longtext: 8ilt-pdb

content
HEADER    HYDROLASE                               04-MAR-23   8ILT
TITLE     CRYSTAL STRUCTURE OF EST30
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: CARBOXYLESTERASE;
COMPND   3 CHAIN: A, B, C, D, E, F, G, H, I;
COMPND   4 SYNONYM: EST30;
COMPND   5 EC: 3.1.1.1;
COMPND   6 ENGINEERED: YES;
COMPND   7 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: GEOBACILLUS STEAROTHERMOPHILUS;
SOURCE   3 ORGANISM_COMMON: BACILLUS STEAROTHERMOPHILUS;
SOURCE   4 ORGANISM_TAXID: 1422;
SOURCE   5 GENE: EST, EST30;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    CARBOXYLESTERASE, ENZYME, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    Y.FENG,Z.LUO
REVDAT   1   06-MAR-24 8ILT    0
JRNL        AUTH   Y.FENG,Z.LUO
JRNL        TITL   CRYSTAL STRUCTURE OF EST30
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.42 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.20.1-4487
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.42
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 49.54
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.9
REMARK   3   NUMBER OF REFLECTIONS             : 106615
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.217
REMARK   3   R VALUE            (WORKING SET) : 0.214
REMARK   3   FREE R VALUE                     : 0.273
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000
REMARK   3   FREE R VALUE TEST SET COUNT      : 5611
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1  2.4820 -  2.4200    0.99     7752   386  0.3710 0.3610
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : MASK
REMARK   3   SOLVENT RADIUS     : 1.20
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.290
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : NULL
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 51.69
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.42000
REMARK   3    B22 (A**2) : 4.36000
REMARK   3    B33 (A**2) : -4.20000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : -1.11000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :   NULL           NULL
REMARK   3   ANGLE     :   NULL           NULL
REMARK   3   CHIRALITY :   NULL           NULL
REMARK   3   PLANARITY :   NULL           NULL
REMARK   3   DIHEDRAL  :   NULL           NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING
REMARK   3  POSITIONS
REMARK   4
REMARK   4 8ILT COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBC ON 10-MAR-23.
REMARK 100 THE DEPOSITION ID IS D_1300036000.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 14-JAN-23
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRF
REMARK 200  BEAMLINE                       : BL19U1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.979
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS3 6M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 112238
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.420
REMARK 200  RESOLUTION RANGE LOW       (A) : 49.540
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0
REMARK 200  DATA REDUNDANCY                : 6.730
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 10.7800
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.42
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.48
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 56.76
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.84
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: ZINC ACETATE TETRAHYDRATE, PEG3350,
REMARK 280  VAPOR DIFFUSION, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       29.99400
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4, 5, 6, 7, 8, 9
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 5
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: E
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 6
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: F
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 7
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: G
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 8
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: H
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 9
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: I
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     HIS A   252
REMARK 465     HIS A   253
REMARK 465     HIS A   254
REMARK 465     HIS A   255
REMARK 465     HIS B   251
REMARK 465     HIS B   252
REMARK 465     HIS B   253
REMARK 465     HIS B   254
REMARK 465     HIS B   255
REMARK 465     HIS C   254
REMARK 465     HIS C   255
REMARK 465     GLU D   249
REMARK 465     HIS D   250
REMARK 465     HIS D   251
REMARK 465     HIS D   252
REMARK 465     HIS D   253
REMARK 465     HIS D   254
REMARK 465     HIS D   255
REMARK 465     HIS E   255
REMARK 465     GLU F   249
REMARK 465     HIS F   250
REMARK 465     HIS F   251
REMARK 465     HIS F   252
REMARK 465     HIS F   253
REMARK 465     HIS F   254
REMARK 465     HIS F   255
REMARK 465     LEU G   248
REMARK 465     GLU G   249
REMARK 465     HIS G   250
REMARK 465     HIS G   251
REMARK 465     HIS G   252
REMARK 465     HIS G   253
REMARK 465     HIS G   254
REMARK 465     HIS G   255
REMARK 465     GLU H   249
REMARK 465     HIS H   250
REMARK 465     HIS H   251
REMARK 465     HIS H   252
REMARK 465     HIS H   253
REMARK 465     HIS H   254
REMARK 465     HIS H   255
REMARK 465     GLU I   249
REMARK 465     HIS I   250
REMARK 465     HIS I   251
REMARK 465     HIS I   252
REMARK 465     HIS I   253
REMARK 465     HIS I   254
REMARK 465     HIS I   255
