longtext: 6eb3-pdb

content
HEADER    HYDROLASE                               03-AUG-18   6EB3
TITLE     STRUCTURAL AND ENZYMATIC CHARACTERIZATION OF AN ESTERASE FROM A
TITLE    2 METAGENOMIC LIBRARY
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: EST1;
COMPND   3 CHAIN: A, B, C, D
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: METAGENOME;
SOURCE   3 ORGANISM_TAXID: 256318
KEYWDS    APHA-BETA HYDROLASE, ESTERASE, METAGENOMIC, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    C.R.GUZZO,C.F.CARVALHO,R.D.TEIXEIRA,C.S.FARAH
REVDAT   1   07-AUG-19 6EB3    0
JRNL        AUTH   C.R.GUZZO,N.K.MACIEL,A.S.BARBOSA,C.S.FARAH
JRNL        TITL   FUNCTIONAL AND STRUCTURAL CHARACTERISATION OF AN ESTERASE
JRNL        TITL 2 FROM AMAZONIAN DARK SOIL
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.35 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.8.0189
REMARK   3   AUTHORS     : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK   3               : NICHOLLS,WINN,LONG,VAGIN
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.35
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.87
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.9
REMARK   3   NUMBER OF REFLECTIONS             : 44300
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.195
REMARK   3   R VALUE            (WORKING SET) : 0.193
REMARK   3   FREE R VALUE                     : 0.233
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.900
REMARK   3   FREE R VALUE TEST SET COUNT      : 2268
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.35
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.41
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 3227
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 100.0
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2630
REMARK   3   BIN FREE R VALUE SET COUNT          : 167
REMARK   3   BIN FREE R VALUE                    : 0.3260
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 8189
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 100
REMARK   3   SOLVENT ATOMS            : 391
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 43.39
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : -0.03000
REMARK   3    B22 (A**2) : 0.05000
REMARK   3    B33 (A**2) : -0.02000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.454
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.249
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.186
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 15.418
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.950
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.931
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  8593 ; 0.011 ; 0.019
REMARK   3   BOND LENGTHS OTHERS               (A):  8194 ; 0.002 ; 0.020
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 11666 ; 1.549 ; 1.980
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 19006 ; 0.983 ; 3.000
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  1085 ; 5.721 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   358 ;36.191 ;23.631
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1423 ;15.867 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    53 ;19.278 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1319 ; 0.089 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  9470 ; 0.007 ; 0.021
REMARK   3   GENERAL PLANES OTHERS             (A):  1683 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  4287 ; 1.604 ; 3.510
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  4286 ; 1.603 ; 3.510
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  5355 ; 2.621 ; 5.256
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  5356 ; 2.621 ; 5.256
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  4306 ; 1.984 ; 3.871
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  4303 ; 1.978 ; 3.866
