longtext: 4DMH-pdb

content
HEADER    HYDROLASE                               07-FEB-12   4DMH
TITLE     CRYSTAL STRUCTURE OF THE CFTR INHIBITORY FACTOR CIF WITH THE H207A
TITLE    2 MUTATION
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: PUTATIVE HYDROLASE;
COMPND   3 CHAIN: A, B, C, D;
COMPND   4 FRAGMENT: CIF (UNP RESIDUES 25-319);
COMPND   5 ENGINEERED: YES;
COMPND   6 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: PSEUDOMONAS AERUGINOSA;
SOURCE   3 ORGANISM_TAXID: 208963;
SOURCE   4 STRAIN: UCBPP-PA14;
SOURCE   5 GENE: PA14, PA14_26090;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: TOP10;
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PMQ70
KEYWDS    ALPHA BETA HYDROLASE, EPOXIDE HYDROLASE, SECRETED, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    C.D.BAHL,D.R.MADDEN
REVDAT   1   07-AUG-13 4DMH    0
JRNL        AUTH   C.D.BAHL,D.R.MADDEN
JRNL        TITL   CRYSTAL STRUCTURE OF THE CFTR INHIBITORY FACTOR CIF WITH THE
JRNL        TITL 2 H207A MUTATION
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.90 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.6.1_357)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER
REMARK   3               : ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : MLHL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.90
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 46.17
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 2.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.3
REMARK   3   NUMBER OF REFLECTIONS             : 94109
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.164
REMARK   3   R VALUE            (WORKING SET) : 0.162
REMARK   3   FREE R VALUE                     : 0.198
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000
REMARK   3   FREE R VALUE TEST SET COUNT      : 4706
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 46.1867 -  5.8999    0.98     3024   238  0.1663 0.1879
REMARK   3     2  5.8999 -  4.6843    0.99     3223     0  0.1404 10000000.0000
REMARK   3     3  4.6843 -  4.0926    0.98     2942   237  0.1256 0.1531
REMARK   3     4  4.0926 -  3.7186    0.98     2968   235  0.1445 0.1647
REMARK   3     5  3.7186 -  3.4522    0.98     3198     0  0.1514 10000000.0000
REMARK   3     6  3.4522 -  3.2487    0.98     2918   238  0.1531 0.1713
REMARK   3     7  3.2487 -  3.0860    0.98     2937   236  0.1596 0.1914
REMARK   3     8  3.0860 -  2.9517    0.98     3173     0  0.1757 10000000.0000
REMARK   3     9  2.9517 -  2.8381    0.98     2906   238  0.1680 0.2120
REMARK   3    10  2.8381 -  2.7402    0.98     2932   238  0.1570 0.2047
REMARK   3    11  2.7402 -  2.6545    0.98     3134     0  0.1602 10000000.0000
REMARK   3    12  2.6545 -  2.5786    0.98     2912   237  0.1620 0.1993
REMARK   3    13  2.5786 -  2.5108    0.98     2893   233  0.1613 0.2037
REMARK   3    14  2.5108 -  2.4495    0.97     3126     0  0.1652 10000000.0000
REMARK   3    15  2.4495 -  2.3938    0.97     2903   232  0.1569 0.2097
REMARK   3    16  2.3938 -  2.3429    0.97     2877   239  0.1564 0.1982
REMARK   3    17  2.3429 -  2.2960    0.97     3077     0  0.1554 10000000.0000
REMARK   3    18  2.2960 -  2.2527    0.97     2934   236  0.1525 0.2027
REMARK   3    19  2.2527 -  2.2125    0.97     2886   236  0.1512 0.2043
REMARK   3    20  2.2125 -  2.1750    0.97     3094     0  0.1580 10000000.0000
REMARK   3    21  2.1750 -  2.1399    0.97     2863   239  0.1536 0.1872
REMARK   3    22  2.1399 -  2.1070    0.97     2897   236  0.1497 0.2076
REMARK   3    23  2.1070 -  2.0760    0.97     3098     0  0.1560 10000000.0000
REMARK   3    24  2.0760 -  2.0467    0.97     2815   235  0.1588 0.2083
REMARK   3    25  2.0467 -  2.0191    0.97     2918   233  0.1593 0.2069
REMARK   3    26  2.0191 -  1.9928    0.96     3074     0  0.1648 10000000.0000
