longtext: 3V9A-pdb

content
HEADER    HYDROLASE ACTIVATOR                     24-DEC-11   3V9A
TITLE     CRYSTAL STRUCTURE OF ESTERASE/LIPASE FROM UNCULTURED BACTERIUM
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ESTERASE/LIPASE;
COMPND   3 CHAIN: A;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: UNCULTURED BACTERIUM;
SOURCE   3 ORGANISM_TAXID: 77133;
SOURCE   4 GENE: ESTE5;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    ESTERASE, LIPASE, HYDROLASE ACTIVATOR
EXPDTA    X-RAY DIFFRACTION
AUTHOR    I.J.KIM,K.H.NAM
REVDAT   1   15-FEB-12 3V9A    0
JRNL        AUTH   I.J.KIM,K.H.NAM
JRNL        TITL   CRYSTAL STRUCTURE OF ESTERASE/LIPASE FROM UNCULTURED
JRNL        TITL 2 BACTERIUM
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.07 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.7.2_869)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER
REMARK   3               : ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.07
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 47.24
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.500
REMARK   3   COMPLETENESS FOR RANGE        (%) : 93.0
REMARK   3   NUMBER OF REFLECTIONS             : 16708
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.192
REMARK   3   R VALUE            (WORKING SET) : 0.187
REMARK   3   FREE R VALUE                     : 0.239
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 10.000
REMARK   3   FREE R VALUE TEST SET COUNT      : 1671
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 47.2522 -  4.7387    0.93     1392   155  0.1793 0.2009
REMARK   3     2  4.7387 -  3.7617    0.95     1315   146  0.1554 0.2138
REMARK   3     3  3.7617 -  3.2864    0.95     1295   144  0.1778 0.2389
REMARK   3     4  3.2864 -  2.9859    0.95     1276   142  0.1924 0.2487
REMARK   3     5  2.9859 -  2.7719    0.95     1277   142  0.1943 0.2732
REMARK   3     6  2.7719 -  2.6085    0.94     1264   140  0.1917 0.2465
REMARK   3     7  2.6085 -  2.4779    0.94     1242   138  0.1806 0.2412
REMARK   3     8  2.4779 -  2.3700    0.94     1240   138  0.1850 0.2318
REMARK   3     9  2.3700 -  2.2788    0.93     1225   135  0.2101 0.2620
REMARK   3    10  2.2788 -  2.2002    0.86     1127   126  0.2653 0.3628
REMARK   3    11  2.2002 -  2.1314    0.94     1236   138  0.2275 0.2750
REMARK   3    12  2.1314 -  2.0705    0.88     1148   127  0.2575 0.2793
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.20
REMARK   3   SHRINKAGE RADIUS   : 0.98
REMARK   3   K_SOL              : 0.37
REMARK   3   B_SOL              : 39.41
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.480
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 20.260
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 37.10
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 2.43130
REMARK   3    B22 (A**2) : 2.43130
REMARK   3    B33 (A**2) : -4.86260
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : -0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.004           2278
REMARK   3   ANGLE     :  0.916           3092
REMARK   3   CHIRALITY :  0.057            337
REMARK   3   PLANARITY :  0.004            401
REMARK   3   DIHEDRAL  : 15.745            833
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 1
REMARK   3   TLS GROUP : 1
REMARK   3    SELECTION: all
REMARK   3    ORIGIN FOR THE GROUP (A): -12.2949  15.2404  -0.0296
REMARK   3    T TENSOR
REMARK   3      T11:   0.1424 T22:   0.1418
REMARK   3      T33:   0.1674 T12:  -0.0165
REMARK   3      T13:  -0.0063 T23:  -0.0105
REMARK   3    L TENSOR
REMARK   3      L11:   2.1035 L22:   2.1435
REMARK   3      L33:   2.4401 L12:   0.2233
REMARK   3      L13:   0.3856 L23:   0.2373
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0410 S12:   0.1741 S13:  -0.1020
REMARK   3      S21:  -0.0877 S22:   0.1054 S23:   0.0098
REMARK   3      S31:   0.0792 S32:   0.0902 S33:  -0.0395
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 3V9A COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 06-JAN-11.
