longtext: 6kf7-pdb

content
HEADER    HYDROLASE                               06-JUL-19   6KF7
TITLE     MICROBIAL HORMONE-SENSITIVE LIPASE E53 MUTANT S285G
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: LIPASE;
COMPND   3 CHAIN: A, B, C, D;
COMPND   4 ENGINEERED: YES;
COMPND   5 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: ERYTHROBACTER LONGUS;
SOURCE   3 ORGANISM_TAXID: 1044;
SOURCE   4 GENE: EH31_02760;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI 'BL21-GOLD(DE3)PLYSS AG';
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 866768
KEYWDS    ESTERASE, MICROBIAL HORMONE-SENSITIVE LIPASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    Y.XIAOCHEN,L.ZHENGYANG,X.XUEWEI,L.JIXI
REVDAT   1   08-JUL-20 6KF7    0
JRNL        AUTH   Y.XIAOCHEN
JRNL        TITL   MICROBIAL HORMONE-SENSITIVE LIPASE E53 MUTANT S285G
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.80 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.80
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 48.53
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.360
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.6
REMARK   3   NUMBER OF REFLECTIONS             : 186898
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.170
REMARK   3   R VALUE            (WORKING SET) : 0.169
REMARK   3   FREE R VALUE                     : 0.191
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.030
REMARK   3   FREE R VALUE TEST SET COUNT      : 9409
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 48.5300 -  5.5800    0.99     6329   315  0.1722 0.1777
REMARK   3     2  5.5800 -  4.4300    1.00     6122   284  0.1454 0.1570
REMARK   3     3  4.4300 -  3.8700    1.00     6045   342  0.1341 0.1456
REMARK   3     4  3.8700 -  3.5200    1.00     5991   325  0.1444 0.1627
REMARK   3     5  3.5200 -  3.2700    1.00     6012   301  0.1519 0.1751
REMARK   3     6  3.2700 -  3.0700    1.00     5936   315  0.1574 0.1875
REMARK   3     7  3.0700 -  2.9200    1.00     5994   321  0.1669 0.1861
REMARK   3     8  2.9200 -  2.7900    1.00     5946   328  0.1673 0.2008
REMARK   3     9  2.7900 -  2.6900    1.00     5929   323  0.1664 0.2047
REMARK   3    10  2.6900 -  2.5900    1.00     5878   337  0.1691 0.1960
REMARK   3    11  2.5900 -  2.5100    1.00     5906   355  0.1729 0.2036
REMARK   3    12  2.5100 -  2.4400    1.00     5941   294  0.1753 0.2137
REMARK   3    13  2.4400 -  2.3800    1.00     5935   298  0.1755 0.1964
REMARK   3    14  2.3800 -  2.3200    1.00     5910   305  0.1775 0.2029
REMARK   3    15  2.3200 -  2.2700    1.00     5921   310  0.1726 0.2003
REMARK   3    16  2.2700 -  2.2200    1.00     5866   335  0.1747 0.2030
REMARK   3    17  2.2200 -  2.1700    1.00     5902   325  0.1809 0.2119
REMARK   3    18  2.1700 -  2.1300    1.00     5857   321  0.1826 0.2065
REMARK   3    19  2.1300 -  2.0900    1.00     5904   302  0.1918 0.2338
REMARK   3    20  2.0900 -  2.0600    1.00     5843   333  0.1896 0.2175
REMARK   3    21  2.0600 -  2.0200    1.00     5873   287  0.1873 0.2167
REMARK   3    22  2.0200 -  1.9900    1.00     5874   308  0.1908 0.2188
REMARK   3    23  1.9900 -  1.9600    1.00     5859   310  0.2045 0.2423
REMARK   3    24  1.9600 -  1.9400    0.99     5869   315  0.2136 0.2531
REMARK   3    25  1.9400 -  1.9100    1.00     5855   293  0.2256 0.2405
REMARK   3    26  1.9100 -  1.8900    0.99     5905   289  0.2208 0.2482
REMARK   3    27  1.8900 -  1.8600    1.00     5798   322  0.2229 0.2328
REMARK   3    28  1.8600 -  1.8400    0.99     5880   313  0.2358 0.2603
REMARK   3    29  1.8400 -  1.8200    1.00     5826   306  0.2489 0.2707
REMARK   3    30  1.8200 -  1.8000    0.95     5583   297  0.2669 0.2817
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : NULL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.175
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 18.941
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 24.48
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 28.49
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.007           9545
REMARK   3   ANGLE     :  0.833          12956
REMARK   3   CHIRALITY :  0.052           1471
REMARK   3   PLANARITY :  0.006           1708
REMARK   3   DIHEDRAL  :  8.309           5709
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 6KF7 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 12-JUL-19.
REMARK 100 THE DEPOSITION ID IS D_1300012868.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 01-MAY-17
REMARK 200  TEMPERATURE           (KELVIN) : 80
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRF
REMARK 200  BEAMLINE                       : BL19U1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97916
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 186934
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.800
REMARK 200  RESOLUTION RANGE LOW       (A) : 48.530
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.6
REMARK 200  DATA REDUNDANCY                : 6.500
REMARK 200  R MERGE                    (I) : 0.11900
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 21.7560
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.80
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.86
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 0.54800
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: 4YPV
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 67.62
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.80
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: CALCIUM CHLORIDE, BIS-TRIS, PEG MME
REMARK 280  550, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 2 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y,-Z
REMARK 290       3555   X+1/2,-Y,-Z+1/2
REMARK 290       4555   -X+1/2,-Y,Z+1/2
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       35.32850
REMARK 290   SMTRY2   3  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000      109.73200
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       35.32850
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000      109.73200
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH B 782  LIES ON A SPECIAL POSITION.
