longtext: 4WFI-pdb

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HEADER    HYDROLASE                               15-SEP-14   4WFI
TITLE     CRYSTAL STRUCRURE OF PET-DEGRADING CUTINASE CUT190 S226P MUTANT IN
TITLE    2 CA(2+)-FREE STATE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: CUTINASE;
COMPND   3 CHAIN: A;
COMPND   4 FRAGMENT: UNP RESIDUES 47-304;
COMPND   5 ENGINEERED: YES;
COMPND   6 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: SACCHAROMONOSPORA VIRIDIS;
SOURCE   3 ORGANISM_TAXID: 1852;
SOURCE   4 STRAIN: AHK190;
SOURCE   5 GENE: CUT190;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: ROSETTA-GAMI B (DE3)
KEYWDS    CUTINASE, POLYESTERASE, ALPHA/BETA-HYDROLASE FOLD
EXPDTA    X-RAY DIFFRACTION
AUTHOR    T.MIYAKAWA,H.MIZUSHIMA,J.OHTSUKA,M.ODA,F.KAWAI,M.TANOKURA
REVDAT   1   24-DEC-14 4WFI    0
JRNL        AUTH   T.MIYAKAWA,H.MIZUSHIMA,J.OHTSUKA,M.ODA,F.KAWAI,M.TANOKURA
JRNL        TITL   STRUCTURAL BASIS FOR THE CA(2+)-ENHANCED THERMOSTABILITY AND
JRNL        TITL 2 ACTIVITY OF PET-DEGRADING CUTINASE-LIKE ENZYME FROM
JRNL        TITL 3 SACCHAROMONOSPORA VIRIDIS AHK190.
JRNL        REF    APPL.MICROBIOL.BIOTECHNOL.                 2014
JRNL        REFN                   ESSN 1432-0614
JRNL        PMID   25492421
JRNL        DOI    10.1007/S00253-014-6272-8
REMARK   2
REMARK   2 RESOLUTION.    1.45 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.9_1692)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.45
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.50
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 0.000
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.7
REMARK   3   NUMBER OF REFLECTIONS             : 47174
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.176
REMARK   3   R VALUE            (WORKING SET) : 0.176
REMARK   3   FREE R VALUE                     : 0.191
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.050
REMARK   3   FREE R VALUE TEST SET COUNT      : 2381
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 19.4994 -  3.7104    0.99     2825   142  0.1681 0.1749
REMARK   3     2  3.7104 -  2.9483    1.00     2727   131  0.1763 0.1848
REMARK   3     3  2.9483 -  2.5766    1.00     2646   156  0.1748 0.1864
REMARK   3     4  2.5766 -  2.3414    1.00     2652   162  0.1701 0.1941
REMARK   3     5  2.3414 -  2.1738    1.00     2648   128  0.1667 0.1824
REMARK   3     6  2.1738 -  2.0458    1.00     2670   124  0.1721 0.1819
REMARK   3     7  2.0458 -  1.9435    1.00     2626   145  0.1684 0.2058
REMARK   3     8  1.9435 -  1.8589    1.00     2618   144  0.1724 0.1971
REMARK   3     9  1.8589 -  1.7874    1.00     2631   136  0.1726 0.1952
REMARK   3    10  1.7874 -  1.7258    1.00     2622   150  0.1751 0.2234
REMARK   3    11  1.7258 -  1.6718    1.00     2590   142  0.1724 0.1794
REMARK   3    12  1.6718 -  1.6241    1.00     2631   144  0.1702 0.2067
REMARK   3    13  1.6241 -  1.5813    1.00     2601   123  0.1790 0.2132
REMARK   3    14  1.5813 -  1.5428    1.00     2633   139  0.1832 0.2322
REMARK   3    15  1.5428 -  1.5077    1.00     2579   147  0.1861 0.2260
REMARK   3    16  1.5077 -  1.4756    1.00     2593   139  0.1842 0.2436
REMARK   3    17  1.4756 -  1.4461    0.95     2501   129  0.2003 0.2357
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.130
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 18.580
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.006           2065
REMARK   3   ANGLE     :  1.069           2811
REMARK   3   CHIRALITY :  0.044            302
REMARK   3   PLANARITY :  0.006            372
REMARK   3   DIHEDRAL  : 10.894            761
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 4WFI COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 02-OCT-14.
