longtext: 7l50-pdb

content
HEADER    HYDROLASE/INHIBITOR                     21-DEC-20   7L50
TITLE     CRYSTAL STRUCTURE OF HUMAN MONOACYLGLYCEROL LIPASE IN COMPLEX WITH
TITLE    2 COMPOUND 1
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: MONOGLYCERIDE LIPASE;
COMPND   3 CHAIN: A, B, C, D;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE   3 ORGANISM_COMMON: HUMAN;
SOURCE   4 ORGANISM_TAXID: 9606;
SOURCE   5 GENE: MGLL;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    INHIBITOR, SERINE HYDROLASE, HYDROLASE, HYDROLASE-INHIBITOR COMPLEX
EXPDTA    X-RAY DIFFRACTION
AUTHOR    L.QIN,W.LANE,R.J.SKENE
REVDAT   1   11-AUG-21 7L50    0
JRNL        AUTH   S.IKEDA,H.SUGIYAMA,H.TOKUHARA,M.MURAKAMI,M.NAKAMURA,Y.OGURO,
JRNL        AUTH 2 J.AIDA,N.MORISHITA,S.SOGABE,D.R.DOUGAN,S.C.GAY,L.QIN,
JRNL        AUTH 3 N.ARIMURA,Y.TAKAHASHI,M.SASAKI,Y.KAMADA,K.AOYAMA,K.KIMOTO,
JRNL        AUTH 4 M.KAMATA
JRNL        TITL   DESIGN AND SYNTHESIS OF NOVEL SPIRO DERIVATIVES AS POTENT
JRNL        TITL 2 AND REVERSIBLE MONOACYLGLYCEROL LIPASE (MAGL) INHIBITORS:
JRNL        TITL 3 BIOISOSTERIC TRANSFORMATION FROM 3-OXO-3,4-DIHYDRO-2 H
JRNL        TITL 4 -BENZO[ B ][1,4]OXAZIN-6-YL MOIETY.
JRNL        REF    J.MED.CHEM.                                2021
JRNL        REFN                   ISSN 0022-2623
JRNL        PMID   34328319
JRNL        DOI    10.1021/ACS.JMEDCHEM.1C00432
REMARK   2
REMARK   2 RESOLUTION.    2.30 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.8.0257
REMARK   3   AUTHORS     : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK   3               : NICHOLLS,WINN,LONG,VAGIN
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.30
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 48.19
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.7
REMARK   3   NUMBER OF REFLECTIONS             : 78322
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : FREE R-VALUE
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL
REMARK   3   R VALUE     (WORKING + TEST SET) : NULL
REMARK   3   R VALUE            (WORKING SET) : 0.239
REMARK   3   FREE R VALUE                     : 0.269
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.895
REMARK   3   FREE R VALUE TEST SET COUNT      : 3834
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.30
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.36
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 5379
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 95.86
REMARK   3   BIN R VALUE           (WORKING SET) : 0.4420
REMARK   3   BIN FREE R VALUE SET COUNT          : 291
REMARK   3   BIN FREE R VALUE                    : 0.4060
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 8864
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 132
REMARK   3   SOLVENT ATOMS            : 157
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 82.05
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : -2.25700
REMARK   3    B22 (A**2) : -8.10900
REMARK   3    B33 (A**2) : 7.50100
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : -2.69400
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.277
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.222
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.304
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 31.945
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.957
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.941
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  9209 ; 0.004 ; 0.012
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 12504 ; 1.127 ; 1.645
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  1138 ; 5.960 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   436 ;30.528 ;21.743
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1506 ;15.327 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    55 ;15.903 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1150 ; 0.088 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  7046 ; 0.004 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  4378 ; 0.214 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  6274 ; 0.303 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   307 ; 0.128 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  4584 ; 2.564 ; 4.322