REMARK 100 THE RCSB ID CODE IS RCSB069756.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 20-FEB-09
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 7.0
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : PAL/PLS
REMARK 200  BEAMLINE                       : 4A
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.000
REMARK 200  MONOCHROMATOR                  : SI
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 210
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL
REMARK 200  DATA SCALING SOFTWARE          : HKL
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 16720
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.070
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 1.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 92.7
REMARK 200  DATA REDUNDANCY                : NULL
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : NULL
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.07
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.11
REMARK 200  COMPLETENESS FOR SHELL     (%) : 84.0
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHENIX.AUTOMR
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 40.97
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.08
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M TRIS, PH 7.0, 2.0M AMMONIUM
REMARK 280  SULFATE, 0.2M AMMONIUM SULFATE, VAPOR DIFFUSION, HANGING DROP,
REMARK 280  TEMPERATURE 295K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 41 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z+1/2
REMARK 290       3555   -Y+1/2,X+1/2,Z+1/4
REMARK 290       4555   Y+1/2,-X+1/2,Z+3/4
REMARK 290       5555   -X+1/2,Y+1/2,-Z+1/4
REMARK 290       6555   X+1/2,-Y+1/2,-Z+3/4
REMARK 290       7555   Y,X,-Z
REMARK 290       8555   -Y,-X,-Z+1/2
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       74.55850
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       30.53000
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       30.53000
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       37.27925
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       30.53000
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       30.53000
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000      111.83775
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       30.53000
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       30.53000
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       37.27925
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       30.53000
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       30.53000
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000      111.83775
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       74.55850
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     VAL A   194
REMARK 465     ALA A   195
REMARK 465     PRO A   196
REMARK 465     LEU A   298
REMARK 465     ALA A   299
REMARK 465     ALA A   300
REMARK 465     ALA A   301
REMARK 465     LEU A   302
REMARK 465     GLU A   303
REMARK 465     HIS A   304
REMARK 465     HIS A   305
REMARK 465     HIS A   306
REMARK 465     HIS A   307
REMARK 465     HIS A   308
REMARK 465     HIS A   309
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    HOH A   558     O    HOH A   561              1.99
REMARK 500   NH2  ARG A   126     O    HOH A   600              2.08
REMARK 500   O    HOH A   571     O    HOH A   603              2.09
REMARK 500   O    HOH A   577     O    HOH A   585              2.10
REMARK 500   O    HOH A   601     O    HOH A   602              2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A 144     -122.98     57.77
REMARK 500    ASP A 237       56.16   -100.61
REMARK 500    ASP A 265       -0.33     67.25
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE SO4 A 401
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE SO4 A 402
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE SO4 A 403
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 3FAK   RELATED DB: PDB
DBREF  3V9A A    1   297  UNP    Q0GMU2   Q0GMU2_9BACT     1    297
