longtext: 9HL5-pdb

content
HEADER    HYDROLASE                               04-DEC-24   9HL5
TITLE     CRYSTAL STRUCTURE OF HALO-TOLERANT PETASE FROM MARINE METAGENOME
TITLE    2 (HALOPETASE1)
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: ALPHA/BETA HYDROLASE;
COMPND   3 CHAIN: A;
COMPND   4 ENGINEERED: YES;
COMPND   5 OTHER_DETAILS: FIRST (1, S) AND LAST (270, A) AMINO ACIDS ARE FROM
COMPND   6 FX-CLONING.
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: UNCULTURED BACTERIUM;
SOURCE   3 ORGANISM_TAXID: 77133;
SOURCE   4 GENE: BXT89_15940;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    PETASE, HALO-TOLERANT, HYDROLASE, HALOPETASE, HALOPETASE1,
KEYWDS   2 POLYESTERASE, MARINE, METAGENOME
EXPDTA    X-RAY DIFFRACTION
AUTHOR    O.TURAK,M.KRIEGEL,B.HOCKER
REVDAT   1   01-OCT-25 9HL5    0
JRNL        AUTH   O.TURAK,A.GAGSTEIGER,A.UPADHYAY,M.KRIEGEL,P.SALEIN,
JRNL        AUTH 2 S.BOHNKE-BRANDT,S.AGARWAL,E.BORCHERT,B.HOCKER
JRNL        TITL   A THIRD TYPE OF PETASE FROM THE MARINE HALOPSEUDOMONAS
JRNL        TITL 2 LINEAGE.
JRNL        REF    PROTEIN SCI.                  V.  34 70305 2025
JRNL        REFN                   ESSN 1469-896X
JRNL        PMID   40960396
JRNL        DOI    10.1002/PRO.70305
REMARK   2
REMARK   2 RESOLUTION.    1.16 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.21.1-5286
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : GEOSTD + MONOMER LIBRARY + CDL V1.2
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.16
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 35.27
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.9
REMARK   3   NUMBER OF REFLECTIONS             : 93271
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.110
REMARK   3   R VALUE            (WORKING SET) : 0.110
REMARK   3   FREE R VALUE                     : 0.129
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 2.240
REMARK   3   FREE R VALUE TEST SET COUNT      : 2092
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 35.2700 -  2.8600    1.00     6412   147  0.1512 0.1664
REMARK   3     2  2.8600 -  2.2700    1.00     6177   142  0.1099 0.1162
REMARK   3     3  2.2700 -  1.9800    1.00     6138   141  0.0873 0.1017
REMARK   3     4  1.9800 -  1.8000    1.00     6087   140  0.0831 0.1099
REMARK   3     5  1.8000 -  1.6700    1.00     6093   140  0.0825 0.1077
REMARK   3     6  1.6700 -  1.5700    1.00     6072   139  0.0789 0.1011
REMARK   3     7  1.5700 -  1.5000    1.00     6047   139  0.0777 0.1041
REMARK   3     8  1.5000 -  1.4300    1.00     6076   140  0.0823 0.1085
REMARK   3     9  1.4300 -  1.3800    1.00     6007   137  0.0915 0.1238
REMARK   3    10  1.3800 -  1.3300    1.00     6040   138  0.0961 0.1090
REMARK   3    11  1.3300 -  1.2900    1.00     6034   139  0.1092 0.1374
REMARK   3    12  1.2900 -  1.2500    1.00     5984   136  0.1193 0.1608
REMARK   3    13  1.2500 -  1.2200    1.00     6003   138  0.1257 0.1490
REMARK   3    14  1.2200 -  1.1900    1.00     6015   139  0.1368 0.1571
REMARK   3    15  1.1900 -  1.1600    1.00     5994   137  0.1447 0.1510
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.10
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.069
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 10.190
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 8.77
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 13.38
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.007           2256
REMARK   3   ANGLE     :  1.059           3020
REMARK   3   CHIRALITY :  0.089            309
REMARK   3   PLANARITY :  0.011            388
REMARK   3   DIHEDRAL  : 14.576            832
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 9HL5 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 04-DEC-24.