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  6294 ; 3.287 ; 5.667
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  9197 ; 5.002 ;41.603
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  9142 ; 4.972 ;41.499
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NCS TYPE: LOCAL
REMARK   3   NUMBER OF DIFFERENT NCS PAIRS  : 6
REMARK   3  GROUP  CHAIN1    RANGE     CHAIN2     RANGE    COUNT RMS  WEIGHT
REMARK   3    1     A     1    268       B     1    268   16248  0.10  0.05
REMARK   3    2     A     1    268       C     1    268   16396  0.09  0.05
REMARK   3    3     A     1    268       D     1    268   16300  0.10  0.05
REMARK   3    4     B     1    268       C     1    268   16372  0.09  0.05
REMARK   3    5     B     1    268       D     1    268   16842  0.09  0.05
REMARK   3    6     C     1    268       D     1    268   16384  0.08  0.05
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 4
REMARK   3
REMARK   3   TLS GROUP : 1
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A     1        A   268
REMARK   3    ORIGIN FOR THE GROUP (A):  54.2395  58.8029  82.5095
REMARK   3    T TENSOR
REMARK   3      T11:   0.0365 T22:   0.1076
REMARK   3      T33:   0.0416 T12:  -0.0021
REMARK   3      T13:   0.0140 T23:  -0.0280
REMARK   3    L TENSOR
REMARK   3      L11:   0.8109 L22:   0.1952
REMARK   3      L33:   2.2240 L12:   0.0848
REMARK   3      L13:  -0.4747 L23:  -0.4994
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0840 S12:   0.0874 S13:   0.0631
REMARK   3      S21:   0.0060 S22:   0.1563 S23:  -0.0365
REMARK   3      S31:  -0.1254 S32:  -0.3563 S33:  -0.0723
REMARK   3
REMARK   3   TLS GROUP : 2
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B     1        B   268
REMARK   3    ORIGIN FOR THE GROUP (A):  14.8350  70.8010 136.6948
REMARK   3    T TENSOR
REMARK   3      T11:   0.0321 T22:   0.0434
REMARK   3      T33:   0.0798 T12:   0.0096
REMARK   3      T13:   0.0061 T23:   0.0368
REMARK   3    L TENSOR
REMARK   3      L11:   0.0342 L22:   0.9139
REMARK   3      L33:   1.7135 L12:  -0.1099
REMARK   3      L13:  -0.0078 L23:  -0.8428
REMARK   3    S TENSOR
REMARK   3      S11:   0.0195 S12:  -0.0271 S13:  -0.0404
REMARK   3      S21:   0.0774 S22:   0.1236 S23:   0.1938
REMARK   3      S31:  -0.1464 S32:   0.0260 S33:  -0.1431
REMARK   3
REMARK   3   TLS GROUP : 3
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   C     1        C   268
REMARK   3    ORIGIN FOR THE GROUP (A):  25.1081  90.9564  99.4285
REMARK   3    T TENSOR
REMARK   3      T11:   0.0263 T22:   0.0090
REMARK   3      T33:   0.0708 T12:  -0.0125
REMARK   3      T13:   0.0080 T23:   0.0064
REMARK   3    L TENSOR
REMARK   3      L11:   0.6570 L22:   0.0744
REMARK   3      L33:   0.8378 L12:   0.1599
REMARK   3      L13:   0.2236 L23:   0.1317
REMARK   3    S TENSOR
REMARK   3      S11:   0.0482 S12:   0.0094 S13:   0.0896
REMARK   3      S21:  -0.0085 S22:   0.0196 S23:   0.0225
REMARK   3      S31:   0.0397 S32:  -0.0011 S33:  -0.0679
REMARK   3
REMARK   3   TLS GROUP : 4
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   D     1        D   268
REMARK   3    ORIGIN FOR THE GROUP (A):  16.1863  53.7451 101.2803
REMARK   3    T TENSOR
REMARK   3      T11:   0.0354 T22:   0.0160
REMARK   3      T33:   0.0524 T12:  -0.0031
REMARK   3      T13:  -0.0069 T23:  -0.0153
REMARK   3    L TENSOR
REMARK   3      L11:   0.5280 L22:   0.4104
REMARK   3      L33:   0.3627 L12:  -0.0131
REMARK   3      L13:   0.0813 L23:  -0.0396
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0522 S12:   0.0100 S13:   0.0178
REMARK   3      S21:   0.0141 S22:   0.0465 S23:   0.0333
REMARK   3      S31:   0.0308 S32:   0.0075 S33:   0.0057
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING
REMARK   3  POSITIONS
REMARK   4
REMARK   4 6EB3 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 06-AUG-18.
