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HEADER HYDROLASE 04-JUN-21 7OR4
TITLE CRYSTAL STRUCTURE OF DPP8 IN COMPLEX WITH A 4-OXO-B-LACTAM BASED
TITLE 2 INHIBITOR, B142
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: DIPEPTIDYL PEPTIDASE 8;
COMPND 3 CHAIN: B, A;
COMPND 4 SYNONYM: DP8,DIPEPTIDYL PEPTIDASE IV-RELATED PROTEIN 1,DPRP-1,
COMPND 5 DIPEPTIDYL PEPTIDASE VIII,DPP VIII,PROLYL DIPEPTIDASE DPP8;
COMPND 6 EC: 3.4.14.5;
COMPND 7 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 GENE: DPP8, DPRP1, MSTP097, MSTP135, MSTP141;
SOURCE 6 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;
SOURCE 7 EXPRESSION_SYSTEM_TAXID: 7108
KEYWDS DPP8, DPP9, 4-OXO-B-LACTAM, HYDROLASE
EXPDTA X-RAY DIFFRACTION
AUTHOR B.ROSS,R.HUBER
REVDAT 1 07-JUL-21 7OR4 0
JRNL AUTH L.FEHR,L.A.R.CARVALHO,B.H.ROSS,K.LUM,A.C.VIEIRA,
JRNL AUTH 2 R.KIEFERSAUER,R.GEISS-FRIEDLANDER,M.KAISER,T.RODRIGUES,
JRNL AUTH 3 S.D.LUCAS,B.F.CRAVATT,R.HUBER,R.MOREIRA
JRNL TITL DISCOVERY AND DEVELOPMENT OF 4-OXO-BETA-LACTAMS AS NOVEL
JRNL TITL 2 INHIBITORS OF DIPEPTIDYL PEPTIDASES 8 AND 9
JRNL REF TO BE PUBLISHED
JRNL REFN
REMARK 2
REMARK 2 RESOLUTION. 2.44 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : REFMAC 5.8.0267
REMARK 3 AUTHORS : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK 3 : NICHOLLS,WINN,LONG,VAGIN
REMARK 3
REMARK 3 REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK 3
REMARK 3 DATA USED IN REFINEMENT.
REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS) : 2.44
REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS) : 49.74
REMARK 3 DATA CUTOFF (SIGMA(F)) : 0.000
REMARK 3 COMPLETENESS FOR RANGE (%) : 100.0
REMARK 3 NUMBER OF REFLECTIONS : 119719
REMARK 3
REMARK 3 FIT TO DATA USED IN REFINEMENT.
REMARK 3 CROSS-VALIDATION METHOD : THROUGHOUT
REMARK 3 FREE R VALUE TEST SET SELECTION : RANDOM
REMARK 3 R VALUE (WORKING + TEST SET) : 0.217
REMARK 3 R VALUE (WORKING SET) : 0.215
REMARK 3 FREE R VALUE : 0.253
REMARK 3 FREE R VALUE TEST SET SIZE (%) : 5.000
REMARK 3 FREE R VALUE TEST SET COUNT : 6301
REMARK 3
REMARK 3 FIT IN THE HIGHEST RESOLUTION BIN.
REMARK 3 TOTAL NUMBER OF BINS USED : 20
REMARK 3 BIN RESOLUTION RANGE HIGH (A) : 2.44
REMARK 3 BIN RESOLUTION RANGE LOW (A) : 2.50
REMARK 3 REFLECTION IN BIN (WORKING SET) : 8832
REMARK 3 BIN COMPLETENESS (WORKING+TEST) (%) : 99.97
REMARK 3 BIN R VALUE (WORKING SET) : 0.3790
REMARK 3 BIN FREE R VALUE SET COUNT : 464
REMARK 3 BIN FREE R VALUE : 0.4220
REMARK 3
REMARK 3 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK 3 PROTEIN ATOMS : 13151
REMARK 3 NUCLEIC ACID ATOMS : 0
REMARK 3 HETEROGEN ATOMS : 82
REMARK 3 SOLVENT ATOMS : 168
REMARK 3
REMARK 3 B VALUES.
REMARK 3 FROM WILSON PLOT (A**2) : 67.35
REMARK 3 MEAN B VALUE (OVERALL, A**2) : 64.50
REMARK 3 OVERALL ANISOTROPIC B VALUE.
REMARK 3 B11 (A**2) : 1.00000
REMARK 3 B22 (A**2) : 1.00000
REMARK 3 B33 (A**2) : -3.26000
REMARK 3 B12 (A**2) : 0.50000
REMARK 3 B13 (A**2) : 0.00000
REMARK 3 B23 (A**2) : 0.00000
REMARK 3
REMARK 3 ESTIMATED OVERALL COORDINATE ERROR.
REMARK 3 ESU BASED ON R VALUE (A): 0.240
REMARK 3 ESU BASED ON FREE R VALUE (A): 0.210
REMARK 3 ESU BASED ON MAXIMUM LIKELIHOOD (A): 0.186
REMARK 3 ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 8.349
REMARK 3
REMARK 3 CORRELATION COEFFICIENTS.
