longtext: 7e0n-pdb

content
HEADER    HYDROLASE                               28-JAN-21   7E0N
TITLE     CRYSTAL STRUCTURE OF MONOACYLGLYCEROL LIPASE CHIMERA
CAVEAT     7E0N    THR A 251 HAS WRONG CHIRALITY AT ATOM CB
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: THERMOSTABLE MONOACYLGLYCEROL LIPASE,LIPASE;
COMPND   3 CHAIN: A, B, C, D;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: GEOBACILLUS SP. 12AMOR1, BACILLUS
SOURCE   3 OCEANISEDIMINIS 2691;
SOURCE   4 ORGANISM_TAXID: 1629723, 1196031;
SOURCE   5 GENE: A361_02165;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    MONOACYLGLYCEROL LIPASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    D.M.LAN
REVDAT   1   16-MAR-22 7E0N    0
JRNL        AUTH   D.M.LAN,Y.H.WANG
JRNL        TITL   CRYSTAL STRUCTURE OF MONOACYLGLYCEROL LIPASE CHIMERA
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.85 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.18.1_3865
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : GEOSTD + MONOMER LIBRARY + CDL V1.2
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.85
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 23.63
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350
REMARK   3   COMPLETENESS FOR RANGE        (%) : 93.7
REMARK   3   NUMBER OF REFLECTIONS             : 107963
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.155
REMARK   3   R VALUE            (WORKING SET) : 0.155
REMARK   3   FREE R VALUE                     : 0.178
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 1.760
REMARK   3   FREE R VALUE TEST SET COUNT      : 1904
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 23.6300 -  4.4500    0.83     7103   126  0.1538 0.1559
REMARK   3     2  4.4500 -  3.5400    0.98     8030   145  0.1322 0.1549
REMARK   3     3  3.5400 -  3.0900    0.99     8026   143  0.1476 0.1806
REMARK   3     4  3.0900 -  2.8100    0.98     7953   144  0.1530 0.1799
REMARK   3     5  2.8100 -  2.6100    0.98     7929   142  0.1541 0.1795
REMARK   3     6  2.6100 -  2.4500    0.97     7871   140  0.1570 0.1658
REMARK   3     7  2.4500 -  2.3300    0.97     7758   139  0.1580 0.1952
REMARK   3     8  2.3300 -  2.2300    0.97     7794   141  0.1577 0.1992
REMARK   3     9  2.2300 -  2.1400    0.97     7766   139  0.1593 0.1979
REMARK   3    10  2.1400 -  2.0700    0.96     7712   138  0.1616 0.1789
REMARK   3    11  2.0700 -  2.0100    0.95     7602   139  0.1655 0.1863
REMARK   3    12  2.0100 -  1.9500    0.92     7305   131  0.1836 0.1891
REMARK   3    13  1.9500 -  1.9000    0.87     6974   119  0.1948 0.2021
REMARK   3    14  1.9000 -  1.8500    0.79     6236   118  0.2086 0.2720
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.133
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 17.047
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 19.83
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 23.05
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.010           7979
REMARK   3   ANGLE     :  1.149          10832
REMARK   3   CHIRALITY :  0.179           1219
REMARK   3   PLANARITY :  0.008           1400
REMARK   3   DIHEDRAL  : 14.042           1087
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : 1
REMARK   3   NCS GROUP : ens_1
REMARK   3    NCS OPERATOR : 1
REMARK   3     REFERENCE SELECTION: NULL
REMARK   3     SELECTION          : (chain "A" and (resid 1 through 3 or
REMARK   3                          resid 5 through 69 or resid 71 through 86
REMARK   3                          or resid 88 through 113 or resid 115
REMARK   3                          through 219 or resid 221 through 242 or
REMARK   3                          resid 244 through 251 or resid 301))
REMARK   3     ATOM PAIRS NUMBER  : NULL
REMARK   3     RMSD               : NULL
REMARK   3    NCS OPERATOR : 2
REMARK   3     REFERENCE SELECTION: NULL
REMARK   3     SELECTION          : (chain "B" and (resid 1 through 3 or
REMARK   3                          resid 5 through 69 or resid 71 through 86
REMARK   3                          or resid 88 through 113 or resid 115
REMARK   3                          through 219 or resid 221 through 242 or
REMARK   3                          resid 244 through 301))
REMARK   3     ATOM PAIRS NUMBER  : NULL
REMARK   3     RMSD               : NULL
REMARK   3    NCS OPERATOR : 3
REMARK   3     REFERENCE SELECTION: NULL
REMARK   3     SELECTION          : (chain "C" and (resid 1 through 3 or
REMARK   3                          resid 5 through 69 or resid 71 through 86
REMARK   3                          or resid 88 through 113 or resid 115
REMARK   3                          through 219 or resid 221 through 242 or
REMARK   3                          resid 244 through 251 or resid 301))
REMARK   3     ATOM PAIRS NUMBER  : NULL
REMARK   3     RMSD               : NULL
REMARK   3    NCS OPERATOR : 4
REMARK   3     REFERENCE SELECTION: NULL
REMARK   3     SELECTION          : (chain "D" and (resid 1 through 3 or
REMARK   3                          resid 5 through 69 or resid 71 through 86
REMARK   3                          or resid 88 through 113 or resid 115
REMARK   3                          through 219 or resid 221 through 242 or
REMARK   3                          resid 244 through 251 or resid 301))
REMARK   3     ATOM PAIRS NUMBER  : NULL
REMARK   3     RMSD               : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 7E0N COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 29-JAN-21.
