longtext: 6t0e-pdb

content
HEADER    HYDROLASE                               03-OCT-19   6T0E
TITLE     THE GLUCURONOYL ESTERASE OTCE15A S267A VARIANT FROM OPITUTUS TERRAE IN
TITLE    2 COMPLEX WITH BENZYL D-GLUCURONOATE AND D-GLUCURONATE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: GLUCURONOYL ESTERASE OTCE15A;
COMPND   3 CHAIN: A, B;
COMPND   4 ENGINEERED: YES;
COMPND   5 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: OPITUTUS TERRAE (STRAIN DSM 11246 / JCM 15787 /
SOURCE   3 PB90-1);
SOURCE   4 ORGANISM_TAXID: 452637;
SOURCE   5 STRAIN: DSM 11246 / JCM 15787 / PB90-1;
SOURCE   6 GENE: OTER_0116;
SOURCE   7 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE   8 EXPRESSION_SYSTEM_TAXID: 469008
KEYWDS    ESTERASE, COMPLEX, BIOMASS, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    S.MAZURKEWICH,J.C.NAVARRO POULSEN,J.LARSBRINK,L.LO LEGGIO
REVDAT   1   27-NOV-19 6T0E    0
JRNL        AUTH   S.MAZURKEWICH,J.C.NAVARRO POULSEN,J.LARSBRINK,L.LO LEGGIO
JRNL        TITL   THE GLUCURONOYL ESTERASE OTCE15A S267A VARIANT FROM OPITUTUS
JRNL        TITL 2 TERRAE IN COMPLEX WITH BENZYL D-GLUCURONOATE AND
JRNL        TITL 3 D-GLUCURONATE
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.89 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.15.2_3472
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.89
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 44.45
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.330
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.6
REMARK   3   NUMBER OF REFLECTIONS             : 63362
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.164
REMARK   3   R VALUE            (WORKING SET) : 0.162
REMARK   3   FREE R VALUE                     : 0.216
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 3.140
REMARK   3   FREE R VALUE TEST SET COUNT      : 1988
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 44.4500 -  4.5500    1.00     4725   155  0.1677 0.2271
REMARK   3     2  4.5500 -  3.6100    1.00     4528   147  0.1252 0.1629
REMARK   3     3  3.6100 -  3.1600    1.00     4476   145  0.1362 0.1790
REMARK   3     4  3.1600 -  2.8700    1.00     4448   145  0.1491 0.1979
REMARK   3     5  2.8700 -  2.6600    0.99     4402   141  0.1572 0.2532
REMARK   3     6  2.6600 -  2.5100    1.00     4400   143  0.1623 0.2347
REMARK   3     7  2.5100 -  2.3800    0.99     4384   142  0.1678 0.2110
REMARK   3     8  2.3800 -  2.2800    1.00     4429   143  0.1682 0.2099
REMARK   3     9  2.2800 -  2.1900    1.00     4376   141  0.1783 0.2481
REMARK   3    10  2.1900 -  2.1100    1.00     4397   143  0.1915 0.2917
REMARK   3    11  2.1100 -  2.0500    1.00     4379   141  0.2146 0.2487
REMARK   3    12  2.0500 -  1.9900    1.00     4360   142  0.2485 0.2729
REMARK   3    13  1.9900 -  1.9400    1.00     4406   143  0.2804 0.3733
REMARK   3    14  1.9400 -  1.8900    0.84     3664   117  0.3399 0.3654
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : NULL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.253
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 22.558
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 33.05
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 39.74
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.011           6649
REMARK   3   ANGLE     :  1.121           8976
REMARK   3   CHIRALITY :  0.062            930
REMARK   3   PLANARITY :  0.008           1175
REMARK   3   DIHEDRAL  : 22.805           2389
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 6T0E COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 03-OCT-19.
