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HEADER HYDROLASE 18-AUG-20 7A3G
TITLE CRYSTAL STRUCTURE OF DPP8 IN COMPLEX WITH A 4-OXO-B-LACTAM BASED
TITLE 2 INHIBITOR, 91
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: DIPEPTIDYL PEPTIDASE 8;
COMPND 3 CHAIN: B, A;
COMPND 4 SYNONYM: DP8,DIPEPTIDYL PEPTIDASE IV-RELATED PROTEIN 1,DPRP-1,
COMPND 5 DIPEPTIDYL PEPTIDASE VIII,DPP VIII,PROLYL DIPEPTIDASE DPP8;
COMPND 6 EC: 3.4.14.5;
COMPND 7 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 GENE: DPP8, DPRP1, MSTP097, MSTP135, MSTP141;
SOURCE 6 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;
SOURCE 7 EXPRESSION_SYSTEM_COMMON: FALL ARMYWORM;
SOURCE 8 EXPRESSION_SYSTEM_TAXID: 7108
KEYWDS DPP8, PROTEASE, HYDROLASE
EXPDTA X-RAY DIFFRACTION
AUTHOR B.H.ROSS,R.HUBER
REVDAT 1 30-JUN-21 7A3G 0
JRNL AUTH L.FEHR,L.A.R.CARVALHO,B.H.ROSS,K.LUM,A.C.VIEIRA,
JRNL AUTH 2 R.KIEFERSAUER,R.GEISS-FRIEDLANDER,M.KAISER,T.RODRIGUES,
JRNL AUTH 3 S.D.LUCAS,B.F.CRAVATT,R.HUBER,R.MOREIRA
JRNL TITL DISCOVERY AND DEVELOPMENT OF 4-OXO-BETA-LACTAMS AS NOVEL
JRNL TITL 2 INHIBITORS OF DIPEPTIDYL PEPTIDASES 8 AND 9
JRNL REF TO BE PUBLISHED
JRNL REFN
REMARK 2
REMARK 2 RESOLUTION. 2.80 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : REFMAC 5.8.0155
REMARK 3 AUTHORS : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK 3 : NICHOLLS,WINN,LONG,VAGIN
REMARK 3
REMARK 3 REFINEMENT TARGET : NULL
REMARK 3
REMARK 3 DATA USED IN REFINEMENT.
REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS) : 2.80
REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS) : 49.43
REMARK 3 DATA CUTOFF (SIGMA(F)) : 0.000
REMARK 3 COMPLETENESS FOR RANGE (%) : 100.0
REMARK 3 NUMBER OF REFLECTIONS : 83992
REMARK 3
REMARK 3 FIT TO DATA USED IN REFINEMENT.
REMARK 3 CROSS-VALIDATION METHOD : THROUGHOUT
REMARK 3 FREE R VALUE TEST SET SELECTION : RANDOM
REMARK 3 R VALUE (WORKING + TEST SET) : 0.211
REMARK 3 R VALUE (WORKING SET) : 0.209
REMARK 3 FREE R VALUE : 0.242
REMARK 3 FREE R VALUE TEST SET SIZE (%) : 5.000
REMARK 3 FREE R VALUE TEST SET COUNT : 4421
REMARK 3
REMARK 3 FIT IN THE HIGHEST RESOLUTION BIN.
REMARK 3 TOTAL NUMBER OF BINS USED : 20
REMARK 3 BIN RESOLUTION RANGE HIGH (A) : 2.80
REMARK 3 BIN RESOLUTION RANGE LOW (A) : 2.87
REMARK 3 REFLECTION IN BIN (WORKING SET) : 6151
REMARK 3 BIN COMPLETENESS (WORKING+TEST) (%) : 99.97
REMARK 3 BIN R VALUE (WORKING SET) : 0.3780
REMARK 3 BIN FREE R VALUE SET COUNT : 324
REMARK 3 BIN FREE R VALUE : 0.3520
REMARK 3
REMARK 3 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK 3 PROTEIN ATOMS : 13435
REMARK 3 NUCLEIC ACID ATOMS : 0
REMARK 3 HETEROGEN ATOMS : 206
REMARK 3 SOLVENT ATOMS : 10
REMARK 3
REMARK 3 B VALUES.
REMARK 3 FROM WILSON PLOT (A**2) : 78.12
REMARK 3 MEAN B VALUE (OVERALL, A**2) : 89.57
REMARK 3 OVERALL ANISOTROPIC B VALUE.
REMARK 3 B11 (A**2) : 2.07000
REMARK 3 B22 (A**2) : 2.07000
REMARK 3 B33 (A**2) : -6.70000
REMARK 3 B12 (A**2) : 1.03000
REMARK 3 B13 (A**2) : 0.00000
REMARK 3 B23 (A**2) : 0.00000
REMARK 3
REMARK 3 ESTIMATED OVERALL COORDINATE ERROR.
REMARK 3 ESU BASED ON R VALUE (A): 0.406
REMARK 3 ESU BASED ON FREE R VALUE (A): 0.278
REMARK 3 ESU BASED ON MAXIMUM LIKELIHOOD (A): 0.253
REMARK 3 ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 13.832
REMARK 3
REMARK 3 CORRELATION COEFFICIENTS.
