longtext: 4BVL-pdb

content
HEADER    HYDROLASE                               26-JUN-13   4BVL
TITLE     STRUCTURE OF 202-208 DELETION MUTANT OF PHAZ7 PHB DEPOLYMERASE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: PHB DEPOLYMERASE PHAZ7;
COMPND   3 CHAIN: A, B, C, D;
COMPND   4 FRAGMENT: RESIDUES 39-380;
COMPND   5 EC: 3.1.1.75;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: PAUCIMONAS LEMOIGNEI;
SOURCE   3 ORGANISM_TAXID: 29443;
SOURCE   4 EXPRESSION_SYSTEM: BACILLUS SUBTILIS;
SOURCE   5 EXPRESSION_SYSTEM_TAXID: 1423;
SOURCE   6 EXPRESSION_SYSTEM_STRAIN: WB800
KEYWDS    HYDROLASE, CATALYTIC TRIAD, BIOPOLYMER BINDING
EXPDTA    X-RAY DIFFRACTION
AUTHOR    S.HERMAWAN,B.SUBEDI,A.C.PAPAGEORGIOU,D.JENDROSSEK
REVDAT   1   18-SEP-13 4BVL    0
JRNL        AUTH   D.JENDROSSEK,S.HERMAWAN,B.SUBEDI,A.C.PAPAGEORGIOU
JRNL        TITL   BIOCHEMICAL ANALYSIS AND STRUCTURE DETERMINATION OF
JRNL        TITL 2 PAUCIMONAS LEMOIGNEI POLY(3-HYDROXYBUTYRATE) (PHB)
JRNL        TITL 3 DEPOLYMERASE PHAZ7 MUTEINS REVEAL THE PHB BINDING SITE AND
JRNL        TITL 4 DETAILS OF SUBSTRATE-ENZYME INTERACTIONS.
JRNL        REF    MOL.MICROBIOL.                             2013
JRNL        REFN                   ESSN 1365-2958
JRNL        PMID   24007310
JRNL        DOI    10.1111/MMI.12391
REMARK   1
REMARK   1 REFERENCE 1
REMARK   1  AUTH   A.C.PAPAGEORGIOU,S.HERMAWAN,C.B.SINGH,D.JENDROSSEK
REMARK   1  TITL   STRUCTURAL BASIS OF POLY(3-HYDROXYBUTYRATE) HYDROLYSIS BY
REMARK   1  TITL 2 PHAZ7 DEPOLYMERASE FROM PAUCIMONAS LEMOIGNEI.
REMARK   1  REF    J.MOL.BIOL.                   V. 382  1184 2008
REMARK   1  REFN                   ISSN 0022-2836
REMARK   1  PMID   18706425
REMARK   1  DOI    10.1016/J.JMB.2008.07.078
REMARK   2
REMARK   2 RESOLUTION.    2.00 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.002
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.558
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.99
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.70
REMARK   3   NUMBER OF REFLECTIONS             : 74248
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.1190
REMARK   3   R VALUE            (WORKING SET) : 0.1152
REMARK   3   FREE R VALUE                     : 0.1908
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.0
REMARK   3   FREE R VALUE TEST SET COUNT      : 3711
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 19.5590 -  5.9524    0.95     2649   140  0.1582 0.2042
REMARK   3     2  5.9524 -  4.7468    0.98     2687   140  0.1128 0.1350
REMARK   3     3  4.7468 -  4.1533    0.99     2645   140  0.0940 0.1582
REMARK   3     4  4.1533 -  3.7766    0.99     2661   140  0.1004 0.1475
REMARK   3     5  3.7766 -  3.5075    0.99     2650   139  0.1105 0.1815
REMARK   3     6  3.5075 -  3.3018    1.00     2648   140  0.1171 0.2072
REMARK   3     7  3.3018 -  3.1371    1.00     2643   139  0.1226 0.2151
REMARK   3     8  3.1371 -  3.0011    1.00     2638   139  0.1183 0.1601
REMARK   3     9  3.0011 -  2.8859    1.00     2637   138  0.1181 0.1990
REMARK   3    10  2.8859 -  2.7866    1.00     2635   138  0.1190 0.1975
REMARK   3    11  2.7866 -  2.6997    1.00     2628   139  0.1138 0.1831
REMARK   3    12  2.6997 -  2.6227    1.00     2653   139  0.1127 0.1956
REMARK   3    13  2.6227 -  2.5538    1.00     2629   139  0.1106 0.1947
REMARK   3    14  2.5538 -  2.4917    1.00     2654   139  0.1140 0.2218
REMARK   3    15  2.4917 -  2.4351    1.00     2628   139  0.1142 0.1897
REMARK   3    16  2.4351 -  2.3834    1.00     2604   137  0.1052 0.1907
REMARK   3    17  2.3834 -  2.3358    1.00     2619   137  0.1042 0.2120
REMARK   3    18  2.3358 -  2.2918    1.00     2659   140  0.1047 0.1832
REMARK   3    19  2.2918 -  2.2509    1.00     2603   137  0.1085 0.2205
REMARK   3    20  2.2509 -  2.2128    1.00     2621   138  0.1113 0.1770
REMARK   3    21  2.2128 -  2.1772    1.00     2612   138  0.1114 0.2198
REMARK   3    22  2.1772 -  2.1437    1.00     2628   138  0.1182 0.2036
REMARK   3    23  2.1437 -  2.1122    1.00     2605   137  0.1165 0.1971
REMARK   3    24  2.1122 -  2.0825    1.00     2633   139  0.1218 0.2064
REMARK   3    25  2.0825 -  2.0544    1.00     2619   138  0.1236 0.2204
REMARK   3    26  2.0544 -  2.0277    1.00     2601   136  0.1327 0.2560
REMARK   3    27  2.0277 -  2.0024    0.78     2048   108  0.1684 0.2382
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.17
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 17.37
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 12.30
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 12.7
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.013          10237
REMARK   3   ANGLE     :  1.319          13883
REMARK   3   CHIRALITY :  0.076           1470
REMARK   3   PLANARITY :  0.007           1814
REMARK   3   DIHEDRAL  : 12.544           3487
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 4BVL COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 26-JUN-13.
