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HEADER HYDROLASE 09-DEC-14 4X71
TITLE CRYSTAL STRUCTURE OF LIPASE FROM GEOBACILLUS STEAROTHERMOPHILUS T6
TITLE 2 METHANOL STABLE VARIANT A269T
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: LIPASE;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: UNP RESIDUES 33-418;
COMPND 5 EC: 3.1.1.3;
COMPND 6 ENGINEERED: YES;
COMPND 7 MUTATION: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: GEOBACILLUS STEAROTHERMOPHILUS T6;
SOURCE 3 ORGANISM_TAXID: 646309;
SOURCE 4 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE 5 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE 6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 7 EXPRESSION_SYSTEM_PLASMID: PET9A
KEYWDS HYDROLASE
EXPDTA X-RAY DIFFRACTION
AUTHOR M.KANTEEV,A.DROR,S.GIHAZ,A.SHAHAR,A.FISHMAN
REVDAT 1 10-JUN-15 4X71 0
JRNL AUTH A.DROR,M.KANTEEV,I.KAGAN,S.GIHAZ,A.SHAHAR,A.FISHMAN
JRNL TITL STRUCTURAL INSIGHTS INTO METHANOL-STABLE VARIANTS OF LIPASE
JRNL TITL 2 T6 FROM GEOBACILLUS STEAROTHERMOPHILUS.
JRNL REF APPL.MICROBIOL.BIOTECHNOL. 2015
JRNL REFN ESSN 1432-0614
JRNL PMID 26026940
JRNL DOI 10.1007/S00253-015-6700-4
REMARK 2
REMARK 2 RESOLUTION. 2.00 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : PHENIX
REMARK 3 AUTHORS : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK 3 : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK 3 : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK 3 : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK 3 : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK 3 : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK 3 : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK 3 : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK 3
REMARK 3 REFINEMENT TARGET : ML
REMARK 3
REMARK 3 DATA USED IN REFINEMENT.
REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS) : 2.00
REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS) : 37.40
REMARK 3 MIN(FOBS/SIGMA_FOBS) : 1.340
REMARK 3 COMPLETENESS FOR RANGE (%) : 100.0
REMARK 3 NUMBER OF REFLECTIONS : 27809
REMARK 3
REMARK 3 FIT TO DATA USED IN REFINEMENT.
REMARK 3 R VALUE (WORKING + TEST SET) : 0.192
REMARK 3 R VALUE (WORKING SET) : 0.191
REMARK 3 FREE R VALUE : 0.205
REMARK 3 FREE R VALUE TEST SET SIZE (%) : 5.030
REMARK 3 FREE R VALUE TEST SET COUNT : 1399
REMARK 3
REMARK 3 FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK 3 BIN RESOLUTION RANGE COMPL. NWORK NFREE RWORK RFREE
REMARK 3 1 37.4077 - 4.3070 1.00 2826 137 0.1819 0.1957
REMARK 3 2 4.3070 - 3.4193 1.00 2677 157 0.1619 0.1655
REMARK 3 3 3.4193 - 2.9873 1.00 2619 156 0.1977 0.1900
REMARK 3 4 2.9873 - 2.7142 1.00 2624 150 0.2083 0.2139
REMARK 3 5 2.7142 - 2.5197 1.00 2640 123 0.2064 0.2308
REMARK 3 6 2.5197 - 2.3712 1.00 2615 146 0.2011 0.2338
REMARK 3 7 2.3712 - 2.2525 1.00 2618 110 0.1949 0.2427
REMARK 3 8 2.2525 - 2.1544 1.00 2610 133 0.1980 0.2067
REMARK 3 9 2.1544 - 2.0715 1.00 2583 145 0.1963 0.2435
REMARK 3 10 2.0715 - 2.0000 1.00 2598 142 0.2176 0.2549
REMARK 3
REMARK 3 BULK SOLVENT MODELLING.
REMARK 3 METHOD USED : FLAT BULK SOLVENT MODEL
REMARK 3 SOLVENT RADIUS : 1.20
REMARK 3 SHRINKAGE RADIUS : 0.98
REMARK 3 K_SOL : 0.36
REMARK 3 B_SOL : 30.84
REMARK 3
REMARK 3 ERROR ESTIMATES.
