longtext: 8hm5-pdb

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HEADER    HYDROLASE                               02-DEC-22   8HM5
TITLE     EPOXIDE HYDROLASE FROM CABALLERONIA SORDIDICOLA PAMC 26510
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: EPOXIDE HYDROLASE;
COMPND   3 CHAIN: A, B;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: CABALLERONIA SORDIDICOLA;
SOURCE   3 ORGANISM_TAXID: 196367;
SOURCE   4 GENE: PAMC26510_32755;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    BACTERIAL EPOXIDE HYDROLASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    J.HWANG,M.J.LEE,H.DO,J.H.LEE
REVDAT   1   06-DEC-23 8HM5    0
JRNL        AUTH   J.HWANG,M.J.LEE,H.DO,J.H.LEE
JRNL        TITL   EPOXIDE HYDROLASE FROM CABALLERONIA SORDIDICOLA PAMC 26510
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.43 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.5
REMARK   3   AUTHORS     : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK   3               : NICHOLLS,WINN,LONG,VAGIN
REMARK   3
REMARK   3    REFINEMENT TARGET : CDL V1.2
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.43
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 43.41
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 1.390
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.4
REMARK   3   NUMBER OF REFLECTIONS             : 20329
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : FREE R-VALUE
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.173
REMARK   3   R VALUE            (WORKING SET) : 0.171
REMARK   3   FREE R VALUE                     : 0.217
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.790
REMARK   3   FREE R VALUE TEST SET COUNT      : 974
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.43
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.56
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 2673
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 93.98
REMARK   3   BIN R VALUE           (WORKING SET) : 0.1913
REMARK   3   BIN FREE R VALUE SET COUNT          : 123
REMARK   3   BIN FREE R VALUE                    : 0.2540
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 4942
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 0
REMARK   3   SOLVENT ATOMS            : 182
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 20.68
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): NULL
REMARK   3   ESU BASED ON FREE R VALUE                       (A): NULL
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.226
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): NULL
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : NULL
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : NULL
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  NULL ;  NULL ;  NULL
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  NULL ;  NULL ;  NULL
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : FLAT BULK SOLVENT MODEL
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.11
REMARK   3   ION PROBE RADIUS   : NULL
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 8HM5 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 13-DEC-22.
REMARK 100 THE DEPOSITION ID IS D_1300033749.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 05-AUG-20
REMARK 200  TEMPERATURE           (KELVIN) : 113
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : N
REMARK 200  RADIATION SOURCE               : ROTATING ANODE
REMARK 200  BEAMLINE                       : NULL