REMARK 100 THE DEPOSITION ID IS D_1000235953.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 14-MAR-14
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 6.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : N
REMARK 200  RADIATION SOURCE               : ROTATING ANODE
REMARK 200  BEAMLINE                       : NULL
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-007 HF
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU RAXIS IV++
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MOSFLM
REMARK 200  DATA SCALING SOFTWARE          : SCALA
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 50655
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.300
REMARK 200  RESOLUTION RANGE LOW       (A) : 30.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9
REMARK 200  DATA REDUNDANCY                : 5.700
REMARK 200  R MERGE                    (I) : 0.10000
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 13.4000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.30
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.38
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 5.90
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 2.200
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: 2XUA
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 48.14
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.37
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 BIS-TRIS, PH 6.5, 0.1 M NACL, 1.3
REMARK 280  M (NH4)2SO4, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       35.82500
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       72.97000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       52.97500
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       72.97000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       35.82500
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       52.97500
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     GLU A   138
REMARK 465     GLY A   139
REMARK 465     ARG A   140
REMARK 465     GLU C   138
REMARK 465     GLY C   139
REMARK 465     ARG C   140
REMARK 465     ALA C   141
REMARK 465     GLY C   142
REMARK 465     LEU C   143
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     LYS A 135    CG   CD   CE   NZ
REMARK 470     LEU A 137    CG   CD1  CD2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ARG A  55   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.1 DEGREES
REMARK 500    PRO A 128   C   -  N   -  CA  ANGL. DEV. =  -9.3 DEGREES
REMARK 500    ARG A 252   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES
REMARK 500    ARG A 265   NE  -  CZ  -  NH1 ANGL. DEV. =   4.2 DEGREES
REMARK 500    ARG A 265   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.6 DEGREES
REMARK 500    ARG B 252   NE  -  CZ  -  NH1 ANGL. DEV. =   4.1 DEGREES
REMARK 500    ARG B 252   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.6 DEGREES
REMARK 500    ARG B 265   NE  -  CZ  -  NH1 ANGL. DEV. =   3.9 DEGREES
REMARK 500    ARG B 265   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.5 DEGREES
REMARK 500    ARG C 175   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES
REMARK 500    ARG C 175   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.0 DEGREES
REMARK 500    ARG C 252   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.5 DEGREES
REMARK 500    ARG D  55   NE  -  CZ  -  NH1 ANGL. DEV. =   4.2 DEGREES
REMARK 500    ARG D  55   NE  -  CZ  -  NH2 ANGL. DEV. =  -5.8 DEGREES