REMARK 100 THE PDBE ID CODE IS EBI-57482.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 19-NOV-10
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 5.0
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : EMBL/DESY, HAMBURG
REMARK 200  BEAMLINE                       : X13
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.8123
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD (SX-165)
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XSCALE
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 74317
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.00
REMARK 200  RESOLUTION RANGE LOW       (A) : 19.60
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.0
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.7
REMARK 200  DATA REDUNDANCY                : 5.2
REMARK 200  R MERGE                    (I) : 0.11
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 12.50
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.12
REMARK 200  COMPLETENESS FOR SHELL     (%) : 94.9
REMARK 200  DATA REDUNDANCY IN SHELL       : 5.2
REMARK 200  R MERGE FOR SHELL          (I) : 0.33
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 4.40
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: PDB ENTRY 4BRS
REMARK 200
REMARK 200 REMARK: NONE
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 38
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: HANGING DROP VAPOUR DIFFUSION.
REMARK 280  PROTEIN CONCENTRATION 10 MG/ML. 0.1 M NAOAC (PH 5.0), 0.2
REMARK 280  M LICL, 15% W/V PEG6000
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z+1/2
REMARK 290       3555   -X,Y,-Z+1/2
REMARK 290       4555   X,-Y,-Z
REMARK 290       5555   X+1/2,Y+1/2,Z
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2
REMARK 290       8555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       86.18350
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       86.18350
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       46.61950
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       69.41450
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       46.61950
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       69.41450
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       86.18350
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       46.61950
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       69.41450
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       86.18350
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       46.61950
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       69.41450
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH A2205   LIES ON A SPECIAL POSITION.
REMARK 375      HOH C2176   LIES ON A SPECIAL POSITION.
REMARK 375      HOH D2417   LIES ON A SPECIAL POSITION.
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    PRO C    69     O    HOH C  2159              2.18
REMARK 500   OG   SER D   136     O    HOH D  2108              2.20
REMARK 500   O    ALA D   179     O    HOH D  2280              2.09
REMARK 500   O    HOH A  2099     O    HOH A  2100              2.12
REMARK 500   O    HOH A  2111     O    HOH A  2210              2.13
REMARK 500   O    HOH A  2126     O    HOH A  2297              2.10
REMARK 500   O    HOH A  2138     O    HOH A  2303              2.19
REMARK 500   O    HOH A  2141     O    HOH D  2397              2.09
REMARK 500   O    HOH A  2425     O    HOH A  2475              2.17
REMARK 500   O    HOH A  2491     O    HOH A  2492              2.20
REMARK 500   O    HOH B  2028     O    HOH B  2036              2.20
REMARK 500   O    HOH B  2040     O    HOH B  2080              2.18
REMARK 500   O    HOH B  2042     O    HOH B  2082              2.08
REMARK 500   O    HOH B  2053     O    HOH B  2054              2.17