REMARK 100 THE DEPOSITION ID IS D_1000203639.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 02-DEC-12
REMARK 200  TEMPERATURE           (KELVIN) : 95
REMARK 200  PH                             : 7.5
REMARK 200  NUMBER OF CRYSTALS USED        : NULL
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : PHOTON FACTORY
REMARK 200  BEAMLINE                       : BL-17A
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.000
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 270
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : NULL
REMARK 200  DATA SCALING SOFTWARE          : NULL
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 47285
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.446
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0
REMARK 200  DATA REDUNDANCY                : 7.200
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 74.7000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: NULL
REMARK 200 SOFTWARE USED: NULL
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 42.77
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.15
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 50 MM BIS-TRIS, 50 MM AMMONIUM
REMARK 280  SULFATE, 25% (W/V) PENTAERYTHRITOL ETHOXYLATE (15/4 EO/OH)
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       28.72350
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       34.78750
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       32.57100
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       34.78750
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       28.72350
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       32.57100
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A    35
REMARK 465     ARG A    36
REMARK 465     GLY A    37
REMARK 465     SER A    38
REMARK 465     HIS A    39
REMARK 465     HIS A    40
REMARK 465     HIS A    41
REMARK 465     HIS A    42
REMARK 465     HIS A    43
REMARK 465     HIS A    44
REMARK 465     GLY A    45
REMARK 465     SER A    46
REMARK 465     LYS A   305
REMARK 465     LEU A   306
REMARK 465     ASN A   307
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    HOH A   559     O    HOH A   616              1.98
REMARK 500   ND2  ASN A   168     O    HOH A   401              2.10
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A 176     -122.86     65.37
REMARK 500    THR A 199       59.31     30.96
REMARK 500    HIS A 230      -84.59   -122.94
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4WFJ   RELATED DB: PDB
REMARK 900 RELATED ID: 4WFK   RELATED DB: PDB
DBREF  4WFI A   47   304  UNP    W0TJ64   W0TJ64_9PSEU    47    304
SEQADV 4WFI MET A   35  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI ARG A   36  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI GLY A   37  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI SER A   38  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI HIS A   39  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI HIS A   40  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI HIS A   41  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI HIS A   42  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI HIS A   43  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI HIS A   44  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI GLY A   45  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI SER A   46  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI PRO A  226  UNP  W0TJ64    SER   226 ENGINEERED MUTATION
SEQADV 4WFI LYS A  305  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI LEU A  306  UNP  W0TJ64              EXPRESSION TAG