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  5706 ; 4.275 ; 6.456
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  4625 ; 2.918 ; 4.346
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  6782 ; 4.708 ; 6.463
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 4
REMARK   3
REMARK   3   TLS GROUP : 1
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A     1        A   701
REMARK   3    ORIGIN FOR THE GROUP (A):  97.6509 -33.2935 167.5382
REMARK   3    T TENSOR
REMARK   3      T11:   0.1933 T22:   0.4465
REMARK   3      T33:   0.6496 T12:   0.0067
REMARK   3      T13:   0.0649 T23:   0.0169
REMARK   3    L TENSOR
REMARK   3      L11:   0.5447 L22:   2.3501
REMARK   3      L33:   0.1320 L12:   0.0606
REMARK   3      L13:   0.1750 L23:   0.3806
REMARK   3    S TENSOR
REMARK   3      S11:   0.0227 S12:  -0.0338 S13:   0.2427
REMARK   3      S21:  -0.1200 S22:  -0.0688 S23:   0.0303
REMARK   3      S31:   0.0438 S32:  -0.0031 S33:   0.0461
REMARK   3
REMARK   3   TLS GROUP : 2
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   B     1        B   701
REMARK   3    ORIGIN FOR THE GROUP (A): 129.0034 -11.2513 177.2518
REMARK   3    T TENSOR
REMARK   3      T11:   0.1814 T22:   0.4245
REMARK   3      T33:   0.6677 T12:  -0.0032
REMARK   3      T13:   0.0622 T23:   0.0034
REMARK   3    L TENSOR
REMARK   3      L11:   0.5693 L22:   2.7596
REMARK   3      L33:   0.0604 L12:   0.1347
REMARK   3      L13:  -0.0861 L23:   0.3114
REMARK   3    S TENSOR
REMARK   3      S11:   0.0897 S12:   0.0574 S13:  -0.1842
REMARK   3      S21:   0.1766 S22:  -0.0694 S23:  -0.1183
REMARK   3      S31:  -0.0268 S32:  -0.0127 S33:  -0.0204
REMARK   3
REMARK   3   TLS GROUP : 3
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   C     1        C   701
REMARK   3    ORIGIN FOR THE GROUP (A): 112.2235  27.7195 119.7576
REMARK   3    T TENSOR
REMARK   3      T11:   0.7744 T22:   0.0385
REMARK   3      T33:   0.4616 T12:  -0.0192
REMARK   3      T13:   0.4844 T23:  -0.0313
REMARK   3    L TENSOR
REMARK   3      L11:   0.7863 L22:   2.1775
REMARK   3      L33:   0.0994 L12:  -0.2595
REMARK   3      L13:   0.2212 L23:  -0.2797
REMARK   3    S TENSOR
REMARK   3      S11:   0.1012 S12:  -0.0202 S13:   0.2342
REMARK   3      S21:   0.0490 S22:  -0.1207 S23:   0.0063
REMARK   3      S31:   0.0478 S32:   0.0115 S33:   0.0195
REMARK   3
REMARK   3   TLS GROUP : 4
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   D     1        D   701
REMARK   3    ORIGIN FOR THE GROUP (A): 112.3432 -13.7347 119.5722
REMARK   3    T TENSOR
REMARK   3      T11:   0.8088 T22:   0.0175
REMARK   3      T33:   0.4391 T12:  -0.0079
REMARK   3      T13:   0.5147 T23:   0.0067
REMARK   3    L TENSOR
REMARK   3      L11:   0.8644 L22:   2.7054
REMARK   3      L33:   0.0120 L12:   0.1649
REMARK   3      L13:  -0.0753 L23:   0.0611
REMARK   3    S TENSOR
REMARK   3      S11:   0.0870 S12:  -0.0265 S13:  -0.1880
REMARK   3      S21:   0.0934 S22:  -0.0984 S23:  -0.0162
REMARK   3      S31:  -0.0375 S32:   0.0002 S33:   0.0114
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK BULK SOLVENT
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THEIR
REMARK   3  RIDING POSITIONS
REMARK   4
REMARK   4 7L50 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 23-DEC-20.
REMARK 100 THE DEPOSITION ID IS D_1000253721.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 12-MAR-17
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : APS
REMARK 200  BEAMLINE                       : 23-ID-B
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.0
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER X 16M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 79270
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.300
REMARK 200  RESOLUTION RANGE LOW       (A) : 49.060
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.9
REMARK 200  DATA REDUNDANCY                : 3.900
REMARK 200  R MERGE                    (I) : 0.12300
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 4.1000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.30
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.35
REMARK 200  COMPLETENESS FOR SHELL     (%) : 97.9
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.70
