longtext: 4BE9-pdb

content
HEADER    HYDROLASE                               06-MAR-13   4BE9
TITLE     OPEN CONFORMATION OF O. PICEAE STEROL ESTERASE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: STEROL ESTERASE;
COMPND   3 CHAIN: A, B;
COMPND   4 FRAGMENT: RESIDUES 13-549;
COMPND   5 EC: 3.1.1.13;
COMPND   6 ENGINEERED: YES;
COMPND   7 OTHER_DETAILS: N-ACETYLGLUCOSAMINE GLYCOSYLATIONS IN POSITIONS 362
COMPND   8  AND 380
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: OPHIOSTOMA PICEAE;
SOURCE   3 ORGANISM_TAXID: 61273;
SOURCE   4 EXPRESSION_SYSTEM: KOMAGATAELLA PASTORIS;
SOURCE   5 EXPRESSION_SYSTEM_TAXID: 4922;
SOURCE   6 EXPRESSION_SYSTEM_STRAIN: KM71;
SOURCE   7 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   8 EXPRESSION_SYSTEM_VECTOR: PPIC9
KEYWDS    HYDROLASE, OPHIOSTOMA
EXPDTA    X-RAY DIFFRACTION
AUTHOR    J.GUTIERREZ-FERNANDEZ,M.E.VAQUERO,A.PRIETO,J.BARRIUSO,M.J.GONZALEZ,
AUTHOR   2 J.A.HERMOSO
REVDAT   1   26-MAR-14 4BE9    0
JRNL        AUTH   J.GUTIERREZ-FERNANDEZ,M.E.VAQUERO,A.PRIETO,J.BARRIUSO,
JRNL        AUTH 2 M.J.GONZALEZ,J.A.HERMOSO
JRNL        TITL   CRYSTAL STRUCTURE OF OPE
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.00 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.000
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 43.561
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.34
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.99
REMARK   3   NUMBER OF REFLECTIONS             : 84172
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.1694
REMARK   3   R VALUE            (WORKING SET) : 0.1675
REMARK   3   FREE R VALUE                     : 0.2059
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.0
REMARK   3   FREE R VALUE TEST SET COUNT      : 4204
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 43.5710 -  6.2088    1.00     2739   152  0.1953 0.1948
REMARK   3     2  6.2088 -  4.9303    1.00     2699   141  0.1816 0.1989
REMARK   3     3  4.9303 -  4.3077    1.00     2675   154  0.1365 0.1777
REMARK   3     4  4.3077 -  3.9141    1.00     2681   144  0.1487 0.1774
REMARK   3     5  3.9141 -  3.6337    1.00     2687   146  0.1556 0.1969
REMARK   3     6  3.6337 -  3.4196    1.00     2656   149  0.1595 0.1880
REMARK   3     7  3.4196 -  3.2484    1.00     2675   131  0.1589 0.2005
REMARK   3     8  3.2484 -  3.1070    1.00     2663   149  0.1548 0.1742
REMARK   3     9  3.1070 -  2.9874    1.00     2664   129  0.1591 0.1970
REMARK   3    10  2.9874 -  2.8844    1.00     2690   134  0.1499 0.1877
REMARK   3    11  2.8844 -  2.7942    1.00     2672   138  0.1516 0.2022
REMARK   3    12  2.7942 -  2.7143    1.00     2636   163  0.1625 0.1967
REMARK   3    13  2.7143 -  2.6429    1.00     2665   138  0.1572 0.2291
REMARK   3    14  2.6429 -  2.5784    1.00     2661   144  0.1602 0.2107
REMARK   3    15  2.5784 -  2.5198    1.00     2630   143  0.1649 0.2329
REMARK   3    16  2.5198 -  2.4662    1.00     2636   148  0.1689 0.2263
REMARK   3    17  2.4662 -  2.4169    1.00     2682   134  0.1709 0.2238
REMARK   3    18  2.4169 -  2.3713    1.00     2657   139  0.1652 0.2435
REMARK   3    19  2.3713 -  2.3289    1.00     2686   127  0.1666 0.1848
REMARK   3    20  2.3289 -  2.2894    1.00     2647   119  0.1708 0.2091
REMARK   3    21  2.2894 -  2.2525    1.00     2617   153  0.1822 0.2702
REMARK   3    22  2.2525 -  2.2178    1.00     2674   143  0.1773 0.2592
REMARK   3    23  2.2178 -  2.1852    1.00     2663   150  0.1815 0.2520
REMARK   3    24  2.1852 -  2.1545    1.00     2669   130  0.1888 0.2462
REMARK   3    25  2.1545 -  2.1253    1.00     2628   131  0.1936 0.2360
REMARK   3    26  2.1253 -  2.0977    1.00     2690   144  0.1953 0.2495
REMARK   3    27  2.0977 -  2.0715    1.00     2655   131  0.2082 0.2627
REMARK   3    28  2.0715 -  2.0466    1.00     2656   129  0.2073 0.2775
REMARK   3    29  2.0466 -  2.0228    1.00     2653   144  0.2378 0.2755
REMARK   3    30  2.0228 -  2.0000    1.00     2662   127  0.2697 0.2975
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.20
REMARK   3   SHRINKAGE RADIUS   : 0.98
