longtext: 4J0I-pdb

content
HEADER    HYDROLASE                               30-JAN-13   4J0I
TITLE     TANNIN ACYL HYDROLASE IN COMPLEX WITH 3,4-DIHYDROXYBENZOATE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: TANNASE;
COMPND   3 CHAIN: A, B;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: LACTOBACILLUS PLANTARUM;
SOURCE   3 ORGANISM_TAXID: 1590;
SOURCE   4 GENE: TANLPL;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE   7 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID
KEYWDS    TANNIN, HYDROLASE, HYDROLYSIS
EXPDTA    X-RAY DIFFRACTION
AUTHOR    B.REN,M.WU,Q.WANG,X.PENG,H.WEN,Q.CHEN,W.J.MCKINSTRY
REVDAT   1   22-MAY-13 4J0I    0
JRNL        AUTH   B.REN,M.WU,Q.WANG,X.PENG,H.WEN,W.J.MCKINSTRY,Q.CHEN
JRNL        TITL   CRYSTAL STRUCTURE OF TANNASE FROM LACTOBACILLUS PLANTARUM
JRNL        REF    J.MOL.BIOL.                                2013
JRNL        REFN                   ESSN 1089-8638
JRNL        PMID   23648840
JRNL        DOI    10.1016/J.JMB.2013.04.032
REMARK   2
REMARK   2 RESOLUTION.    1.75 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.7.1_743)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER
REMARK   3               : ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.75
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.74
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.990
REMARK   3   COMPLETENESS FOR RANGE        (%) : 96.9
REMARK   3   NUMBER OF REFLECTIONS             : 86020
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.156
REMARK   3   R VALUE            (WORKING SET) : 0.155
REMARK   3   FREE R VALUE                     : 0.188
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.020
REMARK   3   FREE R VALUE TEST SET COUNT      : 4315
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 19.7425 -  5.4016    0.98     2742   153  0.1937 0.2167
REMARK   3     2  5.4016 -  4.3019    0.97     2687   170  0.1367 0.1446
REMARK   3     3  4.3019 -  3.7624    0.96     2709   146  0.1339 0.1608
REMARK   3     4  3.7624 -  3.4204    0.96     2686   147  0.1357 0.1386
REMARK   3     5  3.4204 -  3.1763    0.96     2685   141  0.1472 0.1693
REMARK   3     6  3.1763 -  2.9897    0.97     2702   146  0.1528 0.1839
REMARK   3     7  2.9897 -  2.8404    0.97     2758   133  0.1546 0.1750
REMARK   3     8  2.8404 -  2.7171    0.98     2739   140  0.1549 0.2032
REMARK   3     9  2.7171 -  2.6127    0.98     2724   151  0.1462 0.1785
REMARK   3    10  2.6127 -  2.5228    0.98     2760   148  0.1502 0.1982
REMARK   3    11  2.5228 -  2.4440    0.97     2705   157  0.1437 0.1973
REMARK   3    12  2.4440 -  2.3743    0.97     2785   134  0.1343 0.1765
REMARK   3    13  2.3743 -  2.3119    0.97     2715   147  0.1384 0.1791
REMARK   3    14  2.3119 -  2.2556    0.97     2748   147  0.1391 0.1929
REMARK   3    15  2.2556 -  2.2044    0.97     2732   135  0.1422 0.1851
REMARK   3    16  2.2044 -  2.1575    0.98     2715   148  0.1396 0.1770
REMARK   3    17  2.1575 -  2.1144    0.97     2719   147  0.1376 0.1946
REMARK   3    18  2.1144 -  2.0745    0.97     2809   139  0.1467 0.1581
REMARK   3    19  2.0745 -  2.0375    0.97     2701   136  0.1394 0.1885
REMARK   3    20  2.0375 -  2.0030    0.97     2743   147  0.1522 0.1843
REMARK   3    21  2.0030 -  1.9707    0.97     2693   144  0.1563 0.2221
REMARK   3    22  1.9707 -  1.9404    0.97     2697   154  0.1631 0.2300
REMARK   3    23  1.9404 -  1.9119    0.97     2760   146  0.1686 0.2181
REMARK   3    24  1.9119 -  1.8850    0.97     2720   150  0.1770 0.2275
REMARK   3    25  1.8850 -  1.8596    0.97     2668   127  0.1834 0.2345
REMARK   3    26  1.8596 -  1.8354    0.96     2752   140  0.2014 0.2499
REMARK   3    27  1.8354 -  1.8125    0.96     2725   148  0.2100 0.2678
REMARK   3    28  1.8125 -  1.7907    0.96     2650   125  0.2294 0.2847
REMARK   3    29  1.7907 -  1.7699    0.96     2775   141  0.2321 0.2715
REMARK   3    30  1.7699 -  1.7500    0.96     2701   128  0.2402 0.2764
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.10
REMARK   3   SHRINKAGE RADIUS   : 0.83
REMARK   3   K_SOL              : 0.41
REMARK   3   B_SOL              : 57.89
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.400
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 18.450
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 20.33
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 5.32280
REMARK   3    B22 (A**2) : -6.24560
REMARK   3    B33 (A**2) : 0.92280