REMARK 200  R MERGE FOR SHELL          (I) : 0.72800
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: 5ZUN
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 62.26
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.26
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M NAOAC PH 5.5, 32-38% MPD, VAPOR
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       63.58400
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A   -16
REMARK 465     HIS A   -15
REMARK 465     HIS A   -14
REMARK 465     HIS A   -13
REMARK 465     HIS A   -12
REMARK 465     HIS A   -11
REMARK 465     HIS A   -10
REMARK 465     GLY A    -9
REMARK 465     SER A    -8
REMARK 465     GLU A    -7
REMARK 465     ASN A    -6
REMARK 465     LEU A    -5
REMARK 465     TYR A    -4
REMARK 465     PHE A    -3
REMARK 465     GLN A    -2
REMARK 465     GLY A    -1
REMARK 465     SER A     0
REMARK 465     LEU A   167
REMARK 465     ASN A   168
REMARK 465     SER A   169
REMARK 465     VAL A   170
REMARK 465     LEU A   171
REMARK 465     PRO A   172
REMARK 465     ASN A   173
REMARK 465     LEU A   174
REMARK 465     SER A   175
REMARK 465     SER A   176
REMARK 465     THR A   296
REMARK 465     ALA A   297
REMARK 465     GLY A   298
REMARK 465     THR A   299
REMARK 465     ALA A   300
REMARK 465     SER A   301
REMARK 465     PRO A   302
REMARK 465     PRO A   303
REMARK 465     MET B   -16
REMARK 465     HIS B   -15
REMARK 465     HIS B   -14
REMARK 465     HIS B   -13
REMARK 465     HIS B   -12
REMARK 465     HIS B   -11
REMARK 465     HIS B   -10
REMARK 465     GLY B    -9
REMARK 465     SER B    -8
REMARK 465     GLU B    -7
REMARK 465     ASN B    -6
REMARK 465     LEU B    -5
REMARK 465     TYR B    -4
REMARK 465     PHE B    -3
REMARK 465     GLN B    -2
REMARK 465     GLY B    -1
REMARK 465     SER B     0
REMARK 465     LEU B   167
REMARK 465     ASN B   168
REMARK 465     SER B   169
REMARK 465     VAL B   170
REMARK 465     LEU B   171
REMARK 465     THR B   296
REMARK 465     ALA B   297
REMARK 465     GLY B   298
REMARK 465     THR B   299
REMARK 465     ALA B   300
REMARK 465     SER B   301
REMARK 465     PRO B   302
REMARK 465     PRO B   303
REMARK 465     MET C   -16
REMARK 465     HIS C   -15
REMARK 465     HIS C   -14
REMARK 465     HIS C   -13
REMARK 465     HIS C   -12
REMARK 465     HIS C   -11
REMARK 465     HIS C   -10
REMARK 465     GLY C    -9
REMARK 465     SER C    -8
REMARK 465     GLU C    -7
REMARK 465     ASN C    -6
REMARK 465     LEU C    -5
REMARK 465     TYR C    -4
REMARK 465     PHE C    -3
REMARK 465     GLN C    -2
REMARK 465     GLY C    -1
REMARK 465     SER C     0
REMARK 465     LEU C   167
REMARK 465     ASN C   168
REMARK 465     SER C   169
REMARK 465     VAL C   170
REMARK 465     LEU C   171
REMARK 465     PRO C   172
REMARK 465     ASN C   173
REMARK 465     LEU C   174
REMARK 465     SER C   175
REMARK 465     THR C   296
REMARK 465     ALA C   297
REMARK 465     GLY C   298
REMARK 465     THR C   299
REMARK 465     ALA C   300
REMARK 465     SER C   301
REMARK 465     PRO C   302
REMARK 465     PRO C   303
REMARK 465     MET D   -16
REMARK 465     HIS D   -15
REMARK 465     HIS D   -14
REMARK 465     HIS D   -13
REMARK 465     HIS D   -12
REMARK 465     HIS D   -11
REMARK 465     HIS D   -10
REMARK 465     GLY D    -9
REMARK 465     SER D    -8
REMARK 465     GLU D    -7
REMARK 465     ASN D    -6
REMARK 465     LEU D    -5
REMARK 465     TYR D    -4
REMARK 465     PHE D    -3
REMARK 465     GLN D    -2
REMARK 465     GLY D    -1
REMARK 465     SER D     0
REMARK 465     LEU D   167
REMARK 465     ASN D   168
REMARK 465     SER D   169
REMARK 465     VAL D   170
REMARK 465     LEU D   171
REMARK 465     PRO D   172
REMARK 465     ASN D   173
REMARK 465     LEU D   174
REMARK 465     SER D   175
REMARK 465     ALA D   295
REMARK 465     THR D   296
REMARK 465     ALA D   297
REMARK 465     GLY D   298
REMARK 465     THR D   299
REMARK 465     ALA D   300
REMARK 465     SER D   301
REMARK 465     PRO D   302
REMARK 465     PRO D   303
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     ARG A 202    CG   CD   NE   CZ   NH1  NH2
REMARK 470     GLU B 154    CG   CD   OE1  OE2
REMARK 470     LYS B 165    CG   CD   CE   NZ
REMARK 470     GLU C 154    CG   CD   OE1  OE2
