longtext: 4KRX-pdb

content
HEADER    HYDROLASE                               17-MAY-13   4KRX
TITLE     STRUCTURE OF AES FROM E. COLI
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ACETYL ESTERASE;
COMPND   3 CHAIN: A, B, C;
COMPND   4 SYNONYM: AES, ECE;
COMPND   5 EC: 3.1.1.-;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;
SOURCE   3 ORGANISM_TAXID: 83333;
SOURCE   4 STRAIN: K12;
SOURCE   5 GENE: AES, B0476, JW0465, YBAC;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: RD130;
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PQE31
KEYWDS    ALPHA/BETA-HYDROLASE, HORMONE-SENSITIVE-LIPASE FAMILY, INHIBITION OF
KEYWDS   2 MALT, ACYL ESTERASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    A.SCHIEFNER,K.GERBER,A.BROSIG,W.BOOS
REVDAT   1   21-AUG-13 4KRX    0
JRNL        AUTH   A.SCHIEFNER,K.GERBER,A.BROSIG,W.BOOS
JRNL        TITL   STRUCTURAL AND MUTATIONAL ANALYSES OF AES, AN INHIBITOR OF
JRNL        TITL 2 MALT IN ESCHERICHIA COLI.
JRNL        REF    PROTEINS                                   2013
JRNL        REFN                   ESSN 1097-0134
JRNL        PMID   23934774
JRNL        DOI    10.1002/PROT.24383
REMARK   2
REMARK   2 RESOLUTION.    1.80 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.7.0029
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.80
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 30.00
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.3
REMARK   3   NUMBER OF REFLECTIONS             : 104093
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.161
REMARK   3   R VALUE            (WORKING SET) : 0.161
REMARK   3   FREE R VALUE                     : 0.190
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 2.000
REMARK   3   FREE R VALUE TEST SET COUNT      : 2084
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.80
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.85
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 6839
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 91.42
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2750
REMARK   3   BIN FREE R VALUE SET COUNT          : 127
REMARK   3   BIN FREE R VALUE                    : 0.3150
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 7507
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 26
REMARK   3   SOLVENT ATOMS            : 658
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 32.08
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 28.99
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : -1.22000
REMARK   3    B22 (A**2) : -1.22000
REMARK   3    B33 (A**2) : 3.95000
REMARK   3    B12 (A**2) : -1.22000
REMARK   3    B13 (A**2) : -0.00000
REMARK   3    B23 (A**2) : -0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.101
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.098
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.075
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 5.000
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.973
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.962
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  7858 ; 0.019 ; 0.019
REMARK   3   BOND LENGTHS OTHERS               (A):  7263 ; 0.001 ; 0.020
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 10711 ; 1.858 ; 1.966
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 16685 ; 0.894 ; 3.001
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   974 ; 5.683 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   383 ;33.600 ;23.551
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1242 ;13.509 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    59 ;19.494 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1145 ; 0.111 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  9075 ; 0.010 ; 0.021
REMARK   3   GENERAL PLANES OTHERS             (A):  1900 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 3
REMARK   3
REMARK   3   TLS GROUP : 1
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A     4        A   319
REMARK   3    ORIGIN FOR THE GROUP (A): -37.1741 -24.6718  -0.1866
REMARK   3    T TENSOR
REMARK   3      T11:   0.0900 T22:   0.0811
REMARK   3      T33:   0.0221 T12:   0.0005
REMARK   3      T13:  -0.0138 T23:   0.0001
REMARK   3    L TENSOR
REMARK   3      L11:   0.3231 L22:   0.2383
REMARK   3      L33:   0.1515 L12:   0.0083
REMARK   3      L13:   0.0362 L23:  -0.1017
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0285 S12:  -0.0200 S13:   0.0712
REMARK   3      S21:   0.0147 S22:   0.0099 S23:  -0.0347
REMARK   3      S31:   0.0359 S32:  -0.0275 S33:   0.0186
REMARK   3
REMARK   3   TLS GROUP : 2