REMARK   3    B12 (A**2) : -2.57660
REMARK   3    B13 (A**2) : 4.73530
REMARK   3    B23 (A**2) : -0.34260
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.008           7507
REMARK   3   ANGLE     :  1.081          10270
REMARK   3   CHIRALITY :  0.072           1153
REMARK   3   PLANARITY :  0.004           1352
REMARK   3   DIHEDRAL  : 13.835           2706
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 6
REMARK   3   TLS GROUP : 1
REMARK   3    SELECTION: chain 'A' and (resseq 1:228)
REMARK   3    ORIGIN FOR THE GROUP (A):  27.3119 105.0215  80.4441
REMARK   3    T TENSOR
REMARK   3      T11:   0.1266 T22:   0.1056
REMARK   3      T33:   0.0928 T12:   0.0125
REMARK   3      T13:  -0.0120 T23:   0.0073
REMARK   3    L TENSOR
REMARK   3      L11:   0.1819 L22:   1.2719
REMARK   3      L33:   0.8200 L12:   0.0442
REMARK   3      L13:   0.0139 L23:   0.2201
REMARK   3    S TENSOR
REMARK   3      S11:   0.0126 S12:   0.0079 S13:   0.0361
REMARK   3      S21:   0.1400 S22:  -0.0032 S23:  -0.0392
REMARK   3      S31:  -0.1505 S32:  -0.0117 S33:  -0.0039
REMARK   3   TLS GROUP : 2
REMARK   3    SELECTION: chain 'A' and (resseq 247:340)
REMARK   3    ORIGIN FOR THE GROUP (A):  20.9393  74.2810  71.1974
REMARK   3    T TENSOR
REMARK   3      T11:   0.1893 T22:   0.1166
REMARK   3      T33:   0.1138 T12:   0.0029
REMARK   3      T13:   0.0001 T23:  -0.0022
REMARK   3    L TENSOR
REMARK   3      L11:   0.5650 L22:   0.9391
REMARK   3      L33:   1.7716 L12:   0.2546
REMARK   3      L13:  -0.4513 L23:  -0.2997
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0782 S12:   0.0160 S13:  -0.0217
REMARK   3      S21:  -0.0385 S22:   0.0396 S23:   0.0406
REMARK   3      S31:   0.2892 S32:  -0.0677 S33:   0.0422
REMARK   3   TLS GROUP : 3
REMARK   3    SELECTION: chain 'A' and (resseq 341:469)
REMARK   3    ORIGIN FOR THE GROUP (A):  25.9730  91.2379  70.2158
REMARK   3    T TENSOR
REMARK   3      T11:   0.0908 T22:   0.1167
REMARK   3      T33:   0.1073 T12:   0.0194
REMARK   3      T13:  -0.0058 T23:  -0.0072
REMARK   3    L TENSOR
REMARK   3      L11:   0.4078 L22:   0.9869
REMARK   3      L33:   1.1178 L12:   0.2317
REMARK   3      L13:   0.0885 L23:   0.1056
REMARK   3    S TENSOR
REMARK   3      S11:   0.0080 S12:   0.0383 S13:   0.0011
REMARK   3      S21:   0.0450 S22:   0.0344 S23:  -0.0828
REMARK   3      S31:   0.0185 S32:   0.0602 S33:  -0.0381
REMARK   3   TLS GROUP : 4
REMARK   3    SELECTION: chain 'B' and (resseq 1:209)
REMARK   3    ORIGIN FOR THE GROUP (A):  39.6268  41.8716  33.6487
REMARK   3    T TENSOR
REMARK   3      T11:   0.0627 T22:   0.1004
REMARK   3      T33:   0.1072 T12:   0.0037
REMARK   3      T13:   0.0020 T23:  -0.0010
REMARK   3    L TENSOR
REMARK   3      L11:   0.4260 L22:   1.1477
REMARK   3      L33:   0.5280 L12:  -0.0589
REMARK   3      L13:  -0.1828 L23:   0.2650
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0053 S12:   0.0313 S13:  -0.0609
REMARK   3      S21:  -0.0754 S22:  -0.0058 S23:  -0.0419
REMARK   3      S31:   0.0294 S32:  -0.0059 S33:   0.0218
REMARK   3   TLS GROUP : 5
REMARK   3    SELECTION: chain 'B' and (resseq 210:311)
REMARK   3    ORIGIN FOR THE GROUP (A):  30.6624  71.7940  40.0784
REMARK   3    T TENSOR
REMARK   3      T11:   0.1211 T22:   0.0979
REMARK   3      T33:   0.0972 T12:  -0.0076
REMARK   3      T13:  -0.0073 T23:  -0.0146
REMARK   3    L TENSOR
REMARK   3      L11:   1.2889 L22:   1.3192
REMARK   3      L33:   0.8589 L12:  -0.1212
REMARK   3      L13:  -0.2678 L23:   0.1063
REMARK   3    S TENSOR
REMARK   3      S11:   0.0125 S12:   0.0404 S13:   0.0256
REMARK   3      S21:  -0.2163 S22:   0.0159 S23:   0.0396
REMARK   3      S31:  -0.0698 S32:  -0.0846 S33:  -0.0403
REMARK   3   TLS GROUP : 6
REMARK   3    SELECTION: chain 'B' and (resseq 312:469)
REMARK   3    ORIGIN FOR THE GROUP (A):  34.7368  63.7449  39.9373
REMARK   3    T TENSOR
REMARK   3      T11:   0.0733 T22:   0.1059
REMARK   3      T33:   0.0816 T12:  -0.0050
REMARK   3      T13:  -0.0049 T23:  -0.0061
REMARK   3    L TENSOR
REMARK   3      L11:   0.4894 L22:   0.8905
REMARK   3      L33:   0.2937 L12:  -0.0656
REMARK   3      L13:  -0.0269 L23:   0.1077
REMARK   3    S TENSOR
REMARK   3      S11:   0.0090 S12:  -0.0315 S13:  -0.0015
REMARK   3      S21:  -0.0770 S22:   0.0064 S23:  -0.0329
REMARK   3      S31:  -0.0393 S32:   0.0227 S33:  -0.0116
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 4J0I COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 13-FEB-13.
