longtext: 9I50-pdb

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HEADER    HYDROLASE                               27-JAN-25   9I50
TITLE     CRYSTAL STRUCTURE OF FERULOYL ESTERASE FROM FUSARIUM OXYSPORUM G122S
TITLE    2 VARIANT
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: CARBOXYLIC ESTER HYDROLASE;
COMPND   3 CHAIN: A, B;
COMPND   4 EC: 3.1.1.-;
COMPND   5 ENGINEERED: YES;
COMPND   6 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: FUSARIUM OXYSPORUM;
SOURCE   3 ORGANISM_TAXID: 5507;
SOURCE   4 GENE: FAEC;
SOURCE   5 EXPRESSION_SYSTEM: KOMAGATAELLA PASTORIS;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 4922
KEYWDS    PLASTIC DEGRADING ENZYME, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    P.KARAMPA,M.DIMAROGONA,E.TOPAKAS,K.MAKRYNIOTIS,E.NIKOLAIVITS
REVDAT   1   01-APR-26 9I50    0
JRNL        AUTH   P.KARAMPA,K.MAKRYNIOTIS,T.I.SOUSANI,E.TOPAKAS,V.DASKALAKIS,
JRNL        AUTH 2 M.DIMAROGONA
JRNL        TITL   STRUCTURAL INSIGHTS INTO AN ENGINEERED FERULOYL ESTERASE
JRNL        TITL 2 WITH IMPROVED MHET DEGRADING PROPERTIES.
JRNL        REF    FEBS LETT.                                 2026
JRNL        REFN                   ISSN 0014-5793
JRNL        PMID   41797379
JRNL        DOI    10.1002/1873-3468.70322
REMARK   2
REMARK   2 RESOLUTION.    1.90 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.8.0419
REMARK   3   AUTHORS     : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK   3               : NICHOLLS,WINN,LONG,VAGIN
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.90
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 114.05
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.1
REMARK   3   NUMBER OF REFLECTIONS             : 107314
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : FREE R-VALUE
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL
REMARK   3   R VALUE     (WORKING + TEST SET) : NULL
REMARK   3   R VALUE            (WORKING SET) : 0.162
REMARK   3   FREE R VALUE                     : 0.197
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.067
REMARK   3   FREE R VALUE TEST SET COUNT      : 5438
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.90
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.95
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 7532
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 99.51
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2810
REMARK   3   BIN FREE R VALUE SET COUNT          : 388
REMARK   3   BIN FREE R VALUE                    : 0.2870
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 7914
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 614
REMARK   3   SOLVENT ATOMS            : 606
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 41.76
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 1.49000
REMARK   3    B22 (A**2) : -3.10200
REMARK   3    B33 (A**2) : 0.00600
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 3.86900
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.118
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.115
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.102
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 7.286
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.974
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.961
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  8857 ; 0.007 ; 0.012
REMARK   3   BOND LENGTHS OTHERS               (A):  7896 ; 0.001 ; 0.016
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 12067 ; 1.582 ; 1.883
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 18343 ; 0.559 ; 1.808
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  1032 ; 6.830 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):    44 ; 9.857 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1273 ;13.125 ;10.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):  NULL ;  NULL ;  NULL
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1386 ; 0.154 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A): 10109 ; 0.006 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):  2026 ; 0.002 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  1724 ; 0.241 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):    45 ; 0.215 ; 0.200
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  4432 ; 0.178 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   524 ; 0.140 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):     5 ; 0.142 ; 0.200
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  4107 ; 2.169 ; 3.356
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  4107 ; 2.168 ; 3.356
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  5146 ; 2.948 ; 6.027
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  5147 ; 2.949 ; 6.028
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  4750 ; 3.200 ; 3.817
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  4751 ; 3.200 ; 3.818
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  6921 ; 4.764 ; 6.815
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  6922 ; 4.764 ; 6.815
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 2
REMARK   3
REMARK   3   TLS GROUP : 1
REMARK   3    NUMBER OF COMPONENTS GROUP : 1
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    RESIDUE RANGE :   A    36        A   577
REMARK   3    ORIGIN FOR THE GROUP (A): -27.0722 -12.2206 -13.4412
REMARK   3    T TENSOR
REMARK   3      T11:   0.0420 T22:   0.0253
REMARK   3      T33:   0.0275 T12:   0.0123
REMARK   3      T13:   0.0156 T23:   0.0222
REMARK   3    L TENSOR
REMARK   3      L11:   0.8895 L22:   0.6762
REMARK   3      L33:   0.9405 L12:   0.2580
REMARK   3      L13:  -0.3944 L23:  -0.1992
REMARK   3    S TENSOR
REMARK   3      S11:   0.0136 S12:  -0.0443 S13:  -0.0527
REMARK   3      S21:   0.0940 S22:   0.0279 S23:   0.0824
REMARK   3      S31:   0.0827 S32:  -0.0471 S33:  -0.0415
REMARK   3
REMARK   3   TLS GROUP : 2
REMARK   3    NUMBER OF COMPONENTS GROUP : 0
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI
REMARK   3    ORIGIN FOR THE GROUP (A):   5.6687  10.8356 -41.9311
REMARK   3    T TENSOR
REMARK   3      T11:   0.0160 T22:   0.0152
REMARK   3      T33:   0.0196 T12:   0.0039
REMARK   3      T13:   0.0039 T23:   0.0092
REMARK   3    L TENSOR
REMARK   3      L11:   0.8445 L22:   0.7932
REMARK   3      L33:   0.8047 L12:   0.3659
REMARK   3      L13:  -0.4262 L23:  -0.3463
REMARK   3    S TENSOR
REMARK   3      S11:   0.0168 S12:  -0.0209 S13:   0.0565
REMARK   3      S21:  -0.0012 S22:  -0.0331 S23:  -0.0629
REMARK   3      S31:  -0.0759 S32:   0.0537 S33:   0.0163
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK BULK SOLVENT
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THEIR
REMARK   3  RIDING POSITIONS
REMARK   4
REMARK   4 9I50 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 27-JAN-25.
