longtext: 5jrk-pdb

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HEADER    HYDROLASE                               06-MAY-16   5JRK
TITLE     CRYSTAL STRUCTURE OF THE SPHINGOPYXIN I LASSO PEPTIDE ISOPEPTIDASE
TITLE    2 SPI-ISOP (SEMET-DERIVED)
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: DIPEPTIDYL AMINOPEPTIDASES/ACYLAMINOACYL-PEPTIDASES-LIKE
COMPND   3 PROTEIN;
COMPND   4 CHAIN: A, B;
COMPND   5 SYNONYM: SPI-ISOP;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: SPHINGOPYXIS ALASKENSIS RB2256;
SOURCE   3 ORGANISM_TAXID: 317655;
SOURCE   4 GENE: SALA_2532;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI B;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 37762;
SOURCE   7 EXPRESSION_SYSTEM_VARIANT: B834(DE3);
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   9 EXPRESSION_SYSTEM_VECTOR: PET28A
KEYWDS    LASSO PEPTIDE ISOPEPTIDASE, SERINE PROTEASE, BETA-PROPELLER,
KEYWDS   2 ALPHA/BETA-HYDROLASE, CATALYTIC TRIAD, OXYANION HOLE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    C.D.FAGE,J.D.HEGEMANN,G.BANGE,M.A.MARAHIEL
REVDAT   1   28-SEP-16 5JRK    0
JRNL        AUTH   C.D.FAGE,J.D.HEGEMANN,A.J.NEBEL,R.M.STEINBACH,S.ZHU,U.LINNE,
JRNL        AUTH 2 K.HARMS,G.BANGEM,M.A.MARAHIEL
JRNL        TITL   CRYSTAL STRUCTURE AND CHARACTERIZATION OF SPI-ISOP, AN
JRNL        TITL 2 ISOPEPTIDASE THAT HYDROLYZES THE LASSO PEPTIDE SPHINGOPYXIN
JRNL        TITL 3 I
JRNL        REF    ANGEW.CHEM.INT.ED.ENGL.                    2016
JRNL        REFN                   ESSN 1521-3773
JRNL        DOI    10.1002/ANIE.201605232
REMARK   2
REMARK   2 RESOLUTION.    3.00 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.10_2152
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.00
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 49.08
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340
REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.0
REMARK   3   NUMBER OF REFLECTIONS             : 51018
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.238
REMARK   3   R VALUE            (WORKING SET) : 0.237
REMARK   3   FREE R VALUE                     : 0.259
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.190
REMARK   3   FREE R VALUE TEST SET COUNT      : 2647
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 49.0870 -  7.9944    1.00     2706   165  0.2153 0.2323
REMARK   3     2  7.9944 -  6.3497    1.00     2631   129  0.2242 0.2091
REMARK   3     3  6.3497 -  5.5483    1.00     2608   121  0.2235 0.2630
REMARK   3     4  5.5483 -  5.0416    1.00     2569   138  0.2128 0.2303
REMARK   3     5  5.0416 -  4.6805    1.00     2547   144  0.1898 0.1874
REMARK   3     6  4.6805 -  4.4048    1.00     2561   132  0.2007 0.1944
REMARK   3     7  4.4048 -  4.1843    1.00     2543   141  0.2062 0.2212
REMARK   3     8  4.1843 -  4.0022    1.00     2536   138  0.2152 0.2427
REMARK   3     9  4.0022 -  3.8482    1.00     2532   130  0.2322 0.3023
REMARK   3    10  3.8482 -  3.7155    1.00     2530   128  0.2508 0.3140
REMARK   3    11  3.7155 -  3.5993    1.00     2513   151  0.2777 0.2942
REMARK   3    12  3.5993 -  3.4965    1.00     2537   144  0.2707 0.2807
REMARK   3    13  3.4965 -  3.4045    1.00     2501   150  0.2861 0.3080
REMARK   3    14  3.4045 -  3.3214    1.00     2502   134  0.3008 0.3808
REMARK   3    15  3.3214 -  3.2459    1.00     2510   152  0.3221 0.3573
REMARK   3    16  3.2459 -  3.1769    1.00     2504   140  0.3373 0.3844
REMARK   3    17  3.1769 -  3.1133    1.00     2522   141  0.3424 0.3650
REMARK   3    18  3.1133 -  3.0546    1.00     2540   127  0.3671 0.4103
REMARK   3    19  3.0546 -  3.0000    1.00     2479   142  0.3847 0.4375
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : NULL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.450
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 28.220
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 89.21
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 79.43
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.003          10918
REMARK   3   ANGLE     :  0.543          14828
REMARK   3   CHIRALITY :  0.043           1619
REMARK   3   PLANARITY :  0.003           1954
REMARK   3   DIHEDRAL  : 15.276           6553
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 5JRK COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 06-MAY-16.
