longtext: 5y51-pdb

content
HEADER    HYDROLASE                               06-AUG-17   5Y51
TITLE     CRYSTAL STRUCTURE OF PYTH_H230A
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: PYRETHROID HYDROLASE;
COMPND   3 CHAIN: A, B, C, D, E, F;
COMPND   4 ENGINEERED: YES;
COMPND   5 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: SPHINGOBIUM FANIAE;
SOURCE   3 ORGANISM_TAXID: 570446;
SOURCE   4 GENE: PYTH;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    D.Q.XU,T.T.RAN,J.HE,W.W.WANG
REVDAT   1   08-AUG-18 5Y51    0
JRNL        AUTH   D.Q.XU,T.T.RAN,J.HE,W.W.WANG
JRNL        TITL   STRUCTURE AND CATALYTIC MECHANISM OF A NOVEL PYRETHROID
JRNL        TITL 2 HYDROLASE FROM SPHINGOBIUM FANIAE JZ-2
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.30 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (DEV_2247: ???)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.30
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.82
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.330
REMARK   3   COMPLETENESS FOR RANGE        (%) : 94.8
REMARK   3   NUMBER OF REFLECTIONS             : 74982
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.272
REMARK   3   R VALUE            (WORKING SET) : 0.269
REMARK   3   FREE R VALUE                     : 0.330
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.040
REMARK   3   FREE R VALUE TEST SET COUNT      : 3779
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 19.8163 -  6.8090    0.90     2736   123  0.1699 0.1890
REMARK   3     2  6.8090 -  5.4413    0.92     2632   141  0.2105 0.2559
REMARK   3     3  5.4413 -  4.7644    0.93     2647   132  0.2063 0.3111
REMARK   3     4  4.7644 -  4.3338    0.93     2634   125  0.1962 0.2358
REMARK   3     5  4.3338 -  4.0259    0.94     2633   150  0.2093 0.2793
REMARK   3     6  4.0259 -  3.7903    0.93     2606   151  0.2847 0.3437
REMARK   3     7  3.7903 -  3.6017    0.94     2618   132  0.3023 0.3120
REMARK   3     8  3.6017 -  3.4457    0.95     2610   156  0.2740 0.3454
REMARK   3     9  3.4457 -  3.3137    0.94     2607   145  0.3119 0.3521
REMARK   3    10  3.3137 -  3.1999    0.95     2626   157  0.2871 0.3580
REMARK   3    11  3.1999 -  3.1002    0.95     2670   123  0.2956 0.3750
REMARK   3    12  3.1002 -  3.0119    0.95     2637   126  0.2999 0.3686
REMARK   3    13  3.0119 -  2.9329    0.95     2629   145  0.3057 0.3674
REMARK   3    14  2.9329 -  2.8615    0.95     2637   133  0.3066 0.3579
REMARK   3    15  2.8615 -  2.7966    0.96     2632   136  0.3290 0.4863
REMARK   3    16  2.7966 -  2.7373    0.96     2643   151  0.3237 0.3417
REMARK   3    17  2.7373 -  2.6827    0.96     2611   139  0.3256 0.4487
REMARK   3    18  2.6827 -  2.6322    0.94     2593   131  0.3885 0.4077
REMARK   3    19  2.6322 -  2.5853    0.96     2615   164  0.3178 0.3461
REMARK   3    20  2.5853 -  2.5415    0.96     2631   156  0.3142 0.3719
REMARK   3    21  2.5415 -  2.5006    0.96     2633   153  0.3044 0.3523
REMARK   3    22  2.5006 -  2.4622    0.96     2656   123  0.3054 0.3909
REMARK   3    23  2.4622 -  2.4261    0.97     2666   141  0.3182 0.3993
REMARK   3    24  2.4261 -  2.3919    0.96     2683   120  0.3361 0.4048
REMARK   3    25  2.3919 -  2.3597    0.96     2614   155  0.3448 0.4319
REMARK   3    26  2.3597 -  2.3291    0.97     2667   129  0.3521 0.4587
REMARK   3    27  2.3291 -  2.3000    0.97     2637   142  0.3543 0.4572
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.430
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 36.980
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.011          11678
REMARK   3   ANGLE     :  1.145          15989
REMARK   3   CHIRALITY :  0.059           1801
REMARK   3   PLANARITY :  0.009           2129
REMARK   3   DIHEDRAL  : 18.555           6995
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 5Y51 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 07-AUG-17.
REMARK 100 THE DEPOSITION ID IS D_1300004673.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 15-OCT-13
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 7.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRF
REMARK 200  BEAMLINE                       : BL17U1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9793
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 75180
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.300
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 1.900
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 95.4
REMARK 200  DATA REDUNDANCY                : 7.700
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 8.0000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.30
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.42
REMARK 200  COMPLETENESS FOR SHELL     (%) : 97.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.70
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 1.900
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 47.80
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.36
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: AMMONIUM SULFATE, PH 7.5, VAPOR
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 42 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z
REMARK 290       3555   -Y+1/2,X+1/2,Z+1/2
REMARK 290       4555   Y+1/2,-X+1/2,Z+1/2