REMARK 3 CORRELATION COEFFICIENT FO-FC : 0.949
REMARK 3 CORRELATION COEFFICIENT FO-FC FREE : 0.930
REMARK 3
REMARK 3 RMS DEVIATIONS FROM IDEAL VALUES COUNT RMS WEIGHT
REMARK 3 BOND LENGTHS REFINED ATOMS (A): 14018 ; 0.007 ; 0.019
REMARK 3 BOND LENGTHS OTHERS (A): 12993 ; 0.002 ; 0.020
REMARK 3 BOND ANGLES REFINED ATOMS (DEGREES): 19038 ; 1.365 ; 1.971
REMARK 3 BOND ANGLES OTHERS (DEGREES): 29960 ; 0.866 ; 3.000
REMARK 3 TORSION ANGLES, PERIOD 1 (DEGREES): 1651 ; 6.085 ; 5.000
REMARK 3 TORSION ANGLES, PERIOD 2 (DEGREES): 673 ;34.629 ;23.536
REMARK 3 TORSION ANGLES, PERIOD 3 (DEGREES): 2309 ;14.126 ;15.000
REMARK 3 TORSION ANGLES, PERIOD 4 (DEGREES): 88 ;13.966 ;15.000
REMARK 3 CHIRAL-CENTER RESTRAINTS (A**3): 2001 ; 0.072 ; 0.200
REMARK 3 GENERAL PLANES REFINED ATOMS (A): 15636 ; 0.004 ; 0.021
REMARK 3 GENERAL PLANES OTHERS (A): 3314 ; 0.001 ; 0.020
REMARK 3 NON-BONDED CONTACTS REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 NON-BONDED CONTACTS OTHERS (A): NULL ; NULL ; NULL
REMARK 3 NON-BONDED TORSION REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 NON-BONDED TORSION OTHERS (A): NULL ; NULL ; NULL
REMARK 3 H-BOND (X...Y) REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 H-BOND (X...Y) OTHERS (A): NULL ; NULL ; NULL
REMARK 3 POTENTIAL METAL-ION REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 POTENTIAL METAL-ION OTHERS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY VDW REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY VDW OTHERS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY H-BOND REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY H-BOND OTHERS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY METAL-ION REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY METAL-ION OTHERS (A): NULL ; NULL ; NULL
REMARK 3
REMARK 3 ISOTROPIC THERMAL FACTOR RESTRAINTS. COUNT RMS WEIGHT
REMARK 3 MAIN-CHAIN BOND REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 MAIN-CHAIN BOND OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 MAIN-CHAIN ANGLE REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 MAIN-CHAIN ANGLE OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SIDE-CHAIN BOND REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SIDE-CHAIN BOND OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SIDE-CHAIN ANGLE REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SIDE-CHAIN ANGLE OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 LONG RANGE B REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 LONG RANGE B OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3
REMARK 3 ANISOTROPIC THERMAL FACTOR RESTRAINTS. COUNT RMS WEIGHT
REMARK 3 RIGID-BOND RESTRAINTS (A**2): NULL ; NULL ; NULL
REMARK 3 SPHERICITY; FREE ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SPHERICITY; BONDED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3
REMARK 3 NCS RESTRAINTS STATISTICS
REMARK 3 NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK 3
REMARK 3 TLS DETAILS
REMARK 3 NUMBER OF TLS GROUPS : NULL
REMARK 3
REMARK 3 BULK SOLVENT MODELLING.
REMARK 3 METHOD USED : NULL
REMARK 3 PARAMETERS FOR MASK CALCULATION
REMARK 3 VDW PROBE RADIUS : 1.20
REMARK 3 ION PROBE RADIUS : 0.80
REMARK 3 SHRINKAGE RADIUS : 0.80
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING
REMARK 3 POSITIONS U VALUES : REFINED INDIVIDUALLY
REMARK 4
REMARK 4 7A3G COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 19-AUG-20.
REMARK 100 THE DEPOSITION ID IS D_1292108109.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200 EXPERIMENT TYPE : X-RAY DIFFRACTION
REMARK 200 DATE OF DATA COLLECTION : 12-FEB-20
REMARK 200 TEMPERATURE (KELVIN) : 100
REMARK 200 PH : NULL
REMARK 200 NUMBER OF CRYSTALS USED : 1
REMARK 200
REMARK 200 SYNCHROTRON (Y/N) : Y
REMARK 200 RADIATION SOURCE : SLS
REMARK 200 BEAMLINE : X10SA
REMARK 200 X-RAY GENERATOR MODEL : NULL
REMARK 200 MONOCHROMATIC OR LAUE (M/L) : M
REMARK 200 WAVELENGTH OR RANGE (A) : 1.0
REMARK 200 MONOCHROMATOR : NULL
REMARK 200 OPTICS : NULL
REMARK 200
REMARK 200 DETECTOR TYPE : PIXEL
REMARK 200 DETECTOR MANUFACTURER : DECTRIS EIGER2 X 16M
REMARK 200 INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200 DATA SCALING SOFTWARE : XSCALE