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    THR F    64     CG2  THR F   163              2.01
REMARK 500   N    GLY E    15     O    HOH E   301              2.01
REMARK 500   O    THR D    64     CG2  THR D   163              2.04
REMARK 500   O    ALA I   240     O    HOH I   301              2.07
REMARK 500   O    HOH A   340     O    HOH A   362              2.08
REMARK 500   OH   TYR E   239     O    HOH E   302              2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ALA A  14      -92.64   -137.54
REMARK 500    THR A  26       -9.30     73.00
REMARK 500    ASN A  28     -167.42   -161.26
REMARK 500    SER A  94     -111.10     66.36
REMARK 500    TYR A 120     -132.00   -132.54
REMARK 500    SER A 200      -38.59    -37.74
REMARK 500    ASP A 246      -76.11    -72.90
REMARK 500    PRO B   9     -179.06    -60.86
REMARK 500    ALA B  14      -42.46   -154.61
REMARK 500    SER B  94     -107.32     63.54
REMARK 500    TYR B 120     -126.79   -132.30
REMARK 500    MET B 161       55.66   -141.02
REMARK 500    MET C   2      142.48   -172.60
REMARK 500    ALA C  14     -118.94   -145.19
REMARK 500    SER C  94     -111.28     49.48
REMARK 500    THR C 106      -43.79   -137.99
REMARK 500    TYR C 120     -141.29   -137.51
REMARK 500    PRO D   9      170.41    -59.65
REMARK 500    ALA D  14      -13.04   -157.14
REMARK 500    THR D  26       -7.51     76.46
REMARK 500    SER D  94     -111.39     63.47
REMARK 500    TYR D 120     -148.97   -133.72
REMARK 500    HIS D 192       64.10   -102.18
REMARK 500    TRP D 247       16.29     59.97
REMARK 500    ALA E  14       -6.17   -153.28
REMARK 500    SER E  94     -101.83     67.52
REMARK 500    TYR E 120     -130.49   -125.77
REMARK 500    ALA F  14     -115.92   -145.66
REMARK 500    THR F  26      -11.41     69.97
REMARK 500    SER F  94     -113.16     60.79
REMARK 500    TYR F 120     -125.66   -134.51
REMARK 500    SER F 200      -37.80    -37.06
REMARK 500    ALA G  14      -68.21   -131.49
REMARK 500    THR G  26       -5.44     65.75
REMARK 500    SER G  94     -120.32     59.98
REMARK 500    TYR G 120     -129.99   -128.65
REMARK 500    GLU G 124      -73.68    -57.44
REMARK 500    PRO G 160       55.04    -92.71
REMARK 500    MET H   2      114.98   -173.30
REMARK 500    ALA H  14     -112.55   -145.80
REMARK 500    THR H  26      -10.33     79.88
REMARK 500    SER H  94     -127.10     54.11
REMARK 500    TYR H 120     -144.88   -140.01
REMARK 500    ALA H 135      -70.52    -54.28
REMARK 500    GLU H 142       37.55    -91.50
REMARK 500    MET H 161      -48.53   -135.98
REMARK 500    SER H 200      -36.93    -35.96
REMARK 500    GLN H 214      144.01   -176.72
REMARK 500    TRP H 247      -45.59     80.71
REMARK 500    MET I   2      114.47   -166.28
REMARK 500
REMARK 500 THIS ENTRY HAS      60 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
DBREF  8ILT A    2   247  UNP    Q06174   EST_GEOSE        1    246
DBREF  8ILT B    2   247  UNP    Q06174   EST_GEOSE        1    246
DBREF  8ILT C    2   247  UNP    Q06174   EST_GEOSE        1    246
DBREF  8ILT D    2   247  UNP    Q06174   EST_GEOSE        1    246
DBREF  8ILT E    2   247  UNP    Q06174   EST_GEOSE        1    246
DBREF  8ILT F    2   247  UNP    Q06174   EST_GEOSE        1    246
DBREF  8ILT G    2   247  UNP    Q06174   EST_GEOSE        1    246
DBREF  8ILT H    2   247  UNP    Q06174   EST_GEOSE        1    246
DBREF  8ILT I    2   247  UNP    Q06174   EST_GEOSE        1    246
SEQADV 8ILT MET A    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER A  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU A  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS A  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU A  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU A  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS A  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS A  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS A  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS A  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS A  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS A  255  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT MET B    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER B  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU B  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS B  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU B  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU B  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS B  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS B  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS B  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS B  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS B  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS B  255  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT MET C    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER C  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU C  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS C  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU C  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU C  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS C  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS C  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS C  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS C  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS C  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS C  255  