REMARK 100 THE RCSB ID CODE IS RCSB077441.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : NULL
REMARK 200  TEMPERATURE           (KELVIN) : NULL
REMARK 200  PH                             : 5.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : AUSTRALIAN SYNCHROTRON
REMARK 200  BEAMLINE                       : MX2
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : NULL
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 328191
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.750
REMARK 200  RESOLUTION RANGE LOW       (A) : NULL
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 96.8
REMARK 200  DATA REDUNDANCY                : 3.800
REMARK 200  R MERGE                    (I) : 0.08800
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 10.7000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.75
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.84
REMARK 200  COMPLETENESS FOR SHELL     (%) : 96.2
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.90
REMARK 200  R MERGE FOR SHELL          (I) : 0.53000
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 2.500
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: FOURIER SYNTHESIS
REMARK 200 SOFTWARE USED: PHENIX
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 42.32
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.13
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M TRI-SODIUM CITRATE PH 5.5, 20%
REMARK 280  PEG 3000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 281K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A   -21
REMARK 465     HIS A   -20
REMARK 465     HIS A   -19
REMARK 465     HIS A   -18
REMARK 465     HIS A   -17
REMARK 465     HIS A   -16
REMARK 465     HIS A   -15
REMARK 465     SER A   -14
REMARK 465     SER A   -13
REMARK 465     GLY A   -12
REMARK 465     VAL A   -11
REMARK 465     ASP A   -10
REMARK 465     LEU A    -9
REMARK 465     GLY A    -8
REMARK 465     THR A    -7
REMARK 465     GLU A    -6
REMARK 465     ASN A    -5
REMARK 465     LEU A    -4
REMARK 465     TYR A    -3
REMARK 465     PHE A    -2
REMARK 465     GLN A    -1
REMARK 465     TYR A   229
REMARK 465     GLN A   230
REMARK 465     PRO A   231
REMARK 465     VAL A   232
REMARK 465     ALA A   233
REMARK 465     GLY A   234
REMARK 465     THR A   235
REMARK 465     THR A   236
REMARK 465     LYS A   237
REMARK 465     ASN A   238
REMARK 465     GLY A   239
REMARK 465     ARG A   240
REMARK 465     PRO A   241
REMARK 465     LYS A   242
REMARK 465     PHE A   243
REMARK 465     GLU A   244
REMARK 465     PRO A   245
REMARK 465     VAL A   246
REMARK 465     MET B   -21
REMARK 465     HIS B   -20
REMARK 465     HIS B   -19
REMARK 465     HIS B   -18
REMARK 465     HIS B   -17
REMARK 465     HIS B   -16
REMARK 465     HIS B   -15
REMARK 465     SER B   -14
REMARK 465     SER B   -13
REMARK 465     GLY B   -12
REMARK 465     VAL B   -11
REMARK 465     ASP B   -10
REMARK 465     LEU B    -9
REMARK 465     GLY B    -8
REMARK 465     THR B    -7
REMARK 465     GLU B    -6
REMARK 465     ASN B    -5
REMARK 465     LEU B    -4
REMARK 465     TYR B    -3
REMARK 465     PHE B    -2
REMARK 465     GLN B    -1
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     LYS B 237    CG   CD   CE   NZ
REMARK 470     ARG B 240    CG   CD   NE   CZ   NH1  NH2
REMARK 480
REMARK 480 ZERO OCCUPANCY ATOM
REMARK 480 THE FOLLOWING RESIDUES HAVE ATOMS MODELED WITH ZERO
REMARK 480 OCCUPANCY. THE LOCATION AND PROPERTIES OF THESE ATOMS
REMARK 480 MAY NOT BE RELIABLE. (M=MODEL NUMBER; RES=RESIDUE NAME;
REMARK 480 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 480   M RES C SSEQI ATOMS
REMARK 480     ILE B  140   CA   CB   CG1  CG2  CD1
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    HOH B   747     O    HOH B   830              1.81
REMARK 500   O    HOH B   878     O    HOH B   888              1.82
REMARK 500   OE1  GLN B    22     O    HOH B   825              1.85
REMARK 500   O    HOH B   905     O    HOH B   970              1.85
REMARK 500   O    HOH B   973     O    HOH B   980              1.85
