longtext: 9J3F-pdb

content
HEADER    TOXIN                                   08-AUG-24   9J3F
TITLE     THE STRUCTURE OF PHOSPHOLIPASE TLEB
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: TLE1 PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN;
COMPND   3 CHAIN: A;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: XANTHOMONAS ORYZAE PV. ORYZAE PXO99A;
SOURCE   3 ORGANISM_TAXID: 360094;
SOURCE   4 GENE: PXO_02034;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI 'BL21-GOLD(DE3)PLYSS AG';
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 866768
KEYWDS    HYDROLYZED PHOSPHOLIPID, TOXIN
EXPDTA    X-RAY DIFFRACTION
AUTHOR    Z.TAN
REVDAT   1   06-AUG-25 9J3F    0
JRNL        AUTH   Z.TAN
JRNL        TITL   THE STRUCTURE OF PHOSPHOLIPASE TLEB
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.90 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (1.13_2998: ???)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.90
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 46.65
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.9
REMARK   3   NUMBER OF REFLECTIONS             : 56711
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.169
REMARK   3   R VALUE            (WORKING SET) : 0.168
REMARK   3   FREE R VALUE                     : 0.197
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 3.530
REMARK   3   FREE R VALUE TEST SET COUNT      : 2000
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 46.6500 -  4.5780    0.99     3994   146  0.1561 0.1596
REMARK   3     2  4.5780 -  3.6342    1.00     3956   144  0.1312 0.1582
REMARK   3     3  3.6342 -  3.1749    1.00     3908   144  0.1447 0.1616
REMARK   3     4  3.1749 -  2.8847    1.00     3922   143  0.1616 0.1886
REMARK   3     5  2.8847 -  2.6779    1.00     3907   143  0.1613 0.2010
REMARK   3     6  2.6779 -  2.5201    1.00     3906   143  0.1665 0.2046
REMARK   3     7  2.5201 -  2.3939    1.00     3891   141  0.1657 0.2264
REMARK   3     8  2.3939 -  2.2897    1.00     3927   144  0.1803 0.2360
REMARK   3     9  2.2897 -  2.2015    1.00     3870   142  0.1851 0.2262
REMARK   3    10  2.2015 -  2.1255    1.00     3907   143  0.1870 0.2398
REMARK   3    11  2.1255 -  2.0591    1.00     3878   141  0.1918 0.2093
REMARK   3    12  2.0591 -  2.0002    1.00     3911   143  0.2237 0.2794
REMARK   3    13  2.0002 -  1.9476    1.00     3881   143  0.2783 0.2817
REMARK   3    14  1.9476 -  1.9000    1.00     3853   140  0.3388 0.3486
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.11
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.230
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 19.800
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.008           5483
REMARK   3   ANGLE     :  0.988           7420
REMARK   3   CHIRALITY :  0.052            779
REMARK   3   PLANARITY :  0.006            986
REMARK   3   DIHEDRAL  :  3.171           3294
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : 2
REMARK   3   TLS GROUP : 1
REMARK   3    SELECTION: ( CHAIN A AND RESID 35:450 )
REMARK   3    ORIGIN FOR THE GROUP (A):  -18.527    3.101  -10.920
REMARK   3    T TENSOR
REMARK   3      T11:   0.0714 T22:   0.0847
REMARK   3      T33:   0.0803 T12:   0.0005
REMARK   3      T13:  -0.0012 T23:  -0.0117
REMARK   3    L TENSOR
REMARK   3      L11:   0.1441 L22:   0.4243
REMARK   3      L33:   0.3536 L12:  -0.1405
REMARK   3      L13:  -0.0919 L23:   0.2219
REMARK   3    S TENSOR
REMARK   3      S11:   0.0176 S12:  -0.0226 S13:   0.0047
REMARK   3      S21:   0.0189 S22:   0.0540 S23:  -0.0422
REMARK   3      S31:  -0.0229 S32:   0.0326 S33:  -0.0000
REMARK   3   TLS GROUP : 2
REMARK   3    SELECTION: ( CHAIN A AND RESID 451:681 )
REMARK   3    ORIGIN FOR THE GROUP (A):  -31.796  -20.839  -33.708
REMARK   3    T TENSOR
REMARK   3      T11:   0.0918 T22:   0.0920
REMARK   3      T33:   0.1170 T12:   0.0120
REMARK   3      T13:  -0.0056 T23:  -0.0162
REMARK   3    L TENSOR
REMARK   3      L11:   0.2901 L22:   0.3527
REMARK   3      L33:   0.2917 L12:  -0.1664
REMARK   3      L13:  -0.0512 L23:   0.1175
REMARK   3    S TENSOR
REMARK   3      S11:   0.0244 S12:   0.0271 S13:  -0.0637
REMARK   3      S21:   0.0122 S22:  -0.0124 S23:   0.0567
REMARK   3      S31:  -0.0308 S32:  -0.0197 S33:   0.0000
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 9J3F COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBC ON 15-AUG-24.