REMARK 100 THE DEPOSITION ID IS D_1292104635.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 09-DEC-18
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 8.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : ESRF
REMARK 200  BEAMLINE                       : MASSIF-3
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9677
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER X 4M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XDS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 63514
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.890
REMARK 200  RESOLUTION RANGE LOW       (A) : 44.450
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.0
REMARK 200  DATA REDUNDANCY                : 13.60
REMARK 200  R MERGE                    (I) : 0.15890
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 12.2500
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.89
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.96
REMARK 200  COMPLETENESS FOR SHELL     (%) : 92.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 13.40
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 0.840
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHENIX 1.15.2_3472
REMARK 200 STARTING MODEL: 6GS0
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 42.39
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.14
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: ENZYME MIXED 50/50 WITH RESERVOIR
REMARK 280  SOLUTION CONTAINING JCSG+ SCREEN SOLUTION G4: 0.2 M
REMARK 280  TRIMETHYLAMINE N-OXIDE, 0.1 M TRIS PH 8.5, AND 20 % W/V PEG 2000
REMARK 280  MME, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       25.85950
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       86.95500
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       43.80550
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       86.95500
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       25.85950
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       43.80550
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A    12
REMARK 465     GLY A    13
REMARK 465     SER A    14
REMARK 465     SER A    15
REMARK 465     HIS A    16
REMARK 465     HIS A    17
REMARK 465     HIS A    18
REMARK 465     HIS A    19
REMARK 465     HIS A    20
REMARK 465     HIS A    21
REMARK 465     SER A    22
REMARK 465     SER A    23
REMARK 465     GLU A    24
REMARK 465     ASN A    25
REMARK 465     LEU A    26
REMARK 465     TYR A    27
REMARK 465     PHE A    28
REMARK 465     GLN A    29
REMARK 465     GLY A    30
REMARK 465     HIS A    31
REMARK 465     SER A    32
REMARK 465     ALA A    33
REMARK 465     TYR A    34
REMARK 465     THR A    35
REMARK 465     SER A   428
REMARK 465     ALA A   429
REMARK 465     LEU A   430
REMARK 465     PRO A   431
REMARK 465     ALA A   432
REMARK 465     MET B    12
REMARK 465     GLY B    13
REMARK 465     SER B    14
REMARK 465     SER B    15
REMARK 465     HIS B    16
REMARK 465     HIS B    17
REMARK 465     HIS B    18
REMARK 465     HIS B    19
REMARK 465     HIS B    20
REMARK 465     HIS B    21
REMARK 465     SER B    22
REMARK 465     SER B    23
REMARK 465     GLU B    24
REMARK 465     ASN B    25
REMARK 465     LEU B    26
REMARK 465     TYR B    27
REMARK 465     PHE B    28
REMARK 465     GLN B    29
REMARK 465     GLY B    30
REMARK 465     HIS B    31
REMARK 465     SER B    32
REMARK 465     ALA B    33
REMARK 465     TYR B    34
REMARK 465     ALA B   429
REMARK 465     LEU B   430
REMARK 465     PRO B   431
REMARK 465     ALA B   432
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ARG A  57      -54.56   -123.16
REMARK 500    VAL A  70      -71.41   -120.06
REMARK 500    ASP A 150      103.35    -30.33
REMARK 500    ALA A 267     -126.20     57.65
REMARK 500    HIS A 328       43.23   -150.34
REMARK 500    ASP A 356       65.98   -104.23
REMARK 500    ASP A 357       11.10    -68.49
REMARK 500    TRP A 358      -30.47   -171.52
REMARK 500    ASP A 360       75.18     59.50
REMARK 500    LEU A 426       60.04   -108.26
REMARK 500    LEU B  40       33.06    -95.68
REMARK 500    VAL B  70      -69.34   -122.12
REMARK 500    ASP B 150      108.37    -35.44
