longtext: 9EBF-pdb

content
HEADER    HYDROLASE                               12-NOV-24   9EBF
TITLE     FPHE, STAPHYLOCOCCUS AUREUS FLUOROPHOSPHONATE-BINDING SERINE
TITLE    2 HYDROLASES E, BOROLANE-BASED COMPOUND Q41 BOUND IN CRYSTAL FORM 6
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: UNCHARACTERIZED HYDROLASE SAUSA300_2518;
COMPND   3 CHAIN: A, B, C, D;
COMPND   4 EC: 3.-.-.-;
COMPND   5 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: STAPHYLOCOCCUS AUREUS SUBSP. AUREUS USA300;
SOURCE   3 ORGANISM_TAXID: 367830;
SOURCE   4 GENE: SAUSA300_2518;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008
KEYWDS    FPHE, STAPHYLOCOCCUS AUREUS, S. AUREUS, FLUOROPHOSPHONATE-BINDING,
KEYWDS   2 SERINE HYDROLASES, LIPASE, HYDROLASE, BORON, SERINE-BORON,
KEYWDS   3 HISTIDINE-BORON
EXPDTA    X-RAY DIFFRACTION
AUTHOR    M.FELLNER
REVDAT   1   21-JAN-26 9EBF    0
JRNL        AUTH   M.FELLNER
JRNL        TITL   FPHE, STAPHYLOCOCCUS AUREUS FLUOROPHOSPHONATE-BINDING SERINE
JRNL        TITL 2 HYDROLASES E, BOROLANE-BASED COMPOUND Q41 BOUND IN CRYSTAL
JRNL        TITL 3 FORM 6
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.50 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (1.20.1_4487: ???)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.50
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 47.47
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.9
REMARK   3   NUMBER OF REFLECTIONS             : 54834
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.185
REMARK   3   R VALUE            (WORKING SET) : 0.183
REMARK   3   FREE R VALUE                     : 0.222
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.050
REMARK   3   FREE R VALUE TEST SET COUNT      : 2769
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 47.4700 -  6.7800    1.00     2752   111  0.1319 0.1446
REMARK   3     2  6.7700 -  5.3900    1.00     2635   155  0.1725 0.1975
REMARK   3     3  5.3800 -  4.7000    1.00     2619   144  0.1439 0.1931
REMARK   3     4  4.7000 -  4.2700    1.00     2601   162  0.1422 0.1791
REMARK   3     5  4.2700 -  3.9700    1.00     2646   111  0.1560 0.1966
REMARK   3     6  3.9700 -  3.7300    1.00     2559   171  0.1674 0.2284
REMARK   3     7  3.7300 -  3.5500    1.00     2643   116  0.1821 0.2253
REMARK   3     8  3.5500 -  3.3900    1.00     2602   136  0.2188 0.2547
REMARK   3     9  3.3900 -  3.2600    1.00     2618   116  0.2187 0.2516
REMARK   3    10  3.2600 -  3.1500    1.00     2591   131  0.2342 0.2944
REMARK   3    11  3.1500 -  3.0500    1.00     2614   140  0.2214 0.2766
REMARK   3    12  3.0500 -  2.9600    1.00     2551   168  0.2367 0.2825
REMARK   3    13  2.9600 -  2.8900    1.00     2575   129  0.2528 0.3120
REMARK   3    14  2.8900 -  2.8200    1.00     2600   158  0.2702 0.2917
REMARK   3    15  2.8100 -  2.7500    1.00     2601   119  0.2753 0.3145
REMARK   3    16  2.7500 -  2.6900    1.00     2574   158  0.2507 0.3085
REMARK   3    17  2.6900 -  2.6400    1.00     2584   123  0.2541 0.2404
REMARK   3    18  2.6400 -  2.5900    1.00     2552   147  0.2653 0.2747
REMARK   3    19  2.5900 -  2.5400    1.00     2632   136  0.2736 0.3498
REMARK   3    20  2.5400 -  2.5000    0.97     2516   138  0.3084 0.3258
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.10
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.340
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 24.710
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.009           9064
REMARK   3   ANGLE     :  1.008          12308
REMARK   3   CHIRALITY :  0.057           1332
REMARK   3   PLANARITY :  0.010           1612
REMARK   3   DIHEDRAL  : 16.973           3376
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 14
REMARK   3   TLS GROUP : 1
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 1 THROUGH 115 )
REMARK   3    ORIGIN FOR THE GROUP (A):  10.6745 -17.5610 -80.8968
REMARK   3    T TENSOR
REMARK   3      T11:   0.5329 T22:   0.6977
REMARK   3      T33:   0.6142 T12:  -0.1080
REMARK   3      T13:   0.1254 T23:  -0.2924
REMARK   3    L TENSOR
REMARK   3      L11:   4.6741 L22:   2.3181
REMARK   3      L33:   2.6053 L12:   0.1588
REMARK   3      L13:   0.5975 L23:  -0.0245
REMARK   3    S TENSOR
REMARK   3      S11:   0.1321 S12:  -1.0867 S13:   0.6342
REMARK   3      S21:   0.3272 S22:  -0.0002 S23:   0.0586
REMARK   3      S31:  -0.6313 S32:  -0.0075 S33:  -0.1063
REMARK   3   TLS GROUP : 2