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-007 HF
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418
REMARK 200  MONOCHROMATOR                  : CONFOCAL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU RAXIS IV
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XIA2
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 20365
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.430
REMARK 200  RESOLUTION RANGE LOW       (A) : 81.210
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.7
REMARK 200  DATA REDUNDANCY                : 4.500
REMARK 200  R MERGE                    (I) : 0.07000
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 10.5000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.43
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.56
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 0.17700
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 6.100
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 39.43
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.03
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M MAGNESIUM FORMATE AND 20% (W/V)
REMARK 280  PEG 3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 296K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       40.60500
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     THR A     2
REMARK 465     SER A     3
REMARK 465     ASP A     4
REMARK 465     ILE A     5
REMARK 465     THR A     6
REMARK 465     LEU A   323
REMARK 465     SER A   324
REMARK 465     MET B     1
REMARK 465     THR B     2
REMARK 465     SER B     3
REMARK 465     ASP B     4
REMARK 465     ILE B     5
REMARK 465     THR B     6
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    GLU A 310   CB  -  CA  -  C   ANGL. DEV. =  12.6 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASP A 106     -129.22     62.14
REMARK 500    SER A 130      -45.48     70.58
REMARK 500    TRP A 299       57.85    -90.98
REMARK 500    ASP B 106     -126.69     63.74
REMARK 500    SER B 130      -51.80     73.51
REMARK 500    PRO B 206       53.19    -92.85
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: MAIN CHAIN PLANARITY
REMARK 500
REMARK 500 THE FOLLOWING RESIDUES HAVE A PSEUDO PLANARITY
REMARK 500 TORSION ANGLE, C(I) - CA(I) - N(I+1) - O(I), GREATER
REMARK 500 10.0 DEGREES. (M=MODEL NUMBER; RES=RESIDUE NAME;
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 500 I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        ANGLE
REMARK 500    LEU A 277        -13.43
REMARK 500
REMARK 500 REMARK: NULL
DBREF1 8HM5 A    1   324  UNP                  A0A242M8J4_9BURK
DBREF2 8HM5 A     A0A242M8J4                          6         329
DBREF1 8HM5 B    1   324  UNP                  A0A242M8J4_9BURK
DBREF2 8HM5 B     A0A242M8J4                          6         329
SEQRES   1 A  324  MET THR SER ASP ILE THR GLY GLY VAL LYS GLU TYR ASP
SEQRES   2 A  324  ILE ALA THR ASN GLY ILE SER LEU HIS VAL THR GLU GLN
SEQRES   3 A  324  GLY ALA GLY PRO ALA VAL LEU PHE CYS HIS GLY PHE PRO
SEQRES   4 A  324  ASP THR SER TYR THR TRP ARG ARG GLN MET ASN ALA ILE
SEQRES   5 A  324  ALA SER ALA GLY TYR ARG ALA ILE ALA PRO ASP MET ARG
SEQRES   6 A  324  GLY TYR GLY ARG SER SER ALA PRO ALA ASP ALA SER LEU
SEQRES   7 A  324  TYR THR PRO LEU HIS THR THR GLY ASP LEU ILE GLY LEU
SEQRES   8 A  324  LEU ASP ALA LEU LYS ILE SER SER ALA VAL LEU VAL GLY
SEQRES   9 A  324  HIS ASP TRP GLY ALA THR HIS ALA TRP ASN ALA ALA LEU