REMARK   3   K_SOL              : 0.328
REMARK   3   B_SOL              : 41.224
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.53
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 20.19
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 27.80
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 1.6444
REMARK   3    B22 (A**2) : 1.6444
REMARK   3    B33 (A**2) : -3.2889
REMARK   3    B12 (A**2) : 0.0000
REMARK   3    B13 (A**2) : 0.0000
REMARK   3    B23 (A**2) : 0.0000
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.008           8743
REMARK   3   ANGLE     :  1.051          11877
REMARK   3   CHIRALITY :  0.073           1323
REMARK   3   PLANARITY :  0.005           1527
REMARK   3   DIHEDRAL  : 14.118           3136
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 1
REMARK   3   TLS GROUP : 1
REMARK   3    SELECTION: ALL
REMARK   3    ORIGIN FOR THE GROUP (A):  31.4231   0.2326  28.1470
REMARK   3    T TENSOR
REMARK   3      T11:   0.0070 T22:   0.0676
REMARK   3      T33:   0.0352 T12:   0.0846
REMARK   3      T13:   0.0328 T23:  -0.0178
REMARK   3    L TENSOR
REMARK   3      L11:   0.4222 L22:   0.4489
REMARK   3      L33:   0.5376 L12:  -0.0740
REMARK   3      L13:  -0.0914 L23:   0.1270
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0015 S12:  -0.2018 S13:  -0.0904
REMARK   3      S21:   0.2746 S22:   0.0089 S23:  -0.0598
REMARK   3      S31:   0.0889 S32:   0.0832 S33:  -0.0418
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: THE FIRST 8 AMINO ACIDS OF THE SAMPLE
REMARK   3 (EAEAYVEF) CORRESPOND TO A PURIFICATION TAG. THIS REGION IS MAINLY
REMARK   3 DISORDERED IN THE CRYSTAL.
REMARK   4
REMARK   4 4BE9 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 07-MAR-13.
REMARK 100 THE PDBE ID CODE IS EBI-55740.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : NULL
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 7.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : ESRF
REMARK 200  BEAMLINE                       : ID29
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97908
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL (PILATUS 6M)
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 84190
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.00
REMARK 200  RESOLUTION RANGE LOW       (A) : 47.28
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 6.0
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0
REMARK 200  DATA REDUNDANCY                : 7.0
REMARK 200  R MERGE                    (I) : 0.11
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 11.70
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.11
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.1
REMARK 200  R MERGE FOR SHELL          (I) : 0.81
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 2.50
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: PDB ENTRY 1LPN
REMARK 200
REMARK 200 REMARK: NONE
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 53.6
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.65
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M SODIUM NITRATE, 0.1 M
REMARK 280  BIS-TRIS PROPANE PH 7.5, 20% PEG 3350
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: I 41
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y+1/2,Z+1/2
REMARK 290       3555   -Y,X+1/2,Z+1/4
REMARK 290       4555   Y+1/2,-X,Z+3/4
REMARK 290       5555   X+1/2,Y+1/2,Z+1/2
REMARK 290       6555   -X,-Y,Z
REMARK 290       7555   -Y+1/2,X,Z+3/4
REMARK 290       8555   Y,-X+1/2,Z+1/4
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       82.06150
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000       82.06150
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       46.99700
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       82.06150
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       23.49850
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       82.06150
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       70.49550
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       82.06150
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       82.06150