REMARK 100 THE DEPOSITION ID IS D_1300020544.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 17-NOV-20
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRF
REMARK 200  BEAMLINE                       : BL17U1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9791
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS3 S 6M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XDS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 115284
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.850
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0
REMARK 200  DATA REDUNDANCY                : 11.90
REMARK 200  R MERGE                    (I) : 0.12400
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 19.5000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.85
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.88
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0
REMARK 200  DATA REDUNDANCY IN SHELL       : 11.90
REMARK 200  R MERGE FOR SHELL          (I) : 0.80200
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 4.000
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: 5XKS
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 57.38
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.89
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: POTASSIUM PHOSPHATE DIBASIC, SODIUM
REMARK 280  PHOSPHATE MONOBASIC, IMIDAZOLE/HYDROCHLORIC ACID, VAPOR
REMARK 280  DIFFUSION, HANGING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z
REMARK 290       3555   -X+1/2,Y+1/2,-Z
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       32.39900
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       49.92700
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       32.39900
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       49.92700
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH A 661  LIES ON A SPECIAL POSITION.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     SER A   252
REMARK 465     SER A   253
REMARK 465     LEU A   254
REMARK 465     GLN A   255
REMARK 465     GLY A   256
REMARK 465     LEU A   257
REMARK 465     GLU A   258
REMARK 465     HIS A   259
REMARK 465     HIS A   260
REMARK 465     HIS A   261
REMARK 465     HIS A   262
REMARK 465     HIS A   263
REMARK 465     HIS A   264
REMARK 465     SER B   252
REMARK 465     SER B   253
REMARK 465     LEU B   254
REMARK 465     GLN B   255
REMARK 465     GLY B   256
REMARK 465     LEU B   257
REMARK 465     GLU B   258
REMARK 465     HIS B   259
REMARK 465     HIS B   260
REMARK 465     HIS B   261
REMARK 465     HIS B   262
REMARK 465     HIS B   263
REMARK 465     HIS B   264
REMARK 465     SER C   252
REMARK 465     SER C   253
REMARK 465     LEU C   254
REMARK 465     GLN C   255
REMARK 465     GLY C   256
REMARK 465     LEU C   257
REMARK 465     GLU C   258
REMARK 465     HIS C   259
REMARK 465     HIS C   260
REMARK 465     HIS C   261
REMARK 465     HIS C   262
REMARK 465     HIS C   263
REMARK 465     HIS C   264
REMARK 465     SER D   252
REMARK 465     SER D   253
REMARK 465     LEU D   254
REMARK 465     GLN D   255
REMARK 465     GLY D   256
REMARK 465     LEU D   257
REMARK 465     GLU D   258
REMARK 465     HIS D   259
REMARK 465     HIS D   260
REMARK 465     HIS D   261
REMARK 465     HIS D   262
REMARK 465     HIS D   263
REMARK 465     HIS D   264
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    HOH A   632     O    HOH A   636              1.81
REMARK 500   O    HOH D   626     O    HOH D   746              1.84
REMARK 500   O    HOH D   597     O    HOH D   721              1.85
REMARK 500   O    HOH B   692     O    HOH B   721              1.86
REMARK 500   OE1  GLU C   195     O    HOH C   401              1.86
REMARK 500   O    HOH C   548     O    HOH C   681              1.88
REMARK 500   O    HOH D   407     O    HOH D   695              1.89
REMARK 500   O    HOH D   558     O    HOH D   623              1.90
REMARK 500   O    HOH C   655     O    HOH C   718              1.91
REMARK 500   O    HOH B   634     O    HOH B   653              1.91
REMARK 500   O    HOH C   665     O    HOH C   692              1.94
REMARK 500   O    HOH C   424     O    HOH C   677              1.96
