longtext: 8vce-pdb

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HEADER    HYDROLASE                               14-DEC-23   8VCE
TITLE     CRYSTAL STRUCTURE OF PLANT CARBOXYLESTERASE 20
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: PROBABLE CARBOXYLESTERASE 120;
COMPND   3 CHAIN: A, B;
COMPND   4 SYNONYM: ATCXE20;
COMPND   5 EC: 3.1.1.1;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: ARABIDOPSIS THALIANA;
SOURCE   3 ORGANISM_COMMON: THALE CRESS;
SOURCE   4 ORGANISM_TAXID: 3702;
SOURCE   5 GENE: CXE20, AT5G62180, MMI9.26;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    CARBOXYLESTERASE 20, CXE20, STRIGOLACTONE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    M.PALAYAM,N.SHABEK
REVDAT   1   07-AUG-24 8VCE    0
JRNL        AUTH   M.PALAYAM
JRNL        TITL   STRUCTURAL INSIGHTS INTO STRIGOLACTONE CATABOLISM BY
JRNL        TITL 2 CARBOXYLESTERASES REVEAL A CONSERVED ALLOSTERIC REGULATION
JRNL        REF    NAT COMMUN                                 2024
JRNL        REFN                   ESSN 2041-1723
JRNL        DOI    10.1038/S41467-024-50928-3
REMARK   2
REMARK   2 RESOLUTION.    1.85 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.19.2_4158
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.85
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 48.00
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.7
REMARK   3   NUMBER OF REFLECTIONS             : 55318
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.170
REMARK   3   R VALUE            (WORKING SET) : 0.169
REMARK   3   FREE R VALUE                     : 0.197
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 3.630
REMARK   3   FREE R VALUE TEST SET COUNT      : 2007
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 48.0000 -  4.4600    1.00     4042   154  0.1586 0.1586
REMARK   3     2  4.4600 -  3.5400    1.00     3896   149  0.1464 0.1533
REMARK   3     3  3.5400 -  3.0900    1.00     3858   143  0.1654 0.2246
REMARK   3     4  3.0900 -  2.8100    1.00     3819   150  0.1734 0.2006
REMARK   3     5  2.8100 -  2.6100    1.00     3820   143  0.1696 0.2056
REMARK   3     6  2.6100 -  2.4500    1.00     3812   142  0.1650 0.1877
REMARK   3     7  2.4500 -  2.3300    1.00     3789   139  0.1573 0.1810
REMARK   3     8  2.3300 -  2.2300    1.00     3776   138  0.1917 0.2254
REMARK   3     9  2.2300 -  2.1400    1.00     3817   144  0.1737 0.2227
REMARK   3    10  2.1400 -  2.0700    1.00     3752   149  0.1751 0.2405
REMARK   3    11  2.0700 -  2.0000    1.00     3751   133  0.1805 0.2173
REMARK   3    12  2.0000 -  1.9500    1.00     3796   134  0.1923 0.2727
REMARK   3    13  1.9500 -  1.9000    1.00     3762   154  0.2376 0.2922
REMARK   3    14  1.9000 -  1.8500    0.96     3621   135  0.2358 0.2739
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.200
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 19.000
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.008           5096
REMARK   3   ANGLE     :  1.027           6936
REMARK   3   CHIRALITY :  0.060            770
REMARK   3   PLANARITY :  0.009            902
REMARK   3   DIHEDRAL  :  5.641            698
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 8VCE COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 15-DEC-23.