REMARK 500    ALA B 267     -124.34     58.31
REMARK 500    ILE B 302       40.26     37.93
REMARK 500    ASP B 327       29.99     49.88
REMARK 500    HIS B 328       46.60   -152.59
REMARK 500    ASP B 356       57.16   -105.28
REMARK 500    TRP B 358      -22.82     70.57
REMARK 500    ASP B 360       78.20     59.32
REMARK 500
REMARK 500 REMARK: NULL
REMARK 620
REMARK 620 METAL COORDINATION
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 620
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL
REMARK 620                              MG A 532  MG
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 HIS A 303   O
REMARK 620 2 GLU A 305   O   101.0
REMARK 620 3 HOH A 739   O    99.6  92.3
REMARK 620 4 HOH A 781   O    99.0  83.8 161.3
REMARK 620 5 HOH A 654   O    90.6 159.0 103.0  77.1
REMARK 620 N                    1     2     3     4
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GCU A 501
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 502
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue M55 A 503
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PG4 A 504
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 505
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 506
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 507
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 508
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 509
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 510
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 511
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 512
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 513
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 514
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 515
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 516
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 517
REMARK 800
REMARK 800 SITE_IDENTIFIER: AD9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 518
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 519
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 520
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 521
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 522
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 523
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PEG A 524
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PEG A 525
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PEG A 526
REMARK 800
REMARK 800 SITE_IDENTIFIER: AE9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PEG A 527
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PGE A 528
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PGE A 529
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PGE A 530
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PGE A 531
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue MG A 532
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue TMO A 533
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue TMO A 534
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue TMO A 535
REMARK 800
REMARK 800 SITE_IDENTIFIER: AF9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue DMS A 536
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue DMS A 537
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GCU B 501
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 502
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 503
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 504
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 505
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 506
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 507
REMARK 800
REMARK 800 SITE_IDENTIFIER: AG9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 508
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 509
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 510
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 511
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 512
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 513
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 514
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 515
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 516
REMARK 800