REMARK 375      HOH B 794  LIES ON A SPECIAL POSITION.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     THR A     2
REMARK 465     ASP A     3
REMARK 465     THR A   313
REMARK 465     ALA A   314
REMARK 465     MET B     1
REMARK 465     THR B     2
REMARK 465     ASP B     3
REMARK 465     THR B   313
REMARK 465     ALA B   314
REMARK 465     MET C     1
REMARK 465     THR C     2
REMARK 465     ASP C     3
REMARK 465     THR C   313
REMARK 465     ALA C   314
REMARK 465     MET D     1
REMARK 465     THR D     2
REMARK 465     ASP D     3
REMARK 465     THR D   313
REMARK 465     ALA D   314
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     THR A   4    OG1  CG2
REMARK 470     GLU A  51    CG   CD   OE1  OE2
REMARK 470     GLU A  76    CG   CD   OE1  OE2
REMARK 470     GLU A  79    CG   CD   OE1  OE2
REMARK 470     LYS A 178    CG   CD   CE   NZ
REMARK 470     THR B   4    OG1  CG2
REMARK 470     LYS B 178    CG   CD   CE   NZ
REMARK 470     PRO B 294    CB   CG
REMARK 470     THR C   4    OG1  CG2
REMARK 470     GLU C  79    CG   CD   OE1  OE2
REMARK 470     LYS C 178    CG   CD   CE   NZ
REMARK 470     THR D   4    OG1  CG2
REMARK 470     LYS D 178    CG   CD   CE   NZ
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OG   SER A   162     CAL  D8F A   401              1.78
REMARK 500   OG   SER C   162     CAL  D8F C   401              1.85
REMARK 500   OG   SER B   162     CAL  D8F B   401              1.86
REMARK 500   OG   SER D   162     CAL  D8F D   401              1.92
REMARK 500   OG   SER B   162     CAQ  D8F B   401              1.96
REMARK 500   O1   EDO B   402     O    HOH B   501              2.00
REMARK 500   OG   SER C   162     CAQ  D8F C   401              2.01
REMARK 500   OAB  D8F A   401     O    HOH A   501              2.05
REMARK 500   OG   SER D   162     CAQ  D8F D   401              2.05
REMARK 500   OG   SER A   162     CAQ  D8F A   401              2.05
REMARK 500   O    HOH B   669     O    HOH B   788              2.06
REMARK 500   O    HOH C   746     O    HOH C   864              2.06
REMARK 500   O    HOH D   635     O    HOH D   736              2.10
REMARK 500   O    HOH B   718     O    HOH B   774              2.10
REMARK 500   O    HOH C   608     O    HOH C   746              2.13
REMARK 500   O    HOH A   780     O    HOH A   785              2.14
REMARK 500   O    HOH A   503     O    HOH A   580              2.17
REMARK 500   O    HOH D   651     O    HOH D   756              2.17
REMARK 500   O    HOH A   758     O    HOH B   649              2.17
REMARK 500   O    HOH D   756     O    HOH D   795              2.18
REMARK 500   O    HOH C   679     O    HOH C   824              2.18
REMARK 500   O    HOH A   581     O    HOH A   782              2.18
REMARK 500   O    HOH B   722     O    HOH B   816              2.18
REMARK 500   O    HOH D   756     O    HOH D   779              2.18
REMARK 500   O    HOH C   738     O    HOH C   815              2.19
REMARK 500   O    HOH A   655     O    HOH B   579              2.19
REMARK 500   O    HOH D   687     O    HOH D   739              2.19
REMARK 500   O    HOH A   783     O    HOH A   820              2.19
REMARK 500   O    HOH C   578     O    HOH C   781              2.19
REMARK 500   O    HOH A   543     O    HOH A   809              2.19
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   O    HOH D   501     O    HOH D   501     2754     1.78
REMARK 500   O    HOH D   716     O    HOH D   716     2754     1.79
REMARK 500   O    HOH B   753     O    HOH B   753     2555     2.04
REMARK 500   O    HOH B   742     O    HOH B   753     2555     2.07
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASP A  96     -160.64   -164.49
REMARK 500    PRO A 129       33.65    -99.69
REMARK 500    SER A 162     -119.05     65.06
REMARK 500    PHE A 191       64.69     24.62
REMARK 500    VAL A 211      -71.61     73.95
REMARK 500    ASP B  96     -159.44   -165.09