REMARK 100 THE DEPOSITION ID IS D_1300050262.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 19-DEC-20
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRF
REMARK 200  BEAMLINE                       : BL18U1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9791
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 56744
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.900
REMARK 200  RESOLUTION RANGE LOW       (A) : 46.650
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9
REMARK 200  DATA REDUNDANCY                : 6.700
REMARK 200  R MERGE                    (I) : 0.18700
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 10.2000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.90
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.94
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.80
REMARK 200  R MERGE FOR SHELL          (I) : 1.71800
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 41.77
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.11
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M BIS-TRIS PH 6.8, 23% PEG3350,
REMARK 280  VAPOR DIFFUSION, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       35.18300
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 240 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 29150 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: 0.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     GLY A     2
REMARK 465     SER A     3
REMARK 465     SER A     4
REMARK 465     HIS A     5
REMARK 465     HIS A     6
REMARK 465     HIS A     7
REMARK 465     HIS A     8
REMARK 465     HIS A     9
REMARK 465     HIS A    10
REMARK 465     SER A    11
REMARK 465     SER A    12
REMARK 465     GLY A    13
REMARK 465     LEU A    14
REMARK 465     VAL A    15
REMARK 465     PRO A    16
REMARK 465     ARG A    17
REMARK 465     GLY A    18
REMARK 465     SER A    19
REMARK 465     HIS A    20
REMARK 465     MET A    21
REMARK 465     ALA A    22
REMARK 465     SER A    23
REMARK 465     MET A    24
REMARK 465     THR A    25
REMARK 465     GLY A    26
REMARK 465     GLY A    27
REMARK 465     GLN A    28
REMARK 465     GLN A    29
REMARK 465     MET A    30
REMARK 465     GLY A    31
REMARK 465     ARG A    32
REMARK 465     GLY A    33
REMARK 465     SER A    34
REMARK 465     SER A   718
REMARK 465     ALA A   719
REMARK 465     LEU A   720
REMARK 465     ALA A   721
REMARK 465     HIS A   722
REMARK 465     SER A   723
REMARK 465     ILE A   724
REMARK 465     ASP A   725
REMARK 465     ALA A   726
REMARK 465     VAL A   727
REMARK 465     ASP A   728
REMARK 465     GLU A   729
REMARK 465     THR A   730
REMARK 465     LEU A   731
REMARK 465     GLU A   732
REMARK 465     GLN A   733
REMARK 465     ILE A   734
REMARK 465     LYS A   735
REMARK 465     GLN A   736
REMARK 465     LEU A   737
REMARK 465     PRO A   738
REMARK 465     GLY A   739
REMARK 465     LYS A   740
REMARK 465     ALA A   741
REMARK 465     LYS A   742
REMARK 465     GLN A   743
REMARK 465     ALA A   744
REMARK 465     VAL A   745
REMARK 465     GLY A   746
REMARK 465     ASP A   747
REMARK 465     ALA A   748
REMARK 465     VAL A   749
REMARK 465     GLU A   750
REMARK 465     SER A   751
REMARK 465     ALA A   752
REMARK 465     VAL A   753
REMARK 465     ASP A   754
REMARK 465     SER A   755
REMARK 465     ALA A   756
REMARK 465     VAL A   757
REMARK 465     GLU A   758
REMARK 465     ALA A   759
