longtext: 3QYJ-pdb

content
HEADER    HYDROLASE                               03-MAR-11   3QYJ
TITLE     CRYSTAL STRUCTURE OF ALR0039, A PUTATIVE ALPHA/BETA HYDROLASE FROM
TITLE    2 NOSTOC SP PCC 7120.
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ALR0039 PROTEIN;
COMPND   3 CHAIN: A, B;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: NOSTOC SP.;
SOURCE   3 ORGANISM_TAXID: 103690;
SOURCE   4 STRAIN: PCC 7120 / UTEX 2576;
SOURCE   5 GENE: ALR0039;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET15B
KEYWDS    ALPHA/BETA FOLD, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    P.PETIT,O.KAGAN,A.F.YAKUNIN,SAVCHENKO A.
REVDAT   1   16-MAR-11 3QYJ    0
JRNL        AUTH   P.PETIT,O.KAGAN,A.F.YAKUNIN,SAVCHENKO A.
JRNL        TITL   CRYSTAL STRUCTURE OF ALR0039, A PUTATIVE ALPHA/BETA
JRNL        TITL 2 HYDROLASE FROM NOSTOC SP PCC 7120.
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.78 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.5.0102
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.78
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.86
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.4
REMARK   3   NUMBER OF REFLECTIONS             : 58321
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.150
REMARK   3   R VALUE            (WORKING SET) : 0.149
REMARK   3   FREE R VALUE                     : 0.179
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 3.300
REMARK   3   FREE R VALUE TEST SET COUNT      : 2021
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.78
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.82
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 4196
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 93.12
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2340
REMARK   3   BIN FREE R VALUE SET COUNT          : 0
REMARK   3   BIN FREE R VALUE                    : NULL
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 4694
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 0
REMARK   3   SOLVENT ATOMS            : 634
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 16.42
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.07000
REMARK   3    B22 (A**2) : 1.40000
REMARK   3    B33 (A**2) : -1.45000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.16000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.103
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.099
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.060
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 1.854
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.969
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.957
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  4877 ; 0.025 ; 0.021
REMARK   3   BOND LENGTHS OTHERS               (A):  3279 ; 0.001 ; 0.020
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  6643 ; 1.840 ; 1.937
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  7962 ; 1.103 ; 3.000
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   591 ; 5.985 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   241 ;33.586 ;23.320
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   790 ;13.452 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    31 ;22.956 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   713 ; 0.139 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  5444 ; 0.010 ; 0.021
REMARK   3   GENERAL PLANES OTHERS             (A):  1037 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  2923 ; 1.137 ; 1.500
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  1164 ; 0.373 ; 1.500
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  4728 ; 1.932 ; 2.000
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  1954 ; 3.271 ; 3.000
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1909 ; 4.593 ; 4.500
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.40
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING
REMARK   3  POSITIONS
REMARK   4
REMARK   4 3QYJ COMPLIES WITH FORMAT V. 3.20, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 04-MAR-11.
