longtext: 8hfw-pdb

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HEADER    HYDROLASE                               12-NOV-22   8HFW
TITLE     THE CRYSTAL STRUCTURE OF ALPHA/BETA FOLD HYDROLASE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ALPHA/BETA FOLD HYDROLASE;
COMPND   3 CHAIN: A, B, C;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: BURKHOLDERIA PYRROCINIA;
SOURCE   3 ORGANISM_COMMON: PSEUDOMONAS PYRROCINIA;
SOURCE   4 ORGANISM_TAXID: 60550;
SOURCE   5 GENE: EVG18_06755;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008
KEYWDS    ESTER SYNTHETASE, CARBOXYLESTERASE, SMALL MOLECULE FATTY ACID
KEYWDS   2 ESTERASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    H.LU,Y.XU
REVDAT   1   24-MAY-23 8HFW    0
JRNL        AUTH   H.LU,Y.XU
JRNL        TITL   THE CRYSTAL STRUCTURE OF ALPHA/BETA FOLD HYDROLASE
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.66 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.8.0352
REMARK   3   AUTHORS     : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK   3               : NICHOLLS,WINN,LONG,VAGIN
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.66
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 57.64
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.4
REMARK   3   NUMBER OF REFLECTIONS             : 98506
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : FREE R-VALUE
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL
REMARK   3   R VALUE     (WORKING + TEST SET) : NULL
REMARK   3   R VALUE            (WORKING SET) : 0.194
REMARK   3   FREE R VALUE                     : 0.229
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.992
REMARK   3   FREE R VALUE TEST SET COUNT      : 4917
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.66
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.70
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 6724
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 95.25
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2080
REMARK   3   BIN FREE R VALUE SET COUNT          : 350
REMARK   3   BIN FREE R VALUE                    : 0.2880
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 5977
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 0
REMARK   3   SOLVENT ATOMS            : 329
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 27.20
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.22700
REMARK   3    B22 (A**2) : -0.98300
REMARK   3    B33 (A**2) : 0.67900
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.57800
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.136
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.101
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.078
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 5.215
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.957
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.951
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  6144 ; 0.008 ; 0.012
REMARK   3   BOND LENGTHS OTHERS               (A):  5350 ; 0.001 ; 0.016
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  8380 ; 1.478 ; 1.643
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 12361 ; 0.449 ; 1.564
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   846 ; 5.379 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):    31 ; 3.461 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   701 ;14.840 ;10.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   920 ; 0.065 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  7280 ; 0.007 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):  1226 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  1407 ; 0.229 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):    39 ; 0.129 ; 0.200
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  3084 ; 0.179 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   227 ; 0.109 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  3375 ; 2.732 ; 1.467
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  3375 ; 2.732 ; 1.467
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  4206 ; 3.322 ; 2.203
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  4207 ; 3.322 ; 2.203
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  2769 ; 3.268 ; 1.624
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  2770 ; 3.268 ; 1.624
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  4169 ; 3.753 ; 2.363
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  4170 ; 3.753 ; 2.363
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2): 11494 ; 5.374 ; 3.000
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 3
REMARK   3
REMARK   3   TLS GROUP : 1
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A    13        A   297
REMARK   3    ORIGIN FOR THE GROUP (A):  32.2730  -0.3538  33.0472
REMARK   3    T TENSOR
REMARK   3      T11:   0.0037 T22:   0.0445
REMARK   3      T33:   0.0052 T12:   0.0007
REMARK   3      T13:  -0.0004 T23:   0.0030
REMARK   3    L TENSOR
REMARK   3      L11:   1.1097 L22:   1.4567
REMARK   3      L33:   1.0953 L12:  -0.1455
REMARK   3      L13:   0.4669 L23:   0.1449
REMARK   3    S TENSOR
REMARK   3      S11:   0.0167 S12:  -0.0145 S13:  -0.0685
REMARK   3      S21:   0.0489 S22:   0.0304 S23:   0.0246
REMARK   3      S31:  -0.0106 S32:  -0.0108 S33:  -0.0471
REMARK   3
REMARK   3   TLS GROUP : 2
REMARK   3    NUMBER OF COMPONENTS GROUP : 0
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    ORIGIN FOR THE GROUP (A):  26.9184   2.1220   3.0456
REMARK   3    T TENSOR
REMARK   3      T11:   0.2118 T22:   0.1507
REMARK   3      T33:   0.0208 T12:   0.0380
REMARK   3      T13:  -0.0493 T23:  -0.0214
REMARK   3    L TENSOR
REMARK   3      L11:   1.5548 L22:   1.3685
REMARK   3      L33:   2.7571 L12:  -0.3278
REMARK   3      L13:  -0.9805 L23:   0.2873
REMARK   3    S TENSOR
REMARK   3      S11:   0.1508 S12:   0.2540 S13:  -0.0790
REMARK   3      S21:  -0.3838 S22:  -0.1009 S23:   0.0717
REMARK   3      S31:  -0.1958 S32:  -0.0201 S33:  -0.0499
REMARK   3
REMARK   3   TLS GROUP : 3
REMARK   3    NUMBER OF COMPONENTS GROUP : 0
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    ORIGIN FOR THE GROUP (A):  -6.2193  25.7536  23.1714
REMARK   3    T TENSOR
REMARK   3      T11:   0.1858 T22:   0.1817
REMARK   3      T33:   0.1855 T12:   0.0605
REMARK   3      T13:  -0.0185 T23:   0.0629
REMARK   3    L TENSOR
REMARK   3      L11:   1.6398 L22:   1.1887
REMARK   3      L33:   1.8353 L12:   0.1201
REMARK   3      L13:  -0.3320 L23:  -0.0070
REMARK   3    S TENSOR
REMARK   3      S11:   0.0325 S12:   0.3239 S13:   0.3227
REMARK   3      S21:  -0.2133 S22:  -0.0315 S23:   0.0505
REMARK   3      S31:  -0.4520 S32:  -0.1330 S33:  -0.0010
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK BULK SOLVENT
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THEIR
REMARK   3  RIDING POSITIONS
REMARK   4
REMARK   4 8HFW COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBC ON 15-NOV-22.