REMARK 100 THE DEPOSITION ID IS D_1000279918.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 02-APR-23
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : ALS
REMARK 200  BEAMLINE                       : 8.2.2
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.0
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 55388
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.850
REMARK 200  RESOLUTION RANGE LOW       (A) : 48.000
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.0
REMARK 200  DATA REDUNDANCY                : 12.60
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : 0.03500
REMARK 200   FOR THE DATA SET  : 23.2000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.85
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.90
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : 0.23000
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER 1.19.2_4158
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 44.24
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.21
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 10% W/V PEG8000, 20% V/V ETHYLENE
REMARK 280  GLYCOL, 0.1M IMIDAZOLE, 0.03M SODIUM NITRATE, 0.03M DISODIUM
REMARK 280  HYDROGEN PHOSPHATE AND 0.03M AMMONIUM SULFATE, VAPOR DIFFUSION,
REMARK 280  HANGING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       33.29350
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       50.15650
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       47.85050
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       50.15650
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       33.29350
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       47.85050
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     SER A     2
REMARK 465     GLU A     3
REMARK 465     PRO A     4
REMARK 465     SER A     5
REMARK 465     ASN A    71
REMARK 465     GLU A    72
REMARK 465     GLY A    73
REMARK 465     ASN A    74
REMARK 465     VAL A    75
REMARK 465     SER A    76
REMARK 465     SER A    77
REMARK 465     LEU A   327
REMARK 465     MET B     1
REMARK 465     SER B     2
REMARK 465     GLU B     3
REMARK 465     PRO B     4
REMARK 465     ASP B    21
REMARK 465     GLY B    22
REMARK 465     ASN B    71
REMARK 465     GLU B    72
REMARK 465     GLY B    73
REMARK 465     ASN B    74
REMARK 465     VAL B    75
REMARK 465     SER B    76
REMARK 465     SER B    77
REMARK 465     LEU B   327
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASP A  21       14.73   -159.33
REMARK 500    CYS A  94      152.74     79.61
REMARK 500    SER A 166     -121.30     57.75
REMARK 500    ASP A 237     -169.83   -110.58
REMARK 500    CYS B  94      151.80     76.77
REMARK 500    SER B 166     -117.81     56.93
REMARK 500    CYS B 219       64.09   -150.02
REMARK 500    CYS B 219       67.85   -151.81
REMARK 500    ASP B 239       45.53    -86.61
REMARK 500
REMARK 500 REMARK: NULL
DBREF  8VCE A    1   327  UNP    Q9LVB8   CXE20_ARATH      1    327
DBREF  8VCE B    1   327  UNP    Q9LVB8   CXE20_ARATH      1    327
SEQRES   1 A  327  MET SER GLU PRO SER PRO ILE ALA ASP PRO TYR ALA TYR
SEQRES   2 A  327  LEU ASN ILE VAL ASN ASN PRO ASP GLY SER ILE THR ARG
SEQRES   3 A  327  ASP LEU SER ASN PHE PRO CYS THR ALA ALA THR PRO ASP
SEQRES   4 A  327  PRO SER PRO LEU ASN PRO ALA VAL SER LYS ASP LEU PRO
SEQRES   5 A  327  VAL ASN GLN LEU LYS SER THR TRP LEU ARG LEU TYR LEU
SEQRES   6 A  327  PRO SER SER ALA VAL ASN GLU GLY ASN VAL SER SER GLN
SEQRES   7 A  327  LYS LEU PRO ILE VAL VAL TYR TYR HIS GLY GLY GLY PHE
SEQRES   8 A  327  ILE LEU CYS SER VAL ASP MET GLN LEU PHE HIS ASP PHE
SEQRES   9 A  327  CYS SER GLU VAL ALA ARG ASP LEU ASN ALA ILE VAL VAL