REMARK 465     GLY A   760
REMARK 465     LYS A   761
REMARK 465     ASP A   762
REMARK 465     PHE A   763
REMARK 465     VAL A   764
REMARK 465     GLY A   765
REMARK 465     ASP A   766
REMARK 465     GLN A   767
REMARK 465     VAL A   768
REMARK 465     ARG A   769
REMARK 465     LYS A   770
REMARK 465     LEU A   771
REMARK 465     ILE A   772
REMARK 465     PRO A   773
REMARK 465     SER A   774
REMARK 465     GLY A   775
REMARK 465     LEU A   776
REMARK 465     PRO A   777
REMARK 465     ARG A   778
REMARK 465     MET A   779
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470   M RES CSSEQI  ATOMS
REMARK 470     GLU A  35    CG   CD   OE1  OE2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    PHE A 129       54.19   -146.56
REMARK 500    SER A 276     -131.53     66.50
REMARK 500    ASP A 316       72.47     38.17
REMARK 500    ALA A 319      -76.34    -94.12
REMARK 500    HIS A 333       53.14   -104.25
REMARK 500    LYS A 509      118.11   -161.18
REMARK 500    TYR A 598      -51.51   -128.48
REMARK 500    ASP A 696     -158.24   -144.68
REMARK 500    PHE A 704      -99.53   -112.20
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A1413        DISTANCE =  6.38 ANGSTROMS
REMARK 525    HOH A1414        DISTANCE =  7.26 ANGSTROMS
DBREF1 9J3F A   60   779  UNP                  A0A0K0GNK3_XANOP
DBREF2 9J3F A     A0A0K0GNK3                          1         720
SEQADV 9J3F MET A    1  UNP  A0A0K0GNK           INITIATING METHIONINE
SEQADV 9J3F GLY A    2  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A    3  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A    4  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F HIS A    5  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F HIS A    6  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F HIS A    7  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F HIS A    8  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F HIS A    9  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F HIS A   10  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A   11  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A   12  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLY A   13  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F LEU A   14  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F VAL A   15  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F PRO A   16  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F ARG A   17  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLY A   18  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A   19  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F HIS A   20  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F MET A   21  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F ALA A   22  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A   23  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F MET A   24  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F THR A   25  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLY A   26  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLY A   27  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLN A   28  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLN A   29  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F MET A   30  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLY A   31  