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 116 THROUGH 152 )
REMARK   3    ORIGIN FOR THE GROUP (A):  -0.1898 -29.5336 -88.4484
REMARK   3    T TENSOR
REMARK   3      T11:   0.4219 T22:   0.6555
REMARK   3      T33:   0.5454 T12:  -0.1215
REMARK   3      T13:   0.0213 T23:  -0.0689
REMARK   3    L TENSOR
REMARK   3      L11:   3.7084 L22:   0.4654
REMARK   3      L33:   2.3807 L12:  -0.8978
REMARK   3      L13:   1.2690 L23:  -1.1781
REMARK   3    S TENSOR
REMARK   3      S11:   0.2229 S12:  -0.8482 S13:   0.1980
REMARK   3      S21:  -0.1243 S22:   0.0746 S23:   0.5967
REMARK   3      S31:   0.0523 S32:  -0.5238 S33:  -0.3222
REMARK   3   TLS GROUP : 3
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 153 THROUGH 200 )
REMARK   3    ORIGIN FOR THE GROUP (A):  18.7095 -50.6548 -75.0095
REMARK   3    T TENSOR
REMARK   3      T11:   0.5981 T22:   0.8079
REMARK   3      T33:   0.4827 T12:  -0.2497
REMARK   3      T13:  -0.1057 T23:   0.1199
REMARK   3    L TENSOR
REMARK   3      L11:   4.4370 L22:   3.9560
REMARK   3      L33:   3.7990 L12:  -1.6031
REMARK   3      L13:   2.0429 L23:  -1.0580
REMARK   3    S TENSOR
REMARK   3      S11:   0.3795 S12:  -0.3520 S13:  -0.4974
REMARK   3      S21:  -0.1164 S22:   0.0869 S23:   0.2499
REMARK   3      S31:   0.5038 S32:  -0.3275 S33:  -0.4423
REMARK   3   TLS GROUP : 4
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 201 THROUGH 276 )
REMARK   3    ORIGIN FOR THE GROUP (A):  34.8174 -61.1804 -61.1253
REMARK   3    T TENSOR
REMARK   3      T11:   0.6808 T22:   0.8330
REMARK   3      T33:   0.8119 T12:  -0.1247
REMARK   3      T13:  -0.3044 T23:   0.1266
REMARK   3    L TENSOR
REMARK   3      L11:   1.2341 L22:   1.7887
REMARK   3      L33:   2.5036 L12:  -0.3370
REMARK   3      L13:   0.0908 L23:  -0.3410
REMARK   3    S TENSOR
REMARK   3      S11:   0.3140 S12:  -0.1592 S13:  -0.2182
REMARK   3      S21:   0.4512 S22:  -0.1284 S23:  -0.5386
REMARK   3      S31:   0.5259 S32:   0.5194 S33:  -0.2063
REMARK   3   TLS GROUP : 5
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 1 THROUGH 136 )
REMARK   3    ORIGIN FOR THE GROUP (A):  30.1744 -47.5533 -56.5513
REMARK   3    T TENSOR
REMARK   3      T11:   0.6241 T22:   0.8227
REMARK   3      T33:   0.5695 T12:  -0.2622
REMARK   3      T13:  -0.1443 T23:   0.0537
REMARK   3    L TENSOR
REMARK   3      L11:   2.0568 L22:   1.7294
REMARK   3      L33:   2.5414 L12:  -0.5484
REMARK   3      L13:   0.3731 L23:  -0.2143
REMARK   3    S TENSOR
REMARK   3      S11:   0.2991 S12:  -0.4093 S13:   0.0274
REMARK   3      S21:   0.4696 S22:  -0.0995 S23:  -0.1596
REMARK   3      S31:   0.1216 S32:   0.2239 S33:  -0.2277
REMARK   3   TLS GROUP : 6
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 137 THROUGH 200 )
REMARK   3    ORIGIN FOR THE GROUP (A):  22.3385 -41.1192 -85.8205
REMARK   3    T TENSOR
REMARK   3      T11:   0.5188 T22:   0.6313
REMARK   3      T33:   0.4524 T12:  -0.1692
REMARK   3      T13:  -0.0120 T23:   0.0499
REMARK   3    L TENSOR
REMARK   3      L11:   4.2950 L22:   4.2332
REMARK   3      L33:   1.9233 L12:  -3.8531
REMARK   3      L13:   3.0086 L23:  -2.1190
REMARK   3    S TENSOR
REMARK   3      S11:   0.5781 S12:  -0.1169 S13:  -0.0236
REMARK   3      S21:  -0.3754 S22:  -0.4983 S23:  -0.0957
REMARK   3      S31:   0.2717 S32:   0.2783 S33:  -0.0616
REMARK   3   TLS GROUP : 7
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 201 THROUGH 276 )
REMARK   3    ORIGIN FOR THE GROUP (A):   2.2679 -20.9149 -95.5293
REMARK   3    T TENSOR
REMARK   3      T11:   0.4676 T22:   0.3268
REMARK   3      T33:   0.5065 T12:  -0.0125
REMARK   3      T13:   0.0779 T23:  -0.0768
REMARK   3    L TENSOR
REMARK   3      L11:   6.2489 L22:   1.1729
REMARK   3      L33:   3.4554 L12:   0.8552
REMARK   3      L13:   0.7427 L23:  -0.1635
REMARK   3    S TENSOR
REMARK   3      S11:   0.1543 S12:  -0.4427 S13:   0.7961
REMARK   3      S21:  -0.1940 S22:  -0.0165 S23:   0.2037
REMARK   3      S31:  -0.4144 S32:  -0.1593 S33:  -0.1432
REMARK   3   TLS GROUP : 8
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 1 THROUGH 75 )
REMARK   3    ORIGIN FOR THE GROUP (A):  58.2021 -17.6144 -87.1087
REMARK   3    T TENSOR
REMARK   3      T11:   0.5841 T22:   0.9920
REMARK   3      T33:   0.4836 T12:  -0.3366
REMARK   3      T13:   0.0981 T23:  -0.1436
REMARK   3    L TENSOR
REMARK   3      L11:   5.4378 L22:   2.9755
REMARK   3      L33:   3.4238 L12:  -0.2346