SEQRES  10 A  327  SER PRO SER TYR ARG LEU ALA PRO GLU HIS ARG LEU PRO
SEQRES  11 A  327  ALA ALA TYR ASP ASP GLY VAL GLU ALA LEU ASP TRP ILE
SEQRES  12 A  327  LYS THR SER ASP ASP GLU TRP ILE LYS SER HIS ALA ASP
SEQRES  13 A  327  PHE SER ASN VAL PHE LEU MET GLY THR SER ALA GLY GLY
SEQRES  14 A  327  ASN LEU ALA TYR ASN VAL GLY LEU ARG SER VAL ASP SER
SEQRES  15 A  327  VAL SER ASP LEU SER PRO LEU GLN ILE ARG GLY LEU ILE
SEQRES  16 A  327  LEU HIS HIS PRO PHE PHE GLY GLY GLU GLU ARG SER GLU
SEQRES  17 A  327  SER GLU ILE ARG LEU MET ASN ASP GLN VAL CYS PRO PRO
SEQRES  18 A  327  ILE VAL THR ASP VAL MET TRP ASP LEU SER LEU PRO VAL
SEQRES  19 A  327  GLY VAL ASP ARG ASP HIS GLU TYR SER ASN PRO THR VAL
SEQRES  20 A  327  GLY ASP GLY SER GLU LYS LEU GLU LYS ILE GLY ARG LEU
SEQRES  21 A  327  ARG TRP LYS VAL MET MET ILE GLY GLY GLU ASP ASP PRO
SEQRES  22 A  327  MET ILE ASP LEU GLN LYS ASP VAL ALA LYS LEU MET LYS
SEQRES  23 A  327  LYS LYS GLY VAL GLU VAL VAL GLU HIS TYR THR GLY GLY
SEQRES  24 A  327  HIS VAL HIS GLY ALA GLU ILE ARG ASP PRO SER LYS ARG
SEQRES  25 A  327  LYS THR LEU PHE LEU SER ILE LYS ASN PHE ILE PHE SER
SEQRES  26 A  327  VAL LEU
SEQRES   1 B  327  MET SER GLU PRO SER PRO ILE ALA ASP PRO TYR ALA TYR
SEQRES   2 B  327  LEU ASN ILE VAL ASN ASN PRO ASP GLY SER ILE THR ARG
SEQRES   3 B  327  ASP LEU SER ASN PHE PRO CYS THR ALA ALA THR PRO ASP
SEQRES   4 B  327  PRO SER PRO LEU ASN PRO ALA VAL SER LYS ASP LEU PRO
SEQRES   5 B  327  VAL ASN GLN LEU LYS SER THR TRP LEU ARG LEU TYR LEU
SEQRES   6 B  327  PRO SER SER ALA VAL ASN GLU GLY ASN VAL SER SER GLN
SEQRES   7 B  327  LYS LEU PRO ILE VAL VAL TYR TYR HIS GLY GLY GLY PHE
SEQRES   8 B  327  ILE LEU CYS SER VAL ASP MET GLN LEU PHE HIS ASP PHE
SEQRES   9 B  327  CYS SER GLU VAL ALA ARG ASP LEU ASN ALA ILE VAL VAL
SEQRES  10 B  327  SER PRO SER TYR ARG LEU ALA PRO GLU HIS ARG LEU PRO
SEQRES  11 B  327  ALA ALA TYR ASP ASP GLY VAL GLU ALA LEU ASP TRP ILE
SEQRES  12 B  327  LYS THR SER ASP ASP GLU TRP ILE LYS SER HIS ALA ASP
SEQRES  13 B  327  PHE SER ASN VAL PHE LEU MET GLY THR SER ALA GLY GLY
SEQRES  14 B  327  ASN LEU ALA TYR ASN VAL GLY LEU ARG SER VAL ASP SER
SEQRES  15 B  327  VAL SER ASP LEU SER PRO LEU GLN ILE ARG GLY LEU ILE
SEQRES  16 B  327  LEU HIS HIS PRO PHE PHE GLY GLY GLU GLU ARG SER GLU
SEQRES  17 B  327  SER GLU ILE ARG LEU MET ASN ASP GLN VAL CYS PRO PRO
SEQRES  18 B  327  ILE VAL THR ASP VAL MET TRP ASP LEU SER LEU PRO VAL
SEQRES  19 B  327  GLY VAL ASP ARG ASP HIS GLU TYR SER ASN PRO THR VAL
SEQRES  20 B  327  GLY ASP GLY SER GLU LYS LEU GLU LYS ILE GLY ARG LEU
SEQRES  21 B  327  ARG TRP LYS VAL MET MET ILE GLY GLY GLU ASP ASP PRO
SEQRES  22 B  327  MET ILE ASP LEU GLN LYS ASP VAL ALA LYS LEU MET LYS
SEQRES  23 B  327  LYS LYS GLY VAL GLU VAL VAL GLU HIS TYR THR GLY GLY
SEQRES  24 B  327  HIS VAL HIS GLY ALA GLU ILE ARG ASP PRO SER LYS ARG
SEQRES  25 B  327  LYS THR LEU PHE LEU SER ILE LYS ASN PHE ILE PHE SER
SEQRES  26 B  327  VAL LEU
HET    IMD  A 401       5
HET    EDO  A 402       4
HET    IMD  B 401       5
HET    EDO  B 402       4
HETNAM     IMD IMIDAZOLE
HETNAM     EDO 1,2-ETHANEDIOL
HETSYN     EDO ETHYLENE GLYCOL
FORMUL   3  IMD    2(C3 H5 N2 1+)
FORMUL   4  EDO    2(C2 H6 O2)
FORMUL   7  HOH   *390(H2 O)
HELIX    1 AA1 ASP A    9  ASN A   15  1                                   7
HELIX    2 AA2 LEU A   28  PHE A   31  5                                   4
HELIX    3 AA3 SER A   68  VAL A   70  5                                   3
HELIX    4 AA4 MET A   98  ASN A  113  1                                  16
HELIX    5 AA5 PRO A  130  SER A  146  1                                  17
HELIX    6 AA6 ASP A  148  HIS A  154  1                                   7