REMARK 100 THE DEPOSITION ID IS D_1292144891.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 08-DEC-23
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : PETRA III, EMBL C/O DESY
REMARK 200  BEAMLINE                       : P13 (MX1)
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9763
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER X 16M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS 8.0.016
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 107339
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.900
REMARK 200  RESOLUTION RANGE LOW       (A) : 114.050
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.2
REMARK 200  DATA REDUNDANCY                : 7.000
REMARK 200  R MERGE                    (I) : 0.07700
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 13.0000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 10.41
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 114.0
REMARK 200  COMPLETENESS FOR SHELL     (%) : 98.8
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.30
REMARK 200  R MERGE FOR SHELL          (I) : 0.02900
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 41.70
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER 8.0.016
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 58.78
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.98
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 35% (V/V) PEG400, 0.1M TRIS-HCL PH
REMARK 280  8.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293.15K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       44.63050
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, C, D, E
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B, F, G
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     SER A     1
REMARK 465     MET A     2
REMARK 465     ASP A     3
REMARK 465     THR A     4
REMARK 465     SER A     5
REMARK 465     THR A     6
REMARK 465     ASP A     7
REMARK 465     ILE A     8
REMARK 465     CYS A     9
REMARK 465     LEU A    10
REMARK 465     PRO A    11
REMARK 465     GLN A    12
REMARK 465     ASP A    13
REMARK 465     ASN A    14
REMARK 465     MET A    15
REMARK 465     ARG A    16
REMARK 465     PRO A    17
REMARK 465     THR A    18
REMARK 465     PHE A    19
REMARK 465     LEU A    20
REMARK 465     LEU A    21
REMARK 465     PHE A    22
REMARK 465     SER A    23
REMARK 465     GLY A    24
REMARK 465     LEU A    25
REMARK 465     GLY A    26
REMARK 465     ALA A    27
REMARK 465     CYS A    28
REMARK 465     ALA A    29
REMARK 465     ALA A    30
REMARK 465     ALA A    31
REMARK 465     GLY A    32
REMARK 465     LYS A    33
REMARK 465     GLY A    34
REMARK 465     ASP A    35
REMARK 465     GLN A   544
REMARK 465     LYS A   545
REMARK 465     LEU A   546
REMARK 465     ILE A   547
REMARK 465     SER A   548
REMARK 465     GLU A   549
REMARK 465     GLU A   550
REMARK 465     ASP A   551
REMARK 465     LEU A   552
REMARK 465     ASN A   553
REMARK 465     SER A   554
REMARK 465     ALA A   555
REMARK 465     VAL A   556
REMARK 465     ASP A   557
REMARK 465     HIS A   558
REMARK 465     HIS A   559
REMARK 465     HIS A   560
REMARK 465     HIS A   561
REMARK 465     HIS A   562
REMARK 465     HIS A   563
REMARK 465     SER B     1
REMARK 465     MET B     2
REMARK 465     ASP B     3
REMARK 465     THR B     4
REMARK 465     SER B     5
REMARK 465     THR B     6
REMARK 465     ASP B     7
REMARK 465     ILE B     8
REMARK 465     CYS B     9
REMARK 465     LEU B    10
REMARK 465     PRO B    11
REMARK 465     GLN B    12
REMARK 465     ASP B    13
REMARK 465     ASN B    14
REMARK 465     MET B    15
REMARK 465     ARG B    16
REMARK 465     PRO B    17
REMARK 465     THR B    18
REMARK 465     PHE B    19
REMARK 465     LEU B    20
REMARK 465     LEU B    21
REMARK 465     PHE B    22
REMARK 465     SER B    23
REMARK 465     GLY B    24
REMARK 465     LEU B    25
REMARK 465     GLY B    26
REMARK 465     ALA B    27
REMARK 465     CYS B    28
REMARK 465     ALA B    29
REMARK 465     ALA B    30
REMARK 465     ALA B    31
REMARK 465     GLY B    32
REMARK 465     LYS B    33
REMARK 465     GLY B    34
REMARK 465     ASP B    35
REMARK 465     GLN B   544
REMARK 465     LYS B   545
REMARK 465     LEU B   546
REMARK 465     ILE B   547