REMARK   3      L13:  -0.2760 L23:   0.5239
REMARK   3    S TENSOR
REMARK   3      S11:   0.6388 S12:  -1.5855 S13:   0.2325
REMARK   3      S21:   0.4800 S22:  -0.6673 S23:  -0.1838
REMARK   3      S31:  -0.0430 S32:   0.4382 S33:   0.0941
REMARK   3   TLS GROUP : 9
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 76 THROUGH 152 )
REMARK   3    ORIGIN FOR THE GROUP (A):  45.9141 -13.3723 -84.1297
REMARK   3    T TENSOR
REMARK   3      T11:   0.6921 T22:   1.1200
REMARK   3      T33:   0.7671 T12:  -0.3847
REMARK   3      T13:   0.2429 T23:  -0.4821
REMARK   3    L TENSOR
REMARK   3      L11:   1.6525 L22:   3.8755
REMARK   3      L33:   1.7150 L12:   0.4530
REMARK   3      L13:  -1.5497 L23:   0.5450
REMARK   3    S TENSOR
REMARK   3      S11:   0.7101 S12:  -1.6158 S13:   1.1165
REMARK   3      S21:   0.4475 S22:  -0.5736 S23:   0.4663
REMARK   3      S31:  -0.3329 S32:   0.3922 S33:  -0.0303
REMARK   3   TLS GROUP : 10
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 153 THROUGH 200 )
REMARK   3    ORIGIN FOR THE GROUP (A):  42.0613 -43.9395 -98.0877
REMARK   3    T TENSOR
REMARK   3      T11:   0.4577 T22:   0.5011
REMARK   3      T33:   0.4485 T12:  -0.1117
REMARK   3      T13:   0.0311 T23:   0.0393
REMARK   3    L TENSOR
REMARK   3      L11:   3.7102 L22:   8.2471
REMARK   3      L33:   3.4069 L12:  -1.1724
REMARK   3      L13:  -0.4293 L23:   0.5265
REMARK   3    S TENSOR
REMARK   3      S11:   0.0768 S12:  -0.1331 S13:  -0.1976
REMARK   3      S21:  -0.0120 S22:  -0.2188 S23:   0.6625
REMARK   3      S31:   0.2372 S32:   0.1552 S33:   0.1440
REMARK   3   TLS GROUP : 11
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 201 THROUGH 276 )
REMARK   3    ORIGIN FOR THE GROUP (A):  51.1615 -65.8122-102.0702
REMARK   3    T TENSOR
REMARK   3      T11:   0.7202 T22:   0.4598
REMARK   3      T33:   0.6924 T12:   0.0730
REMARK   3      T13:   0.0650 T23:   0.0098
REMARK   3    L TENSOR
REMARK   3      L11:   3.4017 L22:   4.4974
REMARK   3      L33:   4.1359 L12:   1.1394
REMARK   3      L13:   0.0839 L23:   0.2514
REMARK   3    S TENSOR
REMARK   3      S11:  -0.0753 S12:  -0.0002 S13:  -0.8306
REMARK   3      S21:  -0.7175 S22:   0.0295 S23:  -0.5519
REMARK   3      S31:   1.0454 S32:   0.3945 S33:   0.0553
REMARK   3   TLS GROUP : 12
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 1 THROUGH 152 )
REMARK   3    ORIGIN FOR THE GROUP (A):  57.1476 -56.5465 -93.6759
REMARK   3    T TENSOR
REMARK   3      T11:   0.4068 T22:   0.6214
REMARK   3      T33:   0.6882 T12:   0.0340
REMARK   3      T13:   0.0205 T23:   0.0846
REMARK   3    L TENSOR
REMARK   3      L11:   2.1529 L22:   3.6091
REMARK   3      L33:   3.3923 L12:   0.7019
REMARK   3      L13:  -0.6787 L23:  -0.3209
REMARK   3    S TENSOR
REMARK   3      S11:   0.0757 S12:  -0.3806 S13:  -0.4768
REMARK   3      S21:  -0.1129 S22:  -0.2163 S23:  -0.9068
REMARK   3      S31:   0.3046 S32:   0.8043 S33:   0.1061
REMARK   3   TLS GROUP : 13
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 153 THROUGH 200 )
REMARK   3    ORIGIN FOR THE GROUP (A):  46.6338 -26.2500-101.3945
REMARK   3    T TENSOR
REMARK   3      T11:   0.5346 T22:   0.4713
REMARK   3      T33:   0.3656 T12:  -0.1517
REMARK   3      T13:   0.0676 T23:  -0.0207
REMARK   3    L TENSOR
REMARK   3      L11:   4.9589 L22:   7.7544
REMARK   3      L33:   2.0307 L12:   0.3824
REMARK   3      L13:   0.0035 L23:   0.1115
REMARK   3    S TENSOR
REMARK   3      S11:   0.1328 S12:  -0.0782 S13:   0.2528
REMARK   3      S21:  -0.7867 S22:  -0.1446 S23:  -0.0741
REMARK   3      S31:  -0.1348 S32:   0.1682 S33:  -0.0088
REMARK   3   TLS GROUP : 14
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 201 THROUGH 276 )
REMARK   3    ORIGIN FOR THE GROUP (A):  44.4225  -5.0174 -90.9682
REMARK   3    T TENSOR
REMARK   3      T11:   0.8008 T22:   0.7805
REMARK   3      T33:   1.2222 T12:  -0.2868
REMARK   3      T13:   0.3043 T23:  -0.4716
REMARK   3    L TENSOR
REMARK   3      L11:   2.4859 L22:   2.6164
REMARK   3      L33:   1.2142 L12:  -0.5462
REMARK   3      L13:   0.7371 L23:   1.0926
REMARK   3    S TENSOR
REMARK   3      S11:   0.5638 S12:  -1.1207 S13:   1.7496
REMARK   3      S21:   0.0054 S22:  -0.4667 S23:   0.8101
REMARK   3      S31:  -0.5845 S32:   0.0520 S33:  -0.0935
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 9EBF COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 13-NOV-24.