REMARK 100 THE DEPOSITION ID IS D_1000221066.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 14-DEC-15
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 9.0
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : ESRF
REMARK 200  BEAMLINE                       : ID23-1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9790
REMARK 200  MONOCHROMATOR                  : SI (111)
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M-F
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS OCT 15, 2015
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS 0.5.17
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 51096
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.000
REMARK 200  RESOLUTION RANGE LOW       (A) : 49.080
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0
REMARK 200  DATA REDUNDANCY                : 13.30
REMARK 200  R MERGE                    (I) : 0.21900
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 12.9000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.00
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.09
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 27.80
REMARK 200  R MERGE FOR SHELL          (I) : 2.78000
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: SAD
REMARK 200 SOFTWARE USED: PHENIX 1.10-2152
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: POLYHEDRAL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 69.69
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 4.06
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M BICINE, 10%(W/V) PEG 6000, PH
REMARK 280  9.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291.15K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       55.55250
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       84.53500
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       66.75050
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       84.53500
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       55.55250
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       66.75050
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 4870 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 56110 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -20.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MSE A   -19
REMARK 465     GLY A   -18
REMARK 465     SER A   -17
REMARK 465     SER A   -16
REMARK 465     HIS A   -15
REMARK 465     HIS A   -14
REMARK 465     HIS A   -13
REMARK 465     HIS A   -12
REMARK 465     HIS A   -11
REMARK 465     HIS A   -10
REMARK 465     SER A    -9
REMARK 465     SER A    -8
REMARK 465     GLY A    -7
REMARK 465     LEU A    -6
REMARK 465     VAL A    -5
REMARK 465     PRO A    -4
REMARK 465     ARG A    -3
REMARK 465     GLY A    -2
REMARK 465     SER A    -1
REMARK 465     HIS A     0
REMARK 465     MSE A     1
REMARK 465     LYS A     2
REMARK 465     ILE A     3
REMARK 465     ALA A     4
REMARK 465     LEU A     5
REMARK 465     ARG A     6
REMARK 465     SER A     7
REMARK 465     GLY A     8
REMARK 465     LEU A     9
REMARK 465     PRO A    10
REMARK 465     ALA A    11
REMARK 465     VAL A    12
REMARK 465     ALA A    13
REMARK 465     LEU A    14
REMARK 465     LEU A    15
REMARK 465     ALA A    16
REMARK 465     ALA A    17
REMARK 465     ALA A    18
REMARK 465     ALA A    19
REMARK 465     LEU A    20
REMARK 465     SER A    21
REMARK 465     PRO A    22
REMARK 465     ALA A    23
REMARK 465     ALA A    24
REMARK 465     GLN A    25
REMARK 465     ALA A    26
REMARK 465     GLY A    27
REMARK 465     GLU A    28
REMARK 465     SER A    29
REMARK 465     THR A   254
REMARK 465     LEU A   255
REMARK 465     SER A   727
REMARK 465     ALA A   728
REMARK 465     TRP A   729
REMARK 465     SER A   730
REMARK 465     HIS A   731
REMARK 465     PRO A   732
REMARK 465     GLN A   733
REMARK 465     PHE A   734
REMARK 465     GLU A   735
REMARK 465     LYS A   736
REMARK 465     MSE B   -19
REMARK 465     GLY B   -18
REMARK 465     SER B   -17
REMARK 465     SER B   -16
REMARK 465     HIS B   -15
REMARK 465     HIS B   -14
REMARK 465     HIS B   -13
REMARK 465     HIS B   -12
REMARK 465     HIS B   -11
REMARK 465     HIS B   -10
REMARK 465     SER B    -9
REMARK 465     SER B    -8
REMARK 465     GLY B    -7
REMARK 465     LEU B    -6
REMARK 465     VAL B    -5
REMARK 465     PRO B    -4
REMARK 465     ARG B    -3
REMARK 465     GLY B    -2
REMARK 465     SER B    -1
REMARK 465     HIS B     0
REMARK 465     MSE B     1
REMARK 465     LYS B     2
REMARK 465     ILE B     3
REMARK 465     ALA B     4
REMARK 465     LEU B     5
REMARK 465     ARG B     6
REMARK 465     SER B     7
REMARK 465     GLY B     8
REMARK 465     LEU B     9
REMARK 465     PRO B    10
REMARK 465     ALA B    11
REMARK 465     VAL B    12
REMARK 465     ALA B    13
REMARK 465     LEU B    14
REMARK 465     LEU B    15
REMARK 465     ALA B    16
REMARK 465     ALA B    17
REMARK 465     ALA B    18
REMARK 465     ALA B    19
