longtext: 4KEA-pdb

content
HEADER    HYDROLASE                               25-APR-13   4KEA
TITLE     CRYSTAL STRUCTURE OF D196N MUTANT OF MONOGLYCERIDE LIPASE FROM
TITLE    2 BACILLUS SP. H257 IN SPACE GROUP P212121
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: THERMOSTABLE MONOACYLGLYCEROL LIPASE;
COMPND   3 CHAIN: A, B, C, D, E, F;
COMPND   4 SYNONYM: MGLP;
COMPND   5 EC: 3.1.1.23;
COMPND   6 ENGINEERED: YES;
COMPND   7 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: BACILLUS SP.;
SOURCE   3 ORGANISM_TAXID: 129908;
SOURCE   4 STRAIN: H-257;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PET28A(+)
KEYWDS    ALPHA/BETA HYDROLASE, MONOGLYCERIDE LIPASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    S.RENGACHARI,P.ASCHAUER,K.GRUBER,I.DREVENY,M.OBERER
REVDAT   1   18-SEP-13 4KEA    0
JRNL        AUTH   S.RENGACHARI,P.ASCHAUER,K.GRUBER,I.DREVENY,M.OBERER
JRNL        TITL   CONFORMATIONAL PLASTICITY AND LIGAND BINDING OF BACTERIAL
JRNL        TITL 2 MONOACYLGLYCEROL LIPASE
JRNL        REF    J.BIOL.CHEM.                               2013
JRNL        REFN                   ESSN 1083-351X
REMARK   2
REMARK   2 RESOLUTION.    1.70 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.8_1069)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER
REMARK   3               : ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.70
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.88
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340
REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.0
REMARK   3   NUMBER OF REFLECTIONS             : 195079
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.192
REMARK   3   R VALUE            (WORKING SET) : 0.191
REMARK   3   FREE R VALUE                     : 0.211
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.040
REMARK   3   FREE R VALUE TEST SET COUNT      : 9832
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 19.8829 -  5.2508    1.00     6666   382  0.1797 0.1912
REMARK   3     2  5.2508 -  4.1804    1.00     6379   348  0.1461 0.1585
REMARK   3     3  4.1804 -  3.6557    1.00     6303   359  0.1515 0.1678
REMARK   3     4  3.6557 -  3.3232    1.00     6259   340  0.1660 0.1876
REMARK   3     5  3.3232 -  3.0859    1.00     6255   341  0.1771 0.1961
REMARK   3     6  3.0859 -  2.9045    1.00     6224   321  0.1817 0.2002
REMARK   3     7  2.9045 -  2.7595    1.00     6184   340  0.1892 0.2125
REMARK   3     8  2.7595 -  2.6396    1.00     6202   312  0.1911 0.2031
REMARK   3     9  2.6396 -  2.5382    1.00     6218   327  0.1954 0.2073
REMARK   3    10  2.5382 -  2.4508    1.00     6072   343  0.1984 0.2281
REMARK   3    11  2.4508 -  2.3743    1.00     6240   332  0.2029 0.2242
REMARK   3    12  2.3743 -  2.3065    1.00     6056   334  0.2018 0.2247
REMARK   3    13  2.3065 -  2.2459    1.00     6275   319  0.2009 0.2404
REMARK   3    14  2.2459 -  2.1912    1.00     6060   331  0.2098 0.2289
REMARK   3    15  2.1912 -  2.1414    1.00     6187   337  0.2063 0.2387
REMARK   3    16  2.1414 -  2.0959    1.00     6165   294  0.2188 0.2608
REMARK   3    17  2.0959 -  2.0540    1.00     6033   349  0.2252 0.2459
REMARK   3    18  2.0540 -  2.0153    1.00     6210   322  0.2178 0.2487
REMARK   3    19  2.0153 -  1.9793    1.00     6108   330  0.2228 0.2219
REMARK   3    20  1.9793 -  1.9458    1.00     6128   280  0.2316 0.2602
REMARK   3    21  1.9458 -  1.9145    1.00     6168   332  0.2312 0.2486
REMARK   3    22  1.9145 -  1.8850    1.00     6110   301  0.2274 0.2632
REMARK   3    23  1.8850 -  1.8573    1.00     6069   320  0.2309 0.2397
REMARK   3    24  1.8573 -  1.8312    1.00     6174   330  0.2346 0.2536
REMARK   3    25  1.8312 -  1.8064    1.00     6142   293  0.2412 0.2781
REMARK   3    26  1.8064 -  1.7830    1.00     5965   318  0.2456 0.2674
REMARK   3    27  1.7830 -  1.7607    1.00     6166   334  0.2526 0.2802
REMARK   3    28  1.7607 -  1.7395    1.00     6169   328  0.2589 0.3249
REMARK   3    29  1.7395 -  1.7193    1.00     6076   301  0.2668 0.2865
REMARK   3    30  1.7193 -  1.7000    1.00     5984   334  0.2679 0.2999
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.160
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 20.470
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 28.18
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.006          12049
REMARK   3   ANGLE     :  0.954          16465
REMARK   3   CHIRALITY :  0.180           1872
REMARK   3   PLANARITY :  0.005           2109
REMARK   3   DIHEDRAL  : 13.111           4438
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 4KEA COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 02-MAY-13.