REMARK 100 THE DEPOSITION ID IS D_1000290113.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 27-APR-23
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 5.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : AUSTRALIAN SYNCHROTRON
REMARK 200  BEAMLINE                       : MX2
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.954
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER X 16M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 54846
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.500
REMARK 200  RESOLUTION RANGE LOW       (A) : 47.470
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9
REMARK 200  DATA REDUNDANCY                : 31.20
REMARK 200  R MERGE                    (I) : 0.12200
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 23.7000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.50
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.57
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : 30.50
REMARK 200  R MERGE FOR SHELL          (I) : 2.17900
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 61.40
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.19
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 16 UL 11.2 MG/ML FPHE (10 MM HEPES PH
REMARK 280  7.5, 100 MM NACL) WERE MIXED WITH 6 UL Q41 (50 MM IN DMSO) AND
REMARK 280  INCUBATED AT 18C OVERNIGHT. 0.15 UL FPHE-Q41 SOLUTION WAS MIXED
REMARK 280  WITH 0.3 UL OF RESERVOIR SOLUTION. SITTING DROP RESERVOIR
REMARK 280  CONTAINED 25 UL OF 180 MM POTASSIUM THIOCYANATE, 100 MM SODIUM
REMARK 280  ACETATE PH 5.5, 22.5% PEG 2000 MME. CRYSTAL WAS FROZEN IN A
REMARK 280  SOLUTION OF ~25% GLYCEROL, 75% RESERVOIR., VAPOR DIFFUSION,
REMARK 280  SITTING DROP, TEMPERATURE 291.15K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: I 2 3
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z
REMARK 290       3555   -X,Y,-Z
REMARK 290       4555   X,-Y,-Z
REMARK 290       5555   Z,X,Y
REMARK 290       6555   Z,-X,-Y
REMARK 290       7555   -Z,-X,Y
REMARK 290       8555   -Z,X,-Y
REMARK 290       9555   Y,Z,X
REMARK 290      10555   -Y,Z,-X
REMARK 290      11555   Y,-Z,-X
REMARK 290      12555   -Y,-Z,X
REMARK 290      13555   X+1/2,Y+1/2,Z+1/2
REMARK 290      14555   -X+1/2,-Y+1/2,Z+1/2
REMARK 290      15555   -X+1/2,Y+1/2,-Z+1/2
REMARK 290      16555   X+1/2,-Y+1/2,-Z+1/2
REMARK 290      17555   Z+1/2,X+1/2,Y+1/2
REMARK 290      18555   Z+1/2,-X+1/2,-Y+1/2
REMARK 290      19555   -Z+1/2,-X+1/2,Y+1/2
REMARK 290      20555   -Z+1/2,X+1/2,-Y+1/2
REMARK 290      21555   Y+1/2,Z+1/2,X+1/2
REMARK 290      22555   -Y+1/2,Z+1/2,-X+1/2
REMARK 290      23555   Y+1/2,-Z+1/2,-X+1/2
REMARK 290      24555   -Y+1/2,-Z+1/2,X+1/2
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   5  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY2   5  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   5  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY1   6  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY2   6 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   6  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY1   7  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY2   7 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   7  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY1   8  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY2   8  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY3   8  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY1   9  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2   9  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY3   9  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY1  10  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2  10  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY3  10 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY1  11  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY2  11  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY3  11 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY1  12  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY2  12  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY3  12  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY1  13  1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY2  13  0.000000  1.000000  0.000000      106.14300
REMARK 290   SMTRY3  13  0.000000  0.000000  1.000000      106.14300
REMARK 290   SMTRY1  14 -1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY2  14  0.000000 -1.000000  0.000000      106.14300
REMARK 290   SMTRY3  14  0.000000  0.000000  1.000000      106.14300
REMARK 290   SMTRY1  15 -1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY2  15  0.000000  1.000000  0.000000      106.14300
REMARK 290   SMTRY3  15  0.000000  0.000000 -1.000000      106.14300
REMARK 290   SMTRY1  16  1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY2  16  0.000000 -1.000000  0.000000      106.14300