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F ARG A   32  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLY A   33  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A   34  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLU A   35  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F PHE A   36  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F MET A   37  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLN A   38  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F VAL A   39  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A   40  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F PHE A   41  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F ALA A   42  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F PRO A   43  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F LEU A   44  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F CYS A   45  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F PRO A   46  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLU A   47  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F ASN A   48  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F PRO A   49  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F LEU A   50  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLN A   51  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F LEU A   52  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F SER A   53  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F ARG A   54  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F ALA A   55  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLN A   56  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLN A   57  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F ALA A   58  UNP  A0A0K0GNK           EXPRESSION TAG
SEQADV 9J3F GLU A   59  UNP  A0A0K0GNK           EXPRESSION TAG
SEQRES   1 A  779  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY
SEQRES   2 A  779  LEU VAL PRO ARG GLY SER HIS MET ALA SER MET THR GLY
SEQRES   3 A  779  GLY GLN GLN MET GLY ARG GLY SER GLU PHE MET GLN VAL
SEQRES   4 A  779  SER PHE ALA PRO LEU CYS PRO GLU ASN PRO LEU GLN LEU
SEQRES   5 A  779  SER ARG ALA GLN GLN ALA GLU MET MET SER ALA GLY LEU
SEQRES   6 A  779  GLY ARG PRO GLN ASP SER CYS CYS ILE ASP LEU ARG TRP
SEQRES   7 A  779  GLY PHE PHE PHE ASP GLY THR ASN ASN ASN PHE HIS ARG
SEQRES   8 A  779  ASP GLN PRO LYS LYS ALA HIS SER ASN VAL ALA ARG LEU
SEQRES   9 A  779  TYR ASP ILE PHE GLU ALA ASP ARG ARG LYS PRO GLU PHE
SEQRES  10 A  779  VAL GLY ARG TYR ALA ALA GLY VAL GLY THR ALA PHE LYS
SEQRES  11 A  779  ASP GLU VAL GLY ASP GLN GLY LEU GLY ILE GLN GLU LYS
SEQRES  12 A  779  ALA GLY LEU ALA ALA GLY TRP GLY GLY GLU ALA ARG ILE
SEQRES  13 A  779  CYS TRP ALA LEU LEU LYS PHE LEU ASP ASN LEU ASN TYR
SEQRES  14 A  779  TYR PHE GLU ARG ILE ASP LEU GLY GLU ALA LEU GLY GLN
SEQRES  15 A  779  LYS ASP PRO ALA THR VAL ARG ARG MET ALA GLN ASP MET
SEQRES  16 A  779  THR ILE PRO SER MET GLU LEU ARG LYS ILE ALA GLY ASP
SEQRES  17 A  779  GLU THR GLU MET LEU ARG GLN ILE SER MET MET ALA SER
SEQRES  18 A  779  LEU GLN SER LEU THR ALA THR ALA LEU ASN LEU PRO ASN
SEQRES  19 A  779  HIS ARG GLY ARG ARG ALA VAL LEU ALA GLU ARG ARG ALA
SEQRES  20 A  779  GLN LEU ARG GLN ARG VAL GLN THR TRP GLN ARG ALA GLN
SEQRES  21 A  779  PRO LYS PRO LYS LEU ARG SER ILE ARG VAL SER VAL PHE
SEQRES  22 A  779  GLY PHE SER ARG GLY ALA ALA GLU ALA ARG VAL PHE CYS