REMARK 500   O    HOH B   979     O    HOH B   987              1.87
REMARK 500   O    HOH B   961     O    HOH B  1005              1.87
REMARK 500   O    HOH B   937     O    HOH B   992              1.88
REMARK 500   O    HOH B   912     O    HOH B   953              1.89
REMARK 500   O    HOH A   735     O    HOH A   794              1.89
REMARK 500   O    HOH B   977     O    HOH B   988              1.90
REMARK 500   O    HOH B   789     O    HOH B   827              1.91
REMARK 500   O    HOH A   794     O    HOH A   818              1.92
REMARK 500   O    HOH B   964     O    HOH B   992              1.92
REMARK 500   O    HOH A   866     O    HOH A   870              1.93
REMARK 500   O    HOH A   682     O    HOH A   688              1.94
REMARK 500   O    HOH A   958     O    HOH A   965              1.94
REMARK 500   O    HOH A   912     O    HOH A   942              1.94
REMARK 500   O    HOH A   757     O    HOH A   807              1.95
REMARK 500   O    HOH B   901     O    HOH B   926              1.96
REMARK 500   O    HOH B   882     O    HOH B   904              1.96
REMARK 500   O    HOH B   978     O    HOH B   982              1.96
REMARK 500   O    HOH A   722     O    HOH A   962              1.97
REMARK 500   O    HOH B   906     O    HOH B   922              1.99
REMARK 500   O    HOH A   839     O    HOH A   948              2.00
REMARK 500   O    HOH B   972     O    HOH B   985              2.00
REMARK 500   OG1  THR B   267     O    HOH B   974              2.00
REMARK 500   O    HOH A   947     O    HOH A   964              2.01
REMARK 500   OE1  GLN A   350     OG1  THR A   354              2.02
REMARK 500   OE1  GLN A   124     O    HOH A   941              2.02
REMARK 500   NZ   LYS B   103     O    HOH B   897              2.03
REMARK 500   O    HOH B   963     O    HOH B   993              2.05
REMARK 500   O    HOH B   814     O    HOH B   882              2.05
REMARK 500   O    HOH B   951     O    HOH B   984              2.06
REMARK 500   OD1  ASP A   294     NH2  ARG A   297              2.07
REMARK 500   OE1  GLN B   350     OG1  THR B   354              2.07
REMARK 500   NH2  ARG B    64     O    HOH B   966              2.07
REMARK 500   O1   PG4 B   502     O    HOH B   987              2.08
REMARK 500   O    HOH A   855     O    HOH A   859              2.12
REMARK 500   O    HOH A   750     O    HOH A   888              2.13
REMARK 500   O    HOH A   641     O    HOH A   936              2.13
REMARK 500   O    HOH A   921     O    HOH A   937              2.14
REMARK 500   O    HOH B   876     O    HOH B   927              2.15
REMARK 500   O1   PEG B   506     O    HOH B   921              2.15
REMARK 500   O    HOH A   910     O    HOH A   928              2.15
REMARK 500   O    HOH A   733     O    HOH A   827              2.15
REMARK 500   O    HOH B   741     O    HOH B   932              2.17
REMARK 500   OE1  GLN A   350     O    HOH A   895              2.17
REMARK 500   OE1  GLN B   350     O    HOH B   917              2.19
REMARK 500   O    HOH A   790     O    HOH A   814              2.19
REMARK 500
REMARK 500 THIS ENTRY HAS      51 CLOSE CONTACTS
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   O    HOH B   986     O    HOH B   998     1545     2.13
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A 163     -121.60     64.74
REMARK 500    CYS A 204       58.04     31.20
REMARK 500    THR A 340      145.62     79.77
REMARK 500    ALA A 344     -158.63    -83.04
REMARK 500    PHE A 371       15.51   -145.45
REMARK 500    THR A 403       46.54   -109.50
REMARK 500    PRO B 130       33.24    -98.45
REMARK 500    SER B 163     -121.58     66.92
REMARK 500    CYS B 204       56.18     35.56
REMARK 500    ARG B 228     -156.11   -150.41
REMARK 500    LYS B 237      -37.64    -35.73
REMARK 500    THR B 340      152.80     78.59
REMARK 500    ALA B 344     -156.98    -85.90
REMARK 500    PHE B 348      -53.36   -121.08
REMARK 500    PHE B 371       16.76   -144.36
REMARK 500    HIS B 451       99.64    -69.10
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A 952        DISTANCE =  5.26 ANGSTROMS