REMARK 500    SER B 162     -119.55     62.09
REMARK 500    LEU B 186      141.06   -171.76
REMARK 500    PHE B 191       65.95     22.58
REMARK 500    VAL B 211      -69.64     68.10
REMARK 500    ALA C  22     -132.24     55.86
REMARK 500    ASP C  96     -161.14   -163.53
REMARK 500    SER C 162     -119.38     64.92
REMARK 500    LEU C 186      139.25   -173.20
REMARK 500    PHE C 191       63.58     23.89
REMARK 500    VAL C 211      -71.24     71.21
REMARK 500    ASP D  96     -160.10   -164.72
REMARK 500    SER D 162     -118.64     61.69
REMARK 500    PHE D 191       64.84     22.44
REMARK 500    VAL D 211      -68.29     67.01
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A 867        DISTANCE =  5.93 ANGSTROMS
REMARK 525    HOH A 868        DISTANCE =  6.14 ANGSTROMS
REMARK 525    HOH C 887        DISTANCE =  5.81 ANGSTROMS
REMARK 525    HOH C 888        DISTANCE =  5.92 ANGSTROMS
REMARK 525    HOH C 889        DISTANCE =  6.00 ANGSTROMS
REMARK 525    HOH C 890        DISTANCE =  6.19 ANGSTROMS
REMARK 525    HOH D 828        DISTANCE =  6.35 ANGSTROMS
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue D8F A 401
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 402
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 403
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 404
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 405
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 406
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 407
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 408
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 409
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 410
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 411
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 412
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue 6NA A 413
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 402
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 403
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL B 404
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL B 405
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL B 406
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 402
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 403
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 404
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL C 405
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL C 406
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL C 407
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL C 408
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue 6NA C 409
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PGE C 410
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 D 402
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 403
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 404
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 405
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL D 406
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL D 407
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL D 408
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL D 409
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue NPO D 410
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for Di-peptide D8F B 401 and SER B
REMARK 800  162
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for Di-peptide D8F C 401 and SER C
REMARK 800  162
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for Di-peptide D8F D 401 and SER D
REMARK 800  162
DBREF1 6KF7 A    1   314  UNP                  A0A074MDU6_ERYLO
DBREF2 6KF7 A     A0A074MDU6                          1         314
DBREF1 6KF7 B    1   314  UNP                  A0A074MDU6_ERYLO
DBREF2 6KF7 B     A0A074MDU6                          1         314
DBREF1 6KF7 C    1   314  UNP                  A0A074MDU6_ERYLO
DBREF2 6KF7 C     A0A074MDU6                          1         314