REMARK 800 SITE_IDENTIFIER: AH9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PEG B 517
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PEG B 518
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue PGE B 519
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue TMO B 520
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue TMO B 521
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue TMO B 522
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue TMO B 523
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue DMS B 524
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue DMS B 525
REMARK 800
REMARK 800 SITE_IDENTIFIER: AI9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue DMS B 526
DBREF  6T0E A   33   432  UNP    B1ZMF4   B1ZMF4_OPITP    33    432
DBREF  6T0E B   33   432  UNP    B1ZMF4   B1ZMF4_OPITP    33    432
SEQADV 6T0E MET A   12  UNP  B1ZMF4              INITIATING METHIONINE
SEQADV 6T0E GLY A   13  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER A   14  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER A   15  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS A   16  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS A   17  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS A   18  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS A   19  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS A   20  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS A   21  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER A   22  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER A   23  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E GLU A   24  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E ASN A   25  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E LEU A   26  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E TYR A   27  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E PHE A   28  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E GLN A   29  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E GLY A   30  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS A   31  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER A   32  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E ALA A  267  UNP  B1ZMF4    SER   267 ENGINEERED MUTATION
SEQADV 6T0E MET B   12  UNP  B1ZMF4              INITIATING METHIONINE
SEQADV 6T0E GLY B   13  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER B   14  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER B   15  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS B   16  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS B   17  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS B   18  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS B   19  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS B   20  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS B   21  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER B   22  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER B   23  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E GLU B   24  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E ASN B   25  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E LEU B   26  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E TYR B   27  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E PHE B   28  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E GLN B   29  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E GLY B   30  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E HIS B   31  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E SER B   32  UNP  B1ZMF4              EXPRESSION TAG
SEQADV 6T0E ALA B  267  UNP  B1ZMF4    SER   267 ENGINEERED MUTATION
SEQRES   1 A  421  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLU
SEQRES   2 A  421  ASN LEU TYR PHE GLN GLY HIS SER ALA TYR THR LEU PRO
SEQRES   3 A  421  ASP PRO LEU VAL GLY ALA ASP GLY THR ARG VAL HIS ASP
SEQRES   4 A  421  ARG ALA THR TRP GLN HIS ARG ARG ARG PRO GLU LEU LEU
SEQRES   5 A  421  GLN LEU PHE ALA ARG GLU VAL TYR GLY ARG THR PRO LEU
SEQRES   6 A  421  GLY ARG PRO GLU GLY MET VAL PHE LYS VAL THR THR MET
SEQRES   7 A  421  GLU HIS ALA ALA LEU GLY GLY ALA ALA THR ARG LYS GLU