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       46.99700
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   7  0.000000 -1.000000  0.000000       82.06150
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  0.000000  1.000000       70.49550
REMARK 290   SMTRY1   8  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000       82.06150
REMARK 290   SMTRY3   8  0.000000  0.000000  1.000000       23.49850
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 7760 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 35420 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -3.5 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     GLU A     5
REMARK 465     ALA A     6
REMARK 465     GLU A     7
REMARK 465     ALA A     8
REMARK 465     TYR A     9
REMARK 465     VAL A    10
REMARK 465     GLU A    11
REMARK 465     GLU B     5
REMARK 465     ALA B     6
REMARK 465     GLU B     7
REMARK 465     ALA B     8
REMARK 465     TYR B     9
REMARK 465     VAL B    10
REMARK 465     GLU B    11
REMARK 465     PHE B    12
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OD2  ASP A   293     O    HOH A  2340              2.12
REMARK 500   OD1  ASN A   391     O    HOH A  2393              1.91
REMARK 500   OD2  ASP A   396     OG1  THR A   398              2.20
REMARK 500   NE2  GLN A   510     O    HOH A  2495              2.08
REMARK 500   OH   TYR A   541     O    HOH A  2451              2.13
REMARK 500   ND2  ASN B    18     OE1  GLU B    23              2.04
REMARK 500   NH2  ARG B    55     O    HOH B  2004              2.02
REMARK 500   OG   SER B   123     O    HOH B  2059              2.16
REMARK 500   ND2  ASN B   203     O    HOH B  2004              2.19
REMARK 500   N    LEU B   477     O8   7P9 B  1551              2.11
REMARK 500   O7   NAG A  1550     O    HOH A  2395              2.12
REMARK 500   O2   7P9 A  1552     O    HOH A  2472              2.09
REMARK 500   O2   NO3 A  1557     O    HOH A  2531              2.09
REMARK 500   O    HOH A  2065     O    HOH A  2339              2.16
REMARK 500   O    HOH A  2123     O    HOH A  2278              2.08
REMARK 500   O    HOH A  2140     O    HOH A  2295              2.17
REMARK 500   O    HOH A  2461     O    HOH A  2462              1.97
REMARK 500   O    HOH B  2017     O    HOH B  2049              2.05
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PHE A 136       19.78     59.51
REMARK 500    SER A 220     -120.16     63.87
REMARK 500    SER A 252       76.63     68.91
REMARK 500    SER A 254      -90.92   -153.91
REMARK 500    SER A 309     -178.74   -177.27
REMARK 500    SER A 312     -121.71     47.66
REMARK 500    VAL A 324      -59.18   -123.73
REMARK 500    ASP A 351       63.49   -115.30
REMARK 500    TYR A 377      -70.92   -102.67
REMARK 500    ASN A 409       11.91     81.65
REMARK 500    PHE A 427      -70.08   -126.30
REMARK 500    PHE A 460      -36.93     69.16
REMARK 500    ASN A 479     -168.90   -165.63
REMARK 500    VAL B  15      117.21   -167.81
REMARK 500    LEU B  30       65.59     -7.70
REMARK 500    PHE B 136       17.83     58.79
REMARK 500    SER B 220     -118.81     50.25
REMARK 500    SER B 252       77.84     70.96
REMARK 500    SER B 254      -97.62   -152.81
REMARK 500    SER B 312     -122.09     46.33
REMARK 500    VAL B 324      -54.64   -133.02
REMARK 500    PRO B 343      141.75    -39.65
REMARK 500    ASP B 351       61.10   -115.22
REMARK 500    PRO B 360       47.63    -72.70
REMARK 500    TYR B 377      -65.69   -100.40
REMARK 500    ASN B 380       40.59   -104.86
REMARK 500    ASN B 409       13.48     80.02
REMARK 500    PHE B 427      -65.19   -127.11
REMARK 500    PHE B 460      -43.73     73.00
REMARK 500    ALA B 466        2.07     81.67
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CHIRAL CENTERS
REMARK 500
REMARK 500 UNEXPECTED CONFIGURATION OF THE FOLLOWING CHIRAL
REMARK 500 CENTER(S) USING IMPROPER CA--C--CB--N CHIRALITY
REMARK 500 FOR AMINO ACIDS AND C1'--O4'--N1(N9)--C2' FOR
REMARK 500 NUCLEIC ACIDS OR EQUIVALENT ANGLE
