longtext: 4DGQ-pdb

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HEADER    OXIDOREDUCTASE                          26-JAN-12   4DGQ
TITLE     CRYSTAL STRUCTURE OF NON-HEME CHLOROPEROXIDASE FROM BURKHOLDERIA
TITLE    2 CENOCEPACIA
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: NON-HEME CHLOROPEROXIDASE;
COMPND   3 CHAIN: A, B, C;
COMPND   4 EC: 1.11.1.10;
COMPND   5 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: BURKHOLDERIA CENOCEPACIA;
SOURCE   3 ORGANISM_TAXID: 216591;
SOURCE   4 STRAIN: J2315 / LMG 16656;
SOURCE   5 GENE: CPO, BCEJ2315_07640, BCAL0771;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: AVA0421
KEYWDS    OXIDOREDUCTASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    A.S.GARDBERG,T.E.EDWARDS,J.A.ABENDROTH,B.STAKER,L.STEWART,SEATTLE
AUTHOR   2 STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE (SSGCID)
REVDAT   1   07-MAR-12 4DGQ    0
JRNL        AUTH   A.S.GARDBERG,T.E.EDWARDS,J.A.ABENDROTH,B.STAKER,L.STEWART
JRNL        TITL   CRYSTAL STRUCTURE OF NON-HEME CHLOROPEROXIDASE FROM
JRNL        TITL 2 BURKHOLDERIA CENOCEPACIA
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.85 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.6.0117
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.85
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 39.59
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8
REMARK   3   NUMBER OF REFLECTIONS             : 84695
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.136
REMARK   3   R VALUE            (WORKING SET) : 0.135
REMARK   3   FREE R VALUE                     : 0.163
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000
REMARK   3   FREE R VALUE TEST SET COUNT      : 4223
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.85
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.90
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 5786
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 98.12
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2140
REMARK   3   BIN FREE R VALUE SET COUNT          : 276
REMARK   3   BIN FREE R VALUE                    : 0.2430
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 6394
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 12
REMARK   3   SOLVENT ATOMS            : 858
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 19.59
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 13.90
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.02000
REMARK   3    B22 (A**2) : 0.00000
REMARK   3    B33 (A**2) : -0.01000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.01000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): NULL
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.092
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.056
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 3.610
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.967
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.954
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  6639 ; 0.016 ; 0.020
REMARK   3   BOND LENGTHS OTHERS               (A):  4350 ; 0.002 ; 0.020
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  9044 ; 1.595 ; 1.919
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 10566 ; 0.970 ; 3.000
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   840 ; 5.571 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   310 ;33.278 ;23.742
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   974 ;12.404 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    31 ;22.121 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   955 ; 0.106 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  7601 ; 0.009 ; 0.021
REMARK   3   GENERAL PLANES OTHERS             (A):  1427 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 9
REMARK   3
REMARK   3   TLS GROUP : 1
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A     1        A    23