REMARK 100 THE DEPOSITION ID IS D_1300033400.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 18-OCT-21
REMARK 200  TEMPERATURE           (KELVIN) : 298
REMARK 200  PH                             : 7.0-7.2
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRF
REMARK 200  BEAMLINE                       : BL02U1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.979
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER2 S 9M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XSCALE
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 98523
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.660
REMARK 200  RESOLUTION RANGE LOW       (A) : 57.640
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.4
REMARK 200  DATA REDUNDANCY                : 6.370
REMARK 200  R MERGE                    (I) : 0.04800
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 19.8000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.66
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.70
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 0.46600
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: BALBES
REMARK 200 STARTING MODEL: 5Y57
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 48.49
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.39
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M BIS-TRIS, PH7.2, 0.2 M MAGNESIUM
REMARK 280  ACETATE, 17% PEG3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE
REMARK 280  289K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y,-Z
REMARK 290       3555   X+1/2,Y+1/2,Z
REMARK 290       4555   -X+1/2,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       83.48000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       34.20500
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       83.48000
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       34.20500
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 2470 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 19340 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -17.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 2370 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 19830 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -16.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000       -4.69783
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000       75.19339
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     GLU A     2
REMARK 465     THR A     3
REMARK 465     ASN A     4
REMARK 465     VAL A     5
REMARK 465     THR A     6
REMARK 465     ALA A     7
REMARK 465     ALA A     8
REMARK 465     ALA A     9
REMARK 465     PRO A    10
REMARK 465     SER A    11
REMARK 465     ASP A    12
REMARK 465     GLU A   298
REMARK 465     HIS A   299
REMARK 465     HIS A   300
REMARK 465     HIS A   301
REMARK 465     HIS A   302
REMARK 465     HIS A   303
REMARK 465     HIS A   304
REMARK 465     MET B     1
REMARK 465     GLU B     2
REMARK 465     THR B     3
REMARK 465     ASN B     4
REMARK 465     VAL B     5
REMARK 465     THR B     6
REMARK 465     ALA B     7
REMARK 465     ALA B     8
REMARK 465     ALA B     9
REMARK 465     PRO B    10
REMARK 465     SER B    11
REMARK 465     ASP B    12
REMARK 465     ARG B   150
REMARK 465     PRO B   167
REMARK 465     ILE B   295
REMARK 465     ALA B   296
REMARK 465     LEU B   297
REMARK 465     GLU B   298
REMARK 465     HIS B   299
REMARK 465     HIS B   300
REMARK 465     HIS B   301
REMARK 465     HIS B   302
REMARK 465     HIS B   303
REMARK 465     HIS B   304
REMARK 465     MET C     1
REMARK 465     GLU C     2
REMARK 465     THR C     3
REMARK 465     ASN C     4
REMARK 465     VAL C     5
REMARK 465     THR C     6
REMARK 465     ALA C     7
REMARK 465     ALA C     8
REMARK 465     ALA C     9
REMARK 465     PRO C    10
REMARK 465     SER C    11
REMARK 465     ASP C    12
REMARK 465     ALA C   226
REMARK 465     ILE C   295
REMARK 465     ALA C   296
REMARK 465     LEU C   297
REMARK 465     GLU C   298
REMARK 465     HIS C   299
REMARK 465     HIS C   300
REMARK 465     HIS C   301
REMARK 465     HIS C   302