REMARK 3 COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED) : 0.180
REMARK 3 PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 20.300
REMARK 3
REMARK 3 B VALUES.
REMARK 3 FROM WILSON PLOT (A**2) : NULL
REMARK 3 MEAN B VALUE (OVERALL, A**2) : 20.49
REMARK 3 OVERALL ANISOTROPIC B VALUE.
REMARK 3 B11 (A**2) : -6.03490
REMARK 3 B22 (A**2) : -2.22630
REMARK 3 B33 (A**2) : 8.26120
REMARK 3 B12 (A**2) : 0.00000
REMARK 3 B13 (A**2) : 0.00000
REMARK 3 B23 (A**2) : 0.00000
REMARK 3
REMARK 3 TWINNING INFORMATION.
REMARK 3 FRACTION: NULL
REMARK 3 OPERATOR: NULL
REMARK 3
REMARK 3 DEVIATIONS FROM IDEAL VALUES.
REMARK 3 RMSD COUNT
REMARK 3 BOND : 0.020 3211
REMARK 3 ANGLE : 1.287 4374
REMARK 3 CHIRALITY : 0.107 453
REMARK 3 PLANARITY : 0.005 571
REMARK 3 DIHEDRAL : 13.155 1135
REMARK 3
REMARK 3 TLS DETAILS
REMARK 3 NUMBER OF TLS GROUPS : 1
REMARK 3 TLS GROUP : 1
REMARK 3 SELECTION: ALL
REMARK 3 ORIGIN FOR THE GROUP (A): 10.5475 10.4752 18.4104
REMARK 3 T TENSOR
REMARK 3 T11: 0.0888 T22: 0.0934
REMARK 3 T33: 0.0868 T12: 0.0071
REMARK 3 T13: 0.0044 T23: 0.0041
REMARK 3 L TENSOR
REMARK 3 L11: 0.1905 L22: 0.2163
REMARK 3 L33: 0.1802 L12: 0.0068
REMARK 3 L13: 0.0229 L23: -0.0498
REMARK 3 S TENSOR
REMARK 3 S11: -0.0048 S12: 0.0097 S13: -0.0075
REMARK 3 S21: 0.0052 S22: -0.0150 S23: 0.0563
REMARK 3 S31: -0.0054 S32: -0.0141 S33: -0.0489
REMARK 3
REMARK 3 NCS DETAILS
REMARK 3 NUMBER OF NCS GROUPS : NULL
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 4X71 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 09-DEC-14.
REMARK 100 THE DEPOSITION ID IS D_1000205202.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200 EXPERIMENT TYPE : X-RAY DIFFRACTION
REMARK 200 DATE OF DATA COLLECTION : 13-JUL-13
REMARK 200 TEMPERATURE (KELVIN) : 100
REMARK 200 PH : NULL
REMARK 200 NUMBER OF CRYSTALS USED : 1
REMARK 200
REMARK 200 SYNCHROTRON (Y/N) : Y
REMARK 200 RADIATION SOURCE : ESRF
REMARK 200 BEAMLINE : BM14
REMARK 200 X-RAY GENERATOR MODEL : NULL
REMARK 200 MONOCHROMATIC OR LAUE (M/L) : M
REMARK 200 WAVELENGTH OR RANGE (A) : 0.7
REMARK 200 MONOCHROMATOR : NULL
REMARK 200 OPTICS : NULL
REMARK 200
REMARK 200 DETECTOR TYPE : CCD
REMARK 200 DETECTOR MANUFACTURER : MARMOSAIC 225 MM CCD
REMARK 200 INTENSITY-INTEGRATION SOFTWARE : NULL
REMARK 200 DATA SCALING SOFTWARE : SCALA 3.3.20
REMARK 200
REMARK 200 NUMBER OF UNIQUE REFLECTIONS : 27872
REMARK 200 RESOLUTION RANGE HIGH (A) : 2.000
REMARK 200 RESOLUTION RANGE LOW (A) : 60.121
REMARK 200 REJECTION CRITERIA (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200 COMPLETENESS FOR RANGE (%) : 100.0
REMARK 200 DATA REDUNDANCY : 10.80
REMARK 200 R MERGE (I) : NULL
REMARK 200 R SYM (I) : 0.08600