REMARK 3 CORRELATION COEFFICIENT FO-FC : 0.942
REMARK 3 CORRELATION COEFFICIENT FO-FC FREE : 0.924
REMARK 3
REMARK 3 RMS DEVIATIONS FROM IDEAL VALUES COUNT RMS WEIGHT
REMARK 3 BOND LENGTHS REFINED ATOMS (A): 13612 ; 0.008 ; 0.013
REMARK 3 BOND LENGTHS OTHERS (A): 12539 ; 0.002 ; 0.017
REMARK 3 BOND ANGLES REFINED ATOMS (DEGREES): 18476 ; 1.579 ; 1.653
REMARK 3 BOND ANGLES OTHERS (DEGREES): 28946 ; 1.242 ; 1.583
REMARK 3 TORSION ANGLES, PERIOD 1 (DEGREES): 1612 ; 8.274 ; 5.000
REMARK 3 TORSION ANGLES, PERIOD 2 (DEGREES): 742 ;33.502 ;22.116
REMARK 3 TORSION ANGLES, PERIOD 3 (DEGREES): 2254 ;17.633 ;15.000
REMARK 3 TORSION ANGLES, PERIOD 4 (DEGREES): 82 ;19.074 ;15.000
REMARK 3 CHIRAL-CENTER RESTRAINTS (A**3): 1713 ; 0.071 ; 0.200
REMARK 3 GENERAL PLANES REFINED ATOMS (A): 15269 ; 0.007 ; 0.020
REMARK 3 GENERAL PLANES OTHERS (A): 3207 ; 0.002 ; 0.020
REMARK 3 NON-BONDED CONTACTS REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 NON-BONDED CONTACTS OTHERS (A): NULL ; NULL ; NULL
REMARK 3 NON-BONDED TORSION REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 NON-BONDED TORSION OTHERS (A): NULL ; NULL ; NULL
REMARK 3 H-BOND (X...Y) REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 H-BOND (X...Y) OTHERS (A): NULL ; NULL ; NULL
REMARK 3 POTENTIAL METAL-ION REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 POTENTIAL METAL-ION OTHERS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY VDW REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY VDW OTHERS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY H-BOND REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY H-BOND OTHERS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY METAL-ION REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY METAL-ION OTHERS (A): NULL ; NULL ; NULL
REMARK 3
REMARK 3 ISOTROPIC THERMAL FACTOR RESTRAINTS. COUNT RMS WEIGHT
REMARK 3 MAIN-CHAIN BOND REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 MAIN-CHAIN BOND OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 MAIN-CHAIN ANGLE REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 MAIN-CHAIN ANGLE OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SIDE-CHAIN BOND REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SIDE-CHAIN BOND OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SIDE-CHAIN ANGLE REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SIDE-CHAIN ANGLE OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 LONG RANGE B REFINED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 LONG RANGE B OTHER ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3
REMARK 3 ANISOTROPIC THERMAL FACTOR RESTRAINTS. COUNT RMS WEIGHT
REMARK 3 RIGID-BOND RESTRAINTS (A**2): NULL ; NULL ; NULL
REMARK 3 SPHERICITY; FREE ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SPHERICITY; BONDED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3
REMARK 3 NCS RESTRAINTS STATISTICS
REMARK 3 NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK 3
REMARK 3 TLS DETAILS
REMARK 3 NUMBER OF TLS GROUPS : NULL
REMARK 3
REMARK 3 BULK SOLVENT MODELLING.
REMARK 3 METHOD USED : MASK
REMARK 3 PARAMETERS FOR MASK CALCULATION
REMARK 3 VDW PROBE RADIUS : 1.20
REMARK 3 ION PROBE RADIUS : 0.80
REMARK 3 SHRINKAGE RADIUS : 0.80
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING
REMARK 3 POSITIONS U VALUES : REFINED INDIVIDUALLY
REMARK 4
REMARK 4 7OR4 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 23-JUN-21.
REMARK 100 THE DEPOSITION ID IS D_1292116219.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200 EXPERIMENT TYPE : X-RAY DIFFRACTION
REMARK 200 DATE OF DATA COLLECTION : 02-DEC-20
REMARK 200 TEMPERATURE (KELVIN) : 100
REMARK 200 PH : NULL
REMARK 200 NUMBER OF CRYSTALS USED : 1
REMARK 200
REMARK 200 SYNCHROTRON (Y/N) : Y
REMARK 200 RADIATION SOURCE : SLS
REMARK 200 BEAMLINE : X10SA
REMARK 200 X-RAY GENERATOR MODEL : NULL
REMARK 200 MONOCHROMATIC OR LAUE (M/L) : M
REMARK 200 WAVELENGTH OR RANGE (A) : 1.00
REMARK 200 MONOCHROMATOR : NULL
REMARK 200 OPTICS : NULL
REMARK 200
REMARK 200 DETECTOR TYPE : PIXEL
REMARK 200 DETECTOR MANUFACTURER : DECTRIS PILATUS 6M-F
REMARK 200 INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200 DATA SCALING SOFTWARE : XSCALE
