   N  THR B 168
SHEET    7   D 8 GLU B 216  LEU B 219  1  O  LEU B 219   N  THR B 194
SHEET    8   D 8 VAL B 234  CYS B 237 -1  O  LYS B 235   N  TRP B 218
SHEET    1   E 2 GLN B  96  ASP B  98  0
SHEET    2   E 2 GLY B 106  HIS B 108 -1  O  VAL B 107   N  ASP B  97
SHEET    1   F 2 GLY B 175  ASN B 176  0
SHEET    2   F 2 TYR B 210  ARG B 211 -1  O  ARG B 211   N  GLY B 175
SHEET    1   G 8 THR C  50  THR C  56  0
SHEET    2   G 8 GLY C  63  ASP C  69 -1  O  VAL C  66   N  VAL C  52
SHEET    3   G 8 GLU C  74  PHE C  79 -1  O  VAL C  76   N  ALA C  67
SHEET    4   G 8 LYS C 137  HIS C 143  1  O  VAL C 139   N  ILE C  75
SHEET    5   G 8 ASP C 165  TYR C 169  1  O  TYR C 167   N  SER C 140
SHEET    6   G 8 GLU C 190  ASN C 195  1  O  VAL C 193   N  THR C 168
SHEET    7   G 8 GLU C 216  LEU C 219  1  O  LEU C 219   N  THR C 194
SHEET    8   G 8 VAL C 234  VAL C 236 -1  O  LYS C 235   N  TRP C 218
SHEET    1   H 2 GLN C  96  ASP C  97  0
SHEET    2   H 2 VAL C 107  HIS C 108 -1  O  VAL C 107   N  ASP C  97
SHEET    1   I 8 THR D  50  SER D  54  0
SHEET    2   I 8 GLY D  64  ASP D  69 -1  O  THR D  68   N  THR D  50
SHEET    3   I 8 GLU D  74  PHE D  79 -1  O  VAL D  76   N  ALA D  67
SHEET    4   I 8 LYS D 137  HIS D 143  1  O  LYS D 137   N  ILE D  75
SHEET    5   I 8 LEU D 164  TYR D 169  1  O  TYR D 169   N  GLY D 142
SHEET    6   I 8 GLU D 190  ASN D 195  1  O  PHE D 191   N  ILE D 166
SHEET    7   I 8 GLU D 216  LEU D 219  1  O  TYR D 217   N  THR D 194
SHEET    8   I 8 VAL D 236  CYS D 237 -1  O  CYS D 237   N  GLU D 216
SHEET    1   J 2 GLN D  96  ASP D  97  0
SHEET    2   J 2 VAL D 107  HIS D 108 -1  O  VAL D 107   N  ASP D  97
SHEET    1   K 2 GLY D 175  ASN D 176  0
SHEET    2   K 2 TYR D 210  ARG D 211 -1  O  ARG D 211   N  GLY D 175
SSBOND   1 CYS A   24    CYS A  267                          1555   1555  1.50
SSBOND   2 CYS A   36    CYS A   41                          1555   1555  2.04
SSBOND   3 CYS A   99    CYS A  105                          1555   1555  2.05
SSBOND   4 CYS A  237    CYS A  245                          1555   1555  2.02
SSBOND   5 CYS B   24    CYS B  267                          1555   1555  2.04
SSBOND   6 CYS B   36    CYS B   41                          1555   1555  2.04
SSBOND   7 CYS B   99    CYS B  105                          1555   1555  2.04
SSBOND   8 CYS B  237    CYS B  245                          1555   1555  2.02
SSBOND   9 CYS C   24    CYS C  267                          1555   1555  2.03
SSBOND  10 CYS C   36    CYS C   41                          1555   1555  2.04
SSBOND  11 CYS C   99    CYS C  105                          1555   1555  2.04
SSBOND  12 CYS C  237    CYS C  245                          1555   1555  2.02
SSBOND  13 CYS D   24    CYS D  267                          1555   1555  2.03
SSBOND  14 CYS D   36    CYS D   41                          1555   1555  2.03
SSBOND  15 CYS D   99    CYS D  105                          1555   1555  2.04
SSBOND  16 CYS D  237    CYS D  245                          1555   1555  2.02
CISPEP   1 LEU A  203    PRO A  204          0        -0.35
CISPEP   2 SER A  214    PRO A  215          0        -0.14
CISPEP   3 LEU B  203    PRO B  204          0         0.13
CISPEP   4 SER B  214    PRO B  215          0         0.68
CISPEP   5 LEU C  203    PRO C  204          0        -0.32
CISPEP   6 SER C  214    PRO C  215          0         0.39
CISPEP   7 LEU D  203    PRO D  204          0        -0.12
CISPEP   8 SER D  214    PRO D  215          0         0.13
CRYST1   78.417   91.001  195.802  90.00  90.00  90.00 P 21 21 21   16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.012752  0.000000  0.000000        0.00000
SCALE2      0.000000  0.010989  0.000000        0.00000
SCALE3      0.000000  0.000000  0.005107        0.00000
TER    2199      SER A 318
TER    4398      SER B 318
TER    6597      SER C 318
TER    8796      SER D 318
MASTER      532    0    0   47   46    0    0    6 9010    4   32  100
END