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE DHB A 501
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG A 502
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG A 503
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE DHB B 501
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PG4 B 502
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B 503
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B 504
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B 505
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B 506
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PEG B 507
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4J0C   RELATED DB: PDB
REMARK 900 RELATED ID: 4J0D   RELATED DB: PDB
REMARK 900 RELATED ID: 4J0G   RELATED DB: PDB
REMARK 900 RELATED ID: 4J0H   RELATED DB: PDB
REMARK 900 RELATED ID: 4J0J   RELATED DB: PDB
REMARK 900 RELATED ID: 4J0K   RELATED DB: PDB
REMARK 900 RELATED ID: 4JUI   RELATED DB: PDB
DBREF  4J0I A    1   469  UNP    B3Y018   B3Y018_LACPN     1    469
DBREF  4J0I B    1   469  UNP    B3Y018   B3Y018_LACPN     1    469
SEQADV 4J0I MET A  -21  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS A  -20  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS A  -19  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS A  -18  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS A  -17  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS A  -16  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS A  -15  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I SER A  -14  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I SER A  -13  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I GLY A  -12  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I VAL A  -11  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I ASP A  -10  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I LEU A   -9  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I GLY A   -8  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I THR A   -7  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I GLU A   -6  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I ASN A   -5  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I LEU A   -4  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I TYR A   -3  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I PHE A   -2  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I GLN A   -1  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I SER A    0  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I MET B  -21  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS B  -20  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS B  -19  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS B  -18  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS B  -17  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS B  -16  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I HIS B  -15  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I SER B  -14  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I SER B  -13  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I GLY B  -12  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I VAL B  -11  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I ASP B  -10  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I LEU B   -9  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I GLY B   -8  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I THR B   -7  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I GLU B   -6  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I ASN B   -5  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I LEU B   -4  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I TYR B   -3  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I PHE B   -2  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I GLN B   -1  UNP  B3Y018              EXPRESSION TAG
SEQADV 4J0I SER B    0  UNP  B3Y018              EXPRESSION TAG
