longtext: 8zrg-pdb

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HEADER    HYDROLASE                               04-JUN-24   8ZRG
TITLE     ARABIDOPSIS CARBOXYLESTERASE CXE15 C14S MUTANT
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: STRIGOLACTONES HYDROLASE CXE15;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: CARBOXYLESTERASE 15,ATCXE15;
COMPND   5 EC: 3.1.1.-;
COMPND   6 ENGINEERED: YES;
COMPND   7 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: ARABIDOPSIS THALIANA;
SOURCE   3 ORGANISM_COMMON: THALE CRESS;
SOURCE   4 ORGANISM_TAXID: 3702;
SOURCE   5 GENE: CXE15, AT5G06570, F15M7.10;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI 'BL21-GOLD(DE3)PLYSS AG';
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 866768
KEYWDS    CATABOLIC ENZYME, STRIGOLACTOONE, CARBOXYLESTERASE, CXE15, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    S.T.AROLD,U.F.S.HAMEED
REVDAT   1   28-MAY-25 8ZRG    0
JRNL        AUTH   S.T.AROLD,U.F.S.HAMEED
JRNL        TITL   ARABIDOPSIS CARBOXYLESTERASE CXE15 C14S MUTANT
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.75 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC V5.7
REMARK   3   AUTHORS     : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK   3               : NICHOLLS,WINN,LONG,VAGIN
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.75
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 49.07
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.7
REMARK   3   NUMBER OF REFLECTIONS             : 10510
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.181
REMARK   3   R VALUE            (WORKING SET) : 0.177
REMARK   3   FREE R VALUE                     : 0.225
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 8.500
REMARK   3   FREE R VALUE TEST SET COUNT      : 972
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 2491
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 0
REMARK   3   SOLVENT ATOMS            : 29
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 64.47
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : -0.17000
REMARK   3    B22 (A**2) : -0.17000
REMARK   3    B33 (A**2) : 0.33000
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 1.200
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.315
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.240
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 13.015
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.
REMARK   3   DISTANCE RESTRAINTS.                    RMS    SIGMA
REMARK   3    BOND LENGTH                     (A) : NULL  ; NULL
REMARK   3    ANGLE DISTANCE                  (A) : NULL  ; NULL
REMARK   3    INTRAPLANAR 1-4 DISTANCE        (A) : NULL  ; NULL
REMARK   3    H-BOND OR METAL COORDINATION    (A) : NULL  ; NULL
REMARK   3
REMARK   3   PLANE RESTRAINT                  (A) : NULL  ; NULL
REMARK   3   CHIRAL-CENTER RESTRAINT       (A**3) : NULL  ; NULL
REMARK   3
REMARK   3   NON-BONDED CONTACT RESTRAINTS.
REMARK   3    SINGLE TORSION                  (A) : NULL  ; NULL
REMARK   3    MULTIPLE TORSION                (A) : NULL  ; NULL
REMARK   3    H-BOND (X...Y)                  (A) : NULL  ; NULL
REMARK   3    H-BOND (X-H...Y)                (A) : NULL  ; NULL
REMARK   3
REMARK   3   CONFORMATIONAL TORSION ANGLE RESTRAINTS.
REMARK   3    SPECIFIED                 (DEGREES) : NULL  ; NULL
REMARK   3    PLANAR                    (DEGREES) : NULL  ; NULL
REMARK   3    STAGGERED                 (DEGREES) : NULL  ; NULL
REMARK   3    TRANSVERSE                (DEGREES) : NULL  ; NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN USED IF PRESENT IN
REMARK   3  THE INPUT
REMARK   4
REMARK   4 8ZRG COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 05-JUN-24.