REMARK 200
REMARK 200 NUMBER OF UNIQUE REFLECTIONS : 126021
REMARK 200 RESOLUTION RANGE HIGH (A) : 2.440
REMARK 200 RESOLUTION RANGE LOW (A) : 49.740
REMARK 200 REJECTION CRITERIA (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200 COMPLETENESS FOR RANGE (%) : 100.0
REMARK 200 DATA REDUNDANCY : 13.18
REMARK 200 R MERGE (I) : 0.07300
REMARK 200 R SYM (I) : NULL
REMARK 200 FOR THE DATA SET : 22.9600
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.44
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 2.50
REMARK 200 COMPLETENESS FOR SHELL (%) : 100.0
REMARK 200 DATA REDUNDANCY IN SHELL : 12.20
REMARK 200 R MERGE FOR SHELL (I) : 1.25500
REMARK 200 R SYM FOR SHELL (I) : NULL
REMARK 200 FOR SHELL : 2.060
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: 6EOO
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS (%): 70.68
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 4.20
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.46 NA CITRATE, VAPOR DIFFUSION,
REMARK 280 HANGING DROP, TEMPERATURE 277K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 61
REMARK 290
REMARK 290 SYMOP SYMMETRY
REMARK 290 NNNMMM OPERATOR
REMARK 290 1555 X,Y,Z
REMARK 290 2555 -Y,X-Y,Z+1/3
REMARK 290 3555 -X+Y,-X,Z+2/3
REMARK 290 4555 -X,-Y,Z+1/2
REMARK 290 5555 Y,-X+Y,Z+5/6
REMARK 290 6555 X-Y,X,Z+1/6
REMARK 290
REMARK 290 WHERE NNN -> OPERATOR NUMBER
REMARK 290 MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000
REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000
REMARK 290 SMTRY1 2 -0.500000 -0.866025 0.000000 0.00000
REMARK 290 SMTRY2 2 0.866025 -0.500000 0.000000 0.00000
REMARK 290 SMTRY3 2 0.000000 0.000000 1.000000 89.79600
REMARK 290 SMTRY1 3 -0.500000 0.866025 0.000000 0.00000
REMARK 290 SMTRY2 3 -0.866025 -0.500000 0.000000 0.00000
REMARK 290 SMTRY3 3 0.000000 0.000000 1.000000 179.59200
REMARK 290 SMTRY1 4 -1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 4 0.000000 -1.000000 0.000000 0.00000
REMARK 290 SMTRY3 4 0.000000 0.000000 1.000000 134.69400
REMARK 290 SMTRY1 5 0.500000 0.866025 0.000000 0.00000
REMARK 290 SMTRY2 5 -0.866025 0.500000 0.000000 0.00000
REMARK 290 SMTRY3 5 0.000000 0.000000 1.000000 224.49000
REMARK 290 SMTRY1 6 0.500000 -0.866025 0.000000 0.00000
REMARK 290 SMTRY2 6 0.866025 0.500000 0.000000 0.00000
REMARK 290 SMTRY3 6 0.000000 0.000000 1.000000 44.89800
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 2810 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 65430 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -14.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B, A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 MET B 1
REMARK 465 TRP B 2
REMARK 465 LYS B 3
REMARK 465 ARG B 4
REMARK 465 SER B 5
REMARK 465 GLU B 6
REMARK 465 GLN B 7
REMARK 465 MET B 8
REMARK 465 LYS B 9
REMARK 465 ILE B 10
REMARK 465 LYS B 11
REMARK 465 SER B 12
REMARK 465 GLY B 13
REMARK 465 LYS B 14
REMARK 465 CYS B 15
REMARK 465 ASN B 16
REMARK 465 MET B 17
REMARK 465 ALA B 18
REMARK 465 ALA B 19
REMARK 465 ALA B 20
REMARK 465 MET B 21
REMARK 465 GLU B 22
REMARK 465 THR B 23
REMARK 465 GLU B 24
REMARK 465 GLN B 25
REMARK 465 LEU B 26
REMARK 465 GLY B 27
REMARK 465 VAL B 28
REMARK 465 GLU B 29
REMARK 465 ILE B 30
REMARK 465 PHE B 31
REMARK 465 GLU B 32
REMARK 465 THR B 33
REMARK 465 ALA B 34
REMARK 465 ASP B 35
REMARK 465 CYS B 36
REMARK 465 GLU B 37
REMARK 465 GLU B 38
REMARK 465 ASN B 39
REMARK 465 ILE B 40
REMARK 465 GLU B 41
REMARK 465 SER B 42
REMARK 465 GLN B 43
REMARK 465 ASP B 44
REMARK 465 ARG B 45
REMARK 465 PRO B 46
REMARK 465 LYS B 47
REMARK 465 GLY B 106
REMARK 465 GLU B 107
REMARK 465 GLN B 140
REMARK 465 ALA B 141
REMARK 465 THR B 142
REMARK 465 LEU B 143
REMARK 465 ASP B 144
REMARK 465 TYR B 145
REMARK 465 GLY B 146
REMARK 465 MET B 147
REMARK 465 TYR B 148
REMARK 465 SER B 149
REMARK 465 ARG B 150
REMARK 465 GLU B 151
REMARK 465 GLU B 152
REMARK 465 GLU B 153
REMARK 465 LEU B 154
REMARK 465 LEU B 155
REMARK 465 ARG B 156
REMARK 465 GLU B 157
REMARK 465 ARG B 158
REMARK 465 LYS B 159
REMARK 465 ARG B 160
REMARK 465 ILE B 161
REMARK 465 GLY B 162
REMARK 465 THR B 163
REMARK 465 SER B 318
REMARK 465 PRO B 319