REMARK 500 M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,6X,F5.1,6X,A1,10X,A1,3X,A16)
REMARK 500
REMARK 500  M RES CSSEQI    IMPROPER   EXPECTED   FOUND DETAILS
REMARK 500    ASN B  16        24.2      L          L   OUTSIDE RANGE
REMARK 500
REMARK 500 REMARK: NULL
REMARK 600
REMARK 600 HETEROGEN
REMARK 600 N-ACETYL-D-GLUCOSAMINE (NAG): COVALENTLY BOUND TO A-ASN362,
REMARK 600  A-ASN380 AND B-ASN362 RESPECTIVELY, BY N-GLYCOSIDIC
REMARK 600  LINKAGES
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE 7P9 A1552
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE 7P9 B1551
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE 1PE A1553
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PGE A1554
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG A1555
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B1552
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B1553
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B1554
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B1555
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PGE B1556
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A1556
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B1557
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B1558
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NO3 A1557
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NO3 B1559
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR MONO-SACCHARIDE
REMARK 800  NAG A1550  BOUND TO ASN A 362
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR MONO-SACCHARIDE
REMARK 800  NAG A1551  BOUND TO ASN A 380
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR MONO-SACCHARIDE
REMARK 800  NAG B1550  BOUND TO ASN B 362
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4BE4   RELATED DB: PDB
REMARK 900  CLOSED CONFORMATION OF O. PICEAE STEROL ESTERASE
DBREF  4BE9 A   13   549  UNP    Q2TFW1   Q2TFW1_9PEZI    13    549
DBREF  4BE9 B   13   549  UNP    Q2TFW1   Q2TFW1_9PEZI    13    549
SEQADV 4BE9 GLU A    5  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 ALA A    6  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 GLU A    7  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 ALA A    8  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 TYR A    9  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 VAL A   10  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 GLU A   11  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 PHE A   12  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 GLU B    5  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 ALA B    6  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 GLU B    7  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 ALA B    8  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 TYR B    9  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 VAL B   10  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 GLU B   11  UNP  Q2TFW1              EXPRESSION TAG
SEQADV 4BE9 PHE B   12  UNP  Q2TFW1              EXPRESSION TAG
SEQRES   1 A  545  GLU ALA GLU ALA TYR VAL GLU PHE THR THR VAL ASN VAL
SEQRES   2 A  545  ASN TYR PRO GLU GLY GLU VAL VAL GLY VAL SER VAL LEU
SEQRES   3 A  545  GLY ILE GLU SER PHE ARG GLY VAL PRO PHE ALA GLN PRO
SEQRES   4 A  545  PRO VAL GLY ASN LEU ARG LEU LYS PRO PRO VAL ARG TYR
SEQRES   5 A  545  THR GLU ASN ILE GLY THR LYS ASP THR THR GLY ILE GLY
SEQRES   6 A  545  PRO SER CYS PRO GLN MET TYR LEU SER THR GLY ASN GLY
SEQRES   7 A  545  GLU LEU LEU PHE GLN LEU VAL GLY ASN LEU ILE ASN ILE
SEQRES   8 A  545  PRO LEU PHE GLN THR ALA THR LEU SER SER GLU ASP CYS
SEQRES   9 A  545  LEU THR LEU ASN ILE GLN ARG PRO ALA GLY THR THR SER
SEQRES  10 A  545  ASN SER SER LEU PRO VAL LEU PHE TRP ILE PHE GLY GLY
SEQRES  11 A  545  GLY PHE GLU LEU GLY THR ASN GLN TYR TYR ASP GLY ILE
SEQRES  12 A  545  ASP LEU LEU THR GLU GLY ILE SER LEU GLY GLU PRO PHE
SEQRES  13 A  545  ILE PHE VAL ALA ILE ASN TYR ARG VAL GLY GLY PHE GLY
SEQRES  14 A  545  PHE LEU GLY GLY LYS GLU ILE LYS ALA ASP GLY SER SER
SEQRES  15 A  545  ASN LEU GLY LEU LEU ASP GLN ARG ILE ALA LEU GLU TRP