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B     4        B   319
REMARK   3    ORIGIN FOR THE GROUP (A): -73.1877 -46.1927   5.0186
REMARK   3    T TENSOR
REMARK   3      T11:   0.0798 T22:   0.0755
REMARK   3      T33:   0.0308 T12:   0.0059
REMARK   3      T13:  -0.0071 T23:  -0.0018
REMARK   3    L TENSOR
REMARK   3      L11:   0.3456 L22:   0.3469
REMARK   3      L33:   0.1387 L12:   0.1831
REMARK   3      L13:  -0.0733 L23:   0.1248
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0271 S12:  -0.0227 S13:   0.0386
REMARK   3      S21:  -0.0431 S22:  -0.0195 S23:   0.1010
REMARK   3      S31:  -0.0054 S32:   0.0337 S33:   0.0465
REMARK   3
REMARK   3   TLS GROUP : 3
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   C     7        C   319
REMARK   3    ORIGIN FOR THE GROUP (A): -73.1622 -84.5043  -3.0863
REMARK   3    T TENSOR
REMARK   3      T11:   0.0709 T22:   0.0699
REMARK   3      T33:   0.0381 T12:  -0.0072
REMARK   3      T13:   0.0127 T23:  -0.0028
REMARK   3    L TENSOR
REMARK   3      L11:   0.3728 L22:   0.6384
REMARK   3      L33:   0.0789 L12:  -0.1420
REMARK   3      L13:   0.0105 L23:  -0.0071
REMARK   3    S TENSOR
REMARK   3      S11:   0.0057 S12:   0.0384 S13:  -0.0498
REMARK   3      S21:   0.0465 S22:  -0.0045 S23:   0.1543
REMARK   3      S31:  -0.0037 S32:   0.0714 S33:  -0.0012
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS:
REMARK   3  HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
REMARK   3  U VALUES      : WITH TLS ADDED
REMARK   4
REMARK   4 4KRX COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 30-MAY-13.
REMARK 100 THE RCSB ID CODE IS RCSB079718.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 22-OCT-12
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 8.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SLS
REMARK 200  BEAMLINE                       : X06SA
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.99987
REMARK 200  MONOCHROMATOR                  : FIXED-EXIT LN2 COOLED DOUBLE
REMARK 200                                   CRYSTAL SI(111)
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : PSI PILATUS 6M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XSCALE
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 104093
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.800
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.3
REMARK 200  DATA REDUNDANCY                : 9.900
REMARK 200  R MERGE                    (I) : 0.07100
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 22.4300
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.80
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.90
REMARK 200  COMPLETENESS FOR SHELL     (%) : 95.5
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 0.80600
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 2.820
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: PDB ENTRY 3GA7
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 50.57
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.49
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M LITHIUM SULFATE, 0.1 M TRIS/HCL,
REMARK 280  30% W/V PEG4000, PH 8.5, VAPOR DIFFUSION, SITTING DROP,
REMARK 280  TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 32 2 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -Y,X-Y,Z+2/3
REMARK 290       3555   -X+Y,-X,Z+1/3
REMARK 290       4555   Y,X,-Z
REMARK 290       5555   X-Y,-Y,-Z+1/3
REMARK 290       6555   -X,-X+Y,-Z+2/3
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      100.66933
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       50.33467
REMARK 290   SMTRY1   4 -0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   4  0.866025  0.500000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   5  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       50.33467
REMARK 290   SMTRY1   6 -0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   6 -0.866025  0.500000  0.000000        0.00000
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000      100.66933
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 2990 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 24440 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: 4.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 1550 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 24710 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -8.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350   BIOMT1   2 -0.500000  0.866025  0.000000        0.00000
REMARK 350   BIOMT2   2  0.866025  0.500000  0.000000        0.00000
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH C 550  LIES ON A SPECIAL POSITION.