REMARK 290   SMTRY3  16  0.000000  0.000000 -1.000000      106.14300
REMARK 290   SMTRY1  17  0.000000  0.000000  1.000000      106.14300
REMARK 290   SMTRY2  17  1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY3  17  0.000000  1.000000  0.000000      106.14300
REMARK 290   SMTRY1  18  0.000000  0.000000  1.000000      106.14300
REMARK 290   SMTRY2  18 -1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY3  18  0.000000 -1.000000  0.000000      106.14300
REMARK 290   SMTRY1  19  0.000000  0.000000 -1.000000      106.14300
REMARK 290   SMTRY2  19 -1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY3  19  0.000000  1.000000  0.000000      106.14300
REMARK 290   SMTRY1  20  0.000000  0.000000 -1.000000      106.14300
REMARK 290   SMTRY2  20  1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY3  20  0.000000 -1.000000  0.000000      106.14300
REMARK 290   SMTRY1  21  0.000000  1.000000  0.000000      106.14300
REMARK 290   SMTRY2  21  0.000000  0.000000  1.000000      106.14300
REMARK 290   SMTRY3  21  1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY1  22  0.000000 -1.000000  0.000000      106.14300
REMARK 290   SMTRY2  22  0.000000  0.000000  1.000000      106.14300
REMARK 290   SMTRY3  22 -1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY1  23  0.000000  1.000000  0.000000      106.14300
REMARK 290   SMTRY2  23  0.000000  0.000000 -1.000000      106.14300
REMARK 290   SMTRY3  23 -1.000000  0.000000  0.000000      106.14300
REMARK 290   SMTRY1  24  0.000000 -1.000000  0.000000      106.14300
REMARK 290   SMTRY2  24  0.000000  0.000000 -1.000000      106.14300
REMARK 290   SMTRY3  24  1.000000  0.000000  0.000000      106.14300
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 12660 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 21880 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -95.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 12790 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 22190 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -97.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     GLY A    -2
REMARK 465     GLY B    -2
REMARK 465     GLY C    -2
REMARK 465     GLY D    -2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OG   SER B   103     O1   XPU B   301              2.01
REMARK 500   NE2  HIS C   257     OG   SER D   103              2.14
REMARK 500   OG   SER C   103     NE2  HIS D   257              2.15
REMARK 500   OG   SER A   103     NE2  HIS B   257              2.17
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A 103     -132.19     57.95
REMARK 500    PRO A 129       30.59    -96.50
REMARK 500    ASP A 136       31.18    -97.03
REMARK 500    THR A 153      -72.37   -108.55
REMARK 500    GLU A 201      -54.76   -125.02
REMARK 500    GLN B   7      108.50    -48.59
REMARK 500    SER B 103     -131.51     57.44
REMARK 500    GLU B 127       70.09     48.39
REMARK 500    THR B 153      -73.40   -120.80
REMARK 500    SER C 103     -130.26     53.13
REMARK 500    TYR C 116       58.08   -145.09
REMARK 500    ASP C 136       36.41    -97.73
REMARK 500    THR C 153      -72.45   -114.63
REMARK 500    TYR C 218       43.45   -141.49
REMARK 500    ALA D   9      149.21   -174.80
REMARK 500    SER D 103     -130.62     57.80
REMARK 500    ASP D 136       38.56    -98.76
REMARK 500    GLU D 201      -55.46   -122.16
REMARK 500    TYR D 218       36.75    -92.25
REMARK 500
REMARK 500 REMARK: NULL
DBREF  9EBF A    1   276  UNP    Q2FDS6   Y2518_STAA3      1    276
DBREF  9EBF B    1   276  UNP    Q2FDS6   Y2518_STAA3      1    276
DBREF  9EBF C    1   276  UNP    Q2FDS6   Y2518_STAA3      1    276
DBREF  9EBF D    1   276  UNP    Q2FDS6   Y2518_STAA3      1    276
SEQADV 9EBF GLY A   -2  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF PRO A   -1  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF GLY A    0  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF GLY B   -2  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF PRO B   -1  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF GLY B    0  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF GLY C   -2  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF PRO C   -1  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF GLY C    0  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF GLY D   -2  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF PRO D   -1  UNP  Q2FDS6              EXPRESSION TAG
SEQADV 9EBF GLY D    0  UNP  Q2FDS6              EXPRESSION TAG
SEQRES   1 A  279  GLY PRO GLY MET GLU THR LEU GLU LEU GLN GLY ALA LYS
SEQRES   2 A  279  LEU ARG TYR HIS GLN VAL GLY GLN GLY PRO VAL LEU ILE
SEQRES   3 A  279  PHE ILE PRO GLY ALA ASN GLY THR GLY ASP ILE PHE LEU
SEQRES   4 A  279  PRO LEU ALA GLU GLN LEU LYS ASP HIS PHE THR VAL VAL
SEQRES   5 A  279  ALA VAL ASP ARG ARG ASP TYR GLY GLU SER GLU LEU THR
SEQRES   6 A  279  GLU PRO LEU PRO ASP SER ALA SER ASN PRO ASP SER ASP