SEQRES   8 A  421  VAL THR VAL ARG PHE GLY ARG ASP PRO ASN ALA PRO SER
SEQRES   9 A  421  MET GLN LEU LEU LEU TYR VAL PRO ASN ALA VAL ILE ALA
SEQRES  10 A  421  ARG ALA GLU ARG ALA PRO VAL PHE LEU GLY LEU ASN PHE
SEQRES  11 A  421  TYR GLY ASN HIS THR VAL HIS THR ASP PRO ALA ILE ALA
SEQRES  12 A  421  LEU SER ALA ARG TRP ILE PRO ALA GLU ALA PRO ASN GLY
SEQRES  13 A  421  ALA ASN HIS ARG ALA THR GLU ALA ALA ARG GLY SER ASP
SEQRES  14 A  421  ALA GLN LYS TRP PRO VAL GLU GLN ILE LEU ALA ARG GLY
SEQRES  15 A  421  TYR ALA VAL ALA THR VAL TYR CYS GLY ASP LEU CYS PRO
SEQRES  16 A  421  ASP ARG PRO ASP GLY LEU ASN ALA SER VAL ALA SER TRP
SEQRES  17 A  421  LEU ASP ALA ALA ALA GLY ASP GLN ARG ALA PRO ASP ALA
SEQRES  18 A  421  TRP GLY ALA ILE GLY VAL TRP ALA TRP GLY LEU SER ARG
SEQRES  19 A  421  ALA LEU ASP TYR LEU GLU THR ASP PRO LEU VAL ASP ALA
SEQRES  20 A  421  SER ARG VAL ALA VAL HIS GLY HIS ALA ARG LEU GLY LYS
SEQRES  21 A  421  ALA ALA LEU TRP ALA GLY ALA GLN ASP ASP ARG PHE ALA
SEQRES  22 A  421  LEU VAL ILE SER ASN GLU SER GLY CYS GLY GLY ALA ALA
SEQRES  23 A  421  LEU SER LYS ARG ILE HIS GLY GLU THR VAL ALA ARG ILE
SEQRES  24 A  421  ASN THR VAL PHE PRO HIS TRP PHE ALA ARG ASN PHE ARG
SEQRES  25 A  421  ARG TYR ASP ASP HIS GLU GLU ALA LEU PRO VAL ASP GLN
SEQRES  26 A  421  HIS GLU LEU LEU ALA LEU VAL ALA PRO ARG PRO LEU TYR
SEQRES  27 A  421  VAL ALA SER ALA GLU ASP ASP ASP TRP ALA ASP PRO ARG
SEQRES  28 A  421  GLY GLU PHE LEU ALA VAL LYS ALA ALA GLU PRO VAL PHE
SEQRES  29 A  421  ARG LEU PHE GLY GLN THR GLY PRO SER GLY GLU ASP VAL
SEQRES  30 A  421  PRO ARG VAL ASN GLU PRO SER GLY GLY ALA LEU ARG TYR
SEQRES  31 A  421  HIS ILE ARG PRO GLY PRO HIS GLY MET THR ALA GLN ASP
SEQRES  32 A  421  TRP ALA PHE TYR LEU ALA PHE ALA ASP GLU TRP LEU LYS
SEQRES  33 A  421  SER ALA LEU PRO ALA
SEQRES   1 B  421  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLU
SEQRES   2 B  421  ASN LEU TYR PHE GLN GLY HIS SER ALA TYR THR LEU PRO
SEQRES   3 B  421  ASP PRO LEU VAL GLY ALA ASP GLY THR ARG VAL HIS ASP
SEQRES   4 B  421  ARG ALA THR TRP GLN HIS ARG ARG ARG PRO GLU LEU LEU
SEQRES   5 B  421  GLN LEU PHE ALA ARG GLU VAL TYR GLY ARG THR PRO LEU
SEQRES   6 B  421  GLY ARG PRO GLU GLY MET VAL PHE LYS VAL THR THR MET
SEQRES   7 B  421  GLU HIS ALA ALA LEU GLY GLY ALA ALA THR ARG LYS GLU
SEQRES   8 B  421  VAL THR VAL ARG PHE GLY ARG ASP PRO ASN ALA PRO SER
SEQRES   9 B  421  MET GLN LEU LEU LEU TYR VAL PRO ASN ALA VAL ILE ALA
SEQRES  10 B  421  ARG ALA GLU ARG ALA PRO VAL PHE LEU GLY LEU ASN PHE
SEQRES  11 B  421  TYR GLY ASN HIS THR VAL HIS THR ASP PRO ALA ILE ALA
SEQRES  12 B  421  LEU SER ALA ARG TRP ILE PRO ALA GLU ALA PRO ASN GLY
SEQRES  13 B  421  ALA ASN HIS ARG ALA THR GLU ALA ALA ARG GLY SER ASP
SEQRES  14 B  421  ALA GLN LYS TRP PRO VAL GLU GLN ILE LEU ALA ARG GLY
SEQRES  15 B  421  TYR ALA VAL ALA THR VAL TYR CYS GLY ASP LEU CYS PRO
SEQRES  16 B  421  ASP ARG PRO ASP GLY LEU ASN ALA SER VAL ALA SER TRP
SEQRES  17 B  421  LEU ASP ALA ALA ALA GLY ASP GLN ARG ALA PRO ASP ALA
SEQRES  18 B  421  TRP GLY ALA ILE GLY VAL TRP ALA TRP GLY LEU SER ARG
SEQRES  19 B  421  ALA LEU ASP TYR LEU GLU THR ASP PRO LEU VAL ASP ALA
SEQRES  20 B  421  SER ARG VAL ALA VAL HIS GLY HIS ALA ARG LEU GLY LYS
SEQRES  21 B  421  ALA ALA LEU TRP ALA GLY ALA GLN ASP ASP ARG PHE ALA
SEQRES  22 B  421  LEU VAL ILE SER ASN GLU SER GLY CYS GLY GLY ALA ALA
SEQRES  23 B  421  LEU SER LYS ARG ILE HIS GLY GLU THR VAL ALA ARG ILE
SEQRES  24 B  421  ASN THR VAL PHE PRO HIS TRP PHE ALA ARG ASN PHE ARG
SEQRES  25 B  421  ARG TYR ASP ASP HIS GLU GLU ALA LEU PRO VAL ASP GLN
SEQRES  26 B  421  HIS GLU LEU LEU ALA LEU VAL ALA PRO ARG PRO LEU TYR
SEQRES  27 B  421  VAL ALA SER ALA GLU ASP ASP ASP TRP ALA ASP PRO ARG
SEQRES  28 B  421  GLY GLU PHE LEU ALA VAL LYS ALA ALA GLU PRO VAL PHE
SEQRES  29 B  421  ARG LEU PHE GLY GLN THR GLY PRO SER GLY GLU ASP VAL
SEQRES  30 B  421  PRO ARG VAL ASN GLU PRO SER GLY GLY ALA LEU ARG TYR
SEQRES  31 B  421  HIS ILE ARG PRO GLY PRO HIS GLY MET THR ALA GLN ASP
SEQRES  32 B  421  TRP ALA PHE TYR LEU ALA PHE ALA ASP GLU TRP LEU LYS
SEQRES  33 B  421  SER ALA LEU PRO ALA
HET    GCU  A 501      22
HET     CL  A 502       1
