longtext: 7wwh-pdb

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HEADER    HYDROLASE                               12-FEB-22   7WWH
TITLE     CRYSTAL STRUCTURE OF THE GEOBACILLUS THERMOGLUCOSIDASIUS FERULOYL
TITLE    2 ESTERASE GTHFAE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ALPHA/BETA HYDROLASE;
COMPND   3 CHAIN: A, B;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: PARAGEOBACILLUS THERMOGLUCOSIDASIUS;
SOURCE   3 ORGANISM_TAXID: 1426;
SOURCE   4 GENE: DV712_01095;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008
KEYWDS    ALPHA/BETA HYDROLASE, FERULOYL ESTERASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    W.YANG,Y.WU
REVDAT   1   09-MAR-22 7WWH    0
JRNL        AUTH   W.YANG,L.SUN,P.DONG,Y.CHEN,H.ZHANG,X.HUANG,L.WU,L.CHEN,
JRNL        AUTH 2 D.JING,Y.WU
JRNL        TITL   STRUCTURE-GUIDED RATIONAL DESIGN OF THE GEOBACILLUS
JRNL        TITL 2 THERMOGLUCOSIDASIUS FERULOYL ESTERASE GTHFAE TO IMPROVE ITS
JRNL        TITL 3 THERMOSTABILITY.
JRNL        REF    BIOCHEM.BIOPHYS.RES.COMMUN.   V. 600   117 2022
JRNL        REFN                   ESSN 1090-2104
JRNL        PMID   35219099
JRNL        DOI    10.1016/J.BBRC.2022.02.074
REMARK   2
REMARK   2 RESOLUTION.    1.92 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.18.2_3874
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.92
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 26.29
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.410
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.1
REMARK   3   NUMBER OF REFLECTIONS             : 41098
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.176
REMARK   3   R VALUE            (WORKING SET) : 0.174
REMARK   3   FREE R VALUE                     : 0.203
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.950
REMARK   3   FREE R VALUE TEST SET COUNT      : 2035
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 26.2900 -  4.7200    0.99     2717   129  0.1761 0.1916
REMARK   3     2  4.7200 -  3.7500    1.00     2675   131  0.1466 0.1580
REMARK   3     3  3.7500 -  3.2800    0.97     2568   139  0.1653 0.1664
REMARK   3     4  3.2800 -  2.9800    0.99     2674   140  0.1789 0.2273
REMARK   3     5  2.9800 -  2.7700    1.00     2628   146  0.1753 0.2264
REMARK   3     6  2.7700 -  2.6000    1.00     2624   145  0.1866 0.1997
REMARK   3     7  2.6000 -  2.4700    1.00     2639   139  0.1851 0.2262
REMARK   3     8  2.4700 -  2.3600    0.98     2639   134  0.1776 0.2421
REMARK   3     9  2.3600 -  2.2700    0.99     2622   125  0.1791 0.2158
REMARK   3    10  2.2700 -  2.2000    1.00     2601   154  0.1778 0.2112
REMARK   3    11  2.2000 -  2.1300    1.00     2653   138  0.1760 0.2267
REMARK   3    12  2.1300 -  2.0700    0.99     2623   140  0.1906 0.2593
REMARK   3    13  2.0700 -  2.0100    0.98     2595   128  0.2006 0.2504
REMARK   3    14  2.0100 -  1.9600    0.97     2580   128  0.2067 0.2539
REMARK   3    15  1.9600 -  1.9200    0.86     2225   119  0.2099 0.2557
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.180
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 21.290
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 32.64
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :   NULL           NULL
REMARK   3   ANGLE     :   NULL           NULL
REMARK   3   CHIRALITY :   NULL           NULL
REMARK   3   PLANARITY :   NULL           NULL
REMARK   3   DIHEDRAL  :   NULL           NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 7WWH COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 18-FEB-22.