REMARK 500   O    HOH B  2445     O    HOH B  2447              2.16
REMARK 500   O    HOH C  2067     O    HOH C  2068              2.15
REMARK 500   O    HOH C  2086     O    HOH C  2087              2.13
REMARK 500   O    HOH C  2132     O    HOH C  2274              2.20
REMARK 500   O    HOH C  2163     O    HOH C  2164              2.15
REMARK 500   O    HOH C  2276     O    HOH C  2429              2.15
REMARK 500   O    HOH C  2282     O    HOH C  2283              2.17
REMARK 500   O    HOH C  2312     O    HOH C  2315              2.14
REMARK 500   O    HOH C  2335     O    HOH C  2340              2.14
REMARK 500   O    HOH D  2022     O    HOH D  2074              2.19
REMARK 500   O    HOH D  2069     O    HOH D  2136              2.11
REMARK 500   O    HOH D  2135     O    HOH D  2271              2.17
REMARK 500   O    HOH D  2197     O    HOH D  2370              2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   ND2  ASN D    62     O    HOH B  2264     5445     2.09
REMARK 500   O    HOH A  2067     O    HOH D  2067     8445     2.19
REMARK 500   O    HOH C  2063     O    HOH A  2127     8545     2.06
REMARK 500   O    HOH D  2008     O    HOH D  2008     4545     2.19
REMARK 500   O    HOH D  2287     O    HOH A  2289     5545     2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ALA A  23       85.42   -150.97
REMARK 500    SER A 136     -130.82     55.90
REMARK 500    ALA A 181       72.58   -150.46
REMARK 500    GLU A 200     -138.33     56.84
REMARK 500    ARG A 228       66.30   -103.33
REMARK 500    PHE A 251       38.11   -141.23
REMARK 500    PHE B   9       -6.84     78.95
REMARK 500    SER B 136     -132.72     60.32
REMARK 500    ARG B 156      -65.06    -93.48
REMARK 500    ALA B 181       66.14   -154.57
REMARK 500    SER B 186     -156.22    -87.34
REMARK 500    GLU B 200     -143.12     56.97
REMARK 500    ARG B 228       72.52   -101.81
REMARK 500    SER C  55       -6.65    -59.39
REMARK 500    SER C 136     -132.72     56.19
REMARK 500    ARG C 156      -63.32    -94.41
REMARK 500    ALA C 181       59.32   -151.80
REMARK 500    SER C 186     -155.88    -83.49
REMARK 500    GLU C 200     -132.73     57.58
REMARK 500    ALA D  23       86.21   -152.02
REMARK 500    TYR D  66      -38.31   -135.66
REMARK 500    SER D 136     -136.53     57.19
REMARK 500    ARG D 156      -61.38    -91.94
REMARK 500    ALA D 181       67.54   -155.11
REMARK 500    SER D 186     -148.44    -92.41
REMARK 500    GLU D 200     -140.27     57.52
REMARK 500    ARG D 228       72.26   -109.17
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A2044        DISTANCE =  7.15 ANGSTROMS
REMARK 525    HOH A2234        DISTANCE =  6.21 ANGSTROMS
REMARK 525    HOH A2494        DISTANCE =  7.79 ANGSTROMS
REMARK 525    HOH B2247        DISTANCE =  7.17 ANGSTROMS
REMARK 525    HOH C2025        DISTANCE =  6.69 ANGSTROMS
REMARK 525    HOH C2066        DISTANCE =  5.64 ANGSTROMS
REMARK 525    HOH C2213        DISTANCE =  5.38 ANGSTROMS
REMARK 525    HOH C2447        DISTANCE =  5.38 ANGSTROMS
REMARK 525    HOH D2137        DISTANCE =  7.30 ANGSTROMS
REMARK 525    HOH D2139        DISTANCE =  6.80 ANGSTROMS
REMARK 525    HOH D2413        DISTANCE =  8.09 ANGSTROMS
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4BTV   RELATED DB: PDB
REMARK 900  STRUCTURE OF PHAZ7 PHB DEPOLYMERASE IN COMPLEX WITH
REMARK 900  3HB TRIMER
REMARK 900 RELATED ID: 4BVJ   RELATED DB: PDB
REMARK 900  STRUCTURE OF Y105A MUTANT OF PHAZ7 PHB DEPOLYMERASE
REMARK 900 RELATED ID: 4BVK   RELATED DB: PDB
REMARK 900  STRUCTURE OF Y190E MUTANT OF PHAZ7 PHB DEPOLYMERASE
REMARK 999
REMARK 999 SEQUENCE
REMARK 999 RESIDUES 202-208 HAVE BEEN DELETED
DBREF  4BVL A    1   342  UNP    Q939Q9   Q939Q9_PSELE    39    380
DBREF  4BVL B    1   342  UNP    Q939Q9   Q939Q9_PSELE    39    380