DBREF1 6KF7 D    1   314  UNP                  A0A074MDU6_ERYLO
DBREF2 6KF7 D     A0A074MDU6                          1         314
SEQADV 6KF7 GLY A  285  UNP  A0A074MDU SER   285 ENGINEERED MUTATION
SEQADV 6KF7 GLY B  285  UNP  A0A074MDU SER   285 ENGINEERED MUTATION
SEQADV 6KF7 GLY C  285  UNP  A0A074MDU SER   285 ENGINEERED MUTATION
SEQADV 6KF7 GLY D  285  UNP  A0A074MDU SER   285 ENGINEERED MUTATION
SEQRES   1 A  314  MET THR ASP THR PRO PHE ILE ARG PRO ASP MET LYS ALA
SEQRES   2 A  314  PHE LEU GLU ALA ILE ALA ALA MET ALA GLY PRO THR LEU
SEQRES   3 A  314  ALA GLU MET THR LEU GLU GLU ALA ARG ALA SER TYR VAL
SEQRES   4 A  314  ALA LEU HIS GLY MET ALA ASP ARG PRO ALA ARG GLU LEU
SEQRES   5 A  314  ALA VAL ILE ARG ASN LEU SER CYS PRO GLY PRO ALA GLY
SEQRES   6 A  314  ASP ILE PRO LEU ARG LEU TYR ASP ALA ARG GLU SER ARG
SEQRES   7 A  314  GLU ALA GLY PRO VAL ILE THR PHE TYR HIS GLY GLY GLY
SEQRES   8 A  314  PHE VAL ILE GLY ASP LEU ASP THR HIS HIS ASN LEU CYS
SEQRES   9 A  314  THR GLU ILE ALA ALA LEU MET ASP LEU PRO VAL VAL ALA
SEQRES  10 A  314  VAL ASP TYR ARG LEU ALA PRO GLU HIS PRO PHE PRO ALA
SEQRES  11 A  314  ALA ILE GLU ASP CYS GLU ALA ALA THR ARG TRP VAL ALA
SEQRES  12 A  314  SER SER PRO SER GLU LEU GLY ARG THR ALA SER GLY VAL
SEQRES  13 A  314  ILE PRO ILE GLY ASP SER ALA GLY GLY ASN ALA THR ILE
SEQRES  14 A  314  VAL VAL SER GLN LEU LEU GLY ALA LYS PRO ALA ASP VAL
SEQRES  15 A  314  PRO VAL VAL LEU GLN VAL PRO ILE PHE PRO LEU ALA SER
SEQRES  16 A  314  ASP ALA VAL GLY SER ALA SER LEU GLU ALA PHE ALA GLU
SEQRES  17 A  314  GLY PHE VAL LEU THR LYS ALA SER ILE GLU PHE PHE ASP
SEQRES  18 A  314  THR ALA TYR LYS ALA ASP ARG ALA ASP PRO ARG GLY PHE
SEQRES  19 A  314  PRO ILE LEU GLY ASP HIS THR ALA ALA PRO PRO THR ILE
SEQRES  20 A  314  VAL ALA THR ALA SER LEU ASP PRO ILE ARG ASP SER GLY
SEQRES  21 A  314  ARG ASP TYR ALA LYS ALA LEU VAL GLU ALA GLY ARG ASP
SEQRES  22 A  314  VAL VAL TYR LEU GLU MET GLU GLY VAL THR HIS GLY PHE
SEQRES  23 A  314  THR ASN ILE ARG ALA ALA VAL PRO SER THR GLN GLY ASP
SEQRES  24 A  314  LEU GLU ARG ILE ILE ALA ALA MET LYS MET MET LEU GLY
SEQRES  25 A  314  THR ALA
SEQRES   1 B  314  MET THR ASP THR PRO PHE ILE ARG PRO ASP MET LYS ALA
SEQRES   2 B  314  PHE LEU GLU ALA ILE ALA ALA MET ALA GLY PRO THR LEU
SEQRES   3 B  314  ALA GLU MET THR LEU GLU GLU ALA ARG ALA SER TYR VAL
SEQRES   4 B  314  ALA LEU HIS GLY MET ALA ASP ARG PRO ALA ARG GLU LEU
SEQRES   5 B  314  ALA VAL ILE ARG ASN LEU SER CYS PRO GLY PRO ALA GLY
SEQRES   6 B  314  ASP ILE PRO LEU ARG LEU TYR ASP ALA ARG GLU SER ARG
SEQRES   7 B  314  GLU ALA GLY PRO VAL ILE THR PHE TYR HIS GLY GLY GLY
SEQRES   8 B  314  PHE VAL ILE GLY ASP LEU ASP THR HIS HIS ASN LEU CYS
SEQRES   9 B  314  THR GLU ILE ALA ALA LEU MET ASP LEU PRO VAL VAL ALA
SEQRES  10 B  314  VAL ASP TYR ARG LEU ALA PRO GLU HIS PRO PHE PRO ALA
SEQRES  11 B  314  ALA ILE GLU ASP CYS GLU ALA ALA THR ARG TRP VAL ALA
SEQRES  12 B  314  SER SER PRO SER GLU LEU GLY ARG THR ALA SER GLY VAL
SEQRES  13 B  314  ILE PRO ILE GLY ASP SER ALA GLY GLY ASN ALA THR ILE
SEQRES  14 B  314  VAL VAL SER GLN LEU LEU GLY ALA LYS PRO ALA ASP VAL
SEQRES  15 B  314  PRO VAL VAL LEU GLN VAL PRO ILE PHE PRO LEU ALA SER
SEQRES  16 B  314  ASP ALA VAL GLY SER ALA SER LEU GLU ALA PHE ALA GLU
SEQRES  17 B  314  GLY PHE VAL LEU THR LYS ALA SER ILE GLU PHE PHE ASP
SEQRES  18 B  314  THR ALA TYR LYS ALA ASP ARG ALA ASP PRO ARG GLY PHE
SEQRES  19 B  314  PRO ILE LEU GLY ASP HIS THR ALA ALA PRO PRO THR ILE
SEQRES  20 B  314  VAL ALA THR ALA SER LEU ASP PRO ILE ARG ASP SER GLY
SEQRES  21 B  314  ARG ASP TYR ALA LYS ALA LEU VAL GLU ALA GLY ARG ASP
SEQRES  22 B  314  VAL VAL TYR LEU GLU MET GLU GLY VAL THR HIS GLY PHE
SEQRES  23 B  314  THR ASN ILE ARG ALA ALA VAL PRO SER THR GLN GLY ASP
SEQRES  24 B  314  LEU GLU ARG ILE ILE ALA ALA MET LYS MET MET LEU GLY
SEQRES  25 B  314  THR ALA
SEQRES   1 C  314  MET THR ASP THR PRO PHE ILE ARG PRO ASP MET LYS ALA