REMARK 200 FOR THE DATA SET : 20.6000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 2.11
REMARK 200 COMPLETENESS FOR SHELL (%) : 100.0
REMARK 200 DATA REDUNDANCY IN SHELL : 10.70
REMARK 200 R MERGE FOR SHELL (I) : 0.17600
REMARK 200 R SYM FOR SHELL (I) : 0.17600
REMARK 200 FOR SHELL : 4.100
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER 2.3.0
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS (%): 45.87
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.27
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2M SODIUM CITRATE, 25% PEG3350,
REMARK 280 VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290 SYMOP SYMMETRY
REMARK 290 NNNMMM OPERATOR
REMARK 290 1555 X,Y,Z
REMARK 290 2555 -X+1/2,-Y,Z+1/2
REMARK 290 3555 -X,Y+1/2,-Z+1/2
REMARK 290 4555 X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290 WHERE NNN -> OPERATOR NUMBER
REMARK 290 MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000
REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000
REMARK 290 SMTRY1 2 -1.000000 0.000000 0.000000 24.70500
REMARK 290 SMTRY2 2 0.000000 -1.000000 0.000000 0.00000
REMARK 290 SMTRY3 2 0.000000 0.000000 1.000000 57.23500
REMARK 290 SMTRY1 3 -1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 3 0.000000 1.000000 0.000000 35.32500
REMARK 290 SMTRY3 3 0.000000 0.000000 -1.000000 57.23500
REMARK 290 SMTRY1 4 1.000000 0.000000 0.000000 24.70500
REMARK 290 SMTRY2 4 0.000000 -1.000000 0.000000 35.32500
REMARK 290 SMTRY3 4 0.000000 0.000000 -1.000000 0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 300 REMARK: BIOLOGICAL UNIT IS THE SAME AS ASYM.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 90 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 15330 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -12.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 SER A 114 -137.84 58.16
REMARK 500 ASP A 176 32.29 -99.97
REMARK 500 VAL A 204 -59.24 66.67
REMARK 500 LEU A 209 42.36 -93.51
REMARK 500 ARG A 272 38.67 -150.55
REMARK 500 ASN A 305 97.25 -160.48
REMARK 500 ASP A 311 -159.73 -110.29
REMARK 500 ILE A 320 -37.20 -132.72
REMARK 500 LYS A 330 -49.64 -136.49
REMARK 500 ASN A 368 87.30 -164.10
REMARK 500
REMARK 500 REMARK: NULL
REMARK 620
REMARK 620 METAL COORDINATION
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 620
REMARK 620 COORDINATION ANGLES FOR: M RES CSSEQI METAL
REMARK 620 ZN A 401 ZN
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 ASP A 62 OD1
REMARK 620 2 ASP A 62 OD2 54.4
REMARK 620 3 HIS A 82 NE2 96.8 150.7
REMARK 620 4 HIS A 88 NE2 117.3 90.6 100.0
REMARK 620 5 ASP A 239 OD2 138.3 100.4 106.3 92.6
REMARK 620 N 1 2 3 4
REMARK 620
REMARK 620 COORDINATION ANGLES FOR: M RES CSSEQI METAL
REMARK 620 CA A 402 CA
