longtext: 9KL9-pdb

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HEADER    HYDROLASE                               14-NOV-24   9KL9
TITLE     CRYSTAL STRUCTURE OF A MUTANT POLY(ETHYLENE TEREPHTHALATE) HYDROLASE
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: POLY(ETHYLENE TEREPHTHALATE) HYDROLASE;
COMPND   3 CHAIN: A, B;
COMPND   4 EC: 3.1.1.101;
COMPND   5 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: BACTERIUM HR29;
SOURCE   3 ORGANISM_TAXID: 2035424;
SOURCE   4 GENE: HRBIN29_00073;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008
KEYWDS    HYDROLASE, MUTANT, DIMER
EXPDTA    X-RAY DIFFRACTION
AUTHOR    H.WANG,Y.FENG,X.Y.DU
REVDAT   1   01-OCT-25 9KL9    0
JRNL        AUTH   H.WANG,Y.CUN,M.WANG,X.DU,Z.YANG,H.WANG,J.ZHANG,P.WANG,
JRNL        AUTH 2 Y.FENG,Y.ZHU
JRNL        TITL   COMPUTATIONAL LOOP RECONSTRUCTION BASED DESIGN OF EFFICIENT
JRNL        TITL 2 PET HYDROLASES.
JRNL        REF    COMMUN BIOL                   V.   8   934 2025
JRNL        REFN                   ESSN 2399-3642
JRNL        PMID   40527955
JRNL        DOI    10.1038/S42003-025-08364-6
REMARK   2
REMARK   2 RESOLUTION.    2.90 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.21.1_5286
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : GEOSTD + MONOMER LIBRARY + CDL V1.2
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.90
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 32.17
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.960
REMARK   3   COMPLETENESS FOR RANGE        (%) : 97.2
REMARK   3   NUMBER OF REFLECTIONS             : 9942
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.197
REMARK   3   R VALUE            (WORKING SET) : 0.195
REMARK   3   FREE R VALUE                     : 0.235
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.010
REMARK   3   FREE R VALUE TEST SET COUNT      : 498
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 32.1700 -  4.6000    1.00     2409   136  0.1739 0.1835
REMARK   3     2  4.6000 -  3.6500    0.99     2387   138  0.1666 0.2200
REMARK   3     3  3.6500 -  3.1900    0.97     2361   130  0.2177 0.2824
REMARK   3     4  3.1900 -  2.9000    0.93     2287    94  0.2476 0.3100
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.10
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.351
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 21.552
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 24.45
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 21.63
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.003           4016
REMARK   3   ANGLE     :  0.620           5503
REMARK   3   CHIRALITY :  0.045            630
REMARK   3   PLANARITY :  0.007            721
REMARK   3   DIHEDRAL  : 12.799           1431
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 9KL9 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBC ON 20-NOV-24.
