longtext: 9HYD-pdb

content
HEADER    HYDROLASE                               09-JAN-25   9HYD
TITLE     PETASESM14 FROM MARINE-SPONGE STREPTOMYCES SP.
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: CUTINASE;
COMPND   3 CHAIN: A;
COMPND   4 EC: 3.1.1.74;
COMPND   5 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: STREPTOMYCES SP. SM14;
SOURCE   3 ORGANISM_TAXID: 1736045;
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   5 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS    PETASE, POLYETHYLENE TEREPHTHALATE, SALT TOLERANCE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    S.BHATTACHARYA,R.CASTAGNA,E.PARISINI
REVDAT   1   19-NOV-25 9HYD    0
JRNL        AUTH   A.CARLETTI,S.BHATTACHARYA,S.PEDRONI,M.BERTO,R.BONETTINI,
JRNL        AUTH 2 R.CASTAGNA,E.PARISINI,G.DI ROCCO
JRNL        TITL   FUNCTIONAL AND STRUCTURAL CHARACTERIZATION OF PETASE SM14
JRNL        TITL 2 FROM MARINE-SPONGE STREPTOMYCES SP. ACTIVE ON POLYETHYLENE
JRNL        TITL 3 TEREPHTHALATE.
JRNL        REF    ACS SUSTAIN CHEM ENG          V.  13  7460 2025
JRNL        REFN                   ESSN 2168-0485
JRNL        PMID   40443410
JRNL        DOI    10.1021/ACSSUSCHEMENG.5C00737
REMARK   2
REMARK   2 RESOLUTION.    1.43 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.8.0352
REMARK   3   AUTHORS     : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK   3               : NICHOLLS,WINN,LONG,VAGIN
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.43
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 83.99
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.0
REMARK   3   NUMBER OF REFLECTIONS             : 45061
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : NONE
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL
REMARK   3   R VALUE     (WORKING + TEST SET) : NULL
REMARK   3   R VALUE            (WORKING SET) : 0.136
REMARK   3   FREE R VALUE                     : 0.202
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.058
REMARK   3   FREE R VALUE TEST SET COUNT      : 2279
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : 20
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.43
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.46
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 3152
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 100.0
REMARK   3   BIN R VALUE           (WORKING SET) : 0.3550
REMARK   3   BIN FREE R VALUE SET COUNT          : 160
REMARK   3   BIN FREE R VALUE                    : 0.3560
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 1994
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 30
REMARK   3   SOLVENT ATOMS            : 293
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 22.79
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 0.16700
REMARK   3    B22 (A**2) : 0.16700
REMARK   3    B33 (A**2) : -0.54300
REMARK   3    B12 (A**2) : 0.08400
REMARK   3    B13 (A**2) : 0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.065
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.069
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.076
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 4.998
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.986
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.968
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  2097 ; 0.009 ; 0.012
REMARK   3   BOND LENGTHS OTHERS               (A):  1832 ; 0.004 ; 0.016
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  2851 ; 1.623 ; 1.652
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  4275 ; 0.575 ; 1.559
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   268 ; 6.789 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):    23 ;10.196 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   313 ;13.218 ;10.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   307 ; 0.079 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  2493 ; 0.009 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):   439 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):   400 ; 0.232 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):    66 ; 0.221 ; 0.200
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  1031 ; 0.180 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   239 ; 0.211 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  1054 ; 3.051 ; 2.341
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  1053 ; 3.043 ; 2.340
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  1322 ; 3.296 ; 3.515
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  1323 ; 3.295 ; 3.516
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  1043 ; 5.697 ; 2.652
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  1044 ; 5.695 ; 2.653
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1527 ; 5.827 ; 3.862
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  1528 ; 5.825 ; 3.863
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  3929 ; 5.932 ; 3.000
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK BULK SOLVENT
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THEIR
REMARK   3  RIDING POSITIONS
REMARK   4
REMARK   4 9HYD COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 09-JAN-25.