REMARK 465     HIS C   303
REMARK 465     HIS C   304
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     GLU A  38    CG   CD   OE1  OE2
REMARK 470     LEU A  42    CD1
REMARK 470     ILE A  53    CD1
REMARK 470     LEU A  62    CD2
REMARK 470     GLU A  63    CG   CD   OE1  OE2
REMARK 470     PRO A  65    CB   CG
REMARK 470     LEU A  66    CD1  CD2
REMARK 470     LYS A  68    CG   CD   CE   NZ
REMARK 470     ASP A  69    OD2
REMARK 470     THR A  88    CG2
REMARK 470     LEU A 100    O    CD1  CD2
REMARK 470     HIS A 102    CG   ND1  CD2  CE1  NE2
REMARK 470     ILE A 131    CD1
REMARK 470     LYS A 155    CE   NZ
REMARK 470     LEU A 159    CD1  CD2
REMARK 470     ALA A 160    CB
REMARK 470     ARG A 168    CG   CD   NE   CZ   NH1  NH2
REMARK 470     MET A 204    CE
REMARK 470     VAL A 260    CG1  CG2
REMARK 470     ASP A 271    OD1  OD2
REMARK 470     LYS A 293    CE   NZ
REMARK 470     ILE A 295    CG1  CD1
REMARK 470     ALA A 296    CB
REMARK 470     LEU A 297    CA   C    O    CB   CG   CD1  CD2
REMARK 470     HIS B  13    CD2  CE1  NE2
REMARK 470     VAL B  15    CG1
REMARK 470     GLU B  38    CG   CD   OE1  OE2
REMARK 470     ARG B  39    NH1
REMARK 470     LEU B  42    CD1  CD2
REMARK 470     ILE B  53    CD1
REMARK 470     PRO B  65    CG   CD
REMARK 470     LYS B  68    CG   CD   CE   NZ
REMARK 470     ASP B  69    CG   OD1  OD2
REMARK 470     ASN B  80    ND2
REMARK 470     THR B  88    CG2
REMARK 470     MET B  91    CE
REMARK 470     LEU B 100    CD1  CD2
REMARK 470     GLY B 101    CA   O
REMARK 470     HIS B 102    CG   ND1  CD2  CE1  NE2
REMARK 470     LYS B 104    CE   NZ
REMARK 470     GLU B 122    CB   CG   CD   OE1  OE2
REMARK 470     ARG B 123    NH1  NH2
REMARK 470     ILE B 131    CD1
REMARK 470     LEU B 146    CD1  CD2
REMARK 470     VAL B 149    CG1  CG2
REMARK 470     ALA B 151    CB
REMARK 470     LYS B 155    CG   CD   CE   NZ
REMARK 470     GLU B 157    CB   CG   CD   OE1  OE2
REMARK 470     LEU B 159    CB   CG   CD1  CD2
REMARK 470     ALA B 160    CB
REMARK 470     LEU B 162    CD1  CD2
REMARK 470     MET B 163    CE
REMARK 470     ARG B 168    O    CB   CG   CD   NE   CZ   NH1
REMARK 470     ARG B 168    NH2
REMARK 470     VAL B 169    CG1  CG2
REMARK 470     LEU B 173    CD1  CD2
REMARK 470     ILE B 175    CG2
REMARK 470     ARG B 178    CG   CD   NE   CZ   NH1  NH2
REMARK 470     ASP B 181    OD2
REMARK 470     ALA B 182    CB
REMARK 470     ARG B 185    NH1  NH2
REMARK 470     LEU B 187    CD2
REMARK 470     ARG B 190    CD   NE   CZ   NH1  NH2
REMARK 470     LEU B 192    CD1
REMARK 470     GLU B 202    OE1  OE2
REMARK 470     MET B 204    CE
REMARK 470     VAL B 212    CG1
REMARK 470     ALA B 225    CB
REMARK 470     ALA B 226    CB
REMARK 470     ARG B 227    CB   CG   CD   NE   CZ   NH1  NH2
REMARK 470     ASP B 232    OD1
REMARK 470     VAL B 243    CG1
REMARK 470     VAL B 260    CB   CG1  CG2
REMARK 470     PRO B 261    CB   CG
REMARK 470     ASP B 271    OD1  OD2
REMARK 470     VAL B 289    CG1  CG2
REMARK 470     LYS B 293    CE   NZ
REMARK 470     HIS C  13    N
REMARK 470     VAL C  15    CG1  CG2
REMARK 470     PHE C  16    CD1
REMARK 470     GLU C  38    CG   CD   OE1  OE2
REMARK 470     LEU C  42    CD1  CD2
REMARK 470     ILE C  44    CD1
REMARK 470     GLU C  63    OE1  OE2
REMARK 470     LYS C  68    NZ
REMARK 470     ASP C  69    OD2
REMARK 470     ASP C  84    OD2
REMARK 470     THR C  88    CG2
REMARK 470     MET C  91    CG   SD   CE
REMARK 470     GLU C  92    CG   CD   OE1  OE2
REMARK 470     TYR C  98    CD1  CD2  CE1  CE2  CZ   OH
REMARK 470     LEU C 100    CG   CD1  CD2
REMARK 470     HIS C 102    CG   ND1  CD2  CE1  NE2
REMARK 470     GLY C 103    O
REMARK 470     LYS C 104    NZ
REMARK 470     VAL C 105    CG1
REMARK 470     VAL C 106    CG2
REMARK 470     LEU C 107    CD1  CD2
REMARK 470     ALA C 121    CB
REMARK 470     GLU C 122    OE2
REMARK 470     ARG C 123    CB   CG   CD   NE   CZ   NH1  NH2
REMARK 470     PRO C 125    CB
REMARK 470     GLU C 126    CB   CG   CD   OE1  OE2
REMARK 470     LYS C 127    CG   CD   CE   NZ
REMARK 470     ALA C 129    CB