REMARK 465 MET B 320
REMARK 465 LEU B 321
REMARK 465 GLU B 322
REMARK 465 THR B 323
REMARK 465 ARG B 324
REMARK 465 ARG B 325
REMARK 465 ALA B 326
REMARK 465 ILE B 898
REMARK 465 MET A 1
REMARK 465 TRP A 2
REMARK 465 LYS A 3
REMARK 465 ARG A 4
REMARK 465 SER A 5
REMARK 465 GLU A 6
REMARK 465 GLN A 7
REMARK 465 MET A 8
REMARK 465 LYS A 9
REMARK 465 ILE A 10
REMARK 465 LYS A 11
REMARK 465 SER A 12
REMARK 465 GLY A 13
REMARK 465 LYS A 14
REMARK 465 CYS A 15
REMARK 465 ASN A 16
REMARK 465 MET A 17
REMARK 465 ALA A 18
REMARK 465 ALA A 19
REMARK 465 ALA A 20
REMARK 465 MET A 21
REMARK 465 GLU A 22
REMARK 465 THR A 23
REMARK 465 GLU A 24
REMARK 465 GLN A 25
REMARK 465 LEU A 26
REMARK 465 GLY A 27
REMARK 465 VAL A 28
REMARK 465 GLU A 29
REMARK 465 ILE A 30
REMARK 465 PHE A 31
REMARK 465 GLU A 32
REMARK 465 THR A 33
REMARK 465 ALA A 34
REMARK 465 ASP A 35
REMARK 465 CYS A 36
REMARK 465 GLU A 37
REMARK 465 GLU A 38
REMARK 465 ASN A 39
REMARK 465 ILE A 40
REMARK 465 GLU A 41
REMARK 465 SER A 42
REMARK 465 GLN A 43
REMARK 465 ASP A 44
REMARK 465 ARG A 45
REMARK 465 PRO A 46
REMARK 465 LYS A 47
REMARK 465 TYR A 71
REMARK 465 HIS A 72
REMARK 465 GLY A 73
REMARK 465 TYR A 74
REMARK 465 GLY A 106
REMARK 465 GLU A 107
REMARK 465 GLN A 140
REMARK 465 ALA A 141
REMARK 465 THR A 142
REMARK 465 LEU A 143
REMARK 465 ASP A 144
REMARK 465 TYR A 145
REMARK 465 GLY A 146
REMARK 465 MET A 147
REMARK 465 TYR A 148
REMARK 465 SER A 149
REMARK 465 ARG A 150
REMARK 465 GLU A 151
REMARK 465 GLU A 152
REMARK 465 GLU A 153
REMARK 465 LEU A 154
REMARK 465 LEU A 155
REMARK 465 ARG A 156
REMARK 465 GLU A 157
REMARK 465 ARG A 158
REMARK 465 LYS A 159
REMARK 465 ARG A 160
REMARK 465 ILE A 161
REMARK 465 GLY A 162
REMARK 465 THR A 163
REMARK 465 SER A 318
REMARK 465 PRO A 319
REMARK 465 MET A 320
REMARK 465 LEU A 321
REMARK 465 GLU A 322
REMARK 465 THR A 323
REMARK 465 ARG A 324
REMARK 465 ARG A 325
REMARK 465 ALA A 326
REMARK 465 GLU A 863
REMARK 465 ARG A 864
REMARK 465 HIS A 865
REMARK 465 SER A 866
REMARK 465 ILE A 867
REMARK 465 ARG A 868
REMARK 465 ILE A 898
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE
REMARK 500 CB CYS B 611 O HOH B 1051 2.05
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 HIS B 72 163.54 82.64
REMARK 500 TYR B 74 -7.67 87.76
REMARK 500 PRO B 80 132.35 -38.58
REMARK 500 ASN B 123 97.57 -67.41
REMARK 500 ASP B 224 103.72 -163.88
REMARK 500 SER B 233 56.59 40.00
REMARK 500 PHE B 277 -1.42 -141.40
REMARK 500 PHE B 372 67.36 -116.86
REMARK 500 ILE B 445 -97.47 -103.62
REMARK 500 PHE B 453 114.59 -163.37
REMARK 500 SER B 546 141.63 -177.83
REMARK 500 CYS B 581 30.01 74.50
REMARK 500 ALA B 618 145.63 -174.85
REMARK 500 TYR B 669 -72.76 -108.13
REMARK 500 TYR B 720 -2.53 77.04
REMARK 500 SER B 755 -121.30 61.28
REMARK 500 ARG B 768 52.48 -140.68
REMARK 500 ALA B 779 56.88 38.65
REMARK 500 ASN B 835 -64.52 -98.59
REMARK 500 GLU B 863 -11.85 90.73
REMARK 500 ARG B 864 -51.70 66.60
REMARK 500 SER B 866 -92.61 52.66
REMARK 500 LEU B 888 -50.69 -135.82
REMARK 500 MET A 76 159.62 73.71
REMARK 500 PRO A 91 -36.71 -38.94
REMARK 500 PRO A 332 84.80 -64.22
REMARK 500 PRO A 367 152.89 -49.07
REMARK 500 ASP A 417 -70.14 -62.13
REMARK 500 VAL A 429 113.61 -37.23
REMARK 500 ILE A 445 -95.25 -105.15
REMARK 500 PHE A 453 106.05 -164.92
REMARK 500 GLU A 511 84.64 -152.24
REMARK 500 ALA A 513 109.59 -56.12
REMARK 500 GLU A 518 57.29 -94.92
REMARK 500 ASP A 629 57.28 -143.72
REMARK 500 ASP A 655 49.78 35.69
REMARK 500 TYR A 669 -71.69 -105.11
REMARK 500 VAL A 684 -63.81 -94.19
REMARK 500 TYR A 720 -9.02 81.76
REMARK 500 SER A 755 -121.50 62.33
REMARK 500 ALA A 779 55.39 32.74
REMARK 500 LEU A 783 101.64 -160.40
REMARK 500 ASN A 802 41.08 -152.53
REMARK 500 GLN A 814 28.16 -152.39
REMARK 500 HIS A 837 150.24 -47.70
REMARK 500 LEU A 888 -54.08 -132.40
REMARK 500
REMARK 500 REMARK: NULL
DBREF 7OR4 B 1 898 UNP Q6V1X1 DPP8_HUMAN 1 898
DBREF 7OR4 A 1 898 UNP Q6V1X1 DPP8_HUMAN 1 898
SEQRES 1 B 898 MET TRP LYS ARG SER GLU GLN MET LYS ILE LYS SER GLY
SEQRES 2 B 898 LYS CYS ASN MET ALA ALA ALA MET GLU THR GLU GLN LEU
SEQRES 3 B 898 GLY VAL GLU ILE PHE GLU THR ALA ASP CYS GLU GLU ASN