REMARK 500    ARG D 252   NE  -  CZ  -  NH1 ANGL. DEV. =   3.9 DEGREES
REMARK 500    ARG D 252   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.9 DEGREES
REMARK 500    ARG D 265   NE  -  CZ  -  NH1 ANGL. DEV. =   3.7 DEGREES
REMARK 500    ARG D 265   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.1 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A  94     -125.33     59.66
REMARK 500    CYS A 118       57.17     30.12
REMARK 500    LEU A 134       53.02    -64.70
REMARK 500    ALA A 136       46.75    -88.68
REMARK 500    SER A 242       53.06   -101.90
REMARK 500    SER B  94     -124.37     60.06
REMARK 500    CYS B 118       57.32     29.93
REMARK 500    SER B 242       54.43   -103.99
REMARK 500    SER C  94     -125.36     59.56
REMARK 500    CYS C 118       57.71     29.80
REMARK 500    SER D  94     -125.30     61.46
REMARK 500    CYS D 118       56.95     30.38
REMARK 500
REMARK 500 REMARK: NULL
REMARK 610
REMARK 610 MISSING HETEROATOM
REMARK 610 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 610 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 610 I=INSERTION CODE):
REMARK 610   M RES C SSEQI
REMARK 610     ABU C  301
REMARK 610     ABU D  301
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue J3J A 304
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue J3G B 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 B 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue ABU C 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 C 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 C 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 304
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 305
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue ABU D 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 D 302
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 D 303
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL D 304
DBREF  6EB3 A    1   268  PDB    6EB3     6EB3             1    268
DBREF  6EB3 B    1   268  PDB    6EB3     6EB3             1    268
DBREF  6EB3 C    1   268  PDB    6EB3     6EB3             1    268
DBREF  6EB3 D    1   268  PDB    6EB3     6EB3             1    268
SEQRES   1 A  268  MET LEU TYR ALA GLN VAL ASN GLY ILE ASN LEU HIS TYR
SEQRES   2 A  268  GLU ILE GLU GLY GLN GLY GLN PRO LEU LEU LEU ILE MET
SEQRES   3 A  268  GLY LEU GLY ALA PRO ALA ALA ALA TRP ASP PRO ILE PHE
SEQRES   4 A  268  VAL GLN THR LEU THR LYS THR HIS GLN VAL ILE ILE TYR
SEQRES   5 A  268  ASP ASN ARG GLY THR GLY LEU SER ASP LYS PRO ASP MET
SEQRES   6 A  268  PRO TYR SER ILE ALA MET PHE ALA SER ASP ALA VAL GLY
SEQRES   7 A  268  LEU LEU ASP ALA LEU ASN ILE PRO ARG ALA HIS VAL PHE
SEQRES   8 A  268  GLY VAL SER MET GLY GLY MET ILE ALA GLN GLU LEU ALA
SEQRES   9 A  268  ILE HIS TYR PRO GLN ARG VAL ALA SER LEU ILE LEU GLY
SEQRES  10 A  268  CYS THR THR PRO GLY GLY LYS HIS ALA VAL PRO ALA PRO
SEQRES  11 A  268  PRO GLU SER LEU LYS ALA LEU GLU GLY ARG ALA GLY LEU
SEQRES  12 A  268  THR PRO GLU GLU ALA ILE ARG GLU GLY TRP LYS LEU SER
SEQRES  13 A  268  PHE SER GLU GLU PHE ILE HIS THR HIS LYS ALA GLU LEU
SEQRES  14 A  268  GLU ALA HIS ILE PRO ARG LEU LEU ALA GLN LEU THR PRO
SEQRES  15 A  268  ARG PHE ALA TYR GLU ARG HIS PHE GLN ALA THR MET THR
SEQRES  16 A  268  LEU ARG VAL PHE LYS GLN LEU LYS GLU ILE GLN ALA PRO
SEQRES  17 A  268  THR LEU VAL ALA THR GLY ARG ASP ASP MET LEU ILE PRO
SEQRES  18 A  268  ALA VAL ASN SER GLU ILE LEU ALA ARG GLU ILE PRO GLY