HET    M55  A 503      36
HET    PG4  A 504      31
HET    EDO  A 505      10
HET    EDO  A 506      10
HET    EDO  A 507      10
HET    EDO  A 508      10
HET    EDO  A 509      10
HET    EDO  A 510      10
HET    EDO  A 511      10
HET    EDO  A 512      10
HET    EDO  A 513      10
HET    EDO  A 514      10
HET    EDO  A 515      10
HET    EDO  A 516      10
HET    EDO  A 517      10
HET    EDO  A 518      10
HET    EDO  A 519      10
HET    EDO  A 520      10
HET    EDO  A 521      10
HET    EDO  A 522      10
HET    EDO  A 523      10
HET    PEG  A 524      17
HET    PEG  A 525      17
HET    PEG  A 526      17
HET    PEG  A 527      17
HET    PGE  A 528      24
HET    PGE  A 529      24
HET    PGE  A 530      24
HET    PGE  A 531      24
HET     MG  A 532       1
HET    TMO  A 533      14
HET    TMO  A 534      14
HET    TMO  A 535      14
HET    DMS  A 536      10
HET    DMS  A 537      10
HET    GCU  B 501      22
HET    EDO  B 502      10
HET    EDO  B 503      10
HET    EDO  B 504      10
HET    EDO  B 505      10
HET    EDO  B 506      10
HET    EDO  B 507      10
HET    EDO  B 508      10
HET    EDO  B 509      10
HET    EDO  B 510      10
HET    EDO  B 511      10
HET    EDO  B 512      10
HET    EDO  B 513      10
HET    EDO  B 514      10
HET    EDO  B 515      10
HET    EDO  B 516      10
HET    PEG  B 517      17
HET    PEG  B 518      17
HET    PGE  B 519      24
HET    TMO  B 520      14
HET    TMO  B 521      14
HET    TMO  B 522      14
HET    TMO  B 523      14
HET    DMS  B 524      10
HET    DMS  B 525      10
HET    DMS  B 526      10
HETNAM     GCU D-GLUCURONIC ACID
HETNAM      CL CHLORIDE ION
HETNAM     M55 BENZYL D-GLUCURONOATE
HETNAM     PG4 TETRAETHYLENE GLYCOL
HETNAM     EDO 1,2-ETHANEDIOL
HETNAM     PEG DI(HYDROXYETHYL)ETHER
HETNAM     PGE TRIETHYLENE GLYCOL
HETNAM      MG MAGNESIUM ION
HETNAM     TMO TRIMETHYLAMINE OXIDE
HETNAM     DMS DIMETHYL SULFOXIDE
HETSYN     EDO ETHYLENE GLYCOL
FORMUL   3  GCU    2(C6 H10 O7)
FORMUL   4   CL    CL 1-
FORMUL   5  M55    C13 H16 O7
FORMUL   6  PG4    C8 H18 O5
FORMUL   7  EDO    34(C2 H6 O2)
FORMUL  26  PEG    6(C4 H10 O3)
FORMUL  30  PGE    5(C6 H14 O4)
FORMUL  34   MG    MG 2+
FORMUL  35  TMO    7(C3 H9 N O)
FORMUL  38  DMS    5(C2 H6 O S)
FORMUL  66  HOH   *412(H2 O)
HELIX    1 AA1 ASP A   50  ARG A   57  1                                   8
HELIX    2 AA2 ARG A   57  VAL A   70  1                                  14
HELIX    3 AA3 LEU A   94  GLY A   96  5                                   3
HELIX    4 AA4 ASN A  124  ARG A  129  1                                   6
HELIX    5 AA5 GLY A  143  VAL A  147  5                                   5
HELIX    6 AA6 THR A  173  ARG A  177  5                                   5
HELIX    7 AA7 ASP A  180  TRP A  184  5                                   5
HELIX    8 AA8 PRO A  185  ARG A  192  1                                   8
HELIX    9 AA9 GLY A  202  LEU A  204  5                                   3
HELIX   10 AB1 ASP A  210  ALA A  214  5                                   5
HELIX   11 AB2 SER A  215  ASP A  221  1                                   7
HELIX   12 AB3 ALA A  224  ARG A  228  5                                   5
HELIX   13 AB4 GLY A  234  GLU A  251  1                                  18
HELIX   14 AB5 ALA A  267  ASP A  280  1                                  14
HELIX   15 AB6 THR A  306  PHE A  314  1                                   9
HELIX   16 AB7 ALA A  319  ASP A  326  5                                   8
HELIX   17 AB8 HIS A  328  LEU A  332  5                                   5
HELIX   18 AB9 ASP A  335  LEU A  342  1                                   8
HELIX   19 AC1 ASP A  356  ALA A  359  5                                   4
HELIX   20 AC2 ASP A  360  PHE A  378  1                                  19
HELIX   21 AC3 THR A  411  LEU A  426  1                                  16
HELIX   22 AC4 ASP B   50  ARG B   57  1                                   8
HELIX   23 AC5 ARG B   57  VAL B   70  1                                  14
HELIX   24 AC6 ASN B  124  ARG B  129  1                                   6
HELIX   25 AC7 GLY B  143  VAL B  147  5                                   5
