longtext: 5lka-pdb

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HEADER    HYDROLASE                               21-JUL-16   5LKA
TITLE     CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE LINB 140A+143L+177W+211L
TITLE    2 MUTANT (LINB86) FROM SPHINGOBIUM JAPONICUM UT26 AT 1.3 A RESOLUTION
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: HALOALKANE DEHALOGENASE;
COMPND   3 CHAIN: A;
COMPND   4 EC: 3.8.1.5;
COMPND   5 ENGINEERED: YES;
COMPND   6 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: SPHINGOBIUM JAPONICUM;
SOURCE   3 ORGANISM_TAXID: 332056;
SOURCE   4 GENE: LINB, DHAA, SJA_C1-19590;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    HALOALKANE DEHALOGENASE, BACTERIAL ENZYME, MUTANT, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    O.DEGTJARIK,P.REZACOVA,I.IERMAK,R.CHALOUPKOVA,J.DAMBORSKY,I.KUTA
AUTHOR   2 SMATANOVA
REVDAT   1   05-OCT-16 5LKA    0
JRNL        AUTH   J.BREZOVSKY,P.BABKOVA,O.DEGTJARIK,A.FORTOVA,A.GORA,I.IERMAK,
JRNL        AUTH 2 P.REZACOVA,P.DVORAK,I.KUTA SMATANOVA,Z.PROKOP,R.CHALOUPKOVA,
JRNL        AUTH 3 J.DAMBORSKY
JRNL        TITL   ENGINEERING A DE NOVO TRANSPORT TUNNEL.
JRNL        REF    ACS CATALYSIS                              2016
JRNL        REFN                   ESSN 2155-5435
JRNL        DOI    10.1021/ACSCATAL.6B02081
REMARK   2
REMARK   2 RESOLUTION.    1.30 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.30
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 40.52
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.6
REMARK   3   NUMBER OF REFLECTIONS             : 64467
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.140
REMARK   3   R VALUE            (WORKING SET) : 0.139
REMARK   3   FREE R VALUE                     : 0.175
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 3.260
REMARK   3   FREE R VALUE TEST SET COUNT      : 2101
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 40.5413 -  3.2009    1.00     4423   149  0.1307 0.1548
REMARK   3     2  3.2009 -  2.5407    1.00     4248   143  0.1471 0.1503
REMARK   3     3  2.5407 -  2.2196    1.00     4207   142  0.1396 0.1654
REMARK   3     4  2.2196 -  2.0167    1.00     4173   141  0.1313 0.1926
REMARK   3     5  2.0167 -  1.8721    1.00     4173   140  0.1272 0.1811
REMARK   3     6  1.8721 -  1.7617    1.00     4142   140  0.1235 0.1553
REMARK   3     7  1.7617 -  1.6735    1.00     4143   139  0.1224 0.1592
REMARK   3     8  1.6735 -  1.6007    1.00     4173   140  0.1200 0.1791
REMARK   3     9  1.6007 -  1.5390    1.00     4128   139  0.1272 0.1746
REMARK   3    10  1.5390 -  1.4859    1.00     4111   139  0.1298 0.1668
REMARK   3    11  1.4859 -  1.4395    1.00     4146   140  0.1417 0.1865
REMARK   3    12  1.4395 -  1.3983    1.00     4104   138  0.1655 0.2405
REMARK   3    13  1.3983 -  1.3615    1.00     4105   138  0.1791 0.2262
REMARK   3    14  1.3615 -  1.3283    1.00     4115   139  0.1891 0.2614
REMARK   3    15  1.3283 -  1.2981    0.97     3975   134  0.2047 0.2266
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : NULL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.120
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 16.130
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 14.49
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 12.69
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.006           2623
REMARK   3   ANGLE     :  0.921           3595
REMARK   3   CHIRALITY :  0.085            370
REMARK   3   PLANARITY :  0.006            487
REMARK   3   DIHEDRAL  : 21.922            992
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 5LKA COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 22-JUL-16.