SEQRES 4 B 898 ILE GLU SER GLN ASP ARG PRO LYS LEU GLU PRO PHE TYR
SEQRES 5 B 898 VAL GLU ARG TYR SER TRP SER GLN LEU LYS LYS LEU LEU
SEQRES 6 B 898 ALA ASP THR ARG LYS TYR HIS GLY TYR MET MET ALA LYS
SEQRES 7 B 898 ALA PRO HIS ASP PHE MET PHE VAL LYS ARG ASN ASP PRO
SEQRES 8 B 898 ASP GLY PRO HIS SER ASP ARG ILE TYR TYR LEU ALA MET
SEQRES 9 B 898 SER GLY GLU ASN ARG GLU ASN THR LEU PHE TYR SER GLU
SEQRES 10 B 898 ILE PRO LYS THR ILE ASN ARG ALA ALA VAL LEU MET LEU
SEQRES 11 B 898 SER TRP LYS PRO LEU LEU ASP LEU PHE GLN ALA THR LEU
SEQRES 12 B 898 ASP TYR GLY MET TYR SER ARG GLU GLU GLU LEU LEU ARG
SEQRES 13 B 898 GLU ARG LYS ARG ILE GLY THR VAL GLY ILE ALA SER TYR
SEQRES 14 B 898 ASP TYR HIS GLN GLY SER GLY THR PHE LEU PHE GLN ALA
SEQRES 15 B 898 GLY SER GLY ILE TYR HIS VAL LYS ASP GLY GLY PRO GLN
SEQRES 16 B 898 GLY PHE THR GLN GLN PRO LEU ARG PRO ASN LEU VAL GLU
SEQRES 17 B 898 THR SER CYS PRO ASN ILE ARG MET ASP PRO LYS LEU CYS
SEQRES 18 B 898 PRO ALA ASP PRO ASP TRP ILE ALA PHE ILE HIS SER ASN
SEQRES 19 B 898 ASP ILE TRP ILE SER ASN ILE VAL THR ARG GLU GLU ARG
SEQRES 20 B 898 ARG LEU THR TYR VAL HIS ASN GLU LEU ALA ASN MET GLU
SEQRES 21 B 898 GLU ASP ALA ARG SER ALA GLY VAL ALA THR PHE VAL LEU
SEQRES 22 B 898 GLN GLU GLU PHE ASP ARG TYR SER GLY TYR TRP TRP CYS
SEQRES 23 B 898 PRO LYS ALA GLU THR THR PRO SER GLY GLY LYS ILE LEU
SEQRES 24 B 898 ARG ILE LEU TYR GLU GLU ASN ASP GLU SER GLU VAL GLU
SEQRES 25 B 898 ILE ILE HIS VAL THR SER PRO MET LEU GLU THR ARG ARG
SEQRES 26 B 898 ALA ASP SER PHE ARG TYR PRO LYS THR GLY THR ALA ASN
SEQRES 27 B 898 PRO LYS VAL THR PHE LYS MET SER GLU ILE MET ILE ASP
SEQRES 28 B 898 ALA GLU GLY ARG ILE ILE ASP VAL ILE ASP LYS GLU LEU
SEQRES 29 B 898 ILE GLN PRO PHE GLU ILE LEU PHE GLU GLY VAL GLU TYR
SEQRES 30 B 898 ILE ALA ARG ALA GLY TRP THR PRO GLU GLY LYS TYR ALA
SEQRES 31 B 898 TRP SER ILE LEU LEU ASP ARG SER GLN THR ARG LEU GLN
SEQRES 32 B 898 ILE VAL LEU ILE SER PRO GLU LEU PHE ILE PRO VAL GLU
SEQRES 33 B 898 ASP ASP VAL MET GLU ARG GLN ARG LEU ILE GLU SER VAL
SEQRES 34 B 898 PRO ASP SER VAL THR PRO LEU ILE ILE TYR GLU GLU THR
SEQRES 35 B 898 THR ASP ILE TRP ILE ASN ILE HIS ASP ILE PHE HIS VAL
SEQRES 36 B 898 PHE PRO GLN SER HIS GLU GLU GLU ILE GLU PHE ILE PHE
SEQRES 37 B 898 ALA SER GLU CYS LYS THR GLY PHE ARG HIS LEU TYR LYS
SEQRES 38 B 898 ILE THR SER ILE LEU LYS GLU SER LYS TYR LYS ARG SER
SEQRES 39 B 898 SER GLY GLY LEU PRO ALA PRO SER ASP PHE LYS CYS PRO
SEQRES 40 B 898 ILE LYS GLU GLU ILE ALA ILE THR SER GLY GLU TRP GLU
SEQRES 41 B 898 VAL LEU GLY ARG HIS GLY SER ASN ILE GLN VAL ASP GLU
SEQRES 42 B 898 VAL ARG ARG LEU VAL TYR PHE GLU GLY THR LYS ASP SER
SEQRES 43 B 898 PRO LEU GLU HIS HIS LEU TYR VAL VAL SER TYR VAL ASN
SEQRES 44 B 898 PRO GLY GLU VAL THR ARG LEU THR ASP ARG GLY TYR SER
SEQRES 45 B 898 HIS SER CYS CYS ILE SER GLN HIS CYS ASP PHE PHE ILE
SEQRES 46 B 898 SER LYS TYR SER ASN GLN LYS ASN PRO HIS CYS VAL SER
SEQRES 47 B 898 LEU TYR LYS LEU SER SER PRO GLU ASP ASP PRO THR CYS
SEQRES 48 B 898 LYS THR LYS GLU PHE TRP ALA THR ILE LEU ASP SER ALA
SEQRES 49 B 898 GLY PRO LEU PRO ASP TYR THR PRO PRO GLU ILE PHE SER
SEQRES 50 B 898 PHE GLU SER THR THR GLY PHE THR LEU TYR GLY MET LEU
SEQRES 51 B 898 TYR LYS PRO HIS ASP LEU GLN PRO GLY LYS LYS TYR PRO
SEQRES 52 B 898 THR VAL LEU PHE ILE TYR GLY GLY PRO GLN VAL GLN LEU
SEQRES 53 B 898 VAL ASN ASN ARG PHE LYS GLY VAL LYS TYR PHE ARG LEU
SEQRES 54 B 898 ASN THR LEU ALA SER LEU GLY TYR VAL VAL VAL VAL ILE
SEQRES 55 B 898 ASP ASN ARG GLY SER CYS HIS ARG GLY LEU LYS PHE GLU
SEQRES 56 B 898 GLY ALA PHE LYS TYR LYS MET GLY GLN ILE GLU ILE ASP
SEQRES 57 B 898 ASP GLN VAL GLU GLY LEU GLN TYR LEU ALA SER ARG TYR
SEQRES 58 B 898 ASP PHE ILE ASP LEU ASP ARG VAL GLY ILE HIS GLY TRP
SEQRES 59 B 898 SER TYR GLY GLY TYR LEU SER LEU MET ALA LEU MET GLN
SEQRES 60 B 898 ARG SER ASP ILE PHE ARG VAL ALA ILE ALA GLY ALA PRO
SEQRES 61 B 898 VAL THR LEU TRP ILE PHE TYR ASP THR GLY TYR THR GLU
SEQRES 62 B 898 ARG TYR MET GLY HIS PRO ASP GLN ASN GLU GLN GLY TYR
SEQRES 63 B 898 TYR LEU GLY SER VAL ALA MET GLN ALA GLU LYS PHE PRO
SEQRES 64 B 898 SER GLU PRO ASN ARG LEU LEU LEU LEU HIS GLY PHE LEU
SEQRES 65 B 898 ASP GLU ASN VAL HIS PHE ALA HIS THR SER ILE LEU LEU
