longtext: 7rb5-pdb

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HEADER    HYDROLASE                               05-JUL-21   7RB5
TITLE     ROOM TEMPERATURE STRUCTURE OF HACHE IN COMPLEX WITH SUBSTRATE ANALOG
TITLE    2 4K-TMA
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ACETYLCHOLINESTERASE;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: ACHE;
COMPND   5 EC: 3.1.1.7;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE   3 ORGANISM_COMMON: HUMAN;
SOURCE   4 ORGANISM_TAXID: 9606;
SOURCE   5 GENE: ACHE;
SOURCE   6 EXPRESSION_SYSTEM: HOMO SAPIENS;
SOURCE   7 EXPRESSION_SYSTEM_COMMON: HUMAN;
SOURCE   8 EXPRESSION_SYSTEM_TAXID: 9606;
SOURCE   9 EXPRESSION_SYSTEM_CELL_LINE: HEK293
KEYWDS    ACETYLCHOLINE ESTERASE, SERINE HYDROLASE, TETRAHEDRAL INTERMEDIATE,
KEYWDS   2 HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    A.KOVALEVSKY,O.GERLITS,Z.RADIC
REVDAT   1   22-SEP-21 7RB5    0
JRNL        AUTH   O.GERLITS,M.P.BLAKELEY,D.A.KEEN,Z.RADIC,A.KOVALEVSKY
JRNL        TITL   ROOM TEMPERATURE CRYSTALLOGRAPHY OF HUMAN
JRNL        TITL 2 ACETYLCHOLINESTERASE BOUND TO A SUBSTRATE ANALOGUE 4K-TMA:
JRNL        TITL 3 TOWARDS A NEUTRON STRUCTURE
JRNL        REF    CURR RES STRUCT BIOL          V.   3   206 2021
JRNL        REFN                   ESSN 2665-928X
JRNL        DOI    10.1016/J.CRSTBI.2021.08.003
REMARK   2
REMARK   2 RESOLUTION.    2.80 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (1.17.1_3660: ???)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.80
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 30.16
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.7
REMARK   3   NUMBER OF REFLECTIONS             : 28581
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.197
REMARK   3   R VALUE            (WORKING SET) : 0.195
REMARK   3   FREE R VALUE                     : 0.233
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.020
REMARK   3   FREE R VALUE TEST SET COUNT      : 1435
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 30.1600 -  6.0200    0.96     2775   115  0.1746 0.2104
REMARK   3     2  6.0200 -  4.7800    0.99     2777   142  0.1777 0.1967
REMARK   3     3  4.7800 -  4.1800    0.99     2769   129  0.1657 0.2140
REMARK   3     4  4.1800 -  3.8000    0.98     2672   170  0.1726 0.2045
REMARK   3     5  3.8000 -  3.5300    0.97     2691   135  0.1934 0.2211
REMARK   3     6  3.5300 -  3.3200    0.98     2716   155  0.2151 0.2562
REMARK   3     7  3.3200 -  3.1500    0.98     2712   130  0.2360 0.2532
REMARK   3     8  3.1500 -  3.0200    0.98     2714   132  0.2548 0.3184
REMARK   3     9  3.0200 -  2.9000    0.98     2663   160  0.2774 0.3065
REMARK   3    10  2.9000 -  2.8000    0.97     2657   167  0.2916 0.3470
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.380
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 23.330
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.005           4327
REMARK   3   ANGLE     :  0.685           5918
REMARK   3   CHIRALITY :  0.045            628
REMARK   3   PLANARITY :  0.006            784
REMARK   3   DIHEDRAL  : 13.723            600
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 7RB5 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 06-JUL-21.