HELIX    7 AA7 SER A  166  ASP A  181  1                                  16
HELIX    8 AA8 SER A  182  SER A  187  1                                   6
HELIX    9 AA9 SER A  207  LEU A  213  1                                   7
HELIX   10 AB1 PRO A  220  LEU A  232  1                                  13
HELIX   11 AB2 LYS A  253  LEU A  260  1                                   8
HELIX   12 AB3 MET A  274  LYS A  288  1                                  15
HELIX   13 AB4 GLY A  303  ASP A  308  1                                   6
HELIX   14 AB5 LYS A  311  SER A  325  1                                  15
HELIX   15 AB6 ASP B    9  ASN B   15  1                                   7
HELIX   16 AB7 SER B   68  VAL B   70  5                                   3
HELIX   17 AB8 MET B   98  ASN B  113  1                                  16
HELIX   18 AB9 PRO B  130  THR B  145  1                                  16
HELIX   19 AC1 ASP B  148  HIS B  154  1                                   7
HELIX   20 AC2 SER B  166  VAL B  180  1                                  15
HELIX   21 AC3 SER B  182  SER B  187  1                                   6
HELIX   22 AC4 SER B  207  LEU B  213  1                                   7
HELIX   23 AC5 PRO B  220  LEU B  232  1                                  13
HELIX   24 AC6 LYS B  253  LEU B  260  1                                   8
HELIX   25 AC7 MET B  274  LYS B  288  1                                  15
HELIX   26 AC8 GLY B  303  ASP B  308  1                                   6
HELIX   27 AC9 LYS B  311  SER B  325  1                                  15
SHEET    1 AA1 2 ILE A  16  ASN A  18  0
SHEET    2 AA1 2 ILE A  24  ARG A  26 -1  O  THR A  25   N  VAL A  17
SHEET    1 AA2 8 ALA A  46  ASN A  54  0
SHEET    2 AA2 8 THR A  59  PRO A  66 -1  O  THR A  59   N  VAL A  53
SHEET    3 AA2 8 ILE A 115  PRO A 119 -1  O  SER A 118   N  ARG A  62
SHEET    4 AA2 8 LEU A  80  TYR A  86  1  N  TYR A  85   O  VAL A 117
SHEET    5 AA2 8 ALA A 155  THR A 165  1  O  MET A 163   N  VAL A  84
SHEET    6 AA2 8 ILE A 191  HIS A 197  1  O  ILE A 195   N  LEU A 162
SHEET    7 AA2 8 LYS A 263  GLY A 269  1  O  MET A 265   N  LEU A 196
SHEET    8 AA2 8 VAL A 292  THR A 297  1  O  VAL A 293   N  VAL A 264
SHEET    1 AA3 2 ILE B  16  ASN B  18  0
SHEET    2 AA3 2 ILE B  24  ARG B  26 -1  O  THR B  25   N  VAL B  17
SHEET    1 AA4 8 ALA B  46  ASN B  54  0
SHEET    2 AA4 8 THR B  59  PRO B  66 -1  O  LEU B  63   N  LYS B  49
SHEET    3 AA4 8 ILE B 115  PRO B 119 -1  O  SER B 118   N  ARG B  62
SHEET    4 AA4 8 LEU B  80  TYR B  86  1  N  TYR B  85   O  VAL B 117
SHEET    5 AA4 8 ALA B 155  THR B 165  1  O  MET B 163   N  VAL B  84
SHEET    6 AA4 8 ILE B 191  HIS B 197  1  O  ARG B 192   N  VAL B 160
SHEET    7 AA4 8 LYS B 263  GLY B 269  1  O  MET B 265   N  LEU B 196
SHEET    8 AA4 8 GLU B 291  THR B 297  1  O  VAL B 293   N  VAL B 264
CISPEP   1 ALA A  124    PRO A  125          0         3.08
CISPEP   2 LEU A  129    PRO A  130          0         9.21
CISPEP   3 SER A  187    PRO A  188          0         8.91
CISPEP   4 ALA B  124    PRO B  125          0         3.71
CISPEP   5 LEU B  129    PRO B  130          0         7.49
CISPEP   6 SER B  187    PRO B  188          0         9.33
CRYST1   66.587   95.701  100.313  90.00  90.00  90.00 P 21 21 21    8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.015018  0.000000  0.000000        0.00000
SCALE2      0.000000  0.010449  0.000000        0.00000
SCALE3      0.000000  0.000000  0.009969        0.00000
TER    2477      VAL A 326
TER    4952      VAL B 326
MASTER      269    0    4   27   20    0    0    6 5308    2   18   52
END