REMARK   3    27  1.9928 -  1.9679    0.97     2884   230  0.1655 0.2223
REMARK   3    28  1.9679 -  1.9442    0.96     2828   233  0.1622 0.2264
REMARK   3    29  1.9442 -  1.9216    0.96     3085     0  0.1679 10000000.0000
REMARK   3    30  1.9216 -  1.9000    0.96     2884   227  0.1775 0.2100
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : 0.40
REMARK   3   B_SOL              : 39.73
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.210
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 17.590
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : -1.31710
REMARK   3    B22 (A**2) : -2.05970
REMARK   3    B33 (A**2) : 3.37670
REMARK   3    B12 (A**2) : -0.00000
REMARK   3    B13 (A**2) : 0.36310
REMARK   3    B23 (A**2) : -0.00000
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.006           9809
REMARK   3   ANGLE     :  1.007          13306
REMARK   3   CHIRALITY :  0.074           1366
REMARK   3   PLANARITY :  0.005           1749
REMARK   3   DIHEDRAL  : 13.330           3580
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 4
REMARK   3   TLS GROUP : 1
REMARK   3    SELECTION: (chain A and resid 25:321)
REMARK   3    ORIGIN FOR THE GROUP (A): -22.0479  -3.4353  27.3738
REMARK   3    T TENSOR
REMARK   3      T11:   0.0290 T22:   0.0298
REMARK   3      T33:   0.0182 T12:   0.0016
REMARK   3      T13:   0.0070 T23:   0.0125
REMARK   3    L TENSOR
REMARK   3      L11:   0.4948 L22:   0.4905
REMARK   3      L33:   0.5721 L12:  -0.0050
REMARK   3      L13:   0.0976 L23:   0.0312
REMARK   3    S TENSOR
REMARK   3      S11:   0.0021 S12:  -0.0439 S13:  -0.0675
REMARK   3      S21:   0.0422 S22:  -0.0075 S23:   0.0533
REMARK   3      S31:   0.0765 S32:  -0.0333 S33:  -0.0003
REMARK   3   TLS GROUP : 2
REMARK   3    SELECTION: (chain B and resid 25:320)
REMARK   3    ORIGIN FOR THE GROUP (A): -31.1581  36.0790  15.7401
REMARK   3    T TENSOR
REMARK   3      T11:   0.0298 T22:   0.0218
REMARK   3      T33:   0.0500 T12:   0.0129
REMARK   3      T13:  -0.0176 T23:   0.0018
REMARK   3    L TENSOR
REMARK   3      L11:   0.6615 L22:   0.5273
REMARK   3      L33:   0.3315 L12:  -0.3863
REMARK   3      L13:  -0.0543 L23:  -0.1094
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0247 S12:   0.0194 S13:   0.0905
REMARK   3      S21:  -0.0180 S22:   0.0163 S23:  -0.0060
REMARK   3      S31:  -0.0310 S32:   0.0069 S33:   0.0009
REMARK   3   TLS GROUP : 3
REMARK   3    SELECTION: (chain C and resid 25:320)
REMARK   3    ORIGIN FOR THE GROUP (A):   5.7049  29.3870  27.1103
REMARK   3    T TENSOR
REMARK   3      T11:   0.0250 T22:   0.0197
REMARK   3      T33:   0.0398 T12:   0.0030
REMARK   3      T13:   0.0029 T23:  -0.0073
REMARK   3    L TENSOR
REMARK   3      L11:   0.4135 L22:   0.4731
REMARK   3      L33:   0.4224 L12:   0.0447
REMARK   3      L13:  -0.0123 L23:   0.0327
REMARK   3    S TENSOR
REMARK   3      S11:   0.0060 S12:  -0.0073 S13:   0.0981
REMARK   3      S21:   0.0199 S22:  -0.0052 S23:  -0.0442
REMARK   3      S31:  -0.0700 S32:   0.0110 S33:   0.0000
REMARK   3   TLS GROUP : 4
REMARK   3    SELECTION: (chain D and resid 25:321)
REMARK   3    ORIGIN FOR THE GROUP (A):  14.7536 -10.1605  15.7029
REMARK   3    T TENSOR
REMARK   3      T11:   0.0335 T22:   0.0335
REMARK   3      T33:   0.0066 T12:   0.0207
REMARK   3      T13:   0.0070 T23:  -0.0064
REMARK   3    L TENSOR
REMARK   3      L11:   0.6088 L22:   0.3949
REMARK   3      L33:   0.2488 L12:  -0.1582
REMARK   3      L13:  -0.0046 L23:   0.0929
REMARK   3    S TENSOR
REMARK   3      S11:   0.0062 S12:   0.0248 S13:  -0.0380
REMARK   3      S21:  -0.0101 S22:  -0.0068 S23:  -0.0671
REMARK   3      S31:   0.0266 S32:  -0.0029 S33:  -0.0081
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 4DMH COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 10-FEB-12.