SEQRES  16 A  545  VAL ALA ASP ASN ILE ALA SER PHE GLY GLY ASP PRO SER
SEQRES  17 A  545  LYS VAL THR ILE TRP GLY GLU SER ALA GLY SER ILE SER
SEQRES  18 A  545  VAL PHE ASP GLN MET ALA LEU TYR GLY GLY ASN ASN LYS
SEQRES  19 A  545  TYR LYS GLY LYS ALA LEU PHE ARG GLY GLY ILE MET ASN
SEQRES  20 A  545  SER GLY SER VAL VAL PRO ALA ALA PRO VAL ASP GLY VAL
SEQRES  21 A  545  LYS ALA GLN ALA ILE TYR ASP HIS VAL VAL SER GLU ALA
SEQRES  22 A  545  GLY CYS ALA GLY THR SER ASP THR LEU ALA CYS LEU ARG
SEQRES  23 A  545  THR VAL ASP TYR THR LYS PHE LEU THR ALA VAL ASN SER
SEQRES  24 A  545  VAL PRO GLY ILE VAL SER TYR SER SER ILE ALA LEU SER
SEQRES  25 A  545  TYR LEU PRO ARG PRO ASP GLY VAL VAL LEU ILE ASP SER
SEQRES  26 A  545  PRO GLU GLU ILE VAL LYS ASN LYS GLN TYR ALA ALA VAL
SEQRES  27 A  545  PRO MET ILE ILE GLY ASP GLN GLU ASP GLU GLY THR LEU
SEQRES  28 A  545  PHE ALA VAL LEU PRO ASN ASN ILE THR SER THR ALA LYS
SEQRES  29 A  545  ILE VAL GLN TYR PHE GLN ASP LEU TYR PHE TYR ASN ALA
SEQRES  30 A  545  THR LYS GLU GLN LEU THR ALA PHE VAL ASN THR TYR PRO
SEQRES  31 A  545  THR ASP ILE THR ALA GLY SER PRO PHE ASN THR GLY ILE
SEQRES  32 A  545  PHE ASN GLU LEU TYR PRO GLY PHE LYS ARG LEU ALA ALA
SEQRES  33 A  545  ILE LEU GLY ASP MET THR PHE THR LEU ALA ARG ARG ALA
SEQRES  34 A  545  PHE LEU GLN LEU CYS SER GLU VAL ASN PRO ASP VAL PRO
SEQRES  35 A  545  SER TRP SER TYR LEU ALA SER TYR ASP TYR GLY PHE PRO
SEQRES  36 A  545  PHE LEU GLY THR PHE HIS ALA THR ASP ILE LEU GLN VAL
SEQRES  37 A  545  PHE TYR GLY VAL LEU PRO ASN TYR ALA SER GLY SER ILE
SEQRES  38 A  545  GLN LYS TYR TYR ILE ASN PHE VAL THR THR GLY ASP PRO
SEQRES  39 A  545  ASN LYS GLY ALA ALA VAL ASP ILE GLN TRP PRO GLN TRP
SEQRES  40 A  545  SER ALA LYS LYS ASN ILE LEU GLN ILE TYR ALA THR LYS
SEQRES  41 A  545  ALA VAL ILE VAL ALA ASP ASN PHE ARG ALA LYS SER TYR
SEQRES  42 A  545  GLU TYR LEU TYR ASN ASN ILE GLY ILE PHE ARG ILE
SEQRES   1 B  545  GLU ALA GLU ALA TYR VAL GLU PHE THR THR VAL ASN VAL
SEQRES   2 B  545  ASN TYR PRO GLU GLY GLU VAL VAL GLY VAL SER VAL LEU
SEQRES   3 B  545  GLY ILE GLU SER PHE ARG GLY VAL PRO PHE ALA GLN PRO
SEQRES   4 B  545  PRO VAL GLY ASN LEU ARG LEU LYS PRO PRO VAL ARG TYR
SEQRES   5 B  545  THR GLU ASN ILE GLY THR LYS ASP THR THR GLY ILE GLY
SEQRES   6 B  545  PRO SER CYS PRO GLN MET TYR LEU SER THR GLY ASN GLY
SEQRES   7 B  545  GLU LEU LEU PHE GLN LEU VAL GLY ASN LEU ILE ASN ILE
SEQRES   8 B  545  PRO LEU PHE GLN THR ALA THR LEU SER SER GLU ASP CYS
SEQRES   9 B  545  LEU THR LEU ASN ILE GLN ARG PRO ALA GLY THR THR SER
SEQRES  10 B  545  ASN SER SER LEU PRO VAL LEU PHE TRP ILE PHE GLY GLY
SEQRES  11 B  545  GLY PHE GLU LEU GLY THR ASN GLN TYR TYR ASP GLY ILE
SEQRES  12 B  545  ASP LEU LEU THR GLU GLY ILE SER LEU GLY GLU PRO PHE
SEQRES  13 B  545  ILE PHE VAL ALA ILE ASN TYR ARG VAL GLY GLY PHE GLY
SEQRES  14 B  545  PHE LEU GLY GLY LYS GLU ILE LYS ALA ASP GLY SER SER
SEQRES  15 B  545  ASN LEU GLY LEU LEU ASP GLN ARG ILE ALA LEU GLU TRP
SEQRES  16 B  545  VAL ALA ASP ASN ILE ALA SER PHE GLY GLY ASP PRO SER
SEQRES  17 B  545  LYS VAL THR ILE TRP GLY GLU SER ALA GLY SER ILE SER
SEQRES  18 B  545  VAL PHE ASP GLN MET ALA LEU TYR GLY GLY ASN ASN LYS
SEQRES  19 B  545  TYR LYS GLY LYS ALA LEU PHE ARG GLY GLY ILE MET ASN
SEQRES  20 B  545  SER GLY SER VAL VAL PRO ALA ALA PRO VAL ASP GLY VAL
SEQRES  21 B  545  LYS ALA GLN ALA ILE TYR ASP HIS VAL VAL SER GLU ALA
SEQRES  22 B  545  GLY CYS ALA GLY THR SER ASP THR LEU ALA CYS LEU ARG
SEQRES  23 B  545  THR VAL ASP TYR THR LYS PHE LEU THR ALA VAL ASN SER
SEQRES  24 B  545  VAL PRO GLY ILE VAL SER TYR SER SER ILE ALA LEU SER
SEQRES  25 B  545  TYR LEU PRO ARG PRO ASP GLY VAL VAL LEU ILE ASP SER
SEQRES  26 B  545  PRO GLU GLU ILE VAL LYS ASN LYS GLN TYR ALA ALA VAL
SEQRES  27 B  545  PRO MET ILE ILE GLY ASP GLN GLU ASP GLU GLY THR LEU
SEQRES  28 B  545  PHE ALA VAL LEU PRO ASN ASN ILE THR SER THR ALA LYS
SEQRES  29 B  545  ILE VAL GLN TYR PHE GLN ASP LEU TYR PHE TYR ASN ALA
SEQRES  30 B  545  THR LYS GLU GLN LEU THR ALA PHE VAL ASN THR TYR PRO
SEQRES  31 B  545  THR ASP ILE THR ALA GLY SER PRO PHE ASN THR GLY ILE