SEQRES   7 A  279  TYR ARG VAL LYS ARG ASP ALA GLN ASP ILE ALA GLU LEU
SEQRES   8 A  279  ALA LYS SER LEU SER ASP GLU PRO VAL TYR ILE LEU GLY
SEQRES   9 A  279  SER SER SER GLY SER ILE VAL ALA MET HIS VAL LEU LYS
SEQRES  10 A  279  ASP TYR PRO GLU VAL VAL LYS LYS ILE ALA PHE HIS GLU
SEQRES  11 A  279  PRO PRO ILE ASN THR PHE LEU PRO ASP SER THR TYR TRP
SEQRES  12 A  279  LYS ASP LYS ASN ASP ASP ILE VAL HIS GLN ILE LEU THR
SEQRES  13 A  279  GLU GLY LEU GLU LYS GLY MET LYS THR PHE GLY GLU THR
SEQRES  14 A  279  LEU ASN ILE ALA PRO ILE ASP ALA LYS MET MET SER GLN
SEQRES  15 A  279  PRO ALA ASP THR GLU GLU GLY ARG ILE GLU GLN TYR LYS
SEQRES  16 A  279  ARG THR MET PHE TRP LEU GLU PHE GLU ILE ARG GLN TYR
SEQRES  17 A  279  THR HIS SER ASN ILE THR LEU ASP ASP PHE THR LYS TYR
SEQRES  18 A  279  SER ASP LYS ILE THR LEU LEU ASN GLY THR ASP SER ARG
SEQRES  19 A  279  GLY SER PHE PRO GLN ASP VAL ASN PHE TYR ILE ASN LYS
SEQRES  20 A  279  GLU THR GLY ILE PRO ILE VAL ASP ILE PRO GLY GLY HIS
SEQRES  21 A  279  LEU GLY TYR ILE GLN LYS PRO GLU GLY PHE ALA ASP VAL
SEQRES  22 A  279  LEU LEU ASN MET TRP GLY
SEQRES   1 B  279  GLY PRO GLY MET GLU THR LEU GLU LEU GLN GLY ALA LYS
SEQRES   2 B  279  LEU ARG TYR HIS GLN VAL GLY GLN GLY PRO VAL LEU ILE
SEQRES   3 B  279  PHE ILE PRO GLY ALA ASN GLY THR GLY ASP ILE PHE LEU
SEQRES   4 B  279  PRO LEU ALA GLU GLN LEU LYS ASP HIS PHE THR VAL VAL
SEQRES   5 B  279  ALA VAL ASP ARG ARG ASP TYR GLY GLU SER GLU LEU THR
SEQRES   6 B  279  GLU PRO LEU PRO ASP SER ALA SER ASN PRO ASP SER ASP
SEQRES   7 B  279  TYR ARG VAL LYS ARG ASP ALA GLN ASP ILE ALA GLU LEU
SEQRES   8 B  279  ALA LYS SER LEU SER ASP GLU PRO VAL TYR ILE LEU GLY
SEQRES   9 B  279  SER SER SER GLY SER ILE VAL ALA MET HIS VAL LEU LYS
SEQRES  10 B  279  ASP TYR PRO GLU VAL VAL LYS LYS ILE ALA PHE HIS GLU
SEQRES  11 B  279  PRO PRO ILE ASN THR PHE LEU PRO ASP SER THR TYR TRP
SEQRES  12 B  279  LYS ASP LYS ASN ASP ASP ILE VAL HIS GLN ILE LEU THR
SEQRES  13 B  279  GLU GLY LEU GLU LYS GLY MET LYS THR PHE GLY GLU THR
SEQRES  14 B  279  LEU ASN ILE ALA PRO ILE ASP ALA LYS MET MET SER GLN
SEQRES  15 B  279  PRO ALA ASP THR GLU GLU GLY ARG ILE GLU GLN TYR LYS
SEQRES  16 B  279  ARG THR MET PHE TRP LEU GLU PHE GLU ILE ARG GLN TYR
SEQRES  17 B  279  THR HIS SER ASN ILE THR LEU ASP ASP PHE THR LYS TYR
SEQRES  18 B  279  SER ASP LYS ILE THR LEU LEU ASN GLY THR ASP SER ARG
SEQRES  19 B  279  GLY SER PHE PRO GLN ASP VAL ASN PHE TYR ILE ASN LYS
SEQRES  20 B  279  GLU THR GLY ILE PRO ILE VAL ASP ILE PRO GLY GLY HIS
SEQRES  21 B  279  LEU GLY TYR ILE GLN LYS PRO GLU GLY PHE ALA ASP VAL
SEQRES  22 B  279  LEU LEU ASN MET TRP GLY
SEQRES   1 C  279  GLY PRO GLY MET GLU THR LEU GLU LEU GLN GLY ALA LYS
SEQRES   2 C  279  LEU ARG TYR HIS GLN VAL GLY GLN GLY PRO VAL LEU ILE
SEQRES   3 C  279  PHE ILE PRO GLY ALA ASN GLY THR GLY ASP ILE PHE LEU
SEQRES   4 C  279  PRO LEU ALA GLU GLN LEU LYS ASP HIS PHE THR VAL VAL
SEQRES   5 C  279  ALA VAL ASP ARG ARG ASP TYR GLY GLU SER GLU LEU THR
SEQRES   6 C  279  GLU PRO LEU PRO ASP SER ALA SER ASN PRO ASP SER ASP
SEQRES   7 C  279  TYR ARG VAL LYS ARG ASP ALA GLN ASP ILE ALA GLU LEU
SEQRES   8 C  279  ALA LYS SER LEU SER ASP GLU PRO VAL TYR ILE LEU GLY
SEQRES   9 C  279  SER SER SER GLY SER ILE VAL ALA MET HIS VAL LEU LYS
SEQRES  10 C  279  ASP TYR PRO GLU VAL VAL LYS LYS ILE ALA PHE HIS GLU
SEQRES  11 C  279  PRO PRO ILE ASN THR PHE LEU PRO ASP SER THR TYR TRP
SEQRES  12 C  279  LYS ASP LYS ASN ASP ASP ILE VAL HIS GLN ILE LEU THR
SEQRES  13 C  279  GLU GLY LEU GLU LYS GLY MET LYS THR PHE GLY GLU THR
SEQRES  14 C  279  LEU ASN ILE ALA PRO ILE ASP ALA LYS MET MET SER GLN
SEQRES  15 C  279  PRO ALA ASP THR GLU GLU GLY ARG ILE GLU GLN TYR LYS
SEQRES  16 C  279  ARG THR MET PHE TRP LEU GLU PHE GLU ILE ARG GLN TYR
SEQRES  17 C  279  THR HIS SER ASN ILE THR LEU ASP ASP PHE THR LYS TYR
SEQRES  18 C  279  SER ASP LYS ILE THR LEU LEU ASN GLY THR ASP SER ARG
SEQRES  19 C  279  GLY SER PHE PRO GLN ASP VAL ASN PHE TYR ILE ASN LYS
SEQRES  20 C  279  GLU THR GLY ILE PRO ILE VAL ASP ILE PRO GLY GLY HIS
SEQRES  21 C  279  LEU GLY TYR ILE GLN LYS PRO GLU GLY PHE ALA ASP VAL
SEQRES  22 C  279  LEU LEU ASN MET TRP GLY
SEQRES   1 D  279  GLY PRO GLY MET GLU THR LEU GLU LEU GLN GLY ALA LYS
SEQRES   2 D  279  LEU ARG TYR HIS GLN VAL GLY GLN GLY PRO VAL LEU ILE
SEQRES   3 D  279  PHE ILE PRO GLY ALA ASN GLY THR GLY ASP ILE PHE LEU
SEQRES   4 D  279  PRO LEU ALA GLU GLN LEU LYS ASP HIS PHE THR VAL VAL
SEQRES   5 D  279  ALA VAL ASP ARG ARG ASP TYR GLY GLU SER GLU LEU THR
SEQRES   6 D  279  GLU PRO LEU PRO ASP SER ALA SER ASN PRO ASP SER ASP
SEQRES   7 D  279  TYR ARG VAL LYS ARG ASP ALA GLN ASP ILE ALA GLU LEU
SEQRES   8 D  279  ALA LYS SER LEU SER ASP GLU PRO VAL TYR ILE LEU GLY
SEQRES   9 D  279  SER SER SER GLY SER ILE VAL ALA MET HIS VAL LEU LYS
SEQRES  10 D  279  ASP TYR PRO GLU VAL VAL LYS LYS ILE ALA PHE HIS GLU