REMARK 470     GLU D 154    CG   CD   OE1  OE2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PRO A   7      150.33    -42.19
REMARK 500    GLU A  53     -152.87    -94.72
REMARK 500    ASP A  69       25.63     81.39
REMARK 500    HIS A  77      159.52    -47.12
REMARK 500    SER A 122     -129.08     54.34
REMARK 500    PRO A 225       -9.08    -55.58
REMARK 500    LEU A 241      -60.38   -108.58
REMARK 500    TYR A 268     -146.69    -86.69
REMARK 500    GLU A 274     -159.97   -109.08
REMARK 500    THR A 294      -16.56   -146.20
REMARK 500    GLU B  53     -154.35   -100.06
REMARK 500    ASP B  69       30.57     73.48
REMARK 500    ARG B  87      118.71    -39.08
REMARK 500    SER B 122     -122.53     58.66
REMARK 500    LEU B 174     -154.39   -109.06
REMARK 500    ASP B 180      105.99    -49.19
REMARK 500    TYR B 268     -140.38    -83.49
REMARK 500    GLU B 274     -159.53   -100.63
REMARK 500    THR B 294       -6.29   -149.44
REMARK 500    GLU C  53     -149.58   -105.67
REMARK 500    SER C 122     -134.44     63.27
REMARK 500    SER C 146       66.99     35.95
REMARK 500    PRO C 225       -7.19    -58.40
REMARK 500    LEU C 241      -61.54   -109.24
REMARK 500    TYR C 268     -146.17    -97.00
REMARK 500    PRO D  11       -8.56    -58.32
REMARK 500    GLU D  53     -157.61   -105.16
REMARK 500    ASP D  69       32.45     71.40
REMARK 500    SER D 122     -116.86     53.20
REMARK 500    ARG D 135       74.18   -118.64
REMARK 500    SER D 196       13.75   -140.30
REMARK 500    PRO D 225        3.11    -58.45
REMARK 500    TYR D 268     -136.66    -88.24
REMARK 500    GLU D 274     -156.53    -96.60
REMARK 500
REMARK 500 REMARK: NULL
DBREF1 7L50 A    0   303  UNP                  A0A0C4DFN3_HUMAN
DBREF2 7L50 A     A0A0C4DFN3                         10         313
DBREF1 7L50 B    0   303  UNP                  A0A0C4DFN3_HUMAN
DBREF2 7L50 B     A0A0C4DFN3                         10         313
DBREF1 7L50 C    0   303  UNP                  A0A0C4DFN3_HUMAN
DBREF2 7L50 C     A0A0C4DFN3                         10         313
DBREF1 7L50 D    0   303  UNP                  A0A0C4DFN3_HUMAN
DBREF2 7L50 D     A0A0C4DFN3                         10         313
SEQADV 7L50 MET A  -16  UNP  A0A0C4DFN           INITIATING METHIONINE
SEQADV 7L50 HIS A  -15  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS A  -14  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS A  -13  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS A  -12  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS A  -11  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS A  -10  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLY A   -9  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 SER A   -8  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLU A   -7  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 ASN A   -6  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 LEU A   -5  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 TYR A   -4  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 PHE A   -3  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLN A   -2  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLY A   -1  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 ALA A   36  UNP  A0A0C4DFN LYS    46 CONFLICT
SEQADV 7L50 SER A  169  UNP  A0A0C4DFN LEU   179 CONFLICT
SEQADV 7L50 SER A  176  UNP  A0A0C4DFN LEU   186 CONFLICT
SEQADV 7L50 MET B  -16  UNP  A0A0C4DFN           INITIATING METHIONINE
SEQADV 7L50 HIS B  -15  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS B  -14  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS B  -13  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS B  -12  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS B  -11  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS B  -10  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLY B   -9  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 SER B   -8  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLU B   -7  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 ASN B   -6  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 LEU B   -5  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 TYR B   -4  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 PHE B   -3  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLN B   -2  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLY B   -1  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 ALA B   36  UNP  A0A0C4DFN LYS    46 CONFLICT