SEQRES  23 A  779  SER TRP LEU LYS ASP ALA CYS ASP GLY GLY GLY GLY GLU
SEQRES  24 A  779  LEU THR LEU CYS GLY ILE PRO VAL GLN LEU ASP LEU LEU
SEQRES  25 A  779  GLY ILE PHE ASP THR VAL ALA SER VAL GLY LEU ALA ASN
SEQRES  26 A  779  SER SER ARG LEU TRP SER GLY HIS GLY GLY TYR ALA SER
SEQRES  27 A  779  GLU ASP ASP LEU ARG ILE ALA PRO TYR VAL ARG ARG CYS
SEQRES  28 A  779  VAL HIS LEU VAL ALA ALA HIS GLU VAL ARG GLY SER PHE
SEQRES  29 A  779  PRO LEU ASP ALA ALA ALA GLY VAL ASN GLY GLU GLU VAL
SEQRES  30 A  779  VAL TYR PRO GLY VAL HIS SER ASP VAL GLY GLY GLY TYR
SEQRES  31 A  779  GLU PRO GLY GLU GLN GLY LYS ALA PHE ILE GLY ASP SER
SEQRES  32 A  779  ILE ASP ASP SER ALA LYS LEU SER GLN ILE ALA LEU CYS
SEQRES  33 A  779  HIS MET TYR ARG GLU ALA MET ALA ALA GLY VAL PRO LEU
SEQRES  34 A  779  ASN LEU SER ALA SER ARG LEU SER LYS GLU THR LYS ALA
SEQRES  35 A  779  ALA PHE LYS VAL ASP LYS GLY LEU ILE ASP ALA PHE ASN
SEQRES  36 A  779  GLY TYR VAL ALA ALA THR GLY SER ILE LYS ALA SER THR
SEQRES  37 A  779  THR VAL ALA LEU THR GLN ALA HIS TYR ALA LEU TYR LEU
SEQRES  38 A  779  ARG TRP ARG ARG LEU ARG LEU ASP ASP THR ALA PRO ASP
SEQRES  39 A  779  GLY MET ALA GLN GLN PRO PHE VAL THR ARG ALA ARG THR
SEQRES  40 A  779  TYR LYS ALA GLN ASP VAL THR ASP LEU LEU GLN THR ASN
SEQRES  41 A  779  ALA GLU LEU ARG GLN GLU TRP ALA ALA LEU GLN GLN ASP
SEQRES  42 A  779  GLU LYS ASP ALA ALA TYR SER SER GLU ALA SER VAL ALA
SEQRES  43 A  779  HIS VAL LEU ARG SER THR LEU ALA PRO ILE ALA ALA ARG
SEQRES  44 A  779  ASP ASP ILE VAL ALA LEU VAL TRP GLY GLU LYS MET THR
SEQRES  45 A  779  GLN TRP ARG GLU VAL LYS PRO ALA TRP ASN ASP LEU SER
SEQRES  46 A  779  PRO LEU ASP ARG ARG ILE VAL ARG LEU HIS ASP ASP TYR
SEQRES  47 A  779  SER HIS ASP SER ARG ALA TRP PHE LYS PRO PHE GLY ALA
SEQRES  48 A  779  ALA SER GLU GLU ALA TRP LYS ARG GLN TYR ARG GLN ARG
SEQRES  49 A  779  MET ASN ARG LEU GLU ALA GLN ASP ASN ALA TRP GLN GLN
SEQRES  50 A  779  TRP ASN ARG ASP VAL GLN PRO VAL ILE ASP ASP ALA VAL
SEQRES  51 A  779  ARG LYS ALA GLN LYS HIS PRO GLY SER PHE GLN PRO THR
SEQRES  52 A  779  PRO GLU VAL ARG PRO MET PRO PRO LEU VAL ALA GLY GLN
SEQRES  53 A  779  ASP LEU LYS ASP LEU LYS GLN TRP ARG SER ASN GLY GLY
SEQRES  54 A  779  VAL ILE PRO THR GLU GLN ASP GLY ARG GLU SER TYR GLY
SEQRES  55 A  779  MET PHE GLY PHE LEU ARG TRP ARG THR ILE PHE VAL PRO
SEQRES  56 A  779  GLU LYS SER ALA LEU ALA HIS SER ILE ASP ALA VAL ASP
SEQRES  57 A  779  GLU THR LEU GLU GLN ILE LYS GLN LEU PRO GLY LYS ALA
SEQRES  58 A  779  LYS GLN ALA VAL GLY ASP ALA VAL GLU SER ALA VAL ASP
SEQRES  59 A  779  SER ALA VAL GLU ALA GLY LYS ASP PHE VAL GLY ASP GLN
SEQRES  60 A  779  VAL ARG LYS LEU ILE PRO SER GLY LEU PRO ARG MET
HET    GOL  A 801       6
HETNAM     GOL GLYCEROL
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL
FORMUL   2  GOL    C3 H8 O3
FORMUL   3  HOH   *514(H2 O)
HELIX    1 AA1 SER A   53  GLY A   64  1                                  12
HELIX    2 AA2 ASN A   88  GLN A   93  1                                   6
HELIX    3 AA3 PRO A   94  LYS A   96  5                                   3
HELIX    4 AA4 SER A   99  PHE A  108  1                                  10
HELIX    5 AA5 PHE A  129  GLY A  134  1                                   6
HELIX    6 AA6 LEU A  138  GLY A  149  1                                  12
HELIX    7 AA7 GLY A  151  ARG A  173  1                                  23