REMARK 200
REMARK 200 NUMBER OF UNIQUE REFLECTIONS : 88413
REMARK 200 RESOLUTION RANGE HIGH (A) : 2.800
REMARK 200 RESOLUTION RANGE LOW (A) : 49.430
REMARK 200 REJECTION CRITERIA (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200 COMPLETENESS FOR RANGE (%) : 100.0
REMARK 200 DATA REDUNDANCY : 13.17
REMARK 200 R MERGE (I) : 0.09100
REMARK 200 R SYM (I) : NULL
REMARK 200 FOR THE DATA SET : 19.1700
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.80
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 2.87
REMARK 200 COMPLETENESS FOR SHELL (%) : 100.0
REMARK 200 DATA REDUNDANCY IN SHELL : 13.65
REMARK 200 R MERGE FOR SHELL (I) : 1.54600
REMARK 200 R SYM FOR SHELL (I) : NULL
REMARK 200 FOR SHELL : 2.040
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: 6EOP
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS (%): 72.33
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 4.45
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.46 M NA CITRATE, VAPOR DIFFUSION,
REMARK 280 HANGING DROP, TEMPERATURE 273K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 61
REMARK 290
REMARK 290 SYMOP SYMMETRY
REMARK 290 NNNMMM OPERATOR
REMARK 290 1555 X,Y,Z
REMARK 290 2555 -Y,X-Y,Z+1/3
REMARK 290 3555 -X+Y,-X,Z+2/3
REMARK 290 4555 -X,-Y,Z+1/2
REMARK 290 5555 Y,-X+Y,Z+5/6
REMARK 290 6555 X-Y,X,Z+1/6
REMARK 290
REMARK 290 WHERE NNN -> OPERATOR NUMBER
REMARK 290 MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000
REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000
REMARK 290 SMTRY1 2 -0.500000 -0.866025 0.000000 0.00000
REMARK 290 SMTRY2 2 0.866025 -0.500000 0.000000 0.00000
REMARK 290 SMTRY3 2 0.000000 0.000000 1.000000 90.07400
REMARK 290 SMTRY1 3 -0.500000 0.866025 0.000000 0.00000
REMARK 290 SMTRY2 3 -0.866025 -0.500000 0.000000 0.00000
REMARK 290 SMTRY3 3 0.000000 0.000000 1.000000 180.14800
REMARK 290 SMTRY1 4 -1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 4 0.000000 -1.000000 0.000000 0.00000
REMARK 290 SMTRY3 4 0.000000 0.000000 1.000000 135.11100
REMARK 290 SMTRY1 5 0.500000 0.866025 0.000000 0.00000
REMARK 290 SMTRY2 5 -0.866025 0.500000 0.000000 0.00000
REMARK 290 SMTRY3 5 0.000000 0.000000 1.000000 225.18500
REMARK 290 SMTRY1 6 0.500000 -0.866025 0.000000 0.00000
REMARK 290 SMTRY2 6 0.866025 0.500000 0.000000 0.00000
REMARK 290 SMTRY3 6 0.000000 0.000000 1.000000 45.03700
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 7370 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 64740 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -72.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B, A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 MET B 1
REMARK 465 TRP B 2
REMARK 465 LYS B 3
REMARK 465 ARG B 4
REMARK 465 SER B 5
REMARK 465 GLU B 6
REMARK 465 GLN B 7
REMARK 465 MET B 8
REMARK 465 LYS B 9
REMARK 465 ILE B 10
REMARK 465 LYS B 11
REMARK 465 SER B 12
REMARK 465 GLY B 13
REMARK 465 LYS B 14
REMARK 465 CYS B 15
REMARK 465 ASN B 16
REMARK 465 MET B 17
REMARK 465 ALA B 18
REMARK 465 ALA B 19
REMARK 465 ALA B 20
REMARK 465 MET B 21
REMARK 465 GLU B 22
REMARK 465 THR B 23
REMARK 465 GLU B 24
REMARK 465 GLN B 25
REMARK 465 LEU B 26
REMARK 465 GLY B 27
REMARK 465 VAL B 28
REMARK 465 GLU B 29
REMARK 465 ILE B 30
REMARK 465 PHE B 31
REMARK 465 GLU B 32
REMARK 465 THR B 33
REMARK 465 ALA B 34
REMARK 465 ASP B 35
REMARK 465 CYS B 36
REMARK 465 GLU B 37
REMARK 465 GLU B 38
REMARK 465 ASN B 39
REMARK 465 ILE B 40
REMARK 465 GLU B 41
REMARK 465 SER B 42
REMARK 465 GLN B 43
REMARK 465 ASP B 44
REMARK 465 ARG B 45
REMARK 465 GLN B 140
REMARK 465 ALA B 141
REMARK 465 THR B 142
REMARK 465 LEU B 143
REMARK 465 ASP B 144
REMARK 465 TYR B 145
REMARK 465 GLY B 146
REMARK 465 MET B 147
REMARK 465 TYR B 148
REMARK 465 SER B 149
REMARK 465 ARG B 150
REMARK 465 GLU B 151
REMARK 465 GLU B 152
REMARK 465 GLU B 153
REMARK 465 LEU B 154
REMARK 465 LEU B 155
REMARK 465 ARG B 156
REMARK 465 GLU B 157
REMARK 465 ARG B 158
REMARK 465 LYS B 159
REMARK 465 ARG B 160
REMARK 465 ILE B 161
REMARK 465 GLY B 162
REMARK 465 THR B 163
REMARK 465 ILE B 898
REMARK 465 MET A 1
REMARK 465 TRP A 2
REMARK 465 LYS A 3
REMARK 465 ARG A 4
REMARK 465 SER A 5
REMARK 465 GLU A 6
REMARK 465 GLN A 7