REMARK 100 THE RCSB ID CODE IS RCSB079229.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 04-AUG-12
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 6.9
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SLS
REMARK 200  BEAMLINE                       : X06DA
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.0
REMARK 200  MONOCHROMATOR                  : SI(111)
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 2M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XSCALE
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 195210
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.700
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9
REMARK 200  DATA REDUNDANCY                : 8.200
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : 0.05100
REMARK 200   FOR THE DATA SET  : 22.6000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.70
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.79
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.60
REMARK 200  R MERGE FOR SHELL          (I) : 0.40600
REMARK 200  R SYM FOR SHELL            (I) : 0.40600
REMARK 200   FOR SHELL         : 1.900
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 50.28
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.47
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M HEPES PH 6.9, 54% V/V MPD, VAPOR
REMARK 280  DIFFUSION, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       19.58250
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      122.35200
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       91.56550
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000      122.35200
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       19.58250
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       91.56550
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4, 5, 6
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 5
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: E
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 6
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: F
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A   -19
REMARK 465     GLY A   -18
REMARK 465     SER A   -17
REMARK 465     SER A   -16
REMARK 465     HIS A   -15
REMARK 465     HIS A   -14
REMARK 465     HIS A   -13
REMARK 465     HIS A   -12
REMARK 465     HIS A   -11
REMARK 465     HIS A   -10
REMARK 465     SER A    -9
REMARK 465     SER A    -8
REMARK 465     GLY A    -7
REMARK 465     LEU A    -6
REMARK 465     VAL A    -5
REMARK 465     PRO A    -4
REMARK 465     ARG A    -3
REMARK 465     GLY A    -2
REMARK 465     SER A    -1
REMARK 465     HIS A     0
REMARK 465     MET A     1
REMARK 465     GLY A   250
REMARK 465     MET B   -19
REMARK 465     GLY B   -18
REMARK 465     SER B   -17
REMARK 465     SER B   -16
REMARK 465     HIS B   -15
REMARK 465     HIS B   -14
REMARK 465     HIS B   -13
REMARK 465     HIS B   -12
REMARK 465     HIS B   -11
REMARK 465     HIS B   -10
REMARK 465     SER B    -9
REMARK 465     SER B    -8
REMARK 465     GLY B    -7
REMARK 465     LEU B    -6
REMARK 465     VAL B    -5
REMARK 465     PRO B    -4
REMARK 465     ARG B    -3
REMARK 465     GLY B    -2
REMARK 465     SER B    -1
REMARK 465     HIS B     0
REMARK 465     MET B     1
REMARK 465     GLY B   131
REMARK 465     MET B   132
REMARK 465     THR B   133
REMARK 465     GLY B   134
REMARK 465     GLY B   135
REMARK 465     GLY B   136
REMARK 465     MET C   -19
REMARK 465     GLY C   -18
REMARK 465     SER C   -17
REMARK 465     SER C   -16
REMARK 465     HIS C   -15
REMARK 465     HIS C   -14
REMARK 465     HIS C   -13
REMARK 465     HIS C   -12
REMARK 465     HIS C   -11
REMARK 465     HIS C   -10
REMARK 465     SER C    -9
REMARK 465     SER C    -8
REMARK 465     GLY C    -7
REMARK 465     LEU C    -6
REMARK 465     VAL C    -5
REMARK 465     PRO C    -4
REMARK 465     ARG C    -3
REMARK 465     GLY C    -2
REMARK 465     SER C    -1
REMARK 465     HIS C     0
REMARK 465     MET C     1
REMARK 465     THR C   133
REMARK 465     GLY C   134
REMARK 465     GLY C   135
REMARK 465     GLY C   250
REMARK 465     MET D   -19
REMARK 465     GLY D   -18
REMARK 465     SER D   -17
REMARK 465     SER D   -16
REMARK 465     HIS D   -15
REMARK 465     HIS D   -14
REMARK 465     HIS D   -13
REMARK 465     HIS D   -12
REMARK 465     HIS D   -11
REMARK 465     HIS D   -10
REMARK 465     SER D    -9
REMARK 465     SER D    -8
REMARK 465     GLY D    -7
REMARK 465     LEU D    -6
REMARK 465     VAL D    -5
REMARK 465     PRO D    -4
REMARK 465     ARG D    -3
REMARK 465     GLY D    -2
REMARK 465     SER D    -1
REMARK 465     HIS D     0
REMARK 465     MET D     1
REMARK 465     GLY D   131
REMARK 465     MET D   132
REMARK 465     THR D   133
REMARK 465     GLY D   134
REMARK 465     GLY D   250
REMARK 465     MET E   -19
REMARK 465     GLY E   -18
REMARK 465     SER E   -17
REMARK 465     SER E   -16
REMARK 465     HIS E   -15
REMARK 465     HIS E   -14
REMARK 465     HIS E   -13
REMARK 465     HIS E   -12
REMARK 465     HIS E   -11
REMARK 465     HIS E   -10
REMARK 465     SER E    -9
REMARK 465     SER E    -8
REMARK 465     GLY E    -7
REMARK 465     LEU E    -6
REMARK 465     VAL E    -5
REMARK 465     PRO E    -4
REMARK 465     ARG E    -3
REMARK 465     GLY E    -2
REMARK 465     SER E    -1
REMARK 465     HIS E     0
REMARK 465     MET E     1
REMARK 465     GLY E   131
REMARK 465     MET E   132
REMARK 465     THR E   133
REMARK 465     GLY E   134
REMARK 465     GLY E   135
REMARK 465     GLY E   136
REMARK 465     GLU E   137
REMARK 465     MET F   -19
REMARK 465     GLY F   -18
REMARK 465     SER F   -17