REMARK 100 THE DEPOSITION ID IS D_1292144466.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 30-JAN-24
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : DIAMOND
REMARK 200  BEAMLINE                       : I03
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.7133
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER2 XE 16M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : AUTOPROC
REMARK 200  DATA SCALING SOFTWARE          : AUTOPROC
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 45062
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.425
REMARK 200  RESOLUTION RANGE LOW       (A) : 83.990
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0
REMARK 200  DATA REDUNDANCY                : 21.30
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 8.9000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.43
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.45
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: REFMAC
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 41.63
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.11
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M AMMONIUM SULFATE, 0.1 M MES
REMARK 280  MONOHYDRATE PH 6.5, 30% W/V POLYETHYLENE GLYCOL MONOMETHYL ETHER
REMARK 280  5,000, VAPOR DIFFUSION, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 61
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -Y,X-Y,Z+1/3
REMARK 290       3555   -X+Y,-X,Z+2/3
REMARK 290       4555   -X,-Y,Z+1/2
REMARK 290       5555   Y,-X+Y,Z+5/6
REMARK 290       6555   X-Y,X,Z+1/6
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       14.93833
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       29.87667
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       22.40750
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       37.34583
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000        7.46917
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     GLY A    21
REMARK 465     TRP A    22
REMARK 465     GLU A    23
REMARK 465     LEU A    24
REMARK 465     GLN A    25
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   H    GLY A    46     HG   SER A   104              1.10
REMARK 500   HG1  THR A   114     H    ASP A   115              1.25
REMARK 500   O    HOH A   476     O    HOH A   650              1.87
REMARK 500   O    HOH A   510     O    HOH A   628              1.88
REMARK 500   O    HOH A   473     O    HOH A   584              1.93
REMARK 500   O    HOH A   607     O    HOH A   688              2.05
REMARK 500   O    HOH A   453     O    HOH A   459              2.14
REMARK 500   O    HOH A   645     O    HOH A   676              2.15
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    ARG A 271   CZ    ARG A 271   NH2     0.101
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ARG A 271   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.1 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ALA A  90      -19.53     89.39
REMARK 500    SER A 156     -127.42     69.72
REMARK 500    THR A 179       60.47     28.03
REMARK 500    HIS A 210      -86.65   -119.04
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        RMS     TYPE
REMARK 500    ARG A  78         0.11    SIDE CHAIN
REMARK 500    ARG A 173         0.14    SIDE CHAIN
REMARK 500    ARG A 208         0.10    SIDE CHAIN
REMARK 500
REMARK 500 REMARK: NULL
DBREF1 9HYD A   26   284  UNP                  A0A679PDB4_9ACTN
DBREF2 9HYD A     A0A679PDB4                         26         284
SEQADV 9HYD GLY A   21  UNP  A0A679PDB           EXPRESSION TAG
SEQADV 9HYD TRP A   22  UNP  A0A679PDB           EXPRESSION TAG
SEQADV 9HYD GLU A   23  UNP  A0A679PDB           EXPRESSION TAG
SEQADV 9HYD LEU A   24  UNP  A0A679PDB           EXPRESSION TAG