DBREF  4BVL C    1   342  UNP    Q939Q9   Q939Q9_PSELE    39    380
DBREF  4BVL D    1   342  UNP    Q939Q9   Q939Q9_PSELE    39    380
SEQADV 4BVL     A       UNP  Q939Q9    TRP   240 DELETION
SEQADV 4BVL     A       UNP  Q939Q9    TYR   241 DELETION
SEQADV 4BVL     A       UNP  Q939Q9    TYR   242 DELETION
SEQADV 4BVL     A       UNP  Q939Q9    GLY   243 DELETION
SEQADV 4BVL     A       UNP  Q939Q9    VAL   244 DELETION
SEQADV 4BVL     A       UNP  Q939Q9    TRP   245 DELETION
SEQADV 4BVL     A       UNP  Q939Q9    VAL   246 DELETION
SEQADV 4BVL     B       UNP  Q939Q9    TRP   240 DELETION
SEQADV 4BVL     B       UNP  Q939Q9    TYR   241 DELETION
SEQADV 4BVL     B       UNP  Q939Q9    TYR   242 DELETION
SEQADV 4BVL     B       UNP  Q939Q9    GLY   243 DELETION
SEQADV 4BVL     B       UNP  Q939Q9    VAL   244 DELETION
SEQADV 4BVL     B       UNP  Q939Q9    TRP   245 DELETION
SEQADV 4BVL     B       UNP  Q939Q9    VAL   246 DELETION
SEQADV 4BVL     C       UNP  Q939Q9    TRP   240 DELETION
SEQADV 4BVL     C       UNP  Q939Q9    TYR   241 DELETION
SEQADV 4BVL     C       UNP  Q939Q9    TYR   242 DELETION
SEQADV 4BVL     C       UNP  Q939Q9    GLY   243 DELETION
SEQADV 4BVL     C       UNP  Q939Q9    VAL   244 DELETION
SEQADV 4BVL     C       UNP  Q939Q9    TRP   245 DELETION
SEQADV 4BVL     C       UNP  Q939Q9    VAL   246 DELETION
SEQADV 4BVL     D       UNP  Q939Q9    TRP   240 DELETION
SEQADV 4BVL     D       UNP  Q939Q9    TYR   241 DELETION
SEQADV 4BVL     D       UNP  Q939Q9    TYR   242 DELETION
SEQADV 4BVL     D       UNP  Q939Q9    GLY   243 DELETION
SEQADV 4BVL     D       UNP  Q939Q9    VAL   244 DELETION
SEQADV 4BVL     D       UNP  Q939Q9    TRP   245 DELETION
SEQADV 4BVL     D       UNP  Q939Q9    VAL   246 DELETION
SEQRES   1 A  335  LEU THR CYS GLY THR ASN SER GLY PHE VAL CYS LYS GLY
SEQRES   2 A  335  THR GLN THR GLN TYR ALA GLY GLY PHE ALA PRO GLY VAL
SEQRES   3 A  335  GLY TYR GLY GLY PHE GLY GLY GLY SER CYS THR ALA THR
SEQRES   4 A  335  LYS THR PRO VAL ILE PHE ILE HIS GLY ASN GLY ASP ASN
SEQRES   5 A  335  ALA ILE SER PHE ASP MET PRO PRO GLY ASN VAL SER GLY
SEQRES   6 A  335  TYR GLY THR PRO ALA ARG SER VAL TYR ALA GLU LEU LYS
SEQRES   7 A  335  ALA ARG GLY TYR ASN ASP CYS GLU ILE PHE GLY VAL THR
SEQRES   8 A  335  TYR LEU SER SER SER GLU GLN GLY SER ALA GLN TYR ASN
SEQRES   9 A  335  TYR HIS SER SER THR LYS TYR ALA ILE ILE LYS THR PHE
SEQRES  10 A  335  ILE ASP LYS VAL LYS ALA TYR THR GLY LYS SER GLN VAL
SEQRES  11 A  335  ASP ILE VAL ALA HIS SER MET GLY VAL SER MET SER LEU
SEQRES  12 A  335  ALA THR LEU GLN TYR TYR ASN ASN TRP THR SER VAL ARG
SEQRES  13 A  335  LYS PHE ILE ASN LEU ALA GLY GLY ILE ARG GLY LEU TYR
SEQRES  14 A  335  SER CYS TYR TYR THR GLY TYR ALA ASN ALA ALA ALA PRO
SEQRES  15 A  335  THR CYS GLY SER GLN ASN TYR TYR ASN SER TYR THR PHE
SEQRES  16 A  335  GLY PHE PHE PRO GLU GLY SER ASN PRO TRP THR GLY SER
SEQRES  17 A  335  GLY SER THR ASN SER MET ARG ASP MET PRO ALA LYS ARG
SEQRES  18 A  335  THR ALA VAL SER PHE TYR THR LEU SER ALA GLY PHE LYS
SEQRES  19 A  335  ASP GLN VAL GLY CYS ALA THR ALA SER PHE TRP ALA GLY
SEQRES  20 A  335  CYS ASP SER ALA ALA LYS PHE ALA SER THR THR SER ASN
SEQRES  21 A  335  VAL LYS ALA GLN ILE ASN VAL GLY ALA GLY SER ASN ALA
SEQRES  22 A  335  THR GLN ALA ASP TYR ASP TRP ALA ASP GLY MET PRO TYR
SEQRES  23 A  335  ASN ALA GLY GLY GLY ASP THR THR ASN GLY VAL GLY HIS
SEQRES  24 A  335  PHE ARG THR LYS THR ASN THR GLY ALA ILE ILE GLN ARG
SEQRES  25 A  335  MET LEU LEU THR THR CYS THR GLY LEU ASP CYS ALA ALA
SEQRES  26 A  335  GLU TYR THR THR GLY PRO LYS ALA ALA TYR
SEQRES   1 B  335  LEU THR CYS GLY THR ASN SER GLY PHE VAL CYS LYS GLY
SEQRES   2 B  335  THR GLN THR GLN TYR ALA GLY GLY PHE ALA PRO GLY VAL
SEQRES   3 B  335  GLY TYR GLY GLY PHE GLY GLY GLY SER CYS THR ALA THR