REMARK 100 THE DEPOSITION ID IS D_1300027644.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 24-MAY-21
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 7.0
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRF
REMARK 200  BEAMLINE                       : BL17B1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.979
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : RAYONIX MX-300
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 41148
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.910
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.7
REMARK 200  DATA REDUNDANCY                : 6.700
REMARK 200  R MERGE                    (I) : 0.07300
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 5.6000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.91
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.94
REMARK 200  COMPLETENESS FOR SHELL     (%) : 94.6
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.20
REMARK 200  R MERGE FOR SHELL          (I) : 0.50500
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: 3PF8
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 45.38
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.51
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M TRIS-HCL PH 7.0, 20% PEG 1000,
REMARK 280  VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 289K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       43.70500
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     0
REMARK 465     MET B     0
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    GLU A  43      167.03     64.26
REMARK 500    SER A 113     -108.69     57.36
REMARK 500    ASN A 141       40.89    -94.26
REMARK 500    GLU B  43      169.29     65.51
REMARK 500    SER B 113     -110.94     51.45
REMARK 500    ALA B 139       78.83   -108.90
REMARK 500    ASN B 141       58.89    -98.79
REMARK 500    GLN B 152      -74.26   -102.61
REMARK 500    ASN B 154       76.78     57.13
REMARK 500
REMARK 500 REMARK: NULL
DBREF1 7WWH A    0   251  UNP                  A0A369WTY0_PARTM
DBREF2 7WWH A     A0A369WTY0                          1         252
DBREF1 7WWH B    0   251  UNP                  A0A369WTY0_PARTM
DBREF2 7WWH B     A0A369WTY0                          1         252
SEQRES   1 A  252  MET GLN LYS ALA ILE THR LEU THR HIS ARG GLY MET THR
SEQRES   2 A  252  LEU ARG GLY MET GLU HIS ILE PRO GLU LYS SER LEU ASP
SEQRES   3 A  252  GLU LYS VAL PRO ALA VAL ILE LEU PHE HIS GLY PHE THR
SEQRES   4 A  252  GLY THR LYS LEU GLU PRO HIS ARG LEU PHE LEU LYS ILE
SEQRES   5 A  252  SER ARG ALA LEU GLU LYS GLN GLY ILE ALA SER PHE ARG
SEQRES   6 A  252  PHE ASP PHE LEU GLY SER GLY GLU SER ASP GLY ASP PHE
SEQRES   7 A  252  GLU GLU MET THR VAL SER LYS GLU ILE GLU GLU ALA HIS
SEQRES   8 A  252  ALA ILE VAL ASP PHE VAL LYS ARG ASP GLY ARG ILE ASP
SEQRES   9 A  252  PRO SER HIS ILE TYR LEU LEU GLY LEU SER MET GLY GLY
SEQRES  10 A  252  LEU VAL ALA SER VAL VAL ALA GLY GLU ARG PRO ASN ASP
SEQRES  11 A  252  VAL ALA LYS LEU ILE LEU MET ALA PRO ALA GLY ASN MET
SEQRES  12 A  252  TYR GLU LEU ILE THR GLU THR ILE ARG GLN GLU ASN ILE
SEQRES  13 A  252  ASP VAL THR ALA PRO TYR PHE ASP HIS GLY GLY ASN LEU
SEQRES  14 A  252  VAL GLY ARG SER PHE LEU GLU ASP LEU GLN THR ILE ASN
SEQRES  15 A  252  VAL PHE GLU ARG ALA LYS PRO TYR ASP GLY PRO VAL LEU
SEQRES  16 A  252  LEU ILE HIS GLY THR GLU ASP ASP VAL VAL PRO HIS ARG
SEQRES  17 A  252  VAL SER HIS LEU TYR GLU GLN LEU CYS TYR GLY SER ARG
SEQRES  18 A  252  ALA THR VAL HIS LEU ILE GLU GLY ALA ASN HIS THR PHE
SEQRES  19 A  252  ASP GLY HIS ARG TRP GLU THR GLU VAL ILE LYS THR ILE
SEQRES  20 A  252  LEU GLY PHE VAL SER
SEQRES   1 B  252  MET GLN LYS ALA ILE THR LEU THR HIS ARG GLY MET THR
SEQRES   2 B  252  LEU ARG GLY MET GLU HIS ILE PRO GLU LYS SER LEU ASP
SEQRES   3 B  252  GLU LYS VAL PRO ALA VAL ILE LEU PHE HIS GLY PHE THR
SEQRES   4 B  252  GLY THR LYS LEU GLU PRO HIS ARG LEU PHE LEU LYS ILE
SEQRES   5 B  252  SER ARG ALA LEU GLU LYS GLN GLY ILE ALA SER PHE ARG
SEQRES   6 B  252  PHE ASP PHE LEU GLY SER GLY GLU SER ASP GLY ASP PHE
SEQRES   7 B  252  GLU GLU MET THR VAL SER LYS GLU ILE GLU GLU ALA HIS
SEQRES   8 B  252  ALA ILE VAL ASP PHE VAL LYS ARG ASP GLY ARG ILE ASP
SEQRES   9 B  252  PRO SER HIS ILE TYR LEU LEU GLY LEU SER MET GLY GLY
SEQRES  10 B  252  LEU VAL ALA SER VAL VAL ALA GLY GLU ARG PRO ASN ASP