REMARK 465     SER B   548
REMARK 465     GLU B   549
REMARK 465     GLU B   550
REMARK 465     ASP B   551
REMARK 465     LEU B   552
REMARK 465     ASN B   553
REMARK 465     SER B   554
REMARK 465     ALA B   555
REMARK 465     VAL B   556
REMARK 465     ASP B   557
REMARK 465     HIS B   558
REMARK 465     HIS B   559
REMARK 465     HIS B   560
REMARK 465     HIS B   561
REMARK 465     HIS B   562
REMARK 465     HIS B   563
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500  HH21  ARG B   513     O    HOH B   708              1.59
REMARK 500   HO3  MAN G     8     O    HOH B   759              1.59
REMARK 500   ND2  ASN B   362     C1   NAG G     1              1.72
REMARK 500   ND2  ASN A   151     C1   NAG D     1              1.74
REMARK 500   ND2  ASN B   151     C1   NAG F     1              1.75
REMARK 500   ND2  ASN A    66     C1   NAG C     1              1.76
REMARK 500   ND2  ASN B   101     C1   NAG B   601              1.80
REMARK 500   ND2  ASN A   362     C1   NAG E     1              1.82
REMARK 500   ND2  ASN A   101     C1   NAG A   601              1.85
REMARK 500   ND2  ASN B    66     C1   NAG B   609              2.06
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    ARG A 229   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES
REMARK 500    ARG A 229   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.9 DEGREES
REMARK 500    ARG A 290   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.0 DEGREES
REMARK 500    ARG B 229   NE  -  CZ  -  NH2 ANGL. DEV. =  -5.1 DEGREES
REMARK 500    ARG B 513   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.1 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASN A 111       79.98   -100.05
REMARK 500    TYR A 170      -33.24   -152.86
REMARK 500    SER A 201     -128.31     77.23
REMARK 500    SER A 201     -128.31     84.67
REMARK 500    ALA A 225       55.39     35.54
REMARK 500    ALA A 227       42.28    -73.57
REMARK 500    ASP A 270      -94.43    -81.31
REMARK 500    LYS A 298      109.12     40.29
REMARK 500    LYS A 302      -92.88    -83.75
REMARK 500    ASN A 303       70.61   -107.05
REMARK 500    ASN A 383       62.58     38.37
REMARK 500    PHE A 441      -13.06   -142.95
REMARK 500    CYS A 453      -22.91     78.22
REMARK 500    LYS A 527      -48.50   -145.14
REMARK 500    TYR B 170      -29.06   -148.78
REMARK 500    SER B 201     -126.16     83.08
REMARK 500    SER B 201     -126.16     88.99
REMARK 500    ALA B 225       53.86     39.19
REMARK 500    ALA B 227       39.44    -73.24
REMARK 500    ASP B 270      -93.31    -86.24
REMARK 500    ASP B 439      -18.88    -48.98
REMARK 500    PHE B 441      -11.59   -146.66
REMARK 500    CYS B 453      -29.45     73.00
REMARK 500    LYS B 527      -57.73   -121.17
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS
REMARK 500
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.
REMARK 500                                 MODEL     OMEGA
REMARK 500 ALA A  125     GLY A  126                  135.25
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        RMS     TYPE
REMARK 500    ARG A 229         0.15    SIDE CHAIN
REMARK 500    ARG A 290         0.09    SIDE CHAIN
REMARK 500    ARG B 229         0.11    SIDE CHAIN
REMARK 500    ARG B 513         0.12    SIDE CHAIN
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A 982        DISTANCE =  5.94 ANGSTROMS
REMARK 525    HOH A 984        DISTANCE =  6.32 ANGSTROMS
REMARK 525    HOH A 985        DISTANCE =  7.15 ANGSTROMS
REMARK 525    HOH A 988        DISTANCE =  7.44 ANGSTROMS
REMARK 525    HOH B1016        DISTANCE =  6.10 ANGSTROMS
REMARK 525    HOH B1018        DISTANCE =  6.94 ANGSTROMS
REMARK 610
REMARK 610 MISSING HETEROATOM
REMARK 610 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 610 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 610 I=INSERTION CODE):
REMARK 610   M RES C SSEQI
REMARK 610     NAG C    1
REMARK 610     NAG D    1
REMARK 610     NAG E    1
REMARK 610     NAG F    1
REMARK 610     NAG G    1
REMARK 610     NAG A  601
REMARK 610     NAG A  608
REMARK 610     NAG B  601
REMARK 610     NAG B  608
REMARK 610     NAG B  609
REMARK 620
REMARK 620 METAL COORDINATION
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 620
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL
REMARK 620                              CA A 609  CA
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 ASP A 270   OD1
REMARK 620 2 ASP A 274   OD1 104.2
REMARK 620 3 ASP A 274   OD2  96.9  52.8
REMARK 620 4 VAL A 276   O    82.2  75.5 126.5
REMARK 620 5 ASP A 278   OD1  92.4 143.7 157.4  75.1
REMARK 620 6 ILE A 280   O    76.9 131.0  78.3 149.6  83.8
REMARK 620 7 HOH A 845   O   163.7  89.7  84.7 109.9  80.8  87.6
REMARK 620 N                    1     2     3     4     5     6
REMARK 620
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL
REMARK 620                              CA B 610  CA
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 ASP B 270   OD1
REMARK 620 2 ASP B 274   OD1 106.9
REMARK 620 3 ASP B 274   OD2  98.9  51.4
REMARK 620 4 VAL B 276   O    81.6  77.2 126.7
REMARK 620 5 ASP B 278   OD1  93.2 146.0 152.9  78.9
REMARK 620 6 ILE B 280   O    79.0 130.1  78.8 150.3  79.8
REMARK 620 7 HOH B 765   O   162.9  89.5  87.4 107.4  74.9  86.7
REMARK 620 N                    1     2     3     4     5     6
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 6FAT   RELATED DB: PDB
REMARK 900 RELATED ID: 8BHH   RELATED DB: PDB
DBREF1 9I50 A    1   563  UNP                  A0A1D3S5H0_FUSOX
DBREF2 9I50 A     A0A1D3S5H0                          1         563
DBREF1 9I50 B    1   563  UNP                  A0A1D3S5H0_FUSOX
DBREF2 9I50 B     A0A1D3S5H0                          1         563
SEQADV 9I50 SER A  122  UNP  A0A1D3S5H GLY   122 ENGINEERED MUTATION
SEQADV 9I50 SER B  122  UNP  A0A1D3S5H GLY   122 ENGINEERED MUTATION
SEQRES   1 A  563  SER MET ASP THR SER THR ASP ILE CYS LEU PRO GLN ASP
SEQRES   2 A  563  ASN MET ARG PRO THR PHE LEU LEU PHE SER GLY LEU GLY
SEQRES   3 A  563  ALA CYS ALA ALA ALA GLY LYS GLY ASP ASP PHE ALA ALA
SEQRES   4 A  563  LYS CYS ALA GLY PHE LYS THR SER LEU LYS LEU PRO ASN
SEQRES   5 A  563  THR LYS VAL TRP PHE THR GLU HIS VAL PRO ALA GLY LYS
SEQRES   6 A  563  ASN ILE THR PHE PRO ASP ASN HIS PRO THR CYS THR PRO
SEQRES   7 A  563  LYS SER THR ILE THR ASP VAL GLU ILE CYS ARG VAL ALA
SEQRES   8 A  563  MET PHE VAL THR THR GLY PRO LYS SER ASN LEU THR LEU
SEQRES   9 A  563  GLU ALA TRP LEU PRO SER ASN TRP THR GLY ARG PHE LEU
SEQRES  10 A  563  SER THR GLY ASN SER GLY MET ALA GLY CYS ILE GLN TYR
SEQRES  11 A  563  ASP ASP VAL ALA TYR GLY ALA GLY PHE GLY PHE ALA THR
SEQRES  12 A  563  VAL GLY ALA ASN ASN GLY HIS ASN GLY THR SER ALA VAL
SEQRES  13 A  563  SER MET TYR LYS ASN SER GLY VAL VAL GLU ASP TYR VAL
SEQRES  14 A  563  TYR ARG SER VAL HIS THR GLY THR VAL LEU GLY LYS GLU
SEQRES  15 A  563  LEU THR LYS LYS PHE TYR GLY LYS LYS HIS THR LYS SER
SEQRES  16 A  563  TYR TYR LEU GLY CYS SER THR GLY GLY ARG GLN GLY TRP
SEQRES  17 A  563  LYS GLU ALA GLN SER PHE PRO ASP ASP PHE ASP GLY ILE
SEQRES  18 A  563  VAL ALA GLY ALA PRO ALA MET ARG PHE ASN GLY LEU GLN
SEQRES  19 A  563  SER ARG SER GLY SER PHE TRP GLY ILE THR GLY PRO PRO
SEQRES  20 A  563  GLY ALA PRO THR HIS LEU SER PRO GLU GLU TRP ALA MET
SEQRES  21 A  563  VAL GLN LYS ASN VAL LEU VAL GLN CYS ASP GLU PRO LEU
SEQRES  22 A  563  ASP GLY VAL ALA ASP GLY ILE LEU GLU ASP PRO ASN LEU
SEQRES  23 A  563  CYS GLN TYR ARG PRO GLU ALA LEU VAL CYS SER LYS GLY
SEQRES  24 A  563  GLN THR LYS ASN CYS LEU THR GLY PRO GLN ILE GLU THR
SEQRES  25 A  563  VAL ARG LYS VAL PHE GLY PRO LEU TYR GLY ASN ASN GLY
SEQRES  26 A  563  THR TYR ILE TYR PRO ARG ILE PRO PRO GLY ALA ASP GLN
SEQRES  27 A  563  GLY PHE GLY PHE ALA ILE GLY GLU GLN PRO PHE PRO TYR
SEQRES  28 A  563  SER THR GLU TRP PHE GLN TYR VAL ILE TRP ASN ASP THR
SEQRES  29 A  563  LYS TRP ASP PRO ASN THR ILE GLY PRO ASN ASP TYR GLN
SEQRES  30 A  563  LYS ALA SER GLU VAL ASN PRO PHE ASN VAL GLU THR TRP
SEQRES  31 A  563  GLU GLY ASP LEU SER LYS PHE ARG LYS ARG GLY SER LYS
SEQRES  32 A  563  ILE ILE HIS TRP HIS GLY LEU GLU ASP GLY LEU ILE SER
SEQRES  33 A  563  SER ASP ASN SER MET GLU TYR TYR ASN HIS VAL SER ALA
SEQRES  34 A  563  THR MET GLY LEU SER ASN THR GLU LEU ASP GLU PHE TYR
SEQRES  35 A  563  ARG TYR PHE ARG VAL SER GLY CYS GLY HIS CYS SER GLY
SEQRES  36 A  563  GLY ILE GLY ALA ASN ARG ILE GLY ASN ASN ARG ALA ASN
SEQRES  37 A  563  LEU GLY GLY LYS GLU ALA LYS ASN ASN VAL LEU LEU ALA
SEQRES  38 A  563  LEU VAL LYS TRP VAL GLU GLU GLY GLN ALA PRO GLU THR
