longtext: 4QE3-pdb

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HEADER    TRANSFERASE                             15-MAY-14   4QE3
TITLE     CRYSTAL STRUCTURE OF ANTIGEN 85C-H260Q MUTANT
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: DIACYLGLYCEROL ACYLTRANSFERASE/MYCOLYLTRANSFERASE AG85C;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: DGAT, ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE, ANTIGEN 85
COMPND   5 COMPLEX C, 85C, AG85C, FIBRONECTIN-BINDING PROTEIN C, FBPS C;
COMPND   6 EC: 2.3.1.122, 2.3.1.20;
COMPND   7 ENGINEERED: YES;
COMPND   8 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: MYCOBACTERIUM TUBERCULOSIS;
SOURCE   3 ORGANISM_TAXID: 83332;
SOURCE   4 STRAIN: H37RV;
SOURCE   5 GENE: FBPC, MPT45, MTCI5.03C, RV0129C;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    MYCOLYLTRANSFERASE/DIACYLGLYCEROL ACYLTRANSFERASE, TRANSFERASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    L.FAVROT,D.H.LAJINESS,D.R.RONNING
REVDAT   1   09-JUL-14 4QE3    0
JRNL        AUTH   L.FAVROT,D.H.LAJINESS,D.R.RONNING
JRNL        TITL   INACTIVATION OF THE MYCOBACTERIUM TUBERCULOSIS ANTIGEN 85
JRNL        TITL 2 COMPLEX BY COVALENT, ALLOSTERIC INHIBITORS.
JRNL        REF    J.BIOL.CHEM.                               2014
JRNL        REFN                   ESSN 1083-351X
REMARK   2
REMARK   2 RESOLUTION.    1.35 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.9_1692)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER
REMARK   3               : ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.35
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 38.15
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.330
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.7
REMARK   3   NUMBER OF REFLECTIONS             : 68398
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.173
REMARK   3   R VALUE            (WORKING SET) : 0.171
REMARK   3   FREE R VALUE                     : 0.198
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.990
REMARK   3   FREE R VALUE TEST SET COUNT      : 3413
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 38.1687 -  3.8951    0.98     2920   156  0.1580 0.1894
REMARK   3     2  3.8951 -  3.0921    0.97     2757   147  0.1679 0.2059
REMARK   3     3  3.0921 -  2.7013    1.00     2807   147  0.1763 0.1943
REMARK   3     4  2.7013 -  2.4544    1.00     2819   149  0.1794 0.2213
REMARK   3     5  2.4544 -  2.2785    1.00     2779   146  0.1695 0.2058
REMARK   3     6  2.2785 -  2.1442    0.96     2687   140  0.1679 0.2009
REMARK   3     7  2.1442 -  2.0368    1.00     2781   146  0.1656 0.1726
REMARK   3     8  2.0368 -  1.9481    1.00     2759   145  0.1686 0.1884
REMARK   3     9  1.9481 -  1.8731    0.99     2762   145  0.1689 0.1791
REMARK   3    10  1.8731 -  1.8085    1.00     2755   146  0.1669 0.1866
REMARK   3    11  1.8085 -  1.7520    1.00     2749   144  0.1684 0.2195
REMARK   3    12  1.7520 -  1.7019    1.00     2753   145  0.1720 0.1949
REMARK   3    13  1.7019 -  1.6571    1.00     2752   145  0.1596 0.1769
REMARK   3    14  1.6571 -  1.6167    1.00     2762   145  0.1606 0.1849
REMARK   3    15  1.6167 -  1.5799    1.00     2728   144  0.1612 0.1722
REMARK   3    16  1.5799 -  1.5463    1.00     2715   143  0.1622 0.2013
REMARK   3    17  1.5463 -  1.5153    1.00     2784   145  0.1703 0.2244
REMARK   3    18  1.5153 -  1.4867    1.00     2726   144  0.1790 0.1818
REMARK   3    19  1.4867 -  1.4602    1.00     2737   145  0.1803 0.2020
REMARK   3    20  1.4602 -  1.4354    0.99     2723   143  0.1895 0.1899
REMARK   3    21  1.4354 -  1.4123    0.98     2670   142  0.2087 0.2295
REMARK   3    22  1.4123 -  1.3906    0.93     2546   137  0.2183 0.2435
REMARK   3    23  1.3906 -  1.3701    0.87     2396   116  0.2327 0.2732
REMARK   3    24  1.3701 -  1.3508    0.78     2118   108  0.2512 0.2228
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.120
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 18.040
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.006           2199
REMARK   3   ANGLE     :  1.115           3014
REMARK   3   CHIRALITY :  0.048            308
REMARK   3   PLANARITY :  0.007            394
REMARK   3   DIHEDRAL  : 13.466            767
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 4QE3 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 22-MAY-14.