REMARK 500   OD1  ASN C   185     O    HOH C   402              1.97
REMARK 500   O    HOH D   406     O    HOH D   644              1.98
REMARK 500   NZ   LYS A   155     O    HOH A   401              1.98
REMARK 500   O    HOH A   586     O    HOH A   652              1.98
REMARK 500   O    HOH A   434     O    HOH A   624              2.00
REMARK 500   O    HOH B   455     O    HOH B   699              2.01
REMARK 500   O    HOH D   680     O    HOH D   751              2.02
REMARK 500   O    HOH B   455     O    HOH B   705              2.02
REMARK 500   OE1  GLU B   156     O3   GOL B   301              2.03
REMARK 500   O    HOH B   587     O    HOH B   649              2.03
REMARK 500   O    HOH A   428     O    HOH A   436              2.03
REMARK 500   O    HOH B   560     O    HOH B   714              2.04
REMARK 500   O    HOH C   485     O    HOH C   674              2.06
REMARK 500   O    THR D   251     O    HOH D   401              2.07
REMARK 500   O    THR C   251     O    HOH C   403              2.08
REMARK 500   O    HOH D   486     O    HOH D   725              2.08
REMARK 500   O    HOH C   409     O    HOH C   633              2.11
REMARK 500   O    HOH C   618     O    HOH C   648              2.11
REMARK 500   O    HOH B   470     O    HOH B   682              2.14
REMARK 500   OD1  ASN A   185     O    HOH A   402              2.14
REMARK 500   O    HOH D   520     O    HOH D   755              2.14
REMARK 500   O    HOH B   662     O    HOH B   746              2.15
REMARK 500   OE2  GLU B    45     O    HOH B   401              2.16
REMARK 500   O    HOH D   730     O    HOH D   757              2.17
REMARK 500   O    HOH B   621     O    HOH B   705              2.17
REMARK 500   O    HOH D   485     O    HOH D   683              2.18
REMARK 500   O    HOH A   568     O    HOH A   623              2.18
REMARK 500   O    HOH A   523     O    HOH A   553              2.18
REMARK 500   O    HOH B   410     O    HOH B   682              2.18
REMARK 500   O    HOH A   636     O    HOH A   682              2.19
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   O    HOH C   641     O    HOH C   665     2555     1.93
REMARK 500   O    HOH B   568     O    HOH B   568     2665     1.97
REMARK 500   O    HOH D   440     O    HOH D   440     2665     1.97
REMARK 500   O    HOH C   641     O    HOH C   651     2555     2.09
REMARK 500   O    HOH B   540     O    HOH B   540     2765     2.14
REMARK 500   O    HOH D   632     O    HOH D   675     2665     2.14
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    VAL C 135   CB    VAL C 135   CG2    -0.174
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ASN A 185   CB  -  CA  -  C   ANGL. DEV. = -16.5 DEGREES
REMARK 500    ASN A 185   CB  -  CG  -  ND2 ANGL. DEV. = -16.5 DEGREES
REMARK 500    THR A 251   OG1 -  CB  -  CG2 ANGL. DEV. =  14.4 DEGREES
REMARK 500    VAL C 135   CG1 -  CB  -  CG2 ANGL. DEV. =  10.1 DEGREES
REMARK 500    ASN C 185   CB  -  CA  -  C   ANGL. DEV. = -21.8 DEGREES
REMARK 500    ARG D 247   CD  -  NE  -  CZ  ANGL. DEV. =  15.0 DEGREES
REMARK 500    ARG D 247   NE  -  CZ  -  NH1 ANGL. DEV. =   6.7 DEGREES
REMARK 500    ARG D 247   NE  -  CZ  -  NH2 ANGL. DEV. =  -7.6 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    THR A  30       -3.79     70.89
REMARK 500    THR A  60      -77.49   -124.08
REMARK 500    SER A  97     -117.29     66.60
REMARK 500    LEU A 128      -64.46    -91.56
REMARK 500    ASP A 137      126.07    -37.37
REMARK 500    TYR A 225     -147.40    -97.90
REMARK 500    THR B  30       -3.69     69.01
REMARK 500    THR B  60      -74.70   -120.56
REMARK 500    SER B  97     -116.67     63.62
REMARK 500    TYR B 225     -148.45    -98.42
REMARK 500    THR C  30       -3.98     71.75
REMARK 500    THR C  60      -76.14   -122.67
REMARK 500    SER C  97     -117.26     66.40
REMARK 500    VAL C 198      -51.16   -121.60
REMARK 500    TYR C 225     -149.59    -98.20
REMARK 500    THR D  30       -5.02     72.12
REMARK 500    THR D  60      -75.27   -123.89
REMARK 500    SER D  97     -117.01     66.32
REMARK 500    TYR D 225     -147.89    -99.45
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS
REMARK 500
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.