REMARK   3    ORIGIN FOR THE GROUP (A): -22.5370   1.5280 -26.2990
REMARK   3    T TENSOR
REMARK   3      T11:   0.0435 T22:   0.0696
REMARK   3      T33:   0.0656 T12:   0.0050
REMARK   3      T13:  -0.0355 T23:   0.0355
REMARK   3    L TENSOR
REMARK   3      L11:   1.1876 L22:   0.9968
REMARK   3      L33:   2.6505 L12:  -0.3997
REMARK   3      L13:  -1.4292 L23:   0.8199
REMARK   3    S TENSOR
REMARK   3      S11:   0.0494 S12:   0.1399 S13:   0.1418
REMARK   3      S21:  -0.1889 S22:   0.0388 S23:   0.1570
REMARK   3      S31:  -0.0904 S32:  -0.1130 S33:  -0.0882
REMARK   3
REMARK   3   TLS GROUP : 2
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A    24        A   149
REMARK   3    ORIGIN FOR THE GROUP (A): -22.9180 -10.8590 -18.4650
REMARK   3    T TENSOR
REMARK   3      T11:   0.0203 T22:   0.0322
REMARK   3      T33:   0.0365 T12:  -0.0029
REMARK   3      T13:  -0.0221 T23:  -0.0090
REMARK   3    L TENSOR
REMARK   3      L11:   0.3226 L22:   0.3540
REMARK   3      L33:   0.3832 L12:   0.1038
REMARK   3      L13:   0.0419 L23:   0.0965
REMARK   3    S TENSOR
REMARK   3      S11:   0.0330 S12:   0.0429 S13:  -0.0160
REMARK   3      S21:  -0.0251 S22:  -0.0313 S23:   0.0740
REMARK   3      S31:   0.0334 S32:  -0.0563 S33:  -0.0017
REMARK   3
REMARK   3   TLS GROUP : 3
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A   150        A   276
REMARK   3    ORIGIN FOR THE GROUP (A): -15.1360 -17.4980 -16.3100
REMARK   3    T TENSOR
REMARK   3      T11:   0.0260 T22:   0.0092
REMARK   3      T33:   0.0263 T12:  -0.0039
REMARK   3      T13:  -0.0118 T23:  -0.0029
REMARK   3    L TENSOR
REMARK   3      L11:   0.5113 L22:   0.4585
REMARK   3      L33:   0.3387 L12:   0.1062
REMARK   3      L13:   0.0307 L23:  -0.0363
REMARK   3    S TENSOR
REMARK   3      S11:   0.0130 S12:   0.0183 S13:  -0.0501
REMARK   3      S21:  -0.0076 S22:  -0.0075 S23:   0.0129
REMARK   3      S31:   0.0798 S32:  -0.0001 S33:  -0.0055
REMARK   3
REMARK   3   TLS GROUP : 4
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B     1        B    23
REMARK   3    ORIGIN FOR THE GROUP (A): -24.3230  11.6470  -4.1100
REMARK   3    T TENSOR
REMARK   3      T11:   0.0261 T22:   0.0883
REMARK   3      T33:   0.0738 T12:   0.0177
REMARK   3      T13:  -0.0125 T23:  -0.0207
REMARK   3    L TENSOR
REMARK   3      L11:   2.1737 L22:   2.2385
REMARK   3      L33:   1.2311 L12:   1.2616
REMARK   3      L13:  -0.6289 L23:  -1.3087
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0371 S12:   0.1264 S13:   0.0989
REMARK   3      S21:  -0.0259 S22:   0.0412 S23:   0.1591
REMARK   3      S31:  -0.0550 S32:  -0.1995 S33:  -0.0041
REMARK   3
REMARK   3   TLS GROUP : 5
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B    24        B   149
REMARK   3    ORIGIN FOR THE GROUP (A): -13.5510  15.8230   4.9740
REMARK   3    T TENSOR
REMARK   3      T11:   0.0246 T22:   0.0130
REMARK   3      T33:   0.0369 T12:   0.0009
REMARK   3      T13:   0.0098 T23:  -0.0071
REMARK   3    L TENSOR
REMARK   3      L11:   0.3754 L22:   0.3331
REMARK   3      L33:   0.2991 L12:   0.2451
REMARK   3      L13:  -0.0585 L23:   0.1037
REMARK   3    S TENSOR
REMARK   3      S11:   0.0251 S12:  -0.0190 S13:   0.0469
REMARK   3      S21:   0.0206 S22:  -0.0142 S23:   0.0336
REMARK   3      S31:  -0.0427 S32:  -0.0259 S33:  -0.0109
REMARK   3
REMARK   3   TLS GROUP : 6
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B   150        B   276
REMARK   3    ORIGIN FOR THE GROUP (A):  -6.1560   9.3500   9.3180
REMARK   3    T TENSOR
REMARK   3      T11:   0.0125 T22:   0.0189
REMARK   3      T33:   0.0088 T12:  -0.0029
REMARK   3      T13:  -0.0035 T23:  -0.0060
REMARK   3    L TENSOR
REMARK   3      L11:   0.5888 L22:   0.5008
REMARK   3      L33:   0.5645 L12:   0.0079
REMARK   3      L13:  -0.1735 L23:   0.1912
REMARK   3    S TENSOR
REMARK   3      S11:   0.0120 S12:  -0.0787 S13:   0.0184
REMARK   3      S21:   0.0568 S22:   0.0080 S23:  -0.0232