REMARK 465     SER F   -16
REMARK 465     HIS F   -15
REMARK 465     HIS F   -14
REMARK 465     HIS F   -13
REMARK 465     HIS F   -12
REMARK 465     HIS F   -11
REMARK 465     HIS F   -10
REMARK 465     SER F    -9
REMARK 465     SER F    -8
REMARK 465     GLY F    -7
REMARK 465     LEU F    -6
REMARK 465     VAL F    -5
REMARK 465     PRO F    -4
REMARK 465     ARG F    -3
REMARK 465     GLY F    -2
REMARK 465     SER F    -1
REMARK 465     HIS F     0
REMARK 465     MET F     1
REMARK 465     SER F     2
REMARK 465     ALA F   130
REMARK 465     GLY F   131
REMARK 465     MET F   132
REMARK 465     THR F   133
REMARK 465     GLY F   134
REMARK 465     GLY F   135
REMARK 465     GLY F   136
REMARK 465     GLU F   137
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OE2  GLU E   156     O4   MPD E   302              1.96
REMARK 500   OG   SER E   147     OE1  GLU E   156              2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    CYS B 114   CA  -  CB  -  SG  ANGL. DEV. =   7.6 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASN A  18     -111.78   -129.73
REMARK 500    THR A  30       -3.05     70.87
REMARK 500    THR A  60      -78.54   -122.71
REMARK 500    SER A  97     -116.44     61.22
REMARK 500    VAL A 198      -60.24   -124.08
REMARK 500    TYR A 225     -149.48    -95.81
REMARK 500    ASN B  18     -108.32   -133.67
REMARK 500    THR B  30       -3.68     69.80
REMARK 500    THR B  60      -78.54   -127.39
REMARK 500    SER B  97     -116.51     62.40
REMARK 500    ASP B 194      -14.27    -41.63
REMARK 500    VAL B 198      -58.54   -122.36
REMARK 500    TYR B 225     -151.13    -97.34
REMARK 500    ASN C  18     -110.54   -131.24
REMARK 500    THR C  30       -1.12     68.20
REMARK 500    THR C  60      -80.99   -127.81
REMARK 500    SER C  97     -115.70     60.70
REMARK 500    ALA C 130       46.11    -91.19
REMARK 500    VAL C 198      -58.56   -123.26
REMARK 500    TYR C 225     -146.61    -96.74
REMARK 500    ASN D  18     -115.47   -130.22
REMARK 500    THR D  30       -2.59     68.35
REMARK 500    THR D  60      -79.61   -125.82
REMARK 500    SER D  97     -115.39     60.37
REMARK 500    ASP D 153       35.87    -99.32
REMARK 500    VAL D 198      -58.10   -123.72
REMARK 500    TYR D 225     -147.39    -99.05
REMARK 500    ASN E  18     -117.80   -129.41
REMARK 500    THR E  30       -0.95     69.17
REMARK 500    THR E  60      -82.14   -123.55
REMARK 500    SER E  97     -113.71     60.80
REMARK 500    ASP E 153       37.79    -82.28
REMARK 500    VAL E 198      -59.05   -121.67
REMARK 500    TYR E 225     -146.33    -98.55
REMARK 500    ASN F  18     -111.13   -131.50
REMARK 500    THR F  60      -78.57   -126.55
REMARK 500    SER F  97     -113.49     58.55
REMARK 500    ALA F 127       41.51    -68.19
REMARK 500    ILE F 128      -36.89   -137.78
REMARK 500    LEU F 173      -54.10    -23.38
REMARK 500    VAL F 198      -56.88   -125.05
REMARK 500    PRO F 201       -7.20    -57.03
REMARK 500    TYR F 225     -148.77    -92.69
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD A 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD A 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD A 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD A 304
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD A 305
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD A 306
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD B 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD B 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD B 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD B 304
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD C 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD C 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD C 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD C 304
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD D 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD D 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD D 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD E 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: CC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD E 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: CC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD E 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: CC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD E 304
REMARK 800
REMARK 800 SITE_IDENTIFIER: CC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD F 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: CC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD F 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: CC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD F 303
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4KE6   RELATED DB: PDB
REMARK 900 RELATED ID: 4KE7   RELATED DB: PDB
REMARK 900 RELATED ID: 4KE8   RELATED DB: PDB
REMARK 900 RELATED ID: 4KE9   RELATED DB: PDB
DBREF  4KEA A    1   250  UNP    P82597   MGLP_BAC25       1    250
DBREF  4KEA B    1   250  UNP    P82597   MGLP_BAC25       1    250
DBREF  4KEA C    1   250  UNP    P82597   MGLP_BAC25       1    250
DBREF  4KEA D    1   250  UNP    P82597   MGLP_BAC25       1    250
DBREF  4KEA E    1   250  UNP    P82597   MGLP_BAC25       1    250
DBREF  4KEA F    1   250  UNP    P82597   MGLP_BAC25       1    250