REMARK 375      HOH C 416  LIES ON A SPECIAL POSITION.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A   -13
REMARK 465     ARG A   -12
REMARK 465     GLY A   -11
REMARK 465     SER A   -10
REMARK 465     HIS A    -9
REMARK 465     HIS A    -8
REMARK 465     HIS A    -7
REMARK 465     HIS A    -6
REMARK 465     HIS A    -5
REMARK 465     HIS A    -4
REMARK 465     THR A    -3
REMARK 465     ASP A    -2
REMARK 465     PRO A    -1
REMARK 465     ILE A     0
REMARK 465     MET A     1
REMARK 465     LYS A     2
REMARK 465     PRO A     3
REMARK 465     MET B   -13
REMARK 465     ARG B   -12
REMARK 465     GLY B   -11
REMARK 465     SER B   -10
REMARK 465     HIS B    -9
REMARK 465     HIS B    -8
REMARK 465     HIS B    -7
REMARK 465     HIS B    -6
REMARK 465     HIS B    -5
REMARK 465     HIS B    -4
REMARK 465     THR B    -3
REMARK 465     ASP B    -2
REMARK 465     PRO B    -1
REMARK 465     ILE B     0
REMARK 465     MET B     1
REMARK 465     LYS B     2
REMARK 465     PRO B     3
REMARK 465     MET C   -13
REMARK 465     ARG C   -12
REMARK 465     GLY C   -11
REMARK 465     SER C   -10
REMARK 465     HIS C    -9
REMARK 465     HIS C    -8
REMARK 465     HIS C    -7
REMARK 465     HIS C    -6
REMARK 465     HIS C    -5
REMARK 465     HIS C    -4
REMARK 465     THR C    -3
REMARK 465     ASP C    -2
REMARK 465     PRO C    -1
REMARK 465     ILE C     0
REMARK 465     MET C     1
REMARK 465     LYS C     2
REMARK 465     PRO C     3
REMARK 465     GLU C     4
REMARK 465     ASN C     5
REMARK 465     LYS C     6
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    GLU C  16   CD    GLU C  16   OE2     0.079
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ASP A 184   CB  -  CG  -  OD1 ANGL. DEV. =   7.2 DEGREES
REMARK 500    ASP A 184   CB  -  CG  -  OD2 ANGL. DEV. =  -5.9 DEGREES
REMARK 500    ARG A 207   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.8 DEGREES
REMARK 500    ARG A 249   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES
REMARK 500    ARG B 105   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES
REMARK 500    ASP B 246   CB  -  CG  -  OD1 ANGL. DEV. =   5.8 DEGREES
REMARK 500    ARG B 269   NE  -  CZ  -  NH1 ANGL. DEV. =   3.3 DEGREES
REMARK 500    ARG C 269   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.5 DEGREES
REMARK 500    MET C 300   CG  -  SD  -  CE  ANGL. DEV. = -11.9 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PRO A  29      173.11    -59.38
REMARK 500    PHE A  95       -5.63     72.24
REMARK 500    SER A 165     -123.71     55.53
REMARK 500    TYR A 195       57.44     37.73
REMARK 500    TYR A 240      -62.03   -120.02
REMARK 500    PHE B  95      -12.26     78.32
REMARK 500    ASN B  99     -171.98   -170.21
REMARK 500    SER B 165     -124.78     58.22
REMARK 500    PRO B 238        0.15    -65.32
REMARK 500    PHE B 261       42.77   -107.04
REMARK 500    HIS B 292      151.62    -46.67
REMARK 500    ASP C  82       60.74   -118.08
REMARK 500    PHE C  95      -12.99     72.37
REMARK 500    SER C 165     -123.56     60.35
REMARK 500    TYR C 195       52.59     36.76
REMARK 500    TYR C 240      -62.44   -120.67
REMARK 500    PHE C 261       53.61   -105.10
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PG4 A 401
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PG4 B 401
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4KRY   RELATED DB: PDB
DBREF  4KRX A    1   319  UNP    P23872   AES_ECOLI        1    319
DBREF  4KRX B    1   319  UNP    P23872   AES_ECOLI        1    319
DBREF  4KRX C    1   319  UNP    P23872   AES_ECOLI        1    319