REMARK 290       5555   -X+1/2,Y+1/2,-Z+1/2
REMARK 290       6555   X+1/2,-Y+1/2,-Z+1/2
REMARK 290       7555   Y,X,-Z
REMARK 290       8555   -Y,-X,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       84.18750
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       84.18750
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       61.92900
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       84.18750
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       84.18750
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       61.92900
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       84.18750
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       84.18750
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       61.92900
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       84.18750
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       84.18750
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       61.92900
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4, 5, 6
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 5
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: E
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 6
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: F
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     GLU A   137
REMARK 465     GLY A   138
REMARK 465     GLY A   161
REMARK 465     GLU A   162
REMARK 465     GLY A   163
REMARK 465     ARG A   255
REMARK 465     GLN A   256
REMARK 465     THR A   257
REMARK 465     ALA A   258
REMARK 465     THR A   259
REMARK 465     LYS A   260
REMARK 465     ALA A   261
REMARK 465     GLY A   262
REMARK 465     PRO A   263
REMARK 465     ASP A   264
REMARK 465     ARG A   265
REMARK 465     PRO A   266
REMARK 465     ALA A   267
REMARK 465     GLY A   268
REMARK 465     ALA A   269
REMARK 465     ASP A   270
REMARK 465     GLY A   271
REMARK 465     GLY A   272
REMARK 465     ARG A   273
REMARK 465     ALA A   274
REMARK 465     ASP A   275
REMARK 465     ARG A   276
REMARK 465     ALA A   277
REMARK 465     ASP A   278
REMARK 465     LEU A   279
REMARK 465     PRO A   280
REMARK 465     LEU A   281
REMARK 465     GLU A   282
REMARK 465     HIS A   283
REMARK 465     HIS A   284
REMARK 465     HIS A   285
REMARK 465     HIS A   286
REMARK 465     HIS A   287
REMARK 465     HIS A   288
REMARK 465     MET B     1
REMARK 465     GLU B   137
REMARK 465     GLY B   138
REMARK 465     GLY B   161
REMARK 465     GLU B   162
REMARK 465     ARG B   255
REMARK 465     GLN B   256
REMARK 465     THR B   257
REMARK 465     ALA B   258
REMARK 465     THR B   259
REMARK 465     LYS B   260
REMARK 465     ALA B   261
REMARK 465     GLY B   262
REMARK 465     PRO B   263
REMARK 465     ASP B   264
REMARK 465     ARG B   265
REMARK 465     PRO B   266
REMARK 465     ALA B   267
REMARK 465     GLY B   268
REMARK 465     ALA B   269
REMARK 465     ASP B   270
REMARK 465     GLY B   271
REMARK 465     GLY B   272
REMARK 465     ARG B   273
REMARK 465     ALA B   274
REMARK 465     ASP B   275
REMARK 465     ARG B   276
REMARK 465     ALA B   277
REMARK 465     ASP B   278
REMARK 465     LEU B   279
REMARK 465     PRO B   280
REMARK 465     LEU B   281
REMARK 465     GLU B   282
REMARK 465     HIS B   283
REMARK 465     HIS B   284
REMARK 465     HIS B   285
REMARK 465     HIS B   286
REMARK 465     HIS B   287
REMARK 465     HIS B   288
REMARK 465     MET C     1
REMARK 465     GLU C   162
REMARK 465     GLY C   163
REMARK 465     ARG C   255
REMARK 465     GLN C   256
REMARK 465     THR C   257
REMARK 465     ALA C   258
REMARK 465     THR C   259
REMARK 465     LYS C   260
REMARK 465     ALA C   261
REMARK 465     GLY C   262
REMARK 465     PRO C   263
REMARK 465     ASP C   264
REMARK 465     ARG C   265
REMARK 465     PRO C   266
REMARK 465     ALA C   267
REMARK 465     GLY C   268
REMARK 465     ALA C   269
REMARK 465     ASP C   270
REMARK 465     GLY C   271
REMARK 465     GLY C   272
REMARK 465     ARG C   273
REMARK 465     ALA C   274
REMARK 465     ASP C   275
REMARK 465     ARG C   276
REMARK 465     ALA C   277
REMARK 465     ASP C   278
REMARK 465     LEU C   279
REMARK 465     PRO C   280
REMARK 465     LEU C   281
REMARK 465     GLU C   282
REMARK 465     HIS C   283
REMARK 465     HIS C   284
REMARK 465     HIS C   285
REMARK 465     HIS C   286
REMARK 465     HIS C   287
REMARK 465     HIS C   288
REMARK 465     MET D     1
REMARK 465     GLY D   161
REMARK 465     GLU D   162
REMARK 465     ARG D   255
REMARK 465     GLN D   256
REMARK 465     THR D   257
REMARK 465     ALA D   258
REMARK 465     THR D   259
REMARK 465     LYS D   260
REMARK 465     ALA D   261
REMARK 465     GLY D   262
REMARK 465     PRO D   263
REMARK 465     ASP D   264
REMARK 465     ARG D   265
REMARK 465     PRO D   266
REMARK 465     ALA D   267
REMARK 465     GLY D   268
REMARK 465     ALA D   269
REMARK 465     ASP D   270
REMARK 465     GLY D   271
REMARK 465     GLY D   272
REMARK 465     ARG D   273
REMARK 465     ALA D   274
REMARK 465     ASP D   275
REMARK 465     ARG D   276
REMARK 465     ALA D   277
REMARK 465     ASP D   278
REMARK 465     LEU D   279
REMARK 465     PRO D   280
REMARK 465     LEU D   281
REMARK 465     GLU D   282