REMARK   3      S31:   0.0073 S32:   0.0822 S33:  -0.0199
REMARK   3
REMARK   3   TLS GROUP : 7
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   C     1        C    23
REMARK   3    ORIGIN FOR THE GROUP (A):  -9.1780  21.8810 -20.8810
REMARK   3    T TENSOR
REMARK   3      T11:   0.0933 T22:   0.0353
REMARK   3      T33:   0.0833 T12:   0.0348
REMARK   3      T13:   0.0260 T23:   0.0324
REMARK   3    L TENSOR
REMARK   3      L11:   2.1411 L22:   2.4507
REMARK   3      L33:   1.4989 L12:   1.3535
REMARK   3      L13:   1.0233 L23:   1.1918
REMARK   3    S TENSOR
REMARK   3      S11:   0.0717 S12:   0.0658 S13:   0.1714
REMARK   3      S21:  -0.0358 S22:  -0.0302 S23:   0.1813
REMARK   3      S31:  -0.1903 S32:  -0.0981 S33:  -0.0415
REMARK   3
REMARK   3   TLS GROUP : 8
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   C    24        C   149
REMARK   3    ORIGIN FOR THE GROUP (A):   1.1590  14.9080 -28.8440
REMARK   3    T TENSOR
REMARK   3      T11:   0.0413 T22:   0.0523
REMARK   3      T33:   0.0289 T12:  -0.0068
REMARK   3      T13:   0.0042 T23:   0.0350
REMARK   3    L TENSOR
REMARK   3      L11:   0.2671 L22:   0.2290
REMARK   3      L33:   0.5125 L12:   0.0644
REMARK   3      L13:  -0.2904 L23:   0.0145
REMARK   3    S TENSOR
REMARK   3      S11:   0.0251 S12:   0.0601 S13:   0.0693
REMARK   3      S21:  -0.0400 S22:   0.0090 S23:   0.0192
REMARK   3      S31:  -0.0718 S32:   0.0120 S33:  -0.0341
REMARK   3
REMARK   3   TLS GROUP : 9
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   C   150        C   276
REMARK   3    ORIGIN FOR THE GROUP (A):   9.7580  10.2140 -25.1640
REMARK   3    T TENSOR
REMARK   3      T11:   0.0143 T22:   0.0535
REMARK   3      T33:   0.0229 T12:  -0.0104
REMARK   3      T13:   0.0098 T23:   0.0079
REMARK   3    L TENSOR
REMARK   3      L11:   0.3866 L22:   0.3472
REMARK   3      L33:   0.6584 L12:   0.0534
REMARK   3      L13:  -0.1826 L23:  -0.1488
REMARK   3    S TENSOR
REMARK   3      S11:   0.0177 S12:   0.0435 S13:  -0.0022
REMARK   3      S21:  -0.0475 S22:  -0.0038 S23:  -0.0597
REMARK   3      S31:  -0.0344 S32:   0.1004 S33:  -0.0140
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: U VALUES WITH TLS ADDED. HYDROGENS HAVE
REMARK   3  BEEN ADDED IN THE RIDING POSITIONS.
REMARK   4
REMARK   4 4DGQ COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 27-JAN-12.
REMARK 100 THE RCSB ID CODE IS RCSB070307.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 19-JAN-12
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : N
REMARK 200  RADIATION SOURCE               : ROTATING ANODE
REMARK 200  BEAMLINE                       : NULL
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU FR-E SUPERBRIGHT
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.541780
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU SATURN 944+
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XSCALE
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 84726
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.850
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.7
REMARK 200  DATA REDUNDANCY                : 11.100
REMARK 200  R MERGE                    (I) : 0.09900
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 20.7700
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.85
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.90
REMARK 200  COMPLETENESS FOR SHELL     (%) : 98.1
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.20
REMARK 200  R MERGE FOR SHELL          (I) : 0.50400
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 2.700
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MR
REMARK 200 SOFTWARE USED: PHASER_MR
REMARK 200 STARTING MODEL: PDB ENTRY 1ZOI
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 55.46
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.76
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: INTERNAL TRACKING NUMBER 228044H10.