SEQRES  19 A  268  ALA GLU LEU ALA ILE PHE GLU SER ALA GLY HIS GLY PHE
SEQRES  20 A  268  VAL THR SER ALA ARG GLU PRO PHE LEU LYS VAL LEU LYS
SEQRES  21 A  268  GLU PHE LEU ALA ARG GLN SER VAL
SEQRES   1 B  268  MET LEU TYR ALA GLN VAL ASN GLY ILE ASN LEU HIS TYR
SEQRES   2 B  268  GLU ILE GLU GLY GLN GLY GLN PRO LEU LEU LEU ILE MET
SEQRES   3 B  268  GLY LEU GLY ALA PRO ALA ALA ALA TRP ASP PRO ILE PHE
SEQRES   4 B  268  VAL GLN THR LEU THR LYS THR HIS GLN VAL ILE ILE TYR
SEQRES   5 B  268  ASP ASN ARG GLY THR GLY LEU SER ASP LYS PRO ASP MET
SEQRES   6 B  268  PRO TYR SER ILE ALA MET PHE ALA SER ASP ALA VAL GLY
SEQRES   7 B  268  LEU LEU ASP ALA LEU ASN ILE PRO ARG ALA HIS VAL PHE
SEQRES   8 B  268  GLY VAL SER MET GLY GLY MET ILE ALA GLN GLU LEU ALA
SEQRES   9 B  268  ILE HIS TYR PRO GLN ARG VAL ALA SER LEU ILE LEU GLY
SEQRES  10 B  268  CYS THR THR PRO GLY GLY LYS HIS ALA VAL PRO ALA PRO
SEQRES  11 B  268  PRO GLU SER LEU LYS ALA LEU GLU GLY ARG ALA GLY LEU
SEQRES  12 B  268  THR PRO GLU GLU ALA ILE ARG GLU GLY TRP LYS LEU SER
SEQRES  13 B  268  PHE SER GLU GLU PHE ILE HIS THR HIS LYS ALA GLU LEU
SEQRES  14 B  268  GLU ALA HIS ILE PRO ARG LEU LEU ALA GLN LEU THR PRO
SEQRES  15 B  268  ARG PHE ALA TYR GLU ARG HIS PHE GLN ALA THR MET THR
SEQRES  16 B  268  LEU ARG VAL PHE LYS GLN LEU LYS GLU ILE GLN ALA PRO
SEQRES  17 B  268  THR LEU VAL ALA THR GLY ARG ASP ASP MET LEU ILE PRO
SEQRES  18 B  268  ALA VAL ASN SER GLU ILE LEU ALA ARG GLU ILE PRO GLY
SEQRES  19 B  268  ALA GLU LEU ALA ILE PHE GLU SER ALA GLY HIS GLY PHE
SEQRES  20 B  268  VAL THR SER ALA ARG GLU PRO PHE LEU LYS VAL LEU LYS
SEQRES  21 B  268  GLU PHE LEU ALA ARG GLN SER VAL
SEQRES   1 C  268  MET LEU TYR ALA GLN VAL ASN GLY ILE ASN LEU HIS TYR
SEQRES   2 C  268  GLU ILE GLU GLY GLN GLY GLN PRO LEU LEU LEU ILE MET
SEQRES   3 C  268  GLY LEU GLY ALA PRO ALA ALA ALA TRP ASP PRO ILE PHE
SEQRES   4 C  268  VAL GLN THR LEU THR LYS THR HIS GLN VAL ILE ILE TYR
SEQRES   5 C  268  ASP ASN ARG GLY THR GLY LEU SER ASP LYS PRO ASP MET
SEQRES   6 C  268  PRO TYR SER ILE ALA MET PHE ALA SER ASP ALA VAL GLY
SEQRES   7 C  268  LEU LEU ASP ALA LEU ASN ILE PRO ARG ALA HIS VAL PHE
SEQRES   8 C  268  GLY VAL SER MET GLY GLY MET ILE ALA GLN GLU LEU ALA
SEQRES   9 C  268  ILE HIS TYR PRO GLN ARG VAL ALA SER LEU ILE LEU GLY
SEQRES  10 C  268  CYS THR THR PRO GLY GLY LYS HIS ALA VAL PRO ALA PRO
SEQRES  11 C  268  PRO GLU SER LEU LYS ALA LEU GLU GLY ARG ALA GLY LEU
SEQRES  12 C  268  THR PRO GLU GLU ALA ILE ARG GLU GLY TRP LYS LEU SER
SEQRES  13 C  268  PHE SER GLU GLU PHE ILE HIS THR HIS LYS ALA GLU LEU
SEQRES  14 C  268  GLU ALA HIS ILE PRO ARG LEU LEU ALA GLN LEU THR PRO
SEQRES  15 C  268  ARG PHE ALA TYR GLU ARG HIS PHE GLN ALA THR MET THR
SEQRES  16 C  268  LEU ARG VAL PHE LYS GLN LEU LYS GLU ILE GLN ALA PRO
SEQRES  17 C  268  THR LEU VAL ALA THR GLY ARG ASP ASP MET LEU ILE PRO
SEQRES  18 C  268  ALA VAL ASN SER GLU ILE LEU ALA ARG GLU ILE PRO GLY
SEQRES  19 C  268  ALA GLU LEU ALA ILE PHE GLU SER ALA GLY HIS GLY PHE
SEQRES  20 C  268  VAL THR SER ALA ARG GLU PRO PHE LEU LYS VAL LEU LYS
SEQRES  21 C  268  GLU PHE LEU ALA ARG GLN SER VAL
SEQRES   1 D  268  MET LEU TYR ALA GLN VAL ASN GLY ILE ASN LEU HIS TYR
SEQRES   2 D  268  GLU ILE GLU GLY GLN GLY GLN PRO LEU LEU LEU ILE MET
SEQRES   3 D  268  GLY LEU GLY ALA PRO ALA ALA ALA TRP ASP PRO ILE PHE
SEQRES   4 D  268  VAL GLN THR LEU THR LYS THR HIS GLN VAL ILE ILE TYR
SEQRES   5 D  268  ASP ASN ARG GLY THR GLY LEU SER ASP LYS PRO ASP MET