HELIX    8 AA8 ASP A  175  LEU A  180  1                                   6
HELIX    9 AA9 LYS A  183  ALA A  192  1                                  10
HELIX   10 AB1 GLN A  193  ILE A  197  5                                   5
HELIX   11 AB2 PRO A  198  GLY A  207  1                                  10
HELIX   12 AB3 GLY A  207  SER A  221  1                                  15
HELIX   13 AB4 ASN A  234  GLN A  260  1                                  27
HELIX   14 AB5 SER A  276  CYS A  293  1                                  18
HELIX   15 AB6 SER A  338  ARG A  343  1                                   6
HELIX   16 AB7 VAL A  382  GLY A  388  1                                   7
HELIX   17 AB8 GLY A  393  LYS A  397  5                                   5
HELIX   18 AB9 ASP A  406  LYS A  409  5                                   4
HELIX   19 AC1 LEU A  410  ALA A  425  1                                  16
HELIX   20 AC2 SER A  437  ALA A  443  1                                   7
HELIX   21 AC3 ASP A  447  THR A  461  1                                  15
HELIX   22 AC4 THR A  468  ARG A  487  1                                  20
HELIX   23 AC5 GLY A  495  GLN A  498  5                                   4
HELIX   24 AC6 GLN A  499  LYS A  509  1                                  11
HELIX   25 AC7 LYS A  509  GLU A  534  1                                  26
HELIX   26 AC8 ALA A  543  LEU A  553  1                                  11
HELIX   27 AC9 ALA A  554  ALA A  557  5                                   4
HELIX   28 AD1 ARG A  559  ASN A  582  1                                  24
HELIX   29 AD2 ASP A  588  TYR A  598  1                                  11
HELIX   30 AD3 ASP A  601  PHE A  606  1                                   6
HELIX   31 AD4 SER A  613  HIS A  656  1                                  44
HELIX   32 AD5 ALA A  674  GLY A  688  1                                  15
SHEET    1 AA1 7 MET A  37  PHE A  41  0
SHEET    2 AA1 7 GLY A 374  TYR A 379  1  O  VAL A 378   N  SER A  40
SHEET    3 AA1 7 CYS A 351  ALA A 356  1  N  VAL A 355   O  TYR A 379
SHEET    4 AA1 7 ILE A 305  PHE A 315  1  N  LEU A 312   O  VAL A 352
SHEET    5 AA1 7 LYS A 264  PHE A 275  1  N  VAL A 270   O  GLN A 308
SHEET    6 AA1 7 CYS A  73  PHE A  82  1  N  LEU A  76   O  ARG A 269
SHEET    7 AA1 7 PHE A 117  ALA A 122  1  O  ALA A 122   N  PHE A  81
SHEET    1 AA2 5 MET A  37  PHE A  41  0
SHEET    2 AA2 5 GLY A 374  TYR A 379  1  O  VAL A 378   N  SER A  40
SHEET    3 AA2 5 CYS A 351  ALA A 356  1  N  VAL A 355   O  TYR A 379
SHEET    4 AA2 5 ILE A 305  PHE A 315  1  N  LEU A 312   O  VAL A 352
SHEET    5 AA2 5 THR A 301  LEU A 302 -1  N  LEU A 302   O  ILE A 305
SHEET    1 AA3 2 LEU A 366  ASP A 367  0
SHEET    2 AA3 2 ILE A 712  PHE A 713  1  O  PHE A 713   N  LEU A 366
SHEET    1 AA4 2 PHE A 399  ILE A 400  0
SHEET    2 AA4 2 SER A 403  ILE A 404 -1  O  SER A 403   N  ILE A 400
SSBOND   1 CYS A   72    CYS A   73                          1555   1555  2.57
CISPEP   1 LYS A  262    PRO A  263          0        -2.65
CRYST1   66.640   70.366   78.775  90.00  99.26  90.00 P 1 21 1      2
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.015006  0.000000  0.002447        0.00000
SCALE2      0.000000  0.014211  0.000000        0.00000
SCALE3      0.000000  0.000000  0.012862        0.00000
TER    5364      LYS A 717
MASTER      377    0    1   32   16    0    0    6 5883    1    8   60
END