REMARK 465     HIS D   283
REMARK 465     HIS D   284
REMARK 465     HIS D   285
REMARK 465     HIS D   286
REMARK 465     HIS D   287
REMARK 465     HIS D   288
REMARK 465     MET E     1
REMARK 465     GLY E   161
REMARK 465     GLU E   162
REMARK 465     GLY E   163
REMARK 465     ARG E   255
REMARK 465     GLN E   256
REMARK 465     THR E   257
REMARK 465     ALA E   258
REMARK 465     THR E   259
REMARK 465     LYS E   260
REMARK 465     ALA E   261
REMARK 465     GLY E   262
REMARK 465     PRO E   263
REMARK 465     ASP E   264
REMARK 465     ARG E   265
REMARK 465     PRO E   266
REMARK 465     ALA E   267
REMARK 465     GLY E   268
REMARK 465     ALA E   269
REMARK 465     ASP E   270
REMARK 465     GLY E   271
REMARK 465     GLY E   272
REMARK 465     ARG E   273
REMARK 465     ALA E   274
REMARK 465     ASP E   275
REMARK 465     ARG E   276
REMARK 465     ALA E   277
REMARK 465     ASP E   278
REMARK 465     LEU E   279
REMARK 465     PRO E   280
REMARK 465     LEU E   281
REMARK 465     GLU E   282
REMARK 465     HIS E   283
REMARK 465     HIS E   284
REMARK 465     HIS E   285
REMARK 465     HIS E   286
REMARK 465     HIS E   287
REMARK 465     HIS E   288
REMARK 465     MET F     1
REMARK 465     GLU F   137
REMARK 465     GLY F   138
REMARK 465     GLY F   161
REMARK 465     GLU F   162
REMARK 465     GLY F   163
REMARK 465     ARG F   255
REMARK 465     GLN F   256
REMARK 465     THR F   257
REMARK 465     ALA F   258
REMARK 465     THR F   259
REMARK 465     LYS F   260
REMARK 465     ALA F   261
REMARK 465     GLY F   262
REMARK 465     PRO F   263
REMARK 465     ASP F   264
REMARK 465     ARG F   265
REMARK 465     PRO F   266
REMARK 465     ALA F   267
REMARK 465     GLY F   268
REMARK 465     ALA F   269
REMARK 465     ASP F   270
REMARK 465     GLY F   271
REMARK 465     GLY F   272
REMARK 465     ARG F   273
REMARK 465     ALA F   274
REMARK 465     ASP F   275
REMARK 465     ARG F   276
REMARK 465     ALA F   277
REMARK 465     ASP F   278
REMARK 465     LEU F   279
REMARK 465     PRO F   280
REMARK 465     LEU F   281
REMARK 465     GLU F   282
REMARK 465     HIS F   283
REMARK 465     HIS F   284
REMARK 465     HIS F   285
REMARK 465     HIS F   286
REMARK 465     HIS F   287
REMARK 465     HIS F   288
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     ARG C 188    CG   CD   NE   CZ   NH1  NH2
REMARK 470     ARG D 188    CG   CD   NE   CZ   NH1  NH2
REMARK 470     LEU E 117    CG   CD1  CD2
REMARK 470     ARG E 188    CG   CD   NE   CZ   NH1  NH2
REMARK 470     LEU E 190    CG   CD1  CD2
REMARK 470     GLU E 191    CG   CD   OE1  OE2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OE2  GLU E   149     NH2  ARG E   152              1.87
REMARK 500   OH   TYR D    31     OD1  ASP D   249              2.09
REMARK 500   OH   TYR E    31     OD1  ASP E   249              2.11
REMARK 500   O    PRO E   121     NE2  GLN E   212              2.11
REMARK 500   NH1  ARG E   194     CE1  PHE E   217              2.13
REMARK 500   NE2  HIS F    41     OH   TYR F    57              2.13
REMARK 500   O    LEU A    49     NH1  ARG D   139              2.16
REMARK 500   OE1  GLU D    55     O    HOH D   401              2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    PRO D 160   C   -  N   -  CA  ANGL. DEV. =   9.0 DEGREES
REMARK 500    PRO F 134   C   -  N   -  CA  ANGL. DEV. =  10.4 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A  78     -111.21     42.56
REMARK 500    THR A 102       49.75     33.24
REMARK 500    SER A 229     -137.86   -100.45
REMARK 500    MET A 236       65.81   -150.09
REMARK 500    ALA A 250       81.22   -166.49
REMARK 500    SER B  78     -124.82     54.32
REMARK 500    THR B 102       45.63     32.27
REMARK 500    THR B 125       67.24   -112.15
REMARK 500    SER B 229     -138.79    -95.48
REMARK 500    MET B 236       68.98   -153.71
REMARK 500    ALA B 250       79.35   -161.01
REMARK 500    SER C  78     -122.70     52.70
REMARK 500    THR C 102       49.74     32.69
REMARK 500    ASP C 144       94.78    -69.36
REMARK 500    PRO C 160      105.45    -55.04
REMARK 500    PRO C 184       31.35    -94.55
REMARK 500    SER C 229     -150.48   -108.14
REMARK 500    MET C 236       65.07   -151.62
REMARK 500    ALA C 250       82.10   -159.53
REMARK 500    ASP D  53     -173.34   -170.13
REMARK 500    SER D  78     -130.49     50.75
REMARK 500    TYR D 100       71.45   -110.16
REMARK 500    THR D 102       48.63     33.51
REMARK 500    ASP D 136       65.22     63.58
REMARK 500    SER D 229     -153.01    -95.14
REMARK 500    ALA D 250       76.70   -174.16
REMARK 500    ALA E  12      133.06    -32.07
REMARK 500    PRO E  43      127.43    -33.59
REMARK 500    SER E  78     -120.95     54.75
REMARK 500    HIS E  91       49.15   -144.63
REMARK 500    THR E 102       59.83     33.02
REMARK 500    ASN E 122       74.02     42.86
REMARK 500    GLU E 168       -5.39    -55.45
REMARK 500    THR E 176       -9.08    -59.45
REMARK 500    LEU E 200      -31.87    -38.04
REMARK 500    PRO E 227       46.34    -80.49
REMARK 500    SER E 229     -140.06    -93.59
REMARK 500    TYR E 233       -9.36    -59.74
REMARK 500    ALA E 250       65.62   -158.94
REMARK 500    LEU F  13       12.78     59.31