REMARK 500                                 MODEL     OMEGA
REMARK 500 GLU C  250     THR C  251                  137.69
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: MAIN CHAIN PLANARITY
REMARK 500
REMARK 500 THE FOLLOWING RESIDUES HAVE A PSEUDO PLANARITY
REMARK 500 TORSION ANGLE, C(I) - CA(I) - N(I+1) - O(I), GREATER
REMARK 500 10.0 DEGREES. (M=MODEL NUMBER; RES=RESIDUE NAME;
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 500 I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        ANGLE
REMARK 500    ASN A 185         11.15
REMARK 500    ASN C 185         17.96
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A 726        DISTANCE =  5.89 ANGSTROMS
REMARK 525    HOH D 794        DISTANCE =  6.39 ANGSTROMS
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL B 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL C 301
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL D 301
REMARK 999
REMARK 999 SEQUENCE
REMARK 999 THE SEQUENCE OF RESIDUE 1-160 IS GENBANK ENTRY AKM18206.1.
DBREF  7E0N A    1   160  PDB    7E0N     7E0N             1    160
DBREF1 7E0N A  161   256  UNP                  A0A160MIM0_9BACI
DBREF2 7E0N A     A0A160MIM0                        159         254
DBREF  7E0N B    1   160  PDB    7E0N     7E0N             1    160
DBREF1 7E0N B  161   256  UNP                  A0A160MIM0_9BACI
DBREF2 7E0N B     A0A160MIM0                        159         254
DBREF  7E0N C    1   160  PDB    7E0N     7E0N             1    160
DBREF1 7E0N C  161   256  UNP                  A0A160MIM0_9BACI
DBREF2 7E0N C     A0A160MIM0                        159         254
DBREF  7E0N D    1   160  PDB    7E0N     7E0N             1    160
DBREF1 7E0N D  161   256  UNP                  A0A160MIM0_9BACI
DBREF2 7E0N D     A0A160MIM0                        159         254
SEQADV 7E0N LEU A  257  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N GLU A  258  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS A  259  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS A  260  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS A  261  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS A  262  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS A  263  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS A  264  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N LEU B  257  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N GLU B  258  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS B  259  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS B  260  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS B  261  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS B  262  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS B  263  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS B  264  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N LEU C  257  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N GLU C  258  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS C  259  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS C  260  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS C  261  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS C  262  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS C  263  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS C  264  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N LEU D  257  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N GLU D  258  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS D  259  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS D  260  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS D  261  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS D  262  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS D  263  UNP  A0A160MIM           EXPRESSION TAG