SEQRES  39 A  563  ILE THR GLY VAL ARG TYR VAL ASN GLY ALA THR THR GLY
SEQRES  40 A  563  LYS VAL GLU VAL GLU ARG ARG HIS CYS ARG TYR PRO TYR
SEQRES  41 A  563  ARG ASN VAL TRP ASP ARG LYS GLY ASN TYR LYS ASN PRO
SEQRES  42 A  563  ASP SER TRP LYS CYS GLU LEU PRO LEU GLU GLN LYS LEU
SEQRES  43 A  563  ILE SER GLU GLU ASP LEU ASN SER ALA VAL ASP HIS HIS
SEQRES  44 A  563  HIS HIS HIS HIS
SEQRES   1 B  563  SER MET ASP THR SER THR ASP ILE CYS LEU PRO GLN ASP
SEQRES   2 B  563  ASN MET ARG PRO THR PHE LEU LEU PHE SER GLY LEU GLY
SEQRES   3 B  563  ALA CYS ALA ALA ALA GLY LYS GLY ASP ASP PHE ALA ALA
SEQRES   4 B  563  LYS CYS ALA GLY PHE LYS THR SER LEU LYS LEU PRO ASN
SEQRES   5 B  563  THR LYS VAL TRP PHE THR GLU HIS VAL PRO ALA GLY LYS
SEQRES   6 B  563  ASN ILE THR PHE PRO ASP ASN HIS PRO THR CYS THR PRO
SEQRES   7 B  563  LYS SER THR ILE THR ASP VAL GLU ILE CYS ARG VAL ALA
SEQRES   8 B  563  MET PHE VAL THR THR GLY PRO LYS SER ASN LEU THR LEU
SEQRES   9 B  563  GLU ALA TRP LEU PRO SER ASN TRP THR GLY ARG PHE LEU
SEQRES  10 B  563  SER THR GLY ASN SER GLY MET ALA GLY CYS ILE GLN TYR
SEQRES  11 B  563  ASP ASP VAL ALA TYR GLY ALA GLY PHE GLY PHE ALA THR
SEQRES  12 B  563  VAL GLY ALA ASN ASN GLY HIS ASN GLY THR SER ALA VAL
SEQRES  13 B  563  SER MET TYR LYS ASN SER GLY VAL VAL GLU ASP TYR VAL
SEQRES  14 B  563  TYR ARG SER VAL HIS THR GLY THR VAL LEU GLY LYS GLU
SEQRES  15 B  563  LEU THR LYS LYS PHE TYR GLY LYS LYS HIS THR LYS SER
SEQRES  16 B  563  TYR TYR LEU GLY CYS SER THR GLY GLY ARG GLN GLY TRP
SEQRES  17 B  563  LYS GLU ALA GLN SER PHE PRO ASP ASP PHE ASP GLY ILE
SEQRES  18 B  563  VAL ALA GLY ALA PRO ALA MET ARG PHE ASN GLY LEU GLN
SEQRES  19 B  563  SER ARG SER GLY SER PHE TRP GLY ILE THR GLY PRO PRO
SEQRES  20 B  563  GLY ALA PRO THR HIS LEU SER PRO GLU GLU TRP ALA MET
SEQRES  21 B  563  VAL GLN LYS ASN VAL LEU VAL GLN CYS ASP GLU PRO LEU
SEQRES  22 B  563  ASP GLY VAL ALA ASP GLY ILE LEU GLU ASP PRO ASN LEU
SEQRES  23 B  563  CYS GLN TYR ARG PRO GLU ALA LEU VAL CYS SER LYS GLY
SEQRES  24 B  563  GLN THR LYS ASN CYS LEU THR GLY PRO GLN ILE GLU THR
SEQRES  25 B  563  VAL ARG LYS VAL PHE GLY PRO LEU TYR GLY ASN ASN GLY
SEQRES  26 B  563  THR TYR ILE TYR PRO ARG ILE PRO PRO GLY ALA ASP GLN
SEQRES  27 B  563  GLY PHE GLY PHE ALA ILE GLY GLU GLN PRO PHE PRO TYR
SEQRES  28 B  563  SER THR GLU TRP PHE GLN TYR VAL ILE TRP ASN ASP THR
SEQRES  29 B  563  LYS TRP ASP PRO ASN THR ILE GLY PRO ASN ASP TYR GLN
SEQRES  30 B  563  LYS ALA SER GLU VAL ASN PRO PHE ASN VAL GLU THR TRP
SEQRES  31 B  563  GLU GLY ASP LEU SER LYS PHE ARG LYS ARG GLY SER LYS
SEQRES  32 B  563  ILE ILE HIS TRP HIS GLY LEU GLU ASP GLY LEU ILE SER
SEQRES  33 B  563  SER ASP ASN SER MET GLU TYR TYR ASN HIS VAL SER ALA
SEQRES  34 B  563  THR MET GLY LEU SER ASN THR GLU LEU ASP GLU PHE TYR
SEQRES  35 B  563  ARG TYR PHE ARG VAL SER GLY CYS GLY HIS CYS SER GLY
SEQRES  36 B  563  GLY ILE GLY ALA ASN ARG ILE GLY ASN ASN ARG ALA ASN
SEQRES  37 B  563  LEU GLY GLY LYS GLU ALA LYS ASN ASN VAL LEU LEU ALA
SEQRES  38 B  563  LEU VAL LYS TRP VAL GLU GLU GLY GLN ALA PRO GLU THR
SEQRES  39 B  563  ILE THR GLY VAL ARG TYR VAL ASN GLY ALA THR THR GLY
SEQRES  40 B  563  LYS VAL GLU VAL GLU ARG ARG HIS CYS ARG TYR PRO TYR
SEQRES  41 B  563  ARG ASN VAL TRP ASP ARG LYS GLY ASN TYR LYS ASN PRO
SEQRES  42 B  563  ASP SER TRP LYS CYS GLU LEU PRO LEU GLU GLN LYS LEU
SEQRES  43 B  563  ILE SER GLU GLU ASP LEU ASN SER ALA VAL ASP HIS HIS
SEQRES  44 B  563  HIS HIS HIS HIS
HET    NAG  C   1      28
HET    NAG  C   2      27
HET    BMA  C   3      21
HET    NAG  D   1      28
HET    NAG  D   2      27
HET    BMA  D   3      21
HET    MAN  D   4      21
HET    MAN  D   5      21
HET    MAN  D   6      21
HET    NAG  E   1      28
HET    NAG  E   2      27
HET    BMA  E   3      21
HET    MAN  E   4      21
HET    MAN  E   5      21
HET    MAN  E   6      21
HET    MAN  E   7      21
HET    MAN  E   8      21
HET    MAN  E   9      21
HET    NAG  F   1      28
HET    NAG  F   2      27
HET    BMA  F   3      21
HET    MAN  F   4      21
HET    MAN  F   5      21
HET    MAN  F   6      21
HET    MAN  F   7      21
HET    MAN  F   8      21
HET    MAN  F   9      21
HET    MAN  F  10      21
HET    NAG  G   1      28
HET    NAG  G   2      27
HET    BMA  G   3      21