REMARK 500    VAL F  52      -70.12    -80.59
REMARK 500    SER F  78     -116.14     60.27
REMARK 500    THR F 102       53.33     30.79
REMARK 500    PRO F 108      134.36    -39.78
REMARK 500    SER F 229     -135.64    -88.58
REMARK 500    ALA F 250       78.33   -167.07
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH E 404        DISTANCE =  7.59 ANGSTROMS
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 B 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 C 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 D 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 E 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 F 301
DBREF  5Y51 A    1   280  UNP    D0VUS3   D0VUS3_9SPHN     1    280
DBREF  5Y51 B    1   280  UNP    D0VUS3   D0VUS3_9SPHN     1    280
DBREF  5Y51 C    1   280  UNP    D0VUS3   D0VUS3_9SPHN     1    280
DBREF  5Y51 D    1   280  UNP    D0VUS3   D0VUS3_9SPHN     1    280
DBREF  5Y51 E    1   280  UNP    D0VUS3   D0VUS3_9SPHN     1    280
DBREF  5Y51 F    1   280  UNP    D0VUS3   D0VUS3_9SPHN     1    280
SEQADV 5Y51 ALA A  230  UNP  D0VUS3    HIS   230 ENGINEERED MUTATION
SEQADV 5Y51 LEU A  281  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 GLU A  282  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS A  283  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS A  284  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS A  285  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS A  286  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS A  287  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS A  288  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 ALA B  230  UNP  D0VUS3    HIS   230 ENGINEERED MUTATION
SEQADV 5Y51 LEU B  281  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 GLU B  282  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS B  283  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS B  284  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS B  285  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS B  286  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS B  287  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS B  288  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 ALA C  230  UNP  D0VUS3    HIS   230 ENGINEERED MUTATION
SEQADV 5Y51 LEU C  281  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 GLU C  282  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS C  283  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS C  284  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS C  285  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS C  286  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS C  287  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS C  288  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 ALA D  230  UNP  D0VUS3    HIS   230 ENGINEERED MUTATION
SEQADV 5Y51 LEU D  281  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 GLU D  282  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS D  283  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS D  284  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS D  285  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS D  286  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS D  287  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS D  288  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 ALA E  230  UNP  D0VUS3    HIS   230 ENGINEERED MUTATION
SEQADV 5Y51 LEU E  281  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 GLU E  282  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS E  283  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS E  284  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS E  285  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS E  286  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS E  287  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS E  288  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 ALA F  230  UNP  D0VUS3    HIS   230 ENGINEERED MUTATION
SEQADV 5Y51 LEU F  281  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 GLU F  282  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS F  283  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS F  284  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS F  285  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS F  286  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS F  287  UNP  D0VUS3              EXPRESSION TAG
SEQADV 5Y51 HIS F  288  UNP  D0VUS3              EXPRESSION TAG
SEQRES   1 A  288  MET THR VAL THR ASP ILE ILE LEU ILE HIS GLY ALA LEU
SEQRES   2 A  288  ASN ARG GLY ALA CYS TYR ASP ALA VAL VAL PRO LEU LEU
SEQRES   3 A  288  GLU ALA ARG GLY TYR ARG VAL HIS ALA PRO ASP LEU THR
SEQRES   4 A  288  GLY HIS THR PRO GLY ASP GLY GLY HIS LEU SER VAL VAL
SEQRES   5 A  288  ASP MET GLU HIS TYR THR ARG PRO VAL ALA ASP ILE LEU
SEQRES   6 A  288  ALA ARG ALA GLU GLY GLN SER ILE LEU LEU GLY HIS SER
SEQRES   7 A  288  LEU GLY GLY ALA SER ILE SER TRP LEU ALA GLN HIS HIS
SEQRES   8 A  288  PRO ASP LYS VAL ALA GLY LEU ILE TYR LEU THR ALA VAL
SEQRES   9 A  288  LEU THR ALA PRO GLY VAL THR PRO GLU THR PHE VAL LEU
SEQRES  10 A  288  PRO GLY GLU PRO ASN ARG GLY THR PRO HIS ALA LEU ASP