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT MET D    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER D  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU D  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS D  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU D  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU D  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS D  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS D  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS D  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS D  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS D  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS D  255  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT MET E    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER E  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU E  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS E  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU E  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU E  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS E  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS E  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS E  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS E  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS E  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS E  255  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT MET F    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER F  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU F  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS F  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU F  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU F  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS F  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS F  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS F  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS F  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS F  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS F  255  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT MET G    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER G  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU G  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS G  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU G  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU G  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS G  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS G  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS G  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS G  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS G  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS G  255  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT MET H    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER H  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU H  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS H  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU H  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU H  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS H  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS H  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS H  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS H  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS H  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS H  255  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT MET I    1  UNP  Q06174              INITIATING METHIONINE
SEQADV 8ILT SER I  127  UNP  Q06174    MET   126 ENGINEERED MUTATION
SEQADV 8ILT LEU I  130  UNP  Q06174    GLY   129 ENGINEERED MUTATION
SEQADV 8ILT LYS I  171  UNP  Q06174    ILE   170 ENGINEERED MUTATION
SEQADV 8ILT LEU I  248  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT GLU I  249  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS I  250  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS I  251  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS I  252  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS I  253  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS I  254  UNP  Q06174              EXPRESSION TAG
SEQADV 8ILT HIS I  255  UNP  Q06174              EXPRESSION TAG
SEQRES   1 A  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 A  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 A  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 A  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 A  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 A  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 A  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 A  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 A  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 A  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 A  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 A  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 A  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 A  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 A  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 A  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 A  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 A  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 A  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 A  255  LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 B  