REMARK 470     LYS C 130    CB   CG   CD   CE   NZ
REMARK 470     ILE C 131    CD1
REMARK 470     VAL C 132    CG1  CG2
REMARK 470     LEU C 134    CD1  CD2
REMARK 470     VAL C 149    CG1
REMARK 470     LYS C 155    CG   CD   CE   NZ
REMARK 470     GLU C 157    CG   CD   OE1  OE2
REMARK 470     LEU C 159    CD1  CD2
REMARK 470     ALA C 160    CB
REMARK 470     ARG C 168    CG   CD   NE   CZ   NH1  NH2
REMARK 470     LEU C 173    CD1  CD2
REMARK 470     ARG C 178    CZ   NH1  NH2
REMARK 470     LEU C 187    CD1
REMARK 470     GLU C 202    OE1  OE2
REMARK 470     MET C 204    CE
REMARK 470     ILE C 221    CD1
REMARK 470     PRO C 222    CD
REMARK 470     THR C 223    CG2
REMARK 470     THR C 224    CB   OG1  CG2
REMARK 470     ALA C 225    CB
REMARK 470     ARG C 227    CD   NE   CZ   NH1  NH2
REMARK 470     TRP C 228    CG   CD1  CD2  NE1  CE2  CE3  CZ2
REMARK 470     TRP C 228    CZ3  CH2
REMARK 470     ALA C 230    O    CB
REMARK 470     ILE C 231    CB   CG1  CG2  CD1
REMARK 470     ASP C 232    CB   CG   OD1  OD2
REMARK 470     ARG C 233    NE   CZ   NH1  NH2
REMARK 470     ARG C 242    CZ   NH1  NH2
REMARK 470     ILE C 244    CD1
REMARK 470     LEU C 245    CD1
REMARK 470     ALA C 256    CB
REMARK 470     ASP C 257    CB   CG   OD1  OD2
REMARK 470     PHE C 259    CD1  CD2  CE1  CE2  CZ
REMARK 470     VAL C 260    CG1  CG2
REMARK 470     PRO C 261    CG   CD
REMARK 470     ASN C 263    CG   OD1  ND2
REMARK 470     PRO C 264    CG
REMARK 470     THR C 265    CG2
REMARK 470     VAL C 267    CG1  CG2
REMARK 470     GLN C 269    NE2
REMARK 470     LEU C 270    CD1
REMARK 470     VAL C 283    CG1
REMARK 470     VAL C 289    CG1  CG2
REMARK 470     LYS C 293    NZ
REMARK 470     SER C 294    OG
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ARG A 233   NE  -  CZ  -  NH1 ANGL. DEV. =   5.9 DEGREES
REMARK 500    ARG A 233   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.6 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A 111     -127.53     54.77
REMARK 500    TYR A 193       41.56   -143.44
REMARK 500    ARG A 227      -84.39   -108.04
REMARK 500    SER A 273     -172.98    -69.20
REMARK 500    SER A 275       63.90   -118.88
REMARK 500    GLN A 280       57.03   -146.94
REMARK 500    TRP B  23       -0.39     71.20
REMARK 500    SER B 111     -125.12     58.02
REMARK 500    ALA B 124       77.55   -157.44
REMARK 500    TYR B 193       38.51   -144.41
REMARK 500    ARG B 227      -83.38   -100.94
REMARK 500    SER B 275       64.31   -117.38
REMARK 500    SER C 111     -126.04     52.47
REMARK 500    ALA C 124       85.61   -164.70
REMARK 500    TYR C 193       39.36   -145.36
REMARK 500    SER C 273     -176.54    -68.64
REMARK 500    SER C 275       69.80   -118.41
REMARK 500    GLN C 280       47.86   -143.74
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A 567        DISTANCE =  6.29 ANGSTROMS
REMARK 525    HOH C 482        DISTANCE =  6.51 ANGSTROMS
DBREF1 8HFW A    1   294  UNP                  A0A4V2WUI0_BURPY
DBREF2 8HFW A     A0A4V2WUI0                          1         294
DBREF1 8HFW B    1   294  UNP                  A0A4V2WUI0_BURPY
DBREF2 8HFW B     A0A4V2WUI0                          1         294
DBREF1 8HFW C    1   294  UNP                  A0A4V2WUI0_BURPY
DBREF2 8HFW C     A0A4V2WUI0                          1         294
SEQADV 8HFW VAL A    5  UNP  A0A4V2WUI ILE     5 CONFLICT
SEQADV 8HFW ALA A    8  UNP  A0A4V2WUI PRO     8 CONFLICT
SEQADV 8HFW ALA A    9  UNP  A0A4V2WUI GLN     9 CONFLICT
SEQADV 8HFW PRO A   10  UNP  A0A4V2WUI GLN    10 CONFLICT
SEQADV 8HFW SER A   11  UNP  A0A4V2WUI ALA    11 CONFLICT
SEQADV 8HFW GLU A   38  UNP  A0A4V2WUI ALA    38 CONFLICT
SEQADV 8HFW GLU A   92  UNP  A0A4V2WUI GLN    92 CONFLICT
SEQADV 8HFW HIS A  102  UNP  A0A4V2WUI ARG   102 CONFLICT
SEQADV 8HFW ALA A  160  UNP  A0A4V2WUI GLY   160 CONFLICT
SEQADV 8HFW PRO A  161  UNP  A0A4V2WUI ALA   161 CONFLICT
SEQADV 8HFW ALA A  186  UNP  A0A4V2WUI ASP   186 CONFLICT
SEQADV 8HFW LEU A  187  UNP  A0A4V2WUI THR   187 CONFLICT
SEQADV 8HFW ALA A  188  UNP  A0A4V2WUI MET   188 CONFLICT
SEQADV 8HFW ALA A  197  UNP  A0A4V2WUI PRO   197 CONFLICT