SEQRES  11 A  288  LEU ILE GLN PRO VAL ASP GLU GLY ARG GLY LEU GLN ALA
SEQRES  12 A  288  ASP PHE SER ARG LEU GLU ARG LEU ARG GLU VAL PHE MET
SEQRES  13 A  288  GLY ASP TYR PRO GLY GLU GLY MET PRO PRO ALA GLU HIS
SEQRES  14 A  288  PHE ILE GLN THR GLN SER THR VAL PRO PHE GLY THR PRO
SEQRES  15 A  288  ASN PRO MET GLU GLY ARG ALA LEU GLU ILE PRO ARG LEU
SEQRES  16 A  288  TYR ILE GLU ALA LEU ASP ASP VAL VAL LEU PRO ILE ALA
SEQRES  17 A  288  VAL GLN ARG GLN MET GLN LYS GLU PHE PRO GLY PRO VAL
SEQRES  18 A  288  ALA VAL VAL SER LEU PRO ALA SER ALA ALA PRO TYR TYR
SEQRES  19 A  288  SER MET PRO GLU ARG LEU ALA GLU ALA ILE ALA ASP PHE
SEQRES  20 A  288  ALA ASP ALA PRO ALA GLU TYR ARG GLN THR ALA THR LYS
SEQRES  21 A  288  ALA GLY PRO ASP ARG PRO ALA GLY ALA ASP GLY GLY ARG
SEQRES  22 A  288  ALA ASP ARG ALA ASP LEU PRO LEU GLU HIS HIS HIS HIS
SEQRES  23 A  288  HIS HIS
SEQRES   1 B  288  MET THR VAL THR ASP ILE ILE LEU ILE HIS GLY ALA LEU
SEQRES   2 B  288  ASN ARG GLY ALA CYS TYR ASP ALA VAL VAL PRO LEU LEU
SEQRES   3 B  288  GLU ALA ARG GLY TYR ARG VAL HIS ALA PRO ASP LEU THR
SEQRES   4 B  288  GLY HIS THR PRO GLY ASP GLY GLY HIS LEU SER VAL VAL
SEQRES   5 B  288  ASP MET GLU HIS TYR THR ARG PRO VAL ALA ASP ILE LEU
SEQRES   6 B  288  ALA ARG ALA GLU GLY GLN SER ILE LEU LEU GLY HIS SER
SEQRES   7 B  288  LEU GLY GLY ALA SER ILE SER TRP LEU ALA GLN HIS HIS
SEQRES   8 B  288  PRO ASP LYS VAL ALA GLY LEU ILE TYR LEU THR ALA VAL
SEQRES   9 B  288  LEU THR ALA PRO GLY VAL THR PRO GLU THR PHE VAL LEU
SEQRES  10 B  288  PRO GLY GLU PRO ASN ARG GLY THR PRO HIS ALA LEU ASP
SEQRES  11 B  288  LEU ILE GLN PRO VAL ASP GLU GLY ARG GLY LEU GLN ALA
SEQRES  12 B  288  ASP PHE SER ARG LEU GLU ARG LEU ARG GLU VAL PHE MET
SEQRES  13 B  288  GLY ASP TYR PRO GLY GLU GLY MET PRO PRO ALA GLU HIS
SEQRES  14 B  288  PHE ILE GLN THR GLN SER THR VAL PRO PHE GLY THR PRO
SEQRES  15 B  288  ASN PRO MET GLU GLY ARG ALA LEU GLU ILE PRO ARG LEU
SEQRES  16 B  288  TYR ILE GLU ALA LEU ASP ASP VAL VAL LEU PRO ILE ALA
SEQRES  17 B  288  VAL GLN ARG GLN MET GLN LYS GLU PHE PRO GLY PRO VAL
SEQRES  18 B  288  ALA VAL VAL SER LEU PRO ALA SER ALA ALA PRO TYR TYR
SEQRES  19 B  288  SER MET PRO GLU ARG LEU ALA GLU ALA ILE ALA ASP PHE
SEQRES  20 B  288  ALA ASP ALA PRO ALA GLU TYR ARG GLN THR ALA THR LYS
SEQRES  21 B  288  ALA GLY PRO ASP ARG PRO ALA GLY ALA ASP GLY GLY ARG
SEQRES  22 B  288  ALA ASP ARG ALA ASP LEU PRO LEU GLU HIS HIS HIS HIS
SEQRES  23 B  288  HIS HIS
SEQRES   1 C  288  MET THR VAL THR ASP ILE ILE LEU ILE HIS GLY ALA LEU
SEQRES   2 C  288  ASN ARG GLY ALA CYS TYR ASP ALA VAL VAL PRO LEU LEU
SEQRES   3 C  288  GLU ALA ARG GLY TYR ARG VAL HIS ALA PRO ASP LEU THR
SEQRES   4 C  288  GLY HIS THR PRO GLY ASP GLY GLY HIS LEU SER VAL VAL
SEQRES   5 C  288  ASP MET GLU HIS TYR THR ARG PRO VAL ALA ASP ILE LEU
SEQRES   6 C  288  ALA ARG ALA GLU GLY GLN SER ILE LEU LEU GLY HIS SER
SEQRES   7 C  288  LEU GLY GLY ALA SER ILE SER TRP LEU ALA GLN HIS HIS
SEQRES   8 C  288  PRO ASP LYS VAL ALA GLY LEU ILE TYR LEU THR ALA VAL
SEQRES   9 C  288  LEU THR ALA PRO GLY VAL THR PRO GLU THR PHE VAL LEU
SEQRES  10 C  288  PRO GLY GLU PRO ASN ARG GLY THR PRO HIS ALA LEU ASP
SEQRES  11 C  288  LEU ILE GLN PRO VAL ASP GLU GLY ARG GLY LEU GLN ALA
SEQRES  12 C  288  ASP PHE SER ARG LEU GLU ARG LEU ARG GLU VAL PHE MET
SEQRES  13 C  288  GLY ASP TYR PRO GLY GLU GLY MET PRO PRO ALA GLU HIS
SEQRES  14 C  288  PHE ILE GLN THR GLN SER THR VAL PRO PHE GLY THR PRO
SEQRES  15 C  288  ASN PRO MET GLU GLY ARG ALA LEU GLU ILE PRO ARG LEU
SEQRES  16 C  288  TYR ILE GLU ALA LEU ASP ASP VAL VAL LEU PRO ILE ALA
SEQRES  17 C  288  VAL GLN ARG GLN MET GLN LYS GLU PHE PRO GLY PRO VAL
SEQRES  18 C  288  ALA VAL VAL SER LEU PRO ALA SER ALA ALA PRO TYR TYR
SEQRES  19 C  288  SER MET PRO GLU ARG LEU ALA GLU ALA ILE ALA ASP PHE
SEQRES  20 C  288  ALA ASP ALA PRO ALA GLU TYR ARG GLN THR ALA THR LYS
SEQRES  21 C  288  ALA GLY PRO ASP ARG PRO ALA GLY ALA ASP GLY GLY ARG
SEQRES  22 C  288  ALA ASP ARG ALA ASP LEU PRO LEU GLU HIS HIS HIS HIS
SEQRES  23 C  288  HIS HIS
SEQRES   1 D  288  MET THR VAL THR ASP ILE ILE LEU ILE HIS GLY ALA LEU
SEQRES   2 D  288  ASN ARG GLY ALA CYS TYR ASP ALA VAL VAL PRO LEU LEU
SEQRES   3 D  288  GLU ALA ARG GLY TYR ARG VAL HIS ALA PRO ASP LEU THR
SEQRES   4 D  288  GLY HIS THR PRO GLY ASP GLY GLY HIS LEU SER VAL VAL
SEQRES   5 D  288  ASP MET GLU HIS TYR THR ARG PRO VAL ALA ASP ILE LEU
SEQRES   6 D  288  ALA ARG ALA GLU GLY GLN SER ILE LEU LEU GLY HIS SER
SEQRES   7 D  288  LEU GLY GLY ALA SER ILE SER TRP LEU ALA GLN HIS HIS
SEQRES   8 D  288  PRO ASP LYS VAL ALA GLY LEU ILE TYR LEU THR ALA VAL
SEQRES   9 D  288  LEU THR ALA PRO GLY VAL THR PRO GLU THR PHE VAL LEU
SEQRES  10 D  288  PRO GLY GLU PRO ASN ARG GLY THR PRO HIS ALA LEU ASP
SEQRES  11 D  288  LEU ILE GLN PRO VAL ASP GLU GLY ARG GLY LEU GLN ALA
SEQRES  12 D  288  ASP PHE SER ARG LEU GLU ARG LEU ARG GLU VAL PHE MET
SEQRES  13 D  288  GLY ASP TYR PRO GLY GLU GLY MET PRO PRO ALA GLU HIS
SEQRES  14 D  288  PHE ILE GLN THR GLN SER THR VAL PRO PHE GLY THR PRO
SEQRES  15 D  288  ASN PRO MET GLU GLY ARG ALA LEU GLU ILE PRO ARG LEU
SEQRES  16 D  288  TYR ILE GLU ALA LEU ASP ASP VAL VAL LEU PRO ILE ALA
SEQRES  17 D  288  VAL GLN ARG GLN MET GLN LYS GLU PHE PRO GLY PRO VAL