REMARK 100 THE DEPOSITION ID IS D_1300053755.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 13-JUN-24
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : SSRF
REMARK 200  BEAMLINE                       : BL02U1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97918
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER2 S 9M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XIA2
REMARK 200  DATA SCALING SOFTWARE          : XIA2
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 10117
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.899
REMARK 200  RESOLUTION RANGE LOW       (A) : 32.170
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.2
REMARK 200  DATA REDUNDANCY                : 3.500
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 6.1900
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.90
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.19
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHENIX
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 39.41
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.03
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 2.5 M NACL, 100 MM IMIDAZOLE PH8.0,
REMARK 280  VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     GLN A     2
REMARK 465     GLU A   262
REMARK 465     LEU A   263
REMARK 465     GLU A   264
REMARK 465     HIS A   265
REMARK 465     HIS A   266
REMARK 465     HIS A   267
REMARK 465     HIS A   268
REMARK 465     HIS A   269
REMARK 465     HIS A   270
REMARK 465     MET B     1
REMARK 465     GLN B     2
REMARK 465     LEU B   261
REMARK 465     GLU B   262
REMARK 465     LEU B   263
REMARK 465     GLU B   264
REMARK 465     HIS B   265
REMARK 465     HIS B   266
REMARK 465     HIS B   267
REMARK 465     HIS B   268
REMARK 465     HIS B   269
REMARK 465     HIS B   270
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    THR A  63      -14.93     65.80
REMARK 500    SER A 132     -112.29     66.38
REMARK 500    THR A 155       57.46     39.93
REMARK 500    HIS A 158      141.79   -170.47
REMARK 500    ASN A 185      -81.24   -124.44
REMARK 500    THR B  63       -1.43     68.68
REMARK 500    SER B 132     -122.68     61.00
REMARK 500    THR B 155       59.71     34.83
REMARK 500    HIS B 158      141.84   -170.57
REMARK 500    ASN B 185      -80.15   -125.03
REMARK 500
REMARK 500 REMARK: NULL
DBREF1 9KL9 A    2   260  UNP                  A0A2H5Z9R5_UNCXX
DBREF2 9KL9 A     A0A2H5Z9R5                         35         293
DBREF1 9KL9 B    2   260  UNP                  A0A2H5Z9R5_UNCXX
DBREF2 9KL9 B     A0A2H5Z9R5                         35         293
SEQADV 9KL9 MET A    1  UNP  A0A2H5Z9R           INITIATING METHIONINE
SEQADV 9KL9 ASN A  185  UNP  A0A2H5Z9R HIS   218 CONFLICT
SEQADV 9KL9 MET A  189  UNP  A0A2H5Z9R PHE   222 CONFLICT
SEQADV 9KL9 THR A  210  UNP  A0A2H5Z9R PHE   243 CONFLICT
SEQADV 9KL9 LEU A  261  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 GLU A  262  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 LEU A  263  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 GLU A  264  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS A  265  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS A  266  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS A  267  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS A  268  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS A  269  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS A  270  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 MET B    1  UNP  A0A2H5Z9R           INITIATING METHIONINE