SEQADV 7E0N HIS D  264  UNP  A0A160MIM           EXPRESSION TAG
SEQRES   1 A  264  MET THR GLU THR TYR PRO VAL VAL LYS GLY ALA GLU PRO
SEQRES   2 A  264  PHE PHE PHE GLU GLY ASN ASP ILE GLY ILE LEU VAL LEU
SEQRES   3 A  264  HIS GLY PHE THR GLY SER PRO GLN SER MET ARG PRO LEU
SEQRES   4 A  264  GLY GLU ALA TYR HIS GLU ALA GLY TYR THR VAL CYS GLY
SEQRES   5 A  264  PRO ARG LEU LYS GLY HIS GLY THR HIS TYR GLU ASP MET
SEQRES   6 A  264  GLU LYS THR THR CYS GLN ASP TRP ILE ASP SER VAL GLU
SEQRES   7 A  264  ALA GLY TYR GLU TRP LEU LYS ASN ARG CYS GLY THR ILE
SEQRES   8 A  264  PHE VAL THR GLY LEU SER MET GLY GLY THR LEU THR LEU
SEQRES   9 A  264  TYR MET ALA GLU HIS HIS PRO GLU ILE CYS GLY ILE ALA
SEQRES  10 A  264  PRO ILE ASN ALA ALA ILE ASN MET PRO ALA LEU ALA GLY
SEQRES  11 A  264  ALA LEU ALA GLY VAL GLY ASP LEU PRO ARG PHE LEU ASP
SEQRES  12 A  264  ALA ILE GLY SER ASP ILE LYS LYS PRO GLY VAL LYS GLU
SEQRES  13 A  264  LEU ALA TYR GLU LYS THR PRO VAL LYS SER ILE GLY GLU
SEQRES  14 A  264  ILE THR GLU LEU MET LYS LYS VAL LYS GLY ASP LEU GLU
SEQRES  15 A  264  LYS VAL ASN CYS PRO ALA LEU ILE PHE VAL SER LYS GLU
SEQRES  16 A  264  ASP HIS VAL VAL PRO PRO SER ASN SER GLN GLU ILE TYR
SEQRES  17 A  264  SER SER ILE LYS SER ALA ALA LYS GLU LEU VAL THR LEU
SEQRES  18 A  264  ASP ASN SER TYR HIS VAL ALA THR LEU ASP ASN ASP GLN
SEQRES  19 A  264  ASP ILE ILE ILE GLU ARG THR LEU HIS PHE LEU GLN ARG
SEQRES  20 A  264  VAL LEU GLU THR SER SER LEU GLN GLY LEU GLU HIS HIS
SEQRES  21 A  264  HIS HIS HIS HIS
SEQRES   1 B  264  MET THR GLU THR TYR PRO VAL VAL LYS GLY ALA GLU PRO
SEQRES   2 B  264  PHE PHE PHE GLU GLY ASN ASP ILE GLY ILE LEU VAL LEU
SEQRES   3 B  264  HIS GLY PHE THR GLY SER PRO GLN SER MET ARG PRO LEU
SEQRES   4 B  264  GLY GLU ALA TYR HIS GLU ALA GLY TYR THR VAL CYS GLY
SEQRES   5 B  264  PRO ARG LEU LYS GLY HIS GLY THR HIS TYR GLU ASP MET
SEQRES   6 B  264  GLU LYS THR THR CYS GLN ASP TRP ILE ASP SER VAL GLU
SEQRES   7 B  264  ALA GLY TYR GLU TRP LEU LYS ASN ARG CYS GLY THR ILE
SEQRES   8 B  264  PHE VAL THR GLY LEU SER MET GLY GLY THR LEU THR LEU
SEQRES   9 B  264  TYR MET ALA GLU HIS HIS PRO GLU ILE CYS GLY ILE ALA
SEQRES  10 B  264  PRO ILE ASN ALA ALA ILE ASN MET PRO ALA LEU ALA GLY
SEQRES  11 B  264  ALA LEU ALA GLY VAL GLY ASP LEU PRO ARG PHE LEU ASP
SEQRES  12 B  264  ALA ILE GLY SER ASP ILE LYS LYS PRO GLY VAL LYS GLU
SEQRES  13 B  264  LEU ALA TYR GLU LYS THR PRO VAL LYS SER ILE GLY GLU
SEQRES  14 B  264  ILE THR GLU LEU MET LYS LYS VAL LYS GLY ASP LEU GLU
SEQRES  15 B  264  LYS VAL ASN CYS PRO ALA LEU ILE PHE VAL SER LYS GLU
SEQRES  16 B  264  ASP HIS VAL VAL PRO PRO SER ASN SER GLN GLU ILE TYR
SEQRES  17 B  264  SER SER ILE LYS SER ALA ALA LYS GLU LEU VAL THR LEU
SEQRES  18 B  264  ASP ASN SER TYR HIS VAL ALA THR LEU ASP ASN ASP GLN
SEQRES  19 B  264  ASP ILE ILE ILE GLU ARG THR LEU HIS PHE LEU GLN ARG
SEQRES  20 B  264  VAL LEU GLU THR SER SER LEU GLN GLY LEU GLU HIS HIS
SEQRES  21 B  264  HIS HIS HIS HIS
SEQRES   1 C  264  MET THR GLU THR TYR PRO VAL VAL LYS GLY ALA GLU PRO
SEQRES   2 C  264  PHE PHE PHE GLU GLY ASN ASP ILE GLY ILE LEU VAL LEU