SEQRES   6 D  268  PRO TYR SER ILE ALA MET PHE ALA SER ASP ALA VAL GLY
SEQRES   7 D  268  LEU LEU ASP ALA LEU ASN ILE PRO ARG ALA HIS VAL PHE
SEQRES   8 D  268  GLY VAL SER MET GLY GLY MET ILE ALA GLN GLU LEU ALA
SEQRES   9 D  268  ILE HIS TYR PRO GLN ARG VAL ALA SER LEU ILE LEU GLY
SEQRES  10 D  268  CYS THR THR PRO GLY GLY LYS HIS ALA VAL PRO ALA PRO
SEQRES  11 D  268  PRO GLU SER LEU LYS ALA LEU GLU GLY ARG ALA GLY LEU
SEQRES  12 D  268  THR PRO GLU GLU ALA ILE ARG GLU GLY TRP LYS LEU SER
SEQRES  13 D  268  PHE SER GLU GLU PHE ILE HIS THR HIS LYS ALA GLU LEU
SEQRES  14 D  268  GLU ALA HIS ILE PRO ARG LEU LEU ALA GLN LEU THR PRO
SEQRES  15 D  268  ARG PHE ALA TYR GLU ARG HIS PHE GLN ALA THR MET THR
SEQRES  16 D  268  LEU ARG VAL PHE LYS GLN LEU LYS GLU ILE GLN ALA PRO
SEQRES  17 D  268  THR LEU VAL ALA THR GLY ARG ASP ASP MET LEU ILE PRO
SEQRES  18 D  268  ALA VAL ASN SER GLU ILE LEU ALA ARG GLU ILE PRO GLY
SEQRES  19 D  268  ALA GLU LEU ALA ILE PHE GLU SER ALA GLY HIS GLY PHE
SEQRES  20 D  268  VAL THR SER ALA ARG GLU PRO PHE LEU LYS VAL LEU LYS
SEQRES  21 D  268  GLU PHE LEU ALA ARG GLN SER VAL
HET    GOL  A 301       6
HET    GOL  A 302       6
HET    GOL  A 303       6
HET    J3J  A 304      11
HET    EDO  B 301       4
HET    J3G  B 302      16
HET    PO4  B 303       5
HET    ABU  C 301       6
HET    SO4  C 302       5
HET    SO4  C 303       5
HET    EDO  C 304       4
HET    EDO  C 305       4
HET    ABU  D 301       6
HET    SO4  D 302       5
HET    SO4  D 303       5
HET    GOL  D 304       6
HETNAM     GOL GLYCEROL
HETNAM     J3J 1,3-DIHYDROXYPROPAN-2-YL BUTANOATE
HETNAM     EDO 1,2-ETHANEDIOL
HETNAM     J3G 2-HYDROXYPROPANE-1,3-DIYL DIBUTANOATE
HETNAM     PO4 PHOSPHATE ION
HETNAM     ABU GAMMA-AMINO-BUTANOIC ACID
HETNAM     SO4 SULFATE ION
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL
HETSYN     EDO ETHYLENE GLYCOL
HETSYN     ABU GAMMA(AMINO)-BUTYRIC ACID
FORMUL   5  GOL    4(C3 H8 O3)
FORMUL   8  J3J    C7 H14 O4
FORMUL   9  EDO    3(C2 H6 O2)
FORMUL  10  J3G    C11 H20 O5
FORMUL  11  PO4    O4 P 3-
FORMUL  12  ABU    2(C4 H9 N O2)
FORMUL  13  SO4    4(O4 S 2-)
FORMUL  21  HOH   *391(H2 O)
HELIX    1 AA1 PRO A   31  TRP A   35  5                                   5
HELIX    2 AA2 ASP A   36  LYS A   45  1                                  10
HELIX    3 AA3 SER A   68  LEU A   83  1                                  16
HELIX    4 AA4 SER A   94  TYR A  107  1                                  14
HELIX    5 AA5 THR A  144  PHE A  157  1                                  14
HELIX    6 AA6 SER A  158  HIS A  165  1                                   8
HELIX    7 AA7 HIS A  165  HIS A  172  1                                   8
HELIX    8 AA8 HIS A  172  ALA A  178  1                                   7
HELIX    9 AA9 PRO A  182  THR A  193  1                                  12
HELIX   10 AB1 MET A  194  LEU A  196  5                                   3
HELIX   11 AB2 VAL A  198  ILE A  205  5                                   8
HELIX   12 AB3 ALA A  222  ILE A  232  1                                  11
HELIX   13 AB4 GLY A  246  ALA A  251  1                                   6
HELIX   14 AB5 ALA A  251  ARG A  265  1                                  15
HELIX   15 AB6 PRO B   31  TRP B   35  5                                   5
HELIX   16 AB7 ASP B   36  LYS B   45  1                                  10
HELIX   17 AB8 SER B   68  LEU B   83  1                                  16