SEQADV 9KL9 ASN B  185  UNP  A0A2H5Z9R HIS   218 CONFLICT
SEQADV 9KL9 MET B  189  UNP  A0A2H5Z9R PHE   222 CONFLICT
SEQADV 9KL9 THR B  210  UNP  A0A2H5Z9R PHE   243 CONFLICT
SEQADV 9KL9 LEU B  261  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 GLU B  262  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 LEU B  263  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 GLU B  264  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS B  265  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS B  266  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS B  267  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS B  268  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS B  269  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQADV 9KL9 HIS B  270  UNP  A0A2H5Z9R           EXPRESSION TAG
SEQRES   1 A  270  MET GLN SER ASN PRO TYR GLN ARG GLY PRO ASN PRO THR
SEQRES   2 A  270  ARG SER ALA LEU THR THR ASP GLY PRO PHE SER VAL ALA
SEQRES   3 A  270  THR TYR SER VAL SER ARG LEU SER VAL SER GLY PHE GLY
SEQRES   4 A  270  GLY GLY VAL ILE TYR TYR PRO THR GLY THR THR LEU THR
SEQRES   5 A  270  PHE GLY GLY ILE ALA MET SER PRO GLY TYR THR ALA ASP
SEQRES   6 A  270  ALA SER SER LEU ALA TRP LEU GLY ARG ARG LEU ALA SER
SEQRES   7 A  270  HIS GLY PHE VAL VAL ILE VAL ILE ASN THR ASN SER ARG
SEQRES   8 A  270  LEU ASP PHE PRO ASP SER ARG ALA SER GLN LEU SER ALA
SEQRES   9 A  270  ALA LEU ASN TYR LEU ARG THR SER SER PRO SER ALA VAL
SEQRES  10 A  270  ARG ALA ARG LEU ASP ALA ASN ARG LEU ALA VAL ALA GLY
SEQRES  11 A  270  HIS SER MET GLY GLY GLY ALA THR LEU ARG ILE SER GLU
SEQRES  12 A  270  GLN ILE PRO THR LEU LYS ALA GLY VAL PRO LEU THR PRO
SEQRES  13 A  270  TRP HIS THR ASP LYS THR PHE ASN THR PRO VAL PRO GLN
SEQRES  14 A  270  LEU ILE VAL GLY ALA GLU ALA ASP THR VAL ALA PRO VAL
SEQRES  15 A  270  SER GLN ASN ALA ILE PRO MET TYR GLN ASN LEU PRO SER
SEQRES  16 A  270  THR THR PRO LYS VAL TYR VAL GLU LEU ASP ASN ALA THR
SEQRES  17 A  270  HIS THR ALA PRO ASN SER PRO ASN ALA ALA ILE SER VAL
SEQRES  18 A  270  TYR THR ILE SER TRP MET LYS LEU TRP VAL ASP ASN ASP
SEQRES  19 A  270  THR ARG TYR ARG GLN PHE LEU CYS ASN VAL ASN ASP PRO
SEQRES  20 A  270  ALA LEU SER ASP PHE ARG SER ASN ASN ARG HIS CYS GLN
SEQRES  21 A  270  LEU GLU LEU GLU HIS HIS HIS HIS HIS HIS
SEQRES   1 B  270  MET GLN SER ASN PRO TYR GLN ARG GLY PRO ASN PRO THR
SEQRES   2 B  270  ARG SER ALA LEU THR THR ASP GLY PRO PHE SER VAL ALA
SEQRES   3 B  270  THR TYR SER VAL SER ARG LEU SER VAL SER GLY PHE GLY
SEQRES   4 B  270  GLY GLY VAL ILE TYR TYR PRO THR GLY THR THR LEU THR
SEQRES   5 B  270  PHE GLY GLY ILE ALA MET SER PRO GLY TYR THR ALA ASP
SEQRES   6 B  270  ALA SER SER LEU ALA TRP LEU GLY ARG ARG LEU ALA SER
SEQRES   7 B  270  HIS GLY PHE VAL VAL ILE VAL ILE ASN THR ASN SER ARG
SEQRES   8 B  270  LEU ASP PHE PRO ASP SER ARG ALA SER GLN LEU SER ALA
SEQRES   9 B  270  ALA LEU ASN TYR LEU ARG THR SER SER PRO SER ALA VAL
SEQRES  10 B  270  ARG ALA ARG LEU ASP ALA ASN ARG LEU ALA VAL ALA GLY
SEQRES  11 B  270  HIS SER MET GLY GLY GLY ALA THR LEU ARG ILE SER GLU
SEQRES  12 B  270  GLN ILE PRO THR LEU LYS ALA GLY VAL PRO LEU THR PRO
SEQRES  13 B  270  TRP HIS THR ASP LYS THR PHE ASN THR PRO VAL PRO GLN
SEQRES  14 B  270  LEU ILE VAL GLY ALA GLU ALA ASP THR VAL ALA PRO VAL
SEQRES  15 B  270  SER GLN ASN ALA ILE PRO MET TYR GLN ASN LEU PRO SER
SEQRES  16 B  270  THR THR PRO LYS VAL TYR VAL GLU LEU ASP ASN ALA THR
SEQRES  17 B  270  HIS THR ALA PRO ASN SER PRO ASN ALA ALA ILE SER VAL
SEQRES  18 B  270  TYR THR ILE SER TRP MET LYS LEU TRP VAL ASP ASN ASP