SEQADV 8HFW MET A  204  UNP  A0A4V2WUI VAL   204 CONFLICT
SEQADV 8HFW MET A  208  UNP  A0A4V2WUI LEU   208 CONFLICT
SEQADV 8HFW THR A  219  UNP  A0A4V2WUI SER   219 CONFLICT
SEQADV 8HFW ALA A  226  UNP  A0A4V2WUI VAL   226 CONFLICT
SEQADV 8HFW ALA A  240  UNP  A0A4V2WUI GLN   240 CONFLICT
SEQADV 8HFW VAL A  260  UNP  A0A4V2WUI ALA   260 CONFLICT
SEQADV 8HFW GLN A  280  UNP  A0A4V2WUI GLU   280 CONFLICT
SEQADV 8HFW PRO A  281  UNP  A0A4V2WUI ALA   281 CONFLICT
SEQADV 8HFW GLY A  282  UNP  A0A4V2WUI ALA   282 CONFLICT
SEQADV 8HFW VAL A  289  UNP  A0A4V2WUI ALA   289 CONFLICT
SEQADV 8HFW ILE A  295  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW ALA A  296  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW LEU A  297  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW GLU A  298  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS A  299  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS A  300  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS A  301  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS A  302  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS A  303  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS A  304  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW VAL B    5  UNP  A0A4V2WUI ILE     5 CONFLICT
SEQADV 8HFW ALA B    8  UNP  A0A4V2WUI PRO     8 CONFLICT
SEQADV 8HFW ALA B    9  UNP  A0A4V2WUI GLN     9 CONFLICT
SEQADV 8HFW PRO B   10  UNP  A0A4V2WUI GLN    10 CONFLICT
SEQADV 8HFW SER B   11  UNP  A0A4V2WUI ALA    11 CONFLICT
SEQADV 8HFW GLU B   38  UNP  A0A4V2WUI ALA    38 CONFLICT
SEQADV 8HFW GLU B   92  UNP  A0A4V2WUI GLN    92 CONFLICT
SEQADV 8HFW HIS B  102  UNP  A0A4V2WUI ARG   102 CONFLICT
SEQADV 8HFW ALA B  160  UNP  A0A4V2WUI GLY   160 CONFLICT
SEQADV 8HFW PRO B  161  UNP  A0A4V2WUI ALA   161 CONFLICT
SEQADV 8HFW ALA B  186  UNP  A0A4V2WUI ASP   186 CONFLICT
SEQADV 8HFW LEU B  187  UNP  A0A4V2WUI THR   187 CONFLICT
SEQADV 8HFW ALA B  188  UNP  A0A4V2WUI MET   188 CONFLICT
SEQADV 8HFW ALA B  197  UNP  A0A4V2WUI PRO   197 CONFLICT
SEQADV 8HFW MET B  204  UNP  A0A4V2WUI VAL   204 CONFLICT
SEQADV 8HFW MET B  208  UNP  A0A4V2WUI LEU   208 CONFLICT
SEQADV 8HFW THR B  219  UNP  A0A4V2WUI SER   219 CONFLICT
SEQADV 8HFW ALA B  226  UNP  A0A4V2WUI VAL   226 CONFLICT
SEQADV 8HFW ALA B  240  UNP  A0A4V2WUI GLN   240 CONFLICT
SEQADV 8HFW VAL B  260  UNP  A0A4V2WUI ALA   260 CONFLICT
SEQADV 8HFW GLN B  280  UNP  A0A4V2WUI GLU   280 CONFLICT
SEQADV 8HFW PRO B  281  UNP  A0A4V2WUI ALA   281 CONFLICT
SEQADV 8HFW GLY B  282  UNP  A0A4V2WUI ALA   282 CONFLICT
SEQADV 8HFW VAL B  289  UNP  A0A4V2WUI ALA   289 CONFLICT
SEQADV 8HFW ILE B  295  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW ALA B  296  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW LEU B  297  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW GLU B  298  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS B  299  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS B  300  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS B  301  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS B  302  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS B  303  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS B  304  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW VAL C    5  UNP  A0A4V2WUI ILE     5 CONFLICT
SEQADV 8HFW ALA C    8  UNP  A0A4V2WUI PRO     8 CONFLICT
SEQADV 8HFW ALA C    9  UNP  A0A4V2WUI GLN     9 CONFLICT
SEQADV 8HFW PRO C   10  UNP  A0A4V2WUI GLN    10 CONFLICT
SEQADV 8HFW SER C   11  UNP  A0A4V2WUI ALA    11 CONFLICT
SEQADV 8HFW GLU C   38  UNP  A0A4V2WUI ALA    38 CONFLICT
SEQADV 8HFW GLU C   92  UNP  A0A4V2WUI GLN    92 CONFLICT
SEQADV 8HFW HIS C  102  UNP  A0A4V2WUI ARG   102 CONFLICT
SEQADV 8HFW ALA C  160  UNP  A0A4V2WUI GLY   160 CONFLICT
SEQADV 8HFW PRO C  161  UNP  A0A4V2WUI ALA   161 CONFLICT
SEQADV 8HFW ALA C  186  UNP  A0A4V2WUI ASP   186 CONFLICT
SEQADV 8HFW LEU C  187  UNP  A0A4V2WUI THR   187 CONFLICT
SEQADV 8HFW ALA C  188  UNP  A0A4V2WUI MET   188 CONFLICT
SEQADV 8HFW ALA C  197  UNP  A0A4V2WUI PRO   197 CONFLICT
SEQADV 8HFW MET C  204  UNP  A0A4V2WUI VAL   204 CONFLICT