REMARK 100 THE RCSB ID CODE IS RCSB085938.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 30-JUL-13
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 4.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : APS
REMARK 200  BEAMLINE                       : 23-ID-D
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97936
REMARK 200  MONOCHROMATOR                  : DOUBLE CRYSTAL MONOCHROMATOR
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 300 MM CCD
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : NULL
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.350
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.9
REMARK 200  DATA REDUNDANCY                : 5.500
REMARK 200  R MERGE                    (I) : 0.07800
REMARK 200  R SYM                      (I) : 0.07800
REMARK 200   FOR THE DATA SET  : 16.0370
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.35
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.40
REMARK 200  COMPLETENESS FOR SHELL     (%) : 85.5
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.90
REMARK 200  R MERGE FOR SHELL          (I) : 0.51300
REMARK 200  R SYM FOR SHELL            (I) : 0.51300
REMARK 200   FOR SHELL         : 2.814
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: EPMR
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 48.47
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.39
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 20% PEG 3350, 0.1M SODIUM ACETATE
REMARK 280  TRIHYDRATE, 6MM CHAPSO, PH 4.5, VAPOR DIFFUSION, HANGING DROP,
REMARK 280  TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       30.36250
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       38.15350
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       34.06400
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       38.15350
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       30.36250
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       34.06400
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A    -1
REMARK 465     ALA A     0
REMARK 465     PHE A     1
REMARK 465     SER A     2
REMARK 465     ARG A     3
REMARK 465     PRO A     4
REMARK 465     GLY A     5
REMARK 465     GLY A   212
REMARK 465     THR A   213
REMARK 465     PRO A   214
REMARK 465     SER A   215
REMARK 465     ASP A   216
REMARK 465     LEU A   217
REMARK 465     GLY A   218
REMARK 465     GLY A   219
REMARK 465     ASP A   220
REMARK 465     ASN A   221
REMARK 465     ALA A   283
REMARK 465     THR A   284
REMARK 465     PRO A   285
REMARK 465     PRO A   286
REMARK 465     ALA A   287
REMARK 465     ALA A   288
REMARK 465     PRO A   289
REMARK 465     ALA A   290
REMARK 465     ALA A   291
REMARK 465     PRO A   292
REMARK 465     ALA A   293
REMARK 465     ALA A   294
REMARK 465     HIS A   295
REMARK 465     HIS A   296
REMARK 465     HIS A   297
REMARK 465     HIS A   298
REMARK 465     HIS A   299