SEQADV 7L50 SER B  169  UNP  A0A0C4DFN LEU   179 CONFLICT
SEQADV 7L50 SER B  176  UNP  A0A0C4DFN LEU   186 CONFLICT
SEQADV 7L50 MET C  -16  UNP  A0A0C4DFN           INITIATING METHIONINE
SEQADV 7L50 HIS C  -15  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS C  -14  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS C  -13  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS C  -12  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS C  -11  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS C  -10  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLY C   -9  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 SER C   -8  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLU C   -7  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 ASN C   -6  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 LEU C   -5  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 TYR C   -4  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 PHE C   -3  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLN C   -2  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLY C   -1  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 ALA C   36  UNP  A0A0C4DFN LYS    46 CONFLICT
SEQADV 7L50 SER C  169  UNP  A0A0C4DFN LEU   179 CONFLICT
SEQADV 7L50 SER C  176  UNP  A0A0C4DFN LEU   186 CONFLICT
SEQADV 7L50 MET D  -16  UNP  A0A0C4DFN           INITIATING METHIONINE
SEQADV 7L50 HIS D  -15  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS D  -14  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS D  -13  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS D  -12  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS D  -11  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 HIS D  -10  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLY D   -9  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 SER D   -8  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLU D   -7  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 ASN D   -6  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 LEU D   -5  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 TYR D   -4  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 PHE D   -3  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLN D   -2  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 GLY D   -1  UNP  A0A0C4DFN           EXPRESSION TAG
SEQADV 7L50 ALA D   36  UNP  A0A0C4DFN LYS    46 CONFLICT
SEQADV 7L50 SER D  169  UNP  A0A0C4DFN LEU   179 CONFLICT
SEQADV 7L50 SER D  176  UNP  A0A0C4DFN LEU   186 CONFLICT
SEQRES   1 A  320  MET HIS HIS HIS HIS HIS HIS GLY SER GLU ASN LEU TYR
SEQRES   2 A  320  PHE GLN GLY SER MET PRO GLU GLU SER SER PRO ARG ARG
SEQRES   3 A  320  THR PRO GLN SER ILE PRO TYR GLN ASP LEU PRO HIS LEU
SEQRES   4 A  320  VAL ASN ALA ASP GLY GLN TYR LEU PHE CYS ARG TYR TRP
SEQRES   5 A  320  ALA PRO THR GLY THR PRO LYS ALA LEU ILE PHE VAL SER
SEQRES   6 A  320  HIS GLY ALA GLY GLU HIS SER GLY ARG TYR GLU GLU LEU
SEQRES   7 A  320  ALA ARG MET LEU MET GLY LEU ASP LEU LEU VAL PHE ALA
SEQRES   8 A  320  HIS ASP HIS VAL GLY HIS GLY GLN SER GLU GLY GLU ARG
SEQRES   9 A  320  MET VAL VAL SER ASP PHE HIS VAL PHE VAL ARG ASP VAL
SEQRES  10 A  320  LEU GLN HIS VAL ASP SER MET GLN LYS ASP TYR PRO GLY
SEQRES  11 A  320  LEU PRO VAL PHE LEU LEU GLY HIS SER MET GLY GLY ALA
SEQRES  12 A  320  ILE ALA ILE LEU THR ALA ALA GLU ARG PRO GLY HIS PHE
SEQRES  13 A  320  ALA GLY MET VAL LEU ILE SER PRO LEU VAL LEU ALA ASN
SEQRES  14 A  320  PRO GLU SER ALA THR THR PHE LYS VAL LEU ALA ALA LYS
SEQRES  15 A  320  VAL LEU ASN SER VAL LEU PRO ASN LEU SER SER GLY PRO
SEQRES  16 A  320  ILE ASP SER SER VAL LEU SER ARG ASN LYS THR GLU VAL
SEQRES  17 A  320  ASP ILE TYR ASN SER ASP PRO LEU ILE CYS ARG ALA GLY
SEQRES  18 A  320  LEU LYS VAL CYS PHE GLY ILE GLN LEU LEU ASN ALA VAL
SEQRES  19 A  320  SER ARG VAL GLU ARG ALA LEU PRO LYS LEU THR VAL PRO
SEQRES  20 A  320  PHE LEU LEU LEU GLN GLY SER ALA ASP ARG LEU CYS ASP