REMARK 465     LEU B    20
REMARK 465     SER B    21
REMARK 465     PRO B    22
REMARK 465     ALA B    23
REMARK 465     ALA B    24
REMARK 465     GLN B    25
REMARK 465     ALA B    26
REMARK 465     GLY B    27
REMARK 465     GLU B    28
REMARK 465     THR B   250
REMARK 465     SER B   251
REMARK 465     ASP B   252
REMARK 465     GLY B   253
REMARK 465     THR B   254
REMARK 465     LEU B   255
REMARK 465     GLY B   256
REMARK 465     GLY B   257
REMARK 465     ALA B   258
REMARK 465     PHE B   259
REMARK 465     GLU B   260
REMARK 465     GLN B   277
REMARK 465     ARG B   278
REMARK 465     ASP B   279
REMARK 465     LEU B   280
REMARK 465     LEU B   281
REMARK 465     SER B   282
REMARK 465     MSE B   283
REMARK 465     ALA B   724
REMARK 465     ALA B   725
REMARK 465     PRO B   726
REMARK 465     SER B   727
REMARK 465     ALA B   728
REMARK 465     TRP B   729
REMARK 465     SER B   730
REMARK 465     HIS B   731
REMARK 465     PRO B   732
REMARK 465     GLN B   733
REMARK 465     PHE B   734
REMARK 465     GLU B   735
REMARK 465     LYS B   736
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     SER B 284    CB   OG
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   NZ   LYS A   710     OD2  ASP B   188              1.81
REMARK 500   NH1  ARG A   446     OE2  GLU A   485              1.93
REMARK 500   NH1  ARG A    32     OD2  ASP A    38              2.08
REMARK 500   OE2  GLU B   243     NH1  ARG B   272              2.08
REMARK 500   NH1  ARG A   428     OE2  GLU A   545              2.09
REMARK 500   NH2  ARG B   433     OD2  ASP B   537              2.13
REMARK 500   O    ASP A   624     NH1  ARG A   721              2.14
REMARK 500   OG1  THR B   670     O    VAL B   672              2.15
REMARK 500   NH2  ARG B    32     OD2  ASP B    38              2.17
REMARK 500   O    LEU A   280     OG   SER A   284              2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PRO A  33     -175.82    -69.52
REMARK 500    LEU A  88      -66.61   -101.23
REMARK 500    LEU A 130      -62.63   -107.60
REMARK 500    LEU A 148      -73.03    -82.59
REMARK 500    ALA A 258      174.36    177.93
REMARK 500    VAL A 267      -69.83    -98.62
REMARK 500    THR A 373      -64.45    -97.16
REMARK 500    ASP A 466     -169.89   -160.37
REMARK 500    ASN A 474      -62.87   -106.77
REMARK 500    TRP A 476     -123.40     52.72
REMARK 500    TRP A 528      -63.24   -127.40
REMARK 500    SER A 559     -114.57     55.70
REMARK 500    LEU A 586       -6.17     79.81
REMARK 500    ALA A 628      175.46    173.97
REMARK 500    LEU B 130      -72.37   -108.37
REMARK 500    LYS B 169      -64.79    -99.05
REMARK 500    ASP B 188      -72.01    -43.69
REMARK 500    ILE B 220      -70.01    -99.18
REMARK 500    THR B 231      -71.64   -103.80
REMARK 500    THR B 373      -64.39    -92.76
REMARK 500    ILE B 413      -62.66   -105.67
REMARK 500    ALA B 451       -5.84     66.89
REMARK 500    ASN B 474      -63.04   -106.58
REMARK 500    TRP B 476     -123.21     52.46
REMARK 500    PRO B 479       76.07    -68.16
REMARK 500    TRP B 528      -63.60   -121.32
REMARK 500    SER B 559     -116.93     56.48
REMARK 500    LEU B 586       -5.31     79.07
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue BGC A 801
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue BGC B 801
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 5JQF   RELATED DB: PDB
REMARK 900 5JQF (SPHINGOPYXIN I) IS THE SUBSTRATE FOR SPI-ISOP
REMARK 900 RELATED ID: 5JRL   RELATED DB: PDB
REMARK 900 5JRL IS THE NATIVE FORM OF THIS PROTEIN
DBREF  5JRK A    1   726  UNP    Q1GQ33   Q1GQ33_SPHAL     1    726
DBREF  5JRK B    1   726  UNP    Q1GQ33   Q1GQ33_SPHAL     1    726
SEQADV 5JRK MSE A  -19  UNP  Q1GQ33              INITIATING METHIONINE
SEQADV 5JRK GLY A  -18  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER A  -17  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER A  -16  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS A  -15  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS A  -14  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS A  -13  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS A  -12  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS A  -11  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS A  -10  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER A   -9  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER A   -8  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK GLY A   -7  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK LEU A   -6  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK VAL A   -5  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK PRO A   -4  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK ARG A   -3  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK GLY A   -2  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER A   -1  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS A    0  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER A  727  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK ALA A  728  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK TRP A  729  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER A  730  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS A  731  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK PRO A  732  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK GLN A  733  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK PHE A  734  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK GLU A  735  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK LYS A  736  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK MSE B  -19  UNP  Q1GQ33              INITIATING METHIONINE
SEQADV 5JRK GLY B  -18  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER B  -17  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER B  -16  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS B  -15  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS B  -14  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS B  -13  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS B  -12  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS B  -11  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS B  -10  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER B   -9  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER B   -8  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK GLY B   -7  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK LEU B   -6  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK VAL B   -5  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK PRO B   -4  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK ARG B   -3  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK GLY B   -2  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER B   -1  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS B    0  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER B  727  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK ALA B  728  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK TRP B  729  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK SER B  730  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK HIS B  731  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK PRO B  732  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK GLN B  733  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK PHE B  734  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK GLU B  735  UNP  Q1GQ33              EXPRESSION TAG
SEQADV 5JRK LYS B  736  UNP  Q1GQ33              EXPRESSION TAG
SEQRES   1 A  756  MSE GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 A  756  LEU VAL PRO ARG GLY SER HIS MSE LYS ILE ALA LEU ARG
SEQRES   3 A  756  SER GLY LEU PRO ALA VAL ALA LEU LEU ALA ALA ALA ALA
SEQRES   4 A  756  LEU SER PRO ALA ALA GLN ALA GLY GLU SER LYS GLY ARG
SEQRES   5 A  756  PRO TRP THR LEU GLU ASP ILE LEU THR VAL PRO GLU VAL
SEQRES   6 A  756  ASN GLU ILE ALA LEU SER ASP ASN GLY ARG LEU ALA ILE
SEQRES   7 A  756  TYR ALA ALA GLU ILE ALA ASP LEU ASP ALA GLY LYS PRO
SEQRES   8 A  756  ARG SER HIS ILE ARG ILE VAL ASP VAL GLU THR GLY ARG
SEQRES   9 A  756  THR LYS GLU LEU LEU THR VAL ASP THR ILE LYS SER LEU
SEQRES  10 A  756  ARG SER VAL PRO GLY THR GLN ASP TRP SER ALA LEU VAL
SEQRES  11 A  756  ASP LEU GLY GLU GLY GLN GLN LEU TYR ARG ILE ASP THR
SEQRES  12 A  756  GLU GLY LYS LEU LEU PRO LEU ILE VAL ASN PRO ASN PRO
SEQRES  13 A  756  VAL PRO VAL GLY LYS ALA ASP MSE SER PHE PRO LEU GLY
SEQRES  14 A  756  GLY GLY ILE ARG PRO SER HIS ILE GLY ILE LEU ASP TYR
SEQRES  15 A  756  ASP TRP SER PRO ASP GLY LYS TRP LEU TRP TYR SER GLN
SEQRES  16 A  756  LEU ARG ALA LYS SER ASP GLY PRO ARG VAL ARG PHE ASP
SEQRES  17 A  756  GLU GLU VAL THR ALA LEU LEU GLY ARG ARG ARG SER THR
SEQRES  18 A  756  ILE ASP VAL GLU VAL ASP PHE PHE LEU ARG ASN PRO GLU
SEQRES  19 A  756  GLY ASP THR THR ARG ILE MSE ALA ARG PRO SER THR ASP
SEQRES  20 A  756  ARG VAL ALA THR ARG GLY GLY GLY ARG VAL LEU TRP ARG
SEQRES  21 A  756  GLY ASN GLU VAL GLN PHE ARG ILE GLU THR SER ASP GLY
SEQRES  22 A  756  THR LEU GLY GLY ALA PHE GLU PHE VAL ALA TRP ASN ARG
SEQRES  23 A  756  VAL ASN ARG THR VAL ARG THR LEU ALA LYS GLN ARG ASP
SEQRES  24 A  756  LEU LEU SER MSE SER ILE LEU VAL GLY PRO ARG GLY GLY
SEQRES  25 A  756  GLN LEU SER THR SER GLY LEU GLY SER ASP ARG GLU LEU
SEQRES  26 A  756  ILE GLU THR SER ALA GLU GLY ARG PRO HIS SER TYR GLY
SEQRES  27 A  756  ARG VAL ALA PHE ASP ILE GLY ASP SER ARG SER ALA GLY
SEQRES  28 A  756  TRP LYS ARG SER ARG ASP GLY LYS ARG VAL VAL ILE GLY
SEQRES  29 A  756  THR ARG GLY LEU GLY ASP ALA ARG TYR GLY LEU ALA LEU
SEQRES  30 A  756  ILE ASP LYS THR GLY VAL ARG GLU LEU ARG ALA ASP ALA
SEQRES  31 A  756  SER LEU THR ARG CYS GLY PHE ASP GLY MSE LEU ARG SER
SEQRES  32 A  756  ALA ILE CYS VAL GLU GLU GLY MSE SER ARG PRO PRO ARG