HET    MAN  G   4      21
HET    MAN  G   5      21
HET    MAN  G   6      21
HET    MAN  G   7      21
HET    MAN  G   8      21
HET    MAN  G   9      21
HET    MAN  G  10      21
HET    NAG  A 601      28
HET    PEG  A 602      17
HET    PEG  A 603      17
HET    EDO  A 604      10
HET    PEG  A 605      17
HET    EDO  A 606      10
HET    PGE  A 607      24
HET    NAG  A 608      28
HET     CA  A 609       1
HET    PEG  A 610      17
HET    NAG  B 601      28
HET    PEG  B 602      17
HET    EDO  B 603      10
HET    EDO  B 604      10
HET    EDO  B 605      10
HET    EDO  B 606      10
HET    EDO  B 607      10
HET    NAG  B 608      28
HET    NAG  B 609      28
HET     CA  B 610       1
HET    PEG  B 611      17
HET    PEG  B 612      17
HET    PEG  B 613      17
HETNAM     NAG 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSE
HETNAM     BMA BETA-D-MANNOPYRANOSE
HETNAM     MAN ALPHA-D-MANNOPYRANOSE
HETNAM     PEG DI(HYDROXYETHYL)ETHER
HETNAM     EDO 1,2-ETHANEDIOL
HETNAM     PGE TRIETHYLENE GLYCOL
HETNAM      CA CALCIUM ION
HETSYN     NAG N-ACETYL-BETA-D-GLUCOSAMINE; 2-ACETAMIDO-2-DEOXY-BETA-
HETSYN   2 NAG  D-GLUCOSE; 2-ACETAMIDO-2-DEOXY-D-GLUCOSE; 2-ACETAMIDO-
HETSYN   3 NAG  2-DEOXY-GLUCOSE; N-ACETYL-D-GLUCOSAMINE
HETSYN     BMA BETA-D-MANNOSE; D-MANNOSE; MANNOSE
HETSYN     MAN ALPHA-D-MANNOSE; D-MANNOSE; MANNOSE
HETSYN     EDO ETHYLENE GLYCOL
FORMUL   3  NAG    15(C8 H15 N O6)
FORMUL   3  BMA    5(C6 H12 O6)
FORMUL   4  MAN    23(C6 H12 O6)
FORMUL   9  PEG    8(C4 H10 O3)
FORMUL  11  EDO    7(C2 H6 O2)
FORMUL  14  PGE    C6 H14 O4
FORMUL  16   CA    2(CA 2+)
FORMUL  31  HOH   *606(H2 O)
HELIX    1 AA1 ASP A   36  LEU A   48  1                                  13
HELIX    2 AA2 GLN A  129  PHE A  139  1                                  11
HELIX    3 AA3 ALA A  155  TYR A  159  5                                   5
HELIX    4 AA4 ASN A  161  TYR A  170  1                                  10
HELIX    5 AA5 TYR A  170  GLY A  189  1                                  20
HELIX    6 AA6 SER A  201  PHE A  214  1                                  14
HELIX    7 AA7 ARG A  229  GLY A  245  1                                  17
HELIX    8 AA8 SER A  254  ASP A  270  1                                  17
HELIX    9 AA9 ASP A  270  GLY A  275  1                                   6
HELIX   10 AB1 ASP A  283  CYS A  287  5                                   5
HELIX   11 AB2 ARG A  290  VAL A  295  5                                   6
HELIX   12 AB3 THR A  306  PHE A  317  1                                  12
HELIX   13 AB4 ASN A  323  GLY A  325  5                                   3
HELIX   14 AB5 GLY A  339  ALA A  343  5                                   5
HELIX   15 AB6 PHE A  349  VAL A  359  1                                  11
HELIX   16 AB7 ASP A  367  ILE A  371  5                                   5
HELIX   17 AB8 GLY A  372  ASN A  383  1                                  12
HELIX   18 AB9 PRO A  384  VAL A  387  5                                   4
HELIX   19 AC1 LEU A  394  ARG A  400  1                                   7
HELIX   20 AC2 SER A  416  GLY A  432  1                                  17
HELIX   21 AC3 SER A  434  ASP A  439  1                                   6
HELIX   22 AC4 GLU A  473  ASN A  476  5                                   4
HELIX   23 AC5 ASN A  477  GLY A  489  1                                  13
HELIX   24 AC6 ASN A  502  ALA A  504  5                                   3
HELIX   25 AC7 ASN A  532  ASP A  534  5                                   3
HELIX   26 AC8 PHE B   37  LEU B   48  1                                  12
HELIX   27 AC9 GLN B  129  PHE B  139  1                                  11
HELIX   28 AD1 ALA B  155  TYR B  159  5                                   5
HELIX   29 AD2 ASN B  161  TYR B  170  1                                  10
HELIX   30 AD3 TYR B  170  GLY B  189  1                                  20
HELIX   31 AD4 SER B  201  PHE B  214  1                                  14
HELIX   32 AD5 PRO B  215  PHE B  218  5                                   4
HELIX   33 AD6 ARG B  229  GLY B  245  1                                  17
HELIX   34 AD7 SER B  254  ASP B  270  1                                  17
HELIX   35 AD8 ASP B  270  GLY B  275  1                                   6