REMARK 280  WIZARD III/IV SCREEN CONDITION H10: 30% 2-PROPANOL, 30% PEG3350,
REMARK 280  0.1 M TRIS, PH 8.5. BUCEA00095HA1 PS01264 AT 21.88MG/ML IN A
REMARK 280  STABILIZING BUFFER OF 25 MM HEPES, PH 7.0, 500 MM NACL, 2 MM DTT,
REMARK 280  0.025% SODIUM AZIDE, 5% GLYCEROL, VAPOR DIFFUSION, SITTING DROP,
REMARK 280  TEMPERATURE 290K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       55.92500
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 6070 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 27540 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -24.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     GLY A    -3
REMARK 465     PRO A    -2
REMARK 465     GLY A    -1
REMARK 465     GLY B    -3
REMARK 465     PRO B    -2
REMARK 465     GLY B    -1
REMARK 465     GLY C    -3
REMARK 465     PRO C    -2
REMARK 465     GLY C    -1
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     ARG A 113    CG   CD   NE   CZ   NH1  NH2
REMARK 470     LYS A 175    CG   CD   CE   NZ
REMARK 470     LYS B   7    CG   CD   CE   NZ
REMARK 470     LYS B 175    CG   CD   CE   NZ
REMARK 470     LYS B 244    CG   CD   CE   NZ
REMARK 470     LYS B 247    CG   CD   CE   NZ
REMARK 470     SER C   0    OG
REMARK 470     LYS C   7    CG   CD   CE   NZ
REMARK 470     ARG C 113    CG   CD   NE   CZ   NH1  NH2
REMARK 470     LYS C 175    CG   CD   CE   NZ
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OE2  GLU A   178     OH   TYR C   182              1.90
REMARK 500   OH   TYR B   182     OE2  GLU C   178              1.90
REMARK 500   OD1  ASP B    39     O    HOH B   552              2.01
REMARK 500   O    HOH B   637     O    HOH B   675              2.11
REMARK 500   O    HOH B   557     O    HOH B   644              2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    HIS A  26   CG    HIS A  26   CD2     0.100
REMARK 500    HIS A 262   CG    HIS A 262   CD2     0.061
REMARK 500    HIS B  26   CG    HIS B  26   CD2     0.076
REMARK 500    ASP B 234   CB    ASP B 234   CG      0.132
REMARK 500    HIS C  26   CG    HIS C  26   CD2     0.074
REMARK 500    HIS C  55   CG    HIS C  55   CD2     0.061
REMARK 500    HIS C  96   CG    HIS C  96   CD2     0.056
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ASP A   8   CB  -  CG  -  OD2 ANGL. DEV. =   5.5 DEGREES
REMARK 500    ASP A 264   CB  -  CG  -  OD2 ANGL. DEV. =   5.9 DEGREES
REMARK 500    ARG B  57   NE  -  CZ  -  NH1 ANGL. DEV. =   3.0 DEGREES
REMARK 500    ARG B  57   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.8 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PRO A  32       45.46   -105.02
REMARK 500    LEU A  33     -142.52    -96.46
REMARK 500    SER A  97     -121.37     64.09
REMARK 500    LEU A 127      108.66   -170.16
REMARK 500    ASN A 153       82.39   -163.83
REMARK 500    SER A 235      -94.28   -136.24
REMARK 500    PRO B  32       49.07   -102.15
REMARK 500    LEU B  33     -143.70   -102.66
REMARK 500    SER B  97     -124.92     63.46
REMARK 500    ASN B 153       77.40   -164.99
REMARK 500    SER B 235      -96.20   -136.33