SEQRES  10 A  324  MET ARG PRO ASP ARG PHE LYS ALA VAL PHE GLY LEU SER
SEQRES  11 A  324  VAL PRO PHE VAL THR ARG GLY GLU SER SER VAL PHE GLU
SEQRES  12 A  324  ARG MET ARG GLU SER GLY ARG GLN ASP ASP PHE TYR MET
SEQRES  13 A  324  PHE GLU GLN ILE ARG PRO ASP ALA ASP GLN ILE TRP ALA
SEQRES  14 A  324  ASP ALA ALA VAL THR ILE PRO GLY ILE LEU TYR TRP ALA
SEQRES  15 A  324  SER GLY SER ALA PRO GLU GLY GLU GLN TRP SER PRO LEU
SEQRES  16 A  324  ASP ARG THR ARG SER LEU TYR ARG ALA ALA PRO GLY PRO
SEQRES  17 A  324  LEU PRO SER TRP ALA GLU ALA ASP TYR VAL ALA HIS ASN
SEQRES  18 A  324  ILE ALA GLU PHE ARG ARG THR GLY PHE HIS GLY GLY LEU
SEQRES  19 A  324  ASN TYR TYR ARG ALA ALA GLU PRO TYR PHE THR LEU SER
SEQRES  20 A  324  ALA PRO TRP LYS GLY ALA LYS ILE THR GLN PRO SER PHE
SEQRES  21 A  324  PHE ILE TRP GLY LYS SER ASP GLY LEU LYS GLU LEU TYR
SEQRES  22 A  324  PRO PHE THR LEU GLN GLN MET ARG ALA GLY LEU PRO GLY
SEQRES  23 A  324  LEU MET GLY GLY LEU GLU LEU ASP ASN VAL GLY HIS TRP
SEQRES  24 A  324  VAL GLN HIS GLU ALA SER ALA GLU VAL SER GLU GLN LEU
SEQRES  25 A  324  VAL ARG PHE LEU ARG THR VAL ASP ALA VAL LEU SER
SEQRES   1 B  324  MET THR SER ASP ILE THR GLY GLY VAL LYS GLU TYR ASP
SEQRES   2 B  324  ILE ALA THR ASN GLY ILE SER LEU HIS VAL THR GLU GLN
SEQRES   3 B  324  GLY ALA GLY PRO ALA VAL LEU PHE CYS HIS GLY PHE PRO
SEQRES   4 B  324  ASP THR SER TYR THR TRP ARG ARG GLN MET ASN ALA ILE
SEQRES   5 B  324  ALA SER ALA GLY TYR ARG ALA ILE ALA PRO ASP MET ARG
SEQRES   6 B  324  GLY TYR GLY ARG SER SER ALA PRO ALA ASP ALA SER LEU
SEQRES   7 B  324  TYR THR PRO LEU HIS THR THR GLY ASP LEU ILE GLY LEU
SEQRES   8 B  324  LEU ASP ALA LEU LYS ILE SER SER ALA VAL LEU VAL GLY
SEQRES   9 B  324  HIS ASP TRP GLY ALA THR HIS ALA TRP ASN ALA ALA LEU
SEQRES  10 B  324  MET ARG PRO ASP ARG PHE LYS ALA VAL PHE GLY LEU SER
SEQRES  11 B  324  VAL PRO PHE VAL THR ARG GLY GLU SER SER VAL PHE GLU
SEQRES  12 B  324  ARG MET ARG GLU SER GLY ARG GLN ASP ASP PHE TYR MET
SEQRES  13 B  324  PHE GLU GLN ILE ARG PRO ASP ALA ASP GLN ILE TRP ALA
SEQRES  14 B  324  ASP ALA ALA VAL THR ILE PRO GLY ILE LEU TYR TRP ALA
SEQRES  15 B  324  SER GLY SER ALA PRO GLU GLY GLU GLN TRP SER PRO LEU
SEQRES  16 B  324  ASP ARG THR ARG SER LEU TYR ARG ALA ALA PRO GLY PRO
SEQRES  17 B  324  LEU PRO SER TRP ALA GLU ALA ASP TYR VAL ALA HIS ASN
SEQRES  18 B  324  ILE ALA GLU PHE ARG ARG THR GLY PHE HIS GLY GLY LEU
SEQRES  19 B  324  ASN TYR TYR ARG ALA ALA GLU PRO TYR PHE THR LEU SER
SEQRES  20 B  324  ALA PRO TRP LYS GLY ALA LYS ILE THR GLN PRO SER PHE
SEQRES  21 B  324  PHE ILE TRP GLY LYS SER ASP GLY LEU LYS GLU LEU TYR
SEQRES  22 B  324  PRO PHE THR LEU GLN GLN MET ARG ALA GLY LEU PRO GLY
SEQRES  23 B  324  LEU MET GLY GLY LEU GLU LEU ASP ASN VAL GLY HIS TRP
SEQRES  24 B  324  VAL GLN HIS GLU ALA SER ALA GLU VAL SER GLU GLN LEU
SEQRES  25 B  324  VAL ARG PHE LEU ARG THR VAL ASP ALA VAL LEU SER
FORMUL   3  HOH   *182(H2 O)
HELIX    1 AA1 THR A   41  THR A   44  5                                   4
HELIX    2 AA2 TRP A   45  ALA A   55  1                                  11
HELIX    3 AA3 ASP A   75  TYR A   79  5                                   5
HELIX    4 AA4 THR A   80  LEU A   95  1                                  16
HELIX    5 AA5 ASP A  106  ARG A  119  1                                  14
HELIX    6 AA6 SER A  140  SER A  148  1                                   9
HELIX    7 AA7 PHE A  154  ARG A  161  1                                   8