HELIX   36 AD9 ASP B  283  CYS B  287  5                                   5
HELIX   37 AE1 ARG B  290  VAL B  295  5                                   6
HELIX   38 AE2 THR B  306  PHE B  317  1                                  12
HELIX   39 AE3 ASN B  323  GLY B  325  5                                   3
HELIX   40 AE4 PHE B  349  VAL B  359  1                                  11
HELIX   41 AE5 ASP B  367  ILE B  371  5                                   5
HELIX   42 AE6 GLY B  372  ASN B  383  1                                  12
HELIX   43 AE7 PRO B  384  VAL B  387  5                                   4
HELIX   44 AE8 LEU B  394  ARG B  400  1                                   7
HELIX   45 AE9 SER B  416  GLY B  432  1                                  17
HELIX   46 AF1 SER B  434  ASP B  439  1                                   6
HELIX   47 AF2 ASN B  477  GLY B  489  1                                  13
HELIX   48 AF3 ASN B  502  ALA B  504  5                                   3
HELIX   49 AF4 ASN B  532  ASP B  534  5                                   3
SHEET    1 AA1 9 THR A  53  VAL A  61  0
SHEET    2 AA1 9 ILE A  87  GLY A  97 -1  O  ARG A  89   N  GLU A  59
SHEET    3 AA1 9 SER A 100  PRO A 109 -1  O  LEU A 108   N  CYS A  88
SHEET    4 AA1 9 ALA A 142  ALA A 146 -1  O  THR A 143   N  TRP A 107
SHEET    5 AA1 9 PHE A 116  THR A 119  1  N  LEU A 117   O  ALA A 142
SHEET    6 AA1 9 SER A 195  CYS A 200  1  O  TYR A 196   N  PHE A 116
SHEET    7 AA1 9 GLY A 220  GLY A 224  1  O  VAL A 222   N  TYR A 197
SHEET    8 AA1 9 LYS A 403  GLY A 409  1  O  ILE A 405   N  ILE A 221
SHEET    9 AA1 9 TYR A 442  VAL A 447  1  O  PHE A 445   N  HIS A 406
SHEET    1 AA2 2 ASN A  66  THR A  68  0
SHEET    2 AA2 2 SER A  80  ILE A  82 -1  O  THR A  81   N  ILE A  67
SHEET    1 AA3 2 LEU A 320  TYR A 321  0
SHEET    2 AA3 2 TYR A 327  TYR A 329 -1  O  TYR A 329   N  LEU A 320
SHEET    1 AA4 2 THR A 494  TYR A 500  0
SHEET    2 AA4 2 VAL A 509  CYS A 516 -1  O  GLU A 510   N  ARG A 499
SHEET    1 AA5 2 ARG A 521  TRP A 524  0
SHEET    2 AA5 2 TRP A 536  GLU A 539 -1  O  GLU A 539   N  ARG A 521
SHEET    1 AA6 9 THR B  53  VAL B  61  0
SHEET    2 AA6 9 ILE B  87  GLY B  97 -1  O  PHE B  93   N  LYS B  54
SHEET    3 AA6 9 SER B 100  PRO B 109 -1  O  ALA B 106   N  VAL B  90
SHEET    4 AA6 9 ALA B 142  ALA B 146 -1  O  THR B 143   N  TRP B 107
SHEET    5 AA6 9 PHE B 116  THR B 119  1  N  LEU B 117   O  ALA B 142
SHEET    6 AA6 9 SER B 195  CYS B 200  1  O  TYR B 196   N  SER B 118
SHEET    7 AA6 9 GLY B 220  GLY B 224  1  O  VAL B 222   N  TYR B 197
SHEET    8 AA6 9 LYS B 403  GLY B 409  1  O  ILE B 405   N  ILE B 221
SHEET    9 AA6 9 TYR B 442  VAL B 447  1  O  ARG B 443   N  HIS B 406
SHEET    1 AA7 2 ASN B  66  THR B  68  0
SHEET    2 AA7 2 SER B  80  ILE B  82 -1  O  THR B  81   N  ILE B  67
SHEET    1 AA8 2 LEU B 320  TYR B 321  0
SHEET    2 AA8 2 TYR B 327  TYR B 329 -1  O  TYR B 329   N  LEU B 320
SHEET    1 AA9 2 THR B 494  TYR B 500  0
SHEET    2 AA9 2 VAL B 509  CYS B 516 -1  O  GLU B 510   N  ARG B 499
SHEET    1 AB1 2 ARG B 521  TRP B 524  0
SHEET    2 AB1 2 TRP B 536  GLU B 539 -1  O  GLU B 539   N  ARG B 521
SSBOND   1 CYS A   41    CYS A   88                          1555   1555  2.18
SSBOND   2 CYS A   76    CYS A  127                          1555   1555  2.08
SSBOND   3 CYS A  200    CYS A  453                          1555   1555  2.09
SSBOND   4 CYS A  269    CYS A  287                          1555   1555  2.10
SSBOND   5 CYS A  296    CYS A  304                          1555   1555  2.10
SSBOND   6 CYS A  516    CYS A  538                          1555   1555  2.14
SSBOND   7 CYS B   41    CYS B   88                          1555   1555  2.15
SSBOND   8 CYS B   76    CYS B  127                          1555   1555  2.07
SSBOND   9 CYS B  200    CYS B  453                          1555   1555  2.11
SSBOND  10 CYS B  269    CYS B  287                          1555   1555  2.12
SSBOND  11 CYS B  296    CYS B  304                          1555   1555  2.08
SSBOND  12 CYS B  516    CYS B  538                          1555   1555  2.11
LINK         O4  NAG C   1                 C1  NAG C   2     1555   1555  1.39
LINK         O4  NAG C   2                 C1  BMA C   3     1555   1555  1.39