REMARK 100 THE DEPOSITION ID IS D_1300048410.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 22-MAY-24
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SOLEIL
REMARK 200  BEAMLINE                       : PROXIMA 1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.987
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER X 16M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XSCALE
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 12939
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.750
REMARK 200  RESOLUTION RANGE LOW       (A) : 49.070
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0
REMARK 200  DATA REDUNDANCY                : 20.00
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 12.2600
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.75
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.85
REMARK 200  COMPLETENESS FOR SHELL     (%) : 91.3
REMARK 200  DATA REDUNDANCY IN SHELL       : 15.00
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 1.910
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: AF-Q9FG13-F1
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 57.21
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.87
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.056 M SODIUM PHOSPHATE MONOBASIC
REMARK 280  MONOHYDRATE, 1.344 M POTASSIUM PHOSPHATE DIBASIC, PH 8.2, VAPOR
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 298K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 41 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z+1/2
REMARK 290       3555   -Y+1/2,X+1/2,Z+1/4
REMARK 290       4555   Y+1/2,-X+1/2,Z+3/4
REMARK 290       5555   -X+1/2,Y+1/2,-Z+1/4
REMARK 290       6555   X+1/2,-Y+1/2,-Z+3/4
REMARK 290       7555   Y,X,-Z
REMARK 290       8555   -Y,-X,-Z+1/2
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       60.44400
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       41.90750
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       41.90750
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       30.22200
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       41.90750
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       41.90750
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       90.66600
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       41.90750
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       41.90750
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       30.22200
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       41.90750
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       41.90750
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       90.66600
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       60.44400
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 5630 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 24940 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -27.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350   BIOMT1   2  0.000000 -1.000000  0.000000        0.00000
REMARK 350   BIOMT2   2 -1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000       60.44400
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A 169     -120.86     48.19
REMARK 500    LEU A 220      -48.36   -134.18
REMARK 500    ARG A 240        1.39    -69.95
REMARK 500    PHE A 248       71.17   -151.70
REMARK 500    LEU A 255       -4.75     64.54
REMARK 500    ASN A 299       14.00     59.37
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        RMS     TYPE
REMARK 500    ARG A  92         0.16    SIDE CHAIN
REMARK 500    ARG A 162         0.08    SIDE CHAIN
REMARK 500    ARG A 193         0.12    SIDE CHAIN
REMARK 500    ARG A 291         0.29    SIDE CHAIN
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A 429        DISTANCE =  6.42 ANGSTROMS
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 8ZR6   RELATED DB: PDB