SEQRES  11 D  279  PRO PRO ILE ASN THR PHE LEU PRO ASP SER THR TYR TRP
SEQRES  12 D  279  LYS ASP LYS ASN ASP ASP ILE VAL HIS GLN ILE LEU THR
SEQRES  13 D  279  GLU GLY LEU GLU LYS GLY MET LYS THR PHE GLY GLU THR
SEQRES  14 D  279  LEU ASN ILE ALA PRO ILE ASP ALA LYS MET MET SER GLN
SEQRES  15 D  279  PRO ALA ASP THR GLU GLU GLY ARG ILE GLU GLN TYR LYS
SEQRES  16 D  279  ARG THR MET PHE TRP LEU GLU PHE GLU ILE ARG GLN TYR
SEQRES  17 D  279  THR HIS SER ASN ILE THR LEU ASP ASP PHE THR LYS TYR
SEQRES  18 D  279  SER ASP LYS ILE THR LEU LEU ASN GLY THR ASP SER ARG
SEQRES  19 D  279  GLY SER PHE PRO GLN ASP VAL ASN PHE TYR ILE ASN LYS
SEQRES  20 D  279  GLU THR GLY ILE PRO ILE VAL ASP ILE PRO GLY GLY HIS
SEQRES  21 D  279  LEU GLY TYR ILE GLN LYS PRO GLU GLY PHE ALA ASP VAL
SEQRES  22 D  279  LEU LEU ASN MET TRP GLY
HET    XPU  A 301      13
HET    XPU  B 301      13
HET    XPU  C 301      13
HET    XPU  D 301      13
HETNAM     XPU 2-METHYL-4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-
HETNAM   2 XPU  YL)BENZENE-1-SULFONAMIDE
FORMUL   5  XPU    4(C13 H20 B N O4 S)
FORMUL   9  HOH   *76(H2 O)
HELIX    1 AA1 THR A   31  ILE A   34  5                                   4
HELIX    2 AA2 PHE A   35  LYS A   43  1                                   9
HELIX    3 AA3 PRO A   66  SER A   70  5                                   5
HELIX    4 AA4 ASP A   75  SER A   93  1                                  19
HELIX    5 AA5 SER A  103  TYR A  116  1                                  14
HELIX    6 AA6 ASP A  136  THR A  153  1                                  18
HELIX    7 AA7 THR A  153  LEU A  167  1                                  15
HELIX    8 AA8 ALA A  170  SER A  178  1                                   9
HELIX    9 AA9 THR A  183  GLU A  201  1                                  19
HELIX   10 AB1 GLU A  201  HIS A  207  1                                   7
HELIX   11 AB2 THR A  211  LYS A  217  1                                   7
HELIX   12 AB3 TYR A  218  ASP A  220  5                                   3
HELIX   13 AB4 SER A  233  GLY A  247  1                                  15
HELIX   14 AB5 LEU A  258  LYS A  263  1                                   6
HELIX   15 AB6 LYS A  263  GLY A  276  1                                  14
HELIX   16 AB7 THR B   31  ILE B   34  5                                   4
HELIX   17 AB8 PHE B   35  LYS B   43  1                                   9
HELIX   18 AB9 PRO B   66  ASN B   71  5                                   6
HELIX   19 AC1 ASP B   75  SER B   93  1                                  19
HELIX   20 AC2 SER B  103  TYR B  116  1                                  14
HELIX   21 AC3 ASP B  136  THR B  153  1                                  18
HELIX   22 AC4 THR B  153  LEU B  167  1                                  15
HELIX   23 AC5 ALA B  170  SER B  178  1                                   9
HELIX   24 AC6 THR B  183  GLU B  201  1                                  19
HELIX   25 AC7 GLU B  201  HIS B  207  1                                   7
HELIX   26 AC8 THR B  211  LYS B  217  1                                   7
HELIX   27 AC9 TYR B  218  ASP B  220  5                                   3
HELIX   28 AD1 SER B  233  GLY B  247  1                                  15
HELIX   29 AD2 LEU B  258  LYS B  263  1                                   6
HELIX   30 AD3 LYS B  263  GLY B  276  1                                  14
HELIX   31 AD4 THR C   31  ILE C   34  5                                   4
HELIX   32 AD5 PHE C   35  LYS C   43  1                                   9
HELIX   33 AD6 PRO C   66  ASN C   71  5                                   6
HELIX   34 AD7 ASP C   75  SER C   93  1                                  19
HELIX   35 AD8 SER C  103  TYR C  116  1                                  14
HELIX   36 AD9 ASP C  136  THR C  153  1                                  18
HELIX   37 AE1 THR C  153  LEU C  167  1                                  15
HELIX   38 AE2 ALA C  170  GLN C  179  1                                  10
HELIX   39 AE3 THR C  183  GLU C  201  1                                  19
HELIX   40 AE4 GLU C  201  HIS C  207  1                                   7
HELIX   41 AE5 LEU C  212  LYS C  217  1                                   6
HELIX   42 AE6 TYR C  218  ASP C  220  5                                   3
HELIX   43 AE7 SER C  233  GLY C  247  1                                  15
HELIX   44 AE8 LEU C  258  LYS C  263  1                                   6
HELIX   45 AE9 LYS C  263  GLY C  276  1                                  14
HELIX   46 AF1 THR D   31  ILE D   34  5                                   4
HELIX   47 AF2 PHE D   35  LYS D   43  1                                   9
HELIX   48 AF3 PRO D   66  ASN D   71  5                                   6
HELIX   49 AF4 ASP D   75  SER D   93  1                                  19
HELIX   50 AF5 SER D  103  TYR D  116  1                                  14