SEQRES  18 D  288  ALA VAL VAL SER LEU PRO ALA SER ALA ALA PRO TYR TYR
SEQRES  19 D  288  SER MET PRO GLU ARG LEU ALA GLU ALA ILE ALA ASP PHE
SEQRES  20 D  288  ALA ASP ALA PRO ALA GLU TYR ARG GLN THR ALA THR LYS
SEQRES  21 D  288  ALA GLY PRO ASP ARG PRO ALA GLY ALA ASP GLY GLY ARG
SEQRES  22 D  288  ALA ASP ARG ALA ASP LEU PRO LEU GLU HIS HIS HIS HIS
SEQRES  23 D  288  HIS HIS
SEQRES   1 E  288  MET THR VAL THR ASP ILE ILE LEU ILE HIS GLY ALA LEU
SEQRES   2 E  288  ASN ARG GLY ALA CYS TYR ASP ALA VAL VAL PRO LEU LEU
SEQRES   3 E  288  GLU ALA ARG GLY TYR ARG VAL HIS ALA PRO ASP LEU THR
SEQRES   4 E  288  GLY HIS THR PRO GLY ASP GLY GLY HIS LEU SER VAL VAL
SEQRES   5 E  288  ASP MET GLU HIS TYR THR ARG PRO VAL ALA ASP ILE LEU
SEQRES   6 E  288  ALA ARG ALA GLU GLY GLN SER ILE LEU LEU GLY HIS SER
SEQRES   7 E  288  LEU GLY GLY ALA SER ILE SER TRP LEU ALA GLN HIS HIS
SEQRES   8 E  288  PRO ASP LYS VAL ALA GLY LEU ILE TYR LEU THR ALA VAL
SEQRES   9 E  288  LEU THR ALA PRO GLY VAL THR PRO GLU THR PHE VAL LEU
SEQRES  10 E  288  PRO GLY GLU PRO ASN ARG GLY THR PRO HIS ALA LEU ASP
SEQRES  11 E  288  LEU ILE GLN PRO VAL ASP GLU GLY ARG GLY LEU GLN ALA
SEQRES  12 E  288  ASP PHE SER ARG LEU GLU ARG LEU ARG GLU VAL PHE MET
SEQRES  13 E  288  GLY ASP TYR PRO GLY GLU GLY MET PRO PRO ALA GLU HIS
SEQRES  14 E  288  PHE ILE GLN THR GLN SER THR VAL PRO PHE GLY THR PRO
SEQRES  15 E  288  ASN PRO MET GLU GLY ARG ALA LEU GLU ILE PRO ARG LEU
SEQRES  16 E  288  TYR ILE GLU ALA LEU ASP ASP VAL VAL LEU PRO ILE ALA
SEQRES  17 E  288  VAL GLN ARG GLN MET GLN LYS GLU PHE PRO GLY PRO VAL
SEQRES  18 E  288  ALA VAL VAL SER LEU PRO ALA SER ALA ALA PRO TYR TYR
SEQRES  19 E  288  SER MET PRO GLU ARG LEU ALA GLU ALA ILE ALA ASP PHE
SEQRES  20 E  288  ALA ASP ALA PRO ALA GLU TYR ARG GLN THR ALA THR LYS
SEQRES  21 E  288  ALA GLY PRO ASP ARG PRO ALA GLY ALA ASP GLY GLY ARG
SEQRES  22 E  288  ALA ASP ARG ALA ASP LEU PRO LEU GLU HIS HIS HIS HIS
SEQRES  23 E  288  HIS HIS
SEQRES   1 F  288  MET THR VAL THR ASP ILE ILE LEU ILE HIS GLY ALA LEU
SEQRES   2 F  288  ASN ARG GLY ALA CYS TYR ASP ALA VAL VAL PRO LEU LEU
SEQRES   3 F  288  GLU ALA ARG GLY TYR ARG VAL HIS ALA PRO ASP LEU THR
SEQRES   4 F  288  GLY HIS THR PRO GLY ASP GLY GLY HIS LEU SER VAL VAL
SEQRES   5 F  288  ASP MET GLU HIS TYR THR ARG PRO VAL ALA ASP ILE LEU
SEQRES   6 F  288  ALA ARG ALA GLU GLY GLN SER ILE LEU LEU GLY HIS SER
SEQRES   7 F  288  LEU GLY GLY ALA SER ILE SER TRP LEU ALA GLN HIS HIS
SEQRES   8 F  288  PRO ASP LYS VAL ALA GLY LEU ILE TYR LEU THR ALA VAL
SEQRES   9 F  288  LEU THR ALA PRO GLY VAL THR PRO GLU THR PHE VAL LEU
SEQRES  10 F  288  PRO GLY GLU PRO ASN ARG GLY THR PRO HIS ALA LEU ASP
SEQRES  11 F  288  LEU ILE GLN PRO VAL ASP GLU GLY ARG GLY LEU GLN ALA
SEQRES  12 F  288  ASP PHE SER ARG LEU GLU ARG LEU ARG GLU VAL PHE MET
SEQRES  13 F  288  GLY ASP TYR PRO GLY GLU GLY MET PRO PRO ALA GLU HIS
SEQRES  14 F  288  PHE ILE GLN THR GLN SER THR VAL PRO PHE GLY THR PRO
SEQRES  15 F  288  ASN PRO MET GLU GLY ARG ALA LEU GLU ILE PRO ARG LEU
SEQRES  16 F  288  TYR ILE GLU ALA LEU ASP ASP VAL VAL LEU PRO ILE ALA
SEQRES  17 F  288  VAL GLN ARG GLN MET GLN LYS GLU PHE PRO GLY PRO VAL
SEQRES  18 F  288  ALA VAL VAL SER LEU PRO ALA SER ALA ALA PRO TYR TYR
SEQRES  19 F  288  SER MET PRO GLU ARG LEU ALA GLU ALA ILE ALA ASP PHE
SEQRES  20 F  288  ALA ASP ALA PRO ALA GLU TYR ARG GLN THR ALA THR LYS
SEQRES  21 F  288  ALA GLY PRO ASP ARG PRO ALA GLY ALA ASP GLY GLY ARG
SEQRES  22 F  288  ALA ASP ARG ALA ASP LEU PRO LEU GLU HIS HIS HIS HIS
SEQRES  23 F  288  HIS HIS
HET    SO4  A 301       5
HET    SO4  B 301       5
HET    SO4  C 301       5
HET    SO4  D 301       5
HET    SO4  E 301       5
HET    SO4  F 301       5
HETNAM     SO4 SULFATE ION
FORMUL   7  SO4    6(O4 S 2-)
FORMUL  13  HOH   *33(H2 O)
HELIX    1 AA1 ARG A   15  ASP A   20  5                                   6
HELIX    2 AA2 VAL A   22  ARG A   29  1                                   8
HELIX    3 AA3 GLY A   47  VAL A   51  5                                   5
HELIX    4 AA4 ASP A   53  ALA A   68  1                                  16
HELIX    5 AA5 LEU A   79  HIS A   91  1                                  13
HELIX    6 AA6 PRO A  112  LEU A  117  1                                   6
HELIX    7 AA7 THR A  125  LEU A  131  1                                   7
HELIX    8 AA8 ARG A  147  MET A  156  1                                  10
HELIX    9 AA9 PRO A  166  PHE A  170  5                                   5
HELIX   10 AB1 THR A  176  THR A  181  1                                   6
HELIX   11 AB2 GLU A  186  ILE A  192  5                                   7
HELIX   12 AB3 PRO A  206  PHE A  217  1                                  12
HELIX   13 AB4 ALA A  231  MET A  236  1                                   6
HELIX   14 AB5 MET A  236  ALA A  250  1                                  15
HELIX   15 AB6 PRO A  251  TYR A  254  5                                   4
HELIX   16 AB7 ARG B   15  ASP B   20  5                                   6
HELIX   17 AB8 VAL B   22  ALA B   28  1                                   7
HELIX   18 AB9 GLY B   47  VAL B   51  5                                   5
HELIX   19 AC1 ASP B   53  THR B   58  1                                   6