SEQADV 4KRX MET A  -13  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ARG A  -12  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX GLY A  -11  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX SER A  -10  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS A   -9  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS A   -8  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS A   -7  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS A   -6  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS A   -5  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS A   -4  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX THR A   -3  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ASP A   -2  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX PRO A   -1  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ILE A    0  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX MET B  -13  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ARG B  -12  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX GLY B  -11  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX SER B  -10  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS B   -9  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS B   -8  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS B   -7  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS B   -6  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS B   -5  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS B   -4  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX THR B   -3  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ASP B   -2  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX PRO B   -1  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ILE B    0  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX MET C  -13  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ARG C  -12  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX GLY C  -11  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX SER C  -10  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS C   -9  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS C   -8  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS C   -7  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS C   -6  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS C   -5  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX HIS C   -4  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX THR C   -3  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ASP C   -2  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX PRO C   -1  UNP  P23872              EXPRESSION TAG
SEQADV 4KRX ILE C    0  UNP  P23872              EXPRESSION TAG
SEQRES   1 A  333  MET ARG GLY SER HIS HIS HIS HIS HIS HIS THR ASP PRO
SEQRES   2 A  333  ILE MET LYS PRO GLU ASN LYS LEU PRO VAL LEU ASP LEU
SEQRES   3 A  333  ILE SER ALA GLU MET LYS THR VAL VAL ASN THR LEU GLN
SEQRES   4 A  333  PRO ASP LEU PRO PRO TRP PRO ALA THR GLY THR ILE ALA
SEQRES   5 A  333  GLU GLN ARG GLN TYR TYR THR LEU GLU ARG ARG PHE TRP
SEQRES   6 A  333  ASN ALA GLY ALA PRO GLU MET ALA THR ARG ALA TYR MET
SEQRES   7 A  333  VAL PRO THR LYS TYR GLY GLN VAL GLU THR ARG LEU PHE
SEQRES   8 A  333  CYS PRO GLN PRO ASP SER PRO ALA THR LEU PHE TYR LEU
SEQRES   9 A  333  HIS GLY GLY GLY PHE ILE LEU GLY ASN LEU ASP THR HIS
SEQRES  10 A  333  ASP ARG ILE MET ARG LEU LEU ALA SER TYR SER GLN CYS
SEQRES  11 A  333  THR VAL ILE GLY ILE ASP TYR THR LEU SER PRO GLU ALA
SEQRES  12 A  333  ARG PHE PRO GLN ALA ILE GLU GLU ILE VAL ALA ALA CYS
SEQRES  13 A  333  CYS TYR PHE HIS GLN GLN ALA GLU ASP TYR GLN ILE ASN