REMARK 500    PRO C  32       48.48   -102.72
REMARK 500    LEU C  33     -141.90   -101.19
REMARK 500    SER C  97     -124.36     61.77
REMARK 500    LEU C 127      118.66   -164.33
REMARK 500    ASN C 153       79.41   -164.11
REMARK 500    SER C 235      -94.67   -137.11
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CHIRAL CENTERS
REMARK 500
REMARK 500 UNEXPECTED CONFIGURATION OF THE FOLLOWING CHIRAL
REMARK 500 CENTER(S) USING IMPROPER CA--C--CB--N CHIRALITY
REMARK 500 FOR AMINO ACIDS AND C1'--O4'--N1(N9)--C2' FOR
REMARK 500 NUCLEIC ACIDS OR EQUIVALENT ANGLE
REMARK 500 M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,6X,F5.1,6X,A1,10X,A1,3X,A16)
REMARK 500
REMARK 500  M RES CSSEQI    IMPROPER   EXPECTED   FOUND DETAILS
REMARK 500    ILE C 258        24.7      L          L   OUTSIDE RANGE
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EDO A 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EDO B 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EDO C 301
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: BUCEA.00095.H   RELATED DB: TARGETTRACK
DBREF  4DGQ A    1   276  UNP    B4EA96   B4EA96_BURCJ     1    276
DBREF  4DGQ B    1   276  UNP    B4EA96   B4EA96_BURCJ     1    276
DBREF  4DGQ C    1   276  UNP    B4EA96   B4EA96_BURCJ     1    276
SEQADV 4DGQ GLY A   -3  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ PRO A   -2  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ GLY A   -1  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ SER A    0  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ GLY B   -3  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ PRO B   -2  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ GLY B   -1  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ SER B    0  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ GLY C   -3  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ PRO C   -2  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ GLY C   -1  UNP  B4EA96              EXPRESSION TAG
SEQADV 4DGQ SER C    0  UNP  B4EA96              EXPRESSION TAG
SEQRES   1 A  280  GLY PRO GLY SER MET GLY THR VAL THR THR LYS ASP GLY
SEQRES   2 A  280  VAL GLU ILE PHE TYR LYS ASP TRP GLY PRO ARG ASP ALA
SEQRES   3 A  280  LYS VAL ILE HIS PHE HIS HIS GLY TRP PRO LEU SER SER
SEQRES   4 A  280  ASP ASP TRP ASP ALA GLN LEU LEU PHE PHE VAL ASN LYS
SEQRES   5 A  280  GLY PHE ARG VAL VAL ALA HIS ASP ARG ARG GLY HIS GLY
SEQRES   6 A  280  ARG SER SER GLN VAL TRP ASP GLY HIS ASP MET ASP HIS
SEQRES   7 A  280  TYR ALA ASP ASP ALA ALA ALA VAL VAL GLU LYS LEU GLY
SEQRES   8 A  280  THR HIS GLY ALA MET HIS VAL GLY HIS SER THR GLY GLY
SEQRES   9 A  280  GLY GLU VAL VAL ARG TYR ILE ALA ARG HIS GLY GLU ARG
SEQRES  10 A  280  ASN VAL SER LYS ALA VAL LEU ILE SER SER VAL PRO PRO
SEQRES  11 A  280  LEU MET VAL LYS THR SER SER ASN PRO ASN GLY THR PRO
SEQRES  12 A  280  LYS SER VAL PHE ASP ASP PHE GLN ALA HIS VAL ALA ALA
SEQRES  13 A  280  ASN ARG ALA GLN PHE TYR LEU ASP VAL PRO ALA GLY PRO
SEQRES  14 A  280  PHE TYR GLY TYR ASN ARG PRO GLY ALA LYS PRO SER GLU
SEQRES  15 A  280  GLY VAL ILE TYR ASN TRP TRP ARG GLN GLY MET MET GLY