SEQRES   3 C  264  HIS GLY PHE THR GLY SER PRO GLN SER MET ARG PRO LEU
SEQRES   4 C  264  GLY GLU ALA TYR HIS GLU ALA GLY TYR THR VAL CYS GLY
SEQRES   5 C  264  PRO ARG LEU LYS GLY HIS GLY THR HIS TYR GLU ASP MET
SEQRES   6 C  264  GLU LYS THR THR CYS GLN ASP TRP ILE ASP SER VAL GLU
SEQRES   7 C  264  ALA GLY TYR GLU TRP LEU LYS ASN ARG CYS GLY THR ILE
SEQRES   8 C  264  PHE VAL THR GLY LEU SER MET GLY GLY THR LEU THR LEU
SEQRES   9 C  264  TYR MET ALA GLU HIS HIS PRO GLU ILE CYS GLY ILE ALA
SEQRES  10 C  264  PRO ILE ASN ALA ALA ILE ASN MET PRO ALA LEU ALA GLY
SEQRES  11 C  264  ALA LEU ALA GLY VAL GLY ASP LEU PRO ARG PHE LEU ASP
SEQRES  12 C  264  ALA ILE GLY SER ASP ILE LYS LYS PRO GLY VAL LYS GLU
SEQRES  13 C  264  LEU ALA TYR GLU LYS THR PRO VAL LYS SER ILE GLY GLU
SEQRES  14 C  264  ILE THR GLU LEU MET LYS LYS VAL LYS GLY ASP LEU GLU
SEQRES  15 C  264  LYS VAL ASN CYS PRO ALA LEU ILE PHE VAL SER LYS GLU
SEQRES  16 C  264  ASP HIS VAL VAL PRO PRO SER ASN SER GLN GLU ILE TYR
SEQRES  17 C  264  SER SER ILE LYS SER ALA ALA LYS GLU LEU VAL THR LEU
SEQRES  18 C  264  ASP ASN SER TYR HIS VAL ALA THR LEU ASP ASN ASP GLN
SEQRES  19 C  264  ASP ILE ILE ILE GLU ARG THR LEU HIS PHE LEU GLN ARG
SEQRES  20 C  264  VAL LEU GLU THR SER SER LEU GLN GLY LEU GLU HIS HIS
SEQRES  21 C  264  HIS HIS HIS HIS
SEQRES   1 D  264  MET THR GLU THR TYR PRO VAL VAL LYS GLY ALA GLU PRO
SEQRES   2 D  264  PHE PHE PHE GLU GLY ASN ASP ILE GLY ILE LEU VAL LEU
SEQRES   3 D  264  HIS GLY PHE THR GLY SER PRO GLN SER MET ARG PRO LEU
SEQRES   4 D  264  GLY GLU ALA TYR HIS GLU ALA GLY TYR THR VAL CYS GLY
SEQRES   5 D  264  PRO ARG LEU LYS GLY HIS GLY THR HIS TYR GLU ASP MET
SEQRES   6 D  264  GLU LYS THR THR CYS GLN ASP TRP ILE ASP SER VAL GLU
SEQRES   7 D  264  ALA GLY TYR GLU TRP LEU LYS ASN ARG CYS GLY THR ILE
SEQRES   8 D  264  PHE VAL THR GLY LEU SER MET GLY GLY THR LEU THR LEU
SEQRES   9 D  264  TYR MET ALA GLU HIS HIS PRO GLU ILE CYS GLY ILE ALA
SEQRES  10 D  264  PRO ILE ASN ALA ALA ILE ASN MET PRO ALA LEU ALA GLY
SEQRES  11 D  264  ALA LEU ALA GLY VAL GLY ASP LEU PRO ARG PHE LEU ASP
SEQRES  12 D  264  ALA ILE GLY SER ASP ILE LYS LYS PRO GLY VAL LYS GLU
SEQRES  13 D  264  LEU ALA TYR GLU LYS THR PRO VAL LYS SER ILE GLY GLU
SEQRES  14 D  264  ILE THR GLU LEU MET LYS LYS VAL LYS GLY ASP LEU GLU
SEQRES  15 D  264  LYS VAL ASN CYS PRO ALA LEU ILE PHE VAL SER LYS GLU
SEQRES  16 D  264  ASP HIS VAL VAL PRO PRO SER ASN SER GLN GLU ILE TYR
SEQRES  17 D  264  SER SER ILE LYS SER ALA ALA LYS GLU LEU VAL THR LEU
SEQRES  18 D  264  ASP ASN SER TYR HIS VAL ALA THR LEU ASP ASN ASP GLN
SEQRES  19 D  264  ASP ILE ILE ILE GLU ARG THR LEU HIS PHE LEU GLN ARG
SEQRES  20 D  264  VAL LEU GLU THR SER SER LEU GLN GLY LEU GLU HIS HIS
SEQRES  21 D  264  HIS HIS HIS HIS
HET    GOL  A 301       6
HET    GOL  B 301       6
HET    GOL  C 301       6
HET    GOL  D 301       6
HETNAM     GOL GLYCEROL
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL
FORMUL   5  GOL    4(C3 H8 O3)
FORMUL   9  HOH   *1409(H2 O)
HELIX    1 AA1 SER A   32  SER A   35  5                                   4
HELIX    2 AA2 MET A   36  ALA A   46  1                                  11
HELIX    3 AA3 HIS A   61  LYS A   67  1                                   7
HELIX    4 AA4 THR A   69  CYS A   88  1                                  20
HELIX    5 AA5 SER A   97  HIS A  110  1                                  14
HELIX    6 AA6 LEU A  128  GLY A  134  5                                   7