SEQRES  14 A  333  MET SER ARG ILE GLY PHE ALA GLY ASP SER ALA GLY ALA
SEQRES  15 A  333  MET LEU ALA LEU ALA SER ALA LEU TRP LEU ARG ASP LYS
SEQRES  16 A  333  GLN ILE ASP CYS GLY LYS VAL ALA GLY VAL LEU LEU TRP
SEQRES  17 A  333  TYR GLY LEU TYR GLY LEU ARG ASP SER VAL THR ARG ARG
SEQRES  18 A  333  LEU LEU GLY GLY VAL TRP ASP GLY LEU THR GLN GLN ASP
SEQRES  19 A  333  LEU GLN MET TYR GLU GLU ALA TYR LEU SER ASN ASP ALA
SEQRES  20 A  333  ASP ARG GLU SER PRO TYR TYR CYS LEU PHE ASN ASN ASP
SEQRES  21 A  333  LEU THR ARG GLU VAL PRO PRO CYS PHE ILE ALA GLY ALA
SEQRES  22 A  333  GLU PHE ASP PRO LEU LEU ASP ASP SER ARG LEU LEU TYR
SEQRES  23 A  333  GLN THR LEU ALA ALA HIS GLN GLN PRO CYS GLU PHE LYS
SEQRES  24 A  333  LEU TYR PRO GLY THR LEU HIS ALA PHE LEU HIS TYR SER
SEQRES  25 A  333  ARG MET MET LYS THR ALA ASP GLU ALA LEU ARG ASP GLY
SEQRES  26 A  333  ALA GLN PHE PHE THR ALA GLN LEU
SEQRES   1 B  333  MET ARG GLY SER HIS HIS HIS HIS HIS HIS THR ASP PRO
SEQRES   2 B  333  ILE MET LYS PRO GLU ASN LYS LEU PRO VAL LEU ASP LEU
SEQRES   3 B  333  ILE SER ALA GLU MET LYS THR VAL VAL ASN THR LEU GLN
SEQRES   4 B  333  PRO ASP LEU PRO PRO TRP PRO ALA THR GLY THR ILE ALA
SEQRES   5 B  333  GLU GLN ARG GLN TYR TYR THR LEU GLU ARG ARG PHE TRP
SEQRES   6 B  333  ASN ALA GLY ALA PRO GLU MET ALA THR ARG ALA TYR MET
SEQRES   7 B  333  VAL PRO THR LYS TYR GLY GLN VAL GLU THR ARG LEU PHE
SEQRES   8 B  333  CYS PRO GLN PRO ASP SER PRO ALA THR LEU PHE TYR LEU
SEQRES   9 B  333  HIS GLY GLY GLY PHE ILE LEU GLY ASN LEU ASP THR HIS
SEQRES  10 B  333  ASP ARG ILE MET ARG LEU LEU ALA SER TYR SER GLN CYS
SEQRES  11 B  333  THR VAL ILE GLY ILE ASP TYR THR LEU SER PRO GLU ALA
SEQRES  12 B  333  ARG PHE PRO GLN ALA ILE GLU GLU ILE VAL ALA ALA CYS
SEQRES  13 B  333  CYS TYR PHE HIS GLN GLN ALA GLU ASP TYR GLN ILE ASN
SEQRES  14 B  333  MET SER ARG ILE GLY PHE ALA GLY ASP SER ALA GLY ALA
SEQRES  15 B  333  MET LEU ALA LEU ALA SER ALA LEU TRP LEU ARG ASP LYS
SEQRES  16 B  333  GLN ILE ASP CYS GLY LYS VAL ALA GLY VAL LEU LEU TRP
SEQRES  17 B  333  TYR GLY LEU TYR GLY LEU ARG ASP SER VAL THR ARG ARG
SEQRES  18 B  333  LEU LEU GLY GLY VAL TRP ASP GLY LEU THR GLN GLN ASP
SEQRES  19 B  333  LEU GLN MET TYR GLU GLU ALA TYR LEU SER ASN ASP ALA
SEQRES  20 B  333  ASP ARG GLU SER PRO TYR TYR CYS LEU PHE ASN ASN ASP
SEQRES  21 B  333  LEU THR ARG GLU VAL PRO PRO CYS PHE ILE ALA GLY ALA
SEQRES  22 B  333  GLU PHE ASP PRO LEU LEU ASP ASP SER ARG LEU LEU TYR
SEQRES  23 B  333  GLN THR LEU ALA ALA HIS GLN GLN PRO CYS GLU PHE LYS
SEQRES  24 B  333  LEU TYR PRO GLY THR LEU HIS ALA PHE LEU HIS TYR SER
SEQRES  25 B  333  ARG MET MET LYS THR ALA ASP GLU ALA LEU ARG ASP GLY
SEQRES  26 B  333  ALA GLN PHE PHE THR ALA GLN LEU
SEQRES   1 C  333  MET ARG GLY SER HIS HIS HIS HIS HIS HIS THR ASP PRO
SEQRES   2 C  333  ILE MET LYS PRO GLU ASN LYS LEU PRO VAL LEU ASP LEU
SEQRES   3 C  333  ILE SER ALA GLU MET LYS THR VAL VAL ASN THR LEU GLN
SEQRES   4 C  333  PRO ASP LEU PRO PRO TRP PRO ALA THR GLY THR ILE ALA
SEQRES   5 C  333  GLU GLN ARG GLN TYR TYR THR LEU GLU ARG ARG PHE TRP
SEQRES   6 C  333  ASN ALA GLY ALA PRO GLU MET ALA THR ARG ALA TYR MET
SEQRES   7 C  333  VAL PRO THR LYS TYR GLY GLN VAL GLU THR ARG LEU PHE
SEQRES   8 C  333  CYS PRO GLN PRO ASP SER PRO ALA THR LEU PHE TYR LEU
SEQRES   9 C  333  HIS GLY GLY GLY PHE ILE LEU GLY ASN LEU ASP THR HIS
SEQRES  10 C  333  ASP ARG ILE MET ARG LEU LEU ALA SER TYR SER GLN CYS
SEQRES  11 C  333  THR VAL ILE GLY ILE ASP TYR THR LEU SER PRO GLU ALA
SEQRES  12 C  333  ARG PHE PRO GLN ALA ILE GLU GLU ILE VAL ALA ALA CYS
SEQRES  13 C  333  CYS TYR PHE HIS GLN GLN ALA GLU ASP TYR GLN ILE ASN