REMARK 100 THE DEPOSITION ID IS D_1292143594.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 26-APR-24
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : PETRA III, EMBL C/O DESY
REMARK 200  BEAMLINE                       : P13 (MX1)
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9763
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER X 16M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : XDS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 93276
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.160
REMARK 200  RESOLUTION RANGE LOW       (A) : 35.270
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9
REMARK 200  DATA REDUNDANCY                : 6.600
REMARK 200  R MERGE                    (I) : 0.06117
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 15.8600
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.16
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.19
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.8
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 4.170
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 48.89
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.41
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M IMIDAZOLE, 40 % (V/V) PEG400,
REMARK 280  VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       26.17000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       36.23000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       35.26500
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       36.23000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       26.17000
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       35.26500
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 6840 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 10420 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: 56.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    HOH A   401     O    HOH A   548              1.94
REMARK 500   O    HOH A   418     O    HOH A   559              1.95
REMARK 500   O    HOH A   408     O    HOH A   558              1.96
REMARK 500   O    HOH A   563     O    HOH A   564              2.01
REMARK 500   O    HOH A   450     O    HOH A   469              2.03
REMARK 500   O    HOH A   466     O    HOH A   557              2.04
REMARK 500   O    HOH A   576     O    HOH A   583              2.04
REMARK 500   O3   GOL A   303     O    HOH A   401              2.05
REMARK 500   O    HOH A   530     O    HOH A   574              2.06
REMARK 500   O    HOH A   536     O    HOH A   571              2.08
REMARK 500   OG   SER A   239     O    HOH A   402              2.09
REMARK 500   O    HOH A   416     O    HOH A   561              2.11
REMARK 500   O    HOH A   534     O    HOH A   573              2.12
REMARK 500   O    HOH A   555     O    HOH A   569              2.15
REMARK 500   O    HOH A   482     O    HOH A   581              2.16
REMARK 500   O    HOH A   540     O    HOH A   541              2.16
REMARK 500   O    HOH A   547     O    HOH A   572              2.17
REMARK 500   OD2  ASP A   277     O    HOH A   403              2.17
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE
REMARK 500   O    HOH A   442     O    HOH A   481     2554     1.88
REMARK 500   O    HOH A   432     O    HOH A   489     4445     1.91
REMARK 500   O    HOH A   412     O    HOH A   502     2555     1.92
REMARK 500   O    HOH A   409     O    HOH A   559     2555     2.03
REMARK 500   O3   GOL A   320     O    HOH A   436     4545     2.15
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    LEU A  59      -45.49     70.39
REMARK 500    THR A  86       -7.40     74.94
REMARK 500    SER A 153     -131.11     67.86
REMARK 500    ALA A 206       70.76   -113.23
REMARK 500
REMARK 500 REMARK: NULL
DBREF  9HL5 A   20   287  PDB    9HL5     9HL5            20    287
SEQRES   1 A  268  SER THR ASN PRO GLY GLY GLY GLY GLY GLY SER ASN PRO
SEQRES   2 A  268  ASP THR GLY THR GLY PHE PRO GLY VAL SER SER PHE SER
SEQRES   3 A  268  ALA ASP GLY SER PHE ALA THR THR SER GLY SER ALA GLY
SEQRES   4 A  268  LEU SER CYS THR VAL PHE ARG PRO SER THR LEU GLY ALA
SEQRES   5 A  268  ASN GLY LEU LYS HIS PRO ILE ILE VAL TRP GLY ASN GLY
SEQRES   6 A  268  THR THR ALA SER PRO SER THR TYR SER GLY ILE LEU GLU
SEQRES   7 A  268  HIS TRP ALA SER HIS GLY PHE VAL VAL ILE ALA ALA ASN