SEQRES   1 A  491  MET HIS HIS HIS HIS HIS HIS SER SER GLY VAL ASP LEU
SEQRES   2 A  491  GLY THR GLU ASN LEU TYR PHE GLN SER MET SER ASN ARG
SEQRES   3 A  491  LEU ILE PHE ASP ALA ASP TRP LEU VAL PRO GLU GLN VAL
SEQRES   4 A  491  GLN VAL ALA GLY GLN ALA ILE GLN TYR TYR ALA ALA ARG
SEQRES   5 A  491  ASN ILE GLN TYR VAL GLN HIS PRO VAL ALA ALA ILE GLN
SEQRES   6 A  491  VAL LEU ASN VAL PHE VAL PRO ALA ALA TYR LEU HIS GLY
SEQRES   7 A  491  SER SER VAL ASN GLY TYR GLN ARG ALA THR ALA PRO ILE
SEQRES   8 A  491  LEU MET PRO ASN THR VAL GLY GLY TYR LEU PRO GLY PRO
SEQRES   9 A  491  ALA ASP ASP PRO GLN ARG VAL THR TRP PRO THR ASN ALA
SEQRES  10 A  491  GLY THR ILE GLN GLN ALA LEU LYS ARG GLY TYR VAL VAL
SEQRES  11 A  491  VAL ALA ALA GLY ILE ARG GLY ARG THR THR VAL ASP LYS
SEQRES  12 A  491  SER GLY GLN ARG VAL GLY GLN ALA PRO ALA PHE ILE VAL
SEQRES  13 A  491  ASP MET LYS ALA ALA ILE ARG TYR VAL LYS TYR ASN GLN
SEQRES  14 A  491  GLY ARG LEU PRO GLY ASP ALA ASN ARG ILE ILE THR ASN
SEQRES  15 A  491  GLY THR SER ALA GLY GLY ALA THR SER ALA LEU ALA GLY
SEQRES  16 A  491  ALA SER GLY ASN SER ALA TYR PHE GLU PRO ALA LEU THR
SEQRES  17 A  491  ALA LEU GLY ALA ALA PRO ALA THR ASP ASP ILE PHE ALA
SEQRES  18 A  491  VAL SER ALA TYR CYS PRO ILE HIS ASN LEU GLU HIS ALA
SEQRES  19 A  491  ASP MET ALA TYR GLU TRP GLN PHE ASN GLY ILE ASN ASP
SEQRES  20 A  491  TRP HIS ARG TYR GLN PRO VAL ALA GLY THR THR LYS ASN
SEQRES  21 A  491  GLY ARG PRO LYS PHE GLU PRO VAL SER GLY GLN LEU THR
SEQRES  22 A  491  VAL GLU GLU GLN ALA LEU SER LEU ALA LEU LYS ALA GLN
SEQRES  23 A  491  PHE SER THR TYR LEU ASN GLN LEU LYS LEU THR ALA SER
SEQRES  24 A  491  ASP GLY THR HIS LEU THR LEU ASN GLU ALA GLY MET GLY
SEQRES  25 A  491  SER PHE ARG ASP VAL VAL ARG GLN LEU LEU ILE SER SER
SEQRES  26 A  491  ALA GLN THR ALA PHE ASP GLN GLY THR ASP ILE HIS LYS
SEQRES  27 A  491  TYR ALA GLY PHE VAL VAL THR GLY ASN GLN VAL THR ASP
SEQRES  28 A  491  LEU ASP LEU SER ALA TYR LEU LYS SER LEU THR ARG MET
SEQRES  29 A  491  LYS ALA VAL PRO ALA PHE ASP GLN LEU ASP LEU THR SER
SEQRES  30 A  491  PRO GLU ASN ASN LEU PHE GLY ASP ALA THR ALA LYS ALA
SEQRES  31 A  491  LYS HIS PHE THR ALA LEU ALA GLN THR ARG SER THR VAL
SEQRES  32 A  491  THR ALA GLN LEU ALA ASP ALA GLU LEU ILE GLN ALA ILE
SEQRES  33 A  491  ASN PRO LEU SER TYR LEU THR THR THR SER SER GLN VAL
SEQRES  34 A  491  ALA LYS HIS TRP ARG ILE ARG HIS GLY ALA ALA ASP ARG
SEQRES  35 A  491  ASP THR SER PHE ALA ILE PRO ILE ILE LEU ALA ILE MET
SEQRES  36 A  491  LEU GLU ASN HIS GLY TYR GLY ILE ASP PHE ALA LEU PRO
SEQRES  37 A  491  TRP ASP ILE PRO HIS SER GLY ASP TYR ASP LEU GLY ASP
SEQRES  38 A  491  LEU PHE SER TRP ILE ASP GLY LEU CYS GLN
SEQRES   1 B  491  MET HIS HIS HIS HIS HIS HIS SER SER GLY VAL ASP LEU
SEQRES   2 B  491  GLY THR GLU ASN LEU TYR PHE GLN SER MET SER ASN ARG
SEQRES   3 B  491  LEU ILE PHE ASP ALA ASP TRP LEU VAL PRO GLU GLN VAL
SEQRES   4 B  491  GLN VAL ALA GLY GLN ALA ILE GLN TYR TYR ALA ALA ARG
SEQRES   5 B  491  ASN ILE GLN TYR VAL GLN HIS PRO VAL ALA ALA ILE GLN
SEQRES   6 B  491  VAL LEU ASN VAL PHE VAL PRO ALA ALA TYR LEU HIS GLY
SEQRES   7 B  491  SER SER VAL ASN GLY TYR GLN ARG ALA THR ALA PRO ILE
SEQRES   8 B  491  LEU MET PRO ASN THR VAL GLY GLY TYR LEU PRO GLY PRO
SEQRES   9 B  491  ALA ASP ASP PRO GLN ARG VAL THR TRP PRO THR ASN ALA
SEQRES  10 B  491  GLY THR ILE GLN GLN ALA LEU LYS ARG GLY TYR VAL VAL
SEQRES  11 B  491  VAL ALA ALA GLY ILE ARG GLY ARG THR THR VAL ASP LYS
SEQRES  12 B  491  SER GLY GLN ARG VAL GLY GLN ALA PRO ALA PHE ILE VAL
SEQRES  13 B  491  ASP MET LYS ALA ALA ILE ARG TYR VAL LYS TYR ASN GLN
SEQRES  14 B  491  GLY ARG LEU PRO GLY ASP ALA ASN ARG ILE ILE THR ASN
SEQRES  15 B  491  GLY THR SER ALA GLY GLY ALA THR SER ALA LEU ALA GLY
SEQRES  16 B  491  ALA SER GLY ASN SER ALA TYR PHE GLU PRO ALA LEU THR
SEQRES  17 B  491  ALA LEU GLY ALA ALA PRO ALA THR ASP ASP ILE PHE ALA
SEQRES  18 B  491  VAL SER ALA TYR CYS PRO ILE HIS ASN LEU GLU HIS ALA
SEQRES  19 B  491  ASP MET ALA TYR GLU TRP GLN PHE ASN GLY ILE ASN ASP
SEQRES  20 B  491  TRP HIS ARG TYR GLN PRO VAL ALA GLY THR THR LYS ASN