DBREF  8ZRG A   14   329  UNP    Q9FG13   CXE15_ARATH     14    329
SEQADV 8ZRG SER A   14  UNP  Q9FG13    CYS    14 ENGINEERED MUTATION
SEQRES   1 A  316  SER MET GLY LEU LEU GLN LEU LEU SER ASN GLY THR VAL
SEQRES   2 A  316  LEU ARG SER GLU SER ILE ASP LEU ILE THR GLN GLN ILE
SEQRES   3 A  316  PRO PHE LYS ASN ASN GLN THR VAL LEU PHE LYS ASP SER
SEQRES   4 A  316  ILE TYR HIS LYS PRO ASN ASN LEU HIS LEU ARG LEU TYR
SEQRES   5 A  316  LYS PRO ILE SER ALA SER ASN ARG THR ALA LEU PRO VAL
SEQRES   6 A  316  VAL VAL PHE PHE HIS GLY GLY GLY PHE CYS PHE GLY SER
SEQRES   7 A  316  ARG SER TRP PRO HIS PHE HIS ASN PHE CYS LEU THR LEU
SEQRES   8 A  316  ALA SER SER LEU ASN ALA LEU VAL VAL SER PRO ASP TYR
SEQRES   9 A  316  ARG LEU ALA PRO GLU HIS ARG LEU PRO ALA ALA PHE GLU
SEQRES  10 A  316  ASP ALA GLU ALA VAL LEU THR TRP LEU TRP ASP GLN ALA
SEQRES  11 A  316  VAL SER ASP GLY VAL ASN HIS TRP PHE GLU ASP GLY THR
SEQRES  12 A  316  ASP VAL ASP PHE ASP ARG VAL PHE VAL VAL GLY ASP SER
SEQRES  13 A  316  SER GLY GLY ASN ILE ALA HIS GLN LEU ALA VAL ARG PHE
SEQRES  14 A  316  GLY SER GLY SER ILE GLU LEU THR PRO VAL ARG VAL ARG
SEQRES  15 A  316  GLY TYR VAL LEU MET GLY PRO PHE PHE GLY GLY GLU GLU
SEQRES  16 A  316  ARG THR ASN SER GLU ASN GLY PRO SER GLU ALA LEU LEU
SEQRES  17 A  316  SER LEU ASP LEU LEU ASP LYS PHE TRP ARG LEU SER LEU
SEQRES  18 A  316  PRO ASN GLY ALA THR ARG ASP HIS HIS MET ALA ASN PRO
SEQRES  19 A  316  PHE GLY PRO THR SER PRO THR LEU GLU SER ILE SER LEU
SEQRES  20 A  316  GLU PRO MET LEU VAL ILE VAL GLY GLY SER GLU LEU LEU
SEQRES  21 A  316  ARG ASP ARG ALA LYS GLU TYR ALA TYR LYS LEU LYS LYS
SEQRES  22 A  316  MET GLY GLY LYS ARG VAL ASP TYR ILE GLU PHE GLU ASN
SEQRES  23 A  316  LYS GLU HIS GLY PHE TYR SER ASN TYR PRO SER SER GLU
SEQRES  24 A  316  ALA ALA GLU GLN VAL LEU ARG ILE ILE GLY ASP PHE MET
SEQRES  25 A  316  ASN ASN LEU SER
FORMUL   2  HOH   *29(H2 O)
HELIX    1 AA1 GLY A   16  GLY A   24  1                                   9
HELIX    2 AA2 SER A   69  ASN A   72  5                                   4
HELIX    3 AA3 TRP A   94  ASN A  109  1                                  16
HELIX    4 AA4 PRO A  126  VAL A  144  1                                  19
HELIX    5 AA5 ASP A  146  TRP A  151  5                                   6
HELIX    6 AA6 SER A  169  SER A  184  1                                  16
HELIX    7 AA7 THR A  210  GLY A  215  1                                   6
HELIX    8 AA8 SER A  222  LEU A  234  1                                  13
HELIX    9 AA9 LEU A  273  MET A  287  1                                  15
HELIX   10 AB1 GLY A  303  TYR A  308  1                                   6
HELIX   11 AB2 SER A  311  ASN A  327  1                                  17
SHEET    1 AA1 8 VAL A  47  HIS A  55  0
SHEET    2 AA1 8 LEU A  60  PRO A  67 -1  O  LYS A  66   N  LEU A  48
SHEET    3 AA1 8 LEU A 111  PRO A 115 -1  O  SER A 114   N  ARG A  63
SHEET    4 AA1 8 LEU A  76  PHE A  82  1  N  VAL A  79   O  VAL A 113
SHEET    5 AA1 8 VAL A 158  ASP A 168  1  O  ARG A 162   N  VAL A  78
SHEET    6 AA1 8 VAL A 194  MET A 200  1  O  MET A 200   N  GLY A 167
SHEET    7 AA1 8 MET A 263  GLY A 268  1  O  LEU A 264   N  LEU A 199
SHEET    8 AA1 8 VAL A 292  PHE A 297  1  O  ILE A 295   N  VAL A 265
CISPEP   1 ALA A  120    PRO A  121          0        19.10
CISPEP   2 LEU A  125    PRO A  126          0        19.62
CISPEP   3 THR A  190    PRO A  191          0        30.00
CRYST1   83.815   83.815  120.888  90.00  90.00  90.00 P 41 21 2     8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.011931  0.000000  0.000000        0.00000
SCALE2      0.000000  0.011931  0.000000        0.00000
SCALE3      0.000000  0.000000  0.008272        0.00000
TER    2492      SER A 329
MASTER      272    0    0   11    8    0    0    6 2520    1    0   25
END