LINK         O4  NAG D   1                 C1  NAG D   2     1555   1555  1.39
LINK         O4  NAG D   2                 C1  BMA D   3     1555   1555  1.39
LINK         O6  BMA D   3                 C1  MAN D   4     1555   1555  1.40
LINK         O6  MAN D   4                 C1  MAN D   5     1555   1555  1.41
LINK         O2  MAN D   5                 C1  MAN D   6     1555   1555  1.39
LINK         O4  NAG E   1                 C1  NAG E   2     1555   1555  1.39
LINK         O4  NAG E   2                 C1  BMA E   3     1555   1555  1.39
LINK         O6  BMA E   3                 C1  MAN E   4     1555   1555  1.41
LINK         O3  BMA E   3                 C1  MAN E   8     1555   1555  1.39
LINK         O6  MAN E   4                 C1  MAN E   5     1555   1555  1.39
LINK         O3  MAN E   4                 C1  MAN E   7     1555   1555  1.39
LINK         O2  MAN E   5                 C1  MAN E   6     1555   1555  1.40
LINK         O2  MAN E   8                 C1  MAN E   9     1555   1555  1.40
LINK         O4  NAG F   1                 C1  NAG F   2     1555   1555  1.39
LINK         O4  NAG F   2                 C1  BMA F   3     1555   1555  1.38
LINK         O3  BMA F   3                 C1  MAN F   4     1555   1555  1.38
LINK         O6  BMA F   3                 C1  MAN F   7     1555   1555  1.40
LINK         O2  MAN F   4                 C1  MAN F   5     1555   1555  1.40
LINK         O2  MAN F   5                 C1  MAN F   6     1555   1555  1.40
LINK         O6  MAN F   7                 C1  MAN F   8     1555   1555  1.40
LINK         O3  MAN F   7                 C1  MAN F  10     1555   1555  1.40
LINK         O2  MAN F   8                 C1  MAN F   9     1555   1555  1.40
LINK         O4  NAG G   1                 C1  NAG G   2     1555   1555  1.39
LINK         O4  NAG G   2                 C1  BMA G   3     1555   1555  1.39
LINK         O3  BMA G   3                 C1  MAN G   4     1555   1555  1.40
LINK         O6  BMA G   3                 C1  MAN G   7     1555   1555  1.40
LINK         O2  MAN G   4                 C1  MAN G   5     1555   1555  1.40
LINK         O2  MAN G   5                 C1  MAN G   6     1555   1555  1.39
LINK         O6  MAN G   7                 C1  MAN G   8     1555   1555  1.40
LINK         O3  MAN G   7                 C1  MAN G  10     1555   1555  1.39
LINK         O2  MAN G   8                 C1  MAN G   9     1555   1555  1.39
LINK         OD1 ASP A 270                CA    CA A 609     1555   1555  2.36
LINK         OD1 ASP A 274                CA    CA A 609     1555   1555  2.45
LINK         OD2 ASP A 274                CA    CA A 609     1555   1555  2.52
LINK         O   VAL A 276                CA    CA A 609     1555   1555  2.45
LINK         OD1 ASP A 278                CA    CA A 609     1555   1555  2.44
LINK         O   ILE A 280                CA    CA A 609     1555   1555  2.27
LINK        CA    CA A 609                 O   HOH A 845     1555   1555  2.33
LINK         OD1 ASP B 270                CA    CA B 610     1555   1555  2.16
LINK         OD1 ASP B 274                CA    CA B 610     1555   1555  2.55
LINK         OD2 ASP B 274                CA    CA B 610     1555   1555  2.55
LINK         O   VAL B 276                CA    CA B 610     1555   1555  2.44
LINK         OD1 ASP B 278                CA    CA B 610     1555   1555  2.45
LINK         O   ILE B 280                CA    CA B 610     1555   1555  2.40
LINK        CA    CA B 610                 O   HOH B 765     1555   1555  2.42
CISPEP   1 THR A   77    PRO A   78          0       -11.36
CISPEP   2 TYR A  518    PRO A  519          0        -0.89
CISPEP   3 THR B   77    PRO B   78          0        -4.40
CISPEP   4 ALA B  125    GLY B  126          0       -13.67
CISPEP   5 TYR B  518    PRO B  519          0         0.88
CRYST1   68.561   89.261  116.484  90.00 101.73  90.00 P 1 21 1      4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.014586  0.000000  0.003027        0.00000
SCALE2      0.000000  0.011203  0.000000        0.00000
SCALE3      0.000000  0.000000  0.008768        0.00000
TER    7807      GLU A 543
TER   15600      GLU B 543
MASTER      565    0   61   49   34    0    0    6 9134    2 1275   88
END