SEQADV 9HYD GLN A   25  UNP  A0A679PDB           EXPRESSION TAG
SEQRES   1 A  264  GLY TRP GLU LEU GLN ALA GLN ASN PRO HIS GLU ARG GLY
SEQRES   2 A  264  PRO ASP PRO SER ASN SER TYR ILE GLU GLN ALA ARG GLY
SEQRES   3 A  264  SER TYR SER VAL SER GLN ARG SER ILE SER ARG LEU GLY
SEQRES   4 A  264  SER ASP GLY PHE ARG ASP GLY THR MET TYR TYR PRO THR
SEQRES   5 A  264  SER THR ALA ASP GLY ARG PHE GLY VAL VAL ALA ILE SER
SEQRES   6 A  264  PRO GLY TYR THR ALA SER GLU SER THR ILE ALA TRP LEU
SEQRES   7 A  264  GLY PRO ARG LEU ALA SER PHE GLY PHE VAL VAL VAL THR
SEQRES   8 A  264  ILE ASN THR ASP SER ARG TYR ASP GLN PRO ARG GLN ARG
SEQRES   9 A  264  ALA THR GLN LEU HIS ALA ALA LEU ASP HIS ALA ILE GLY
SEQRES  10 A  264  ASP SER VAL VAL GLY PRO ARG ILE ASP THR SER ARG GLN
SEQRES  11 A  264  ALA VAL MET GLY HIS SER MET GLY GLY GLY GLY ALA LEU
SEQRES  12 A  264  GLN ALA ALA GLU GLU ARG ASP GLU ILE ARG ALA ALA VAL
SEQRES  13 A  264  PRO LEU THR PRO TRP ASN LEU LYS LYS GLY TRP SER GLY
SEQRES  14 A  264  VAL ASP ALA ALA THR LEU VAL ILE GLY ALA GLU ASN ASP
SEQRES  15 A  264  ALA ILE ALA PRO VAL ARG SER HIS SER ILE PRO PHE TYR
SEQRES  16 A  264  GLU SER LEU THR ASN ALA GLU ARG ARG ALA TYR LEU GLU
SEQRES  17 A  264  LEU ARG ARG GLU GLY HIS PHE ALA PRO ASN SER SER ASN
SEQRES  18 A  264  THR LEU ILE ALA LYS TYR SER VAL SER TRP LEU LYS ARG
SEQRES  19 A  264  TYR VAL ASP ASN ASP LEU ARG TYR ASP GLN PHE ILE ASP
SEQRES  20 A  264  PRO GLY PRO ARG THR GLY ILE THR THR GLY VAL SER ASP
SEQRES  21 A  264  TYR ARG LEU GLY
HET    GOL  A 301      14
HET    GOL  A 302      14
HET    GOL  A 303      14
HET    GOL  A 304      14
HET    GOL  A 305      14
HETNAM     GOL GLYCEROL
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL
FORMUL   2  GOL    5(C3 H8 O3)
FORMUL   7  HOH   *293(H2 O)
HELIX    1 AA1 SER A   37  GLU A   42  1                                   6
HELIX    2 AA2 SER A   91  ALA A   96  5                                   6
HELIX    3 AA3 TRP A   97  SER A  104  1                                   8
HELIX    4 AA4 ASP A  115  ASP A  119  5                                   5
HELIX    5 AA5 GLN A  120  GLY A  137  1                                  18
HELIX    6 AA6 VAL A  141  PRO A  143  5                                   3
HELIX    7 AA7 SER A  156  ARG A  169  1                                  14
HELIX    8 AA8 HIS A  210  LEU A  218  1                                   9
HELIX    9 AA9 PHE A  235  SER A  239  5                                   5
HELIX   10 AB1 ASN A  241  ASP A  257  1                                  17
HELIX   11 AB2 ASP A  259  ARG A  261  5                                   3
HELIX   12 AB3 TYR A  262  ASP A  267  1                                   6
SHEET    1 AA1 9 VAL A  50  SER A  56  0
SHEET    2 AA1 9 ASP A  65  PRO A  71 -1  O  TYR A  70   N  SER A  51
SHEET    3 AA1 9 VAL A 108  ASN A 113 -1  O  VAL A 109   N  TYR A  69
SHEET    4 AA1 9 PHE A  79  SER A  85  1  N  VAL A  82   O  VAL A 108
SHEET    5 AA1 9 ILE A 145  HIS A 155  1  O  ASP A 146   N  PHE A  79
SHEET    6 AA1 9 ILE A 172  LEU A 178  1  O  LEU A 178   N  GLY A 154
SHEET    7 AA1 9 ALA A 193  ALA A 199  1  O  ILE A 197   N  PRO A 177
SHEET    8 AA1 9 ARG A 224  LEU A 229  1  O  LEU A 229   N  GLY A 198
SHEET    9 AA1 9 VAL A 278  GLY A 284 -1  O  GLY A 284   N  ARG A 224
CISPEP   1 ASP A  267    PRO A  268          0        12.59
CRYST1   96.983   96.983   44.815  90.00  90.00 120.00 P 61          6
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.010311  0.005953  0.000000        0.00000
SCALE2      0.000000  0.011906  0.000000        0.00000
SCALE3      0.000000  0.000000  0.022314        0.00000
TER    3973      GLY A 284
MASTER      349    0    5   12    9    0    0    6 2317    1   70   21
END