SEQRES  33 A  756  LEU VAL ARG VAL ASP LEU GLY THR ASP LYS ILE THR ASP
SEQRES  34 A  756  LEU GLY PRO ILE SER PRO ARG HIS GLU GLU ILE GLU PRO
SEQRES  35 A  756  LEU GLN THR ILE ALA ARG THR PHE VAL SER ARG ASP GLY
SEQRES  36 A  756  TYR TRP SER SER GLY TYR VAL LEU LEU PRO ARG GLY HIS
SEQRES  37 A  756  ARG ALA ALA ASP ARG HIS PRO ALA VAL VAL VAL THR HIS
SEQRES  38 A  756  GLY THR ASP ALA ASP ASP ARG PHE ALA GLU PRO ALA ASN
SEQRES  39 A  756  GLN TRP ASN TYR PRO VAL GLN LEU LEU ALA GLU ARG GLY
SEQRES  40 A  756  TYR VAL VAL LEU LEU LEU ASN ASP PRO SER PRO GLY GLN
SEQRES  41 A  756  SER LYS ASP LEU MSE ASP ALA MSE HIS ALA TRP LEU ARG
SEQRES  42 A  756  GLY LYS GLY PRO PRO ASP PRO GLU THR VAL GLN GLN LYS
SEQRES  43 A  756  LEU TRP LEU THR GLY VAL HIS SER PHE GLU ASP ALA VAL
SEQRES  44 A  756  THR GLU LEU ALA ALA GLU GLY LEU ILE ASP PRO ALA ARG
SEQRES  45 A  756  VAL GLY ILE ALA GLY TYR SER ARG GLY SER GLN MSE VAL
SEQRES  46 A  756  ASN VAL THR VAL THR ASN SER LYS MSE PHE ARG ALA ALA
SEQRES  47 A  756  SER SER GLY ASP GLY GLY PHE LEU GLU PRO ALA GLY TYR
SEQRES  48 A  756  ALA THR GLY ARG SER SER TYR ASP ALA VAL TYR GLY GLY
SEQRES  49 A  756  ALA PRO LEU SER ASP ASN ILE GLU ARG TRP ARG ARG PHE
SEQRES  50 A  756  ALA PRO SER LEU ASN ALA ASP LYS VAL CYS ALA ALA VAL
SEQRES  51 A  756  LEU GLN GLN VAL ALA SER ALA SER PRO SER GLN ILE GLU
SEQRES  52 A  756  LEU PHE GLU ALA LEU ARG ALA ALA GLY VAL ALA THR GLN
SEQRES  53 A  756  ILE SER TYR TYR PRO GLY ALA THR ALA ALA SER ASP GLU
SEQRES  54 A  756  THR HIS VAL PHE TYR LEU THR THR ASN ARG LEU ARG ALA
SEQRES  55 A  756  MSE ARG GLU ASN ILE ALA TRP PHE ASP TYR TRP LEU LEU
SEQRES  56 A  756  ASP LYS ARG ASP ALA ASP ALA PRO PHE PRO ASP HIS VAL
SEQRES  57 A  756  VAL LYS TRP ASP ARG LEU LYS LYS ASN LEU PRO ASP ARG
SEQRES  58 A  756  CYS ALA ALA ALA PRO SER ALA TRP SER HIS PRO GLN PHE
SEQRES  59 A  756  GLU LYS
SEQRES   1 B  756  MSE GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 B  756  LEU VAL PRO ARG GLY SER HIS MSE LYS ILE ALA LEU ARG
SEQRES   3 B  756  SER GLY LEU PRO ALA VAL ALA LEU LEU ALA ALA ALA ALA
SEQRES   4 B  756  LEU SER PRO ALA ALA GLN ALA GLY GLU SER LYS GLY ARG
SEQRES   5 B  756  PRO TRP THR LEU GLU ASP ILE LEU THR VAL PRO GLU VAL
SEQRES   6 B  756  ASN GLU ILE ALA LEU SER ASP ASN GLY ARG LEU ALA ILE
SEQRES   7 B  756  TYR ALA ALA GLU ILE ALA ASP LEU ASP ALA GLY LYS PRO
SEQRES   8 B  756  ARG SER HIS ILE ARG ILE VAL ASP VAL GLU THR GLY ARG
SEQRES   9 B  756  THR LYS GLU LEU LEU THR VAL ASP THR ILE LYS SER LEU
SEQRES  10 B  756  ARG SER VAL PRO GLY THR GLN ASP TRP SER ALA LEU VAL
SEQRES  11 B  756  ASP LEU GLY GLU GLY GLN GLN LEU TYR ARG ILE ASP THR
SEQRES  12 B  756  GLU GLY LYS LEU LEU PRO LEU ILE VAL ASN PRO ASN PRO
SEQRES  13 B  756  VAL PRO VAL GLY LYS ALA ASP MSE SER PHE PRO LEU GLY
SEQRES  14 B  756  GLY GLY ILE ARG PRO SER HIS ILE GLY ILE LEU ASP TYR
SEQRES  15 B  756  ASP TRP SER PRO ASP GLY LYS TRP LEU TRP TYR SER GLN
SEQRES  16 B  756  LEU ARG ALA LYS SER ASP GLY PRO ARG VAL ARG PHE ASP
SEQRES  17 B  756  GLU GLU VAL THR ALA LEU LEU GLY ARG ARG ARG SER THR
SEQRES  18 B  756  ILE ASP VAL GLU VAL ASP PHE PHE LEU ARG ASN PRO GLU
SEQRES  19 B  756  GLY ASP THR THR ARG ILE MSE ALA ARG PRO SER THR ASP
SEQRES  20 B  756  ARG VAL ALA THR ARG GLY GLY GLY ARG VAL LEU TRP ARG
SEQRES  21 B  756  GLY ASN GLU VAL GLN PHE ARG ILE GLU THR SER ASP GLY
SEQRES  22 B  756  THR LEU GLY GLY ALA PHE GLU PHE VAL ALA TRP ASN ARG
SEQRES  23 B  756  VAL ASN ARG THR VAL ARG THR LEU ALA LYS GLN ARG ASP
SEQRES  24 B  756  LEU LEU SER MSE SER ILE LEU VAL GLY PRO ARG GLY GLY
SEQRES  25 B  756  GLN LEU SER THR SER GLY LEU GLY SER ASP ARG GLU LEU
SEQRES  26 B  756  ILE GLU THR SER ALA GLU GLY ARG PRO HIS SER TYR GLY
SEQRES  27 B  756  ARG VAL ALA PHE ASP ILE GLY ASP SER ARG SER ALA GLY
SEQRES  28 B  756  TRP LYS ARG SER ARG ASP GLY LYS ARG VAL VAL ILE GLY
SEQRES  29 B  756  THR ARG GLY LEU GLY ASP ALA ARG TYR GLY LEU ALA LEU
SEQRES  30 B  756  ILE ASP LYS THR GLY VAL ARG GLU LEU ARG ALA ASP ALA
SEQRES  31 B  756  SER LEU THR ARG CYS GLY PHE ASP GLY MSE LEU ARG SER
SEQRES  32 B  756  ALA ILE CYS VAL GLU GLU GLY MSE SER ARG PRO PRO ARG
SEQRES  33 B  756  LEU VAL ARG VAL ASP LEU GLY THR ASP LYS ILE THR ASP
SEQRES  34 B  756  LEU GLY PRO ILE SER PRO ARG HIS GLU GLU ILE GLU PRO
SEQRES  35 B  756  LEU GLN THR ILE ALA ARG THR PHE VAL SER ARG ASP GLY
SEQRES  36 B  756  TYR TRP SER SER GLY TYR VAL LEU LEU PRO ARG GLY HIS
SEQRES  37 B  756  ARG ALA ALA ASP ARG HIS PRO ALA VAL VAL VAL THR HIS
SEQRES  38 B  756  GLY THR ASP ALA ASP ASP ARG PHE ALA GLU PRO ALA ASN
SEQRES  39 B  756  GLN TRP ASN TYR PRO VAL GLN LEU LEU ALA GLU ARG GLY
SEQRES  40 B  756  TYR VAL VAL LEU LEU LEU ASN ASP PRO SER PRO GLY GLN
SEQRES  41 B  756  SER LYS ASP LEU MSE ASP ALA MSE HIS ALA TRP LEU ARG
SEQRES  42 B  756  GLY LYS GLY PRO PRO ASP PRO GLU THR VAL GLN GLN LYS
SEQRES  43 B  756  LEU TRP LEU THR GLY VAL HIS SER PHE GLU ASP ALA VAL
SEQRES  44 B  756  THR GLU LEU ALA ALA GLU GLY LEU ILE ASP PRO ALA ARG