SEQRES  21 A  320  SER LYS GLY ALA TYR LEU LEU MET GLU LEU ALA LYS SER
SEQRES  22 A  320  GLN ASP LYS THR LEU LYS ILE TYR GLU GLY ALA TYR HIS
SEQRES  23 A  320  VAL LEU HIS LYS GLU LEU PRO GLU VAL THR ASN SER VAL
SEQRES  24 A  320  PHE HIS GLU ILE ASN MET TRP VAL SER GLN ARG THR ALA
SEQRES  25 A  320  THR ALA GLY THR ALA SER PRO PRO
SEQRES   1 B  320  MET HIS HIS HIS HIS HIS HIS GLY SER GLU ASN LEU TYR
SEQRES   2 B  320  PHE GLN GLY SER MET PRO GLU GLU SER SER PRO ARG ARG
SEQRES   3 B  320  THR PRO GLN SER ILE PRO TYR GLN ASP LEU PRO HIS LEU
SEQRES   4 B  320  VAL ASN ALA ASP GLY GLN TYR LEU PHE CYS ARG TYR TRP
SEQRES   5 B  320  ALA PRO THR GLY THR PRO LYS ALA LEU ILE PHE VAL SER
SEQRES   6 B  320  HIS GLY ALA GLY GLU HIS SER GLY ARG TYR GLU GLU LEU
SEQRES   7 B  320  ALA ARG MET LEU MET GLY LEU ASP LEU LEU VAL PHE ALA
SEQRES   8 B  320  HIS ASP HIS VAL GLY HIS GLY GLN SER GLU GLY GLU ARG
SEQRES   9 B  320  MET VAL VAL SER ASP PHE HIS VAL PHE VAL ARG ASP VAL
SEQRES  10 B  320  LEU GLN HIS VAL ASP SER MET GLN LYS ASP TYR PRO GLY
SEQRES  11 B  320  LEU PRO VAL PHE LEU LEU GLY HIS SER MET GLY GLY ALA
SEQRES  12 B  320  ILE ALA ILE LEU THR ALA ALA GLU ARG PRO GLY HIS PHE
SEQRES  13 B  320  ALA GLY MET VAL LEU ILE SER PRO LEU VAL LEU ALA ASN
SEQRES  14 B  320  PRO GLU SER ALA THR THR PHE LYS VAL LEU ALA ALA LYS
SEQRES  15 B  320  VAL LEU ASN SER VAL LEU PRO ASN LEU SER SER GLY PRO
SEQRES  16 B  320  ILE ASP SER SER VAL LEU SER ARG ASN LYS THR GLU VAL
SEQRES  17 B  320  ASP ILE TYR ASN SER ASP PRO LEU ILE CYS ARG ALA GLY
SEQRES  18 B  320  LEU LYS VAL CYS PHE GLY ILE GLN LEU LEU ASN ALA VAL
SEQRES  19 B  320  SER ARG VAL GLU ARG ALA LEU PRO LYS LEU THR VAL PRO
SEQRES  20 B  320  PHE LEU LEU LEU GLN GLY SER ALA ASP ARG LEU CYS ASP
SEQRES  21 B  320  SER LYS GLY ALA TYR LEU LEU MET GLU LEU ALA LYS SER
SEQRES  22 B  320  GLN ASP LYS THR LEU LYS ILE TYR GLU GLY ALA TYR HIS
SEQRES  23 B  320  VAL LEU HIS LYS GLU LEU PRO GLU VAL THR ASN SER VAL
SEQRES  24 B  320  PHE HIS GLU ILE ASN MET TRP VAL SER GLN ARG THR ALA
SEQRES  25 B  320  THR ALA GLY THR ALA SER PRO PRO
SEQRES   1 C  320  MET HIS HIS HIS HIS HIS HIS GLY SER GLU ASN LEU TYR
SEQRES   2 C  320  PHE GLN GLY SER MET PRO GLU GLU SER SER PRO ARG ARG
SEQRES   3 C  320  THR PRO GLN SER ILE PRO TYR GLN ASP LEU PRO HIS LEU
SEQRES   4 C  320  VAL ASN ALA ASP GLY GLN TYR LEU PHE CYS ARG TYR TRP
SEQRES   5 C  320  ALA PRO THR GLY THR PRO LYS ALA LEU ILE PHE VAL SER
SEQRES   6 C  320  HIS GLY ALA GLY GLU HIS SER GLY ARG TYR GLU GLU LEU
SEQRES   7 C  320  ALA ARG MET LEU MET GLY LEU ASP LEU LEU VAL PHE ALA
SEQRES   8 C  320  HIS ASP HIS VAL GLY HIS GLY GLN SER GLU GLY GLU ARG
SEQRES   9 C  320  MET VAL VAL SER ASP PHE HIS VAL PHE VAL ARG ASP VAL
SEQRES  10 C  320  LEU GLN HIS VAL ASP SER MET GLN LYS ASP TYR PRO GLY
SEQRES  11 C  320  LEU PRO VAL PHE LEU LEU GLY HIS SER MET GLY GLY ALA
SEQRES  12 C  320  ILE ALA ILE LEU THR ALA ALA GLU ARG PRO GLY HIS PHE
SEQRES  13 C  320  ALA GLY MET VAL LEU ILE SER PRO LEU VAL LEU ALA ASN
SEQRES  14 C  320  PRO GLU SER ALA THR THR PHE LYS VAL LEU ALA ALA LYS
SEQRES  15 C  320  VAL LEU ASN SER VAL LEU PRO ASN LEU SER SER GLY PRO
SEQRES  16 C  320  ILE ASP SER SER VAL LEU SER ARG ASN LYS THR GLU VAL
SEQRES  17 C  320  ASP ILE TYR ASN SER ASP PRO LEU ILE CYS ARG ALA GLY
SEQRES  18 C  320  LEU LYS VAL CYS PHE GLY ILE GLN LEU LEU ASN ALA VAL
SEQRES  19 C  320  SER ARG VAL GLU ARG ALA LEU PRO LYS LEU THR VAL PRO
SEQRES  20 C  320  PHE LEU LEU LEU GLN GLY SER ALA ASP ARG LEU CYS ASP
SEQRES  21 C  320  SER LYS GLY ALA TYR LEU LEU MET GLU LEU ALA LYS SER
SEQRES  22 C  320  GLN ASP LYS THR LEU LYS ILE TYR GLU GLY ALA TYR HIS
SEQRES  23 C  320  VAL LEU HIS LYS GLU LEU PRO GLU VAL THR ASN SER VAL
SEQRES  24 C  320  PHE HIS GLU ILE ASN MET TRP VAL SER GLN ARG THR ALA
SEQRES  25 C  320  THR ALA GLY THR ALA SER PRO PRO
SEQRES   1 D  320  MET HIS HIS HIS HIS HIS HIS GLY SER GLU ASN LEU TYR
SEQRES   2 D  320  PHE GLN GLY SER MET PRO GLU GLU SER SER PRO ARG ARG
SEQRES   3 D  320  THR PRO GLN SER ILE PRO TYR GLN ASP LEU PRO HIS LEU
SEQRES   4 D  320  VAL ASN ALA ASP GLY GLN TYR LEU PHE CYS ARG TYR TRP
SEQRES   5 D  320  ALA PRO THR GLY THR PRO LYS ALA LEU ILE PHE VAL SER
SEQRES   6 D  320  HIS GLY ALA GLY GLU HIS SER GLY ARG TYR GLU GLU LEU