SEQADV 4KEA MET A  -19  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY A  -18  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER A  -17  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER A  -16  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS A  -15  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS A  -14  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS A  -13  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS A  -12  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS A  -11  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS A  -10  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER A   -9  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER A   -8  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY A   -7  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA LEU A   -6  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA VAL A   -5  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA PRO A   -4  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ARG A   -3  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY A   -2  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER A   -1  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS A    0  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ASN A  196  UNP  P82597    ASP   196 ENGINEERED MUTATION
SEQADV 4KEA MET B  -19  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY B  -18  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER B  -17  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER B  -16  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS B  -15  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS B  -14  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS B  -13  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS B  -12  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS B  -11  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS B  -10  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER B   -9  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER B   -8  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY B   -7  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA LEU B   -6  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA VAL B   -5  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA PRO B   -4  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ARG B   -3  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY B   -2  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER B   -1  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS B    0  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ASN B  196  UNP  P82597    ASP   196 ENGINEERED MUTATION
SEQADV 4KEA MET C  -19  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY C  -18  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER C  -17  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER C  -16  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS C  -15  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS C  -14  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS C  -13  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS C  -12  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS C  -11  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS C  -10  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER C   -9  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER C   -8  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY C   -7  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA LEU C   -6  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA VAL C   -5  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA PRO C   -4  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ARG C   -3  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY C   -2  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER C   -1  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS C    0  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ASN C  196  UNP  P82597    ASP   196 ENGINEERED MUTATION
SEQADV 4KEA MET D  -19  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY D  -18  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER D  -17  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER D  -16  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS D  -15  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS D  -14  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS D  -13  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS D  -12  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS D  -11  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS D  -10  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER D   -9  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER D   -8  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY D   -7  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA LEU D   -6  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA VAL D   -5  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA PRO D   -4  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ARG D   -3  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY D   -2  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER D   -1  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS D    0  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ASN D  196  UNP  P82597    ASP   196 ENGINEERED MUTATION