SEQRES   2 C  314  PHE LEU GLU ALA ILE ALA ALA MET ALA GLY PRO THR LEU
SEQRES   3 C  314  ALA GLU MET THR LEU GLU GLU ALA ARG ALA SER TYR VAL
SEQRES   4 C  314  ALA LEU HIS GLY MET ALA ASP ARG PRO ALA ARG GLU LEU
SEQRES   5 C  314  ALA VAL ILE ARG ASN LEU SER CYS PRO GLY PRO ALA GLY
SEQRES   6 C  314  ASP ILE PRO LEU ARG LEU TYR ASP ALA ARG GLU SER ARG
SEQRES   7 C  314  GLU ALA GLY PRO VAL ILE THR PHE TYR HIS GLY GLY GLY
SEQRES   8 C  314  PHE VAL ILE GLY ASP LEU ASP THR HIS HIS ASN LEU CYS
SEQRES   9 C  314  THR GLU ILE ALA ALA LEU MET ASP LEU PRO VAL VAL ALA
SEQRES  10 C  314  VAL ASP TYR ARG LEU ALA PRO GLU HIS PRO PHE PRO ALA
SEQRES  11 C  314  ALA ILE GLU ASP CYS GLU ALA ALA THR ARG TRP VAL ALA
SEQRES  12 C  314  SER SER PRO SER GLU LEU GLY ARG THR ALA SER GLY VAL
SEQRES  13 C  314  ILE PRO ILE GLY ASP SER ALA GLY GLY ASN ALA THR ILE
SEQRES  14 C  314  VAL VAL SER GLN LEU LEU GLY ALA LYS PRO ALA ASP VAL
SEQRES  15 C  314  PRO VAL VAL LEU GLN VAL PRO ILE PHE PRO LEU ALA SER
SEQRES  16 C  314  ASP ALA VAL GLY SER ALA SER LEU GLU ALA PHE ALA GLU
SEQRES  17 C  314  GLY PHE VAL LEU THR LYS ALA SER ILE GLU PHE PHE ASP
SEQRES  18 C  314  THR ALA TYR LYS ALA ASP ARG ALA ASP PRO ARG GLY PHE
SEQRES  19 C  314  PRO ILE LEU GLY ASP HIS THR ALA ALA PRO PRO THR ILE
SEQRES  20 C  314  VAL ALA THR ALA SER LEU ASP PRO ILE ARG ASP SER GLY
SEQRES  21 C  314  ARG ASP TYR ALA LYS ALA LEU VAL GLU ALA GLY ARG ASP
SEQRES  22 C  314  VAL VAL TYR LEU GLU MET GLU GLY VAL THR HIS GLY PHE
SEQRES  23 C  314  THR ASN ILE ARG ALA ALA VAL PRO SER THR GLN GLY ASP
SEQRES  24 C  314  LEU GLU ARG ILE ILE ALA ALA MET LYS MET MET LEU GLY
SEQRES  25 C  314  THR ALA
SEQRES   1 D  314  MET THR ASP THR PRO PHE ILE ARG PRO ASP MET LYS ALA
SEQRES   2 D  314  PHE LEU GLU ALA ILE ALA ALA MET ALA GLY PRO THR LEU
SEQRES   3 D  314  ALA GLU MET THR LEU GLU GLU ALA ARG ALA SER TYR VAL
SEQRES   4 D  314  ALA LEU HIS GLY MET ALA ASP ARG PRO ALA ARG GLU LEU
SEQRES   5 D  314  ALA VAL ILE ARG ASN LEU SER CYS PRO GLY PRO ALA GLY
SEQRES   6 D  314  ASP ILE PRO LEU ARG LEU TYR ASP ALA ARG GLU SER ARG
SEQRES   7 D  314  GLU ALA GLY PRO VAL ILE THR PHE TYR HIS GLY GLY GLY
SEQRES   8 D  314  PHE VAL ILE GLY ASP LEU ASP THR HIS HIS ASN LEU CYS
SEQRES   9 D  314  THR GLU ILE ALA ALA LEU MET ASP LEU PRO VAL VAL ALA
SEQRES  10 D  314  VAL ASP TYR ARG LEU ALA PRO GLU HIS PRO PHE PRO ALA
SEQRES  11 D  314  ALA ILE GLU ASP CYS GLU ALA ALA THR ARG TRP VAL ALA
SEQRES  12 D  314  SER SER PRO SER GLU LEU GLY ARG THR ALA SER GLY VAL
SEQRES  13 D  314  ILE PRO ILE GLY ASP SER ALA GLY GLY ASN ALA THR ILE
SEQRES  14 D  314  VAL VAL SER GLN LEU LEU GLY ALA LYS PRO ALA ASP VAL
SEQRES  15 D  314  PRO VAL VAL LEU GLN VAL PRO ILE PHE PRO LEU ALA SER
SEQRES  16 D  314  ASP ALA VAL GLY SER ALA SER LEU GLU ALA PHE ALA GLU
SEQRES  17 D  314  GLY PHE VAL LEU THR LYS ALA SER ILE GLU PHE PHE ASP
SEQRES  18 D  314  THR ALA TYR LYS ALA ASP ARG ALA ASP PRO ARG GLY PHE
SEQRES  19 D  314  PRO ILE LEU GLY ASP HIS THR ALA ALA PRO PRO THR ILE
SEQRES  20 D  314  VAL ALA THR ALA SER LEU ASP PRO ILE ARG ASP SER GLY
SEQRES  21 D  314  ARG ASP TYR ALA LYS ALA LEU VAL GLU ALA GLY ARG ASP
SEQRES  22 D  314  VAL VAL TYR LEU GLU MET GLU GLY VAL THR HIS GLY PHE
SEQRES  23 D  314  THR ASN ILE ARG ALA ALA VAL PRO SER THR GLN GLY ASP
SEQRES  24 D  314  LEU GLU ARG ILE ILE ALA ALA MET LYS MET MET LEU GLY
SEQRES  25 D  314  THR ALA
HET    D8F  A 401      17
HET    SO4  A 402       5
HET    EDO  A 403       4
HET    EDO  A 404       4
HET    EDO  A 405       4
HET    EDO  A 406       4
HET    EDO  A 407       4
HET    GOL  A 408       6
HET    GOL  A 409       6
HET    GOL  A 410       6
HET    GOL  A 411       6
HET    GOL  A 412       6
HET    6NA  A 413       8
HET    D8F  B 401      17
HET    EDO  B 402       4
HET    EDO  B 403       4
HET    GOL  B 404       6
HET    GOL  B 405       6
HET    GOL  B 406       6
HET    D8F  C 401      17
HET    EDO  C 402       4