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 GLY A 287 O
REMARK 620 2 GLU A 361 OE2 69.6
REMARK 620 3 ASP A 366 OD2 121.3 119.9
REMARK 620 4 PRO A 367 O 147.6 91.5 90.8
REMARK 620 5 HOH A 811 O 71.3 81.0 157.7 80.2
REMARK 620 6 HOH A 673 O 98.2 165.3 73.1 95.4 87.4
REMARK 620 N 1 2 3 4 5
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue ZN A 401
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue CA A 402
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4X6U RELATED DB: PDB
DBREF 4X71 A 4 389 UNP Q93A71 Q93A71_GEOSE 33 418
SEQADV 4X71 THR A 269 UNP Q93A71 ALA 298 ENGINEERED MUTATION
SEQADV 4X71 ALA A 323 UNP Q93A71 THR 352 CONFLICT
SEQADV 4X71 HIS A 390 UNP Q93A71 EXPRESSION TAG
SEQADV 4X71 HIS A 391 UNP Q93A71 EXPRESSION TAG
SEQADV 4X71 HIS A 392 UNP Q93A71 EXPRESSION TAG
SEQADV 4X71 HIS A 393 UNP Q93A71 EXPRESSION TAG
SEQADV 4X71 HIS A 394 UNP Q93A71 EXPRESSION TAG
SEQADV 4X71 HIS A 395 UNP Q93A71 EXPRESSION TAG
SEQRES 1 A 392 SER ARG ALA ASN ASP ALA PRO ILE VAL LEU LEU HIS GLY
SEQRES 2 A 392 PHE THR GLY TRP GLY ARG GLU GLU MET PHE GLY PHE LYS
SEQRES 3 A 392 TYR TRP GLY GLY VAL ARG GLY ASP ILE GLU GLN TRP LEU
SEQRES 4 A 392 ASN ASP ASN GLY TYR ARG THR TYR THR LEU ALA VAL GLY
SEQRES 5 A 392 PRO LEU SER SER ASN TRP ASP ARG ALA CYS GLU ALA TYR
SEQRES 6 A 392 ALA GLN LEU VAL GLY GLY THR VAL ASP TYR GLY ALA ALA
SEQRES 7 A 392 HIS ALA ALA LYS HIS GLY HIS ALA ARG PHE GLY ARG THR
SEQRES 8 A 392 TYR PRO GLY LEU LEU PRO GLU LEU LYS ARG GLY GLY ARG
SEQRES 9 A 392 ILE HIS ILE ILE ALA HIS SER GLN GLY GLY GLN THR ALA
SEQRES 10 A 392 ARG MET LEU VAL SER LEU LEU GLU ASN GLY SER GLN GLU
SEQRES 11 A 392 GLU ARG GLU TYR ALA LYS ALA HIS ASN VAL SER LEU SER
SEQRES 12 A 392 PRO LEU PHE GLU GLY GLY HIS HIS PHE VAL LEU SER VAL
SEQRES 13 A 392 THR THR ILE ALA THR PRO HIS ASP GLY THR THR LEU VAL
SEQRES 14 A 392 ASN MET VAL ASP PHE THR ASP ARG PHE PHE ASP LEU GLN
SEQRES 15 A 392 LYS ALA VAL LEU LYS ALA ALA ALA VAL ALA SER ASN VAL
SEQRES 16 A 392 PRO TYR THR SER GLN VAL TYR ASP PHE LYS LEU ASP GLN
SEQRES 17 A 392 TRP GLY LEU ARG ARG GLN PRO GLY GLU SER PHE ASP GLN
SEQRES 18 A 392 TYR PHE GLU ARG LEU LYS ARG SER PRO VAL TRP THR SER
SEQRES 19 A 392 THR ASP THR ALA ARG TYR ASP LEU SER VAL PRO GLY ALA
SEQRES 20 A 392 GLU LYS LEU ASN GLN TRP VAL LYS ALA SER PRO ASN THR
SEQRES 21 A 392 TYR TYR LEU SER PHE THR THR GLU ARG THR TYR ARG GLY
SEQRES 22 A 392 ALA LEU THR GLY ASN TYR TYR PRO GLU LEU GLY MET ASN
SEQRES 23 A 392 ALA PHE SER ALA VAL VAL CYS ALA PRO PHE LEU GLY SER
SEQRES 24 A 392 TYR ARG ASN ALA THR LEU GLY ILE ASP ASP ARG TRP LEU
SEQRES 25 A 392 GLU ASN ASP GLY ILE VAL ASN ALA PHE SER MET ASN GLY
SEQRES 26 A 392 PRO LYS ARG GLY SER THR ASP ARG ILE VAL PRO TYR ASP