SEQRES  32 B  545  PHE ASN GLU LEU TYR PRO GLY PHE LYS ARG LEU ALA ALA
SEQRES  33 B  545  ILE LEU GLY ASP MET THR PHE THR LEU ALA ARG ARG ALA
SEQRES  34 B  545  PHE LEU GLN LEU CYS SER GLU VAL ASN PRO ASP VAL PRO
SEQRES  35 B  545  SER TRP SER TYR LEU ALA SER TYR ASP TYR GLY PHE PRO
SEQRES  36 B  545  PHE LEU GLY THR PHE HIS ALA THR ASP ILE LEU GLN VAL
SEQRES  37 B  545  PHE TYR GLY VAL LEU PRO ASN TYR ALA SER GLY SER ILE
SEQRES  38 B  545  GLN LYS TYR TYR ILE ASN PHE VAL THR THR GLY ASP PRO
SEQRES  39 B  545  ASN LYS GLY ALA ALA VAL ASP ILE GLN TRP PRO GLN TRP
SEQRES  40 B  545  SER ALA LYS LYS ASN ILE LEU GLN ILE TYR ALA THR LYS
SEQRES  41 B  545  ALA VAL ILE VAL ALA ASP ASN PHE ARG ALA LYS SER TYR
SEQRES  42 B  545  GLU TYR LEU TYR ASN ASN ILE GLY ILE PHE ARG ILE
HET    NAG  A1550      14
HET    NAG  A1551      14
HET    NAG  B1550      14
HET    7P9  A1552      28
HET    7P9  B1551      28
HET    1PE  A1553      16
HET    PGE  A1554      10
HET    PEG  A1555       7
HET    PGE  A1558      10
HET    PEG  A1559       7
HET    PEG  B1552       7
HET    PEG  B1553       7
HET    PEG  B1554       7
HET    PEG  B1555       7
HET    PGE  B1556      10
HET    GOL  A1556       6
HET    GOL  B1557       6
HET    GOL  B1558       6
HET    NO3  A1557       4
HET    NO3  B1559       4
HETNAM     NO3 NITRATE ION
HETNAM     7P9 [(2R)-2-HEPTANOYLOXY-3-PHOSPHONOOXY-PROPYL]
HETNAM   2 7P9  NONANOATE
HETNAM     1PE PENTAETHYLENE GLYCOL
HETNAM     PGE TRIETHYLENE GLYCOL
HETNAM     GOL GLYCEROL
HETNAM     PEG DI(HYDROXYETHYL)ETHER
HETNAM     NAG N-ACETYL-D-GLUCOSAMINE
HETSYN     1PE PEG400
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL
FORMUL   2  NO3    2(N O3 1-)
FORMUL   3  7P9    2(C19 H37 O8 P)
FORMUL   4  1PE    C10 H22 O6
FORMUL   5  PGE    3(C6 H14 O4)
FORMUL   6  GOL    3(C3 H8 O3)
FORMUL   7  PEG    6(C4 H10 O3)
FORMUL   8  NAG    3(C8 H15 N O6)
FORMUL   9  HOH   *768(H2 O)
HELIX    1   1 VAL A   45  ARG A   49  5                                   5
HELIX    2   2 GLU A   83  ILE A   93  1                                  11
HELIX    3   3 ILE A   95  THR A  102  1                                   8
HELIX    4   4 THR A  140  TYR A  144  5                                   5
HELIX    5   5 GLY A  146  LEU A  156  1                                  11
HELIX    6   6 VAL A  169  PHE A  174  1                                   6
HELIX    7   7 GLY A  177  GLY A  184  1                                   8
HELIX    8   8 ASN A  187  ILE A  204  1                                  18
HELIX    9   9 ALA A  205  PHE A  207  5                                   3
HELIX   10  10 SER A  220  ASN A  236  1                                  17
HELIX   11  11 GLY A  263  ALA A  277  1                                  15
HELIX   12  12 ASP A  284  ARG A  290  1                                   7
HELIX   13  13 ASP A  293  ASN A  302  1                                  10
HELIX   14  14 SER A  329  ASN A  336  1                                   8
HELIX   15  15 THR A  354  VAL A  358  5                                   5
HELIX   16  16 SER A  365  TYR A  377  1                                  13
HELIX   17  17 THR A  382  THR A  392  1                                  11
HELIX   18  18 ASP A  396  GLY A  400  5                                   5
HELIX   19  19 GLY A  414  PHE A  427  1                                  14
HELIX   20  20 PHE A  427  ASN A  442  1                                  16
HELIX   21  21 ALA A  466  VAL A  472  1                                   7
HELIX   22  22 ASN A  479  GLY A  496  1                                  18
HELIX   23  23 GLN A  510  LYS A  515  1                                   6
HELIX   24  24 ARG A  533  ASN A  543  1                                  11
HELIX   25  25 ILE A  544  ARG A  548  5                                   5
HELIX   26  26 VAL B   45  ARG B   49  5                                   5
HELIX   27  27 GLU B   83  ASN B   94  1                                  12
HELIX   28  28 ILE B   95  THR B  102  1                                   8