HELIX    8 AA8 ASP A  163  ALA A  169  1                                   7
HELIX    9 AA9 ASP A  170  ALA A  182  1                                  13
HELIX   10 AB1 GLU A  214  GLY A  229  1                                  16
HELIX   11 AB2 PHE A  230  SER A  247  1                                  18
HELIX   12 AB3 ALA A  248  LYS A  251  5                                   4
HELIX   13 AB4 ASP A  267  TYR A  273  5                                   7
HELIX   14 AB5 THR A  276  ARG A  281  1                                   6
HELIX   15 AB6 TRP A  299  ALA A  304  1                                   6
HELIX   16 AB7 ALA A  304  VAL A  322  1                                  19
HELIX   17 AB8 THR B   41  THR B   44  5                                   4
HELIX   18 AB9 TRP B   45  SER B   54  1                                  10
HELIX   19 AC1 ASP B   75  TYR B   79  5                                   5
HELIX   20 AC2 THR B   80  LEU B   95  1                                  16
HELIX   21 AC3 ASP B  106  ARG B  119  1                                  14
HELIX   22 AC4 SER B  140  SER B  148  1                                   9
HELIX   23 AC5 PHE B  154  ARG B  161  1                                   8
HELIX   24 AC6 ASP B  163  ALA B  169  1                                   7
HELIX   25 AC7 ASP B  170  ALA B  182  1                                  13
HELIX   26 AC8 GLU B  214  GLY B  229  1                                  16
HELIX   27 AC9 PHE B  230  SER B  247  1                                  18
HELIX   28 AD1 ALA B  248  LYS B  251  5                                   4
HELIX   29 AD2 ASP B  267  TYR B  273  5                                   7
HELIX   30 AD3 TRP B  299  ALA B  304  1                                   6
HELIX   31 AD4 ALA B  304  SER B  324  1                                  21
SHEET    1 AA1 8 LYS A  10  THR A  16  0
SHEET    2 AA1 8 ILE A  19  GLN A  26 -1  O  VAL A  23   N  TYR A  12
SHEET    3 AA1 8 ARG A  58  PRO A  62 -1  O  ALA A  59   N  GLN A  26
SHEET    4 AA1 8 ALA A  31  CYS A  35  1  N  PHE A  34   O  ILE A  60
SHEET    5 AA1 8 ALA A 100  HIS A 105  1  O  VAL A 101   N  LEU A  33
SHEET    6 AA1 8 PHE A 123  LEU A 129  1  O  ALA A 125   N  LEU A 102
SHEET    7 AA1 8 SER A 259  GLY A 264  1  O  PHE A 260   N  GLY A 128
SHEET    8 AA1 8 LEU A 287  LEU A 293  1  O  MET A 288   N  SER A 259
SHEET    1 AA2 8 LYS B  10  THR B  16  0
SHEET    2 AA2 8 ILE B  19  GLY B  27 -1  O  LEU B  21   N  ILE B  14
SHEET    3 AA2 8 ARG B  58  PRO B  62 -1  O  ALA B  61   N  THR B  24
SHEET    4 AA2 8 ALA B  31  CYS B  35  1  N  VAL B  32   O  ILE B  60
SHEET    5 AA2 8 ALA B 100  HIS B 105  1  O  VAL B 103   N  CYS B  35
SHEET    6 AA2 8 PHE B 123  LEU B 129  1  O  PHE B 127   N  LEU B 102
SHEET    7 AA2 8 SER B 259  GLY B 264  1  O  PHE B 260   N  GLY B 128
SHEET    8 AA2 8 LEU B 287  LEU B 293  1  O  MET B 288   N  SER B 259
CISPEP   1 PHE A   38    PRO A   39          0       -26.02
CISPEP   2 PHE B   38    PRO B   39          0       -23.13
CRYST1   43.470   81.210   79.830  90.00  93.11  90.00 P 1 21 1      4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.023004  0.000000  0.001250        0.00000
SCALE2      0.000000  0.012314  0.000000        0.00000
SCALE3      0.000000  0.000000  0.012545        0.00000
TER    2465      VAL A 322
TER    4944      SER B 324
MASTER      302    0    0   31   16    0    0    6 5124    2    0   50
END