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 B  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 B  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 B  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 B  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 B  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 B  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 B  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 B  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 B  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 B  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 B  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 B  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 B  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 B  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 B  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 B  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 B  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 B  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 B  255  LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 C  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 C  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 C  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 C  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 C  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 C  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 C  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 C  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 C  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 C  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 C  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 C  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 C  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 C  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 C  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 C  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 C  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 C  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 C  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 C  255  LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 D  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 D  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 D  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 D  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 D  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 D  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 D  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 D  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 D  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 D  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 D  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 D  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 D  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 D  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 D  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 D  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 D  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 D  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 D  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 D  255  LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 E  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 E  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 E  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 E  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 E  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 E  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 E  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 E  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 E  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 E  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 E  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 E  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 E  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 E  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 E  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 E  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 E  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 E  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 E  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 E  255  LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 F  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 F  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 F  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 F  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 F  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 F  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 F  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 F  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 F  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 F  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 F  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 F  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 F  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 F  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 F  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 F  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 F  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 F  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 F  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 F  255  LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 G  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 G  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 G  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 G  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 G  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 G  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 G  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 G  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 G  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 G  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 G  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 G  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 G  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 G  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 G  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 G  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 G  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 G  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 G  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 G  255  LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 H  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 H  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 H  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 H  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 H  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 H  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 H  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 H  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 H  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 H  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 H  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 H  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 H  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 H  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 H  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 H  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 H  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 H  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 H  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 H  255  LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 I  255  MET MET LYS ILE VAL PRO PRO LYS PRO PHE PHE PHE GLU
SEQRES   2 I  255  ALA GLY GLU ARG ALA VAL LEU LEU LEU HIS GLY PHE THR
SEQRES   3 I  255  GLY ASN SER ALA ASP VAL ARG MET LEU GLY ARG PHE LEU
SEQRES   4 I  255  GLU SER LYS GLY TYR THR CYS HIS ALA PRO ILE TYR LYS
SEQRES   5 I  255  GLY HIS GLY VAL PRO PRO GLU GLU LEU VAL HIS THR GLY
SEQRES   6 I  255  PRO ASP ASP TRP TRP GLN ASP VAL MET ASN GLY TYR GLU
SEQRES   7 I  255  PHE LEU LYS ASN LYS GLY TYR GLU LYS ILE ALA VAL ALA
SEQRES   8 I  255  GLY LEU SER LEU GLY GLY VAL PHE SER LEU LYS LEU GLY
SEQRES   9 I  255  TYR THR VAL PRO ILE GLU GLY ILE VAL THR MET CYS ALA
SEQRES  10 I  255  PRO MET TYR ILE LYS SER GLU GLU THR SER TYR GLU LEU
SEQRES  11 I  255  VAL LEU GLU TYR ALA ARG GLU TYR LYS LYS ARG GLU GLY
SEQRES  12 I  255  LYS SER GLU GLU GLN ILE GLU GLN GLU MET GLU LYS PHE
SEQRES  13 I  255  LYS GLN THR PRO MET LYS THR LEU LYS ALA LEU GLN GLU
SEQRES  14 I  255  LEU LYS ALA ASP VAL ARG ASP HIS LEU ASP LEU ILE TYR
SEQRES  15 I  255  ALA PRO THR PHE VAL VAL GLN ALA ARG HIS ASP GLU MET
SEQRES  16 I  255  ILE ASN PRO ASP SER ALA ASN ILE ILE TYR ASN GLU ILE
SEQRES  17 I  255  GLU SER PRO VAL LYS GLN ILE LYS TRP TYR GLU GLN SER
SEQRES  18 I  255  GLY HIS VAL ILE THR LEU ASP GLN GLU LYS ASP GLN LEU
SEQRES  19 I  255  HIS GLU ASP ILE TYR ALA PHE LEU GLU SER LEU ASP TRP
SEQRES  20 I  255  LEU GLU HIS HIS HIS HIS HIS HIS
FORMUL  10  HOH   *415(H2 O)
HELIX    1 AA1 ASN A   28  ASP A   31  5                                   4
HELIX    2 AA2 VAL A   32  LYS A   42  1                                  11
HELIX    3 AA3 PRO A   57  VAL A   62  1                                   6
HELIX    4 AA4 GLY A   65  LYS A   83  1                                  19
HELIX    5 AA5 SER A   94  TYR A  105  1                                  12
HELIX    6 AA6 ILE A  121  GLU A  142  1                                  22
HELIX    7 AA7 SER A  145  GLN A  158  1                                  14
HELIX    8 AA8 LYS A  162  HIS A  177  1                                  16
HELIX    9 AA9 LEU A  178  ILE A  181  5                                   4
HELIX   10 AB1 ASP A  199  ILE A  208  1                                  10
HELIX   11 AB2 VAL A  224  ASP A  228  5                                   5
HELIX   12 AB3 GLU A  230  SER A  244  1                                  15
HELIX   13 AB4 SER B   29  LYS B   42  1                                  14
HELIX   14 AB5 PRO B   57  VAL B   62  1                                   6
HELIX   15 AB6 GLY B   65  LYS B   83  1                                  19
HELIX   16 AB7 SER B   94  TYR B  105  1                                  12
HELIX   17 AB8 ILE B  121  GLU B  142  1                                  22
HELIX   18 AB9 SER B  145  GLN B  158  1                                  14
HELIX   19 AC1 MET B  161  HIS B  177  1                                  17
HELIX   20 AC2 LEU B  178  ILE B  181  5                                   4
HELIX   21 AC3 ASP B  199  ILE B  208  1                                  10
HELIX   22 AC4 VAL B  224  ASP B  228  5                                   5