HELIX   29  29 THR B  140  TYR B  144  5                                   5
HELIX   30  30 GLY B  146  GLY B  157  1                                  12
HELIX   31  31 VAL B  169  PHE B  174  1                                   6
HELIX   32  32 GLY B  177  ASP B  183  1                                   7
HELIX   33  33 ASN B  187  ILE B  204  1                                  18
HELIX   34  34 ALA B  205  PHE B  207  5                                   3
HELIX   35  35 SER B  220  ASN B  236  1                                  17
HELIX   36  36 GLY B  263  ALA B  277  1                                  15
HELIX   37  37 ASP B  284  ARG B  290  1                                   7
HELIX   38  38 ASP B  293  ASN B  302  1                                  10
HELIX   39  39 SER B  329  ASN B  336  1                                   8
HELIX   40  40 GLY B  353  VAL B  358  1                                   6
HELIX   41  41 SER B  365  TYR B  377  1                                  13
HELIX   42  42 THR B  382  ASN B  391  1                                  10
HELIX   43  43 GLY B  414  PHE B  427  1                                  14
HELIX   44  44 PHE B  427  ASN B  442  1                                  16
HELIX   45  45 ALA B  466  VAL B  472  1                                   7
HELIX   46  46 ASN B  479  GLY B  496  1                                  18
HELIX   47  47 GLN B  510  LYS B  515  1                                   6
HELIX   48  48 ARG B  533  ASN B  543  1                                  11
HELIX   49  49 ILE B  544  ARG B  548  5                                   5
SHEET    1  AA 2 THR A  13  TYR A  19  0
SHEET    2  AA 2 GLY A  22  VAL A  29 -1  O  GLY A  22   N  TYR A  19
SHEET    1  AB 2 THR A  62  ASP A  64  0
SHEET    2  AB 2 GLY A  22  VAL A  29  1  O  GLU A  23   N  LYS A  63
SHEET    1  AC12 ALA A 525  ALA A 529  0
SHEET    2  AC12 ASN A 516  ILE A 520 -1  O  ILE A 517   N  VAL A 528
SHEET    3  AC12 SER A 447  ALA A 452  1  O  SER A 449   N  LEU A 518
SHEET    4  AC12 MET A 344  GLN A 349  1  O  MET A 344   N  TRP A 448
SHEET    5  AC12 GLY A 247  ASN A 251  1  O  GLY A 248   N  ILE A 345
SHEET    6  AC12 GLY A 209  GLU A 219  1  O  ILE A 216   N  ILE A 249
SHEET    7  AC12 LEU A 125  ILE A 131  1  O  LEU A 125   N  ASP A 210
SHEET    8  AC12 ILE A 161  ILE A 165  1  O  ILE A 161   N  LEU A 128
SHEET    9  AC12 THR A 110  PRO A 116 -1  O  ASN A 112   N  ALA A 164
SHEET   10  AC12 ILE A  32  PRO A  39 -1  O  GLU A  33   N  ARG A 115
SHEET   11  AC12 GLY A  22  VAL A  29 -1  O  VAL A  27   N  SER A  34
SHEET   12  AC12 THR A  62  ASP A  64  1  O  LYS A  63   N  VAL A  25
SHEET    1  AD12 ALA A 525  ALA A 529  0
SHEET    2  AD12 ASN A 516  ILE A 520 -1  O  ILE A 517   N  VAL A 528
SHEET    3  AD12 SER A 447  ALA A 452  1  O  SER A 449   N  LEU A 518
SHEET    4  AD12 MET A 344  GLN A 349  1  O  MET A 344   N  TRP A 448
SHEET    5  AD12 GLY A 247  ASN A 251  1  O  GLY A 248   N  ILE A 345
SHEET    6  AD12 GLY A 209  GLU A 219  1  O  ILE A 216   N  ILE A 249
SHEET    7  AD12 LEU A 125  ILE A 131  1  O  LEU A 125   N  ASP A 210
SHEET    8  AD12 ILE A 161  ILE A 165  1  O  ILE A 161   N  LEU A 128
SHEET    9  AD12 THR A 110  PRO A 116 -1  O  ASN A 112   N  ALA A 164
SHEET   10  AD12 ILE A  32  PRO A  39 -1  O  GLU A  33   N  ARG A 115
SHEET   11  AD12 GLY A  22  VAL A  29 -1  O  VAL A  27   N  SER A  34
SHEET   12  AD12 THR A  13  TYR A  19 -1  O  THR A  13   N  SER A  28
SHEET    1  AE 2 LYS A 238  TYR A 239  0
SHEET    2  AE 2 LYS A 242  ALA A 243 -1  O  LYS A 242   N  TYR A 239
SHEET    1  BA 2 THR B  14  TYR B  19  0
SHEET    2  BA 2 GLY B  22  VAL B  29 -1  O  GLY B  22   N  TYR B  19
SHEET    1  BB 2 THR B  62  ASP B  64  0
SHEET    2  BB 2 GLY B  22  VAL B  29  1  O  GLU B  23   N  LYS B  63
SHEET    1  BC12 ALA B 525  ALA B 529  0
SHEET    2  BC12 ASN B 516  ILE B 520 -1  O  ILE B 517   N  VAL B 528
SHEET    3  BC12 SER B 447  ALA B 452  1  O  SER B 449   N  LEU B 518
SHEET    4  BC12 MET B 344  GLN B 349  1  O  MET B 344   N  TRP B 448
SHEET    5  BC12 GLY B 247  ASN B 251  1  O  GLY B 248   N  ILE B 345