REMARK 100 THE RCSB ID CODE IS RCSB070514.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 10-OCT-09
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : NSLS
REMARK 200  BEAMLINE                       : X6A
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9770
REMARK 200  MONOCHROMATOR                  : SI(111) CHANNEL CUT MONOCHROMATOR
REMARK 200  OPTICS                         : TOROIDAL FOCUSING MIRROR
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 270
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS-PACKAGE
REMARK 200  DATA SCALING SOFTWARE          : XDS-PACKAGE
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 94122
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.900
REMARK 200  RESOLUTION RANGE LOW       (A) : 46.180
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.3
REMARK 200  DATA REDUNDANCY                : 4.300
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : 0.07000
REMARK 200   FOR THE DATA SET  : 16.3000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.90
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.95
REMARK 200  COMPLETENESS FOR SHELL     (%) : 96.3
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.30
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : 0.33600
REMARK 200   FOR SHELL         : 4.400
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHENIX
REMARK 200 STARTING MODEL: CHAIN A OF PDB ENTRY 3KD2
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 46.10
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.28
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 13% PEG 8000, 0.125M CALCIUM CHLORIDE,
REMARK 280  0.1M SODIUM ACETATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE
REMARK 280  291K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y,-Z
REMARK 290       3555   X+1/2,Y+1/2,Z
REMARK 290       4555   -X+1/2,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       84.41950
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       41.88050
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       84.41950
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       41.88050
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 3350 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 21060 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -27.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 3340 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 20930 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -25.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH D 642  LIES ON A SPECIAL POSITION.
REMARK 375      HOH D 708  LIES ON A SPECIAL POSITION.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     HIS A   322
REMARK 465     HIS A   323
REMARK 465     HIS A   324
REMARK 465     HIS A   325
REMARK 465     HIS B   321
REMARK 465     HIS B   322
REMARK 465     HIS B   323
REMARK 465     HIS B   324
REMARK 465     HIS B   325
REMARK 465     HIS C   321
REMARK 465     HIS C   322
REMARK 465     HIS C   323
REMARK 465     HIS C   324
REMARK 465     HIS C   325
REMARK 465     HIS D   322
REMARK 465     HIS D   323
REMARK 465     HIS D   324
REMARK 465     HIS D   325
REMARK 480
REMARK 480 ZERO OCCUPANCY ATOM
REMARK 480 THE FOLLOWING RESIDUES HAVE ATOMS MODELED WITH ZERO
REMARK 480 OCCUPANCY. THE LOCATION AND PROPERTIES OF THESE ATOMS
REMARK 480 MAY NOT BE RELIABLE. (M=MODEL NUMBER; RES=RESIDUE NAME;
REMARK 480 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 480   M RES C SSEQI ATOMS
REMARK 480     ARG A  319   CB   CG   CD   NE   CZ   NH1  NH2
REMARK 480     ARG B  319   CB   CG   CD   NE   CZ   NH1  NH2
REMARK 480     ARG C  319   CB   CG   CD   NE   CZ   NH1  NH2
REMARK 480     ARG D  319   CB   CG   CD   NE   CZ   NH1  NH2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASP A 129     -137.60     61.94