SEQRES   4 B  335  LYS THR PRO VAL ILE PHE ILE HIS GLY ASN GLY ASP ASN
SEQRES   5 B  335  ALA ILE SER PHE ASP MET PRO PRO GLY ASN VAL SER GLY
SEQRES   6 B  335  TYR GLY THR PRO ALA ARG SER VAL TYR ALA GLU LEU LYS
SEQRES   7 B  335  ALA ARG GLY TYR ASN ASP CYS GLU ILE PHE GLY VAL THR
SEQRES   8 B  335  TYR LEU SER SER SER GLU GLN GLY SER ALA GLN TYR ASN
SEQRES   9 B  335  TYR HIS SER SER THR LYS TYR ALA ILE ILE LYS THR PHE
SEQRES  10 B  335  ILE ASP LYS VAL LYS ALA TYR THR GLY LYS SER GLN VAL
SEQRES  11 B  335  ASP ILE VAL ALA HIS SER MET GLY VAL SER MET SER LEU
SEQRES  12 B  335  ALA THR LEU GLN TYR TYR ASN ASN TRP THR SER VAL ARG
SEQRES  13 B  335  LYS PHE ILE ASN LEU ALA GLY GLY ILE ARG GLY LEU TYR
SEQRES  14 B  335  SER CYS TYR TYR THR GLY TYR ALA ASN ALA ALA ALA PRO
SEQRES  15 B  335  THR CYS GLY SER GLN ASN TYR TYR ASN SER TYR THR PHE
SEQRES  16 B  335  GLY PHE PHE PRO GLU GLY SER ASN PRO TRP THR GLY SER
SEQRES  17 B  335  GLY SER THR ASN SER MET ARG ASP MET PRO ALA LYS ARG
SEQRES  18 B  335  THR ALA VAL SER PHE TYR THR LEU SER ALA GLY PHE LYS
SEQRES  19 B  335  ASP GLN VAL GLY CYS ALA THR ALA SER PHE TRP ALA GLY
SEQRES  20 B  335  CYS ASP SER ALA ALA LYS PHE ALA SER THR THR SER ASN
SEQRES  21 B  335  VAL LYS ALA GLN ILE ASN VAL GLY ALA GLY SER ASN ALA
SEQRES  22 B  335  THR GLN ALA ASP TYR ASP TRP ALA ASP GLY MET PRO TYR
SEQRES  23 B  335  ASN ALA GLY GLY GLY ASP THR THR ASN GLY VAL GLY HIS
SEQRES  24 B  335  PHE ARG THR LYS THR ASN THR GLY ALA ILE ILE GLN ARG
SEQRES  25 B  335  MET LEU LEU THR THR CYS THR GLY LEU ASP CYS ALA ALA
SEQRES  26 B  335  GLU TYR THR THR GLY PRO LYS ALA ALA TYR
SEQRES   1 C  335  LEU THR CYS GLY THR ASN SER GLY PHE VAL CYS LYS GLY
SEQRES   2 C  335  THR GLN THR GLN TYR ALA GLY GLY PHE ALA PRO GLY VAL
SEQRES   3 C  335  GLY TYR GLY GLY PHE GLY GLY GLY SER CYS THR ALA THR
SEQRES   4 C  335  LYS THR PRO VAL ILE PHE ILE HIS GLY ASN GLY ASP ASN
SEQRES   5 C  335  ALA ILE SER PHE ASP MET PRO PRO GLY ASN VAL SER GLY
SEQRES   6 C  335  TYR GLY THR PRO ALA ARG SER VAL TYR ALA GLU LEU LYS
SEQRES   7 C  335  ALA ARG GLY TYR ASN ASP CYS GLU ILE PHE GLY VAL THR
SEQRES   8 C  335  TYR LEU SER SER SER GLU GLN GLY SER ALA GLN TYR ASN
SEQRES   9 C  335  TYR HIS SER SER THR LYS TYR ALA ILE ILE LYS THR PHE
SEQRES  10 C  335  ILE ASP LYS VAL LYS ALA TYR THR GLY LYS SER GLN VAL
SEQRES  11 C  335  ASP ILE VAL ALA HIS SER MET GLY VAL SER MET SER LEU
SEQRES  12 C  335  ALA THR LEU GLN TYR TYR ASN ASN TRP THR SER VAL ARG
SEQRES  13 C  335  LYS PHE ILE ASN LEU ALA GLY GLY ILE ARG GLY LEU TYR
SEQRES  14 C  335  SER CYS TYR TYR THR GLY TYR ALA ASN ALA ALA ALA PRO
SEQRES  15 C  335  THR CYS GLY SER GLN ASN TYR TYR ASN SER TYR THR PHE
SEQRES  16 C  335  GLY PHE PHE PRO GLU GLY SER ASN PRO TRP THR GLY SER
SEQRES  17 C  335  GLY SER THR ASN SER MET ARG ASP MET PRO ALA LYS ARG
SEQRES  18 C  335  THR ALA VAL SER PHE TYR THR LEU SER ALA GLY PHE LYS
SEQRES  19 C  335  ASP GLN VAL GLY CYS ALA THR ALA SER PHE TRP ALA GLY
SEQRES  20 C  335  CYS ASP SER ALA ALA LYS PHE ALA SER THR THR SER ASN
SEQRES  21 C  335  VAL LYS ALA GLN ILE ASN VAL GLY ALA GLY SER ASN ALA
SEQRES  22 C  335  THR GLN ALA ASP TYR ASP TRP ALA ASP GLY MET PRO TYR
SEQRES  23 C  335  ASN ALA GLY GLY GLY ASP THR THR ASN GLY VAL GLY HIS
SEQRES  24 C  335  PHE ARG THR LYS THR ASN THR GLY ALA ILE ILE GLN ARG
SEQRES  25 C  335  MET LEU LEU THR THR CYS THR GLY LEU ASP CYS ALA ALA
SEQRES  26 C  335  GLU TYR THR THR GLY PRO LYS ALA ALA TYR
SEQRES   1 D  335  LEU THR CYS GLY THR ASN SER GLY PHE VAL CYS LYS GLY
SEQRES   2 D  335  THR GLN THR GLN TYR ALA GLY GLY PHE ALA PRO GLY VAL
SEQRES   3 D  335  GLY TYR GLY GLY PHE GLY GLY GLY SER CYS THR ALA THR
SEQRES   4 D  335  LYS THR PRO VAL ILE PHE ILE HIS GLY ASN GLY ASP ASN
SEQRES   5 D  335  ALA ILE SER PHE ASP MET PRO PRO GLY ASN VAL SER GLY