SEQRES  21 B  491  GLY ARG PRO LYS PHE GLU PRO VAL SER GLY GLN LEU THR
SEQRES  22 B  491  VAL GLU GLU GLN ALA LEU SER LEU ALA LEU LYS ALA GLN
SEQRES  23 B  491  PHE SER THR TYR LEU ASN GLN LEU LYS LEU THR ALA SER
SEQRES  24 B  491  ASP GLY THR HIS LEU THR LEU ASN GLU ALA GLY MET GLY
SEQRES  25 B  491  SER PHE ARG ASP VAL VAL ARG GLN LEU LEU ILE SER SER
SEQRES  26 B  491  ALA GLN THR ALA PHE ASP GLN GLY THR ASP ILE HIS LYS
SEQRES  27 B  491  TYR ALA GLY PHE VAL VAL THR GLY ASN GLN VAL THR ASP
SEQRES  28 B  491  LEU ASP LEU SER ALA TYR LEU LYS SER LEU THR ARG MET
SEQRES  29 B  491  LYS ALA VAL PRO ALA PHE ASP GLN LEU ASP LEU THR SER
SEQRES  30 B  491  PRO GLU ASN ASN LEU PHE GLY ASP ALA THR ALA LYS ALA
SEQRES  31 B  491  LYS HIS PHE THR ALA LEU ALA GLN THR ARG SER THR VAL
SEQRES  32 B  491  THR ALA GLN LEU ALA ASP ALA GLU LEU ILE GLN ALA ILE
SEQRES  33 B  491  ASN PRO LEU SER TYR LEU THR THR THR SER SER GLN VAL
SEQRES  34 B  491  ALA LYS HIS TRP ARG ILE ARG HIS GLY ALA ALA ASP ARG
SEQRES  35 B  491  ASP THR SER PHE ALA ILE PRO ILE ILE LEU ALA ILE MET
SEQRES  36 B  491  LEU GLU ASN HIS GLY TYR GLY ILE ASP PHE ALA LEU PRO
SEQRES  37 B  491  TRP ASP ILE PRO HIS SER GLY ASP TYR ASP LEU GLY ASP
SEQRES  38 B  491  LEU PHE SER TRP ILE ASP GLY LEU CYS GLN
HET    DHB  A 501      11
HET    PEG  A 502       7
HET    PEG  A 503       7
HET    DHB  B 501      11
HET    PG4  B 502      13
HET    PEG  B 503       7
HET    PEG  B 504       7
HET    PEG  B 505       7
HET    PEG  B 506       7
HET    PEG  B 507       7
HETNAM     DHB 3,4-DIHYDROXYBENZOIC ACID
HETNAM     PEG DI(HYDROXYETHYL)ETHER
HETNAM     PG4 TETRAETHYLENE GLYCOL
FORMUL   3  DHB    2(C7 H6 O4)
FORMUL   4  PEG    7(C4 H10 O3)
FORMUL   7  PG4    C8 H18 O5
FORMUL  13  HOH   *775(H2 O)
HELIX    1   1 ASP A    8  LEU A   12  5                                   5
HELIX    2   2 ALA A   52  HIS A   55  5                                   4
HELIX    3   3 ASN A   94  GLY A  105  1                                  12
HELIX    4   4 PRO A  130  ASN A  146  1                                  17
HELIX    5   5 ASP A  153  ASN A  155  5                                   3
HELIX    6   6 SER A  163  SER A  175  1                                  13
HELIX    7   7 SER A  178  TYR A  180  5                                   3
HELIX    8   8 PHE A  181  GLY A  189  1                                   9
HELIX    9   9 ASN A  208  ASN A  221  1                                  14
HELIX   10  10 THR A  251  LEU A  272  1                                  22
HELIX   11  11 GLY A  290  GLN A  310  1                                  21
HELIX   12  12 ASP A  313  TYR A  317  5                                   5
HELIX   13  13 ASP A  331  THR A  340  1                                  10
HELIX   14  14 SER A  355  PHE A  361  1                                   7
HELIX   15  15 THR A  372  ARG A  378  1                                   7
HELIX   16  16 ASP A  387  ILE A  394  1                                   8
HELIX   17  17 ASN A  395  LEU A  400  5                                   6
HELIX   18  18 PHE A  424  HIS A  437  1                                  14
HELIX   19  19 ASP A  456  GLN A  469  1                                  14
HELIX   20  20 ASP B    8  LEU B   12  5                                   5
HELIX   21  21 ALA B   52  HIS B   55  5                                   4
HELIX   22  22 ASN B   94  GLY B  105  1                                  12
HELIX   23  23 PRO B  130  ASN B  146  1                                  17
HELIX   24  24 ASP B  153  ASN B  155  5                                   3
HELIX   25  25 SER B  163  SER B  175  1                                  13
HELIX   26  26 SER B  178  TYR B  180  5                                   3
HELIX   27  27 PHE B  181  GLY B  189  1                                   9
HELIX   28  28 ASN B  208  ASN B  221  1                                  14
HELIX   29  29 THR B  251  LEU B  272  1                                  22
HELIX   30  30 GLY B  290  ASP B  309  1                                  20