REMARK 500    ALA A 154      142.66    178.58
REMARK 500    CYS A 303       54.09   -142.70
REMARK 500    ASP B 129     -137.69     60.49
REMARK 500    ALA B 154      141.54    177.04
REMARK 500    ASP B 185       19.47     58.45
REMARK 500    CYS B 303       56.11   -143.95
REMARK 500    ASP C 129     -137.74     61.77
REMARK 500    ALA C 154      143.22   -178.62
REMARK 500    CYS C 303       57.41   -141.60
REMARK 500    THR D  99      -64.76    -91.21
REMARK 500    ASP D 129     -137.43     59.23
REMARK 500    ALA D 154      142.60    176.89
REMARK 500    CYS D 303       56.14   -140.51
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A 401
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B 401
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL C 401
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL D 401
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 3KD2   RELATED DB: PDB
REMARK 900 SAME PROTEIN, BUT WITH THE WILD TYPE SEQUENCE
REMARK 900 RELATED ID: 3KDA   RELATED DB: PDB
REMARK 900 RELATED ID: 3PI6   RELATED DB: PDB
REMARK 900 RELATED ID: 4DLN   RELATED DB: PDB
REMARK 900 RELATED ID: 4DM7   RELATED DB: PDB
REMARK 900 RELATED ID: 4DMC   RELATED DB: PDB
REMARK 900 RELATED ID: 4DMF   RELATED DB: PDB
REMARK 900 RELATED ID: 4DMK   RELATED DB: PDB
REMARK 900 RELATED ID: 4DNF   RELATED DB: PDB
REMARK 900 RELATED ID: 4DNO   RELATED DB: PDB
DBREF  4DMH A   25   319  UNP    Q02P97   Q02P97_PSEAB    25    319
DBREF  4DMH B   25   319  UNP    Q02P97   Q02P97_PSEAB    25    319
DBREF  4DMH C   25   319  UNP    Q02P97   Q02P97_PSEAB    25    319
DBREF  4DMH D   25   319  UNP    Q02P97   Q02P97_PSEAB    25    319
SEQADV 4DMH ALA A  207  UNP  Q02P97    HIS   207 ENGINEERED MUTATION
SEQADV 4DMH HIS A  320  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS A  321  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS A  322  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS A  323  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS A  324  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS A  325  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH ALA B  207  UNP  Q02P97    HIS   207 ENGINEERED MUTATION
SEQADV 4DMH HIS B  320  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS B  321  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS B  322  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS B  323  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS B  324  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS B  325  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH ALA C  207  UNP  Q02P97    HIS   207 ENGINEERED MUTATION
SEQADV 4DMH HIS C  320  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS C  321  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS C  322  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS C  323  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS C  324  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS C  325  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH ALA D  207  UNP  Q02P97    HIS   207 ENGINEERED MUTATION
SEQADV 4DMH HIS D  320  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS D  321  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS D  322  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS D  323  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS D  324  UNP  Q02P97              EXPRESSION TAG
SEQADV 4DMH HIS D  325  UNP  Q02P97              EXPRESSION TAG
SEQRES   1 A  301  ALA GLU GLU PHE PRO VAL PRO ASN GLY PHE GLU SER ALA
SEQRES   2 A  301  TYR ARG GLU VAL ASP GLY VAL LYS LEU HIS TYR VAL LYS
SEQRES   3 A  301  GLY GLY GLN GLY PRO LEU VAL MET LEU VAL HIS GLY PHE
SEQRES   4 A  301  GLY GLN THR TRP TYR GLU TRP HIS GLN LEU MET PRO GLU