SEQRES   6 D  335  TYR GLY THR PRO ALA ARG SER VAL TYR ALA GLU LEU LYS
SEQRES   7 D  335  ALA ARG GLY TYR ASN ASP CYS GLU ILE PHE GLY VAL THR
SEQRES   8 D  335  TYR LEU SER SER SER GLU GLN GLY SER ALA GLN TYR ASN
SEQRES   9 D  335  TYR HIS SER SER THR LYS TYR ALA ILE ILE LYS THR PHE
SEQRES  10 D  335  ILE ASP LYS VAL LYS ALA TYR THR GLY LYS SER GLN VAL
SEQRES  11 D  335  ASP ILE VAL ALA HIS SER MET GLY VAL SER MET SER LEU
SEQRES  12 D  335  ALA THR LEU GLN TYR TYR ASN ASN TRP THR SER VAL ARG
SEQRES  13 D  335  LYS PHE ILE ASN LEU ALA GLY GLY ILE ARG GLY LEU TYR
SEQRES  14 D  335  SER CYS TYR TYR THR GLY TYR ALA ASN ALA ALA ALA PRO
SEQRES  15 D  335  THR CYS GLY SER GLN ASN TYR TYR ASN SER TYR THR PHE
SEQRES  16 D  335  GLY PHE PHE PRO GLU GLY SER ASN PRO TRP THR GLY SER
SEQRES  17 D  335  GLY SER THR ASN SER MET ARG ASP MET PRO ALA LYS ARG
SEQRES  18 D  335  THR ALA VAL SER PHE TYR THR LEU SER ALA GLY PHE LYS
SEQRES  19 D  335  ASP GLN VAL GLY CYS ALA THR ALA SER PHE TRP ALA GLY
SEQRES  20 D  335  CYS ASP SER ALA ALA LYS PHE ALA SER THR THR SER ASN
SEQRES  21 D  335  VAL LYS ALA GLN ILE ASN VAL GLY ALA GLY SER ASN ALA
SEQRES  22 D  335  THR GLN ALA ASP TYR ASP TRP ALA ASP GLY MET PRO TYR
SEQRES  23 D  335  ASN ALA GLY GLY GLY ASP THR THR ASN GLY VAL GLY HIS
SEQRES  24 D  335  PHE ARG THR LYS THR ASN THR GLY ALA ILE ILE GLN ARG
SEQRES  25 D  335  MET LEU LEU THR THR CYS THR GLY LEU ASP CYS ALA ALA
SEQRES  26 D  335  GLU TYR THR THR GLY PRO LYS ALA ALA TYR
FORMUL   5  HOH   *1803(H2 O)
HELIX    1   1 ASN A   52  MET A   58  5                                   7
HELIX    2   2 SER A   72  ARG A   80  1                                   9
HELIX    3   3 SER A   94  SER A  100  1                                   7
HELIX    4   4 ALA A  101  ASN A  104  5                                   4
HELIX    5   5 SER A  107  GLY A  126  1                                  20
HELIX    6   6 SER A  136  ASN A  150  1                                  15
HELIX    7   7 ASN A  151  THR A  153  5                                   3
HELIX    8   8 SER A  170  GLY A  175  1                                   6
HELIX    9   9 ALA A  181  GLY A  185  5                                   5
HELIX   10  10 SER A  220  ARG A  222  5                                   3
HELIX   11  11 ASP A  223  ARG A  228  1                                   6
HELIX   12  12 ALA A  238  LYS A  241  5                                   4
HELIX   13  13 ASP A  242  ALA A  247  1                                   6
HELIX   14  14 GLY A  254  ALA A  258  5                                   5
HELIX   15  15 ASP A  286  GLY A  290  5                                   5
HELIX   16  16 PHE A  307  ASN A  312  1                                   6
HELIX   17  17 THR A  313  THR A  323  1                                  11
HELIX   18  18 THR A  326  ALA A  332  5                                   7
HELIX   19  19 ASN B   52  MET B   58  5                                   7
HELIX   20  20 SER B   72  ARG B   80  1                                   9
HELIX   21  21 SER B   94  SER B  100  1                                   7
HELIX   22  22 ALA B  101  ASN B  104  5                                   4
HELIX   23  23 SER B  107  GLY B  126  1                                  20
HELIX   24  24 SER B  136  ASN B  150  1                                  15
HELIX   25  25 ASN B  151  THR B  153  5                                   3
HELIX   26  26 SER B  170  GLY B  175  1                                   6
HELIX   27  27 ALA B  181  GLY B  185  5                                   5
HELIX   28  28 SER B  220  ARG B  222  5                                   3
HELIX   29  29 ASP B  223  ARG B  228  1                                   6