SEQADV 3V9A LEU A  298  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A ALA A  299  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A ALA A  300  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A ALA A  301  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A LEU A  302  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A GLU A  303  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A HIS A  304  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A HIS A  305  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A HIS A  306  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A HIS A  307  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A HIS A  308  UNP  Q0GMU2              EXPRESSION TAG
SEQADV 3V9A HIS A  309  UNP  Q0GMU2              EXPRESSION TAG
SEQRES   1 A  309  MET ALA GLY PRO GLU ILE VAL LYS LEU LYS LYS ILE LEU
SEQRES   2 A  309  ARG GLU LYS ALA VAL PRO PRO GLY THR GLU VAL PRO LEU
SEQRES   3 A  309  ASP VAL MET ARG LYS GLY MET GLU LYS VAL ALA PHE LYS
SEQRES   4 A  309  ALA ALA ASP ASP ILE GLN VAL GLU GLN VAL THR VAL ALA
SEQRES   5 A  309  GLY CYS ALA ALA GLU TRP VAL ARG ALA PRO GLY CYS GLN
SEQRES   6 A  309  ALA GLY LYS ALA ILE LEU TYR LEU HIS GLY GLY GLY TYR
SEQRES   7 A  309  VAL MET GLY SER ILE ASN THR HIS ARG SER MET VAL GLY
SEQRES   8 A  309  GLU ILE SER ARG ALA SER GLN ALA ALA ALA LEU LEU LEU
SEQRES   9 A  309  ASP TYR ARG LEU ALA PRO GLU HIS PRO PHE PRO ALA ALA
SEQRES  10 A  309  VAL GLU ASP GLY VAL ALA ALA TYR ARG TRP LEU LEU ASP
SEQRES  11 A  309  GLN GLY PHE LYS PRO GLN HIS LEU SER ILE SER GLY ASP
SEQRES  12 A  309  SER ALA GLY GLY GLY LEU VAL LEU ALA VAL LEU VAL SER
SEQRES  13 A  309  ALA ARG ASP GLN GLY LEU PRO MET PRO ALA SER ALA ILE
SEQRES  14 A  309  PRO ILE SER PRO TRP ALA ASP MET THR CYS THR ASN ASP
SEQRES  15 A  309  SER PHE LYS THR ARG ALA GLU ALA ASP PRO MET VAL ALA
SEQRES  16 A  309  PRO GLY GLY ILE ASN LYS MET ALA ALA ARG TYR LEU ASN
SEQRES  17 A  309  GLY ALA ASP ALA LYS HIS PRO TYR ALA SER PRO ASN PHE
SEQRES  18 A  309  ALA ASN LEU LYS GLY LEU PRO PRO LEU LEU ILE HIS VAL
SEQRES  19 A  309  GLY ARG ASP GLU VAL LEU LEU ASP ASP SER ILE LYS LEU
SEQRES  20 A  309  ASP ALA LYS ALA LYS ALA ASP GLY VAL LYS SER THR LEU
SEQRES  21 A  309  GLU ILE TRP ASP ASP MET ILE HIS VAL TRP HIS ALA PHE
SEQRES  22 A  309  HIS PRO MET LEU PRO GLU GLY LYS GLN ALA ILE VAL ARG
SEQRES  23 A  309  VAL GLY GLU PHE MET ARG GLU GLN TRP ALA ALA LEU ALA
SEQRES  24 A  309  ALA ALA LEU GLU HIS HIS HIS HIS HIS HIS
HET    SO4  A 401       5
HET    SO4  A 402       5
HET    SO4  A 403       5
HETNAM     SO4 SULFATE ION
FORMUL   2  SO4    3(O4 S 2-)
FORMUL   5  HOH   *106(H2 O)
HELIX    1   1 GLY A    3  ALA A   17  1                                  15
HELIX    2   2 PRO A   25  VAL A   36  1                                  12
HELIX    3   3 SER A   82  GLN A   98  1                                  17
HELIX    4   4 PRO A  115  GLY A  132  1                                  18
HELIX    5   5 LYS A  134  GLN A  136  5                                   3
HELIX    6   6 SER A  144  GLN A  160  1                                  17
HELIX    7   7 ASP A  182  ARG A  187  1                                   6
HELIX    8   8 GLY A  198  ASN A  208  1                                  11
HELIX    9   9 SER A  218  ALA A  222  5                                   5
HELIX   10  10 LEU A  240  ASP A  254  1                                  15
HELIX   11  11 VAL A  269  HIS A  274  5                                   6
HELIX   12  12 LEU A  277  ALA A  296  1                                  20
SHEET    1   A 6 GLN A  45  VAL A  51  0
SHEET    2   A 6 CYS A  54  ARG A  60 -1  O  CYS A  54   N  VAL A  51
SHEET    3   A 6 ALA A 100  LEU A 104 -1  O  ALA A 101   N  VAL A  59
SHEET    4   A 6 ALA A  69  LEU A  73  1  N  ILE A  70   O  LEU A 102
SHEET    5   A 6 LEU A 138  ASP A 143  1  O  SER A 141   N  LEU A  73
SHEET    6   A 6 SER A 167  ILE A 171  1  O  ILE A 171   N  GLY A 142
SHEET    1   B 2 LEU A 230  GLY A 235  0
SHEET    2   B 2 SER A 258  TRP A 263  1  O  THR A 259   N  ILE A 232
CISPEP   1 ALA A  109    PRO A  110          0         0.68
CISPEP   2 PHE A  114    PRO A  115          0         7.25
SITE     1 AC1  6 ASP A 105  TYR A 106  ARG A 107  LEU A 108
SITE     2 AC1  6 HIS A 112  HOH A 521
SITE     1 AC2  4 LYS A  10  ARG A  14  LYS A  39  ALA A 190
SITE     1 AC3  4 PRO A  25  LEU A  26  ARG A 205  HOH A 605
CRYST1   61.060   61.060  149.117  90.00  90.00  90.00 P 41 21 2     8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.016377  0.000000  0.000000        0.00000
SCALE2      0.000000  0.016377  0.000000        0.00000
SCALE3      0.000000  0.000000  0.006706        0.00000
TER    2215      ALA A 297
MASTER      306    0    3   12    8    0    4    6 2335    1   15   24
END