SEQRES   5 A  301  LEU ALA LYS ARG PHE THR VAL ILE ALA PRO ASP LEU PRO
SEQRES   6 A  301  GLY LEU GLY GLN SER GLU PRO PRO LYS THR GLY TYR SER
SEQRES   7 A  301  GLY GLU GLN VAL ALA VAL TYR LEU HIS LYS LEU ALA ARG
SEQRES   8 A  301  GLN PHE SER PRO ASP ARG PRO PHE ASP LEU VAL ALA HIS
SEQRES   9 A  301  ASP ILE GLY ILE TRP ASN THR TYR PRO MET VAL VAL LYS
SEQRES  10 A  301  ASN GLN ALA ASP ILE ALA ARG LEU VAL TYR MET GLU ALA
SEQRES  11 A  301  PRO ILE PRO ASP ALA ARG ILE TYR ARG PHE PRO ALA PHE
SEQRES  12 A  301  THR ALA GLN GLY GLU SER LEU VAL TRP HIS PHE SER PHE
SEQRES  13 A  301  PHE ALA ALA ASP ASP ARG LEU ALA GLU THR LEU ILE ALA
SEQRES  14 A  301  GLY LYS GLU ARG PHE PHE LEU GLU HIS PHE ILE LYS SER
SEQRES  15 A  301  ALA ALA SER ASN THR GLU VAL PHE SER GLU ARG LEU LEU
SEQRES  16 A  301  ASP LEU TYR ALA ARG SER TYR ALA LYS PRO HIS SER LEU
SEQRES  17 A  301  ASN ALA SER PHE GLU TYR TYR ARG ALA LEU ASN GLU SER
SEQRES  18 A  301  VAL ARG GLN ASN ALA GLU LEU ALA LYS THR ARG LEU GLN
SEQRES  19 A  301  MET PRO THR MET THR LEU ALA GLY GLY GLY HIS GLY GLY
SEQRES  20 A  301  MET GLY THR PHE GLN LEU GLU GLN MET LYS ALA TYR ALA
SEQRES  21 A  301  GLU ASP VAL GLU GLY HIS VAL LEU PRO GLY CYS GLY HIS
SEQRES  22 A  301  TRP LEU PRO GLU GLU CYS ALA ALA PRO MET ASN ARG LEU
SEQRES  23 A  301  VAL ILE ASP PHE LEU SER ARG GLY ARG HIS HIS HIS HIS
SEQRES  24 A  301  HIS HIS
SEQRES   1 B  301  ALA GLU GLU PHE PRO VAL PRO ASN GLY PHE GLU SER ALA
SEQRES   2 B  301  TYR ARG GLU VAL ASP GLY VAL LYS LEU HIS TYR VAL LYS
SEQRES   3 B  301  GLY GLY GLN GLY PRO LEU VAL MET LEU VAL HIS GLY PHE
SEQRES   4 B  301  GLY GLN THR TRP TYR GLU TRP HIS GLN LEU MET PRO GLU
SEQRES   5 B  301  LEU ALA LYS ARG PHE THR VAL ILE ALA PRO ASP LEU PRO
SEQRES   6 B  301  GLY LEU GLY GLN SER GLU PRO PRO LYS THR GLY TYR SER
SEQRES   7 B  301  GLY GLU GLN VAL ALA VAL TYR LEU HIS LYS LEU ALA ARG
SEQRES   8 B  301  GLN PHE SER PRO ASP ARG PRO PHE ASP LEU VAL ALA HIS
SEQRES   9 B  301  ASP ILE GLY ILE TRP ASN THR TYR PRO MET VAL VAL LYS
SEQRES  10 B  301  ASN GLN ALA ASP ILE ALA ARG LEU VAL TYR MET GLU ALA
SEQRES  11 B  301  PRO ILE PRO ASP ALA ARG ILE TYR ARG PHE PRO ALA PHE
SEQRES  12 B  301  THR ALA GLN GLY GLU SER LEU VAL TRP HIS PHE SER PHE
SEQRES  13 B  301  PHE ALA ALA ASP ASP ARG LEU ALA GLU THR LEU ILE ALA
SEQRES  14 B  301  GLY LYS GLU ARG PHE PHE LEU GLU HIS PHE ILE LYS SER
SEQRES  15 B  301  ALA ALA SER ASN THR GLU VAL PHE SER GLU ARG LEU LEU
SEQRES  16 B  301  ASP LEU TYR ALA ARG SER TYR ALA LYS PRO HIS SER LEU
SEQRES  17 B  301  ASN ALA SER PHE GLU TYR TYR ARG ALA LEU ASN GLU SER
SEQRES  18 B  301  VAL ARG GLN ASN ALA GLU LEU ALA LYS THR ARG LEU GLN
SEQRES  19 B  301  MET PRO THR MET THR LEU ALA GLY GLY GLY HIS GLY GLY
SEQRES  20 B  301  MET GLY THR PHE GLN LEU GLU GLN MET LYS ALA TYR ALA
SEQRES  21 B  301  GLU ASP VAL GLU GLY HIS VAL LEU PRO GLY CYS GLY HIS
SEQRES  22 B  301  TRP LEU PRO GLU GLU CYS ALA ALA PRO MET ASN ARG LEU
SEQRES  23 B  301  VAL ILE ASP PHE LEU SER ARG GLY ARG HIS HIS HIS HIS
SEQRES  24 B  301  HIS HIS
SEQRES   1 C  301  ALA GLU GLU PHE PRO VAL PRO ASN GLY PHE GLU SER ALA
SEQRES   2 C  301  TYR ARG GLU VAL ASP GLY VAL LYS LEU HIS TYR VAL LYS
SEQRES   3 C  301  GLY GLY GLN GLY PRO LEU VAL MET LEU VAL HIS GLY PHE
SEQRES   4 C  301  GLY GLN THR TRP TYR GLU TRP HIS GLN LEU MET PRO GLU
SEQRES   5 C  301  LEU ALA LYS ARG PHE THR VAL ILE ALA PRO ASP LEU PRO
SEQRES   6 C  301  GLY LEU GLY GLN SER GLU PRO PRO LYS THR GLY TYR SER
SEQRES   7 C  301  GLY GLU GLN VAL ALA VAL TYR LEU HIS LYS LEU ALA ARG
SEQRES   8 C  301  GLN PHE SER PRO ASP ARG PRO PHE ASP LEU VAL ALA HIS
SEQRES   9 C  301  ASP ILE GLY ILE TRP ASN THR TYR PRO MET VAL VAL LYS
SEQRES  10 C  301  ASN GLN ALA ASP ILE ALA ARG LEU VAL TYR MET GLU ALA
SEQRES  11 C  301  PRO ILE PRO ASP ALA ARG ILE TYR ARG PHE PRO ALA PHE