SEQADV 8HFW MET C  208  UNP  A0A4V2WUI LEU   208 CONFLICT
SEQADV 8HFW THR C  219  UNP  A0A4V2WUI SER   219 CONFLICT
SEQADV 8HFW ALA C  226  UNP  A0A4V2WUI VAL   226 CONFLICT
SEQADV 8HFW ALA C  240  UNP  A0A4V2WUI GLN   240 CONFLICT
SEQADV 8HFW VAL C  260  UNP  A0A4V2WUI ALA   260 CONFLICT
SEQADV 8HFW GLN C  280  UNP  A0A4V2WUI GLU   280 CONFLICT
SEQADV 8HFW PRO C  281  UNP  A0A4V2WUI ALA   281 CONFLICT
SEQADV 8HFW GLY C  282  UNP  A0A4V2WUI ALA   282 CONFLICT
SEQADV 8HFW VAL C  289  UNP  A0A4V2WUI ALA   289 CONFLICT
SEQADV 8HFW ILE C  295  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW ALA C  296  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW LEU C  297  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW GLU C  298  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS C  299  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS C  300  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS C  301  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS C  302  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS C  303  UNP  A0A4V2WUI           EXPRESSION TAG
SEQADV 8HFW HIS C  304  UNP  A0A4V2WUI           EXPRESSION TAG
SEQRES   1 A  304  MET GLU THR ASN VAL THR ALA ALA ALA PRO SER ASP HIS
SEQRES   2 A  304  PRO VAL PHE VAL LEU VAL HIS GLY ALA TRP HIS GLY ALA
SEQRES   3 A  304  TRP CYS TYR ALA HIS VAL ALA ALA ALA LEU ALA GLU ARG
SEQRES   4 A  304  GLY TYR LEU SER ILE ALA ARG ASP LEU PRO ALA HIS GLY
SEQRES   5 A  304  ILE ASN ALA ARG PHE PRO ALA SER TYR LEU GLU ARG PRO
SEQRES   6 A  304  LEU ASP LYS ASP ALA PHE GLY ALA GLU PRO SER PRO VAL
SEQRES   7 A  304  ALA ASN THR THR LEU ASP ASP TYR ALA THR GLN VAL MET
SEQRES   8 A  304  GLU ALA VAL ASP ASP ALA TYR ALA LEU GLY HIS GLY LYS
SEQRES   9 A  304  VAL VAL LEU VAL GLY HIS SER MET GLY GLY LEU ALA ILE
SEQRES  10 A  304  THR ALA ALA ALA GLU ARG ALA PRO GLU LYS ILE ALA LYS
SEQRES  11 A  304  ILE VAL TYR LEU ALA ALA PHE MET PRO ALA SER GLY VAL
SEQRES  12 A  304  PRO GLY LEU ASP TYR VAL ARG ALA PRO GLU ASN LYS GLY
SEQRES  13 A  304  GLU MET LEU ALA PRO LEU MET LEU ALA SER PRO ARG VAL
SEQRES  14 A  304  ALA GLY ALA LEU ARG ILE ASP PRO ARG SER GLY ASP ALA
SEQRES  15 A  304  ALA TYR ARG ALA LEU ALA LYS ARG ALA LEU TYR ASP ASP
SEQRES  16 A  304  ALA ALA GLN ALA ASP PHE GLU ALA MET ALA ASN LEU MET
SEQRES  17 A  304  THR CYS ASP VAL PRO ALA ALA PRO PHE ALA THR ALA ILE
SEQRES  18 A  304  PRO THR THR ALA ALA ARG TRP GLY ALA ILE ASP ARG HIS
SEQRES  19 A  304  TYR ILE LYS CYS LEU ALA ASP ARG VAL ILE LEU PRO ALA
SEQRES  20 A  304  LEU GLN GLN ARG PHE ILE ASP GLU ALA ASP ALA PHE VAL
SEQRES  21 A  304  PRO GLY ASN PRO THR HIS VAL HIS GLN LEU ASP SER SER
SEQRES  22 A  304  HIS SER PRO PHE VAL SER GLN PRO GLY VAL LEU ALA GLY
SEQRES  23 A  304  VAL LEU VAL ASP ILE ALA LYS SER ILE ALA LEU GLU HIS
SEQRES  24 A  304  HIS HIS HIS HIS HIS
SEQRES   1 B  304  MET GLU THR ASN VAL THR ALA ALA ALA PRO SER ASP HIS
SEQRES   2 B  304  PRO VAL PHE VAL LEU VAL HIS GLY ALA TRP HIS GLY ALA
SEQRES   3 B  304  TRP CYS TYR ALA HIS VAL ALA ALA ALA LEU ALA GLU ARG
SEQRES   4 B  304  GLY TYR LEU SER ILE ALA ARG ASP LEU PRO ALA HIS GLY
SEQRES   5 B  304  ILE ASN ALA ARG PHE PRO ALA SER TYR LEU GLU ARG PRO
SEQRES   6 B  304  LEU ASP LYS ASP ALA PHE GLY ALA GLU PRO SER PRO VAL
SEQRES   7 B  304  ALA ASN THR THR LEU ASP ASP TYR ALA THR GLN VAL MET
SEQRES   8 B  304  GLU ALA VAL ASP ASP ALA TYR ALA LEU GLY HIS GLY LYS
SEQRES   9 B  304  VAL VAL LEU VAL GLY HIS SER MET GLY GLY LEU ALA ILE
SEQRES  10 B  304  THR ALA ALA ALA GLU ARG ALA PRO GLU LYS ILE ALA LYS
SEQRES  11 B  304  ILE VAL TYR LEU ALA ALA PHE MET PRO ALA SER GLY VAL
SEQRES  12 B  304  PRO GLY LEU ASP TYR VAL ARG ALA PRO GLU ASN LYS GLY
SEQRES  13 B  304  GLU MET LEU ALA PRO LEU MET LEU ALA SER PRO ARG VAL
SEQRES  14 B  304  ALA GLY ALA LEU ARG ILE ASP PRO ARG SER GLY ASP ALA
SEQRES  15 B  304  ALA TYR ARG ALA LEU ALA LYS ARG ALA LEU TYR ASP ASP
SEQRES  16 B  304  ALA ALA GLN ALA ASP PHE GLU ALA MET ALA ASN LEU MET
SEQRES  17 B  304  THR CYS ASP VAL PRO ALA ALA PRO PHE ALA THR ALA ILE
SEQRES  18 B  304  PRO THR THR ALA ALA ARG TRP GLY ALA ILE ASP ARG HIS