HELIX   18 AB9 SER B   94  TYR B  107  1                                  14
HELIX   19 AC1 PRO B  130  ARG B  140  1                                  11
HELIX   20 AC2 ALA B  141  LEU B  143  5                                   3
HELIX   21 AC3 THR B  144  PHE B  157  1                                  14
HELIX   22 AC4 SER B  158  HIS B  165  1                                   8
HELIX   23 AC5 HIS B  165  HIS B  172  1                                   8
HELIX   24 AC6 HIS B  172  ALA B  178  1                                   7
HELIX   25 AC7 PRO B  182  THR B  193  1                                  12
HELIX   26 AC8 MET B  194  LEU B  196  5                                   3
HELIX   27 AC9 VAL B  198  ILE B  205  5                                   8
HELIX   28 AD1 ALA B  222  ILE B  232  1                                  11
HELIX   29 AD2 GLY B  246  ALA B  251  1                                   6
HELIX   30 AD3 ALA B  251  ARG B  265  1                                  15
HELIX   31 AD4 PRO C   31  TRP C   35  5                                   5
HELIX   32 AD5 ASP C   36  LYS C   45  1                                  10
HELIX   33 AD6 SER C   68  LEU C   83  1                                  16
HELIX   34 AD7 SER C   94  TYR C  107  1                                  14
HELIX   35 AD8 PRO C  130  ALA C  136  1                                   7
HELIX   36 AD9 PRO C  145  PHE C  157  1                                  13
HELIX   37 AE1 SER C  158  HIS C  165  1                                   8
HELIX   38 AE2 HIS C  165  HIS C  172  1                                   8
HELIX   39 AE3 HIS C  172  ALA C  178  1                                   7
HELIX   40 AE4 PRO C  182  THR C  193  1                                  12
HELIX   41 AE5 VAL C  198  ILE C  205  5                                   8
HELIX   42 AE6 ALA C  222  ILE C  232  1                                  11
HELIX   43 AE7 GLY C  246  ALA C  251  1                                   6
HELIX   44 AE8 ALA C  251  ARG C  265  1                                  15
HELIX   45 AE9 PRO D   31  TRP D   35  5                                   5
HELIX   46 AF1 ASP D   36  LYS D   45  1                                  10
HELIX   47 AF2 SER D   68  LEU D   83  1                                  16
HELIX   48 AF3 SER D   94  TYR D  107  1                                  14
HELIX   49 AF4 PRO D  130  ARG D  140  1                                  11
HELIX   50 AF5 ALA D  141  LEU D  143  5                                   3
HELIX   51 AF6 THR D  144  PHE D  157  1                                  14
HELIX   52 AF7 SER D  158  HIS D  165  1                                   8
HELIX   53 AF8 HIS D  165  HIS D  172  1                                   8
HELIX   54 AF9 HIS D  172  ALA D  178  1                                   7
HELIX   55 AG1 PRO D  182  THR D  193  1                                  12
HELIX   56 AG2 VAL D  198  ILE D  205  5                                   8
HELIX   57 AG3 ALA D  222  ILE D  232  1                                  11
HELIX   58 AG4 GLY D  246  ALA D  251  1                                   6
HELIX   59 AG5 ALA D  251  ARG D  265  1                                  15
SHEET    1 AA1 8 TYR A   3  VAL A   6  0
SHEET    2 AA1 8 ILE A   9  GLU A  16 -1  O  ILE A   9   N  VAL A   6
SHEET    3 AA1 8 HIS A  47  TYR A  52 -1  O  VAL A  49   N  GLU A  16