REMARK 100 THE RCSB ID CODE IS RCSB064264.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 08-MAY-10
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : N
REMARK 200  RADIATION SOURCE               : ROTATING ANODE
REMARK 200  BEAMLINE                       : NULL
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU FR-E+ SUPERBRIGHT
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418
REMARK 200  MONOCHROMATOR                  : GRAPHITE
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU RAXIS IV++
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-3000
REMARK 200  DATA SCALING SOFTWARE          : HKL-3000
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 60343
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.778
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.4
REMARK 200  DATA REDUNDANCY                : 3.800
REMARK 200  R MERGE                    (I) : 0.03700
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 42.8000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.78
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.81
REMARK 200  COMPLETENESS FOR SHELL     (%) : 94.5
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.60
REMARK 200  R MERGE FOR SHELL          (I) : 0.25800
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 6.300
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: PDB ENTRY 1Y37
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 49.97
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.46
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: VAPOR DIFFUSION, SITTING DROP,
REMARK 280  TEMPERATURE 298K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       44.09800
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 2150 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 20680 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -13.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000       35.53046
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000       44.09800
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000      166.93294
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     CYS B   291
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    ARG B 257   CG    ARG B 257   CD     -0.160
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    MET A 135   CG  -  SD  -  CE  ANGL. DEV. = -11.7 DEGREES
REMARK 500    ASP A 140   CB  -  CG  -  OD1 ANGL. DEV. =   6.1 DEGREES
REMARK 500    ARG B  16   NE  -  CZ  -  NH1 ANGL. DEV. =   5.6 DEGREES
REMARK 500    ARG B  16   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.8 DEGREES
REMARK 500    VAL B  71   CG1 -  CB  -  CG2 ANGL. DEV. =  12.5 DEGREES
REMARK 500    ARG B  80   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.3 DEGREES
REMARK 500    MET B 135   CG  -  SD  -  CE  ANGL. DEV. = -18.0 DEGREES
REMARK 500    ARG B 196   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.8 DEGREES
REMARK 500    ARG B 212   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES
REMARK 500    ASP B 249   CB  -  CG  -  OD1 ANGL. DEV. =   5.6 DEGREES
REMARK 500    ARG B 257   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.6 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    GLN A  36     -167.56   -117.44
REMARK 500    ASP A 104     -123.16     57.12
REMARK 500    ASN A 167       77.76   -165.05
REMARK 500    ASP A 182       89.64   -151.30
REMARK 500    THR A 216      -95.02   -130.83
REMARK 500    ASP A 225       36.01   -140.10
REMARK 500    ALA A 276       59.98   -140.21
REMARK 500    GLN B  36     -166.83   -117.68
REMARK 500    ASP B 104     -125.05     58.62
REMARK 500    ASN B 167       86.23   -161.69
REMARK 500    THR B 216      -93.89   -126.72
REMARK 500
REMARK 500 REMARK: NULL
DBREF  3QYJ A    1   291  UNP    Q8Z0Q1   Q8Z0Q1_NOSS1     1    291