HELIX    7 AA7 VAL A  164  ASP A  180  1                                  17
HELIX    8 AA8 LEU A  181  VAL A  184  5                                   4
HELIX    9 AA9 PRO A  201  ILE A  211  1                                  11
HELIX   10 AB1 VAL A  227  ASP A  231  5                                   5
HELIX   11 AB2 ASP A  233  THR A  251  1                                  19
HELIX   12 AB3 SER B   32  SER B   35  5                                   4
HELIX   13 AB4 MET B   36  ALA B   46  1                                  11
HELIX   14 AB5 HIS B   61  LYS B   67  1                                   7
HELIX   15 AB6 THR B   69  CYS B   88  1                                  20
HELIX   16 AB7 SER B   97  HIS B  110  1                                  14
HELIX   17 AB8 MET B  125  GLY B  130  1                                   6
HELIX   18 AB9 ALA B  131  GLY B  134  5                                   4
HELIX   19 AC1 VAL B  164  ASP B  180  1                                  17
HELIX   20 AC2 LEU B  181  VAL B  184  5                                   4
HELIX   21 AC3 PRO B  201  ILE B  211  1                                  11
HELIX   22 AC4 VAL B  227  ASP B  231  5                                   5
HELIX   23 AC5 ASP B  233  THR B  251  1                                  19
HELIX   24 AC6 SER C   32  SER C   35  5                                   4
HELIX   25 AC7 MET C   36  ALA C   46  1                                  11
HELIX   26 AC8 HIS C   61  LYS C   67  1                                   7
HELIX   27 AC9 THR C   69  CYS C   88  1                                  20
HELIX   28 AD1 SER C   97  HIS C  110  1                                  14
HELIX   29 AD2 MET C  125  GLY C  130  1                                   6
HELIX   30 AD3 ALA C  131  GLY C  134  5                                   4
HELIX   31 AD4 VAL C  164  ASP C  180  1                                  17
HELIX   32 AD5 LEU C  181  VAL C  184  5                                   4
HELIX   33 AD6 PRO C  201  ILE C  211  1                                  11
HELIX   34 AD7 VAL C  227  ASP C  231  5                                   5
HELIX   35 AD8 ASP C  233  THR C  251  1                                  19
HELIX   36 AD9 SER D   32  SER D   35  5                                   4
HELIX   37 AE1 MET D   36  ALA D   46  1                                  11
HELIX   38 AE2 HIS D   61  LYS D   67  1                                   7
HELIX   39 AE3 THR D   69  CYS D   88  1                                  20
HELIX   40 AE4 SER D   97  HIS D  110  1                                  14
HELIX   41 AE5 MET D  125  GLY D  130  1                                   6
HELIX   42 AE6 ALA D  131  GLY D  134  5                                   4
HELIX   43 AE7 VAL D  164  ASP D  180  1                                  17
HELIX   44 AE8 LEU D  181  VAL D  184  5                                   4
HELIX   45 AE9 PRO D  201  ILE D  211  1                                  11
HELIX   46 AF1 VAL D  227  ASP D  231  5                                   5
HELIX   47 AF2 ASP D  233  THR D  251  1                                  19
SHEET    1 AA1 5 PHE A  14  PHE A  16  0
SHEET    2 AA1 5 THR A  49  GLY A  52 -1  O  GLY A  52   N  PHE A  14
SHEET    3 AA1 5 ILE A  21  LEU A  26  1  N  VAL A  25   O  CYS A  51
SHEET    4 AA1 5 THR A  90  LEU A  96  1  O  THR A  90   N  GLY A  22
SHEET    5 AA1 5 GLY A 115  ILE A 119  1  O  ILE A 119   N  GLY A  95