HELIX   30  30 ALA B  238  ASP B  242  5                                   5
HELIX   31  31 GLY B  254  ALA B  258  5                                   5
HELIX   32  32 ASP B  286  GLY B  290  5                                   5
HELIX   33  33 PHE B  307  ASN B  312  1                                   6
HELIX   34  34 THR B  313  THR B  323  1                                  11
HELIX   35  35 THR B  326  ALA B  332  5                                   7
HELIX   36  36 ASN C   52  MET C   58  5                                   7
HELIX   37  37 SER C   72  ARG C   80  1                                   9
HELIX   38  38 SER C   94  SER C  100  1                                   7
HELIX   39  39 ALA C  101  ASN C  104  5                                   4
HELIX   40  40 SER C  107  GLY C  126  1                                  20
HELIX   41  41 SER C  136  ASN C  150  1                                  15
HELIX   42  42 ASN C  151  THR C  153  5                                   3
HELIX   43  43 SER C  170  GLY C  175  1                                   6
HELIX   44  44 ALA C  181  GLY C  185  5                                   5
HELIX   45  45 SER C  220  ARG C  222  5                                   3
HELIX   46  46 ASP C  223  ARG C  228  1                                   6
HELIX   47  47 ALA C  238  ASP C  242  5                                   5
HELIX   48  48 GLY C  254  ALA C  258  5                                   5
HELIX   49  49 ASP C  286  GLY C  290  5                                   5
HELIX   50  50 PHE C  307  ASN C  312  1                                   6
HELIX   51  51 THR C  313  THR C  323  1                                  11
HELIX   52  52 THR C  326  ALA C  332  5                                   7
HELIX   53  53 ASN D   52  MET D   58  5                                   7
HELIX   54  54 SER D   72  ARG D   80  1                                   9
HELIX   55  55 SER D   94  SER D  100  1                                   7
HELIX   56  56 ALA D  101  ASN D  104  5                                   4
HELIX   57  57 SER D  107  GLY D  126  1                                  20
HELIX   58  58 SER D  136  ASN D  150  1                                  15
HELIX   59  59 ASN D  151  THR D  153  5                                   3
HELIX   60  60 SER D  170  GLY D  175  1                                   6
HELIX   61  61 ALA D  181  GLY D  185  5                                   5
HELIX   62  62 SER D  220  ARG D  222  5                                   3
HELIX   63  63 ASP D  223  ARG D  228  1                                   6
HELIX   64  64 ALA D  238  ASP D  242  5                                   5
HELIX   65  65 GLY D  254  ALA D  258  5                                   5
HELIX   66  66 ASP D  286  GLY D  290  5                                   5
HELIX   67  67 PHE D  307  ASN D  312  1                                   6
HELIX   68  68 THR D  313  THR D  323  1                                  11
HELIX   69  69 THR D  326  ALA D  332  5                                   7
SHEET    1  AA 9 THR A  16  TYR A  18  0
SHEET    2  AA 9 GLY A  30  GLY A  32  1  O  GLY A  30   N  GLN A  17
SHEET    3  AA 9 ILE A  87  VAL A  90 -1  O  GLY A  89   N  PHE A  31
SHEET    4  AA 9 VAL A  43  ILE A  46  1  O  VAL A  43   N  PHE A  88
SHEET    5  AA 9 VAL A 130  HIS A 135  1  O  ASP A 131   N  ILE A  44
SHEET    6  AA 9 VAL A 155  LEU A 161  1  N  ARG A 156   O  VAL A 130
SHEET    7  AA 9 SER A 232  SER A 237  1  O  SER A 232   N  PHE A 158
SHEET    8  AA 9 VAL A 268  ASN A 273  1  N  LYS A 269   O  PHE A 233
SHEET    9  AA 9 ALA A 340  ALA A 341 -1  O  ALA A 340   N  ASN A 273
SHEET    1  BA 2 ASN B   6  SER B   7  0
SHEET    2  BA 2 VAL B  10  CYS B  11 -1  O  VAL B  10   N  SER B   7
SHEET    1  BB 9 THR B  16  TYR B  18  0