SEQRES  19 B  304  TYR ILE LYS CYS LEU ALA ASP ARG VAL ILE LEU PRO ALA
SEQRES  20 B  304  LEU GLN GLN ARG PHE ILE ASP GLU ALA ASP ALA PHE VAL
SEQRES  21 B  304  PRO GLY ASN PRO THR HIS VAL HIS GLN LEU ASP SER SER
SEQRES  22 B  304  HIS SER PRO PHE VAL SER GLN PRO GLY VAL LEU ALA GLY
SEQRES  23 B  304  VAL LEU VAL ASP ILE ALA LYS SER ILE ALA LEU GLU HIS
SEQRES  24 B  304  HIS HIS HIS HIS HIS
SEQRES   1 C  304  MET GLU THR ASN VAL THR ALA ALA ALA PRO SER ASP HIS
SEQRES   2 C  304  PRO VAL PHE VAL LEU VAL HIS GLY ALA TRP HIS GLY ALA
SEQRES   3 C  304  TRP CYS TYR ALA HIS VAL ALA ALA ALA LEU ALA GLU ARG
SEQRES   4 C  304  GLY TYR LEU SER ILE ALA ARG ASP LEU PRO ALA HIS GLY
SEQRES   5 C  304  ILE ASN ALA ARG PHE PRO ALA SER TYR LEU GLU ARG PRO
SEQRES   6 C  304  LEU ASP LYS ASP ALA PHE GLY ALA GLU PRO SER PRO VAL
SEQRES   7 C  304  ALA ASN THR THR LEU ASP ASP TYR ALA THR GLN VAL MET
SEQRES   8 C  304  GLU ALA VAL ASP ASP ALA TYR ALA LEU GLY HIS GLY LYS
SEQRES   9 C  304  VAL VAL LEU VAL GLY HIS SER MET GLY GLY LEU ALA ILE
SEQRES  10 C  304  THR ALA ALA ALA GLU ARG ALA PRO GLU LYS ILE ALA LYS
SEQRES  11 C  304  ILE VAL TYR LEU ALA ALA PHE MET PRO ALA SER GLY VAL
SEQRES  12 C  304  PRO GLY LEU ASP TYR VAL ARG ALA PRO GLU ASN LYS GLY
SEQRES  13 C  304  GLU MET LEU ALA PRO LEU MET LEU ALA SER PRO ARG VAL
SEQRES  14 C  304  ALA GLY ALA LEU ARG ILE ASP PRO ARG SER GLY ASP ALA
SEQRES  15 C  304  ALA TYR ARG ALA LEU ALA LYS ARG ALA LEU TYR ASP ASP
SEQRES  16 C  304  ALA ALA GLN ALA ASP PHE GLU ALA MET ALA ASN LEU MET
SEQRES  17 C  304  THR CYS ASP VAL PRO ALA ALA PRO PHE ALA THR ALA ILE
SEQRES  18 C  304  PRO THR THR ALA ALA ARG TRP GLY ALA ILE ASP ARG HIS
SEQRES  19 C  304  TYR ILE LYS CYS LEU ALA ASP ARG VAL ILE LEU PRO ALA
SEQRES  20 C  304  LEU GLN GLN ARG PHE ILE ASP GLU ALA ASP ALA PHE VAL
SEQRES  21 C  304  PRO GLY ASN PRO THR HIS VAL HIS GLN LEU ASP SER SER
SEQRES  22 C  304  HIS SER PRO PHE VAL SER GLN PRO GLY VAL LEU ALA GLY
SEQRES  23 C  304  VAL LEU VAL ASP ILE ALA LYS SER ILE ALA LEU GLU HIS
SEQRES  24 C  304  HIS HIS HIS HIS HIS
FORMUL   4  HOH   *329(H2 O)
HELIX    1 AA1 GLY A   25  CYS A   28  5                                   4
HELIX    2 AA2 TYR A   29  ARG A   39  1                                  11
HELIX    3 AA3 HIS A   51  ALA A   55  5                                   5
HELIX    4 AA4 PRO A   58  LEU A   62  5                                   5
HELIX    5 AA5 ASP A   67  GLU A   74  1                                   8
HELIX    6 AA6 THR A   82  LEU A  100  1                                  19
HELIX    7 AA7 MET A  112  ALA A  124  1                                  13
HELIX    8 AA8 PRO A  144  ARG A  150  1                                   7
HELIX    9 AA9 ALA A  151  LYS A  155  5                                   5
HELIX   10 AB1 LEU A  159  MET A  163  5                                   5
HELIX   11 AB2 SER A  166  GLY A  171  1                                   6
HELIX   12 AB3 ASP A  181  TYR A  193  1                                  13
HELIX   13 AB4 ALA A  197  MET A  208  1                                  12
HELIX   14 AB5 ALA A  215  THR A  219  5                                   5
HELIX   15 AB6 ARG A  227  ILE A  231  5                                   5
HELIX   16 AB7 LEU A  245  VAL A  260  1                                  16
HELIX   17 AB8 SER A  275  GLN A  280  1                                   6
HELIX   18 AB9 GLN A  280  SER A  294  1                                  15
HELIX   19 AC1 GLY B   25  CYS B   28  5                                   4
HELIX   20 AC2 TYR B   29  ARG B   39  1                                  11
HELIX   21 AC3 HIS B   51  ALA B   55  5                                   5
HELIX   22 AC4 PRO B   58  GLU B   63  5                                   6
HELIX   23 AC5 ASP B   67  GLU B   74  1                                   8
HELIX   24 AC6 THR B   82  LEU B  100  1                                  19
HELIX   25 AC7 MET B  112  ALA B  124  1                                  13
HELIX   26 AC8 PRO B  144  VAL B  149  1                                   6