REMARK 465     HIS A   300
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PRO A   7       40.26    -76.34
REMARK 500    PRO A  54       35.15    -91.91
REMARK 500    PHE A  76       18.57     54.50
REMARK 500    GLN A  85       66.61    -58.66
REMARK 500    SER A  86      -75.30    175.33
REMARK 500    ARG A 101      -63.94   -129.19
REMARK 500    SER A 124     -123.85     48.48
REMARK 500    ASN A 152       58.66   -142.10
REMARK 500    SER A 169       75.21   -100.44
REMARK 500    ARG A 248       16.51   -141.54
REMARK 500
REMARK 500 REMARK: NULL
REMARK 610
REMARK 610 MISSING HETEROATOM
REMARK 610 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 610 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 610 I=INSERTION CODE):
REMARK 610   M RES C SSEQI
REMARK 610     1N7 A  401
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE 1N7 A 401
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ACY A 402
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4QDO   RELATED DB: PDB
REMARK 900 RELATED ID: 4QDT   RELATED DB: PDB
REMARK 900 RELATED ID: 4QDU   RELATED DB: PDB
REMARK 900 RELATED ID: 4QDX   RELATED DB: PDB
REMARK 900 RELATED ID: 4QDZ   RELATED DB: PDB
REMARK 900 RELATED ID: 4QEK   RELATED DB: PDB
DBREF  4QE3 A    0   294  UNP    P9WQN9   A85C_MYCTU      46    340
SEQADV 4QE3 MET A   -1  UNP  P9WQN9              EXPRESSION TAG
SEQADV 4QE3 GLN A  260  UNP  P9WQN9    HIS   306 ENGINEERED MUTATION
SEQADV 4QE3 HIS A  295  UNP  P9WQN9              EXPRESSION TAG
SEQADV 4QE3 HIS A  296  UNP  P9WQN9              EXPRESSION TAG
SEQADV 4QE3 HIS A  297  UNP  P9WQN9              EXPRESSION TAG
SEQADV 4QE3 HIS A  298  UNP  P9WQN9              EXPRESSION TAG
SEQADV 4QE3 HIS A  299  UNP  P9WQN9              EXPRESSION TAG
SEQADV 4QE3 HIS A  300  UNP  P9WQN9              EXPRESSION TAG
SEQRES   1 A  302  MET ALA PHE SER ARG PRO GLY LEU PRO VAL GLU TYR LEU
SEQRES   2 A  302  GLN VAL PRO SER ALA SER MET GLY ARG ASP ILE LYS VAL
SEQRES   3 A  302  GLN PHE GLN GLY GLY GLY PRO HIS ALA VAL TYR LEU LEU
SEQRES   4 A  302  ASP GLY LEU ARG ALA GLN ASP ASP TYR ASN GLY TRP ASP
SEQRES   5 A  302  ILE ASN THR PRO ALA PHE GLU GLU TYR TYR GLN SER GLY
SEQRES   6 A  302  LEU SER VAL ILE MET PRO VAL GLY GLY GLN SER SER PHE
SEQRES   7 A  302  TYR THR ASP TRP TYR GLN PRO SER GLN SER ASN GLY GLN
SEQRES   8 A  302  ASN TYR THR TYR LYS TRP GLU THR PHE LEU THR ARG GLU
SEQRES   9 A  302  MET PRO ALA TRP LEU GLN ALA ASN LYS GLY VAL SER PRO
SEQRES  10 A  302  THR GLY ASN ALA ALA VAL GLY LEU SER MET SER GLY GLY
SEQRES  11 A  302  SER ALA LEU ILE LEU ALA ALA TYR TYR PRO GLN GLN PHE
SEQRES  12 A  302  PRO TYR ALA ALA SER LEU SER GLY PHE LEU ASN PRO SER
SEQRES  13 A  302  GLU GLY TRP TRP PRO THR LEU ILE GLY LEU ALA MET ASN
SEQRES  14 A  302  ASP SER GLY GLY TYR ASN ALA ASN SER MET TRP GLY PRO
SEQRES  15 A  302  SER SER ASP PRO ALA TRP LYS ARG ASN ASP PRO MET VAL
SEQRES  16 A  302  GLN ILE PRO ARG LEU VAL ALA ASN ASN THR ARG ILE TRP