SEQRES  16 A  280  SER THR LYS ALA GLN TYR ASP GLY ILE VAL ALA PHE SER
SEQRES  17 A  280  GLN THR ASP PHE THR ASN ASP LEU LYS GLY ILE THR ILE
SEQRES  18 A  280  PRO VAL LEU VAL ILE HIS GLY ASP ASP ASP GLN VAL VAL
SEQRES  19 A  280  PRO TYR ALA ASP SER GLY VAL LEU SER ALA LYS LEU VAL
SEQRES  20 A  280  LYS ASN GLY LYS LEU ILE THR TYR LYS GLY ALA PRO HIS
SEQRES  21 A  280  GLY ILE PRO THR THR HIS ALA ASP LYS VAL ASN ALA ASP
SEQRES  22 A  280  LEU LEU GLU PHE LEU GLN SER
SEQRES   1 B  280  GLY PRO GLY SER MET GLY THR VAL THR THR LYS ASP GLY
SEQRES   2 B  280  VAL GLU ILE PHE TYR LYS ASP TRP GLY PRO ARG ASP ALA
SEQRES   3 B  280  LYS VAL ILE HIS PHE HIS HIS GLY TRP PRO LEU SER SER
SEQRES   4 B  280  ASP ASP TRP ASP ALA GLN LEU LEU PHE PHE VAL ASN LYS
SEQRES   5 B  280  GLY PHE ARG VAL VAL ALA HIS ASP ARG ARG GLY HIS GLY
SEQRES   6 B  280  ARG SER SER GLN VAL TRP ASP GLY HIS ASP MET ASP HIS
SEQRES   7 B  280  TYR ALA ASP ASP ALA ALA ALA VAL VAL GLU LYS LEU GLY
SEQRES   8 B  280  THR HIS GLY ALA MET HIS VAL GLY HIS SER THR GLY GLY
SEQRES   9 B  280  GLY GLU VAL VAL ARG TYR ILE ALA ARG HIS GLY GLU ARG
SEQRES  10 B  280  ASN VAL SER LYS ALA VAL LEU ILE SER SER VAL PRO PRO
SEQRES  11 B  280  LEU MET VAL LYS THR SER SER ASN PRO ASN GLY THR PRO
SEQRES  12 B  280  LYS SER VAL PHE ASP ASP PHE GLN ALA HIS VAL ALA ALA
SEQRES  13 B  280  ASN ARG ALA GLN PHE TYR LEU ASP VAL PRO ALA GLY PRO
SEQRES  14 B  280  PHE TYR GLY TYR ASN ARG PRO GLY ALA LYS PRO SER GLU
SEQRES  15 B  280  GLY VAL ILE TYR ASN TRP TRP ARG GLN GLY MET MET GLY
SEQRES  16 B  280  SER THR LYS ALA GLN TYR ASP GLY ILE VAL ALA PHE SER
SEQRES  17 B  280  GLN THR ASP PHE THR ASN ASP LEU LYS GLY ILE THR ILE
SEQRES  18 B  280  PRO VAL LEU VAL ILE HIS GLY ASP ASP ASP GLN VAL VAL
SEQRES  19 B  280  PRO TYR ALA ASP SER GLY VAL LEU SER ALA LYS LEU VAL
SEQRES  20 B  280  LYS ASN GLY LYS LEU ILE THR TYR LYS GLY ALA PRO HIS
SEQRES  21 B  280  GLY ILE PRO THR THR HIS ALA ASP LYS VAL ASN ALA ASP
SEQRES  22 B  280  LEU LEU GLU PHE LEU GLN SER
SEQRES   1 C  280  GLY PRO GLY SER MET GLY THR VAL THR THR LYS ASP GLY
SEQRES   2 C  280  VAL GLU ILE PHE TYR LYS ASP TRP GLY PRO ARG ASP ALA
SEQRES   3 C  280  LYS VAL ILE HIS PHE HIS HIS GLY TRP PRO LEU SER SER
SEQRES   4 C  280  ASP ASP TRP ASP ALA GLN LEU LEU PHE PHE VAL ASN LYS
SEQRES   5 C  280  GLY PHE ARG VAL VAL ALA HIS ASP ARG ARG GLY HIS GLY
SEQRES   6 C  280  ARG SER SER GLN VAL TRP ASP GLY HIS ASP MET ASP HIS
SEQRES   7 C  280  TYR ALA ASP ASP ALA ALA ALA VAL VAL GLU LYS LEU GLY
SEQRES   8 C  280  THR HIS GLY ALA MET HIS VAL GLY HIS SER THR GLY GLY
SEQRES   9 C  280  GLY GLU VAL VAL ARG TYR ILE ALA ARG HIS GLY GLU ARG
SEQRES  10 C  280  ASN VAL SER LYS ALA VAL LEU ILE SER SER VAL PRO PRO
SEQRES  11 C  280  LEU MET VAL LYS THR SER SER ASN PRO ASN GLY THR PRO
SEQRES  12 C  280  LYS SER VAL PHE ASP ASP PHE GLN ALA HIS VAL ALA ALA
SEQRES  13 C  280  ASN ARG ALA GLN PHE TYR LEU ASP VAL PRO ALA GLY PRO
SEQRES  14 C  280  PHE TYR GLY TYR ASN ARG PRO GLY ALA LYS PRO SER GLU
SEQRES  15 C  280  GLY VAL ILE TYR ASN TRP TRP ARG GLN GLY MET MET GLY