SEQRES  12 C  301  THR ALA GLN GLY GLU SER LEU VAL TRP HIS PHE SER PHE
SEQRES  13 C  301  PHE ALA ALA ASP ASP ARG LEU ALA GLU THR LEU ILE ALA
SEQRES  14 C  301  GLY LYS GLU ARG PHE PHE LEU GLU HIS PHE ILE LYS SER
SEQRES  15 C  301  ALA ALA SER ASN THR GLU VAL PHE SER GLU ARG LEU LEU
SEQRES  16 C  301  ASP LEU TYR ALA ARG SER TYR ALA LYS PRO HIS SER LEU
SEQRES  17 C  301  ASN ALA SER PHE GLU TYR TYR ARG ALA LEU ASN GLU SER
SEQRES  18 C  301  VAL ARG GLN ASN ALA GLU LEU ALA LYS THR ARG LEU GLN
SEQRES  19 C  301  MET PRO THR MET THR LEU ALA GLY GLY GLY HIS GLY GLY
SEQRES  20 C  301  MET GLY THR PHE GLN LEU GLU GLN MET LYS ALA TYR ALA
SEQRES  21 C  301  GLU ASP VAL GLU GLY HIS VAL LEU PRO GLY CYS GLY HIS
SEQRES  22 C  301  TRP LEU PRO GLU GLU CYS ALA ALA PRO MET ASN ARG LEU
SEQRES  23 C  301  VAL ILE ASP PHE LEU SER ARG GLY ARG HIS HIS HIS HIS
SEQRES  24 C  301  HIS HIS
SEQRES   1 D  301  ALA GLU GLU PHE PRO VAL PRO ASN GLY PHE GLU SER ALA
SEQRES   2 D  301  TYR ARG GLU VAL ASP GLY VAL LYS LEU HIS TYR VAL LYS
SEQRES   3 D  301  GLY GLY GLN GLY PRO LEU VAL MET LEU VAL HIS GLY PHE
SEQRES   4 D  301  GLY GLN THR TRP TYR GLU TRP HIS GLN LEU MET PRO GLU
SEQRES   5 D  301  LEU ALA LYS ARG PHE THR VAL ILE ALA PRO ASP LEU PRO
SEQRES   6 D  301  GLY LEU GLY GLN SER GLU PRO PRO LYS THR GLY TYR SER
SEQRES   7 D  301  GLY GLU GLN VAL ALA VAL TYR LEU HIS LYS LEU ALA ARG
SEQRES   8 D  301  GLN PHE SER PRO ASP ARG PRO PHE ASP LEU VAL ALA HIS
SEQRES   9 D  301  ASP ILE GLY ILE TRP ASN THR TYR PRO MET VAL VAL LYS
SEQRES  10 D  301  ASN GLN ALA ASP ILE ALA ARG LEU VAL TYR MET GLU ALA
SEQRES  11 D  301  PRO ILE PRO ASP ALA ARG ILE TYR ARG PHE PRO ALA PHE
SEQRES  12 D  301  THR ALA GLN GLY GLU SER LEU VAL TRP HIS PHE SER PHE
SEQRES  13 D  301  PHE ALA ALA ASP ASP ARG LEU ALA GLU THR LEU ILE ALA
SEQRES  14 D  301  GLY LYS GLU ARG PHE PHE LEU GLU HIS PHE ILE LYS SER
SEQRES  15 D  301  ALA ALA SER ASN THR GLU VAL PHE SER GLU ARG LEU LEU
SEQRES  16 D  301  ASP LEU TYR ALA ARG SER TYR ALA LYS PRO HIS SER LEU
SEQRES  17 D  301  ASN ALA SER PHE GLU TYR TYR ARG ALA LEU ASN GLU SER
SEQRES  18 D  301  VAL ARG GLN ASN ALA GLU LEU ALA LYS THR ARG LEU GLN
SEQRES  19 D  301  MET PRO THR MET THR LEU ALA GLY GLY GLY HIS GLY GLY
SEQRES  20 D  301  MET GLY THR PHE GLN LEU GLU GLN MET LYS ALA TYR ALA
SEQRES  21 D  301  GLU ASP VAL GLU GLY HIS VAL LEU PRO GLY CYS GLY HIS
SEQRES  22 D  301  TRP LEU PRO GLU GLU CYS ALA ALA PRO MET ASN ARG LEU
SEQRES  23 D  301  VAL ILE ASP PHE LEU SER ARG GLY ARG HIS HIS HIS HIS
SEQRES  24 D  301  HIS HIS
HET    GOL  A 401       6
HET    GOL  B 401       6
HET    GOL  C 401       6
HET    GOL  D 401       6
HETNAM     GOL GLYCEROL
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL
FORMUL   5  GOL    4(C3 H8 O3)
FORMUL   9  HOH   *824(H2 O)
HELIX    1   1 THR A   66  HIS A   71  5                                   6
HELIX    2   2 LEU A   73  ALA A   78  1                                   6
HELIX    3   3 SER A  102  SER A  118  1                                  17
HELIX    4   4 ASP A  129  ASN A  134  1                                   6
HELIX    5   5 THR A  135  ASN A  142  1                                   8
HELIX    6   6 ASP A  158  PHE A  164  5                                   7
HELIX    7   7 TRP A  176  ALA A  183  1                                   8
HELIX    8   8 ARG A  186  ALA A  193  1                                   8
HELIX    9   9 LYS A  195  ALA A  207  1                                  13
HELIX   10  10 ASN A  210  PHE A  214  5                                   5
HELIX   11  11 SER A  215  ALA A  227  1                                  13
HELIX   12  12 LYS A  228  ALA A  241  1                                  14
HELIX   13  13 ALA A  241  ALA A  253  1                                  13