SEQRES  14 C  333  MET SER ARG ILE GLY PHE ALA GLY ASP SER ALA GLY ALA
SEQRES  15 C  333  MET LEU ALA LEU ALA SER ALA LEU TRP LEU ARG ASP LYS
SEQRES  16 C  333  GLN ILE ASP CYS GLY LYS VAL ALA GLY VAL LEU LEU TRP
SEQRES  17 C  333  TYR GLY LEU TYR GLY LEU ARG ASP SER VAL THR ARG ARG
SEQRES  18 C  333  LEU LEU GLY GLY VAL TRP ASP GLY LEU THR GLN GLN ASP
SEQRES  19 C  333  LEU GLN MET TYR GLU GLU ALA TYR LEU SER ASN ASP ALA
SEQRES  20 C  333  ASP ARG GLU SER PRO TYR TYR CYS LEU PHE ASN ASN ASP
SEQRES  21 C  333  LEU THR ARG GLU VAL PRO PRO CYS PHE ILE ALA GLY ALA
SEQRES  22 C  333  GLU PHE ASP PRO LEU LEU ASP ASP SER ARG LEU LEU TYR
SEQRES  23 C  333  GLN THR LEU ALA ALA HIS GLN GLN PRO CYS GLU PHE LYS
SEQRES  24 C  333  LEU TYR PRO GLY THR LEU HIS ALA PHE LEU HIS TYR SER
SEQRES  25 C  333  ARG MET MET LYS THR ALA ASP GLU ALA LEU ARG ASP GLY
SEQRES  26 C  333  ALA GLN PHE PHE THR ALA GLN LEU
HET    PG4  A 401      13
HET    PG4  B 401      13
HETNAM     PG4 TETRAETHYLENE GLYCOL
FORMUL   4  PG4    2(C8 H18 O5)
FORMUL   6  HOH   *658(H2 O)
HELIX    1   1 PRO A    8  ILE A   13  1                                   6
HELIX    2   2 SER A   14  ASN A   22  1                                   9
HELIX    3   3 THR A   36  ALA A   53  1                                  18
HELIX    4   4 HIS A  103  GLN A  115  1                                  13
HELIX    5   5 PRO A  132  GLN A  148  1                                  17
HELIX    6   6 GLN A  148  GLN A  153  1                                   6
HELIX    7   7 SER A  165  LYS A  181  1                                  17
HELIX    8   8 SER A  203  LEU A  209  1                                   7
HELIX    9   9 THR A  217  LEU A  229  1                                  13
HELIX   10  10 ASN A  231  SER A  237  5                                   7
HELIX   11  11 CYS A  241  ASN A  245  5                                   5
HELIX   12  12 LEU A  264  HIS A  278  1                                  15
HELIX   13  13 ALA A  293  SER A  298  5                                   6
HELIX   14  14 MET A  301  GLN A  318  1                                  18
HELIX   15  15 PRO B    8  ILE B   13  1                                   6
HELIX   16  16 SER B   14  THR B   23  1                                  10
HELIX   17  17 THR B   36  ALA B   53  1                                  18
HELIX   18  18 HIS B  103  GLN B  115  1                                  13
HELIX   19  19 PRO B  132  GLN B  148  1                                  17
HELIX   20  20 GLN B  148  GLN B  153  1                                   6
HELIX   21  21 SER B  165  LYS B  181  1                                  17
HELIX   22  22 SER B  203  LEU B  209  1                                   7
HELIX   23  23 THR B  217  LEU B  229  1                                  13
HELIX   24  24 ASN B  231  SER B  237  5                                   7
HELIX   25  25 CYS B  241  ASN B  245  5                                   5
HELIX   26  26 LEU B  264  HIS B  278  1                                  15
HELIX   27  27 ALA B  293  SER B  298  5                                   6
HELIX   28  28 MET B  301  GLN B  318  1                                  18
HELIX   29  29 PRO C    8  ILE C   13  1                                   6
HELIX   30  30 SER C   14  ASN C   22  1                                   9
HELIX   31  31 THR C   36  ALA C   53  1                                  18
HELIX   32  32 HIS C  103  GLN C  115  1                                  13
HELIX   33  33 PRO C  132  GLN C  148  1                                  17