HELIX   23 AC5 GLU B  230  SER B  244  1                                  15
HELIX   24 AC6 ASN C   28  ASP C   31  5                                   4
HELIX   25 AC7 VAL C   32  LYS C   42  1                                  11
HELIX   26 AC8 PRO C   57  VAL C   62  1                                   6
HELIX   27 AC9 GLY C   65  LYS C   83  1                                  19
HELIX   28 AD1 SER C   94  TYR C  105  1                                  12
HELIX   29 AD2 ILE C  121  GLU C  142  1                                  22
HELIX   30 AD3 SER C  145  LYS C  157  1                                  13
HELIX   31 AD4 PRO C  160  ASP C  176  1                                  17
HELIX   32 AD5 HIS C  177  ILE C  181  5                                   5
HELIX   33 AD6 ASP C  199  ILE C  208  1                                  10
HELIX   34 AD7 VAL C  224  ASP C  228  5                                   5
HELIX   35 AD8 GLU C  230  SER C  244  1                                  15
HELIX   36 AD9 ASN D   28  ASP D   31  5                                   4
HELIX   37 AE1 VAL D   32  LYS D   42  1                                  11
HELIX   38 AE2 PRO D   57  VAL D   62  1                                   6
HELIX   39 AE3 GLY D   65  LYS D   83  1                                  19
HELIX   40 AE4 SER D   94  TYR D  105  1                                  12
HELIX   41 AE5 ILE D  121  GLU D  142  1                                  22
HELIX   42 AE6 SER D  145  LYS D  157  1                                  13
HELIX   43 AE7 PRO D  160  ASP D  176  1                                  17
HELIX   44 AE8 HIS D  177  ILE D  181  5                                   5
HELIX   45 AE9 ASP D  199  ILE D  208  1                                  10
HELIX   46 AF1 VAL D  224  ASP D  228  5                                   5
HELIX   47 AF2 GLU D  230  SER D  244  1                                  15
HELIX   48 AF3 ASN E   28  ASP E   31  5                                   4
HELIX   49 AF4 VAL E   32  LYS E   42  1                                  11
HELIX   50 AF5 PRO E   57  VAL E   62  1                                   6
HELIX   51 AF6 GLY E   65  LYS E   83  1                                  19
HELIX   52 AF7 SER E   94  TYR E  105  1                                  12
HELIX   53 AF8 ILE E  121  GLU E  142  1                                  22
HELIX   54 AF9 SER E  145  LYS E  157  1                                  13
HELIX   55 AG1 PRO E  160  LEU E  178  1                                  19
HELIX   56 AG2 ASP E  179  ILE E  181  5                                   3
HELIX   57 AG3 ASP E  199  ILE E  208  1                                  10
HELIX   58 AG4 VAL E  224  ASP E  228  5                                   5
HELIX   59 AG5 GLU E  230  LEU E  245  1                                  16
HELIX   60 AG6 ASN F   28  ASP F   31  5                                   4
HELIX   61 AG7 VAL F   32  SER F   41  1                                  10
HELIX   62 AG8 PRO F   57  VAL F   62  1                                   6
HELIX   63 AG9 GLY F   65  ASN F   82  1                                  18
HELIX   64 AH1 SER F   94  GLY F  104  1                                  11
HELIX   65 AH2 ILE F  121  GLY F  143  1                                  23
HELIX   66 AH3 SER F  145  LYS F  157  1                                  13
HELIX   67 AH4 PRO F  160  LEU F  178  1                                  19
HELIX   68 AH5 ASP F  179  ILE F  181  5                                   3
HELIX   69 AH6 ASP F  199  ILE F  208  1                                  10
HELIX   70 AH7 VAL F  224  ASP F  228  5                                   5
HELIX   71 AH8 GLU F  230  SER F  244  1                                  15
HELIX   72 AH9 ASN G   28  ASP G   31  5                                   4
HELIX   73 AI1 VAL G   32  LYS G   42  1                                  11
HELIX   74 AI2 PRO G   57  VAL G   62  1                                   6
HELIX   75 AI3 GLY G   65  LYS G   83  1                                  19
HELIX   76 AI4 SER G   94  TYR G  105  1                                  12
HELIX   77 AI5 ILE G  121  GLU G  142  1                                  22
HELIX   78 AI6 SER G  145  LYS G  157  1                                  13
HELIX   79 AI7 MET G  161  HIS G  177  1                                  17
HELIX   80 AI8 LEU G  178  ILE G  181  5                                   4
HELIX   81 AI9 ASP G  199  ILE G  208  1                                  10
HELIX   82 AJ1 GLU G  230  SER G  244  1                                  15
HELIX   83 AJ2 ASN H   28  ASP H   31  5                                   4
HELIX   84 AJ3 VAL H   32  LYS H   42  1                                  11
HELIX   85 AJ4 PRO H   57  VAL H   62  1                                   6
HELIX   86 AJ5 GLY H   65  LYS H   83  1                                  19
HELIX   87 AJ6 SER H   94  LEU H  103  1                                  10
HELIX   88 AJ7 GLY H  104  THR H  106  5                                   3
HELIX   89 AJ8 ILE H  121  GLU H  142  1                                  22
HELIX   90 AJ9 SER H  145  LYS H  157  1                                  13
HELIX   91 AK1 MET H  161  ASP H  176  1                                  16
HELIX   92 AK2 HIS H  177  ILE H  181  5                                   5
HELIX   93 AK3 ASP H  199  ILE H  208  1                                  10
HELIX   94 AK4 GLU H  230  LEU H  245  1                                  16