REMARK 100 THE DEPOSITION ID IS D_1000257975.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 05-DEC-19
REMARK 200  TEMPERATURE           (KELVIN) : 293
REMARK 200  PH                             : 7.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : N
REMARK 200  RADIATION SOURCE               : ROTATING ANODE
REMARK 200  BEAMLINE                       : NULL
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-007 HF
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.54
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER R 4M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : CRYSALISPRO
REMARK 200  DATA SCALING SOFTWARE          : CRYSALISPRO
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 28591
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.800
REMARK 200  RESOLUTION RANGE LOW       (A) : 40.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.5
REMARK 200  DATA REDUNDANCY                : 3.500
REMARK 200  R MERGE                    (I) : 0.10000
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 12.6000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.80
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.90
REMARK 200  COMPLETENESS FOR SHELL     (%) : 97.2
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.60
REMARK 200  R MERGE FOR SHELL          (I) : 0.56600
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 1.700
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: PDB ENTRY 6O5R
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 75.14
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 4.95
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 100 MM HEPES, PH 7.5, 10 MM SODIUM
REMARK 280  CITRATE, 6-8% PEG6000, VAPOR DIFFUSION, SITTING DROP,
REMARK 280  TEMPERATURE 283K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 31 1 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -Y,X-Y,Z+1/3
REMARK 290       3555   -X+Y,-X,Z+2/3
REMARK 290       4555   -Y,-X,-Z+2/3
REMARK 290       5555   -X+Y,Y,-Z+1/3
REMARK 290       6555   X,X-Y,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       43.67467
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       87.34933
REMARK 290   SMTRY1   4  0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   4 -0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000       87.34933
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       43.67467
REMARK 290   SMTRY1   6  0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   6  0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 2870 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 39270 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: 3.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350   BIOMT1   2  0.500000  0.866025  0.000000        0.00000
REMARK 350   BIOMT2   2  0.866025 -0.500000  0.000000        0.00000
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH A 751  LIES ON A SPECIAL POSITION.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     GLY A    -2
REMARK 465     PRO A    -1
REMARK 465     LEU A     0
REMARK 465     GLU A     1
REMARK 465     GLY A     2
REMARK 465     ARG A     3
REMARK 465     ASP A   544
REMARK 465     THR A   545
REMARK 465     LEU A   546
REMARK 465     ASP A   547
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PHE A  47       -0.57     71.62
REMARK 500    CYS A  96       -5.16   -149.95
REMARK 500    PHE A 123       13.83     59.59
REMARK 500    ASN A 186      -13.94   -148.60
REMARK 500    PRO A 194        2.98    -68.73
REMARK 500    SER A 203     -119.46     52.40
REMARK 500    ASP A 306      -77.70   -106.27
REMARK 500    VAL A 407      -62.58   -122.96
REMARK 500    GLN A 421       26.52   -141.36