REMARK 100 THE DEPOSITION ID IS D_1200000874.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 31-JAN-15
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : BESSY
REMARK 200  BEAMLINE                       : 14.1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9184
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS NOVEMBER 3, 2014
REMARK 200  DATA SCALING SOFTWARE          : XDS NOVEMBER 3, 2014
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 64480
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.298
REMARK 200  RESOLUTION RANGE LOW       (A) : 46.870
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.6
REMARK 200  DATA REDUNDANCY                : 6.350
REMARK 200  R MERGE                    (I) : 0.08300
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 16.4500
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.30
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.38
REMARK 200  COMPLETENESS FOR SHELL     (%) : 98.7
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 0.50600
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 3.500
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER 2.6.0
REMARK 200 STARTING MODEL: 4WDR
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 35.71
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 1.91
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M SODIUM THIOCYANATE, 20 % (W/V)
REMARK 280  PEG 3350., VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       23.43350
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       40.32400
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       34.18100
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       40.32400
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       23.43350
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       34.18100
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 230 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 11720 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -5.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     LYS A   6    CD   CE   NZ
REMARK 470     HIS A 302    CG   ND1  CD2  CE1  NE2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PRO A  39       51.36   -104.62
REMARK 500    THR A  40     -162.36   -103.23
REMARK 500    HIS A 107     -114.01   -108.43
REMARK 500    ASP A 108     -130.63     63.21
REMARK 500    ASP A 108      -91.45    -90.80
REMARK 500    HIS A 121       47.88   -140.07
REMARK 500    ALA A 247      -64.11   -152.66
REMARK 500    THR A 249       74.02   -116.93
REMARK 500    ALA A 271      -96.50    -98.27
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SCN A 401
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4WDR   RELATED DB: PDB
REMARK 900 SAME VARIANT, H32 SPACE GROUP, 2.5A RESOLUTION
REMARK 900 RELATED ID: 4WDQ   RELATED DB: PDB
REMARK 900 LINB L177W VARIANT
REMARK 900 RELATED ID: 1CV2   RELATED DB: PDB
REMARK 900 LINB WILD TYPE
DBREF  5LKA A    2   296  UNP    D4Z2G1   D4Z2G1_SPHJU     2    296
SEQADV 5LKA ALA A  140  UNP  D4Z2G1    TRP   140 ENGINEERED MUTATION
SEQADV 5LKA LEU A  143  UNP  D4Z2G1    PHE   143 ENGINEERED MUTATION
SEQADV 5LKA TRP A  177  UNP  D4Z2G1    LEU   177 ENGINEERED MUTATION
SEQADV 5LKA LEU A  211  UNP  D4Z2G1    ILE   211 ENGINEERED MUTATION
SEQADV 5LKA HIS A  297  UNP  D4Z2G1              EXPRESSION TAG
SEQADV 5LKA HIS A  298  UNP  D4Z2G1              EXPRESSION TAG
SEQADV 5LKA HIS A  299  UNP  D4Z2G1              EXPRESSION TAG
SEQADV 5LKA HIS A  300  UNP  D4Z2G1              EXPRESSION TAG
SEQADV 5LKA HIS A  301  UNP  D4Z2G1              EXPRESSION TAG
SEQADV 5LKA HIS A  302  UNP  D4Z2G1              EXPRESSION TAG
SEQRES   1 A  301  SER LEU GLY ALA LYS PRO PHE GLY GLU LYS LYS PHE ILE
SEQRES   2 A  301  GLU ILE LYS GLY ARG ARG MET ALA TYR ILE ASP GLU GLY
SEQRES   3 A  301  THR GLY ASP PRO ILE LEU PHE GLN HIS GLY ASN PRO THR
SEQRES   4 A  301  SER SER TYR LEU TRP ARG ASN ILE MET PRO HIS CYS ALA
SEQRES   5 A  301  GLY LEU GLY ARG LEU ILE ALA CYS ASP LEU ILE GLY MET
SEQRES   6 A  301  GLY ASP SER ASP LYS LEU ASP PRO SER GLY PRO GLU ARG