HELIX   31  31 ASP B  313  TYR B  317  5                                   5
HELIX   32  32 ASP B  331  THR B  340  1                                  10
HELIX   33  33 SER B  355  PHE B  361  1                                   7
HELIX   34  34 THR B  372  ARG B  378  1                                   7
HELIX   35  35 ASP B  387  ILE B  394  1                                   8
HELIX   36  36 ASN B  395  LEU B  400  5                                   6
HELIX   37  37 PHE B  424  HIS B  437  1                                  14
HELIX   38  38 ASP B  456  GLN B  469  1                                  14
SHEET    1   A 9 VAL A  13  VAL A  19  0
SHEET    2   A 9 GLN A  22  GLN A  33 -1  O  GLN A  22   N  VAL A  19
SHEET    3   A 9 VAL A  44  PRO A  50 -1  O  VAL A  47   N  ALA A  29
SHEET    4   A 9 VAL A 107  ALA A 111 -1  O  VAL A 108   N  PHE A  48
SHEET    5   A 9 ILE A  69  PRO A  72  1  N  LEU A  70   O  VAL A 107
SHEET    6   A 9 ILE A 157  THR A 162  1  O  ILE A 158   N  MET A  71
SHEET    7   A 9 ALA A 199  TYR A 203  1  O  TYR A 203   N  GLY A 161
SHEET    8   A 9 HIS A 410  ARG A 414  1  O  HIS A 410   N  VAL A 200
SHEET    9   A 9 GLY A 440  ALA A 444  1  O  ASP A 442   N  ILE A 413
SHEET    1   B 2 ASP A 225  TRP A 226  0
SHEET    2   B 2 GLY A 248  GLN A 249 -1  O  GLY A 248   N  TRP A 226
SHEET    1   C 2 PHE A 320  THR A 323  0
SHEET    2   C 2 GLN A 326  LEU A 330 -1  O  GLN A 326   N  THR A 323
SHEET    1   D 9 VAL B  13  VAL B  19  0
SHEET    2   D 9 GLN B  22  GLN B  33 -1  O  TYR B  26   N  GLU B  15
SHEET    3   D 9 VAL B  44  PRO B  50 -1  O  LEU B  45   N  ILE B  32
SHEET    4   D 9 VAL B 107  ALA B 111 -1  O  VAL B 108   N  PHE B  48
SHEET    5   D 9 ILE B  69  PRO B  72  1  N  LEU B  70   O  VAL B 107
SHEET    6   D 9 ILE B 157  THR B 162  1  O  ILE B 158   N  MET B  71
SHEET    7   D 9 ALA B 199  TYR B 203  1  O  TYR B 203   N  GLY B 161
SHEET    8   D 9 HIS B 410  ARG B 414  1  O  ARG B 412   N  VAL B 200
SHEET    9   D 9 GLY B 440  ALA B 444  1  O  ASP B 442   N  ILE B 413
SHEET    1   E 2 ASP B 225  PRO B 231  0
SHEET    2   E 2 PHE B 243  GLN B 249 -1  O  VAL B 246   N  ARG B 228
SHEET    1   F 2 PHE B 320  THR B 323  0
SHEET    2   F 2 GLN B 326  LEU B 330 -1  O  GLN B 326   N  THR B 323
CISPEP   1 TRP A   91    PRO A   92          0        -1.72
CISPEP   2 ALA A  129    PRO A  130          0         4.64
CISPEP   3 VAL A  345    PRO A  346          0        -8.23
CISPEP   4 TRP B   91    PRO B   92          0        -1.47
CISPEP   5 ALA B  129    PRO B  130          0         4.03
CISPEP   6 VAL B  345    PRO B  346          0        -6.19
SITE     1 AC1 10 GLY A  76  GLY A  77  SER A 163  ALA A 164
SITE     2 AC1 10 LYS A 343  PHE A 348  GLU A 357  ASP A 421
SITE     3 AC1 10 HIS A 451  HOH A 648
SITE     1 AC2  8 TRP A 218  LYS A 262  ALA A 287  GLY A 288
SITE     2 AC2  8 ARG A 293  SER A 333  LEU A 336  HOH A 914
SITE     1 AC3  4 GLN A 100  GLY A 458  HOH A 953  GLU B 244
SITE     1 AC4 10 GLY B  76  GLY B  77  SER B 163  ALA B 164
SITE     2 AC4 10 LYS B 343  PHE B 348  GLU B 357  ASP B 421
SITE     3 AC4 10 HIS B 451  HOH B 659
SITE     1 AC5  8 ALA B  65  SER B 122  GLY B 123  GLN B 124
SITE     2 AC5  8 ASN B 155  HOH B 855  HOH B 979  HOH B 987
SITE     1 AC6  7 TRP B 218  LYS B 262  ALA B 287  GLY B 288
SITE     2 AC6  7 ARG B 293  LEU B 336  HOH B 799
SITE     1 AC7  2 GLU B 435  HOH B 880
SITE     1 AC8  6 GLU B 389  GLN B 392  SER B 398  TYR B 399
SITE     2 AC8  6 SER B 404  HOH B 836
SITE     1 AC9  5 PRO B  14  GLU B  15  GLN B  25  TYR B  27
SITE     2 AC9  5 HOH B 921
SITE     1 BC1  6 ALA B  40  ALA B  41  LYS B 121  HOH B 768
SITE     2 BC1  6 HOH B 934  HOH B 949
CRYST1   46.578   62.852   83.853  70.35  86.05  79.48 P 1           2
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.021469 -0.003988 -0.000177        0.00000
SCALE2      0.000000  0.016183 -0.005659        0.00000
SCALE3      0.000000  0.000000  0.012664        0.00000
TER    3515      GLN A 469
TER    7236      GLN B 469
MASTER      565    0   10   38   26    0   20    6 7883    2   84   76
END