SEQRES   7 D  320  ALA ARG MET LEU MET GLY LEU ASP LEU LEU VAL PHE ALA
SEQRES   8 D  320  HIS ASP HIS VAL GLY HIS GLY GLN SER GLU GLY GLU ARG
SEQRES   9 D  320  MET VAL VAL SER ASP PHE HIS VAL PHE VAL ARG ASP VAL
SEQRES  10 D  320  LEU GLN HIS VAL ASP SER MET GLN LYS ASP TYR PRO GLY
SEQRES  11 D  320  LEU PRO VAL PHE LEU LEU GLY HIS SER MET GLY GLY ALA
SEQRES  12 D  320  ILE ALA ILE LEU THR ALA ALA GLU ARG PRO GLY HIS PHE
SEQRES  13 D  320  ALA GLY MET VAL LEU ILE SER PRO LEU VAL LEU ALA ASN
SEQRES  14 D  320  PRO GLU SER ALA THR THR PHE LYS VAL LEU ALA ALA LYS
SEQRES  15 D  320  VAL LEU ASN SER VAL LEU PRO ASN LEU SER SER GLY PRO
SEQRES  16 D  320  ILE ASP SER SER VAL LEU SER ARG ASN LYS THR GLU VAL
SEQRES  17 D  320  ASP ILE TYR ASN SER ASP PRO LEU ILE CYS ARG ALA GLY
SEQRES  18 D  320  LEU LYS VAL CYS PHE GLY ILE GLN LEU LEU ASN ALA VAL
SEQRES  19 D  320  SER ARG VAL GLU ARG ALA LEU PRO LYS LEU THR VAL PRO
SEQRES  20 D  320  PHE LEU LEU LEU GLN GLY SER ALA ASP ARG LEU CYS ASP
SEQRES  21 D  320  SER LYS GLY ALA TYR LEU LEU MET GLU LEU ALA LYS SER
SEQRES  22 D  320  GLN ASP LYS THR LEU LYS ILE TYR GLU GLY ALA TYR HIS
SEQRES  23 D  320  VAL LEU HIS LYS GLU LEU PRO GLU VAL THR ASN SER VAL
SEQRES  24 D  320  PHE HIS GLU ILE ASN MET TRP VAL SER GLN ARG THR ALA
SEQRES  25 D  320  THR ALA GLY THR ALA SER PRO PRO
HET    ACT  A 601       4
HET    ACT  A 602       4
HET    XOM  A 603      25
HET    ACT  B 601       4
HET    ACT  B 602       4
HET    XOM  B 603      25
HET    ACT  C 601       4
HET    ACT  C 602       4
HET    XOM  C 603      25
HET    ACT  D 601       4
HET    ACT  D 602       4
HET    XOM  D 603      25
HETNAM     ACT ACETATE ION
HETNAM     XOM (2S,4R)-2-{3-[(3-CHLORO-4-METHYLPHENYL)
HETNAM   2 XOM  METHOXY]AZETIDINE-1-CARBONYL}-7-OXA-5-
HETNAM   3 XOM  AZASPIRO[3.4]OCTAN-6-ONE
FORMUL   5  ACT    8(C2 H3 O2 1-)
FORMUL   7  XOM    4(C18 H21 CL N2 O4)
FORMUL  17  HOH   *157(H2 O)
HELIX    1 AA1 PRO A   15  LEU A   19  5                                   5
HELIX    2 AA2 HIS A   54  ARG A   57  5                                   4
HELIX    3 AA3 TYR A   58  LEU A   68  1                                  11
HELIX    4 AA4 PHE A   93  TYR A  111  1                                  19
HELIX    5 AA5 SER A  122  GLU A  134  1                                  13
HELIX    6 AA6 ASN A  152  LYS A  165  1                                  14
HELIX    7 AA7 ASP A  180  SER A  185  5                                   6
HELIX    8 AA8 ASN A  187  ASP A  197  1                                  11
HELIX    9 AA9 LYS A  206  LEU A  224  1                                  19
HELIX   10 AB1 PRO A  225  LEU A  227  5                                   3
HELIX   11 AB2 ASP A  243  ALA A  254  1                                  12
HELIX   12 AB3 VAL A  270  GLU A  274  5                                   5
HELIX   13 AB4 LEU A  275  ARG A  293  1                                  19
HELIX   14 AB5 PRO B   15  LEU B   19  5                                   5
HELIX   15 AB6 HIS B   54  ARG B   57  5                                   4
HELIX   16 AB7 TYR B   58  LEU B   68  1                                  11
HELIX   17 AB8 PHE B   93  TYR B  111  1                                  19
HELIX   18 AB9 SER B  122  ARG B  135  1                                  14
HELIX   19 AC1 ASN B  152  VAL B  166  1                                  15
HELIX   20 AC2 ASP B  180  LEU B  184  5                                   5
HELIX   21 AC3 ASN B  187  ASP B  197  1                                  11
HELIX   22 AC4 LYS B  206  LEU B  224  1                                  19
HELIX   23 AC5 PRO B  225  LEU B  227  5                                   3
HELIX   24 AC6 SER B  244  ALA B  254  1                                  11
HELIX   25 AC7 VAL B  270  GLU B  274  5                                   5
HELIX   26 AC8 LEU B  275  GLN B  292  1                                  18
HELIX   27 AC9 PRO C   15  LEU C   19  5                                   5
HELIX   28 AD1 HIS C   54  ARG C   57  5                                   4
HELIX   29 AD2 TYR C   58  GLY C   67  1                                  10
HELIX   30 AD3 PHE C   93  TYR C  111  1                                  19
HELIX   31 AD4 SER C  122  ARG C  135  1                                  14