HELIX   51 AF6 ASP D  136  THR D  153  1                                  18
HELIX   52 AF7 THR D  153  LEU D  167  1                                  15
HELIX   53 AF8 ALA D  170  GLN D  179  1                                  10
HELIX   54 AF9 THR D  183  GLU D  201  1                                  19
HELIX   55 AG1 GLU D  201  HIS D  207  1                                   7
HELIX   56 AG2 LEU D  212  LYS D  217  1                                   6
HELIX   57 AG3 TYR D  218  ASP D  220  5                                   3
HELIX   58 AG4 SER D  233  GLY D  247  1                                  15
HELIX   59 AG5 LEU D  258  LYS D  263  1                                   6
HELIX   60 AG6 LYS D  263  GLY D  276  1                                  14
SHEET    1 AA1 3 GLU A   2  GLU A   5  0
SHEET    2 AA1 3 ALA A   9  VAL A  16 -1  O  LEU A  11   N  LEU A   4
SHEET    3 AA1 3 GLU A  60  LEU A  61 -1  O  GLU A  60   N  LYS A  10
SHEET    1 AA2 8 GLU A   2  GLU A   5  0
SHEET    2 AA2 8 ALA A   9  VAL A  16 -1  O  LEU A  11   N  LEU A   4
SHEET    3 AA2 8 THR A  47  ASP A  52 -1  O  ALA A  50   N  HIS A  14
SHEET    4 AA2 8 VAL A  21  ILE A  25  1  N  PHE A  24   O  VAL A  49
SHEET    5 AA2 8 VAL A  97  SER A 102  1  O  TYR A  98   N  ILE A  23
SHEET    6 AA2 8 VAL A 120  HIS A 126  1  O  HIS A 126   N  GLY A 101
SHEET    7 AA2 8 ILE B 222  GLY B 227  1  O  THR B 223   N  PHE A 125
SHEET    8 AA2 8 ILE B 250  ILE B 253  1  O  VAL B 251   N  LEU B 224
SHEET    1 AA3 8 ILE A 250  ILE A 253  0
SHEET    2 AA3 8 ILE A 222  GLY A 227  1  N  LEU A 224   O  VAL A 251
SHEET    3 AA3 8 VAL B 120  HIS B 126  1  O  PHE B 125   N  THR A 223
SHEET    4 AA3 8 VAL B  97  SER B 102  1  N  VAL B  97   O  LYS B 121
SHEET    5 AA3 8 VAL B  21  ILE B  25  1  N  ILE B  23   O  TYR B  98
SHEET    6 AA3 8 THR B  47  ASP B  52  1  O  VAL B  49   N  PHE B  24
SHEET    7 AA3 8 ALA B   9  GLY B  17 -1  N  HIS B  14   O  ALA B  50
SHEET    8 AA3 8 GLU B   2  LEU B   6 -1  N  LEU B   4   O  LEU B  11
SHEET    1 AA4 8 ILE A 250  ILE A 253  0
SHEET    2 AA4 8 ILE A 222  GLY A 227  1  N  LEU A 224   O  VAL A 251
SHEET    3 AA4 8 VAL B 120  HIS B 126  1  O  PHE B 125   N  THR A 223
SHEET    4 AA4 8 VAL B  97  SER B 102  1  N  VAL B  97   O  LYS B 121
SHEET    5 AA4 8 VAL B  21  ILE B  25  1  N  ILE B  23   O  TYR B  98
SHEET    6 AA4 8 THR B  47  ASP B  52  1  O  VAL B  49   N  PHE B  24
SHEET    7 AA4 8 ALA B   9  GLY B  17 -1  N  HIS B  14   O  ALA B  50
SHEET    8 AA4 8 GLU B  60  LEU B  61 -1  O  GLU B  60   N  LYS B  10
SHEET    1 AA5 3 GLU C   2  LEU C   6  0
SHEET    2 AA5 3 ALA C   9  GLY C  17 -1  O  LEU C  11   N  LEU C   4
SHEET    3 AA5 3 GLU C  60  LEU C  61 -1  O  GLU C  60   N  LYS C  10
SHEET    1 AA6 8 GLU C   2  LEU C   6  0
SHEET    2 AA6 8 ALA C   9  GLY C  17 -1  O  LEU C  11   N  LEU C   4
SHEET    3 AA6 8 THR C  47  ASP C  52 -1  O  ALA C  50   N  HIS C  14
SHEET    4 AA6 8 VAL C  21  ILE C  25  1  N  PHE C  24   O  VAL C  49
SHEET    5 AA6 8 VAL C  97  SER C 102  1  O  TYR C  98   N  ILE C  23
SHEET    6 AA6 8 VAL C 120  HIS C 126  1  O  LYS C 121   N  VAL C  97
SHEET    7 AA6 8 ILE D 222  GLY D 227  1  O  THR D 223   N  ILE C 123
SHEET    8 AA6 8 ILE D 250  ILE D 253  1  O  VAL D 251   N  LEU D 224
SHEET    1 AA7 8 ILE C 250  ILE C 253  0
SHEET    2 AA7 8 ILE C 222  GLY C 227  1  N  LEU C 224   O  VAL C 251
SHEET    3 AA7 8 VAL D 120  HIS D 126  1  O  PHE D 125   N  THR C 223
SHEET    4 AA7 8 VAL D  97  SER D 102  1  N  VAL D  97   O  LYS D 121
SHEET    5 AA7 8 VAL D  21  ILE D  25  1  N  ILE D  23   O  TYR D  98
SHEET    6 AA7 8 THR D  47  VAL D  51  1  O  VAL D  49   N  PHE D  24
SHEET    7 AA7 8 ALA D   9  VAL D  16 -1  N  HIS D  14   O  ALA D  50
SHEET    8 AA7 8 GLU D   2  LEU D   6 -1  N  LEU D   4   O  LEU D  11
LINK         OG  SER A 103                 B1  XPU A 301     1555   1555  1.39
LINK         NE2 HIS A 257                 B1  XPU B 301     1555   1555  1.44
LINK         B1  XPU A 301                 NE2 HIS B 257     1555   1555  1.44
LINK         OG  SER B 103                 B1  XPU B 301     1555   1555  1.40
LINK         OG  SER C 103                 B1  XPU C 301     1555   1555  1.40
LINK         NE2 HIS C 257                 B1  XPU D 301     1555   1555  1.43
LINK         B1  XPU C 301                 NE2 HIS D 257     1555   1555  1.44
LINK         OG  SER D 103                 B1  XPU D 301     1555   1555  1.40
CRYST1  212.286  212.286  212.286  90.00  90.00  90.00 I 2 3        96
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.004711  0.000000  0.000000        0.00000
SCALE2      0.000000  0.004711  0.000000        0.00000
SCALE3      0.000000  0.000000  0.004711        0.00000
TER    2201      GLY A 276
TER    4402      GLY B 276
TER    6603      GLY C 276
TER    8804      GLY D 276
MASTER      579    0    4   60   46    0    0    6 8928    4   60   88
END