SEQRES  11 B  252  VAL ALA LYS LEU ILE LEU MET ALA PRO ALA GLY ASN MET
SEQRES  12 B  252  TYR GLU LEU ILE THR GLU THR ILE ARG GLN GLU ASN ILE
SEQRES  13 B  252  ASP VAL THR ALA PRO TYR PHE ASP HIS GLY GLY ASN LEU
SEQRES  14 B  252  VAL GLY ARG SER PHE LEU GLU ASP LEU GLN THR ILE ASN
SEQRES  15 B  252  VAL PHE GLU ARG ALA LYS PRO TYR ASP GLY PRO VAL LEU
SEQRES  16 B  252  LEU ILE HIS GLY THR GLU ASP ASP VAL VAL PRO HIS ARG
SEQRES  17 B  252  VAL SER HIS LEU TYR GLU GLN LEU CYS TYR GLY SER ARG
SEQRES  18 B  252  ALA THR VAL HIS LEU ILE GLU GLY ALA ASN HIS THR PHE
SEQRES  19 B  252  ASP GLY HIS ARG TRP GLU THR GLU VAL ILE LYS THR ILE
SEQRES  20 B  252  LEU GLY PHE VAL SER
FORMUL   3  HOH   *417(H2 O)
HELIX    1 AA1 PRO A   20  ASP A   25  5                                   6
HELIX    2 AA2 GLU A   43  HIS A   45  5                                   3
HELIX    3 AA3 ARG A   46  GLN A   58  1                                  13
HELIX    4 AA4 ASP A   76  MET A   80  5                                   5
HELIX    5 AA5 THR A   81  ARG A   98  1                                  18
HELIX    6 AA6 SER A  113  ARG A  126  1                                  14
HELIX    7 AA7 PRO A  127  VAL A  130  5                                   4
HELIX    8 AA8 ASN A  141  GLU A  153  1                                  13
HELIX    9 AA9 ARG A  171  THR A  179  1                                   9
HELIX   10 AB1 ASN A  181  LYS A  187  1                                   7
HELIX   11 AB2 HIS A  206  CYS A  216  1                                  11
HELIX   12 AB3 TYR A  217  SER A  219  5                                   3
HELIX   13 AB4 GLY A  235  SER A  251  1                                  17
HELIX   14 AB5 PRO B   20  ASP B   25  5                                   6
HELIX   15 AB6 GLU B   43  HIS B   45  5                                   3
HELIX   16 AB7 ARG B   46  GLN B   58  1                                  13
HELIX   17 AB8 ASP B   76  MET B   80  5                                   5
HELIX   18 AB9 THR B   81  ARG B   98  1                                  18
HELIX   19 AC1 SER B  113  ARG B  126  1                                  14
HELIX   20 AC2 PRO B  127  VAL B  130  5                                   4
HELIX   21 AC3 TYR B  143  GLU B  148  1                                   6
HELIX   22 AC4 ARG B  171  THR B  179  1                                   9
HELIX   23 AC5 ASN B  181  LYS B  187  1                                   7
HELIX   24 AC6 ARG B  207  CYS B  216  1                                  10
HELIX   25 AC7 TYR B  217  SER B  219  5                                   3
HELIX   26 AC8 GLY B  235  SER B  251  1                                  17
SHEET    1 AA1 8 LYS A   2  HIS A   8  0
SHEET    2 AA1 8 MET A  11  HIS A  18 -1  O  LEU A  13   N  LEU A   6
SHEET    3 AA1 8 ALA A  61  PHE A  65 -1  O  SER A  62   N  HIS A  18
SHEET    4 AA1 8 VAL A  28  PHE A  34  1  N  PRO A  29   O  ALA A  61
SHEET    5 AA1 8 ILE A 102  LEU A 112  1  O  TYR A 108   N  ALA A  30
SHEET    6 AA1 8 LYS A 132  MET A 136  1  O  ILE A 134   N  LEU A 109
SHEET    7 AA1 8 VAL A 193  GLY A 198  1  O  LEU A 194   N  LEU A 135
SHEET    8 AA1 8 ALA A 221  ILE A 226  1  O  HIS A 224   N  LEU A 195
SHEET    1 AA2 2 TYR A 161  HIS A 164  0
SHEET    2 AA2 2 ASN A 167  GLY A 170 -1  O  VAL A 169   N  PHE A 162
SHEET    1 AA3 8 LYS B   2  HIS B   8  0
SHEET    2 AA3 8 MET B  11  HIS B  18 -1  O  MET B  11   N  HIS B   8
SHEET    3 AA3 8 ALA B  61  PHE B  65 -1  O  SER B  62   N  HIS B  18
SHEET    4 AA3 8 VAL B  28  PHE B  34  1  N  PRO B  29   O  ALA B  61
SHEET    5 AA3 8 ILE B 102  LEU B 112  1  O  TYR B 108   N  ALA B  30
SHEET    6 AA3 8 LYS B 132  MET B 136  1  O  MET B 136   N  GLY B 111
SHEET    7 AA3 8 VAL B 193  GLY B 198  1  O  LEU B 194   N  LEU B 135
SHEET    8 AA3 8 ALA B 221  ILE B 226  1  O  HIS B 224   N  LEU B 195
SHEET    1 AA4 2 TYR B 161  HIS B 164  0
SHEET    2 AA4 2 ASN B 167  GLY B 170 -1  O  VAL B 169   N  PHE B 162
CRYST1   46.038   87.410   69.456  90.00  95.97  90.00 P 1 21 1      4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.021721  0.000000  0.002270        0.00000
SCALE2      0.000000  0.011440  0.000000        0.00000
SCALE3      0.000000  0.000000  0.014476        0.00000
TER    1975      SER A 251
TER    3950      SER B 251
MASTER      235    0    0   26   20    0    0    6 4365    2    0   40
END