SEQRES  17 A  302  VAL TYR CYS GLY ASN GLY THR PRO SER ASP LEU GLY GLY
SEQRES  18 A  302  ASP ASN ILE PRO ALA LYS PHE LEU GLU GLY LEU THR LEU
SEQRES  19 A  302  ARG THR ASN GLN THR PHE ARG ASP THR TYR ALA ALA ASP
SEQRES  20 A  302  GLY GLY ARG ASN GLY VAL PHE ASN PHE PRO PRO ASN GLY
SEQRES  21 A  302  THR GLN SER TRP PRO TYR TRP ASN GLU GLN LEU VAL ALA
SEQRES  22 A  302  MET LYS ALA ASP ILE GLN HIS VAL LEU ASN GLY ALA THR
SEQRES  23 A  302  PRO PRO ALA ALA PRO ALA ALA PRO ALA ALA HIS HIS HIS
SEQRES  24 A  302  HIS HIS HIS
HET    1N7  A 401      26
HET    ACY  A 402       4
HETNAM     1N7 CHAPSO
HETNAM     ACY ACETIC ACID
HETSYN     1N7 2-HYDROXY-N,N-DIMETHYL-3-SULFO-N-(3-{[(3BETA,5BETA,
HETSYN   2 1N7  7BETA,12BETA)-3,7,12-TRIHYDROXY-24-OXOCHOLAN-24-
HETSYN   3 1N7  YL]AMINO}PROPYL)PROPAN-1-AMINIUM
FORMUL   2  1N7    C32 H59 N2 O8 S 1+
FORMUL   3  ACY    C2 H4 O2
FORMUL   4  HOH   *234(H2 O)
HELIX    1   1 ASN A   47  THR A   53  1                                   7
HELIX    2   2 PRO A   54  TYR A   60  1                                   7
HELIX    3   3 LYS A   94  ARG A  101  1                                   8
HELIX    4   4 ARG A  101  GLY A  112  1                                  12
HELIX    5   5 SER A  124  TYR A  137  1                                  14
HELIX    6   6 TRP A  157  SER A  169  1                                  13
HELIX    7   7 ASN A  173  GLY A  179  1                                   7
HELIX    8   8 ASP A  183  ASN A  189  1                                   7
HELIX    9   9 GLN A  194  ASN A  201  1                                   8
HELIX   10  10 PRO A  223  ASP A  245  1                                  23
HELIX   11  11 SER A  261  MET A  272  1                                  12
HELIX   12  12 MET A  272  GLY A  282  1                                  11
SHEET    1   A 8 GLU A   9  SER A  15  0
SHEET    2   A 8 ARG A  20  GLN A  27 -1  O  PHE A  26   N  GLU A   9
SHEET    3   A 8 SER A  65  PRO A  69 -1  O  MET A  68   N  GLN A  25
SHEET    4   A 8 ALA A  33  LEU A  36  1  N  LEU A  36   O  ILE A  67
SHEET    5   A 8 ALA A 119  LEU A 123  1  O  ALA A 119   N  TYR A  35
SHEET    6   A 8 TYR A 143  LEU A 147  1  O  ALA A 145   N  ALA A 120
SHEET    7   A 8 ARG A 204  TYR A 208  1  O  TYR A 208   N  SER A 146
SHEET    8   A 8 GLY A 250  ASN A 253  1  O  VAL A 251   N  VAL A 207
SITE     1 AC1 10 THR A 100  ARG A 101  GLN A 108  GLN A 140
SITE     2 AC1 10 ASN A 167  GLY A 170  GLY A 171  HOH A 555
SITE     3 AC1 10 HOH A 581  HOH A 613
SITE     1 AC2  2 ASP A  50  HOH A 708
CRYST1   60.725   68.128   76.307  90.00  90.00  90.00 P 21 21 21    4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.016468  0.000000  0.000000        0.00000
SCALE2      0.000000  0.014678  0.000000        0.00000
SCALE3      0.000000  0.000000  0.013105        0.00000
TER    2098      GLY A 282
MASTER      307    0    2   12    8    0    4    6 2355    1   30   24
END