SEQRES   8 A  268  THR SER ASN ALA GLY THR GLY GLN ASP MET LEU ASN CYS
SEQRES   9 A  268  VAL ASP TYR LEU THR THR GLN ASN ASN ARG SER THR GLY
SEQRES  10 A  268  THR TYR ALA ASN LYS LEU ASP LEU ASN ARG ILE GLY ALA
SEQRES  11 A  268  ALA GLY HIS SER GLN GLY GLY GLY GLY THR ILE MET ALA
SEQRES  12 A  268  GLY GLN ASP TYR ARG ILE LYS VAL THR ALA PRO PHE GLN
SEQRES  13 A  268  PRO TYR THR ILE GLY LEU GLY HIS ASN SER SER SER GLN
SEQRES  14 A  268  SER ASN GLN ASN GLY PRO MET PHE LEU MET THR GLY SER
SEQRES  15 A  268  ALA ASP THR ILE ALA SER PRO THR LEU ASN ALA LEU PRO
SEQRES  16 A  268  VAL TYR ASN ARG ALA ASN VAL PRO VAL PHE TRP GLY GLU
SEQRES  17 A  268  LEU SER GLY ALA SER HIS PHE GLU PRO VAL GLY SER ALA
SEQRES  18 A  268  GLY ASP PHE ARG GLY PRO SER THR ALA TRP PHE ARG TYR
SEQRES  19 A  268  HIS LEU MET ASP ASP ALA SER ALA GLU ASP THR PHE TYR
SEQRES  20 A  268  GLY SER ASN CYS ASP LEU CYS THR ASP ASN ASP TRP GLU
SEQRES  21 A  268  VAL ARG ARG LYS GLY ILE ASN ALA
HET    GOL  A 301      14
HET    GOL  A 302      14
HET    GOL  A 303      14
HET    GOL  A 304      14
HET    GOL  A 305      14
HET    PGE  A 306      24
HET    PG4  A 307      31
HET    PEG  A 308      17
HET    PEG  A 309      17
HET    PEG  A 310      17
HET    GOL  A 311      14
HET    EDO  A 312      10
HET    EDO  A 313      10
HET    GOL  A 314      14
HET    PGE  A 315      24
HET    EDO  A 316      10
HET    EDO  A 317      10
HET    GOL  A 318      14
HET    GOL  A 319      14
HET    GOL  A 320      14
HET    EDO  A 321      10
HET    GOL  A 322      14
HET    PGE  A 323      24
HET    EDO  A 324      10
HET    PGE  A 325      24
HET    EDO  A 326      10
HET    GOL  A 327      14
HET    GOL  A 328      14
HET    GOL  A 329      14
HET    PGE  A 330      24
HETNAM     GOL GLYCEROL
HETNAM     PGE TRIETHYLENE GLYCOL
HETNAM     PG4 TETRAETHYLENE GLYCOL
HETNAM     PEG DI(HYDROXYETHYL)ETHER
HETNAM     EDO 1,2-ETHANEDIOL
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL
HETSYN     EDO ETHYLENE GLYCOL
FORMUL   2  GOL    14(C3 H8 O3)
FORMUL   7  PGE    5(C6 H14 O4)
FORMUL   8  PG4    C8 H18 O5
FORMUL   9  PEG    3(C4 H10 O3)
FORMUL  13  EDO    7(C2 H6 O2)
FORMUL  32  HOH   *185(H2 O)
HELIX    1 AA1 GLY A   70  LEU A   74  5                                   5
HELIX    2 AA2 SER A   88  THR A   91  5                                   4
HELIX    3 AA3 TYR A   92  HIS A  102  1                                  11
HELIX    4 AA4 GLY A  117  ASN A  132  1                                  16
HELIX    5 AA5 SER A  153  GLY A  163  1                                  11
HELIX    6 AA6 ASN A  184  GLN A  191  5                                   8
HELIX    7 AA7 SER A  207  ALA A  212  1                                   6
HELIX    8 AA8 ALA A  212  ALA A  219  1                                   8
HELIX    9 AA9 ALA A  240  ASP A  242  5                                   3
HELIX   10 AB1 PHE A  243  ASP A  257  1                                  15
HELIX   11 AB2 ASP A  258  SER A  260  5                                   3
HELIX   12 AB3 ALA A  261  TYR A  266  1                                   6
HELIX   13 AB4 ASP A  271  ASP A  275  5                                   5
SHEET    1 AA1 6 THR A  52  ALA A  57  0
SHEET    2 AA1 6 CYS A  61  PRO A  66 -1  O  VAL A  63   N  GLY A  55
SHEET    3 AA1 6 VAL A 105  ALA A 109 -1  O  ALA A 108   N  THR A  62
SHEET    4 AA1 6 HIS A  76  GLY A  82  1  N  PRO A  77   O  VAL A 105
SHEET    5 AA1 6 LEU A 142  HIS A 152  1  O  ASP A 143   N  HIS A  76
SHEET    6 AA1 6 VAL A 170  PHE A 174  1  O  PHE A 174   N  GLY A 151
SHEET    1 AA2 3 MET A 195  GLY A 200  0
SHEET    2 AA2 3 VAL A 223  LEU A 228  1  O  PHE A 224   N  MET A 195
SHEET    3 AA2 3 TRP A 278  LYS A 283 -1  O  ARG A 281   N  TRP A 225
SSBOND   1 CYS A   61    CYS A  123                          1555   1555  2.06
SSBOND   2 CYS A  270    CYS A  273                          1555   1555  2.06
CRYST1   52.340   70.530   72.460  90.00  90.00  90.00 P 21 21 21    4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.019106  0.000000  0.000000        0.00000
SCALE2      0.000000  0.014178  0.000000        0.00000
SCALE3      0.000000  0.000000  0.013801        0.00000
TER    3924      ALA A 287
MASTER      277    0   30   13    9    0    0    6 2335    1  472   21
END