SEQRES  16 C  280  SER THR LYS ALA GLN TYR ASP GLY ILE VAL ALA PHE SER
SEQRES  17 C  280  GLN THR ASP PHE THR ASN ASP LEU LYS GLY ILE THR ILE
SEQRES  18 C  280  PRO VAL LEU VAL ILE HIS GLY ASP ASP ASP GLN VAL VAL
SEQRES  19 C  280  PRO TYR ALA ASP SER GLY VAL LEU SER ALA LYS LEU VAL
SEQRES  20 C  280  LYS ASN GLY LYS LEU ILE THR TYR LYS GLY ALA PRO HIS
SEQRES  21 C  280  GLY ILE PRO THR THR HIS ALA ASP LYS VAL ASN ALA ASP
SEQRES  22 C  280  LEU LEU GLU PHE LEU GLN SER
HET    EDO  A 301       4
HET    EDO  B 301       4
HET    EDO  C 301       4
HETNAM     EDO 1,2-ETHANEDIOL
HETSYN     EDO ETHYLENE GLYCOL
FORMUL   4  EDO    3(C2 H6 O2)
FORMUL   7  HOH   *858(H2 O)
HELIX    1   1 SER A   34  ASP A   37  5                                   4
HELIX    2   2 TRP A   38  LYS A   48  1                                  11
HELIX    3   3 ASP A   71  GLY A   87  1                                  17
HELIX    4   4 THR A   98  GLY A  111  1                                  14
HELIX    5   5 GLU A  112  VAL A  115  5                                   4
HELIX    6   6 PRO A  139  GLY A  164  1                                  26
HELIX    7   7 SER A  177  GLY A  191  1                                  15
HELIX    8   8 SER A  192  THR A  206  1                                  15
HELIX    9   9 PHE A  208  GLY A  214  1                                   7
HELIX   10  10 PRO A  231  ASP A  234  5                                   4
HELIX   11  11 SER A  235  VAL A  243  1                                   9
HELIX   12  12 GLY A  257  HIS A  262  1                                   6
HELIX   13  13 HIS A  262  SER A  276  1                                  15
HELIX   14  14 SER B   34  ASP B   37  5                                   4
HELIX   15  15 TRP B   38  LYS B   48  1                                  11
HELIX   16  16 ASP B   71  GLY B   87  1                                  17
HELIX   17  17 THR B   98  GLY B  111  1                                  14
HELIX   18  18 PRO B  139  GLY B  164  1                                  26
HELIX   19  19 SER B  177  GLY B  191  1                                  15
HELIX   20  20 SER B  192  GLN B  205  1                                  14
HELIX   21  21 PHE B  208  GLY B  214  1                                   7
HELIX   22  22 PRO B  231  ASP B  234  5                                   4
HELIX   23  23 SER B  235  VAL B  243  1                                   9
HELIX   24  24 GLY B  257  HIS B  262  1                                   6
HELIX   25  25 HIS B  262  SER B  276  1                                  15
HELIX   26  26 SER C   34  ASP C   37  5                                   4
HELIX   27  27 TRP C   38  LYS C   48  1                                  11
HELIX   28  28 ASP C   71  GLY C   87  1                                  17
HELIX   29  29 THR C   98  GLY C  111  1                                  14
HELIX   30  30 GLU C  112  VAL C  115  5                                   4
HELIX   31  31 PRO C  139  GLY C  164  1                                  26