SEQRES 66 B 898 SER PHE LEU VAL ARG ALA GLY LYS PRO TYR ASP LEU GLN
SEQRES 67 B 898 ILE TYR PRO GLN GLU ARG HIS SER ILE ARG VAL PRO GLU
SEQRES 68 B 898 SER GLY GLU HIS TYR GLU LEU HIS LEU LEU HIS TYR LEU
SEQRES 69 B 898 GLN GLU ASN LEU GLY SER ARG ILE ALA ALA LEU LYS VAL
SEQRES 70 B 898 ILE
SEQRES 1 A 898 MET TRP LYS ARG SER GLU GLN MET LYS ILE LYS SER GLY
SEQRES 2 A 898 LYS CYS ASN MET ALA ALA ALA MET GLU THR GLU GLN LEU
SEQRES 3 A 898 GLY VAL GLU ILE PHE GLU THR ALA ASP CYS GLU GLU ASN
SEQRES 4 A 898 ILE GLU SER GLN ASP ARG PRO LYS LEU GLU PRO PHE TYR
SEQRES 5 A 898 VAL GLU ARG TYR SER TRP SER GLN LEU LYS LYS LEU LEU
SEQRES 6 A 898 ALA ASP THR ARG LYS TYR HIS GLY TYR MET MET ALA LYS
SEQRES 7 A 898 ALA PRO HIS ASP PHE MET PHE VAL LYS ARG ASN ASP PRO
SEQRES 8 A 898 ASP GLY PRO HIS SER ASP ARG ILE TYR TYR LEU ALA MET
SEQRES 9 A 898 SER GLY GLU ASN ARG GLU ASN THR LEU PHE TYR SER GLU
SEQRES 10 A 898 ILE PRO LYS THR ILE ASN ARG ALA ALA VAL LEU MET LEU
SEQRES 11 A 898 SER TRP LYS PRO LEU LEU ASP LEU PHE GLN ALA THR LEU
SEQRES 12 A 898 ASP TYR GLY MET TYR SER ARG GLU GLU GLU LEU LEU ARG
SEQRES 13 A 898 GLU ARG LYS ARG ILE GLY THR VAL GLY ILE ALA SER TYR
SEQRES 14 A 898 ASP TYR HIS GLN GLY SER GLY THR PHE LEU PHE GLN ALA
SEQRES 15 A 898 GLY SER GLY ILE TYR HIS VAL LYS ASP GLY GLY PRO GLN
SEQRES 16 A 898 GLY PHE THR GLN GLN PRO LEU ARG PRO ASN LEU VAL GLU
SEQRES 17 A 898 THR SER CYS PRO ASN ILE ARG MET ASP PRO LYS LEU CYS
SEQRES 18 A 898 PRO ALA ASP PRO ASP TRP ILE ALA PHE ILE HIS SER ASN
SEQRES 19 A 898 ASP ILE TRP ILE SER ASN ILE VAL THR ARG GLU GLU ARG
SEQRES 20 A 898 ARG LEU THR TYR VAL HIS ASN GLU LEU ALA ASN MET GLU
SEQRES 21 A 898 GLU ASP ALA ARG SER ALA GLY VAL ALA THR PHE VAL LEU
SEQRES 22 A 898 GLN GLU GLU PHE ASP ARG TYR SER GLY TYR TRP TRP CYS
SEQRES 23 A 898 PRO LYS ALA GLU THR THR PRO SER GLY GLY LYS ILE LEU
SEQRES 24 A 898 ARG ILE LEU TYR GLU GLU ASN ASP GLU SER GLU VAL GLU
SEQRES 25 A 898 ILE ILE HIS VAL THR SER PRO MET LEU GLU THR ARG ARG
SEQRES 26 A 898 ALA ASP SER PHE ARG TYR PRO LYS THR GLY THR ALA ASN
SEQRES 27 A 898 PRO LYS VAL THR PHE LYS MET SER GLU ILE MET ILE ASP
SEQRES 28 A 898 ALA GLU GLY ARG ILE ILE ASP VAL ILE ASP LYS GLU LEU
SEQRES 29 A 898 ILE GLN PRO PHE GLU ILE LEU PHE GLU GLY VAL GLU TYR
SEQRES 30 A 898 ILE ALA ARG ALA GLY TRP THR PRO GLU GLY LYS TYR ALA
SEQRES 31 A 898 TRP SER ILE LEU LEU ASP ARG SER GLN THR ARG LEU GLN
SEQRES 32 A 898 ILE VAL LEU ILE SER PRO GLU LEU PHE ILE PRO VAL GLU
SEQRES 33 A 898 ASP ASP VAL MET GLU ARG GLN ARG LEU ILE GLU SER VAL
SEQRES 34 A 898 PRO ASP SER VAL THR PRO LEU ILE ILE TYR GLU GLU THR
SEQRES 35 A 898 THR ASP ILE TRP ILE ASN ILE HIS ASP ILE PHE HIS VAL
SEQRES 36 A 898 PHE PRO GLN SER HIS GLU GLU GLU ILE GLU PHE ILE PHE
SEQRES 37 A 898 ALA SER GLU CYS LYS THR GLY PHE ARG HIS LEU TYR LYS
SEQRES 38 A 898 ILE THR SER ILE LEU LYS GLU SER LYS TYR LYS ARG SER
SEQRES 39 A 898 SER GLY GLY LEU PRO ALA PRO SER ASP PHE LYS CYS PRO
SEQRES 40 A 898 ILE LYS GLU GLU ILE ALA ILE THR SER GLY GLU TRP GLU
SEQRES 41 A 898 VAL LEU GLY ARG HIS GLY SER ASN ILE GLN VAL ASP GLU
SEQRES 42 A 898 VAL ARG ARG LEU VAL TYR PHE GLU GLY THR LYS ASP SER
SEQRES 43 A 898 PRO LEU GLU HIS HIS LEU TYR VAL VAL SER TYR VAL ASN
SEQRES 44 A 898 PRO GLY GLU VAL THR ARG LEU THR ASP ARG GLY TYR SER
SEQRES 45 A 898 HIS SER CYS CYS ILE SER GLN HIS CYS ASP PHE PHE ILE
SEQRES 46 A 898 SER LYS TYR SER ASN GLN LYS ASN PRO HIS CYS VAL SER
SEQRES 47 A 898 LEU TYR LYS LEU SER SER PRO GLU ASP ASP PRO THR CYS
SEQRES 48 A 898 LYS THR LYS GLU PHE TRP ALA THR ILE LEU ASP SER ALA
SEQRES 49 A 898 GLY PRO LEU PRO ASP TYR THR PRO PRO GLU ILE PHE SER
SEQRES 50 A 898 PHE GLU SER THR THR GLY PHE THR LEU TYR GLY MET LEU
SEQRES 51 A 898 TYR LYS PRO HIS ASP LEU GLN PRO GLY LYS LYS TYR PRO
SEQRES 52 A 898 THR VAL LEU PHE ILE TYR GLY GLY PRO GLN VAL GLN LEU
SEQRES 53 A 898 VAL ASN ASN ARG PHE LYS GLY VAL LYS TYR PHE ARG LEU
SEQRES 54 A 898 ASN THR LEU ALA SER LEU GLY TYR VAL VAL VAL VAL ILE
SEQRES 55 A 898 ASP ASN ARG GLY SER CYS HIS ARG GLY LEU LYS PHE GLU
SEQRES 56 A 898 GLY ALA PHE LYS TYR LYS MET GLY GLN ILE GLU ILE ASP
SEQRES 57 A 898 ASP GLN VAL GLU GLY LEU GLN TYR LEU ALA SER ARG TYR