SEQADV 4KEA MET E  -19  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY E  -18  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER E  -17  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER E  -16  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS E  -15  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS E  -14  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS E  -13  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS E  -12  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS E  -11  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS E  -10  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER E   -9  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER E   -8  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY E   -7  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA LEU E   -6  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA VAL E   -5  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA PRO E   -4  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ARG E   -3  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY E   -2  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER E   -1  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS E    0  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ASN E  196  UNP  P82597    ASP   196 ENGINEERED MUTATION
SEQADV 4KEA MET F  -19  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY F  -18  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER F  -17  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER F  -16  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS F  -15  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS F  -14  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS F  -13  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS F  -12  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS F  -11  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS F  -10  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER F   -9  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER F   -8  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY F   -7  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA LEU F   -6  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA VAL F   -5  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA PRO F   -4  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ARG F   -3  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA GLY F   -2  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA SER F   -1  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA HIS F    0  UNP  P82597              EXPRESSION TAG
SEQADV 4KEA ASN F  196  UNP  P82597    ASP   196 ENGINEERED MUTATION
SEQRES   1 A  270  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 A  270  LEU VAL PRO ARG GLY SER HIS MET SER GLU GLN TYR PRO
SEQRES   3 A  270  VAL LEU SER GLY ALA GLU PRO PHE TYR ALA GLU ASN GLY
SEQRES   4 A  270  PRO VAL GLY VAL LEU LEU VAL HIS GLY PHE THR GLY THR
SEQRES   5 A  270  PRO HIS SER MET ARG PRO LEU ALA GLU ALA TYR ALA LYS
SEQRES   6 A  270  ALA GLY TYR THR VAL CYS LEU PRO ARG LEU LYS GLY HIS
SEQRES   7 A  270  GLY THR HIS TYR GLU ASP MET GLU ARG THR THR PHE HIS
SEQRES   8 A  270  ASP TRP VAL ALA SER VAL GLU GLU GLY TYR GLY TRP LEU
SEQRES   9 A  270  LYS GLN ARG CYS GLN THR ILE PHE VAL THR GLY LEU SER
SEQRES  10 A  270  MET GLY GLY THR LEU THR LEU TYR LEU ALA GLU HIS HIS
SEQRES  11 A  270  PRO ASP ILE CYS GLY ILE VAL PRO ILE ASN ALA ALA VAL
SEQRES  12 A  270  ASP ILE PRO ALA ILE ALA ALA GLY MET THR GLY GLY GLY
SEQRES  13 A  270  GLU LEU PRO ARG TYR LEU ASP SER ILE GLY SER ASP LEU
SEQRES  14 A  270  LYS ASN PRO ASP VAL LYS GLU LEU ALA TYR GLU LYS THR
SEQRES  15 A  270  PRO THR ALA SER LEU LEU GLN LEU ALA ARG LEU MET ALA
SEQRES  16 A  270  GLN THR LYS ALA LYS LEU ASP ARG ILE VAL CYS PRO ALA
SEQRES  17 A  270  LEU ILE PHE VAL SER ASP GLU ASN HIS VAL VAL PRO PRO
SEQRES  18 A  270  GLY ASN ALA ASP ILE ILE PHE GLN GLY ILE SER SER THR
SEQRES  19 A  270  GLU LYS GLU ILE VAL ARG LEU ARG ASN SER TYR HIS VAL
SEQRES  20 A  270  ALA THR LEU ASP TYR ASP GLN PRO MET ILE ILE GLU ARG
SEQRES  21 A  270  SER LEU GLU PHE PHE ALA LYS HIS ALA GLY
SEQRES   1 B  270  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 B  270  LEU VAL PRO ARG GLY SER HIS MET SER GLU GLN TYR PRO
SEQRES   3 B  270  VAL LEU SER GLY ALA GLU PRO PHE TYR ALA GLU ASN GLY
SEQRES   4 B  270  PRO VAL GLY VAL LEU LEU VAL HIS GLY PHE THR GLY THR
SEQRES   5 B  270  PRO HIS SER MET ARG PRO LEU ALA GLU ALA TYR ALA LYS
SEQRES   6 B  270  ALA GLY TYR THR VAL CYS LEU PRO ARG LEU LYS GLY HIS
SEQRES   7 B  270  GLY THR HIS TYR GLU ASP MET GLU ARG THR THR PHE HIS
SEQRES   8 B  270  ASP TRP VAL ALA SER VAL GLU GLU GLY TYR GLY TRP LEU
SEQRES   9 B  270  LYS GLN ARG CYS GLN THR ILE PHE VAL THR GLY LEU SER
SEQRES  10 B  270  MET GLY GLY THR LEU THR LEU TYR LEU ALA GLU HIS HIS
SEQRES  11 B  270  PRO ASP ILE CYS GLY ILE VAL PRO ILE ASN ALA ALA VAL
SEQRES  12 B  270  ASP ILE PRO ALA ILE ALA ALA GLY MET THR GLY GLY GLY
SEQRES  13 B  270  GLU LEU PRO ARG TYR LEU ASP SER ILE GLY SER ASP LEU
SEQRES  14 B  270  LYS ASN PRO ASP VAL LYS GLU LEU ALA TYR GLU LYS THR
SEQRES  15 B  270  PRO THR ALA SER LEU LEU GLN LEU ALA ARG LEU MET ALA
SEQRES  16 B  270  GLN THR LYS ALA LYS LEU ASP ARG ILE VAL CYS PRO ALA
SEQRES  17 B  270  LEU ILE PHE VAL SER ASP GLU ASN HIS VAL VAL PRO PRO
SEQRES  18 B  270  GLY ASN ALA ASP ILE ILE PHE GLN GLY ILE SER SER THR
SEQRES  19 B  270  GLU LYS GLU ILE VAL ARG LEU ARG ASN SER TYR HIS VAL
SEQRES  20 B  270  ALA THR LEU ASP TYR ASP GLN PRO MET ILE ILE GLU ARG
SEQRES  21 B  270  SER LEU GLU PHE PHE ALA LYS HIS ALA GLY
SEQRES   1 C  270  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 C  270  LEU VAL PRO ARG GLY SER HIS MET SER GLU GLN TYR PRO
SEQRES   3 C  270  VAL LEU SER GLY ALA GLU PRO PHE TYR ALA GLU ASN GLY
SEQRES   4 C  270  PRO VAL GLY VAL LEU LEU VAL HIS GLY PHE THR GLY THR
SEQRES   5 C  270  PRO HIS SER MET ARG PRO LEU ALA GLU ALA TYR ALA LYS
SEQRES   6 C  270  ALA GLY TYR THR VAL CYS LEU PRO ARG LEU LYS GLY HIS
SEQRES   7 C  270  GLY THR HIS TYR GLU ASP MET GLU ARG THR THR PHE HIS
SEQRES   8 C  270  ASP TRP VAL ALA SER VAL GLU GLU GLY TYR GLY TRP LEU