HELIX   34  34 GLN C  148  GLN C  153  1                                   6
HELIX   35  35 SER C  165  LYS C  181  1                                  17
HELIX   36  36 SER C  203  LEU C  209  1                                   7
HELIX   37  37 THR C  217  LEU C  229  1                                  13
HELIX   38  38 ASN C  231  SER C  237  5                                   7
HELIX   39  39 CYS C  241  ASN C  245  5                                   5
HELIX   40  40 LEU C  264  HIS C  278  1                                  15
HELIX   41  41 ALA C  293  SER C  298  5                                   6
HELIX   42  42 MET C  301  GLN C  318  1                                  18
SHEET    1   A 8 ALA A  59  VAL A  65  0
SHEET    2   A 8 VAL A  72  CYS A  78 -1  O  LEU A  76   N  ARG A  61
SHEET    3   A 8 THR A 117  ILE A 121 -1  O  VAL A 118   N  PHE A  77
SHEET    4   A 8 THR A  86  LEU A  90  1  N  LEU A  87   O  ILE A 119
SHEET    5   A 8 ARG A 158  ASP A 164  1  O  GLY A 160   N  PHE A  88
SHEET    6   A 8 LYS A 187  TRP A 194  1  O  ALA A 189   N  ILE A 159
SHEET    7   A 8 CYS A 254  ALA A 259  1  O  PHE A 255   N  LEU A 193
SHEET    8   A 8 CYS A 282  TYR A 287  1  O  GLU A 283   N  ILE A 256
SHEET    1   B 8 ALA B  59  VAL B  65  0
SHEET    2   B 8 VAL B  72  CYS B  78 -1  O  CYS B  78   N  ALA B  59
SHEET    3   B 8 THR B 117  ILE B 121 -1  O  VAL B 118   N  PHE B  77
SHEET    4   B 8 THR B  86  LEU B  90  1  N  LEU B  87   O  THR B 117
SHEET    5   B 8 ARG B 158  ASP B 164  1  O  ALA B 162   N  LEU B  90
SHEET    6   B 8 LYS B 187  TRP B 194  1  O  ALA B 189   N  ILE B 159
SHEET    7   B 8 CYS B 254  ALA B 259  1  O  PHE B 255   N  LEU B 193
SHEET    8   B 8 CYS B 282  TYR B 287  1  O  GLU B 283   N  ILE B 256
SHEET    1   C 8 ALA C  59  VAL C  65  0
SHEET    2   C 8 VAL C  72  CYS C  78 -1  O  LEU C  76   N  ARG C  61
SHEET    3   C 8 THR C 117  ILE C 121 -1  O  VAL C 118   N  PHE C  77
SHEET    4   C 8 THR C  86  LEU C  90  1  N  LEU C  87   O  THR C 117
SHEET    5   C 8 ARG C 158  ASP C 164  1  O  ALA C 162   N  LEU C  90
SHEET    6   C 8 LYS C 187  TRP C 194  1  O  ALA C 189   N  ILE C 159
SHEET    7   C 8 CYS C 254  ALA C 259  1  O  ALA C 257   N  LEU C 193
SHEET    8   C 8 CYS C 282  TYR C 287  1  O  GLU C 283   N  ILE C 256
SSBOND   1 CYS A  143    CYS A  185                          1555   1555  2.04
SSBOND   2 CYS B  143    CYS B  185                          1555   1555  2.04
SSBOND   3 CYS C  143    CYS C  185                          1555   1555  2.06
CISPEP   1 SER A  126    PRO A  127          0         8.00
CISPEP   2 PHE A  131    PRO A  132          0        -2.32
CISPEP   3 SER B  126    PRO B  127          0         2.46
CISPEP   4 PHE B  131    PRO B  132          0        -0.15
CISPEP   5 SER C  126    PRO C  127          0         3.35
CISPEP   6 PHE C  131    PRO C  132          0         3.25
SITE     1 AC1 12 GLY A  93  GLY A  94  SER A 165  ALA A 166
SITE     2 AC1 12 LEU A 197  LEU A 216  TYR A 224  HOH A 553
SITE     3 AC1 12 HOH A 582  HOH A 698  HOH A 702  HOH A 737
SITE     1 AC2 10 GLY B  93  GLY B  94  PHE B  95  SER B 165
SITE     2 AC2 10 LEU B 197  LEU B 216  TYR B 224  HOH B 538
SITE     3 AC2 10 HOH B 637  HOH B 659
CRYST1  113.712  113.712  151.004  90.00  90.00 120.00 P 32 2 1     18
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.008794  0.005077  0.000000        0.00000
SCALE2      0.000000  0.010155  0.000000        0.00000
SCALE3      0.000000  0.000000  0.006622        0.00000
TER    2547      LEU A 319
TER    5090      LEU B 319
TER    7623      LEU C 319
MASTER      480    0    2   42   24    0    6    6 8191    3   32   78
END