SEQRES  45 B  756  VAL GLY ILE ALA GLY TYR SER ARG GLY SER GLN MSE VAL
SEQRES  46 B  756  ASN VAL THR VAL THR ASN SER LYS MSE PHE ARG ALA ALA
SEQRES  47 B  756  SER SER GLY ASP GLY GLY PHE LEU GLU PRO ALA GLY TYR
SEQRES  48 B  756  ALA THR GLY ARG SER SER TYR ASP ALA VAL TYR GLY GLY
SEQRES  49 B  756  ALA PRO LEU SER ASP ASN ILE GLU ARG TRP ARG ARG PHE
SEQRES  50 B  756  ALA PRO SER LEU ASN ALA ASP LYS VAL CYS ALA ALA VAL
SEQRES  51 B  756  LEU GLN GLN VAL ALA SER ALA SER PRO SER GLN ILE GLU
SEQRES  52 B  756  LEU PHE GLU ALA LEU ARG ALA ALA GLY VAL ALA THR GLN
SEQRES  53 B  756  ILE SER TYR TYR PRO GLY ALA THR ALA ALA SER ASP GLU
SEQRES  54 B  756  THR HIS VAL PHE TYR LEU THR THR ASN ARG LEU ARG ALA
SEQRES  55 B  756  MSE ARG GLU ASN ILE ALA TRP PHE ASP TYR TRP LEU LEU
SEQRES  56 B  756  ASP LYS ARG ASP ALA ASP ALA PRO PHE PRO ASP HIS VAL
SEQRES  57 B  756  VAL LYS TRP ASP ARG LEU LYS LYS ASN LEU PRO ASP ARG
SEQRES  58 B  756  CYS ALA ALA ALA PRO SER ALA TRP SER HIS PRO GLN PHE
SEQRES  59 B  756  GLU LYS
MODRES 5JRK MSE A  144  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  221  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  283  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  380  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  391  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  505  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  508  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  564  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  574  MET  MODIFIED RESIDUE
MODRES 5JRK MSE A  683  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  144  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  221  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  380  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  391  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  505  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  508  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  564  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  574  MET  MODIFIED RESIDUE
MODRES 5JRK MSE B  683  MET  MODIFIED RESIDUE
HET    MSE  A 144       8
HET    MSE  A 221       8
HET    MSE  A 283       8
HET    MSE  A 380       8
HET    MSE  A 391       8
HET    MSE  A 505       8
HET    MSE  A 508       8
HET    MSE  A 564       8
HET    MSE  A 574       8
HET    MSE  A 683       8
HET    MSE  B 144       8
HET    MSE  B 221       8
HET    MSE  B 380       8
HET    MSE  B 391       8
HET    MSE  B 505       8
HET    MSE  B 508       8
HET    MSE  B 564       8
HET    MSE  B 574       8
HET    MSE  B 683       8
HET    BGC  A 801      12
HET    BGC  B 801      12
HETNAM     MSE SELENOMETHIONINE
HETNAM     BGC BETA-D-GLUCOSE
FORMUL   1  MSE    19(C5 H11 N O2 SE)
FORMUL   3  BGC    2(C6 H12 O6)
HELIX    1 AA1 THR A   35  THR A   41  1                                   7
HELIX    2 AA2 PHE A  187  ALA A  193  1                                   7
HELIX    3 AA3 ASP A  227  GLY A  233  1                                   7
HELIX    4 AA4 SER A  414  GLU A  419  1                                   6
HELIX    5 AA5 GLU A  471  TRP A  476  1                                   6
HELIX    6 AA6 PRO A  479  ARG A  486  1                                   8
HELIX    7 AA7 SER A  497  GLN A  500  5                                   4
HELIX    8 AA8 SER A  501  GLY A  514  1                                  14
HELIX    9 AA9 ASP A  519  TRP A  528  1                                  10
HELIX   10 AB1 TRP A  528  GLU A  545  1                                  18
HELIX   11 AB2 SER A  559  SER A  572  1                                  14
HELIX   12 AB3 GLU A  587  ALA A  589  5                                   3
HELIX   13 AB4 GLY A  590  GLY A  603  1                                  14
HELIX   14 AB5 ASN A  610  ALA A  618  1                                   9
HELIX   15 AB6 ASN A  622  VAL A  626  5                                   5
HELIX   16 AB7 SER A  638  ALA A  651  1                                  14
HELIX   17 AB8 LEU A  675  LEU A  694  1                                  20
HELIX   18 AB9 ASP A  706  ASN A  717  1                                  12
HELIX   19 AC1 PRO A  719  ALA A  723  5                                   5
HELIX   20 AC2 THR B   35  THR B   41  1                                   7
HELIX   21 AC3 PHE B  187  THR B  192  1                                   6
HELIX   22 AC4 ALA B  193  LEU B  195  5                                   3
HELIX   23 AC5 SER B  414  ILE B  420  1                                   7
HELIX   24 AC6 GLU B  471  TRP B  476  1                                   6
HELIX   25 AC7 PRO B  479  ARG B  486  1                                   8
HELIX   26 AC8 SER B  497  GLN B  500  5                                   4
HELIX   27 AC9 SER B  501  GLY B  514  1                                  14
HELIX   28 AD1 ASP B  519  TRP B  528  1                                  10