HELIX   14  14 THR A  274  ALA A  284  1                                  11
HELIX   15  15 TRP A  298  CYS A  303  1                                   6
HELIX   16  16 CYS A  303  SER A  316  1                                  14
HELIX   17  17 THR B   66  HIS B   71  5                                   6
HELIX   18  18 GLN B   72  ALA B   78  1                                   7
HELIX   19  19 SER B  102  SER B  118  1                                  17
HELIX   20  20 ASP B  129  ASN B  134  1                                   6
HELIX   21  21 THR B  135  ASN B  142  1                                   8
HELIX   22  22 ASP B  158  PHE B  164  5                                   7
HELIX   23  23 TRP B  176  ALA B  183  1                                   8
HELIX   24  24 ARG B  186  ALA B  193  1                                   8
HELIX   25  25 LYS B  195  ALA B  207  1                                  13
HELIX   26  26 ASN B  210  PHE B  214  5                                   5
HELIX   27  27 SER B  215  ALA B  227  1                                  13
HELIX   28  28 LYS B  228  ALA B  241  1                                  14
HELIX   29  29 ALA B  241  ALA B  253  1                                  13
HELIX   30  30 THR B  274  ALA B  282  1                                   9
HELIX   31  31 TRP B  298  CYS B  303  1                                   6
HELIX   32  32 CYS B  303  ARG B  317  1                                  15
HELIX   33  33 THR C   66  HIS C   71  5                                   6
HELIX   34  34 GLN C   72  ALA C   78  1                                   7
HELIX   35  35 SER C  102  SER C  118  1                                  17
HELIX   36  36 ASP C  129  ASN C  134  1                                   6
HELIX   37  37 THR C  135  ASN C  142  1                                   8
HELIX   38  38 ASP C  158  PHE C  164  5                                   7
HELIX   39  39 VAL C  175  ALA C  183  1                                   9
HELIX   40  40 ARG C  186  ALA C  193  1                                   8
HELIX   41  41 LYS C  195  ALA C  207  1                                  13
HELIX   42  42 ASN C  210  PHE C  214  5                                   5
HELIX   43  43 SER C  215  ALA C  227  1                                  13
HELIX   44  44 LYS C  228  ALA C  241  1                                  14
HELIX   45  45 ALA C  241  ALA C  253  1                                  13
HELIX   46  46 THR C  274  ALA C  284  1                                  11
HELIX   47  47 TRP C  298  CYS C  303  1                                   6
HELIX   48  48 CYS C  303  SER C  316  1                                  14
HELIX   49  49 THR D   66  HIS D   71  5                                   6
HELIX   50  50 GLN D   72  ALA D   78  1                                   7
HELIX   51  51 SER D  102  SER D  118  1                                  17
HELIX   52  52 ASP D  129  ASN D  134  1                                   6
HELIX   53  53 THR D  135  ASN D  142  1                                   8
HELIX   54  54 ASP D  158  PHE D  164  5                                   7
HELIX   55  55 VAL D  175  ALA D  183  1                                   9
HELIX   56  56 ARG D  186  ALA D  193  1                                   8
HELIX   57  57 LYS D  195  ALA D  207  1                                  13
HELIX   58  58 ASN D  210  PHE D  214  5                                   5
HELIX   59  59 SER D  215  ALA D  227  1                                  13
HELIX   60  60 LYS D  228  ALA D  241  1                                  14
HELIX   61  61 ALA D  241  ALA D  253  1                                  13
HELIX   62  62 THR D  274  ALA D  284  1                                  11