HELIX   95 AK5 SER I   29  SER I   41  1                                  13
HELIX   96 AK6 PRO I   57  VAL I   62  1                                   6
HELIX   97 AK7 GLY I   65  LYS I   83  1                                  19
HELIX   98 AK8 SER I   94  GLY I  104  1                                  11
HELIX   99 AK9 ILE I  121  GLU I  142  1                                  22
HELIX  100 AL1 SER I  145  LYS I  157  1                                  13
HELIX  101 AL2 THR I  163  HIS I  177  1                                  15
HELIX  102 AL3 LEU I  178  ILE I  181  5                                   4
HELIX  103 AL4 ASP I  199  ILE I  208  1                                  10
HELIX  104 AL5 VAL I  224  ASP I  228  5                                   5
HELIX  105 AL6 GLU I  230  LEU I  245  1                                  16
SHEET    1 AA1 7 PHE A  10  PHE A  12  0
SHEET    2 AA1 7 THR A  45  ALA A  48 -1  O  CYS A  46   N  PHE A  12
SHEET    3 AA1 7 ALA A  18  LEU A  22  1  N  LEU A  21   O  HIS A  47
SHEET    4 AA1 7 ILE A  88  LEU A  93  1  O  LEU A  93   N  LEU A  22
SHEET    5 AA1 7 ILE A 112  MET A 115  1  O  VAL A 113   N  GLY A  92
SHEET    6 AA1 7 THR A 185  ALA A 190  1  O  VAL A 188   N  THR A 114
SHEET    7 AA1 7 LYS A 213  TYR A 218  1  O  GLN A 214   N  VAL A 187
SHEET    1 AA2 7 PHE B  10  PHE B  12  0
SHEET    2 AA2 7 THR B  45  ALA B  48 -1  O  CYS B  46   N  PHE B  12
SHEET    3 AA2 7 ALA B  18  LEU B  22  1  N  LEU B  21   O  HIS B  47
SHEET    4 AA2 7 ILE B  88  LEU B  93  1  O  ALA B  89   N  LEU B  20
SHEET    5 AA2 7 ILE B 112  MET B 115  1  O  MET B 115   N  GLY B  92
SHEET    6 AA2 7 THR B 185  ALA B 190  1  O  PHE B 186   N  ILE B 112
SHEET    7 AA2 7 LYS B 213  TYR B 218  1  O  GLN B 214   N  VAL B 187
SHEET    1 AA3 7 PHE C  10  PHE C  12  0
SHEET    2 AA3 7 THR C  45  ALA C  48 -1  O  CYS C  46   N  PHE C  12
SHEET    3 AA3 7 ALA C  18  LEU C  22  1  N  LEU C  21   O  HIS C  47
SHEET    4 AA3 7 ILE C  88  LEU C  93  1  O  ALA C  91   N  LEU C  20
SHEET    5 AA3 7 ILE C 112  MET C 115  1  O  MET C 115   N  GLY C  92
SHEET    6 AA3 7 THR C 185  ALA C 190  1  O  VAL C 188   N  THR C 114
SHEET    7 AA3 7 LYS C 213  TYR C 218  1  O  GLN C 214   N  VAL C 187
SHEET    1 AA4 7 PHE D  10  PHE D  12  0
SHEET    2 AA4 7 THR D  45  ALA D  48 -1  O  CYS D  46   N  PHE D  12
SHEET    3 AA4 7 ALA D  18  LEU D  22  1  N  LEU D  21   O  HIS D  47
SHEET    4 AA4 7 ILE D  88  LEU D  93  1  O  ALA D  91   N  LEU D  20
SHEET    5 AA4 7 ILE D 112  MET D 115  1  O  MET D 115   N  GLY D  92
SHEET    6 AA4 7 THR D 185  ALA D 190  1  O  PHE D 186   N  ILE D 112
SHEET    7 AA4 7 LYS D 213  TYR D 218  1  O  GLN D 214   N  VAL D 187
SHEET    1 AA5 7 PHE E  10  PHE E  12  0
SHEET    2 AA5 7 THR E  45  ALA E  48 -1  O  CYS E  46   N  PHE E  12
SHEET    3 AA5 7 ALA E  18  LEU E  22  1  N  LEU E  21   O  HIS E  47
SHEET    4 AA5 7 ILE E  88  LEU E  93  1  O  ALA E  91   N  LEU E  20
SHEET    5 AA5 7 ILE E 112  MET E 115  1  O  VAL E 113   N  GLY E  92
SHEET    6 AA5 7 THR E 185  ALA E 190  1  O  PHE E 186   N  ILE E 112
SHEET    7 AA5 7 LYS E 213  TYR E 218  1  O  GLN E 214   N  VAL E 187
SHEET    1 AA6 7 PHE F  10  PHE F  12  0
SHEET    2 AA6 7 THR F  45  ALA F  48 -1  O  CYS F  46   N  PHE F  12
SHEET    3 AA6 7 ALA F  18  LEU F  22  1  N  LEU F  21   O  HIS F  47
SHEET    4 AA6 7 ILE F  88  LEU F  93  1  O  ALA F  89   N  ALA F  18
SHEET    5 AA6 7 ILE F 112  MET F 115  1  O  MET F 115   N  GLY F  92
SHEET    6 AA6 7 THR F 185  ALA F 190  1  O  PHE F 186   N  ILE F 112
SHEET    7 AA6 7 LYS F 213  TYR F 218  1  O  GLN F 214   N  VAL F 187
SHEET    1 AA7 7 PHE G  10  PHE G  12  0
SHEET    2 AA7 7 THR G  45  ALA G  48 -1  O  CYS G  46   N  PHE G  12
SHEET    3 AA7 7 ALA G  18  LEU G  22  1  N  LEU G  21   O  HIS G  47
SHEET    4 AA7 7 ILE G  88  LEU G  93  1  O  ALA G  91   N  LEU G  22
SHEET    5 AA7 7 ILE G 112  MET G 115  1  O  MET G 115   N  GLY G  92
SHEET    6 AA7 7 THR G 185  ALA G 190  1  O  PHE G 186   N  THR G 114
SHEET    7 AA7 7 LYS G 213  TYR G 218  1  O  TYR G 218   N  GLN G 189
SHEET    1 AA8 2 PHE H  10  PHE H  12  0
SHEET    2 AA8 2 CYS H  46  ALA H  48 -1  O  CYS H  46   N  PHE H  12
SHEET    1 AA9 5 ALA H  18  LEU H  22  0
SHEET    2 AA9 5 ILE H  88  LEU H  93  1  O  ALA H  89   N  LEU H  20
SHEET    3 AA9 5 ILE H 112  MET H 115  1  O  MET H 115   N  GLY H  92
SHEET    4 AA9 5 THR H 185  ALA H 190  1  O  VAL H 188   N  THR H 114
SHEET    5 AA9 5 ILE H 215  TYR H 218  1  O  LYS H 216   N  VAL H 187
SHEET    1 AB1 2 PHE I  10  PHE I  12  0
SHEET    2 AB1 2 CYS I  46  ALA I  48 -1  O  CYS I  46   N  PHE I  12
SHEET    1 AB2 5 ALA I  18  LEU I  22  0
SHEET    2 AB2 5 ILE I  88  LEU I  93  1  O  ALA I  91   N  LEU I  20
SHEET    3 AB2 5 ILE I 112  MET I 115  1  O  MET I 115   N  GLY I  92
SHEET    4 AB2 5 THR I 185  ALA I 190  1  O  PHE I 186   N  ILE I 112
SHEET    5 AB2 5 LYS I 213  TYR I 218  1  O  TYR I 218   N  GLN I 189
CRYST1  149.964   59.988  166.039  90.00  99.24  90.00 P 1 21 1     18
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.006668  0.000000  0.001085        0.00000
SCALE2      0.000000  0.016670  0.000000        0.00000
SCALE3      0.000000  0.000000  0.006102        0.00000
TER    2044      HIS A 251
TER    4085      HIS B 250
TER    6144      HIS C 253
TER    8154      LEU D 248
TER   10231      HIS E 254
TER   12241      LEU F 248
TER   14246      TRP G 247
TER   16256      LEU H 248
TER   18266      LEU I 248
MASTER      375    0    0  105   63    0    0    618644    9    0  180
END