HELIX   26 AC8 THR B  173  ARG B  177  5                                   5
HELIX   27 AC9 ASP B  180  TRP B  184  5                                   5
HELIX   28 AD1 PRO B  185  ARG B  192  1                                   8
HELIX   29 AD2 CYS B  201  CYS B  205  1                                   5
HELIX   30 AD3 ASP B  210  ALA B  214  5                                   5
HELIX   31 AD4 SER B  215  ASP B  221  1                                   7
HELIX   32 AD5 ALA B  224  ARG B  228  5                                   5
HELIX   33 AD6 GLY B  234  GLU B  251  1                                  18
HELIX   34 AD7 ALA B  267  ASP B  280  1                                  14
HELIX   35 AD8 LEU B  298  ILE B  302  5                                   5
HELIX   36 AD9 THR B  306  PHE B  314  1                                   9
HELIX   37 AE1 ALA B  319  ASP B  326  5                                   8
HELIX   38 AE2 HIS B  328  LEU B  332  5                                   5
HELIX   39 AE3 ASP B  335  LEU B  342  1                                   8
HELIX   40 AE4 ASP B  360  PHE B  378  1                                  19
HELIX   41 AE5 THR B  411  LEU B  426  1                                  16
SHEET    1 AA1 9 VAL A  83  ALA A  93  0
SHEET    2 AA1 9 ALA A  98  ARG A 106 -1  O  ARG A 106   N  VAL A  83
SHEET    3 AA1 9 SER A 115  PRO A 123 -1  O  LEU A 118   N  VAL A 103
SHEET    4 AA1 9 ALA A 195  TYR A 200 -1  O  THR A 198   N  LEU A 119
SHEET    5 AA1 9 ALA A 133  ASN A 140  1  N  PRO A 134   O  ALA A 195
SHEET    6 AA1 9 VAL A 256  HIS A 266  1  O  ALA A 262   N  VAL A 135
SHEET    7 AA1 9 LEU A 285  ASN A 289  1  O  ASN A 289   N  GLY A 265
SHEET    8 AA1 9 LEU A 348  ALA A 353  1  O  TYR A 349   N  SER A 288
SHEET    9 AA1 9 LEU A 399  ARG A 404  1  O  ARG A 400   N  LEU A 348
SHEET    1 AA210 VAL B  83  ALA B  93  0
SHEET    2 AA210 ALA B  98  ARG B 106 -1  O  ARG B 100   N  GLU B  90
SHEET    3 AA210 SER B 115  PRO B 123 -1  O  LEU B 118   N  VAL B 103
SHEET    4 AA210 ALA B 195  TYR B 200 -1  O  THR B 198   N  LEU B 119
SHEET    5 AA210 ALA B 133  ASN B 140  1  N  PRO B 134   O  ALA B 195
SHEET    6 AA210 VAL B 256  HIS B 266  1  O  ALA B 262   N  VAL B 135
SHEET    7 AA210 LEU B 285  ASN B 289  1  O  ASN B 289   N  GLY B 265
SHEET    8 AA210 LEU B 348  ALA B 353  1  O  TYR B 349   N  SER B 288
SHEET    9 AA210 LEU B 399  ARG B 404  1  O  ARG B 400   N  VAL B 350
SHEET   10 AA210 SER B 395  GLY B 396 -1  N  SER B 395   O  TYR B 401
LINK         O   HIS A 303                MG    MG A 532     1555   1555  2.23
LINK         O   GLU A 305                MG    MG A 532     1555   1555  2.38
LINK        MG    MG A 532                 O   HOH A 739     1555   1555  2.60
LINK        MG    MG A 532                 O   HOH A 781     1555   1555  2.54
LINK        MG    MG A 532                 O   HOH A 654     1555   1555  2.36
CISPEP   1 ALA A  344    PRO A  345          0         1.34
CISPEP   2 ALA B  344    PRO B  345          0         2.58
SITE     1 AC1  8 ALA A 267  ARG A 268  LYS A 271  GLU A 305
SITE     2 AC1  8 PHE A 314  TRP A 358  HIS A 408  HOH A 650
SITE     1 AC2  5 GLN A 188  ARG A 192  HOH A 784  GLN B 188
SITE     2 AC2  5 ARG B 192
SITE     1 AC3 11 ASP A 207  PRO A 209  ARG A 268  THR A 312
SITE     2 AC3 11 VAL A 313  PHE A 314  HIS A 316  EDO A 519
SITE     3 AC3 11 ALA B  52  HIS B  56  ARG B  57
SITE     1 AC4  6 ARG A 192  HOH A 725  HOH A 765  ALA B 191
SITE     2 AC4  6 ARG B 192  HOH B 675
SITE     1 AC5  6 LEU A 212  ASP A 221  ALA A 223  ARG A 228
SITE     2 AC5  6 HOH A 602  HOH A 686
SITE     1 AC6  6 ALA A  43  ARG A  58  LEU A  65  PRO A 333
SITE     2 AC6  6 HOH A 646  HOH A 653
SITE     1 AC7  2 HIS A  91  ASN A 124
SITE     1 AC8  2 GLN A 182  EDO B 516
SITE     1 AC9  5 ARG A 192  TYR A 194  LEU A 426  LYS A 427
SITE     2 AC9  5 HOH A 622
SITE     1 AD1  5 ARG A  78  PRO A  79  MET A  82  ASP A 387
SITE     2 AD1  5 HOH A 794
SITE     1 AD2  1 GLU A 131
SITE     1 AD3  3 ASN A 321  ARG A 324  EDO A 513
SITE     1 AD4  4 ARG A  68  GLU A  69  ASN A 321  EDO A 512
SITE     1 AD5  2 SER A 218  ALA A 222
SITE     1 AD6  4 ARG A 132  GLU A 251  THR A 252  PRO A 254