REMARK 465 MET A 8
REMARK 465 LYS A 9
REMARK 465 ILE A 10
REMARK 465 LYS A 11
REMARK 465 SER A 12
REMARK 465 GLY A 13
REMARK 465 LYS A 14
REMARK 465 CYS A 15
REMARK 465 ASN A 16
REMARK 465 MET A 17
REMARK 465 ALA A 18
REMARK 465 ALA A 19
REMARK 465 ALA A 20
REMARK 465 MET A 21
REMARK 465 GLU A 22
REMARK 465 THR A 23
REMARK 465 GLU A 24
REMARK 465 GLN A 25
REMARK 465 LEU A 26
REMARK 465 GLY A 27
REMARK 465 VAL A 28
REMARK 465 GLU A 29
REMARK 465 ILE A 30
REMARK 465 PHE A 31
REMARK 465 GLU A 32
REMARK 465 THR A 33
REMARK 465 ALA A 34
REMARK 465 ASP A 35
REMARK 465 CYS A 36
REMARK 465 GLU A 37
REMARK 465 GLU A 38
REMARK 465 ASN A 39
REMARK 465 ILE A 40
REMARK 465 GLU A 41
REMARK 465 SER A 42
REMARK 465 GLN A 43
REMARK 465 ASP A 44
REMARK 465 ARG A 45
REMARK 465 PHE A 139
REMARK 465 GLN A 140
REMARK 465 ALA A 141
REMARK 465 THR A 142
REMARK 465 LEU A 143
REMARK 465 ASP A 144
REMARK 465 TYR A 145
REMARK 465 GLY A 146
REMARK 465 MET A 147
REMARK 465 TYR A 148
REMARK 465 SER A 149
REMARK 465 ARG A 150
REMARK 465 GLU A 151
REMARK 465 GLU A 152
REMARK 465 GLU A 153
REMARK 465 LEU A 154
REMARK 465 LEU A 155
REMARK 465 ARG A 156
REMARK 465 GLU A 157
REMARK 465 ARG A 158
REMARK 465 LYS A 159
REMARK 465 ARG A 160
REMARK 465 ILE A 161
REMARK 465 GLY A 162
REMARK 465 THR A 163
REMARK 465 ILE A 898
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 LEU B 48 157.73 66.52
REMARK 500 GLU B 107 -118.59 59.11
REMARK 500 LEU B 138 -134.83 62.87
REMARK 500 SER B 233 58.38 32.59
REMARK 500 VAL B 242 -62.52 -108.08
REMARK 500 MET B 320 88.27 -68.32
REMARK 500 ILE B 445 -85.98 -100.46
REMARK 500 PHE B 453 110.04 -177.36
REMARK 500 LEU B 486 64.69 -103.42
REMARK 500 GLU B 511 74.79 -155.02
REMARK 500 GLU B 518 57.36 -95.25
REMARK 500 PRO B 560 104.25 -59.52
REMARK 500 PRO B 594 177.16 -57.64
REMARK 500 ASP B 629 -19.98 78.82
REMARK 500 TYR B 669 -70.98 -120.25
REMARK 500 VAL B 684 -73.33 -94.02
REMARK 500 TYR B 686 40.51 -101.57
REMARK 500 TYR B 720 6.25 59.83
REMARK 500 SER B 755 -107.62 67.97
REMARK 500 ARG B 768 59.68 -113.63
REMARK 500 LEU B 783 96.66 -163.20
REMARK 500 ASN B 802 58.43 -157.93
REMARK 500 GLN B 862 44.31 -109.32
REMARK 500 ARG B 864 -138.43 -97.68
REMARK 500 LEU B 888 -63.47 -133.60
REMARK 500 LYS A 47 -73.38 -132.44
REMARK 500 LEU A 48 142.12 61.68
REMARK 500 MET A 76 63.78 -114.32
REMARK 500 GLU A 107 -94.21 58.63
REMARK 500 SER A 233 54.12 39.21
REMARK 500 VAL A 242 -61.72 -108.67
REMARK 500 ILE A 445 -87.56 -102.15
REMARK 500 PHE A 453 110.77 -178.42
REMARK 500 LEU A 486 73.45 -104.89
REMARK 500 GLU A 511 81.99 -153.61
REMARK 500 GLU A 518 58.99 -95.46
REMARK 500 ASN A 559 78.17 -116.67
REMARK 500 PRO A 594 176.75 -58.86
REMARK 500 ASP A 629 -11.44 93.60
REMARK 500 ASP A 655 71.44 52.44
REMARK 500 TYR A 669 -71.03 -118.13
REMARK 500 TYR A 686 43.72 -104.83
REMARK 500 TYR A 720 2.82 60.00
REMARK 500 SER A 755 -108.14 65.90
REMARK 500 ALA A 779 72.98 -155.25
REMARK 500 LEU A 783 96.92 -163.30
REMARK 500 ASN A 802 60.30 -159.22
REMARK 500 SER A 820 31.12 -96.38
REMARK 500 GLN A 862 44.49 -109.76
REMARK 500 ARG A 864 -132.31 -95.63
REMARK 500
REMARK 500 THIS ENTRY HAS 51 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 6EOP RELATED DB: PDB
REMARK 900 DPP8 IN COMPLEX WITH AN INHIBITORY PEPTIDE
DBREF 7A3G B 1 898 UNP Q6V1X1 DPP8_HUMAN 1 898
DBREF 7A3G A 1 898 UNP Q6V1X1 DPP8_HUMAN 1 898
SEQRES 1 B 898 MET TRP LYS ARG SER GLU GLN MET LYS ILE LYS SER GLY
SEQRES 2 B 898 LYS CYS ASN MET ALA ALA ALA MET GLU THR GLU GLN LEU
SEQRES 3 B 898 GLY VAL GLU ILE PHE GLU THR ALA ASP CYS GLU GLU ASN
SEQRES 4 B 898 ILE GLU SER GLN ASP ARG PRO LYS LEU GLU PRO PHE TYR
SEQRES 5 B 898 VAL GLU ARG TYR SER TRP SER GLN LEU LYS LYS LEU LEU
SEQRES 6 B 898 ALA ASP THR ARG LYS TYR HIS GLY TYR MET MET ALA LYS
SEQRES 7 B 898 ALA PRO HIS ASP PHE MET PHE VAL LYS ARG ASN ASP PRO
SEQRES 8 B 898 ASP GLY PRO HIS SER ASP ARG ILE TYR TYR LEU ALA MET
SEQRES 9 B 898 SER GLY GLU ASN ARG GLU ASN THR LEU PHE TYR SER GLU
SEQRES 10 B 898 ILE PRO LYS THR ILE ASN ARG ALA ALA VAL LEU MET LEU
SEQRES 11 B 898 SER TRP LYS PRO LEU LEU ASP LEU PHE GLN ALA THR LEU
SEQRES 12 B 898 ASP TYR GLY MET TYR SER ARG GLU GLU GLU LEU LEU ARG