SEQRES   9 C  270  LYS GLN ARG CYS GLN THR ILE PHE VAL THR GLY LEU SER
SEQRES  10 C  270  MET GLY GLY THR LEU THR LEU TYR LEU ALA GLU HIS HIS
SEQRES  11 C  270  PRO ASP ILE CYS GLY ILE VAL PRO ILE ASN ALA ALA VAL
SEQRES  12 C  270  ASP ILE PRO ALA ILE ALA ALA GLY MET THR GLY GLY GLY
SEQRES  13 C  270  GLU LEU PRO ARG TYR LEU ASP SER ILE GLY SER ASP LEU
SEQRES  14 C  270  LYS ASN PRO ASP VAL LYS GLU LEU ALA TYR GLU LYS THR
SEQRES  15 C  270  PRO THR ALA SER LEU LEU GLN LEU ALA ARG LEU MET ALA
SEQRES  16 C  270  GLN THR LYS ALA LYS LEU ASP ARG ILE VAL CYS PRO ALA
SEQRES  17 C  270  LEU ILE PHE VAL SER ASP GLU ASN HIS VAL VAL PRO PRO
SEQRES  18 C  270  GLY ASN ALA ASP ILE ILE PHE GLN GLY ILE SER SER THR
SEQRES  19 C  270  GLU LYS GLU ILE VAL ARG LEU ARG ASN SER TYR HIS VAL
SEQRES  20 C  270  ALA THR LEU ASP TYR ASP GLN PRO MET ILE ILE GLU ARG
SEQRES  21 C  270  SER LEU GLU PHE PHE ALA LYS HIS ALA GLY
SEQRES   1 D  270  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 D  270  LEU VAL PRO ARG GLY SER HIS MET SER GLU GLN TYR PRO
SEQRES   3 D  270  VAL LEU SER GLY ALA GLU PRO PHE TYR ALA GLU ASN GLY
SEQRES   4 D  270  PRO VAL GLY VAL LEU LEU VAL HIS GLY PHE THR GLY THR
SEQRES   5 D  270  PRO HIS SER MET ARG PRO LEU ALA GLU ALA TYR ALA LYS
SEQRES   6 D  270  ALA GLY TYR THR VAL CYS LEU PRO ARG LEU LYS GLY HIS
SEQRES   7 D  270  GLY THR HIS TYR GLU ASP MET GLU ARG THR THR PHE HIS
SEQRES   8 D  270  ASP TRP VAL ALA SER VAL GLU GLU GLY TYR GLY TRP LEU
SEQRES   9 D  270  LYS GLN ARG CYS GLN THR ILE PHE VAL THR GLY LEU SER
SEQRES  10 D  270  MET GLY GLY THR LEU THR LEU TYR LEU ALA GLU HIS HIS
SEQRES  11 D  270  PRO ASP ILE CYS GLY ILE VAL PRO ILE ASN ALA ALA VAL
SEQRES  12 D  270  ASP ILE PRO ALA ILE ALA ALA GLY MET THR GLY GLY GLY
SEQRES  13 D  270  GLU LEU PRO ARG TYR LEU ASP SER ILE GLY SER ASP LEU
SEQRES  14 D  270  LYS ASN PRO ASP VAL LYS GLU LEU ALA TYR GLU LYS THR
SEQRES  15 D  270  PRO THR ALA SER LEU LEU GLN LEU ALA ARG LEU MET ALA
SEQRES  16 D  270  GLN THR LYS ALA LYS LEU ASP ARG ILE VAL CYS PRO ALA
SEQRES  17 D  270  LEU ILE PHE VAL SER ASP GLU ASN HIS VAL VAL PRO PRO
SEQRES  18 D  270  GLY ASN ALA ASP ILE ILE PHE GLN GLY ILE SER SER THR
SEQRES  19 D  270  GLU LYS GLU ILE VAL ARG LEU ARG ASN SER TYR HIS VAL
SEQRES  20 D  270  ALA THR LEU ASP TYR ASP GLN PRO MET ILE ILE GLU ARG
SEQRES  21 D  270  SER LEU GLU PHE PHE ALA LYS HIS ALA GLY
SEQRES   1 E  270  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 E  270  LEU VAL PRO ARG GLY SER HIS MET SER GLU GLN TYR PRO
SEQRES   3 E  270  VAL LEU SER GLY ALA GLU PRO PHE TYR ALA GLU ASN GLY
SEQRES   4 E  270  PRO VAL GLY VAL LEU LEU VAL HIS GLY PHE THR GLY THR
SEQRES   5 E  270  PRO HIS SER MET ARG PRO LEU ALA GLU ALA TYR ALA LYS
SEQRES   6 E  270  ALA GLY TYR THR VAL CYS LEU PRO ARG LEU LYS GLY HIS
SEQRES   7 E  270  GLY THR HIS TYR GLU ASP MET GLU ARG THR THR PHE HIS
SEQRES   8 E  270  ASP TRP VAL ALA SER VAL GLU GLU GLY TYR GLY TRP LEU
SEQRES   9 E  270  LYS GLN ARG CYS GLN THR ILE PHE VAL THR GLY LEU SER
SEQRES  10 E  270  MET GLY GLY THR LEU THR LEU TYR LEU ALA GLU HIS HIS
SEQRES  11 E  270  PRO ASP ILE CYS GLY ILE VAL PRO ILE ASN ALA ALA VAL
SEQRES  12 E  270  ASP ILE PRO ALA ILE ALA ALA GLY MET THR GLY GLY GLY
SEQRES  13 E  270  GLU LEU PRO ARG TYR LEU ASP SER ILE GLY SER ASP LEU
SEQRES  14 E  270  LYS ASN PRO ASP VAL LYS GLU LEU ALA TYR GLU LYS THR
SEQRES  15 E  270  PRO THR ALA SER LEU LEU GLN LEU ALA ARG LEU MET ALA
SEQRES  16 E  270  GLN THR LYS ALA LYS LEU ASP ARG ILE VAL CYS PRO ALA
SEQRES  17 E  270  LEU ILE PHE VAL SER ASP GLU ASN HIS VAL VAL PRO PRO
SEQRES  18 E  270  GLY ASN ALA ASP ILE ILE PHE GLN GLY ILE SER SER THR
SEQRES  19 E  270  GLU LYS GLU ILE VAL ARG LEU ARG ASN SER TYR HIS VAL
SEQRES  20 E  270  ALA THR LEU ASP TYR ASP GLN PRO MET ILE ILE GLU ARG
SEQRES  21 E  270  SER LEU GLU PHE PHE ALA LYS HIS ALA GLY
SEQRES   1 F  270  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 F  270  LEU VAL PRO ARG GLY SER HIS MET SER GLU GLN TYR PRO
SEQRES   3 F  270  VAL LEU SER GLY ALA GLU PRO PHE TYR ALA GLU ASN GLY
SEQRES   4 F  270  PRO VAL GLY VAL LEU LEU VAL HIS GLY PHE THR GLY THR
SEQRES   5 F  270  PRO HIS SER MET ARG PRO LEU ALA GLU ALA TYR ALA LYS
SEQRES   6 F  270  ALA GLY TYR THR VAL CYS LEU PRO ARG LEU LYS GLY HIS
SEQRES   7 F  270  GLY THR HIS TYR GLU ASP MET GLU ARG THR THR PHE HIS
SEQRES   8 F  270  ASP TRP VAL ALA SER VAL GLU GLU GLY TYR GLY TRP LEU
SEQRES   9 F  270  LYS GLN ARG CYS GLN THR ILE PHE VAL THR GLY LEU SER
SEQRES  10 F  270  MET GLY GLY THR LEU THR LEU TYR LEU ALA GLU HIS HIS
SEQRES  11 F  270  PRO ASP ILE CYS GLY ILE VAL PRO ILE ASN ALA ALA VAL
SEQRES  12 F  270  ASP ILE PRO ALA ILE ALA ALA GLY MET THR GLY GLY GLY
SEQRES  13 F  270  GLU LEU PRO ARG TYR LEU ASP SER ILE GLY SER ASP LEU
SEQRES  14 F  270  LYS ASN PRO ASP VAL LYS GLU LEU ALA TYR GLU LYS THR
SEQRES  15 F  270  PRO THR ALA SER LEU LEU GLN LEU ALA ARG LEU MET ALA
SEQRES  16 F  270  GLN THR LYS ALA LYS LEU ASP ARG ILE VAL CYS PRO ALA
SEQRES  17 F  270  LEU ILE PHE VAL SER ASP GLU ASN HIS VAL VAL PRO PRO
SEQRES  18 F  270  GLY ASN ALA ASP ILE ILE PHE GLN GLY ILE SER SER THR
SEQRES  19 F  270  GLU LYS GLU ILE VAL ARG LEU ARG ASN SER TYR HIS VAL
SEQRES  20 F  270  ALA THR LEU ASP TYR ASP GLN PRO MET ILE ILE GLU ARG
SEQRES  21 F  270  SER LEU GLU PHE PHE ALA LYS HIS ALA GLY
HET    MPD  A 301       8
HET    MPD  A 302       8
HET    MPD  A 303       8
HET    MPD  A 304       8
HET    MPD  A 305       8
HET    MPD  A 306       8
HET    MPD  B 301       8
HET    MPD  B 302       8
HET    MPD  B 303       8
HET    MPD  B 304       8
HET    MPD  C 301       8
HET    MPD  C 302       8