SEQRES 27 A 392 GLY THR ILE LYS LYS GLY VAL TRP ASN ASP MET GLY THR
SEQRES 28 A 392 TYR ASN VAL ASP HIS LEU GLU VAL ILE GLY VAL ASP PRO
SEQRES 29 A 392 ASN PRO LEU PHE ASP ILE ARG ALA PHE TYR LEU ARG LEU
SEQRES 30 A 392 ALA GLU GLN LEU ALA SER LEU GLN PRO HIS HIS HIS HIS
SEQRES 31 A 392 HIS HIS
HET ZN A 401 1
HET CA A 402 1
HETNAM ZN ZINC ION
HETNAM CA CALCIUM ION
FORMUL 2 ZN ZN 2+
FORMUL 3 CA CA 2+
FORMUL 4 HOH *322(H2 O)
HELIX 1 AA1 GLU A 24 PHE A 28 5 5
HELIX 2 AA2 GLY A 32 GLY A 36 5 5
HELIX 3 AA3 ASP A 37 ASN A 45 1 9
HELIX 4 AA4 SER A 59 GLY A 73 1 15
HELIX 5 AA5 GLY A 79 GLY A 87 1 9
HELIX 6 AA6 LEU A 99 GLY A 105 5 7
HELIX 7 AA7 GLN A 115 GLY A 130 1 16
HELIX 8 AA8 SER A 131 ASN A 142 1 12
HELIX 9 AA9 SER A 146 GLU A 150 5 5
HELIX 10 AB1 THR A 169 MET A 174 5 6
HELIX 11 AB2 ASP A 176 ALA A 192 1 17
HELIX 12 AB3 SER A 221 LYS A 230 1 10
HELIX 13 AB4 SER A 232 SER A 237 1 6
HELIX 14 AB5 THR A 240 SER A 246 1 7
HELIX 15 AB6 SER A 246 GLN A 255 1 10
HELIX 16 AB7 ASN A 289 CYS A 296 1 8
HELIX 17 AB8 CYS A 296 GLY A 301 1 6
HELIX 18 AB9 ASP A 311 LEU A 315 5 5
HELIX 19 AC1 ASN A 322 MET A 326 5 5
HELIX 20 AC2 ASP A 372 SER A 386 1 15
SHEET 1 AA1 7 THR A 49 LEU A 52 0
SHEET 2 AA1 7 ILE A 11 LEU A 14 1 N ILE A 11 O TYR A 50
SHEET 3 AA1 7 ILE A 108 HIS A 113 1 O HIS A 109 N VAL A 12
SHEET 4 AA1 7 VAL A 156 ILE A 162 1 O THR A 160 N ALA A 112
SHEET 5 AA1 7 TYR A 264 THR A 270 1 O LEU A 266 N THR A 161
SHEET 6 AA1 7 TRP A 349 TYR A 355 1 O TYR A 355 N THR A 269
SHEET 7 AA1 7 ILE A 337 PRO A 339 1 N VAL A 338 O TRP A 349
SHEET 1 AA2 2 GLY A 74 ASP A 77 0
SHEET 2 AA2 2 PHE A 91 TYR A 95 -1 O TYR A 95 N GLY A 74
SHEET 1 AA3 2 THR A 273 ARG A 275 0
SHEET 2 AA3 2 TYR A 282 PRO A 284 -1 O TYR A 283 N TYR A 274
LINK OD1 ASP A 62 ZN ZN A 401 1555 1555 2.00
LINK OD2 ASP A 62 ZN ZN A 401 1555 1555 2.65
LINK NE2 HIS A 82 ZN ZN A 401 1555 1555 1.93
LINK NE2 HIS A 88 ZN ZN A 401 1555 1555 2.13
LINK OD2 ASP A 239 ZN ZN A 401 1555 1555 2.02
LINK O GLY A 287 CA CA A 402 1555 1555 2.20
LINK OE2 GLU A 361 CA CA A 402 1555 1555 2.28
LINK OD2 ASP A 366 CA CA A 402 1555 1555 2.27
LINK O PRO A 367 CA CA A 402 1555 1555 2.46
LINK CA CA A 402 O HOH A 811 1555 1555 2.91
LINK CA CA A 402 O HOH A 673 1555 1555 2.73
SITE 1 AC1 4 ASP A 62 HIS A 82 HIS A 88 ASP A 239
SITE 1 AC2 6 GLY A 287 GLU A 361 ASP A 366 PRO A 367
SITE 2 AC2 6 HOH A 673 HOH A 811
CRYST1 49.410 70.650 114.470 90.00 90.00 90.00 P 21 21 21 4
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 0.020239 0.000000 0.000000 0.00000
SCALE2 0.000000 0.014154 0.000000 0.00000
SCALE3 0.000000 0.000000 0.008736 0.00000
TER 3110 HIS A 395
MASTER 281 0 2 20 11 0 3 6 3433 1 15 31
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