SEQRES 13 B 898 GLU ARG LYS ARG ILE GLY THR VAL GLY ILE ALA SER TYR
SEQRES 14 B 898 ASP TYR HIS GLN GLY SER GLY THR PHE LEU PHE GLN ALA
SEQRES 15 B 898 GLY SER GLY ILE TYR HIS VAL LYS ASP GLY GLY PRO GLN
SEQRES 16 B 898 GLY PHE THR GLN GLN PRO LEU ARG PRO ASN LEU VAL GLU
SEQRES 17 B 898 THR SER CYS PRO ASN ILE ARG MET ASP PRO LYS LEU CYS
SEQRES 18 B 898 PRO ALA ASP PRO ASP TRP ILE ALA PHE ILE HIS SER ASN
SEQRES 19 B 898 ASP ILE TRP ILE SER ASN ILE VAL THR ARG GLU GLU ARG
SEQRES 20 B 898 ARG LEU THR TYR VAL HIS ASN GLU LEU ALA ASN MET GLU
SEQRES 21 B 898 GLU ASP ALA ARG SER ALA GLY VAL ALA THR PHE VAL LEU
SEQRES 22 B 898 GLN GLU GLU PHE ASP ARG TYR SER GLY TYR TRP TRP CYS
SEQRES 23 B 898 PRO LYS ALA GLU THR THR PRO SER GLY GLY LYS ILE LEU
SEQRES 24 B 898 ARG ILE LEU TYR GLU GLU ASN ASP GLU SER GLU VAL GLU
SEQRES 25 B 898 ILE ILE HIS VAL THR SER PRO MET LEU GLU THR ARG ARG
SEQRES 26 B 898 ALA ASP SER PHE ARG TYR PRO LYS THR GLY THR ALA ASN
SEQRES 27 B 898 PRO LYS VAL THR PHE LYS MET SER GLU ILE MET ILE ASP
SEQRES 28 B 898 ALA GLU GLY ARG ILE ILE ASP VAL ILE ASP LYS GLU LEU
SEQRES 29 B 898 ILE GLN PRO PHE GLU ILE LEU PHE GLU GLY VAL GLU TYR
SEQRES 30 B 898 ILE ALA ARG ALA GLY TRP THR PRO GLU GLY LYS TYR ALA
SEQRES 31 B 898 TRP SER ILE LEU LEU ASP ARG SER GLN THR ARG LEU GLN
SEQRES 32 B 898 ILE VAL LEU ILE SER PRO GLU LEU PHE ILE PRO VAL GLU
SEQRES 33 B 898 ASP ASP VAL MET GLU ARG GLN ARG LEU ILE GLU SER VAL
SEQRES 34 B 898 PRO ASP SER VAL THR PRO LEU ILE ILE TYR GLU GLU THR
SEQRES 35 B 898 THR ASP ILE TRP ILE ASN ILE HIS ASP ILE PHE HIS VAL
SEQRES 36 B 898 PHE PRO GLN SER HIS GLU GLU GLU ILE GLU PHE ILE PHE
SEQRES 37 B 898 ALA SER GLU CYS LYS THR GLY PHE ARG HIS LEU TYR LYS
SEQRES 38 B 898 ILE THR SER ILE LEU LYS GLU SER LYS TYR LYS ARG SER
SEQRES 39 B 898 SER GLY GLY LEU PRO ALA PRO SER ASP PHE LYS CYS PRO
SEQRES 40 B 898 ILE LYS GLU GLU ILE ALA ILE THR SER GLY GLU TRP GLU
SEQRES 41 B 898 VAL LEU GLY ARG HIS GLY SER ASN ILE GLN VAL ASP GLU
SEQRES 42 B 898 VAL ARG ARG LEU VAL TYR PHE GLU GLY THR LYS ASP SER
SEQRES 43 B 898 PRO LEU GLU HIS HIS LEU TYR VAL VAL SER TYR VAL ASN
SEQRES 44 B 898 PRO GLY GLU VAL THR ARG LEU THR ASP ARG GLY TYR SER
SEQRES 45 B 898 HIS SER CYS CYS ILE SER GLN HIS CYS ASP PHE PHE ILE
SEQRES 46 B 898 SER LYS TYR SER ASN GLN LYS ASN PRO HIS CYS VAL SER
SEQRES 47 B 898 LEU TYR LYS LEU SER SER PRO GLU ASP ASP PRO THR CYS
SEQRES 48 B 898 LYS THR LYS GLU PHE TRP ALA THR ILE LEU ASP SER ALA
SEQRES 49 B 898 GLY PRO LEU PRO ASP TYR THR PRO PRO GLU ILE PHE SER
SEQRES 50 B 898 PHE GLU SER THR THR GLY PHE THR LEU TYR GLY MET LEU
SEQRES 51 B 898 TYR LYS PRO HIS ASP LEU GLN PRO GLY LYS LYS TYR PRO
SEQRES 52 B 898 THR VAL LEU PHE ILE TYR GLY GLY PRO GLN VAL GLN LEU
SEQRES 53 B 898 VAL ASN ASN ARG PHE LYS GLY VAL LYS TYR PHE ARG LEU
SEQRES 54 B 898 ASN THR LEU ALA SER LEU GLY TYR VAL VAL VAL VAL ILE
SEQRES 55 B 898 ASP ASN ARG GLY SER CYS HIS ARG GLY LEU LYS PHE GLU
SEQRES 56 B 898 GLY ALA PHE LYS TYR LYS MET GLY GLN ILE GLU ILE ASP
SEQRES 57 B 898 ASP GLN VAL GLU GLY LEU GLN TYR LEU ALA SER ARG TYR
SEQRES 58 B 898 ASP PHE ILE ASP LEU ASP ARG VAL GLY ILE HIS GLY TRP
SEQRES 59 B 898 SER TYR GLY GLY TYR LEU SER LEU MET ALA LEU MET GLN
SEQRES 60 B 898 ARG SER ASP ILE PHE ARG VAL ALA ILE ALA GLY ALA PRO
SEQRES 61 B 898 VAL THR LEU TRP ILE PHE TYR ASP THR GLY TYR THR GLU
SEQRES 62 B 898 ARG TYR MET GLY HIS PRO ASP GLN ASN GLU GLN GLY TYR
SEQRES 63 B 898 TYR LEU GLY SER VAL ALA MET GLN ALA GLU LYS PHE PRO
SEQRES 64 B 898 SER GLU PRO ASN ARG LEU LEU LEU LEU HIS GLY PHE LEU
SEQRES 65 B 898 ASP GLU ASN VAL HIS PHE ALA HIS THR SER ILE LEU LEU
SEQRES 66 B 898 SER PHE LEU VAL ARG ALA GLY LYS PRO TYR ASP LEU GLN
SEQRES 67 B 898 ILE TYR PRO GLN GLU ARG HIS SER ILE ARG VAL PRO GLU
SEQRES 68 B 898 SER GLY GLU HIS TYR GLU LEU HIS LEU LEU HIS TYR LEU
SEQRES 69 B 898 GLN GLU ASN LEU GLY SER ARG ILE ALA ALA LEU LYS VAL
SEQRES 70 B 898 ILE
SEQRES 1 A 898 MET TRP LYS ARG SER GLU GLN MET LYS ILE LYS SER GLY
SEQRES 2 A 898 LYS CYS ASN MET ALA ALA ALA MET GLU THR GLU GLN LEU
SEQRES 3 A 898 GLY VAL GLU ILE PHE GLU THR ALA ASP CYS GLU GLU ASN
SEQRES 4 A 898 ILE GLU SER GLN ASP ARG PRO LYS LEU GLU PRO PHE TYR
SEQRES 5 A 898 VAL GLU ARG TYR SER TRP SER GLN LEU LYS LYS LEU LEU