HELIX   32 AD5 ASN C  152  LYS C  165  1                                  14
HELIX   33 AD6 ASP C  180  SER C  185  5                                   6
HELIX   34 AD7 ASN C  187  ASN C  195  1                                   9
HELIX   35 AD8 LYS C  206  LEU C  224  1                                  19
HELIX   36 AD9 PRO C  225  LEU C  227  5                                   3
HELIX   37 AE1 SER C  244  ALA C  254  1                                  11
HELIX   38 AE2 VAL C  270  GLU C  274  5                                   5
HELIX   39 AE3 LEU C  275  ARG C  293  1                                  19
HELIX   40 AE4 PRO D   15  LEU D   19  5                                   5
HELIX   41 AE5 HIS D   54  ARG D   57  5                                   4
HELIX   42 AE6 TYR D   58  GLY D   67  1                                  10
HELIX   43 AE7 PHE D   93  TYR D  111  1                                  19
HELIX   44 AE8 SER D  122  ARG D  135  1                                  14
HELIX   45 AE9 ASN D  152  VAL D  166  1                                  15
HELIX   46 AF1 ASP D  180  LEU D  184  5                                   5
HELIX   47 AF2 ASN D  187  ASN D  195  1                                   9
HELIX   48 AF3 LYS D  206  LEU D  224  1                                  19
HELIX   49 AF4 PRO D  225  LEU D  227  5                                   3
HELIX   50 AF5 ASP D  243  ALA D  254  1                                  12
HELIX   51 AF6 VAL D  270  GLU D  274  5                                   5
HELIX   52 AF7 LEU D  275  ARG D  293  1                                  19
SHEET    1 AA1 8 HIS A  21  VAL A  23  0
SHEET    2 AA1 8 TYR A  29  TRP A  35 -1  O  LEU A  30   N  LEU A  22
SHEET    3 AA1 8 LEU A  71  HIS A  75 -1  O  VAL A  72   N  TRP A  35
SHEET    4 AA1 8 LEU A  44  SER A  48  1  N  ILE A  45   O  LEU A  71
SHEET    5 AA1 8 VAL A 116  HIS A 121  1  O  LEU A 119   N  SER A  48
SHEET    6 AA1 8 GLY A 141  PRO A 147  1  O  VAL A 143   N  LEU A 118
SHEET    7 AA1 8 PHE A 231  GLY A 236  1  O  LEU A 234   N  SER A 146
SHEET    8 AA1 8 LYS A 259  TYR A 264  1  O  LYS A 262   N  LEU A 233
SHEET    1 AA2 8 HIS B  21  VAL B  23  0
SHEET    2 AA2 8 TYR B  29  TRP B  35 -1  O  LEU B  30   N  LEU B  22
SHEET    3 AA2 8 LEU B  70  HIS B  75 -1  O  ALA B  74   N  ARG B  33
SHEET    4 AA2 8 ALA B  43  SER B  48  1  N  ALA B  43   O  LEU B  71
SHEET    5 AA2 8 VAL B 116  HIS B 121  1  O  LEU B 119   N  SER B  48
SHEET    6 AA2 8 GLY B 141  ILE B 145  1  O  VAL B 143   N  LEU B 118
SHEET    7 AA2 8 PHE B 231  GLY B 236  1  O  LEU B 232   N  LEU B 144
SHEET    8 AA2 8 LYS B 259  TYR B 264  1  O  TYR B 264   N  GLN B 235
SHEET    1 AA316 HIS C  21  VAL C  23  0
SHEET    2 AA316 TYR C  29  TRP C  35 -1  O  LEU C  30   N  LEU C  22
SHEET    3 AA316 LEU C  70  HIS C  75 -1  O  VAL C  72   N  TRP C  35
SHEET    4 AA316 ALA C  43  SER C  48  1  N  ALA C  43   O  LEU C  71
SHEET    5 AA316 VAL C 116  HIS C 121  1  O  PHE C 117   N  PHE C  46
SHEET    6 AA316 GLY C 141  ILE C 145  1  O  VAL C 143   N  LEU C 118
SHEET    7 AA316 PHE C 231  GLY C 236  1  O  LEU C 234   N  LEU C 144
SHEET    8 AA316 LYS C 259  TYR C 264  1  O  TYR C 264   N  GLN C 235
SHEET    9 AA316 LYS D 259  TYR D 264 -1  O  LEU D 261   N  LEU C 261
SHEET   10 AA316 PHE D 231  GLY D 236  1  N  GLN D 235   O  TYR D 264
SHEET   11 AA316 GLY D 141  PRO D 147  1  N  SER D 146   O  LEU D 234
SHEET   12 AA316 VAL D 116  HIS D 121  1  N  LEU D 118   O  VAL D 143
SHEET   13 AA316 ALA D  43  SER D  48  1  N  PHE D  46   O  LEU D 119
SHEET   14 AA316 LEU D  70  HIS D  75  1  O  LEU D  71   N  ALA D  43
SHEET   15 AA316 TYR D  29  TRP D  35 -1  N  TRP D  35   O  VAL D  72
SHEET   16 AA316 HIS D  21  VAL D  23 -1  N  LEU D  22   O  LEU D  30
CRYST1   62.894  127.168  119.675  90.00  73.67  90.00 P 1 21 1      8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.015900  0.000000 -0.004658        0.00000
SCALE2      0.000000  0.007864  0.000000        0.00000
SCALE3      0.000000  0.000000  0.008707        0.00000
TER    2206      ALA A 295
TER    4445      ALA B 295
TER    6659      ALA C 295
TER    8868      THR D 294
MASTER      530    0   12   52   32    0    0    6 9153    4  132  100
END