SEQRES 58 A 898 ASP PHE ILE ASP LEU ASP ARG VAL GLY ILE HIS GLY TRP
SEQRES 59 A 898 SER TYR GLY GLY TYR LEU SER LEU MET ALA LEU MET GLN
SEQRES 60 A 898 ARG SER ASP ILE PHE ARG VAL ALA ILE ALA GLY ALA PRO
SEQRES 61 A 898 VAL THR LEU TRP ILE PHE TYR ASP THR GLY TYR THR GLU
SEQRES 62 A 898 ARG TYR MET GLY HIS PRO ASP GLN ASN GLU GLN GLY TYR
SEQRES 63 A 898 TYR LEU GLY SER VAL ALA MET GLN ALA GLU LYS PHE PRO
SEQRES 64 A 898 SER GLU PRO ASN ARG LEU LEU LEU LEU HIS GLY PHE LEU
SEQRES 65 A 898 ASP GLU ASN VAL HIS PHE ALA HIS THR SER ILE LEU LEU
SEQRES 66 A 898 SER PHE LEU VAL ARG ALA GLY LYS PRO TYR ASP LEU GLN
SEQRES 67 A 898 ILE TYR PRO GLN GLU ARG HIS SER ILE ARG VAL PRO GLU
SEQRES 68 A 898 SER GLY GLU HIS TYR GLU LEU HIS LEU LEU HIS TYR LEU
SEQRES 69 A 898 GLN GLU ASN LEU GLY SER ARG ILE ALA ALA LEU LYS VAL
SEQRES 70 A 898 ILE
HET TMO B 901 5
HET 2YI B 902 36
HET TMO A 901 5
HET 2YI A 902 36
HETNAM TMO TRIMETHYLAMINE OXIDE
HETNAM 2YI METHYL 3-[4-[(4-BROMOPHENYL)METHYL]PIPERAZIN-1-
HETNAM 2 2YI YL]CARBONYL-5-[(2-ETHYL-2-METHANOYL-BUTANOYL)
HETNAM 3 2YI AMINO]BENZOATE
FORMUL 3 TMO 2(C3 H9 N O)
FORMUL 4 2YI 2(C27 H32 BR N3 O5)
FORMUL 7 HOH *168(H2 O)
HELIX 1 AA1 SER B 57 ARG B 69 1 13
HELIX 2 AA2 THR B 270 ASP B 278 1 9
HELIX 3 AA3 PRO B 367 PHE B 372 1 6
HELIX 4 AA4 SER B 408 GLU B 410 5 3
HELIX 5 AA5 ASP B 418 VAL B 429 1 12
HELIX 6 AA6 LYS B 492 GLY B 496 5 5
HELIX 7 AA7 PHE B 687 LEU B 695 1 9
HELIX 8 AA8 ARG B 710 GLY B 716 1 7
HELIX 9 AA9 ALA B 717 LYS B 719 5 3
HELIX 10 AB1 ILE B 725 SER B 739 1 15
HELIX 11 AB2 SER B 755 ARG B 768 1 14
HELIX 12 AB3 LEU B 783 TYR B 787 5 5
HELIX 13 AB4 ASP B 788 GLY B 797 1 10
HELIX 14 AB5 HIS B 798 GLN B 801 5 4
HELIX 15 AB6 ASN B 802 SER B 810 1 9
HELIX 16 AB7 VAL B 811 PHE B 818 5 8
HELIX 17 AB8 HIS B 837 ALA B 851 1 15
HELIX 18 AB9 HIS B 865 ARG B 868 5 4
HELIX 19 AC1 VAL B 869 LEU B 888 1 20
HELIX 20 AC2 SER B 890 VAL B 897 1 8
HELIX 21 AC3 SER A 57 LYS A 70 1 14
HELIX 22 AC4 THR A 270 ASP A 278 1 9
HELIX 23 AC5 PRO A 367 PHE A 372 1 6
HELIX 24 AC6 SER A 408 GLU A 410 5 3
HELIX 25 AC7 ASP A 418 VAL A 429 1 12
HELIX 26 AC8 ALA A 500 LYS A 505 5 6
HELIX 27 AC9 TYR A 686 GLY A 696 1 11
HELIX 28 AD1 GLY A 711 GLY A 716 1 6
HELIX 29 AD2 ALA A 717 LYS A 719 5 3
HELIX 30 AD3 ILE A 725 TYR A 741 1 17
HELIX 31 AD4 SER A 755 ARG A 768 1 14
HELIX 32 AD5 LEU A 783 TYR A 787 5 5
HELIX 33 AD6 ASP A 788 GLY A 797 1 10
HELIX 34 AD7 HIS A 798 GLN A 801 5 4
HELIX 35 AD8 ASN A 802 GLY A 809 1 8
HELIX 36 AD9 SER A 810 PHE A 818 5 9
HELIX 37 AE1 PHE A 838 ALA A 851 1 14
HELIX 38 AE2 PRO A 870 LEU A 888 1 19
HELIX 39 AE3 SER A 890 VAL A 897 1 8
SHEET 1 AA1 5 GLU B 49 PRO B 50 0
SHEET 2 AA1 5 LYS B 660 PHE B 667 1 O LYS B 661 N GLU B 49
SHEET 3 AA1 5 VAL B 698 ILE B 702 1 O VAL B 698 N VAL B 665
SHEET 4 AA1 5 THR B 645 TYR B 651 -1 N MET B 649 O VAL B 701
SHEET 5 AA1 5 GLU B 634 GLU B 639 -1 N PHE B 636 O GLY B 648
SHEET 1 AA2 6 GLU B 49 PRO B 50 0
SHEET 2 AA2 6 LYS B 660 PHE B 667 1 O LYS B 661 N GLU B 49
SHEET 3 AA2 6 ILE B 744 TRP B 754 1 O ARG B 748 N THR B 664
SHEET 4 AA2 6 PHE B 772 GLY B 778 1 O ILE B 776 N ILE B 751
SHEET 5 AA2 6 LEU B 825 GLY B 830 1 O LEU B 826 N ALA B 777
SHEET 6 AA2 6 ASP B 856 TYR B 860 1 O ASP B 856 N LEU B 827
SHEET 1 AA3 4 HIS B 81 LYS B 87 0
SHEET 2 AA3 4 HIS B 95 ALA B 103 -1 O ARG B 98 N VAL B 86
SHEET 3 AA3 4 THR B 112 PRO B 119 -1 O ILE B 118 N ASP B 97
SHEET 4 AA3 4 LYS B 133 PRO B 134 -1 O LYS B 133 N TYR B 115
SHEET 1 AA4 4 ASP B 170 HIS B 172 0
SHEET 2 AA4 4 THR B 177 ALA B 182 -1 O LEU B 179 N ASP B 170
SHEET 3 AA4 4 GLY B 185 LYS B 190 -1 O TYR B 187 N PHE B 180
SHEET 4 AA4 4 ASN B 205 LEU B 206 -1 O ASN B 205 N HIS B 188
SHEET 1 AA5 4 MET B 216 LEU B 220 0
SHEET 2 AA5 4 TRP B 227 HIS B 232 -1 O ILE B 231 N MET B 216
SHEET 3 AA5 4 ASP B 235 ASN B 240 -1 O TRP B 237 N PHE B 230
SHEET 4 AA5 4 GLU B 246 ARG B 248 -1 O ARG B 247 N ILE B 238
SHEET 1 AA6 3 ARG B 264 ALA B 266 0
SHEET 2 AA6 3 LYS B 297 ASP B 307 -1 O ASN B 306 N SER B 265
SHEET 3 AA6 3 GLU B 290 THR B 291 -1 N GLU B 290 O ILE B 298
SHEET 1 AA7 5 TYR B 283 TRP B 285 0
SHEET 2 AA7 5 LYS B 297 ASP B 307 -1 O LEU B 302 N TRP B 284
SHEET 3 AA7 5 LYS B 340 ILE B 350 -1 O ILE B 350 N LYS B 297