SITE     1 AD7  3 ASN A 112  SER A 215  HOH A 722
SITE     1 AD8  1 ARG A 208
SITE     1 AD9  4 PRO A 373  HOH A 604  HOH A 618  HOH A 749
SITE     1 AE1  3 GLU A 163  ARG A 268  M55 A 503
SITE     1 AE2  4 GLU A 372  ARG A 376  HOH A 611  HOH A 625
SITE     1 AE3  6 ASP A  44  PRO A  79  GLU A  80  TRP A 219
SITE     2 AE3  6 TMO A 533  HOH A 601
SITE     1 AE4  2 VAL A  86  PGE A 529
SITE     1 AE5  1 ASP A  50
SITE     1 AE6  6 ALA A  93  LEU A  94  ARG A 100  ASP A 150
SITE     2 AE6  6 HOH A 636  HOH A 796
SITE     1 AE7  5 ARG A  68  GLU A  69  TYR A 325  ALA A 331
SITE     2 AE7  5 PRO A 333
SITE     1 AE8  6 ASP A 110  ARG A 301  ILE A 302  HIS A 303
SITE     2 AE8  6 TMO A 533  HOH A 734
SITE     1 AE9  5 GLN A 182  PRO A 407  THR A 411  LEU B  94
SITE     2 AE9  5 GLU B 187
SITE     1 AF1  8 PRO A 373  ARG A 376  HOH A 625  HOH A 775
SITE     2 AF1  8 ASP B 210  ASN B 213  ALA B 224  ASP B 226
SITE     1 AF2  7 LYS A  85  GLU A 354  ASP A 355  PRO A 405
SITE     2 AF2  7 EDO A 522  ALA B  92  GLY B  96
SITE     1 AF3  8 THR A  74  LEU A  76  GLY A  77  ARG A 245
SITE     2 AF3  8 GLN A 279  ASP A 280  TMO A 534  HOH A 672
SITE     1 AF4  6 LEU A  36  PRO A  39  ARG A  68  ARG A  73
SITE     2 AF4  6 ALA A 370  HOH A 694
SITE     1 AF5  5 HIS A 303  GLU A 305  HOH A 654  HOH A 739
SITE     2 AF5  5 HOH A 781
SITE     1 AF6  6 ASP A  44  THR A  46  ARG A  57  TRP A 219
SITE     2 AF6  6 EDO A 521  PEG A 526
SITE     1 AF7  5 ALA A  67  THR A  74  GLN A 279  PGE A 530
SITE     2 AF7  5 HOH A 789
SITE     1 AF8  2 PRO A 345  PRO A 347
SITE     1 AF9  6 VAL A  86  MET A  89  HOH A 680  HIS B  91
SITE     2 AF9  6 THR B  99  ILE B 127
SITE     1 AG1  4 ARG A 376  ARG B 208  HOH B 694  HOH B 784
SITE     1 AG2  9 ALA B 267  ARG B 268  LYS B 271  GLU B 305
SITE     2 AG2  9 ILE B 310  TRP B 358  HIS B 408  PGE B 519
SITE     3 AG2  9 HOH B 611
SITE     1 AG3  2 GLU B 424  TRP B 425
SITE     1 AG4  5 ASP B 221  ALA B 223  GLY B 225  ARG B 228
SITE     2 AG4  5 HOH B 655
SITE     1 AG5  6 TYR B 349  PRO B 394  ARG B 400  PHE B 417
SITE     2 AG5  6 ALA B 420  PHE B 421
SITE     1 AG6  1 ASN B 166
SITE     1 AG7  4 ARG B  68  GLU B  69  TYR B 325  HOH B 617
SITE     1 AG8  8 ARG B 132  LEU B 250  GLU B 251  ASP B 253
SITE     2 AG8  8 VAL B 256  ASP B 257  ALA B 258  HOH B 636
SITE     1 AG9  4 ARG B  59  ALA B 278  GLN B 279  ASP B 281
SITE     1 AH1  4 PRO B 394  SER B 395  GLY B 396  ARG B 400
SITE     1 AH2  3 ARG B 324  HIS B 328  ALA B 331
SITE     1 AH3  4 VAL B  41  GLY B  42  ARG B  58  HOH B 642
SITE     1 AH4  3 GLU B 354  VAL B 388  PRO B 389
SITE     1 AH5  2 THR B  35  HOH B 657
SITE     1 AH6  3 LYS B 101  TYR B 249  THR B 252
SITE     1 AH7  3 GLU B  69  ARG B 324  HOH B 654
SITE     1 AH8  7 EDO A 508  HOH A 606  PRO B 185  VAL B 186
SITE     2 AH8  7 GLU B 187  GLN B 188  HOH B 603
SITE     1 AH9  9 GLU A 187  GLN B 182  LYS B 183  GLY B 409
SITE     2 AH9  9 MET B 410  THR B 411  ALA B 412  HOH B 605
SITE     3 AH9  9 HOH B 765
SITE     1 AI1  8 PRO B 134  ARG B 192  GLY B 193  TYR B 194
SITE     2 AI1  8 LEU B 426  LYS B 427  HOH B 643  HOH B 690
SITE     1 AI2  9 TYR B 142  PRO B 165  SER B 179  ASP B 180
SITE     2 AI2  9 GLN B 182  LYS B 183  HIS B 266  HIS B 408
SITE     3 AI2  9 GCU B 501
SITE     1 AI3 10 GLY A 397  GLU A 424  TRP A 425  HOH A 619
SITE     2 AI3 10 ALA B 157  ARG B 158  TRP B 159  ARG B 171
SITE     3 AI3 10 HOH B 644  HOH B 713
SITE     1 AI4  3 ASP A 357  TRP A 358  ARG B  51
SITE     1 AI5  4 TRP B 219  LEU B 220  ASP B 221  ALA B 222
SITE     1 AI6  5 ALA B  67  ARG B  73  THR B  74  GLN B 279
SITE     2 AI6  5 HOH B 745
SITE     1 AI7  5 SER B 244  ARG B 245  GLN B 279  ASP B 280
SITE     2 AI7  5 HOH B 734
SITE     1 AI8  4 VAL B  86  THR B  87  THR B  88  MET B  89
SITE     1 AI9  2 GLU B  80  TRP B 219
CRYST1   51.719   87.611  173.910  90.00  90.00  90.00 P 21 21 21    8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.019335  0.000000  0.000000        0.00000
SCALE2      0.000000  0.011414  0.000000        0.00000
SCALE3      0.000000  0.000000  0.005750        0.00000
TER    6028      LYS A 427
TER   12065      SER B 428
MASTER      576    0   63   41   19    0  100    6 6837    2  828   66
END