HET    EDO  C 403       4
HET    EDO  C 404       4
HET    GOL  C 405       6
HET    GOL  C 406       6
HET    GOL  C 407       6
HET    GOL  C 408       6
HET    6NA  C 409       8
HET    PGE  C 410      10
HET    D8F  D 401      17
HET    SO4  D 402       5
HET    EDO  D 403       4
HET    EDO  D 404       4
HET    EDO  D 405       4
HET    GOL  D 406       6
HET    GOL  D 407       6
HET    GOL  D 408       6
HET    GOL  D 409       6
HET    NPO  D 410      10
HETNAM     D8F (4-NITROPHENYL) HEXANOATE
HETNAM     SO4 SULFATE ION
HETNAM     EDO 1,2-ETHANEDIOL
HETNAM     GOL GLYCEROL
HETNAM     6NA HEXANOIC ACID
HETNAM     PGE TRIETHYLENE GLYCOL
HETNAM     NPO P-NITROPHENOL
HETSYN     EDO ETHYLENE GLYCOL
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL
FORMUL   5  D8F    4(C12 H15 N O4)
FORMUL   6  SO4    2(O4 S 2-)
FORMUL   7  EDO    13(C2 H6 O2)
FORMUL  12  GOL    16(C3 H8 O3)
FORMUL  17  6NA    2(C6 H12 O2)
FORMUL  33  PGE    C6 H14 O4
FORMUL  43  NPO    C6 H5 N O3
FORMUL  44  HOH   *1409(H2 O)
HELIX    1 AA1 ARG A    8  ALA A   22  1                                  15
HELIX    2 AA2 THR A   25  MET A   29  5                                   5
HELIX    3 AA3 THR A   30  ASP A   46  1                                  17
HELIX    4 AA4 HIS A  100  ASP A  112  1                                  13
HELIX    5 AA5 PRO A  129  SER A  144  1                                  16
HELIX    6 AA6 SER A  145  GLY A  150  5                                   6
HELIX    7 AA7 SER A  162  LYS A  178  1                                  17
HELIX    8 AA8 SER A  200  PHE A  206  1                                   7
HELIX    9 AA9 THR A  213  LYS A  225  1                                  13
HELIX   10 AB1 PHE A  234  GLY A  238  5                                   5
HELIX   11 AB2 ILE A  256  ALA A  270  1                                  15
HELIX   12 AB3 GLY A  285  ILE A  289  5                                   5
HELIX   13 AB4 PRO A  294  GLY A  312  1                                  19
HELIX   14 AB5 ARG B    8  ALA B   22  1                                  15
HELIX   15 AB6 THR B   30  ASP B   46  1                                  17
HELIX   16 AB7 HIS B  100  ASP B  112  1                                  13
HELIX   17 AB8 PRO B  129  SER B  144  1                                  16
HELIX   18 AB9 SER B  145  GLY B  150  5                                   6
HELIX   19 AC1 SER B  162  LYS B  178  1                                  17
HELIX   20 AC2 SER B  200  PHE B  206  1                                   7
HELIX   21 AC3 THR B  213  LYS B  225  1                                  13
HELIX   22 AC4 PHE B  234  GLY B  238  5                                   5
HELIX   23 AC5 ILE B  256  ALA B  270  1                                  15
HELIX   24 AC6 GLY B  285  ILE B  289  5                                   5
HELIX   25 AC7 SER B  295  GLY B  312  1                                  18
HELIX   26 AC8 ARG C    8  ALA C   22  1                                  15
HELIX   27 AC9 THR C   30  ASP C   46  1                                  17
HELIX   28 AD1 HIS C  100  ASP C  112  1                                  13
HELIX   29 AD2 PRO C  129  SER C  144  1                                  16
HELIX   30 AD3 SER C  145  GLY C  150  5                                   6
HELIX   31 AD4 SER C  162  LYS C  178  1                                  17
HELIX   32 AD5 SER C  200  PHE C  206  1                                   7
HELIX   33 AD6 THR C  213  LYS C  225  1                                  13
HELIX   34 AD7 PHE C  234  GLY C  238  5                                   5
HELIX   35 AD8 ILE C  256  ALA C  270  1                                  15
HELIX   36 AD9 GLY C  285  ILE C  289  5                                   5
HELIX   37 AE1 SER C  295  GLY C  312  1                                  18
HELIX   38 AE2 ARG D    8  ALA D   22  1                                  15