HELIX   63  63 TRP D  298  CYS D  303  1                                   6
HELIX   64  64 CYS D  303  ARG D  317  1                                  15
SHEET    1   A 8 GLU A  35  VAL A  41  0
SHEET    2   A 8 VAL A  44  GLY A  52 -1  O  VAL A  44   N  VAL A  41
SHEET    3   A 8 THR A  82  PRO A  86 -1  O  VAL A  83   N  GLY A  51
SHEET    4   A 8 LEU A  56  VAL A  60  1  N  VAL A  57   O  THR A  82
SHEET    5   A 8 PHE A 123  HIS A 128  1  O  VAL A 126   N  VAL A  60
SHEET    6   A 8 ILE A 146  MET A 152  1  O  VAL A 150   N  LEU A 125
SHEET    7   A 8 THR A 261  GLY A 266  1  O  MET A 262   N  LEU A 149
SHEET    8   A 8 VAL A 287  LEU A 292  1  O  LEU A 292   N  ALA A 265
SHEET    1   B 8 PHE B  34  VAL B  41  0
SHEET    2   B 8 VAL B  44  GLY B  52 -1  O  LEU B  46   N  ARG B  39
SHEET    3   B 8 THR B  82  PRO B  86 -1  O  VAL B  83   N  GLY B  51
SHEET    4   B 8 LEU B  56  VAL B  60  1  N  VAL B  57   O  THR B  82
SHEET    5   B 8 PHE B 123  HIS B 128  1  O  VAL B 126   N  VAL B  60
SHEET    6   B 8 ILE B 146  MET B 152  1  O  VAL B 150   N  LEU B 125
SHEET    7   B 8 THR B 261  GLY B 266  1  O  MET B 262   N  LEU B 149
SHEET    8   B 8 VAL B 287  LEU B 292  1  O  LEU B 292   N  ALA B 265
SHEET    1   C 2 PHE B 167  THR B 168  0
SHEET    2   C 2 GLY B 171  GLU B 172 -1  O  GLY B 171   N  THR B 168
SHEET    1   D 8 GLU C  35  VAL C  41  0
SHEET    2   D 8 VAL C  44  GLY C  52 -1  O  VAL C  44   N  VAL C  41
SHEET    3   D 8 THR C  82  PRO C  86 -1  O  VAL C  83   N  GLY C  51
SHEET    4   D 8 LEU C  56  VAL C  60  1  N  VAL C  57   O  THR C  82
SHEET    5   D 8 PHE C 123  HIS C 128  1  O  VAL C 126   N  VAL C  60
SHEET    6   D 8 ILE C 146  MET C 152  1  O  VAL C 150   N  LEU C 125
SHEET    7   D 8 THR C 261  GLY C 266  1  O  MET C 262   N  TYR C 151
SHEET    8   D 8 VAL C 287  LEU C 292  1  O  LEU C 292   N  ALA C 265
SHEET    1   E 8 GLU D  35  VAL D  41  0
SHEET    2   E 8 VAL D  44  GLY D  52 -1  O  LEU D  46   N  ARG D  39
SHEET    3   E 8 THR D  82  PRO D  86 -1  O  VAL D  83   N  GLY D  51
SHEET    4   E 8 LEU D  56  VAL D  60  1  N  VAL D  57   O  THR D  82
SHEET    5   E 8 PHE D 123  HIS D 128  1  O  VAL D 126   N  VAL D  60
SHEET    6   E 8 ILE D 146  MET D 152  1  O  VAL D 150   N  LEU D 125
SHEET    7   E 8 THR D 261  GLY D 266  1  O  MET D 262   N  LEU D 149
SHEET    8   E 8 VAL D 287  LEU D 292  1  O  LEU D 292   N  ALA D 265
SHEET    1   F 2 PHE D 167  THR D 168  0
SHEET    2   F 2 GLY D 171  GLU D 172 -1  O  GLY D 171   N  THR D 168
SSBOND   1 CYS A  295    CYS A  303                          1555   1555  2.02
SSBOND   2 CYS B  295    CYS B  303                          1555   1555  2.02
SSBOND   3 CYS C  295    CYS C  303                          1555   1555  2.02
SSBOND   4 CYS D  295    CYS D  303                          1555   1555  2.01
SITE     1 AC1 10 ASP A 129  GLU A 153  ALA A 154  PRO A 155
SITE     2 AC1 10 HIS A 177  PHE A 178  TYR A 239  HIS A 297
SITE     3 AC1 10 HOH A 525  HOH A 695
SITE     1 AC2  7 ASP B 129  ALA B 154  PRO B 155  HIS B 177
SITE     2 AC2  7 PHE B 178  TYR B 239  HOH B 528
SITE     1 AC3  6 ASP C 129  ALA C 154  HIS C 177  TYR C 239
SITE     2 AC3  6 HOH C 524  HOH C 701
SITE     1 AC4  7 ASP D 129  ALA D 154  PRO D 155  HIS D 177
SITE     2 AC4  7 PHE D 178  TYR D 239  HOH D 514
CRYST1  168.839   83.761   89.524  90.00 100.48  90.00 C 1 2 1      16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.005923  0.000000  0.001096        0.00000
SCALE2      0.000000  0.011939  0.000000        0.00000
SCALE3      0.000000  0.000000  0.011360        0.00000
TER    2378      HIS A 321
TER    4746      HIS B 320
TER    7117      HIS C 320
TER    9495      HIS D 321
MASTER      401    0    4   64   36    0    9    610300    4   32   96
END