DBREF  3QYJ B    1   291  UNP    Q8Z0Q1   Q8Z0Q1_NOSS1     1    291
SEQRES   1 A  291  MET PHE THR ASN PHE GLU GLN THR ILE VAL ASP THR THR
SEQRES   2 A  291  GLU ALA ARG ILE ASN LEU VAL LYS ALA GLY HIS GLY ALA
SEQRES   3 A  291  PRO LEU LEU LEU LEU HIS GLY TYR PRO GLN THR HIS VAL
SEQRES   4 A  291  MET TRP HIS LYS ILE ALA PRO LEU LEU ALA ASN ASN PHE
SEQRES   5 A  291  THR VAL VAL ALA THR ASP LEU ARG GLY TYR GLY ASP SER
SEQRES   6 A  291  SER ARG PRO ALA SER VAL PRO HIS HIS ILE ASN TYR SER
SEQRES   7 A  291  LYS ARG VAL MET ALA GLN ASP GLN VAL GLU VAL MET SER
SEQRES   8 A  291  LYS LEU GLY TYR GLU GLN PHE TYR VAL VAL GLY HIS ASP
SEQRES   9 A  291  ARG GLY ALA ARG VAL ALA HIS ARG LEU ALA LEU ASP HIS
SEQRES  10 A  291  PRO HIS ARG VAL LYS LYS LEU ALA LEU LEU ASP ILE ALA
SEQRES  11 A  291  PRO THR HIS LYS MET TYR ARG THR THR ASP GLN GLU PHE
SEQRES  12 A  291  ALA THR ALA TYR TYR HIS TRP PHE PHE LEU ILE GLN PRO
SEQRES  13 A  291  ASP ASN LEU PRO GLU THR LEU ILE GLY ALA ASN PRO GLU
SEQRES  14 A  291  TYR TYR LEU ARG LYS CYS LEU GLU LYS TRP GLY LYS ASP
SEQRES  15 A  291  PHE SER ALA PHE HIS PRO GLN ALA LEU ALA GLU TYR ILE
SEQRES  16 A  291  ARG CYS PHE SER GLN PRO ALA VAL ILE HIS ALA THR CYS
SEQRES  17 A  291  GLU ASP TYR ARG ALA ALA ALA THR ILE ASP LEU GLU HIS
SEQRES  18 A  291  ASP GLU LEU ASP MET LYS GLN LYS ILE SER CYS PRO VAL
SEQRES  19 A  291  LEU VAL LEU TRP GLY GLU LYS GLY ILE ILE GLY ARG LYS
SEQRES  20 A  291  TYR ASP VAL LEU ALA THR TRP ARG GLU ARG ALA ILE ASP
SEQRES  21 A  291  VAL SER GLY GLN SER LEU PRO CYS GLY HIS PHE LEU PRO
SEQRES  22 A  291  GLU GLU ALA PRO GLU GLU THR TYR GLN ALA ILE TYR ASN
SEQRES  23 A  291  PHE LEU THR HIS CYS
SEQRES   1 B  291  MET PHE THR ASN PHE GLU GLN THR ILE VAL ASP THR THR
SEQRES   2 B  291  GLU ALA ARG ILE ASN LEU VAL LYS ALA GLY HIS GLY ALA
SEQRES   3 B  291  PRO LEU LEU LEU LEU HIS GLY TYR PRO GLN THR HIS VAL
SEQRES   4 B  291  MET TRP HIS LYS ILE ALA PRO LEU LEU ALA ASN ASN PHE
SEQRES   5 B  291  THR VAL VAL ALA THR ASP LEU ARG GLY TYR GLY ASP SER
SEQRES   6 B  291  SER ARG PRO ALA SER VAL PRO HIS HIS ILE ASN TYR SER
SEQRES   7 B  291  LYS ARG VAL MET ALA GLN ASP GLN VAL GLU VAL MET SER
SEQRES   8 B  291  LYS LEU GLY TYR GLU GLN PHE TYR VAL VAL GLY HIS ASP
SEQRES   9 B  291  ARG GLY ALA ARG VAL ALA HIS ARG LEU ALA LEU ASP HIS
SEQRES  10 B  291  PRO HIS ARG VAL LYS LYS LEU ALA LEU LEU ASP ILE ALA
SEQRES  11 B  291  PRO THR HIS LYS MET TYR ARG THR THR ASP GLN GLU PHE
SEQRES  12 B  291  ALA THR ALA TYR TYR HIS TRP PHE PHE LEU ILE GLN PRO
SEQRES  13 B  291  ASP ASN LEU PRO GLU THR LEU ILE GLY ALA ASN PRO GLU
SEQRES  14 B  291  TYR TYR LEU ARG LYS CYS LEU GLU LYS TRP GLY LYS ASP
SEQRES  15 B  291  PHE SER ALA PHE HIS PRO GLN ALA LEU ALA GLU TYR ILE
SEQRES  16 B  291  ARG CYS PHE SER GLN PRO ALA VAL ILE HIS ALA THR CYS
SEQRES  17 B  291  GLU ASP TYR ARG ALA ALA ALA THR ILE ASP LEU GLU HIS
SEQRES  18 B  291  ASP GLU LEU ASP MET LYS GLN LYS ILE SER CYS PRO VAL
SEQRES  19 B  291  LEU VAL LEU TRP GLY GLU LYS GLY ILE ILE GLY ARG LYS
SEQRES  20 B  291  TYR ASP VAL LEU ALA THR TRP ARG GLU ARG ALA ILE ASP
SEQRES  21 B  291  VAL SER GLY GLN SER LEU PRO CYS GLY HIS PHE LEU PRO
SEQRES  22 B  291  GLU GLU ALA PRO GLU GLU THR TYR GLN ALA ILE TYR ASN
SEQRES  23 B  291  PHE LEU THR HIS CYS
FORMUL   3  HOH   *634(H2 O)
HELIX    1   1 THR A   37  HIS A   42  5                                   6
HELIX    2   2 ILE A   44  ALA A   49  1                                   6
HELIX    3   3 VAL A   71  TYR A   77  5                                   7
HELIX    4   4 SER A   78  LEU A   93  1                                  16
HELIX    5   5 ASP A  104  HIS A  117  1                                  14
HELIX    6   6 PRO A  131  THR A  138  1                                   8
HELIX    7   7 ASP A  140  TYR A  147  1                                   8