HELIX   27 AC9 ALA B  151  LYS B  155  5                                   5
HELIX   28 AD1 LEU B  159  MET B  163  5                                   5
HELIX   29 AD2 ASP B  181  TYR B  193  1                                  13
HELIX   30 AD3 ALA B  197  MET B  208  1                                  12
HELIX   31 AD4 ALA B  214  THR B  219  1                                   6
HELIX   32 AD5 ARG B  227  ILE B  231  5                                   5
HELIX   33 AD6 LEU B  245  VAL B  260  1                                  16
HELIX   34 AD7 SER B  275  GLN B  280  1                                   6
HELIX   35 AD8 GLN B  280  SER B  294  1                                  15
HELIX   36 AD9 GLY C   25  CYS C   28  5                                   4
HELIX   37 AE1 TYR C   29  ARG C   39  1                                  11
HELIX   38 AE2 HIS C   51  ALA C   55  5                                   5
HELIX   39 AE3 PRO C   58  GLU C   63  5                                   6
HELIX   40 AE4 ASP C   67  GLU C   74  1                                   8
HELIX   41 AE5 THR C   82  HIS C  102  1                                  21
HELIX   42 AE6 MET C  112  ALA C  124  1                                  13
HELIX   43 AE7 PRO C  144  ARG C  150  1                                   7
HELIX   44 AE8 ALA C  151  LYS C  155  5                                   5
HELIX   45 AE9 LEU C  159  MET C  163  5                                   5
HELIX   46 AF1 SER C  166  GLY C  171  1                                   6
HELIX   47 AF2 ASP C  181  TYR C  193  1                                  13
HELIX   48 AF3 ALA C  197  MET C  208  1                                  12
HELIX   49 AF4 ALA C  215  THR C  219  5                                   5
HELIX   50 AF5 LEU C  245  VAL C  260  1                                  16
HELIX   51 AF6 SER C  275  GLN C  280  1                                   6
HELIX   52 AF7 GLN C  280  SER C  294  1                                  15
SHEET    1 AA1 6 SER A  43  ALA A  45  0
SHEET    2 AA1 6 PHE A  16  VAL A  19  1  N  LEU A  18   O  ILE A  44
SHEET    3 AA1 6 VAL A 105  SER A 111  1  O  VAL A 108   N  VAL A  17
SHEET    4 AA1 6 ILE A 128  ALA A 136  1  O  VAL A 132   N  LEU A 107
SHEET    5 AA1 6 ASP A 232  CYS A 238  1  O  ILE A 236   N  TYR A 133
SHEET    6 AA1 6 HIS A 266  LEU A 270  1  O  HIS A 266   N  ARG A 233
SHEET    1 AA2 2 ALA A 172  ARG A 174  0
SHEET    2 AA2 2 ASP A 211  PRO A 213 -1  O  VAL A 212   N  LEU A 173
SHEET    1 AA3 6 SER B  43  ALA B  45  0
SHEET    2 AA3 6 PHE B  16  VAL B  19  1  N  PHE B  16   O  ILE B  44
SHEET    3 AA3 6 VAL B 105  SER B 111  1  O  VAL B 108   N  VAL B  17
SHEET    4 AA3 6 ILE B 128  ALA B 136  1  O  VAL B 132   N  LEU B 107
SHEET    5 AA3 6 ASP B 232  CYS B 238  1  O  ILE B 236   N  TYR B 133
SHEET    6 AA3 6 HIS B 266  LEU B 270  1  O  HIS B 268   N  LYS B 237
SHEET    1 AA4 2 ALA B 172  ARG B 174  0
SHEET    2 AA4 2 ASP B 211  PRO B 213 -1  O  VAL B 212   N  LEU B 173
SHEET    1 AA5 6 SER C  43  ALA C  45  0
SHEET    2 AA5 6 PHE C  16  VAL C  19  1  N  PHE C  16   O  ILE C  44
SHEET    3 AA5 6 VAL C 105  SER C 111  1  O  HIS C 110   N  VAL C  19
SHEET    4 AA5 6 ILE C 128  ALA C 136  1  O  LEU C 134   N  GLY C 109
SHEET    5 AA5 6 ASP C 232  CYS C 238  1  O  ILE C 236   N  TYR C 133
SHEET    6 AA5 6 HIS C 266  LEU C 270  1  O  HIS C 266   N  ARG C 233
SHEET    1 AA6 2 ALA C 172  ARG C 174  0
SHEET    2 AA6 2 ASP C 211  PRO C 213 -1  O  VAL C 212   N  LEU C 173
CISPEP   1 ARG A   64    PRO A   65          0        10.08
CISPEP   2 ARG B   64    PRO B   65          0         7.18
CISPEP   3 ARG C   64    PRO C   65          0        -7.75
CRYST1  166.960   68.410   75.340  90.00  93.58  90.00 C 1 2 1      12
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.005989  0.000000  0.000374        0.00000
SCALE2      0.000000  0.014618  0.000000        0.00000
SCALE3      0.000000  0.000000  0.013299        0.00000
TER    2078      LEU A 297
TER    4054      SER B 294
TER    5992      SER C 294
MASTER      605    0    0   52   24    0    0    6 6306    3    0   72
END