SHEET    2  BB 9 GLY B  30  GLY B  32  1  O  GLY B  30   N  GLN B  17
SHEET    3  BB 9 ILE B  87  VAL B  90 -1  O  GLY B  89   N  PHE B  31
SHEET    4  BB 9 VAL B  43  ILE B  46  1  O  VAL B  43   N  PHE B  88
SHEET    5  BB 9 VAL B 130  HIS B 135  1  O  ASP B 131   N  ILE B  44
SHEET    6  BB 9 VAL B 155  LEU B 161  1  N  ARG B 156   O  VAL B 130
SHEET    7  BB 9 SER B 232  SER B 237  1  O  SER B 232   N  PHE B 158
SHEET    8  BB 9 VAL B 268  ASN B 273  1  N  LYS B 269   O  PHE B 233
SHEET    9  BB 9 ALA B 340  ALA B 341 -1  O  ALA B 340   N  ASN B 273
SHEET    1  CA 9 THR C  16  TYR C  18  0
SHEET    2  CA 9 GLY C  30  GLY C  32  1  O  GLY C  30   N  GLN C  17
SHEET    3  CA 9 ILE C  87  VAL C  90 -1  O  GLY C  89   N  PHE C  31
SHEET    4  CA 9 VAL C  43  ILE C  46  1  O  VAL C  43   N  PHE C  88
SHEET    5  CA 9 VAL C 130  HIS C 135  1  O  ASP C 131   N  ILE C  44
SHEET    6  CA 9 VAL C 155  LEU C 161  1  N  ARG C 156   O  VAL C 130
SHEET    7  CA 9 SER C 232  SER C 237  1  O  SER C 232   N  PHE C 158
SHEET    8  CA 9 VAL C 268  ASN C 273  1  N  LYS C 269   O  PHE C 233
SHEET    9  CA 9 ALA C 340  ALA C 341 -1  O  ALA C 340   N  ASN C 273
SHEET    1  DA 9 THR D  16  TYR D  18  0
SHEET    2  DA 9 GLY D  30  GLY D  32  1  O  GLY D  30   N  GLN D  17
SHEET    3  DA 9 ILE D  87  VAL D  90 -1  O  GLY D  89   N  PHE D  31
SHEET    4  DA 9 VAL D  43  ILE D  46  1  O  VAL D  43   N  PHE D  88
SHEET    5  DA 9 VAL D 130  HIS D 135  1  O  ASP D 131   N  ILE D  44
SHEET    6  DA 9 VAL D 155  LEU D 161  1  N  ARG D 156   O  VAL D 130
SHEET    7  DA 9 SER D 232  SER D 237  1  O  SER D 232   N  PHE D 158
SHEET    8  DA 9 VAL D 268  ASN D 273  1  N  LYS D 269   O  PHE D 233
SHEET    9  DA 9 ALA D 340  ALA D 341 -1  O  ALA D 340   N  ASN D 273
SSBOND   1 CYS A    3    CYS A   11                          1555   1555  2.03
SSBOND   2 CYS A   36    CYS A   85                          1555   1555  2.02
SSBOND   3 CYS A  171    CYS A  184                          1555   1555  2.02
SSBOND   4 CYS A  246    CYS A  255                          1555   1555  2.04
SSBOND   5 CYS A  325    CYS A  330                          1555   1555  2.04
SSBOND   6 CYS B    3    CYS B   11                          1555   1555  2.03
SSBOND   7 CYS B   36    CYS B   85                          1555   1555  2.05
SSBOND   8 CYS B  171    CYS B  184                          1555   1555  2.06
SSBOND   9 CYS B  246    CYS B  255                          1555   1555  2.08
SSBOND  10 CYS B  325    CYS B  330                          1555   1555  2.05
SSBOND  11 CYS C    3    CYS C   11                          1555   1555  2.03
SSBOND  12 CYS C   36    CYS C   85                          1555   1555  2.04
SSBOND  13 CYS C  171    CYS C  184                          1555   1555  2.07
SSBOND  14 CYS C  246    CYS C  255                          1555   1555  2.04
SSBOND  15 CYS C  325    CYS C  330                          1555   1555  2.05
SSBOND  16 CYS D    3    CYS D   11                          1555   1555  2.04
SSBOND  17 CYS D   36    CYS D   85                          1555   1555  2.04
SSBOND  18 CYS D  171    CYS D  184                          1555   1555  2.04
SSBOND  19 CYS D  246    CYS D  255                          1555   1555  2.08
SSBOND  20 CYS D  325    CYS D  330                          1555   1555  2.06
CRYST1   93.239  138.829  172.367  90.00  90.00  90.00 C 2 2 21     32
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.010725  0.000000  0.000000        0.00000
SCALE2      0.000000  0.007203  0.000000        0.00000
SCALE3      0.000000  0.000000  0.005802        0.00000
TER    2491      TYR A 342
TER    4979      TYR B 342
TER    7465      TYR C 342
TER    9959      TYR D 342
MASTER      416    0    0   69   38    0    0    611758    4   45  104
END