HELIX    8   8 TYR A  148  LEU A  153  1                                   6
HELIX    9   9 ASN A  158  ALA A  166  1                                   9
HELIX   10  10 ASN A  167  GLY A  180  1                                  14
HELIX   11  11 ASP A  182  PHE A  186  5                                   5
HELIX   12  12 HIS A  187  SER A  199  1                                  13
HELIX   13  13 GLN A  200  ALA A  215  1                                  16
HELIX   14  14 THR A  216  LEU A  224  1                                   9
HELIX   15  15 ILE A  243  TYR A  248  1                                   6
HELIX   16  16 ASP A  249  GLU A  256  1                                   8
HELIX   17  17 PHE A  271  ALA A  276  1                                   6
HELIX   18  18 ALA A  276  HIS A  290  1                                  15
HELIX   19  19 THR B   37  HIS B   42  5                                   6
HELIX   20  20 ILE B   44  ALA B   49  1                                   6
HELIX   21  21 VAL B   71  TYR B   77  5                                   7
HELIX   22  22 SER B   78  LEU B   93  1                                  16
HELIX   23  23 ASP B  104  HIS B  117  1                                  14
HELIX   24  24 PRO B  131  THR B  138  1                                   8
HELIX   25  25 ASP B  140  TYR B  147  1                                   8
HELIX   26  26 TYR B  148  LEU B  153  1                                   6
HELIX   27  27 ASN B  158  ALA B  166  1                                   9
HELIX   28  28 ASN B  167  GLY B  180  1                                  14
HELIX   29  29 ASP B  182  PHE B  186  5                                   5
HELIX   30  30 HIS B  187  SER B  199  1                                  13
HELIX   31  31 GLN B  200  ALA B  215  1                                  16
HELIX   32  32 THR B  216  ASP B  225  1                                  10
HELIX   33  33 ILE B  243  TYR B  248  1                                   6
HELIX   34  34 ASP B  249  GLU B  256  1                                   8
HELIX   35  35 PHE B  271  ALA B  276  1                                   6
HELIX   36  36 ALA B  276  HIS B  290  1                                  15
SHEET    1   A 8 GLU A   6  ASP A  11  0
SHEET    2   A 8 ARG A  16  ALA A  22 -1  O  ILE A  17   N  VAL A  10
SHEET    3   A 8 THR A  53  THR A  57 -1  O  VAL A  54   N  ALA A  22
SHEET    4   A 8 PRO A  27  LEU A  31  1  N  LEU A  28   O  VAL A  55
SHEET    5   A 8 PHE A  98  HIS A 103  1  O  TYR A  99   N  LEU A  29
SHEET    6   A 8 VAL A 121  LEU A 127  1  O  LEU A 127   N  GLY A 102
SHEET    7   A 8 VAL A 234  GLY A 239  1  O  LEU A 235   N  LEU A 124
SHEET    8   A 8 VAL A 261  LEU A 266  1  O  LEU A 266   N  TRP A 238
SHEET    1   B 8 GLU B   6  ASP B  11  0
SHEET    2   B 8 ARG B  16  ALA B  22 -1  O  ILE B  17   N  VAL B  10
SHEET    3   B 8 PHE B  52  THR B  57 -1  O  VAL B  54   N  ALA B  22
SHEET    4   B 8 ALA B  26  LEU B  31  1  N  LEU B  28   O  VAL B  55
SHEET    5   B 8 PHE B  98  HIS B 103  1  O  TYR B  99   N  LEU B  29
SHEET    6   B 8 VAL B 121  LEU B 127  1  O  ALA B 125   N  VAL B 100
SHEET    7   B 8 VAL B 234  GLY B 239  1  O  LEU B 235   N  LEU B 126
SHEET    8   B 8 VAL B 261  LEU B 266  1  O  LEU B 266   N  TRP B 238
CISPEP   1 TYR A   34    PRO A   35          0        -8.13
CISPEP   2 TYR B   34    PRO B   35          0        -5.95
CRYST1   44.542   88.196   83.588  90.00  93.09  90.00 P 1 21 1      4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.022451  0.000000  0.001213        0.00000
SCALE2      0.000000  0.011338  0.000000        0.00000
SCALE3      0.000000  0.000000  0.011981        0.00000
TER    2378      CYS A 291
TER    4734      HIS B 290
MASTER      326    0    0   36   16    0    0    6 5328    2    0   46
END