SHEET 4 AA7 5 ILE B 356 LEU B 364 -1 O ILE B 360 N GLU B 347
SHEET 5 AA7 5 PHE B 412 PRO B 414 -1 O ILE B 413 N GLU B 363
SHEET 1 AA8 2 ILE B 313 HIS B 315 0
SHEET 2 AA8 2 SER B 328 ARG B 330 -1 O PHE B 329 N ILE B 314
SHEET 1 AA9 4 VAL B 375 TRP B 383 0
SHEET 2 AA9 4 ALA B 390 ASP B 396 -1 O ILE B 393 N ARG B 380
SHEET 3 AA9 4 ARG B 401 ILE B 407 -1 O ILE B 407 N ALA B 390
SHEET 4 AA9 4 LEU B 436 THR B 442 -1 O LEU B 436 N LEU B 406
SHEET 1 AB1 4 PHE B 453 VAL B 455 0
SHEET 2 AB1 4 GLU B 463 SER B 470 -1 O ILE B 467 N HIS B 454
SHEET 3 AB1 4 HIS B 478 ILE B 485 -1 O ILE B 482 N PHE B 466
SHEET 4 AB1 4 ILE B 508 ALA B 513 -1 O ILE B 512 N LYS B 481
SHEET 1 AB2 4 GLN B 530 ASP B 532 0
SHEET 2 AB2 4 LEU B 537 GLY B 542 -1 O LEU B 537 N ASP B 532
SHEET 3 AB2 4 HIS B 551 SER B 556 -1 O TYR B 553 N PHE B 540
SHEET 4 AB2 4 THR B 564 ARG B 565 -1 O THR B 564 N VAL B 554
SHEET 1 AB3 4 SER B 572 ILE B 577 0
SHEET 2 AB3 4 PHE B 583 SER B 589 -1 O LYS B 587 N SER B 574
SHEET 3 AB3 4 CYS B 596 SER B 603 -1 O TYR B 600 N PHE B 584
SHEET 4 AB3 4 THR B 613 LEU B 621 -1 O ALA B 618 N LEU B 599
SHEET 1 AB4 5 GLU A 49 PRO A 50 0
SHEET 2 AB4 5 LYS A 660 PHE A 667 1 O LYS A 661 N GLU A 49
SHEET 3 AB4 5 VAL A 698 ILE A 702 1 O VAL A 700 N PHE A 667
SHEET 4 AB4 5 THR A 645 TYR A 651 -1 N TYR A 651 O VAL A 699
SHEET 5 AB4 5 GLU A 634 GLU A 639 -1 N GLU A 634 O LEU A 650
SHEET 1 AB5 6 GLU A 49 PRO A 50 0
SHEET 2 AB5 6 LYS A 660 PHE A 667 1 O LYS A 661 N GLU A 49
SHEET 3 AB5 6 ILE A 744 TRP A 754 1 O GLY A 750 N LEU A 666
SHEET 4 AB5 6 PHE A 772 GLY A 778 1 O ILE A 776 N ILE A 751
SHEET 5 AB5 6 LEU A 825 HIS A 829 1 O LEU A 826 N ALA A 777
SHEET 6 AB5 6 ASP A 856 ILE A 859 1 O ASP A 856 N LEU A 827
SHEET 1 AB6 4 HIS A 81 LYS A 87 0
SHEET 2 AB6 4 HIS A 95 ALA A 103 -1 O ARG A 98 N VAL A 86
SHEET 3 AB6 4 THR A 112 PRO A 119 -1 O PHE A 114 N TYR A 101
SHEET 4 AB6 4 LYS A 133 PRO A 134 -1 O LYS A 133 N TYR A 115
SHEET 1 AB7 4 ASP A 170 HIS A 172 0
SHEET 2 AB7 4 THR A 177 ALA A 182 -1 O LEU A 179 N ASP A 170
SHEET 3 AB7 4 GLY A 185 LYS A 190 -1 O TYR A 187 N PHE A 180
SHEET 4 AB7 4 ASN A 205 LEU A 206 -1 O ASN A 205 N HIS A 188
SHEET 1 AB8 4 MET A 216 LEU A 220 0
SHEET 2 AB8 4 TRP A 227 HIS A 232 -1 O ILE A 231 N MET A 216
SHEET 3 AB8 4 ASP A 235 ASN A 240 -1 O SER A 239 N ILE A 228
SHEET 4 AB8 4 GLU A 246 ARG A 248 -1 O ARG A 247 N ILE A 238
SHEET 1 AB9 8 GLU A 290 THR A 292 0
SHEET 2 AB9 8 PHE A 412 PRO A 414 0
SHEET 3 AB9 8 ARG A 264 VAL A 268 0
SHEET 4 AB9 8 SER A 281 TRP A 285 -1 O GLY A 282 N GLY A 267
SHEET 5 AB9 8 GLY A 296 ASP A 307 -1 O LEU A 302 N TRP A 284
SHEET 6 AB9 8 LYS A 340 ILE A 350 -1 O LYS A 340 N ASP A 307
SHEET 7 AB9 8 ILE A 356 LEU A 364 -1 O ILE A 357 N MET A 349
SHEET 8 AB9 8 PHE A 412 PRO A 414 -1 O ILE A 413 N GLU A 363
SHEET 1 AC1 2 ILE A 313 HIS A 315 0
SHEET 2 AC1 2 SER A 328 ARG A 330 -1 O PHE A 329 N ILE A 314
SHEET 1 AC2 4 VAL A 375 TRP A 383 0
SHEET 2 AC2 4 ALA A 390 ASP A 396 -1 O TRP A 391 N GLY A 382
SHEET 3 AC2 4 ARG A 401 ILE A 407 -1 O ILE A 407 N ALA A 390
SHEET 4 AC2 4 LEU A 436 THR A 442 -1 O LEU A 436 N LEU A 406
SHEET 1 AC3 4 PHE A 453 VAL A 455 0
SHEET 2 AC3 4 GLU A 463 SER A 470 -1 O ILE A 467 N HIS A 454
SHEET 3 AC3 4 HIS A 478 ILE A 485 -1 O TYR A 480 N PHE A 468
SHEET 4 AC3 4 ILE A 508 ALA A 513 -1 O ILE A 512 N LYS A 481
SHEET 1 AC4 4 GLN A 530 ASP A 532 0
SHEET 2 AC4 4 LEU A 537 SER A 546 -1 O TYR A 539 N GLN A 530
SHEET 3 AC4 4 GLU A 549 SER A 556 -1 O TYR A 553 N PHE A 540
SHEET 4 AC4 4 THR A 564 ARG A 565 -1 O THR A 564 N VAL A 554
SHEET 1 AC5 4 SER A 572 ILE A 577 0
SHEET 2 AC5 4 PHE A 583 ASN A 590 -1 O LYS A 587 N SER A 574
SHEET 3 AC5 4 ASN A 593 SER A 603 -1 O TYR A 600 N PHE A 584
SHEET 4 AC5 4 THR A 613 LEU A 621 -1 O ALA A 618 N LEU A 599
LINK OG SER B 755 C3 2YI B 902 1555 1555 1.43
LINK OG SER A 755 C3 2YI A 902 1555 1555 1.43
CRYST1 149.443 149.443 269.388 90.00 90.00 120.00 P 61 12
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 0.006692 0.003863 0.000000 0.00000
SCALE2 0.000000 0.007727 0.000000 0.00000
SCALE3 0.000000 0.000000 0.003712 0.00000
TER 6623 VAL B 897
TER 13160 VAL A 897
MASTER 503 0 4 39 98 0 0 613401 2 84 140
END |