HELIX   39 AE3 THR D   25  MET D   29  5                                   5
HELIX   40 AE4 THR D   30  ASP D   46  1                                  17
HELIX   41 AE5 HIS D  100  ASP D  112  1                                  13
HELIX   42 AE6 PRO D  129  SER D  144  1                                  16
HELIX   43 AE7 SER D  145  GLY D  150  5                                   6
HELIX   44 AE8 SER D  162  LYS D  178  1                                  17
HELIX   45 AE9 SER D  200  PHE D  206  1                                   7
HELIX   46 AF1 THR D  213  LYS D  225  1                                  13
HELIX   47 AF2 PHE D  234  GLY D  238  5                                   5
HELIX   48 AF3 ILE D  256  ALA D  270  1                                  15
HELIX   49 AF4 GLY D  285  ILE D  289  5                                   5
HELIX   50 AF5 PRO D  294  GLY D  312  1                                  19
SHEET    1 AA1 6 VAL A  54  CYS A  60  0
SHEET    2 AA1 6 ILE A  67  ASP A  73 -1  O  LEU A  69   N  LEU A  58
SHEET    3 AA1 6 VAL A 115  VAL A 118 -1  O  ALA A 117   N  ARG A  70
SHEET    4 AA1 6 GLY A  81  TYR A  87  1  N  ILE A  84   O  VAL A 116
SHEET    5 AA1 6 ALA A 153  ASP A 161  1  O  ILE A 157   N  THR A  85
SHEET    6 AA1 6 VAL A 188  ILE A 190  1  O  ILE A 190   N  GLY A 160
SHEET    1 AA2 4 THR A 246  ALA A 251  0
SHEET    2 AA2 4 VAL A 274  MET A 279  1  O  MET A 279   N  THR A 250
SHEET    3 AA2 4 VAL B 274  MET B 279 -1  O  TYR B 276   N  VAL A 274
SHEET    4 AA2 4 THR B 246  ALA B 251  1  N  VAL B 248   O  VAL B 275
SHEET    1 AA3 6 VAL B  54  CYS B  60  0
SHEET    2 AA3 6 ILE B  67  ASP B  73 -1  O  LEU B  71   N  ARG B  56
SHEET    3 AA3 6 VAL B 115  VAL B 118 -1  O  VAL B 115   N  TYR B  72
SHEET    4 AA3 6 GLY B  81  TYR B  87  1  N  ILE B  84   O  VAL B 116
SHEET    5 AA3 6 ALA B 153  ASP B 161  1  O  ILE B 157   N  VAL B  83
SHEET    6 AA3 6 VAL B 188  ILE B 190  1  O  ILE B 190   N  GLY B 160
SHEET    1 AA4 6 VAL C  54  CYS C  60  0
SHEET    2 AA4 6 ILE C  67  ASP C  73 -1  O  ILE C  67   N  CYS C  60
SHEET    3 AA4 6 VAL C 115  VAL C 118 -1  O  VAL C 115   N  TYR C  72
SHEET    4 AA4 6 GLY C  81  TYR C  87  1  N  ILE C  84   O  VAL C 116
SHEET    5 AA4 6 ALA C 153  ASP C 161  1  O  ILE C 157   N  VAL C  83
SHEET    6 AA4 6 VAL C 188  ILE C 190  1  O  ILE C 190   N  GLY C 160
SHEET    1 AA5 4 THR C 246  ALA C 251  0
SHEET    2 AA5 4 VAL C 274  MET C 279  1  O  VAL C 275   N  VAL C 248
SHEET    3 AA5 4 VAL D 274  MET D 279 -1  O  VAL D 274   N  TYR C 276
SHEET    4 AA5 4 THR D 246  ALA D 251  1  N  VAL D 248   O  VAL D 275
SHEET    1 AA6 6 VAL D  54  CYS D  60  0
SHEET    2 AA6 6 ILE D  67  ASP D  73 -1  O  LEU D  71   N  ARG D  56
SHEET    3 AA6 6 VAL D 115  VAL D 118 -1  O  VAL D 115   N  TYR D  72
SHEET    4 AA6 6 GLY D  81  TYR D  87  1  N  ILE D  84   O  VAL D 116
SHEET    5 AA6 6 ALA D 153  ASP D 161  1  O  ILE D 157   N  THR D  85
SHEET    6 AA6 6 VAL D 188  ILE D 190  1  O  ILE D 190   N  GLY D 160
LINK         OG  SER A 162                 CAN D8F A 401     1555   1555  1.39
LINK         OG  SER B 162                 CAN D8F B 401     1555   1555  1.38
LINK         OG  SER C 162                 CAN D8F C 401     1555   1555  1.39
LINK         OG  SER D 162                 CAN D8F D 401     1555   1555  1.38
CISPEP   1 ALA A  123    PRO A  124          0         1.77
CISPEP   2 PHE A  128    PRO A  129          0         0.67
CISPEP   3 ALA B  123    PRO B  124          0         1.54
CISPEP   4 PHE B  128    PRO B  129          0         6.12
CISPEP   5 ALA C  123    PRO C  124          0         0.23
CISPEP   6 PHE C  128    PRO C  129          0         4.37
CISPEP   7 ALA D  123    PRO D  124          0         1.50
CISPEP   8 PHE D  128    PRO D  129          0         5.78
SITE     1 AC1 12 TYR A  38  GLY A  90  GLY A  91  SER A 162
SITE     2 AC1 12 ALA A 163  LEU A 193  LEU A 212  PHE A 220
SITE     3 AC1 12 HIS A 284  HOH A 501  HOH A 519  HOH A 538