HELIX   20 AC2 THR B   58  ALA B   66  1                                   9
HELIX   21 AC3 LEU B   79  HIS B   91  1                                  13
HELIX   22 AC4 PRO B  112  LEU B  117  1                                   6
HELIX   23 AC5 THR B  125  LEU B  131  1                                   7
HELIX   24 AC6 ARG B  147  MET B  156  1                                  10
HELIX   25 AC7 PRO B  166  PHE B  170  5                                   5
HELIX   26 AC8 VAL B  177  GLY B  180  5                                   4
HELIX   27 AC9 GLU B  186  ILE B  192  5                                   7
HELIX   28 AD1 PRO B  206  PHE B  217  1                                  12
HELIX   29 AD2 ALA B  231  MET B  236  1                                   6
HELIX   30 AD3 MET B  236  ALA B  250  1                                  15
HELIX   31 AD4 PRO B  251  TYR B  254  5                                   4
HELIX   32 AD5 ARG C   15  ASP C   20  5                                   6
HELIX   33 AD6 VAL C   22  ALA C   28  1                                   7
HELIX   34 AD7 GLY C   46  VAL C   51  5                                   6
HELIX   35 AD8 ASP C   53  THR C   58  1                                   6
HELIX   36 AD9 THR C   58  ALA C   68  1                                  11
HELIX   37 AE1 LEU C   79  HIS C   91  1                                  13
HELIX   38 AE2 PRO C  112  LEU C  117  1                                   6
HELIX   39 AE3 THR C  125  LEU C  131  1                                   7
HELIX   40 AE4 ARG C  147  MET C  156  1                                  10
HELIX   41 AE5 PRO C  166  PHE C  170  5                                   5
HELIX   42 AE6 THR C  176  THR C  181  1                                   6
HELIX   43 AE7 GLU C  186  ILE C  192  5                                   7
HELIX   44 AE8 PRO C  206  PHE C  217  1                                  12
HELIX   45 AE9 ALA C  231  MET C  236  1                                   6
HELIX   46 AF1 MET C  236  ALA C  250  1                                  15
HELIX   47 AF2 PRO C  251  TYR C  254  5                                   4
HELIX   48 AF3 ARG D   15  ASP D   20  5                                   6
HELIX   49 AF4 VAL D   22  ARG D   29  1                                   8
HELIX   50 AF5 GLY D   47  VAL D   51  5                                   5
HELIX   51 AF6 ASP D   53  THR D   58  1                                   6
HELIX   52 AF7 THR D   58  ALA D   68  1                                  11
HELIX   53 AF8 LEU D   79  HIS D   91  1                                  13
HELIX   54 AF9 PRO D  112  LEU D  117  1                                   6
HELIX   55 AG1 THR D  125  LEU D  131  1                                   7
HELIX   56 AG2 ARG D  147  MET D  156  1                                  10
HELIX   57 AG3 PRO D  166  PHE D  170  5                                   5
HELIX   58 AG4 VAL D  177  GLY D  180  5                                   4
HELIX   59 AG5 GLU D  186  ILE D  192  5                                   7
HELIX   60 AG6 PRO D  206  PHE D  217  1                                  12
HELIX   61 AG7 ALA D  231  MET D  236  1                                   6
HELIX   62 AG8 MET D  236  ASP D  249  1                                  14
HELIX   63 AG9 ARG E   15  ASP E   20  5                                   6
HELIX   64 AH1 VAL E   22  ALA E   28  1                                   7
HELIX   65 AH2 GLY E   47  VAL E   51  5                                   5
HELIX   66 AH3 ASP E   53  THR E   58  1                                   6
HELIX   67 AH4 THR E   58  ARG E   67  1                                  10
HELIX   68 AH5 GLY E   80  HIS E   91  1                                  12
HELIX   69 AH6 PRO E  112  LEU E  117  1                                   6
HELIX   70 AH7 THR E  125  LEU E  131  1                                   7
HELIX   71 AH8 ARG E  147  PHE E  155  1                                   9
HELIX   72 AH9 PRO E  166  PHE E  170  5                                   5
HELIX   73 AI1 THR E  176  THR E  181  1                                   6
HELIX   74 AI2 GLU E  186  ILE E  192  5                                   7
HELIX   75 AI3 PRO E  206  LYS E  215  1                                  10
HELIX   76 AI4 ALA E  231  MET E  236  1                                   6
HELIX   77 AI5 MET E  236  ASP E  249  1                                  14
HELIX   78 AI6 ALA E  250  TYR E  254  5                                   5
HELIX   79 AI7 ARG F   15  ASP F   20  5                                   6
HELIX   80 AI8 VAL F   22  ALA F   28  1                                   7
HELIX   81 AI9 GLY F   47  VAL F   51  5                                   5
HELIX   82 AJ1 ASP F   53  ALA F   68  1                                  16
HELIX   83 AJ2 LEU F   79  HIS F   91  1                                  13
HELIX   84 AJ3 PRO F  112  LEU F  117  1                                   6
HELIX   85 AJ4 THR F  125  LEU F  131  1                                   7
HELIX   86 AJ5 ARG F  147  MET F  156  1                                  10
HELIX   87 AJ6 PRO F  166  PHE F  170  5                                   5