SEQADV 4WFI ASN A  307  UNP  W0TJ64              EXPRESSION TAG
SEQRES   1 A  273  MET ARG GLY SER HIS HIS HIS HIS HIS HIS GLY SER ASN
SEQRES   2 A  273  PRO TYR GLU ARG GLY PRO ASP PRO THR GLU ASP SER ILE
SEQRES   3 A  273  GLU ALA ILE ARG GLY PRO PHE SER VAL ALA THR GLU ARG
SEQRES   4 A  273  VAL SER SER PHE ALA SER GLY PHE GLY GLY GLY THR ILE
SEQRES   5 A  273  TYR TYR PRO ARG GLU THR ASP GLU GLY THR PHE GLY ALA
SEQRES   6 A  273  VAL ALA VAL ALA PRO GLY PHE THR ALA SER GLN GLY SER
SEQRES   7 A  273  MET SER TRP TYR GLY GLU ARG VAL ALA SER GLN GLY PHE
SEQRES   8 A  273  ILE VAL PHE THR ILE ASP THR ASN THR ARG LEU ASP GLN
SEQRES   9 A  273  PRO GLY GLN ARG GLY ARG GLN LEU LEU ALA ALA LEU ASP
SEQRES  10 A  273  TYR LEU VAL GLU ARG SER ASP ARG LYS VAL ARG GLU ARG
SEQRES  11 A  273  LEU ASP PRO ASN ARG LEU ALA VAL MET GLY HIS SER MET
SEQRES  12 A  273  GLY GLY GLY GLY SER LEU GLU ALA THR VAL MET ARG PRO
SEQRES  13 A  273  SER LEU LYS ALA SER ILE PRO LEU THR PRO TRP ASN LEU
SEQRES  14 A  273  ASP LYS THR TRP GLY GLN VAL GLN VAL PRO THR PHE ILE
SEQRES  15 A  273  ILE GLY ALA GLU LEU ASP THR ILE ALA PRO VAL ARG THR
SEQRES  16 A  273  HIS ALA LYS PRO PHE TYR GLU SER LEU PRO SER SER LEU
SEQRES  17 A  273  PRO LYS ALA TYR MET GLU LEU ASP GLY ALA THR HIS PHE
SEQRES  18 A  273  ALA PRO ASN ILE PRO ASN THR THR ILE ALA LYS TYR VAL
SEQRES  19 A  273  ILE SER TRP LEU LYS ARG PHE VAL ASP GLU ASP THR ARG
SEQRES  20 A  273  TYR SER GLN PHE LEU CYS PRO ASN PRO THR ASP ARG ALA
SEQRES  21 A  273  ILE GLU GLU TYR ARG SER THR CYS PRO TYR LYS LEU ASN
FORMUL   2  HOH   *226(H2 O)
HELIX    1 AA1 THR A   56  ALA A   62  1                                   7
HELIX    2 AA2 ALA A  108  SER A  112  5                                   5
HELIX    3 AA3 MET A  113  SER A  114  5                                   2
HELIX    4 AA4 TRP A  115  SER A  122  1                                   8
HELIX    5 AA5 GLN A  138  ARG A  156  1                                  19
HELIX    6 AA6 ASP A  158  GLU A  163  1                                   6
HELIX    7 AA7 SER A  176  ARG A  189  1                                  14
HELIX    8 AA8 HIS A  230  LEU A  238  1                                   9
HELIX    9 AA9 PHE A  255  ILE A  259  5                                   5
HELIX   10 AB1 ASN A  261  ASP A  277  1                                  17
HELIX   11 AB2 ASP A  279  ARG A  281  5                                   3
HELIX   12 AB3 TYR A  282  CYS A  287  1                                   6
SHEET    1 AA1 6 VAL A  69  ALA A  78  0
SHEET    2 AA1 6 GLY A  82  PRO A  89 -1  O  ILE A  86   N  GLU A  72
SHEET    3 AA1 6 ILE A 126  ILE A 130 -1  O  VAL A 127   N  TYR A  87
SHEET    4 AA1 6 PHE A  97  ALA A 103  1  N  VAL A 102   O  PHE A 128
SHEET    5 AA1 6 LEU A 165  HIS A 175  1  O  ASP A 166   N  PHE A  97
SHEET    6 AA1 6 ALA A 194  LEU A 198  1  O  LEU A 198   N  GLY A 174
SHEET    1 AA2 3 THR A 214  ALA A 219  0
SHEET    2 AA2 3 LYS A 244  LEU A 249  1  O  LEU A 249   N  GLY A 218
SHEET    3 AA2 3 ILE A 295  SER A 300 -1  O  GLU A 297   N  GLU A 248
SSBOND   1 CYS A  287    CYS A  302                          1555   1555  2.03
CISPEP   1 CYS A  287    PRO A  288          0        -3.28
CISPEP   2 CYS A  302    PRO A  303          0        -4.13
CRYST1   57.447   65.142   69.575  90.00  90.00  90.00 P 21 21 21    4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.017407  0.000000  0.000000        0.00000
SCALE2      0.000000  0.015351  0.000000        0.00000
SCALE3      0.000000  0.000000  0.014373        0.00000
TER    2011      TYR A 304
MASTER      260    0    0   12    9    0    0    6 2236    1    2   21
END