SITE     1 AC2  4 PRO A  24  HOH A 538  HOH A 548  HOH A 573
SITE     1 AC3  2 PRO A   9  ASP A  10
SITE     1 AC4  3 GLU A  51  LEU A  52  HOH A 517
SITE     1 AC5  4 ARG A 302  HOH A 539  HOH A 734  MET B 309
SITE     1 AC6  3 LYS A 265  HOH A 574  GLU B 269
SITE     1 AC7  2 ASP A 112  HOH A 612
SITE     1 AC8  9 LEU A 193  ASP A 196  ALA A 197  VAL A 198
SITE     2 AC8  9 ASP A 221  ARG A 228  HOH A 502  HOH A 519
SITE     3 AC8  9 HOH A 561
SITE     1 AC9  7 ARG A 257  ARG A 261  GOL A 410  HOH A 564
SITE     2 AC9  7 HOH A 681  HOH B 513  HOH B 562
SITE     1 AD1  2 ARG A 257  GOL A 409
SITE     1 AD2  6 ARG A 140  PRO A 179  ALA A 180  ASP A 181
SITE     2 AD2  6 HOH A 552  HOH A 713
SITE     1 AD3  5 PRO A 124  THR A 222  ALA A 223  HOH A 513
SITE     2 AD3  5 HOH A 607
SITE     1 AD4 10 ASP A  96  ASP A 119  TYR A 120  ARG A 121
SITE     2 AD4 10 HOH A 525  HOH A 534  HOH A 547  HOH A 554
SITE     3 AD4 10 HOH A 679  HOH A 703
SITE     1 AD5  4 GLU B  79  ALA B  80  HOH B 501  HOH B 526
SITE     1 AD6  4 ARG B   8  PRO B   9  ASP B  10  HOH B 614
SITE     1 AD7  4 ALA B 201  ALA B 205  ARG B 257  GOL B 405
SITE     1 AD8  5 HOH A 532  ARG B 257  ARG B 261  GOL B 404
SITE     2 AD8  5 HOH B 519
SITE     1 AD9  8 ASP B 196  ALA B 197  VAL B 198  ILE B 217
SITE     2 AD9  8 ASP B 221  ARG B 228  HOH B 645  HOH B 662
SITE     1 AE1  4 ALA C  64  GLY C  65  ASP C  66  HOH C 624
SITE     1 AE2  3 ARG C 228  HOH C 683  HOH C 790
SITE     1 AE3  4 MET C 309  GLY C 312  HOH C 501  ALA D 305
SITE     1 AE4 10 LEU C 193  ASP C 196  ALA C 197  VAL C 198
SITE     2 AE4 10 ILE C 217  ASP C 221  ARG C 228  HOH C 511
SITE     3 AE4 10 HOH C 573  HOH C 632
SITE     1 AE5  5 ARG C 257  ARG C 261  GOL C 407  HOH C 503
SITE     2 AE5  5 HOH D 582
SITE     1 AE6  2 ARG C 257  GOL C 406
SITE     1 AE7  3 ARG C  75  GLU C  76  SER C  77
SITE     1 AE8  8 ASP C  96  ASP C 119  TYR C 120  ARG C 121
SITE     2 AE8  8 LEU C 122  HOH C 543  HOH C 578  HOH C 838
SITE     1 AE9  7 LYS C 265  ALA C 266  HOH C 597  HOH C 619
SITE     2 AE9  7 HOH C 708  LYS D 265  ALA D 266
SITE     1 AF1  1 ARG D  75
SITE     1 AF2  4 ARG D   8  PRO D   9  ASP D  10  SER D 295
SITE     1 AF3  4 LEU D 122  HIS D 126  EDO D 405  HOH D 703
SITE     1 AF4  5 ASP D 119  TYR D 120  EDO D 404  HOH D 503
SITE     2 AF4  5 HOH D 624
SITE     1 AF5  9 LEU D 193  ASP D 196  ALA D 197  VAL D 198
SITE     2 AF5  9 ILE D 217  ASP D 221  ARG D 228  HOH D 584
SITE     3 AF5  9 HOH D 592
SITE     1 AF6  7 HOH C 544  HOH C 588  ARG D 257  ARG D 261
SITE     2 AF6  7 GOL D 408  HOH D 508  HOH D 654
SITE     1 AF7  4 ALA D 201  ALA D 205  ARG D 257  GOL D 407
SITE     1 AF8  4 ALA D  74  ASP D 112  HOH D 553  HOH D 631
SITE     1 AF9  6 LYS D  12  LEU D  15  GLU D  16  GLY D 209
SITE     2 AF9  6 PHE D 210  HOH D 782
SITE     1 AG1 13 GLY B  90  GLY B  91  ASP B 161  ALA B 163
SITE     2 AG1 13 GLY B 164  GLY B 165  ASN B 166  ILE B 190
SITE     3 AG1 13 PHE B 191  PRO B 192  LEU B 212  HIS B 284
SITE     4 AG1 13 HOH B 645
SITE     1 AG2 15 TYR C  38  GLY C  90  GLY C  91  ASP C 161
SITE     2 AG2 15 ALA C 163  GLY C 164  GLY C 165  ASN C 166
SITE     3 AG2 15 ILE C 190  PHE C 191  PRO C 192  LEU C 212
SITE     4 AG2 15 PHE C 220  HIS C 284  HOH C 748
SITE     1 AG3 15 TYR D  38  GLY D  89  GLY D  90  GLY D  91
SITE     2 AG3 15 ASP D 161  ALA D 163  GLY D 164  GLY D 165
SITE     3 AG3 15 ASN D 166  ILE D 190  PHE D 191  LEU D 193
SITE     4 AG3 15 LEU D 212  HIS D 284  HOH D 584
CRYST1   70.657  129.715  219.464  90.00  90.00  90.00 P 21 2 21    16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.014153  0.000000  0.000000        0.00000
SCALE2      0.000000  0.007709  0.000000        0.00000
SCALE3      0.000000  0.000000  0.004557        0.00000
TER    2272      GLY A 312
TER    4554      GLY B 312
TER    6834      GLY C 312
TER    9118      GLY D 312
MASTER      569    0   39   50   32    0   69    610785    4  266  100
END