HET    MPD  C 303       8
HET    MPD  C 304       8
HET    MPD  D 301       8
HET    MPD  D 302       8
HET    MPD  D 303       8
HET    MPD  E 301       8
HET    MPD  E 302       8
HET    MPD  E 303       8
HET    MPD  E 304       8
HET    MPD  F 301       8
HET    MPD  F 302       8
HET    MPD  F 303       8
HETNAM     MPD (4S)-2-METHYL-2,4-PENTANEDIOL
FORMUL   7  MPD    24(C6 H14 O2)
FORMUL  31  HOH   *904(H2 O)
HELIX    1   1 THR A   32  SER A   35  5                                   4
HELIX    2   2 MET A   36  ALA A   46  1                                  11
HELIX    3   3 HIS A   61  THR A   68  1                                   8
HELIX    4   4 THR A   69  GLN A   86  1                                  18
HELIX    5   5 SER A   97  HIS A  110  1                                  14
HELIX    6   6 ILE A  125  GLY A  134  1                                  10
HELIX    7   7 THR A  164  LYS A  180  1                                  17
HELIX    8   8 LEU A  181  ILE A  184  5                                   4
HELIX    9   9 GLY A  202  ILE A  211  1                                  10
HELIX   10  10 VAL A  227  ASP A  231  5                                   5
HELIX   11  11 ASP A  233  ALA A  249  1                                  17
HELIX   12  12 THR B   32  SER B   35  5                                   4
HELIX   13  13 MET B   36  ALA B   46  1                                  11
HELIX   14  14 HIS B   61  ARG B   67  1                                   7
HELIX   15  15 THR B   69  GLN B   86  1                                  18
HELIX   16  16 SER B   97  HIS B  110  1                                  14
HELIX   17  17 ILE B  125  ALA B  130  1                                   6
HELIX   18  18 THR B  164  LYS B  180  1                                  17
HELIX   19  19 LEU B  181  ILE B  184  5                                   4
HELIX   20  20 GLY B  202  ILE B  211  1                                  10
HELIX   21  21 VAL B  227  ASP B  231  5                                   5
HELIX   22  22 ASP B  233  GLY B  250  1                                  18
HELIX   23  23 THR C   32  SER C   35  5                                   4
HELIX   24  24 MET C   36  ALA C   46  1                                  11
HELIX   25  25 HIS C   61  ARG C   67  1                                   7
HELIX   26  26 THR C   69  GLN C   86  1                                  18
HELIX   27  27 SER C   97  GLU C  108  1                                  12
HELIX   28  28 ILE C  125  ALA C  130  1                                   6
HELIX   29  29 THR C  164  LYS C  180  1                                  17
HELIX   30  30 LEU C  181  ILE C  184  5                                   4
HELIX   31  31 GLY C  202  ILE C  211  1                                  10
HELIX   32  32 VAL C  227  ASP C  231  5                                   5
HELIX   33  33 ASP C  233  ALA C  249  1                                  17
HELIX   34  34 THR D   32  SER D   35  5                                   4
HELIX   35  35 MET D   36  ALA D   46  1                                  11
HELIX   36  36 HIS D   61  ARG D   67  1                                   7
HELIX   37  37 THR D   69  GLN D   86  1                                  18
HELIX   38  38 SER D   97  HIS D  110  1                                  14
HELIX   39  39 ILE D  125  ALA D  130  1                                   6
HELIX   40  40 THR D  164  LYS D  180  1                                  17
HELIX   41  41 LEU D  181  ILE D  184  5                                   4
HELIX   42  42 GLY D  202  ILE D  211  1                                  10
HELIX   43  43 VAL D  227  ASP D  231  5                                   5
HELIX   44  44 ASP D  233  ALA D  249  1                                  17
HELIX   45  45 THR E   32  SER E   35  5                                   4
HELIX   46  46 MET E   36  ALA E   46  1                                  11
HELIX   47  47 HIS E   61  ARG E   67  1                                   7
HELIX   48  48 THR E   69  GLN E   86  1                                  18
HELIX   49  49 SER E   97  HIS E  110  1                                  14
HELIX   50  50 ILE E  125  ALA E  130  5                                   6
HELIX   51  51 THR E  164  LYS E  180  1                                  17
HELIX   52  52 LEU E  181  ILE E  184  5                                   4
HELIX   53  53 GLY E  202  ILE E  211  1                                  10
HELIX   54  54 VAL E  227  ASP E  231  5                                   5
HELIX   55  55 ASP E  233  ALA E  249  1                                  17
HELIX   56  56 THR F   32  SER F   35  5                                   4
HELIX   57  57 MET F   36  ALA F   46  1                                  11
HELIX   58  58 HIS F   61  ARG F   67  1                                   7
HELIX   59  59 THR F   69  GLN F   86  1                                  18
HELIX   60  60 SER F   97  GLU F  108  1                                  12
HELIX   61  61 THR F  164  LYS F  180  1                                  17
HELIX   62  62 LEU F  181  ILE F  184  5                                   4
HELIX   63  63 PRO F  201  ILE F  211  1                                  11
HELIX   64  64 VAL F  227  ASP F  231  5                                   5
HELIX   65  65 ASP F  233  ALA F  249  1                                  17
SHEET    1   A 5 PHE A  14  ALA A  16  0
SHEET    2   A 5 THR A  49  LEU A  52 -1  O  LEU A  52   N  PHE A  14
SHEET    3   A 5 VAL A  21  VAL A  26  1  N  LEU A  25   O  CYS A  51
SHEET    4   A 5 THR A  90  LEU A  96  1  O  THR A  90   N  GLY A  22