SHEET    4 AA1 8 GLN A  20  ILE A  25  1  N  LEU A  22   O  ILE A  50
SHEET    5 AA1 8 ALA A  88  VAL A  93  1  O  HIS A  89   N  LEU A  23
SHEET    6 AA1 8 VAL A 111  GLY A 117  1  O  GLY A 117   N  GLY A  92
SHEET    7 AA1 8 THR A 209  GLY A 214  1  O  ALA A 212   N  LEU A 116
SHEET    8 AA1 8 GLU A 236  PHE A 240  1  O  GLU A 236   N  VAL A 211
SHEET    1 AA2 8 LEU B   2  VAL B   6  0
SHEET    2 AA2 8 ILE B   9  GLU B  16 -1  O  ILE B   9   N  VAL B   6
SHEET    3 AA2 8 HIS B  47  TYR B  52 -1  O  VAL B  49   N  GLU B  16
SHEET    4 AA2 8 GLN B  20  ILE B  25  1  N  LEU B  22   O  ILE B  50
SHEET    5 AA2 8 ALA B  88  VAL B  93  1  O  HIS B  89   N  LEU B  23
SHEET    6 AA2 8 VAL B 111  GLY B 117  1  O  GLY B 117   N  GLY B  92
SHEET    7 AA2 8 THR B 209  GLY B 214  1  O  ALA B 212   N  LEU B 116
SHEET    8 AA2 8 GLU B 236  PHE B 240  1  O  GLU B 236   N  VAL B 211
SHEET    1 AA3 8 LEU C   2  VAL C   6  0
SHEET    2 AA3 8 ILE C   9  GLU C  16 -1  O  ILE C   9   N  VAL C   6
SHEET    3 AA3 8 HIS C  47  TYR C  52 -1  O  VAL C  49   N  GLU C  16
SHEET    4 AA3 8 GLN C  20  ILE C  25  1  N  LEU C  22   O  ILE C  50
SHEET    5 AA3 8 ALA C  88  VAL C  93  1  O  HIS C  89   N  LEU C  23
SHEET    6 AA3 8 VAL C 111  GLY C 117  1  O  GLY C 117   N  GLY C  92
SHEET    7 AA3 8 THR C 209  GLY C 214  1  O  ALA C 212   N  LEU C 116
SHEET    8 AA3 8 GLU C 236  PHE C 240  1  O  GLU C 236   N  VAL C 211
SHEET    1 AA4 8 TYR D   3  VAL D   6  0
SHEET    2 AA4 8 ILE D   9  GLU D  16 -1  O  ILE D   9   N  VAL D   6
SHEET    3 AA4 8 HIS D  47  TYR D  52 -1  O  VAL D  49   N  GLU D  16
SHEET    4 AA4 8 GLN D  20  ILE D  25  1  N  LEU D  22   O  ILE D  50
SHEET    5 AA4 8 ALA D  88  VAL D  93  1  O  HIS D  89   N  LEU D  23
SHEET    6 AA4 8 VAL D 111  GLY D 117  1  O  GLY D 117   N  GLY D  92
SHEET    7 AA4 8 THR D 209  GLY D 214  1  O  ALA D 212   N  LEU D 116
SHEET    8 AA4 8 GLU D 236  PHE D 240  1  O  GLU D 236   N  VAL D 211
SITE     1 AC1  7 LEU A  28  SER A  94  MET A  95  LEU A 134
SITE     2 AC1  7 LEU A 219  ILE A 220  HOH A 449
SITE     1 AC2  4 GLU A  14  GLU A  16  HOH A 421  HOH D 445
SITE     1 AC3  5 SER A 133  MET A 218  HOH A 443  ILE C 239
SITE     2 AC3  5 VAL C 258
SITE     1 AC4  5 ILE A  15  GLY A  17  GLN A  48  HOH A 402
SITE     2 AC4  5 HOH A 425
SITE     1 AC5  1 HIS B 125
SITE     1 AC6 10 SER B  68  ILE B  69  ALA B  70  GLU B 102
SITE     2 AC6 10 HIS B 106  THR B 195  ARG B 197  GLN B 201
SITE     3 AC6 10 HOH B 410  HOH B 483
SITE     1 AC7  2 GLN B  48  ILE D  15
SITE     1 AC8  3 LEU C  28  SER C  94  MET C  95
SITE     1 AC9  2 ARG C 265  GLN C 266
SITE     1 AD1  3 TYR C 107  EDO C 304  HOH C 417
SITE     1 AD2  2 HIS C 106  SO4 C 303
SITE     1 AD3  2 HIS C 172  THR C 249
SITE     1 AD4  5 GLY D  27  LEU D  28  SER D  94  MET D  95
SITE     2 AD4  5 HIS D 245
SITE     1 AD5  3 GLY D 123  LYS D 124  ARG D 197
SITE     1 AD6  3 LYS D  62  HOH D 457  HOH D 461
SITE     1 AD7  6 GLY D 122  PRO D 128  ALA D 129  THR D 193
SITE     2 AD7  6 HOH D 410  HOH D 427
CRYST1   71.650  105.950  145.940  90.00  90.00  90.00 P 21 21 21   16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.013957  0.000000  0.000000        0.00000
SCALE2      0.000000  0.009438  0.000000        0.00000
SCALE3      0.000000  0.000000  0.006852        0.00000
TER    2061      VAL A 268
TER    4174      VAL B 268
TER    6221      VAL C 268
TER    8304      VAL D 268
MASTER      505    0   16   59   32    0   23    6 8680    4  100   84
END