SHEET    1 AA2 2 PHE A 141  ASP A 143  0
SHEET    2 AA2 2 LYS A 161  PRO A 163 -1  O  THR A 162   N  LEU A 142
SHEET    1 AA3 2 ALA A 188  SER A 193  0
SHEET    2 AA3 2 LYS A 216  LEU A 221  1  O  GLU A 217   N  ILE A 190
SHEET    1 AA4 5 PHE B  14  PHE B  16  0
SHEET    2 AA4 5 THR B  49  GLY B  52 -1  O  GLY B  52   N  PHE B  14
SHEET    3 AA4 5 ILE B  21  LEU B  26  1  N  VAL B  25   O  CYS B  51
SHEET    4 AA4 5 THR B  90  LEU B  96  1  O  THR B  90   N  GLY B  22
SHEET    5 AA4 5 GLY B 115  ILE B 119  1  O  ILE B 119   N  GLY B  95
SHEET    1 AA5 2 PHE B 141  ASP B 143  0
SHEET    2 AA5 2 LYS B 161  PRO B 163 -1  O  THR B 162   N  LEU B 142
SHEET    1 AA6 2 ALA B 188  SER B 193  0
SHEET    2 AA6 2 LYS B 216  LEU B 221  1  O  GLU B 217   N  ILE B 190
SHEET    1 AA7 5 PHE C  14  PHE C  16  0
SHEET    2 AA7 5 THR C  49  GLY C  52 -1  O  GLY C  52   N  PHE C  14
SHEET    3 AA7 5 ILE C  21  LEU C  26  1  N  VAL C  25   O  CYS C  51
SHEET    4 AA7 5 THR C  90  LEU C  96  1  O  THR C  90   N  GLY C  22
SHEET    5 AA7 5 GLY C 115  ILE C 119  1  O  ILE C 119   N  GLY C  95
SHEET    1 AA8 2 PHE C 141  ASP C 143  0
SHEET    2 AA8 2 LYS C 161  PRO C 163 -1  O  THR C 162   N  LEU C 142
SHEET    1 AA9 2 ALA C 188  SER C 193  0
SHEET    2 AA9 2 LYS C 216  LEU C 221  1  O  GLU C 217   N  ILE C 190
SHEET    1 AB1 5 PHE D  14  PHE D  16  0
SHEET    2 AB1 5 THR D  49  GLY D  52 -1  O  GLY D  52   N  PHE D  14
SHEET    3 AB1 5 ILE D  21  LEU D  26  1  N  VAL D  25   O  CYS D  51
SHEET    4 AB1 5 THR D  90  LEU D  96  1  O  THR D  90   N  GLY D  22
SHEET    5 AB1 5 GLY D 115  ILE D 119  1  O  ILE D 119   N  GLY D  95
SHEET    1 AB2 2 PHE D 141  ASP D 143  0
SHEET    2 AB2 2 LYS D 161  PRO D 163 -1  O  THR D 162   N  LEU D 142
SHEET    1 AB3 2 ALA D 188  SER D 193  0
SHEET    2 AB3 2 LYS D 216  LEU D 221  1  O  VAL D 219   N  ILE D 190
SITE     1 AC1 10 PHE A  29  GLY A  31  SER A  97  ILE A 145
SITE     2 AC1 10 GLY A 146  GLU A 156  HIS A 226  HOH A 406
SITE     3 AC1 10 HOH A 442  HOH A 492
SITE     1 AC2  8 PHE B  29  GLY B  31  SER B  97  ILE B 145
SITE     2 AC2  8 GLY B 146  GLU B 156  HIS B 226  HOH B 416
SITE     1 AC3  9 PHE C  29  GLY C  31  SER C  97  GLY C 146
SITE     2 AC3  9 GLU C 156  HIS C 226  HOH C 408  HOH C 496
SITE     3 AC3  9 HOH C 527
SITE     1 AC4 10 PHE D  29  GLY D  31  SER D  97  GLY D 146
SITE     2 AC4 10 GLU D 156  HIS D 226  VAL D 227  HOH D 441
SITE     3 AC4 10 HOH D 506  HOH D 516
CRYST1   64.798   99.854  207.236  90.00  90.00  90.00 P 21 21 2    16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.015433  0.000000  0.000000        0.00000
SCALE2      0.000000  0.010015  0.000000        0.00000
SCALE3      0.000000  0.000000  0.004825        0.00000
MTRIX1   1  0.999301  0.034734 -0.013802       31.84990    1
MTRIX2   1  0.035334 -0.998321  0.045894       50.08763    1
MTRIX3   1 -0.012185 -0.046349 -0.998851      104.86780    1
MTRIX1   2 -0.999693 -0.018196 -0.016815       24.42176    1
MTRIX2   2 -0.017453  0.998907 -0.043350        1.23178    1
MTRIX3   2  0.017585 -0.043043 -0.998918       52.55174    1
MTRIX1   3 -0.999980 -0.004835 -0.004080       56.20501    1
MTRIX2   3  0.004860 -0.999970 -0.005969       51.43757    1
MTRIX3   3 -0.004051 -0.005989  0.999974       52.03285    1
TER    1935      THR A 251
TER    3877      THR B 251
TER    5835      THR C 251
TER    7781      THR D 251
MASTER      543    0    4   47   36    0   11   15 9145    4   24   84
END