SHEET    5   A 5 GLY A 115  ILE A 119  1  O  ILE A 119   N  GLY A  95
SHEET    1   B 2 TYR A 141  ASP A 143  0
SHEET    2   B 2 LYS A 161  PRO A 163 -1  O  THR A 162   N  LEU A 142
SHEET    1   C 4 ALA A 188  SER A 193  0
SHEET    2   C 4 GLU A 215  LEU A 221  1  O  LEU A 221   N  VAL A 192
SHEET    3   C 4 GLU C 215  LEU C 221 -1  O  ILE C 218   N  LYS A 216
SHEET    4   C 4 ALA C 188  SER C 193  1  N  VAL C 192   O  LEU C 221
SHEET    1   D 5 PHE B  14  ALA B  16  0
SHEET    2   D 5 THR B  49  LEU B  52 -1  O  LEU B  52   N  PHE B  14
SHEET    3   D 5 VAL B  21  VAL B  26  1  N  LEU B  25   O  CYS B  51
SHEET    4   D 5 THR B  90  LEU B  96  1  O  THR B  90   N  GLY B  22
SHEET    5   D 5 GLY B 115  ILE B 119  1  O  ILE B 119   N  GLY B  95
SHEET    1   E 2 TYR B 141  ASP B 143  0
SHEET    2   E 2 LYS B 161  PRO B 163 -1  O  THR B 162   N  LEU B 142
SHEET    1   F 2 ALA B 188  SER B 193  0
SHEET    2   F 2 LYS B 216  LEU B 221  1  O  GLU B 217   N  ILE B 190
SHEET    1   G 5 PHE C  14  ALA C  16  0
SHEET    2   G 5 THR C  49  LEU C  52 -1  O  LEU C  52   N  PHE C  14
SHEET    3   G 5 VAL C  21  VAL C  26  1  N  LEU C  25   O  CYS C  51
SHEET    4   G 5 THR C  90  LEU C  96  1  O  THR C  90   N  GLY C  22
SHEET    5   G 5 GLY C 115  ILE C 119  1  O  ILE C 119   N  GLY C  95
SHEET    1   H 2 TYR C 141  ASP C 143  0
SHEET    2   H 2 LYS C 161  PRO C 163 -1  O  THR C 162   N  LEU C 142
SHEET    1   I 5 PHE D  14  ALA D  16  0
SHEET    2   I 5 THR D  49  LEU D  52 -1  O  LEU D  52   N  PHE D  14
SHEET    3   I 5 VAL D  21  VAL D  26  1  N  VAL D  23   O  THR D  49
SHEET    4   I 5 THR D  90  LEU D  96  1  O  THR D  94   N  LEU D  24
SHEET    5   I 5 GLY D 115  ILE D 119  1  O  ILE D 119   N  GLY D  95
SHEET    1   J 2 TYR D 141  ASP D 143  0
SHEET    2   J 2 LYS D 161  PRO D 163 -1  O  THR D 162   N  LEU D 142
SHEET    1   K 2 ALA D 188  SER D 193  0
SHEET    2   K 2 LYS D 216  LEU D 221  1  O  GLU D 217   N  ILE D 190
SHEET    1   L 5 PHE E  14  ALA E  16  0
SHEET    2   L 5 THR E  49  LEU E  52 -1  O  LEU E  52   N  PHE E  14
SHEET    3   L 5 VAL E  21  VAL E  26  1  N  VAL E  23   O  THR E  49
SHEET    4   L 5 THR E  90  LEU E  96  1  O  THR E  90   N  GLY E  22
SHEET    5   L 5 GLY E 115  ILE E 119  1  O  ILE E 119   N  GLY E  95
SHEET    1   M 2 TYR E 141  ASP E 143  0
SHEET    2   M 2 LYS E 161  PRO E 163 -1  O  THR E 162   N  LEU E 142
SHEET    1   N 2 ALA E 188  SER E 193  0
SHEET    2   N 2 LYS E 216  LEU E 221  1  O  LEU E 221   N  VAL E 192
SHEET    1   O 5 PHE F  14  ALA F  16  0
SHEET    2   O 5 THR F  49  LEU F  52 -1  O  LEU F  52   N  PHE F  14
SHEET    3   O 5 VAL F  21  VAL F  26  1  N  VAL F  21   O  THR F  49
SHEET    4   O 5 THR F  90  LEU F  96  1  O  PHE F  92   N  LEU F  24
SHEET    5   O 5 GLY F 115  ILE F 119  1  O  ILE F 119   N  GLY F  95
SHEET    1   P 2 TYR F 141  ASP F 143  0
SHEET    2   P 2 LYS F 161  PRO F 163 -1  O  THR F 162   N  LEU F 142
SHEET    1   Q 2 ALA F 188  SER F 193  0
SHEET    2   Q 2 LYS F 216  LEU F 221  1  O  LEU F 221   N  VAL F 192
SITE     1 AC1  8 PHE A  29  THR A  30  LEU A  96  GLU A 156
SITE     2 AC1  8 ALA A 158  VAL A 227  HOH A 419  HOH A 431
SITE     1 AC2  4 SER A  97  VAL A 198  HOH A 570  MPD B 303
SITE     1 AC3  4 GLU A 239  ARG A 240  GLU A 243  LYS C 247
SITE     1 AC4  4 ASP A 231  TYR A 232  PRO A 235  HOH A 462
SITE     1 AC5  4 VAL A  21  GLY A  47  TYR A  48  ALA A 249
SITE     1 AC6  5 LEU A   8  LEU A 157  ALA A 158  TYR A 159
SITE     2 AC6  5 GLU A 160
SITE     1 AC7  9 GLY B  28  PHE B  29  THR B  30  LEU B  96
SITE     2 AC7  9 GLU B 156  VAL B 227  MPD B 302  HOH B 401
SITE     3 AC7  9 HOH B 411
SITE     1 AC8  4 PHE B  29  SER B  97  MPD B 301  HOH B 402
SITE     1 AC9  5 ALA A 127  MPD A 302  ASP B 143  MPD B 304
SITE     2 AC9  5 HOH B 437
SITE     1 BC1  4 ASP B 143  ILE B 145  MPD B 303  HOH B 583
SITE     1 BC2  4 PHE C  29  SER C  97  MPD C 302  HOH C 401
SITE     1 BC3  8 THR C  30  LEU C  96  SER C  97  GLU C 156
SITE     2 BC3  8 ALA C 158  MPD C 301  HOH C 401  HOH C 482
SITE     1 BC4  4 GLN C  86  ASP C 231  GLN C 234  PRO C 235
SITE     1 BC5  3 GLY C  47  TYR C  48  HOH C 519
SITE     1 BC6  6 PHE D  29  GLY D  31  GLU D 156  HIS D 226
SITE     2 BC6  6 MPD D 302  HOH D 493
SITE     1 BC7  6 SER D  97  ILE D 125  LEU D 170  VAL D 198
SITE     2 BC7  6 MPD D 301  HOH D 517
SITE     1 BC8  3 ASN D 151  ASP D 231  TYR D 232
SITE     1 BC9  8 PHE E  29  SER E  97  ILE E 125  LEU E 167
SITE     2 BC9  8 LEU E 170  VAL E 198  MPD E 302  HOH E 402
SITE     1 CC1  7 PHE E  29  THR E  30  LEU E  96  GLU E 156
SITE     2 CC1  7 VAL E 227  MPD E 301  HOH E 552
SITE     1 CC2 10 VAL E  21  ALA E  46  GLY E  47  TYR E  48
SITE     2 CC2 10 ALA E 249  HOH E 407  ALA F  46  TYR F  48
SITE     3 CC2 10 HOH F 402  HOH F 421
SITE     1 CC3  4 ASN E 151  ASP E 231  TYR E 232  GLN E 234
SITE     1 CC4  4 PHE F  29  SER F  97  VAL F 198  HOH F 462
SITE     1 CC5  6 PHE F  29  GLU F 156  ALA F 158  VAL F 227
SITE     2 CC5  6 HOH F 463  HOH F 504
SITE     1 CC6  5 ASN F 151  LEU F 230  ASP F 231  TYR F 232
SITE     2 CC6  5 GLN F 234
CRYST1   39.165  183.131  244.704  90.00  90.00  90.00 P 21 21 21   24
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.025533  0.000000  0.000000        0.00000
SCALE2      0.000000  0.005461  0.000000        0.00000
SCALE3      0.000000  0.000000  0.004087        0.00000
TER    1984      ALA A 249
TER    3906      GLY B 250
TER    5864      ALA C 249
TER    7783      ALA D 249
TER    9680      GLY E 250
TER   11566      GLY F 250
MASTER      622    0   24   65   54    0   38    612423    6  192  126
END