SHEET    6  BC12 GLY B 209  GLU B 219  1  O  ILE B 216   N  ILE B 249
SHEET    7  BC12 LEU B 125  ILE B 131  1  O  LEU B 125   N  ASP B 210
SHEET    8  BC12 ILE B 161  ILE B 165  1  O  ILE B 161   N  LEU B 128
SHEET    9  BC12 THR B 110  PRO B 116 -1  O  ASN B 112   N  ALA B 164
SHEET   10  BC12 ILE B  32  PRO B  39 -1  O  GLU B  33   N  ARG B 115
SHEET   11  BC12 GLY B  22  VAL B  29 -1  O  VAL B  27   N  SER B  34
SHEET   12  BC12 THR B  62  ASP B  64  1  O  LYS B  63   N  VAL B  25
SHEET    1  BD12 ALA B 525  ALA B 529  0
SHEET    2  BD12 ASN B 516  ILE B 520 -1  O  ILE B 517   N  VAL B 528
SHEET    3  BD12 SER B 447  ALA B 452  1  O  SER B 449   N  LEU B 518
SHEET    4  BD12 MET B 344  GLN B 349  1  O  MET B 344   N  TRP B 448
SHEET    5  BD12 GLY B 247  ASN B 251  1  O  GLY B 248   N  ILE B 345
SHEET    6  BD12 GLY B 209  GLU B 219  1  O  ILE B 216   N  ILE B 249
SHEET    7  BD12 LEU B 125  ILE B 131  1  O  LEU B 125   N  ASP B 210
SHEET    8  BD12 ILE B 161  ILE B 165  1  O  ILE B 161   N  LEU B 128
SHEET    9  BD12 THR B 110  PRO B 116 -1  O  ASN B 112   N  ALA B 164
SHEET   10  BD12 ILE B  32  PRO B  39 -1  O  GLU B  33   N  ARG B 115
SHEET   11  BD12 GLY B  22  VAL B  29 -1  O  VAL B  27   N  SER B  34
SHEET   12  BD12 THR B  14  TYR B  19 -1  O  VAL B  15   N  GLY B  26
SHEET    1  BE 2 LYS B 238  TYR B 239  0
SHEET    2  BE 2 LYS B 242  ALA B 243 -1  O  LYS B 242   N  TYR B 239
SSBOND   1 CYS A   72    CYS A  108                          1555   1555  2.05
SSBOND   2 CYS A  279    CYS A  288                          1555   1555  2.10
SSBOND   3 CYS B   72    CYS B  108                          1555   1555  2.04
SSBOND   4 CYS B  279    CYS B  288                          1555   1555  2.04
LINK         ND2 ASN A 362                 C1  NAG A1550     1555   1555  1.38
LINK         ND2 ASN A 380                 C1  NAG A1551     1555   1555  1.31
LINK         ND2 ASN B 362                 C1  NAG B1550     1555   1555  1.35
CISPEP   1 SER A  401    PRO A  402          0         4.28
CISPEP   2 VAL A  476    LEU A  477          0        -1.74
CISPEP   3 SER B  401    PRO B  402          0         5.37
CISPEP   4 VAL B  476    LEU B  477          0        -3.91
SITE     1 AC1 14 ASP A 455  PHE A 458  LEU A 470  GLN A 471
SITE     2 AC1 14 VAL A 476  LEU A 477  ASN A 479  ALA A 522
SITE     3 AC1 14 HOH A2462  HOH A2463  HOH A2464  HOH A2472
SITE     4 AC1 14 HOH A2528  HOH A2529
SITE     1 AC2  7 PHE A  98  ASP B 455  PHE B 458  LEU B 470
SITE     2 AC2  7 GLN B 471  VAL B 476  LEU B 477
SITE     1 AC3 13 GLY A 135  SER A 220  ALA A 221  ILE A 224
SITE     2 AC3 13 PRO A 257  ILE A 313  LEU A 315  MET A 425
SITE     3 AC3 13 THR A 426  PHE A 427  ILE A 544  PHE A 547
SITE     4 AC3 13 HOH A2184
SITE     1 AC4  1 TYR A 144
SITE     1 AC5  2 LEU A 355  HOH A2530
SITE     1 AC6  6 GLY B 135  SER B 220  ALA B 221  ILE B 224
SITE     2 AC6  6 THR B 426  HOH B2065
SITE     1 AC7  2 PEG B1554  PEG B1555
SITE     1 AC8  1 PEG B1553
SITE     1 AC9  2 PHE B 464  PEG B1553
SITE     1 BC1  1 LEU B 355
SITE     1 BC2  7 GLU A 350  PHE A 403  ASN A 404  LEU A 451
SITE     2 BC2  7 ARG A 533  HOH A2373  HOH A2374
SITE     1 BC3  5 GLU B 350  ASN B 404  LEU B 451  GLN B 519
SITE     2 BC3  5 ARG B 533
SITE     1 BC4  2 PHE B 408  ASN B 409
SITE     1 BC5  7 ARG A  36  ASP A  64  THR A  65  THR A  66
SITE     2 BC5  7 GLY A  67  ILE A  68  HOH A2531
SITE     1 BC6  3 ASN B  81  HIS B 272  SER B 303
SITE     1 BC7  4 TYR A 310  ASN A 362  HOH A2395  HOH A2525
SITE     1 BC8  6 ALA A 259  ASN A 380  GLU A 384  ARG A 548
SITE     2 BC8  6 HOH A2523  HOH A2527
SITE     1 BC9  3 TYR B 310  ASN B 362  HOH B2139
CRYST1  164.123  164.123   93.994  90.00  90.00  90.00 I 41         16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.006093  0.000000  0.000000        0.00000
SCALE2      0.000000  0.006093  0.000000        0.00000
SCALE3      0.000000  0.000000  0.010639        0.00000
TER    4179      ILE A 549
TER    8320      ILE B 549
MASTER      453    0   20   49   60    0   30    6 9295    2  223   84
END