SEQRES  19 B  270  THR ARG TYR ARG GLN PHE LEU CYS ASN VAL ASN ASP PRO
SEQRES  20 B  270  ALA LEU SER ASP PHE ARG SER ASN ASN ARG HIS CYS GLN
SEQRES  21 B  270  LEU GLU LEU GLU HIS HIS HIS HIS HIS HIS
HELIX    1 AA1 ARG A   14  THR A   19  5                                   6
HELIX    2 AA2 ASP A   65  SER A   68  5                                   4
HELIX    3 AA3 LEU A   69  HIS A   79  1                                  11
HELIX    4 AA4 PHE A   94  SER A  112  1                                  19
HELIX    5 AA5 PRO A  114  ARG A  120  1                                   7
HELIX    6 AA6 SER A  132  ILE A  145  1                                  14
HELIX    7 AA7 ASN A  185  LEU A  193  1                                   9
HELIX    8 AA8 THR A  210  SER A  214  5                                   5
HELIX    9 AA9 ASN A  216  ASP A  232  1                                  17
HELIX   10 AB1 ASP A  234  LEU A  241  5                                   8
HELIX   11 AB2 SER B   15  THR B   19  5                                   5
HELIX   12 AB3 SER B   31  VAL B   35  5                                   5
HELIX   13 AB4 ASP B   65  SER B   68  5                                   4
HELIX   14 AB5 LEU B   69  HIS B   79  1                                  11
HELIX   15 AB6 PHE B   94  SER B  112  1                                  19
HELIX   16 AB7 PRO B  114  ALA B  119  1                                   6
HELIX   17 AB8 SER B  132  ILE B  145  1                                  14
HELIX   18 AB9 ASN B  185  LEU B  193  1                                   9
HELIX   19 AC1 THR B  210  SER B  214  5                                   5
HELIX   20 AC2 ASN B  216  ASP B  232  1                                  17
HELIX   21 AC3 ASP B  234  LEU B  241  5                                   8
SHEET    1 AA1 6 SER A  24  VAL A  30  0
SHEET    2 AA1 6 GLY A  41  THR A  47 -1  O  TYR A  45   N  ALA A  26
SHEET    3 AA1 6 VAL A  82  ILE A  86 -1  O  VAL A  83   N  TYR A  44
SHEET    4 AA1 6 PHE A  53  SER A  59  1  N  MET A  58   O  ILE A  86
SHEET    5 AA1 6 LEU A 121  HIS A 131  1  O  ARG A 125   N  GLY A  55
SHEET    6 AA1 6 ALA A 150  LEU A 154  1  O  LEU A 154   N  GLY A 130
SHEET    1 AA2 3 GLN A 169  ALA A 174  0
SHEET    2 AA2 3 LYS A 199  LEU A 204  1  O  VAL A 200   N  ILE A 171
SHEET    3 AA2 3 LEU A 249  SER A 254 -1  O  SER A 250   N  GLU A 203
SHEET    1 AA3 6 SER B  24  VAL B  30  0
SHEET    2 AA3 6 GLY B  41  THR B  47 -1  O  ILE B  43   N  TYR B  28
SHEET    3 AA3 6 VAL B  82  ILE B  86 -1  O  VAL B  83   N  TYR B  44
SHEET    4 AA3 6 PHE B  53  SER B  59  1  N  ILE B  56   O  ILE B  84
SHEET    5 AA3 6 LEU B 121  HIS B 131  1  O  ARG B 125   N  GLY B  55
SHEET    6 AA3 6 GLY B 151  LEU B 154  1  O  LEU B 154   N  GLY B 130
SHEET    1 AA4 3 GLN B 169  ALA B 174  0
SHEET    2 AA4 3 LYS B 199  LEU B 204  1  O  VAL B 202   N  ILE B 171
SHEET    3 AA4 3 LEU B 249  SER B 254 -1  O  ASP B 251   N  GLU B 203
SSBOND   1 CYS A  242    CYS A  259                          1555   1555  2.03
SSBOND   2 CYS B  242    CYS B  259                          1555   1555  2.03
CRYST1   43.713   51.615   56.679 103.23 103.61  96.66 P 1           2
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.022876  0.002672  0.006481        0.00000
SCALE2      0.000000  0.019506  0.005370        0.00000
SCALE3      0.000000  0.000000  0.018828        0.00000
TER    1963      LEU A 261
TER    3918      GLN B 260
MASTER      242    0    0   21   18    0    0    6 3916    2    4   42
END