SEQRES 6 A 898 ALA ASP THR ARG LYS TYR HIS GLY TYR MET MET ALA LYS
SEQRES 7 A 898 ALA PRO HIS ASP PHE MET PHE VAL LYS ARG ASN ASP PRO
SEQRES 8 A 898 ASP GLY PRO HIS SER ASP ARG ILE TYR TYR LEU ALA MET
SEQRES 9 A 898 SER GLY GLU ASN ARG GLU ASN THR LEU PHE TYR SER GLU
SEQRES 10 A 898 ILE PRO LYS THR ILE ASN ARG ALA ALA VAL LEU MET LEU
SEQRES 11 A 898 SER TRP LYS PRO LEU LEU ASP LEU PHE GLN ALA THR LEU
SEQRES 12 A 898 ASP TYR GLY MET TYR SER ARG GLU GLU GLU LEU LEU ARG
SEQRES 13 A 898 GLU ARG LYS ARG ILE GLY THR VAL GLY ILE ALA SER TYR
SEQRES 14 A 898 ASP TYR HIS GLN GLY SER GLY THR PHE LEU PHE GLN ALA
SEQRES 15 A 898 GLY SER GLY ILE TYR HIS VAL LYS ASP GLY GLY PRO GLN
SEQRES 16 A 898 GLY PHE THR GLN GLN PRO LEU ARG PRO ASN LEU VAL GLU
SEQRES 17 A 898 THR SER CYS PRO ASN ILE ARG MET ASP PRO LYS LEU CYS
SEQRES 18 A 898 PRO ALA ASP PRO ASP TRP ILE ALA PHE ILE HIS SER ASN
SEQRES 19 A 898 ASP ILE TRP ILE SER ASN ILE VAL THR ARG GLU GLU ARG
SEQRES 20 A 898 ARG LEU THR TYR VAL HIS ASN GLU LEU ALA ASN MET GLU
SEQRES 21 A 898 GLU ASP ALA ARG SER ALA GLY VAL ALA THR PHE VAL LEU
SEQRES 22 A 898 GLN GLU GLU PHE ASP ARG TYR SER GLY TYR TRP TRP CYS
SEQRES 23 A 898 PRO LYS ALA GLU THR THR PRO SER GLY GLY LYS ILE LEU
SEQRES 24 A 898 ARG ILE LEU TYR GLU GLU ASN ASP GLU SER GLU VAL GLU
SEQRES 25 A 898 ILE ILE HIS VAL THR SER PRO MET LEU GLU THR ARG ARG
SEQRES 26 A 898 ALA ASP SER PHE ARG TYR PRO LYS THR GLY THR ALA ASN
SEQRES 27 A 898 PRO LYS VAL THR PHE LYS MET SER GLU ILE MET ILE ASP
SEQRES 28 A 898 ALA GLU GLY ARG ILE ILE ASP VAL ILE ASP LYS GLU LEU
SEQRES 29 A 898 ILE GLN PRO PHE GLU ILE LEU PHE GLU GLY VAL GLU TYR
SEQRES 30 A 898 ILE ALA ARG ALA GLY TRP THR PRO GLU GLY LYS TYR ALA
SEQRES 31 A 898 TRP SER ILE LEU LEU ASP ARG SER GLN THR ARG LEU GLN
SEQRES 32 A 898 ILE VAL LEU ILE SER PRO GLU LEU PHE ILE PRO VAL GLU
SEQRES 33 A 898 ASP ASP VAL MET GLU ARG GLN ARG LEU ILE GLU SER VAL
SEQRES 34 A 898 PRO ASP SER VAL THR PRO LEU ILE ILE TYR GLU GLU THR
SEQRES 35 A 898 THR ASP ILE TRP ILE ASN ILE HIS ASP ILE PHE HIS VAL
SEQRES 36 A 898 PHE PRO GLN SER HIS GLU GLU GLU ILE GLU PHE ILE PHE
SEQRES 37 A 898 ALA SER GLU CYS LYS THR GLY PHE ARG HIS LEU TYR LYS
SEQRES 38 A 898 ILE THR SER ILE LEU LYS GLU SER LYS TYR LYS ARG SER
SEQRES 39 A 898 SER GLY GLY LEU PRO ALA PRO SER ASP PHE LYS CYS PRO
SEQRES 40 A 898 ILE LYS GLU GLU ILE ALA ILE THR SER GLY GLU TRP GLU
SEQRES 41 A 898 VAL LEU GLY ARG HIS GLY SER ASN ILE GLN VAL ASP GLU
SEQRES 42 A 898 VAL ARG ARG LEU VAL TYR PHE GLU GLY THR LYS ASP SER
SEQRES 43 A 898 PRO LEU GLU HIS HIS LEU TYR VAL VAL SER TYR VAL ASN
SEQRES 44 A 898 PRO GLY GLU VAL THR ARG LEU THR ASP ARG GLY TYR SER
SEQRES 45 A 898 HIS SER CYS CYS ILE SER GLN HIS CYS ASP PHE PHE ILE
SEQRES 46 A 898 SER LYS TYR SER ASN GLN LYS ASN PRO HIS CYS VAL SER
SEQRES 47 A 898 LEU TYR LYS LEU SER SER PRO GLU ASP ASP PRO THR CYS
SEQRES 48 A 898 LYS THR LYS GLU PHE TRP ALA THR ILE LEU ASP SER ALA
SEQRES 49 A 898 GLY PRO LEU PRO ASP TYR THR PRO PRO GLU ILE PHE SER
SEQRES 50 A 898 PHE GLU SER THR THR GLY PHE THR LEU TYR GLY MET LEU
SEQRES 51 A 898 TYR LYS PRO HIS ASP LEU GLN PRO GLY LYS LYS TYR PRO
SEQRES 52 A 898 THR VAL LEU PHE ILE TYR GLY GLY PRO GLN VAL GLN LEU
SEQRES 53 A 898 VAL ASN ASN ARG PHE LYS GLY VAL LYS TYR PHE ARG LEU
SEQRES 54 A 898 ASN THR LEU ALA SER LEU GLY TYR VAL VAL VAL VAL ILE
SEQRES 55 A 898 ASP ASN ARG GLY SER CYS HIS ARG GLY LEU LYS PHE GLU
SEQRES 56 A 898 GLY ALA PHE LYS TYR LYS MET GLY GLN ILE GLU ILE ASP
SEQRES 57 A 898 ASP GLN VAL GLU GLY LEU GLN TYR LEU ALA SER ARG TYR
SEQRES 58 A 898 ASP PHE ILE ASP LEU ASP ARG VAL GLY ILE HIS GLY TRP
SEQRES 59 A 898 SER TYR GLY GLY TYR LEU SER LEU MET ALA LEU MET GLN
SEQRES 60 A 898 ARG SER ASP ILE PHE ARG VAL ALA ILE ALA GLY ALA PRO
SEQRES 61 A 898 VAL THR LEU TRP ILE PHE TYR ASP THR GLY TYR THR GLU
SEQRES 62 A 898 ARG TYR MET GLY HIS PRO ASP GLN ASN GLU GLN GLY TYR
SEQRES 63 A 898 TYR LEU GLY SER VAL ALA MET GLN ALA GLU LYS PHE PRO
SEQRES 64 A 898 SER GLU PRO ASN ARG LEU LEU LEU LEU HIS GLY PHE LEU
SEQRES 65 A 898 ASP GLU ASN VAL HIS PHE ALA HIS THR SER ILE LEU LEU
SEQRES 66 A 898 SER PHE LEU VAL ARG ALA GLY LYS PRO TYR ASP LEU GLN