REMARK 500    ASN A 464       53.57    -90.58
REMARK 500    ASP A 514     -167.64   -160.90
REMARK 500
REMARK 500 REMARK: NULL
DBREF  7RB5 A    1   547  UNP    P22303   ACES_HUMAN      32    578
SEQADV 7RB5 GLY A   -2  UNP  P22303              EXPRESSION TAG
SEQADV 7RB5 PRO A   -1  UNP  P22303              EXPRESSION TAG
SEQADV 7RB5 LEU A    0  UNP  P22303              EXPRESSION TAG
SEQRES   1 A  550  GLY PRO LEU GLU GLY ARG GLU ASP ALA GLU LEU LEU VAL
SEQRES   2 A  550  THR VAL ARG GLY GLY ARG LEU ARG GLY ILE ARG LEU LYS
SEQRES   3 A  550  THR PRO GLY GLY PRO VAL SER ALA PHE LEU GLY ILE PRO
SEQRES   4 A  550  PHE ALA GLU PRO PRO MET GLY PRO ARG ARG PHE LEU PRO
SEQRES   5 A  550  PRO GLU PRO LYS GLN PRO TRP SER GLY VAL VAL ASP ALA
SEQRES   6 A  550  THR THR PHE GLN SER VAL CYS TYR GLN TYR VAL ASP THR
SEQRES   7 A  550  LEU TYR PRO GLY PHE GLU GLY THR GLU MET TRP ASN PRO
SEQRES   8 A  550  ASN ARG GLU LEU SER GLU ASP CYS LEU TYR LEU ASN VAL
SEQRES   9 A  550  TRP THR PRO TYR PRO ARG PRO THR SER PRO THR PRO VAL
SEQRES  10 A  550  LEU VAL TRP ILE TYR GLY GLY GLY PHE TYR SER GLY ALA
SEQRES  11 A  550  SER SER LEU ASP VAL TYR ASP GLY ARG PHE LEU VAL GLN
SEQRES  12 A  550  ALA GLU ARG THR VAL LEU VAL SER MET ASN TYR ARG VAL
SEQRES  13 A  550  GLY ALA PHE GLY PHE LEU ALA LEU PRO GLY SER ARG GLU
SEQRES  14 A  550  ALA PRO GLY ASN VAL GLY LEU LEU ASP GLN ARG LEU ALA
SEQRES  15 A  550  LEU GLN TRP VAL GLN GLU ASN VAL ALA ALA PHE GLY GLY
SEQRES  16 A  550  ASP PRO THR SER VAL THR LEU PHE GLY GLU SER ALA GLY
SEQRES  17 A  550  ALA ALA SER VAL GLY MET HIS LEU LEU SER PRO PRO SER
SEQRES  18 A  550  ARG GLY LEU PHE HIS ARG ALA VAL LEU GLN SER GLY ALA
SEQRES  19 A  550  PRO ASN GLY PRO TRP ALA THR VAL GLY MET GLY GLU ALA
SEQRES  20 A  550  ARG ARG ARG ALA THR GLN LEU ALA HIS LEU VAL GLY CYS
SEQRES  21 A  550  PRO PRO GLY GLY THR GLY GLY ASN ASP THR GLU LEU VAL
SEQRES  22 A  550  ALA CYS LEU ARG THR ARG PRO ALA GLN VAL LEU VAL ASN
SEQRES  23 A  550  HIS GLU TRP HIS VAL LEU PRO GLN GLU SER VAL PHE ARG
SEQRES  24 A  550  PHE SER PHE VAL PRO VAL VAL ASP GLY ASP PHE LEU SER
SEQRES  25 A  550  ASP THR PRO GLU ALA LEU ILE ASN ALA GLY ASP PHE HIS
SEQRES  26 A  550  GLY LEU GLN VAL LEU VAL GLY VAL VAL LYS ASP GLU GLY
SEQRES  27 A  550  SER TYR PHE LEU VAL TYR GLY ALA PRO GLY PHE SER LYS
SEQRES  28 A  550  ASP ASN GLU SER LEU ILE SER ARG ALA GLU PHE LEU ALA
SEQRES  29 A  550  GLY VAL ARG VAL GLY VAL PRO GLN VAL SER ASP LEU ALA
SEQRES  30 A  550  ALA GLU ALA VAL VAL LEU HIS TYR THR ASP TRP LEU HIS
SEQRES  31 A  550  PRO GLU ASP PRO ALA ARG LEU ARG GLU ALA LEU SER ASP
SEQRES  32 A  550  VAL VAL GLY ASP HIS ASN VAL VAL CYS PRO VAL ALA GLN
SEQRES  33 A  550  LEU ALA GLY ARG LEU ALA ALA GLN GLY ALA ARG VAL TYR
SEQRES  34 A  550  ALA TYR VAL PHE GLU HIS ARG ALA SER THR LEU SER TRP
SEQRES  35 A  550  PRO LEU TRP MET GLY VAL PRO HIS GLY TYR GLU ILE GLU
SEQRES  36 A  550  PHE ILE PHE GLY ILE PRO LEU ASP PRO SER ARG ASN TYR
SEQRES  37 A  550  THR ALA GLU GLU LYS ILE PHE ALA GLN ARG LEU MET ARG
SEQRES  38 A  550  TYR TRP ALA ASN PHE ALA ARG THR GLY ASP PRO ASN GLU
SEQRES  39 A  550  PRO ARG ASP PRO LYS ALA PRO GLN TRP PRO PRO TYR THR
SEQRES  40 A  550  ALA GLY ALA GLN GLN TYR VAL SER LEU ASP LEU ARG PRO
SEQRES  41 A  550  LEU GLU VAL ARG ARG GLY LEU ARG ALA GLN ALA CYS ALA
SEQRES  42 A  550  PHE TRP ASN ARG PHE LEU PRO LYS LEU LEU SER ALA THR
SEQRES  43 A  550  ASP THR LEU ASP
HET    NWA  A 601      10
HETNAM     NWA 4,4-DIHYDROXY-N,N,N-TRIMETHYLPENTAN-1-AMINIUM
FORMUL   2  NWA    C8 H20 N O2 1+
FORMUL   3  HOH   *56(H2 O)
HELIX    1 AA1 GLU A    4  GLU A    7  5                                   4