SEQRES   7 A  301  TYR ALA TYR ALA GLU HIS ARG ASP TYR LEU ASP ALA LEU
SEQRES   8 A  301  TRP GLU ALA LEU ASP LEU GLY ASP ARG VAL VAL LEU VAL
SEQRES   9 A  301  VAL HIS ASP TRP GLY SER ALA LEU GLY PHE ASP TRP ALA
SEQRES  10 A  301  ARG ARG HIS ARG GLU ARG VAL GLN GLY ILE ALA TYR MET
SEQRES  11 A  301  GLU ALA ILE ALA MET PRO ILE GLU ALA ALA ASP LEU PRO
SEQRES  12 A  301  GLU GLN ASP ARG ASP LEU PHE GLN ALA PHE ARG SER GLN
SEQRES  13 A  301  ALA GLY GLU GLU LEU VAL LEU GLN ASP ASN VAL PHE VAL
SEQRES  14 A  301  GLU GLN VAL LEU PRO GLY TRP ILE LEU ARG PRO LEU SER
SEQRES  15 A  301  GLU ALA GLU MET ALA ALA TYR ARG GLU PRO PHE LEU ALA
SEQRES  16 A  301  ALA GLY GLU ALA ARG ARG PRO THR LEU SER TRP PRO ARG
SEQRES  17 A  301  GLN LEU PRO ILE ALA GLY THR PRO ALA ASP VAL VAL ALA
SEQRES  18 A  301  ILE ALA ARG ASP TYR ALA GLY TRP LEU SER GLU SER PRO
SEQRES  19 A  301  ILE PRO LYS LEU PHE ILE ASN ALA GLU PRO GLY ALA LEU
SEQRES  20 A  301  THR THR GLY ARG MET ARG ASP PHE CYS ARG THR TRP PRO
SEQRES  21 A  301  ASN GLN THR GLU ILE THR VAL ALA GLY ALA HIS PHE ILE
SEQRES  22 A  301  GLN GLU ASP SER PRO ASP GLU ILE GLY ALA ALA ILE ALA
SEQRES  23 A  301  ALA PHE VAL ARG ARG LEU ARG PRO ALA HIS HIS HIS HIS
SEQRES  24 A  301  HIS HIS
HET    SCN  A 401       3
HETNAM     SCN THIOCYANATE ION
FORMUL   2  SCN    C N S 1-
FORMUL   3  HOH   *434(H2 O)
HELIX    1 AA1 SER A   41  ARG A   46  5                                   6
HELIX    2 AA2 ILE A   48  ALA A   53  5                                   6
HELIX    3 AA3 ALA A   81  LEU A   96  1                                  16
HELIX    4 AA4 TRP A  109  HIS A  121  1                                  13
HELIX    5 AA5 GLU A  139  LEU A  143  5                                   5
HELIX    6 AA6 PRO A  144  GLN A  146  5                                   3
HELIX    7 AA7 ASP A  147  SER A  156  1                                  10
HELIX    8 AA8 ALA A  158  GLN A  165  1                                   8
HELIX    9 AA9 ASN A  167  GLN A  172  1                                   6
HELIX   10 AB1 GLN A  172  TRP A  177  1                                   6
HELIX   11 AB2 SER A  183  GLU A  192  1                                  10
HELIX   12 AB3 PRO A  193  LEU A  195  5                                   3
HELIX   13 AB4 GLY A  198  ALA A  200  5                                   3
HELIX   14 AB5 ARG A  201  LEU A  211  1                                  11
HELIX   15 AB6 PRO A  217  SER A  232  1                                  16
HELIX   16 AB7 THR A  250  ARG A  258  1                                   9
HELIX   17 AB8 PHE A  273  ASP A  277  5                                   5
HELIX   18 AB9 SER A  278  ARG A  294  1                                  17
SHEET    1 AA1 8 LYS A  12  ILE A  16  0
SHEET    2 AA1 8 ARG A  19  GLU A  26 -1  O  ARG A  19   N  ILE A  16
SHEET    3 AA1 8 ARG A  57  CYS A  61 -1  O  LEU A  58   N  GLU A  26
SHEET    4 AA1 8 PRO A  31  GLN A  35  1  N  PHE A  34   O  ILE A  59
SHEET    5 AA1 8 VAL A 102  ASP A 108  1  O  VAL A 103   N  LEU A  33
SHEET    6 AA1 8 VAL A 125  ALA A 133  1  O  ALA A 129   N  LEU A 104
SHEET    7 AA1 8 LYS A 238  PRO A 245  1  O  ILE A 241   N  TYR A 130
SHEET    8 AA1 8 GLN A 263  GLY A 270  1  O  THR A 264   N  PHE A 240
CISPEP   1 ASN A   38    PRO A   39          0        -5.79
CISPEP   2 ASP A   73    PRO A   74          0        12.01
CISPEP   3 THR A  216    PRO A  217          0        -4.19
CISPEP   4 THR A  216    PRO A  217          0        -1.95
CISPEP   5 GLU A  244    PRO A  245          0         3.73
SITE     1 AC1  5 ASN A  38  ASP A 108  TRP A 109  TRP A 207
SITE     2 AC1  5 PRO A 208
CRYST1   46.867   68.362   80.648  90.00  90.00  90.00 P 21 21 21    4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.021337  0.000000  0.000000        0.00000
SCALE2      0.000000  0.014628  0.000000        0.00000
SCALE3      0.000000  0.000000  0.012400        0.00000
TER    2532      HIS A 302
MASTER      253    0    1   18    8    0    2    6 2810    1    3   24
END