HELIX   88 AJ7 THR F  176  THR F  181  1                                   6
HELIX   89 AJ8 GLU F  186  LEU F  190  5                                   5
HELIX   90 AJ9 PRO F  206  GLU F  216  1                                  11
HELIX   91 AK1 ALA F  231  MET F  236  1                                   6
HELIX   92 AK2 MET F  236  ALA F  250  1                                  15
HELIX   93 AK3 PRO F  251  TYR F  254  5                                   4
SHEET    1 AA1 6 ARG A  32  HIS A  34  0
SHEET    2 AA1 6 VAL A   3  ILE A   9  1  N  LEU A   8   O  HIS A  34
SHEET    3 AA1 6 GLN A  71  HIS A  77  1  O  GLN A  71   N  THR A   4
SHEET    4 AA1 6 VAL A  95  LEU A 101  1  O  ILE A  99   N  LEU A  74
SHEET    5 AA1 6 ARG A 194  ALA A 199  1  O  LEU A 195   N  TYR A 100
SHEET    6 AA1 6 ALA A 222  LEU A 226  1  O  LEU A 226   N  GLU A 198
SHEET    1 AA2 3 ILE A 132  VAL A 135  0
SHEET    2 AA2 3 GLY A 140  ALA A 143 -1  O  GLN A 142   N  GLN A 133
SHEET    3 AA2 3 GLN A 174  SER A 175 -1  O  GLN A 174   N  LEU A 141
SHEET    1 AA3 6 ARG B  32  HIS B  34  0
SHEET    2 AA3 6 VAL B   3  ILE B   9  1  N  ILE B   6   O  HIS B  34
SHEET    3 AA3 6 GLN B  71  HIS B  77  1  O  LEU B  75   N  ILE B   7
SHEET    4 AA3 6 VAL B  95  LEU B 101  1  O  ILE B  99   N  GLY B  76
SHEET    5 AA3 6 ARG B 194  ALA B 199  1  O  LEU B 195   N  LEU B  98
SHEET    6 AA3 6 ALA B 222  LEU B 226  1  O  LEU B 226   N  GLU B 198
SHEET    1 AA4 3 ILE B 132  VAL B 135  0
SHEET    2 AA4 3 GLY B 140  ALA B 143 -1  O  GLN B 142   N  GLN B 133
SHEET    3 AA4 3 GLN B 174  SER B 175 -1  O  GLN B 174   N  LEU B 141
SHEET    1 AA5 6 ARG C  32  HIS C  34  0
SHEET    2 AA5 6 VAL C   3  ILE C   9  1  N  LEU C   8   O  HIS C  34
SHEET    3 AA5 6 GLN C  71  HIS C  77  1  O  LEU C  75   N  ILE C   7
SHEET    4 AA5 6 VAL C  95  LEU C 101  1  O  ALA C  96   N  SER C  72
SHEET    5 AA5 6 ARG C 194  ALA C 199  1  O  LEU C 195   N  TYR C 100
SHEET    6 AA5 6 ALA C 222  LEU C 226  1  O  LEU C 226   N  GLU C 198
SHEET    1 AA6 3 ILE C 132  VAL C 135  0
SHEET    2 AA6 3 GLY C 140  ALA C 143 -1  O  GLY C 140   N  VAL C 135
SHEET    3 AA6 3 GLN C 174  SER C 175 -1  O  GLN C 174   N  LEU C 141
SHEET    1 AA7 6 ARG D  32  HIS D  34  0
SHEET    2 AA7 6 VAL D   3  ILE D   9  1  N  LEU D   8   O  HIS D  34
SHEET    3 AA7 6 GLN D  71  HIS D  77  1  O  ILE D  73   N  ILE D   7
SHEET    4 AA7 6 VAL D  95  LEU D 101  1  O  ILE D  99   N  GLY D  76
SHEET    5 AA7 6 ARG D 194  ALA D 199  1  O  LEU D 195   N  TYR D 100
SHEET    6 AA7 6 ALA D 222  LEU D 226  1  O  ALA D 222   N  TYR D 196
SHEET    1 AA8 3 ILE D 132  VAL D 135  0
SHEET    2 AA8 3 GLY D 140  ALA D 143 -1  O  GLY D 140   N  VAL D 135
SHEET    3 AA8 3 GLN D 174  SER D 175 -1  O  GLN D 174   N  LEU D 141
SHEET    1 AA9 6 ARG E  32  HIS E  34  0
SHEET    2 AA9 6 VAL E   3  ILE E   9  1  N  ILE E   6   O  ARG E  32
SHEET    3 AA9 6 GLN E  71  HIS E  77  1  O  GLN E  71   N  THR E   4
SHEET    4 AA9 6 VAL E  95  LEU E 101  1  O  ILE E  99   N  GLY E  76
SHEET    5 AA9 6 ARG E 194  ALA E 199  1  O  ILE E 197   N  TYR E 100
SHEET    6 AA9 6 ALA E 222  LEU E 226  1  O  ALA E 222   N  TYR E 196
SHEET    1 AB1 3 ILE E 132  VAL E 135  0
SHEET    2 AB1 3 GLY E 140  ALA E 143 -1  O  GLY E 140   N  VAL E 135
SHEET    3 AB1 3 THR E 173  SER E 175 -1  O  GLN E 174   N  LEU E 141
SHEET    1 AB2 6 ARG F  32  HIS F  34  0
SHEET    2 AB2 6 VAL F   3  ILE F   9  1  N  LEU F   8   O  HIS F  34
SHEET    3 AB2 6 GLN F  71  HIS F  77  1  O  GLN F  71   N  THR F   4
SHEET    4 AB2 6 VAL F  95  LEU F 101  1  O  LEU F 101   N  GLY F  76
SHEET    5 AB2 6 ARG F 194  ALA F 199  1  O  LEU F 195   N  TYR F 100
SHEET    6 AB2 6 ALA F 222  LEU F 226  1  O  ALA F 222   N  TYR F 196
SHEET    1 AB3 3 ILE F 132  VAL F 135  0
SHEET    2 AB3 3 GLY F 140  ALA F 143 -1  O  GLY F 140   N  VAL F 135
SHEET    3 AB3 3 GLN F 174  SER F 175 -1  O  GLN F 174   N  LEU F 141
CISPEP   1 GLU A  120    PRO A  121          0        -3.64
CISPEP   2 GLU B  120    PRO B  121          0        -2.43
CISPEP   3 GLU C  120    PRO C  121          0        -6.37
CISPEP   4 GLU D  120    PRO D  121          0         2.52
CISPEP   5 GLU E  120    PRO E  121          0       -10.43
CISPEP   6 GLU F  120    PRO F  121          0         2.66
SITE     1 AC1  5 SER A 235  MET A 236  PRO A 237  GLU A 238
SITE     2 AC1  5 ARG A 239
SITE     1 AC2  5 SER B 235  MET B 236  PRO B 237  GLU B 238
SITE     2 AC2  5 ARG B 239
SITE     1 AC3  5 SER C 235  MET C 236  PRO C 237  GLU C 238
SITE     2 AC3  5 ARG C 239
SITE     1 AC4  5 SER D 235  MET D 236  PRO D 237  GLU D 238
SITE     2 AC4  5 ARG D 239
SITE     1 AC5  5 SER E 235  MET E 236  PRO E 237  GLU E 238
SITE     2 AC5  5 ARG E 239
SITE     1 AC6  4 MET F 236  PRO F 237  GLU F 238  ARG F 239
CRYST1  168.375  168.375  123.858  90.00  90.00  90.00 P 42 21 2    48
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.005939  0.000000  0.000000        0.00000
SCALE2      0.000000  0.005939  0.000000        0.00000
SCALE3      0.000000  0.000000  0.008074        0.00000
TER    1889      TYR A 254
TER    3782      TYR B 254
TER    5682      TYR C 254
TER    7582      TYR D 254
TER    9468      TYR E 254
TER   11357      TYR F 254
MASTER      652    0    6   93   54    0   11    611414    6   30  138
END