HELIX    2 AA2 MET A   42  ARG A   46  5                                   5
HELIX    3 AA3 PHE A   80  MET A   85  1                                   6
HELIX    4 AA4 LEU A  130  ASP A  134  5                                   5
HELIX    5 AA5 GLY A  135  ARG A  143  1                                   9
HELIX    6 AA6 VAL A  153  LEU A  159  1                                   7
HELIX    7 AA7 ASN A  170  VAL A  187  1                                  18
HELIX    8 AA8 ALA A  188  PHE A  190  5                                   3
HELIX    9 AA9 SER A  203  LEU A  214  1                                  12
HELIX   10 AB1 SER A  215  GLY A  220  1                                   6
HELIX   11 AB2 GLY A  240  VAL A  255  1                                  16
HELIX   12 AB3 ASN A  265  ARG A  274  1                                  10
HELIX   13 AB4 PRO A  277  HIS A  284  1                                   8
HELIX   14 AB5 GLU A  285  LEU A  289  5                                   5
HELIX   15 AB6 THR A  311  ALA A  318  1                                   8
HELIX   16 AB7 GLY A  335  VAL A  340  1                                   6
HELIX   17 AB8 SER A  355  VAL A  367  1                                  13
HELIX   18 AB9 SER A  371  THR A  383  1                                  13
HELIX   19 AC1 ASP A  390  VAL A  407  1                                  18
HELIX   20 AC2 VAL A  407  ALA A  420  1                                  14
HELIX   21 AC3 PRO A  440  GLY A  444  5                                   5
HELIX   22 AC4 GLU A  450  PHE A  455  1                                   6
HELIX   23 AC5 GLY A  456  ASP A  460  5                                   5
HELIX   24 AC6 THR A  466  GLY A  487  1                                  22
HELIX   25 AC7 ARG A  525  ARG A  534  1                                  10
HELIX   26 AC8 ARG A  534  ALA A  542  1                                   9
SHEET    1 AA1 3 LEU A   9  THR A  11  0
SHEET    2 AA1 3 ARG A  16  ARG A  18 -1  O  LEU A  17   N  VAL A  10
SHEET    3 AA1 3 VAL A  59  ASP A  61  1  O  VAL A  60   N  ARG A  16
SHEET    1 AA211 ILE A  20  THR A  24  0
SHEET    2 AA211 GLY A  27  PRO A  36 -1  O  ALA A  31   N  ILE A  20
SHEET    3 AA211 TYR A  98  PRO A 104 -1  O  THR A 103   N  SER A  30
SHEET    4 AA211 VAL A 145  MET A 149 -1  O  SER A 148   N  ASN A 100
SHEET    5 AA211 THR A 112  ILE A 118  1  N  TRP A 117   O  VAL A 147
SHEET    6 AA211 GLY A 192  GLU A 202  1  O  ASP A 193   N  THR A 112
SHEET    7 AA211 ARG A 224  GLN A 228  1  O  GLN A 228   N  GLY A 201
SHEET    8 AA211 GLN A 325  VAL A 331  1  O  LEU A 327   N  LEU A 227
SHEET    9 AA211 ARG A 424  PHE A 430  1  O  PHE A 430   N  VAL A 330
SHEET   10 AA211 GLN A 509  LEU A 513  1  O  LEU A 513   N  VAL A 429
SHEET   11 AA211 GLU A 519  ARG A 522 -1  O  ARG A 521   N  TYR A 510
SSBOND   1 CYS A   69    CYS A   96                          1555   1555  2.03
SSBOND   2 CYS A  257    CYS A  272                          1555   1555  2.05
SSBOND   3 CYS A  409    CYS A  529                          1555   1555  2.09
LINK         OG  SER A 203                 C5  NWA A 601     1555   1555  1.40
CISPEP   1 TYR A  105    PRO A  106          0         1.76
CISPEP   2 CYS A  257    PRO A  258          0         2.73
CISPEP   3 GLY A  260    GLY A  261          0        -0.53
CRYST1  125.583  125.583  131.024  90.00  90.00 120.00 P 31 1 2      6
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.007963  0.004597  0.000000        0.00000
SCALE2      0.000000  0.009195  0.000000        0.00000
SCALE3      0.000000  0.000000  0.007632        0.00000
TER    4189      THR A 543
MASTER      266    0    1   26   14    0    0    6 4254    1   17   43
END