HELIX   29 AD2 TRP B  528  GLU B  545  1                                  18
HELIX   30 AD3 SER B  559  SER B  572  1                                  14
HELIX   31 AD4 GLU B  587  ALA B  589  5                                   3
HELIX   32 AD5 GLY B  590  GLY B  603  1                                  14
HELIX   33 AD6 ASN B  610  ALA B  618  1                                   9
HELIX   34 AD7 ASN B  622  VAL B  626  5                                   5
HELIX   35 AD8 SER B  638  ALA B  651  1                                  14
HELIX   36 AD9 LEU B  675  LEU B  694  1                                  20
HELIX   37 AE1 PRO B  705  ASN B  717  1                                  13
SHEET    1 AA1 4 GLU A  44  LEU A  50  0
SHEET    2 AA1 4 LEU A  56  ALA A  64 -1  O  GLU A  62   N  GLU A  44
SHEET    3 AA1 4 PRO A  71  ASP A  79 -1  O  ARG A  72   N  ILE A  63
SHEET    4 AA1 4 THR A  85  VAL A  91 -1  O  LYS A  86   N  ILE A  77
SHEET    1 AA2 4 THR A  93  SER A  99  0
SHEET    2 AA2 4 TRP A 106  ASP A 111 -1  O  LEU A 109   N  LYS A  95
SHEET    3 AA2 4 GLN A 116  ILE A 121 -1  O  ILE A 121   N  TRP A 106
SHEET    4 AA2 4 LEU A 127  ILE A 131 -1  O  LEU A 128   N  ARG A 120
SHEET    1 AA3 2 VAL A 137  VAL A 139  0
SHEET    2 AA3 2 SER A 155  ILE A 157 -1  O  SER A 155   N  VAL A 139
SHEET    1 AA4 4 ASP A 161  TRP A 164  0
SHEET    2 AA4 4 LEU A 171  ALA A 178 -1  O  TRP A 172   N  ASP A 163
SHEET    3 AA4 4 VAL A 204  ARG A 211 -1  O  PHE A 209   N  TYR A 173
SHEET    4 AA4 4 THR A 217  ARG A 223 -1  O  ILE A 220   N  PHE A 208
SHEET    1 AA5 4 VAL A 237  ARG A 240  0
SHEET    2 AA5 4 GLU A 243  ILE A 248 -1  O  GLN A 245   N  LEU A 238
SHEET    3 AA5 4 PHE A 259  ASN A 265 -1  O  TRP A 264   N  VAL A 244
SHEET    4 AA5 4 VAL A 271  LYS A 276 -1  O  LEU A 274   N  PHE A 261
SHEET    1 AA6 3 GLN A 293  SER A 297  0
SHEET    2 AA6 3 ARG A 303  THR A 308 -1  O  THR A 308   N  GLN A 293
SHEET    3 AA6 3 PRO A 314  VAL A 320 -1  O  HIS A 315   N  GLU A 307
SHEET    1 AA7 3 PHE A 322  ILE A 324  0
SHEET    2 AA7 3 VAL A 341  GLY A 347 -1  O  ARG A 346   N  ASP A 323
SHEET    3 AA7 3 TRP A 332  ARG A 334 -1  N  LYS A 333   O  VAL A 342
SHEET    1 AA8 4 PHE A 322  ILE A 324  0
SHEET    2 AA8 4 VAL A 341  GLY A 347 -1  O  ARG A 346   N  ASP A 323
SHEET    3 AA8 4 TYR A 353  ILE A 358 -1  O  GLY A 354   N  THR A 345
SHEET    4 AA8 4 VAL A 363  GLU A 365 -1  O  ARG A 364   N  LEU A 357
SHEET    1 AA9 4 SER A 371  PHE A 377  0
SHEET    2 AA9 4 SER A 383  GLU A 389 -1  O  GLU A 389   N  SER A 371
SHEET    3 AA9 4 ARG A 396  ASP A 401 -1  O  VAL A 400   N  ALA A 384
SHEET    4 AA9 4 LYS A 406  PRO A 412 -1  O  LYS A 406   N  ASP A 401
SHEET    1 AB1 8 GLN A 424  ALA A 427  0
SHEET    2 AB1 8 GLY A 440  LEU A 444 -1  O  VAL A 442   N  ILE A 426
SHEET    3 AB1 8 VAL A 489  LEU A 493 -1  O  VAL A 490   N  LEU A 443
SHEET    4 AB1 8 HIS A 454  VAL A 459  1  N  VAL A 457   O  LEU A 491
SHEET    5 AB1 8 ILE A 548  TYR A 558  1  O  GLY A 554   N  VAL A 458
SHEET    6 AB1 8 ALA A 577  GLY A 581  1  O  GLY A 581   N  GLY A 557
SHEET    7 AB1 8 ALA A 629  VAL A 634  1  O  ALA A 629   N  ALA A 578
SHEET    8 AB1 8 ALA A 654  TYR A 659  1  O  SER A 658   N  VAL A 634
SHEET    1 AB2 2 PHE A 430  VAL A 431  0
SHEET    2 AB2 2 TRP A 437  SER A 438 -1  O  SER A 438   N  PHE A 430
SHEET    1 AB3 4 GLU B  44  LEU B  50  0
SHEET    2 AB3 4 LEU B  56  ALA B  64 -1  O  ILE B  58   N  ALA B  49
SHEET    3 AB3 4 PRO B  71  ASP B  79 -1  O  VAL B  78   N  ALA B  57
SHEET    4 AB3 4 THR B  85  VAL B  91 -1  O  VAL B  91   N  SER B  73
SHEET    1 AB4 4 THR B  93  SER B  99  0
SHEET    2 AB4 4 TRP B 106  ASP B 111 -1  O  LEU B 109   N  LYS B  95
SHEET    3 AB4 4 GLN B 116  ILE B 121 -1  O  ILE B 121   N  TRP B 106
SHEET    4 AB4 4 LEU B 127  ILE B 131 -1  O  LEU B 128   N  ARG B 120
SHEET    1 AB5 2 VAL B 137  VAL B 139  0
SHEET    2 AB5 2 SER B 155  ILE B 157 -1  O  SER B 155   N  VAL B 139
SHEET    1 AB6 4 ILE B 159  TRP B 164  0
SHEET    2 AB6 4 LEU B 171  ALA B 178 -1  O  SER B 174   N  ASP B 161
SHEET    3 AB6 4 VAL B 204  ARG B 211 -1  O  ARG B 211   N  LEU B 171
SHEET    4 AB6 4 THR B 217  ARG B 223 -1  O  ILE B 220   N  PHE B 208
SHEET    1 AB7 4 VAL B 237  ARG B 240  0
SHEET    2 AB7 4 GLU B 243  PHE B 246 -1  O  GLN B 245   N  LEU B 238
SHEET    3 AB7 4 VAL B 262  ASN B 265 -1  O  TRP B 264   N  VAL B 244
SHEET    4 AB7 4 THR B 270  ARG B 272 -1  O  ARG B 272   N  ALA B 263
SHEET    1 AB8 3 GLN B 293  SER B 297  0
SHEET    2 AB8 3 ARG B 303  THR B 308 -1  O  ILE B 306   N  SER B 295
SHEET    3 AB8 3 PRO B 314  VAL B 320 -1  O  VAL B 320   N  ARG B 303
SHEET    1 AB9 3 PHE B 322  ASP B 323  0
SHEET    2 AB9 3 ARG B 340  GLY B 347 -1  O  ARG B 346   N  ASP B 323
SHEET    3 AB9 3 TRP B 332  ARG B 334 -1  N  LYS B 333   O  VAL B 342
SHEET    1 AC1 4 PHE B 322  ASP B 323  0
SHEET    2 AC1 4 ARG B 340  GLY B 347 -1  O  ARG B 346   N  ASP B 323
SHEET    3 AC1 4 TYR B 353  ASP B 359 -1  O  ILE B 358   N  VAL B 341