HELIX   32  32 SER C  177  GLY C  191  1                                  15
HELIX   33  33 SER C  192  THR C  206  1                                  15
HELIX   34  34 PHE C  208  GLY C  214  1                                   7
HELIX   35  35 PRO C  231  ASP C  234  5                                   4
HELIX   36  36 SER C  235  VAL C  243  1                                   9
HELIX   37  37 GLY C  257  HIS C  262  1                                   6
HELIX   38  38 HIS C  262  SER C  276  1                                  15
SHEET    1   A 8 THR A   3  THR A   5  0
SHEET    2   A 8 GLU A  11  TRP A  17 -1  O  ILE A  12   N  VAL A   4
SHEET    3   A 8 ARG A  51  HIS A  55 -1  O  VAL A  52   N  TRP A  17
SHEET    4   A 8 VAL A  24  HIS A  28  1  N  PHE A  27   O  VAL A  53
SHEET    5   A 8 MET A  92  HIS A  96  1  O  VAL A  94   N  HIS A  26
SHEET    6   A 8 LYS A 117  ILE A 121  1  O  ILE A 121   N  GLY A  95
SHEET    7   A 8 VAL A 219  GLY A 224  1  O  LEU A 220   N  LEU A 120
SHEET    8   A 8 GLY A 246  TYR A 251  1  O  LYS A 247   N  VAL A 221
SHEET    1   B 8 THR B   3  THR B   5  0
SHEET    2   B 8 GLU B  11  TRP B  17 -1  O  ILE B  12   N  VAL B   4
SHEET    3   B 8 ARG B  51  HIS B  55 -1  O  VAL B  52   N  TRP B  17
SHEET    4   B 8 VAL B  24  HIS B  28  1  N  PHE B  27   O  VAL B  53
SHEET    5   B 8 ALA B  91  HIS B  96  1  O  VAL B  94   N  HIS B  26
SHEET    6   B 8 VAL B 115  ILE B 121  1  O  ILE B 121   N  GLY B  95
SHEET    7   B 8 VAL B 219  GLY B 224  1  O  LEU B 220   N  LEU B 120
SHEET    8   B 8 GLY B 246  TYR B 251  1  O  ILE B 249   N  VAL B 221
SHEET    1   C 8 THR C   3  THR C   5  0
SHEET    2   C 8 GLU C  11  TRP C  17 -1  O  ILE C  12   N  VAL C   4
SHEET    3   C 8 ARG C  51  HIS C  55 -1  O  VAL C  52   N  TRP C  17
SHEET    4   C 8 VAL C  24  HIS C  28  1  N  PHE C  27   O  VAL C  53
SHEET    5   C 8 MET C  92  HIS C  96  1  O  VAL C  94   N  HIS C  26
SHEET    6   C 8 LYS C 117  ILE C 121  1  O  ILE C 121   N  GLY C  95
SHEET    7   C 8 VAL C 219  GLY C 224  1  O  ILE C 222   N  LEU C 120
SHEET    8   C 8 GLY C 246  TYR C 251  1  O  ILE C 249   N  VAL C 221
CISPEP   1 TRP A   31    PRO A   32          0        -5.55
CISPEP   2 PRO A  125    PRO A  126          0         3.88
CISPEP   3 TRP B   31    PRO B   32          0        -9.82
CISPEP   4 PRO B  125    PRO B  126          0         7.56
CISPEP   5 TRP C   31    PRO C   32          0       -10.65
CISPEP   6 PRO C  125    PRO C  126          0         4.12
SITE     1 AC1  4 TRP A  31  SER A  97  THR A  98  VAL A 229
SITE     1 AC2  5 TRP B  31  SER B  97  THR B  98  PHE B 203
SITE     2 AC2  5 VAL B 229
SITE     1 AC3  4 TRP C  31  SER C  97  THR C  98  VAL C 229
CRYST1   60.770  111.850   80.210  90.00 111.65  90.00 P 1 21 1      6
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.016455  0.000000  0.006533        0.00000
SCALE2      0.000000  0.008941  0.000000        0.00000
SCALE3      0.000000  0.000000  0.013414        0.00000
TER    2150      SER A 276
TER    4295      SER B 276
TER    6435      SER C 276
MASTER      552    0    3   38   24    0    4    6 7264    3   12   66
END