SEQRES 67 A 898 ILE TYR PRO GLN GLU ARG HIS SER ILE ARG VAL PRO GLU
SEQRES 68 A 898 SER GLY GLU HIS TYR GLU LEU HIS LEU LEU HIS TYR LEU
SEQRES 69 A 898 GLN GLU ASN LEU GLY SER ARG ILE ALA ALA LEU LYS VAL
SEQRES 70 A 898 ILE
HET TMO B 901 5
HET QXQ B 902 30
HET TMO B 903 5
HET GOL B 904 6
HET GOL B 905 6
HET PO4 B 906 5
HET PO4 B 907 5
HET PO4 B 908 5
HET PO4 B 909 5
HET QXT B 910 30
HET CL B 911 1
HET TMO A1001 5
HET QXQ A1002 30
HET TMO A1003 5
HET GOL A1004 6
HET GOL A1005 6
HET PO4 A1006 5
HET PO4 A1007 5
HET PO4 A1008 5
HET PO4 A1009 5
HET QXT A1010 30
HET CL A1011 1
HETNAM TMO TRIMETHYLAMINE OXIDE
HETNAM QXQ ~{N}-[3-(7,8-DIHYDRO-5~{H}-[1,3]DIOXOLO[4,5-
HETNAM 2 QXQ G]ISOQUINOLIN-6-YLMETHYL)PHENYL]-2-ETHYL-2-METHANOYL-
HETNAM 3 QXQ BUTANAMIDE
HETNAM GOL GLYCEROL
HETNAM PO4 PHOSPHATE ION
HETNAM QXT 1-[3-(7,8-DIHYDRO-5~{H}-[1,3]DIOXOLO[4,5-G]ISOQUINOLIN-
HETNAM 2 QXT 6-YLMETHYL)PHENYL]-3,3-DIETHYL-AZETIDINE-2,4-DIONE
HETNAM CL CHLORIDE ION
HETSYN GOL GLYCERIN; PROPANE-1,2,3-TRIOL
FORMUL 3 TMO 4(C3 H9 N O)
FORMUL 4 QXQ 2(C24 H28 N2 O4)
FORMUL 6 GOL 4(C3 H8 O3)
FORMUL 8 PO4 8(O4 P 3-)
FORMUL 12 QXT 2(C24 H26 N2 O4)
FORMUL 13 CL 2(CL 1-)
FORMUL 25 HOH *10(H2 O)
HELIX 1 AA1 SER B 57 HIS B 72 1 16
HELIX 2 AA2 THR B 270 ASP B 278 1 9
HELIX 3 AA3 MET B 320 ARG B 324 5 5
HELIX 4 AA4 PRO B 367 PHE B 372 1 6
HELIX 5 AA5 SER B 408 GLU B 410 5 3
HELIX 6 AA6 ASP B 418 VAL B 429 1 12
HELIX 7 AA7 LYS B 492 GLY B 496 5 5
HELIX 8 AA8 TYR B 686 LEU B 695 1 10
HELIX 9 AA9 ARG B 710 ALA B 717 1 8
HELIX 10 AB1 ILE B 725 TYR B 741 1 17
HELIX 11 AB2 SER B 755 ARG B 768 1 14
HELIX 12 AB3 LEU B 783 TYR B 787 5 5
HELIX 13 AB4 ASP B 788 GLY B 797 1 10
HELIX 14 AB5 HIS B 798 GLN B 801 5 4
HELIX 15 AB6 ASN B 802 GLY B 809 1 8
HELIX 16 AB7 SER B 810 PHE B 818 5 9
HELIX 17 AB8 PHE B 838 ALA B 851 1 14
HELIX 18 AB9 VAL B 869 LEU B 888 1 20
HELIX 19 AC1 SER B 890 VAL B 897 1 8
HELIX 20 AC2 SER A 57 HIS A 72 1 16
HELIX 21 AC3 THR A 270 ASP A 278 1 9
HELIX 22 AC4 MET A 320 ARG A 324 5 5
HELIX 23 AC5 PRO A 367 PHE A 372 1 6
HELIX 24 AC6 SER A 408 GLU A 410 5 3
HELIX 25 AC7 ASP A 418 VAL A 429 1 12
HELIX 26 AC8 LYS A 492 GLY A 496 5 5
HELIX 27 AC9 TYR A 686 GLY A 696 1 11
HELIX 28 AD1 GLY A 711 GLY A 716 1 6
HELIX 29 AD2 ALA A 717 LYS A 719 5 3
HELIX 30 AD3 ILE A 725 TYR A 741 1 17
HELIX 31 AD4 SER A 755 ARG A 768 1 14
HELIX 32 AD5 LEU A 783 TYR A 787 5 5
HELIX 33 AD6 ASP A 788 GLY A 797 1 10
HELIX 34 AD7 HIS A 798 GLN A 801 5 4
HELIX 35 AD8 ASN A 802 GLY A 809 1 8
HELIX 36 AD9 SER A 810 PHE A 818 5 9
HELIX 37 AE1 PHE A 838 ALA A 851 1 14
HELIX 38 AE2 VAL A 869 LEU A 888 1 20
HELIX 39 AE3 SER A 890 VAL A 897 1 8
SHEET 1 AA1 5 GLU B 49 PRO B 50 0
SHEET 2 AA1 5 LYS B 660 PHE B 667 1 O LYS B 661 N GLU B 49
SHEET 3 AA1 5 VAL B 698 ILE B 702 1 O VAL B 698 N VAL B 665
SHEET 4 AA1 5 THR B 645 TYR B 651 -1 N TYR B 651 O VAL B 699
SHEET 5 AA1 5 GLU B 634 GLU B 639 -1 N GLU B 634 O LEU B 650
SHEET 1 AA2 6 GLU B 49 PRO B 50 0
SHEET 2 AA2 6 LYS B 660 PHE B 667 1 O LYS B 661 N GLU B 49
SHEET 3 AA2 6 ILE B 744 TRP B 754 1 O GLY B 750 N LEU B 666
SHEET 4 AA2 6 PHE B 772 GLY B 778 1 O ILE B 776 N ILE B 751
SHEET 5 AA2 6 LEU B 825 GLY B 830 1 O LEU B 828 N ALA B 777
SHEET 6 AA2 6 ASP B 856 TYR B 860 1 O GLN B 858 N LEU B 827
SHEET 1 AA3 4 HIS B 81 LYS B 87 0
SHEET 2 AA3 4 HIS B 95 MET B 104 -1 O ARG B 98 N VAL B 86
SHEET 3 AA3 4 ASN B 111 PRO B 119 -1 O PHE B 114 N TYR B 101
SHEET 4 AA3 4 LYS B 133 PRO B 134 -1 O LYS B 133 N TYR B 115
SHEET 1 AA4 4 ASP B 170 TYR B 171 0
SHEET 2 AA4 4 THR B 177 ALA B 182 -1 O LEU B 179 N ASP B 170
SHEET 3 AA4 4 GLY B 185 LYS B 190 -1 O VAL B 189 N PHE B 178
SHEET 4 AA4 4 ASN B 205 LEU B 206 -1 O ASN B 205 N HIS B 188
SHEET 1 AA5 4 ARG B 215 LEU B 220 0
SHEET 2 AA5 4 TRP B 227 HIS B 232 -1 O ILE B 231 N MET B 216
SHEET 3 AA5 4 ASP B 235 ASN B 240 -1 O TRP B 237 N PHE B 230
SHEET 4 AA5 4 GLU B 246 ARG B 248 -1 O ARG B 247 N ILE B 238
SHEET 1 AA6 3 ARG B 264 ALA B 266 0
SHEET 2 AA6 3 LYS B 297 ASP B 307 -1 O ASN B 306 N SER B 265
SHEET 3 AA6 3 GLU B 290 THR B 291 -1 N GLU B 290 O ILE B 298
SHEET 1 AA7 5 TYR B 283 TRP B 285 0
SHEET 2 AA7 5 LYS B 297 ASP B 307 -1 O LEU B 302 N TRP B 284
SHEET 3 AA7 5 LYS B 340 ILE B 350 -1 O ILE B 350 N LYS B 297