SHEET    4 AC1 4 VAL B 363  GLU B 365 -1  O  ARG B 364   N  LEU B 357
SHEET    1 AC2 4 SER B 371  PHE B 377  0
SHEET    2 AC2 4 SER B 383  GLU B 389 -1  O  ILE B 385   N  GLY B 376
SHEET    3 AC2 4 ARG B 396  ASP B 401 -1  O  VAL B 400   N  ALA B 384
SHEET    4 AC2 4 LYS B 406  PRO B 412 -1  O  LEU B 410   N  LEU B 397
SHEET    1 AC3 8 THR B 425  VAL B 431  0
SHEET    2 AC3 8 TRP B 437  LEU B 443 -1  O  VAL B 442   N  ILE B 426
SHEET    3 AC3 8 VAL B 489  LEU B 493 -1  O  VAL B 490   N  LEU B 443
SHEET    4 AC3 8 HIS B 454  VAL B 459  1  N  VAL B 457   O  LEU B 491
SHEET    5 AC3 8 ILE B 548  TYR B 558  1  O  ALA B 556   N  VAL B 458
SHEET    6 AC3 8 ALA B 577  GLY B 581  1  O  GLY B 581   N  GLY B 557
SHEET    7 AC3 8 ALA B 629  VAL B 634  1  O  ALA B 629   N  ALA B 578
SHEET    8 AC3 8 THR B 655  TYR B 659  1  O  SER B 658   N  GLN B 632
SSBOND   1 CYS A  375    CYS A  386                          1555   1555  2.03
SSBOND   2 CYS A  627    CYS A  722                          1555   1555  2.03
SSBOND   3 CYS B  375    CYS B  386                          1555   1555  2.03
SSBOND   4 CYS B  627    CYS B  722                          1555   1555  2.03
LINK         C   ASP A 143                 N   MSE A 144     1555   1555  1.33
LINK         C   MSE A 144                 N   SER A 145     1555   1555  1.33
LINK         C   ILE A 220                 N   MSE A 221     1555   1555  1.33
LINK         C   MSE A 221                 N   ALA A 222     1555   1555  1.33
LINK         C   SER A 282                 N   MSE A 283     1555   1555  1.33
LINK         C   MSE A 283                 N   SER A 284     1555   1555  1.33
LINK         C   GLY A 379                 N   MSE A 380     1555   1555  1.33
LINK         C   MSE A 380                 N   LEU A 381     1555   1555  1.33
LINK         C   GLY A 390                 N   MSE A 391     1555   1555  1.33
LINK         C   MSE A 391                 N   SER A 392     1555   1555  1.33
LINK         C   LEU A 504                 N   MSE A 505     1555   1555  1.33
LINK         C   MSE A 505                 N   ASP A 506     1555   1555  1.34
LINK         C   ALA A 507                 N   MSE A 508     1555   1555  1.33
LINK         C   MSE A 508                 N   HIS A 509     1555   1555  1.33
LINK         C   GLN A 563                 N   MSE A 564     1555   1555  1.33
LINK         C   MSE A 564                 N   VAL A 565     1555   1555  1.33
LINK         C   LYS A 573                 N   MSE A 574     1555   1555  1.33
LINK         C   MSE A 574                 N   PHE A 575     1555   1555  1.33
LINK         C   ALA A 682                 N   MSE A 683     1555   1555  1.33
LINK         C   MSE A 683                 N   ARG A 684     1555   1555  1.33
LINK         C   ASP B 143                 N   MSE B 144     1555   1555  1.33
LINK         C   MSE B 144                 N   SER B 145     1555   1555  1.33
LINK         C   ILE B 220                 N   MSE B 221     1555   1555  1.33
LINK         C   MSE B 221                 N   ALA B 222     1555   1555  1.33
LINK         C   GLY B 379                 N   MSE B 380     1555   1555  1.33
LINK         C   MSE B 380                 N   LEU B 381     1555   1555  1.33
LINK         C   GLY B 390                 N   MSE B 391     1555   1555  1.33
LINK         C   MSE B 391                 N   SER B 392     1555   1555  1.33
LINK         C   LEU B 504                 N   MSE B 505     1555   1555  1.33
LINK         C   MSE B 505                 N   ASP B 506     1555   1555  1.33
LINK         C   ALA B 507                 N   MSE B 508     1555   1555  1.33
LINK         C   MSE B 508                 N   HIS B 509     1555   1555  1.33
LINK         C   GLN B 563                 N   MSE B 564     1555   1555  1.33
LINK         C   MSE B 564                 N   VAL B 565     1555   1555  1.33
LINK         C   LYS B 573                 N   MSE B 574     1555   1555  1.33
LINK         C   MSE B 574                 N   PHE B 575     1555   1555  1.33
LINK         C   ALA B 682                 N   MSE B 683     1555   1555  1.33
LINK         C   MSE B 683                 N   ARG B 684     1555   1555  1.34
CISPEP   1 GLY A  516    PRO A  517          0        -3.33
CISPEP   2 PRO A  517    PRO A  518          0        -0.51
CISPEP   3 GLY B  516    PRO B  517          0         0.26
CISPEP   4 PRO B  517    PRO B  518          0         1.30
SITE     1 AC1  6 ALA A  49  LEU A  50  SER A  99  LYS A 333
SITE     2 AC1  6 GLY A 376  PHE A 377
SITE     1 AC2  4 ASP B 369  GLU B 388  ILE B 407  ASP B 409
CRYST1  111.105  133.501  169.070  90.00  90.00  90.00 P 21 21 21    8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.009000  0.000000  0.000000        0.00000
SCALE2      0.000000  0.007491  0.000000        0.00000
SCALE3      0.000000  0.000000  0.005915        0.00000
TER    5394      PRO A 726
TER   10659      ALA B 723
MASTER      443    0   21   37   82    0    3    610681    2  222  118
END