SHEET 4 AA7 5 ILE B 356 LEU B 364 -1 O ILE B 357 N MET B 349
SHEET 5 AA7 5 PHE B 412 PRO B 414 -1 O ILE B 413 N GLU B 363
SHEET 1 AA8 2 ILE B 313 THR B 317 0
SHEET 2 AA8 2 ALA B 326 ARG B 330 -1 O ASP B 327 N VAL B 316
SHEET 1 AA9 4 VAL B 375 TRP B 383 0
SHEET 2 AA9 4 ALA B 390 ASP B 396 -1 O ILE B 393 N ARG B 380
SHEET 3 AA9 4 ARG B 401 ILE B 407 -1 O ILE B 407 N ALA B 390
SHEET 4 AA9 4 LEU B 436 THR B 442 -1 O LEU B 436 N LEU B 406
SHEET 1 AB1 4 PHE B 453 VAL B 455 0
SHEET 2 AB1 4 GLU B 463 SER B 470 -1 O ILE B 467 N HIS B 454
SHEET 3 AB1 4 HIS B 478 ILE B 485 -1 O SER B 484 N ILE B 464
SHEET 4 AB1 4 ILE B 508 ALA B 513 -1 O ILE B 512 N LYS B 481
SHEET 1 AB2 4 ILE B 529 ASP B 532 0
SHEET 2 AB2 4 LEU B 537 SER B 546 -1 O TYR B 539 N GLN B 530
SHEET 3 AB2 4 GLU B 549 SER B 556 -1 O TYR B 553 N PHE B 540
SHEET 4 AB2 4 THR B 564 ARG B 565 -1 O THR B 564 N VAL B 554
SHEET 1 AB3 4 SER B 572 ILE B 577 0
SHEET 2 AB3 4 PHE B 583 SER B 589 -1 O LYS B 587 N SER B 574
SHEET 3 AB3 4 CYS B 596 SER B 603 -1 O CYS B 596 N TYR B 588
SHEET 4 AB3 4 THR B 613 LEU B 621 -1 O ALA B 618 N LEU B 599
SHEET 1 AB4 5 GLU A 49 PRO A 50 0
SHEET 2 AB4 5 LYS A 660 ILE A 668 1 O LYS A 661 N GLU A 49
SHEET 3 AB4 5 VAL A 698 ILE A 702 1 O VAL A 698 N VAL A 665
SHEET 4 AB4 5 THR A 645 TYR A 651 -1 N MET A 649 O VAL A 701
SHEET 5 AB4 5 GLU A 634 GLU A 639 -1 N GLU A 634 O LEU A 650
SHEET 1 AB5 6 GLU A 49 PRO A 50 0
SHEET 2 AB5 6 LYS A 660 ILE A 668 1 O LYS A 661 N GLU A 49
SHEET 3 AB5 6 ILE A 744 GLY A 753 1 O GLY A 750 N LEU A 666
SHEET 4 AB5 6 PHE A 772 ALA A 777 1 O ILE A 776 N ILE A 751
SHEET 5 AB5 6 LEU A 825 GLY A 830 1 O LEU A 828 N ALA A 777
SHEET 6 AB5 6 ASP A 856 TYR A 860 1 O ASP A 856 N LEU A 827
SHEET 1 AB6 4 HIS A 81 LYS A 87 0
SHEET 2 AB6 4 HIS A 95 MET A 104 -1 O ARG A 98 N VAL A 86
SHEET 3 AB6 4 ASN A 111 PRO A 119 -1 O PHE A 114 N TYR A 101
SHEET 4 AB6 4 LYS A 133 PRO A 134 -1 O LYS A 133 N TYR A 115
SHEET 1 AB7 4 ASP A 170 TYR A 171 0
SHEET 2 AB7 4 THR A 177 ALA A 182 -1 O LEU A 179 N ASP A 170
SHEET 3 AB7 4 GLY A 185 LYS A 190 -1 O VAL A 189 N PHE A 178
SHEET 4 AB7 4 ASN A 205 LEU A 206 -1 O ASN A 205 N HIS A 188
SHEET 1 AB8 4 ARG A 215 CYS A 221 0
SHEET 2 AB8 4 ASP A 224 HIS A 232 -1 O ILE A 231 N MET A 216
SHEET 3 AB8 4 ASP A 235 ASN A 240 -1 O TRP A 237 N PHE A 230
SHEET 4 AB8 4 GLU A 246 ARG A 248 -1 O ARG A 247 N ILE A 238
SHEET 1 AB9 3 ARG A 264 ALA A 266 0
SHEET 2 AB9 3 LYS A 297 ASP A 307 -1 O ASN A 306 N SER A 265
SHEET 3 AB9 3 GLU A 290 THR A 291 -1 N GLU A 290 O ILE A 298
SHEET 1 AC1 5 TYR A 283 TRP A 285 0
SHEET 2 AC1 5 LYS A 297 ASP A 307 -1 O LEU A 302 N TRP A 284
SHEET 3 AC1 5 LYS A 340 ILE A 350 -1 O ILE A 350 N LYS A 297
SHEET 4 AC1 5 ILE A 356 LEU A 364 -1 O ILE A 357 N MET A 349
SHEET 5 AC1 5 PHE A 412 PRO A 414 -1 O ILE A 413 N GLU A 363
SHEET 1 AC2 2 ILE A 313 THR A 317 0
SHEET 2 AC2 2 ALA A 326 ARG A 330 -1 O ASP A 327 N VAL A 316
SHEET 1 AC3 4 VAL A 375 TRP A 383 0
SHEET 2 AC3 4 ALA A 390 ASP A 396 -1 O ILE A 393 N ARG A 380
SHEET 3 AC3 4 ARG A 401 ILE A 407 -1 O GLN A 403 N LEU A 394
SHEET 4 AC3 4 LEU A 436 THR A 442 -1 O LEU A 436 N LEU A 406
SHEET 1 AC4 4 HIS A 454 VAL A 455 0
SHEET 2 AC4 4 GLU A 463 SER A 470 -1 O ILE A 467 N HIS A 454
SHEET 3 AC4 4 HIS A 478 ILE A 485 -1 O ILE A 482 N PHE A 466
SHEET 4 AC4 4 ILE A 508 ALA A 513 -1 O ILE A 512 N LYS A 481
SHEET 1 AC5 4 ILE A 529 ASP A 532 0
SHEET 2 AC5 4 LEU A 537 SER A 546 -1 O TYR A 539 N GLN A 530
SHEET 3 AC5 4 GLU A 549 SER A 556 -1 O TYR A 553 N PHE A 540
SHEET 4 AC5 4 THR A 564 ARG A 565 -1 O THR A 564 N VAL A 554
SHEET 1 AC6 4 SER A 572 ILE A 577 0
SHEET 2 AC6 4 PHE A 583 SER A 589 -1 O LYS A 587 N SER A 574
SHEET 3 AC6 4 CYS A 596 SER A 603 -1 O CYS A 596 N TYR A 588
SHEET 4 AC6 4 THR A 613 LEU A 621 -1 O ALA A 618 N LEU A 599
LINK OG SER B 755 C21 QXQ B 902 1555 1555 1.45
LINK OG SER A 755 C21 QXQ A1002 1555 1555 1.45
CRYST1 153.598 153.598 270.222 90.00 90.00 120.00 P 61 12
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 0.006511 0.003759 0.000000 0.00000
SCALE2 0.000000 0.007518 0.000000 0.00000
SCALE3 0.000000 0.000000 0.003701 0.00000
TER 6724 VAL B 897
TER 13437 VAL A 897
MASTER 468 0 22 39 98 0 0 613651 2 206 140
END |