longtext: 9N8R-pdb

content
HEADER    HYDROLASE                               09-FEB-25   9N8R
TITLE     LIPASE 1 FROM PSEUDOMONAS CHLORORAPHIS PA23
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: LIPASE 1;
COMPND   3 CHAIN: A;
COMPND   4 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: PSEUDOMONAS CHLORORAPHIS;
SOURCE   3 ORGANISM_TAXID: 587753;
SOURCE   4 STRAIN: PA23;
SOURCE   5 GENE: HLB40_08715;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: EXPRESSION VECTOR;
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PET-21(+)
KEYWDS    A/B HYDROLASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    S.ROYAN,S.NEWTON,A.ARDEVOL,A.T.CAPUTO
REVDAT   1   11-FEB-26 9N8R    0
JRNL        AUTH   S.ROYAN,S.NEWTON,A.ARDEVOL,A.T.CAPUTO
JRNL        TITL   STRUCTURE OF BIOPLASTIC DEGRADING ENZYME LIP1 FROM
JRNL        TITL 2 PSEUDOMONAS CHLORORAPHIS PA23 TO 1.62 ANGSTROMS
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   1
REMARK   1 REFERENCE 1
REMARK   1  AUTH   D.LIEBSCHNER,P.V.AFONINE,M.L.BAKER,G.BUNKOCZI,V.B.CHEN,
REMARK   1  AUTH 2 T.I.CROLL,B.HINTZE,L.W.HUNG,S.JAIN,A.J.MCCOY,N.W.MORIARTY,
REMARK   1  AUTH 3 R.D.OEFFNER,B.K.POON,M.G.PRISANT,R.J.READ,J.S.RICHARDSON,
REMARK   1  AUTH 4 D.C.RICHARDSON,M.D.SAMMITO,O.V.SOBOLEV,D.H.STOCKWELL,
REMARK   1  AUTH 5 T.C.TERWILLIGER,A.G.URZHUMTSEV,L.L.VIDEAU,C.J.WILLIAMS,
REMARK   1  AUTH 6 P.D.ADAMS
REMARK   1  TITL   MACROMOLECULAR STRUCTURE DETERMINATION USING X-RAYS,
REMARK   1  TITL 2 NEUTRONS AND ELECTRONS: RECENT DEVELOPMENTS IN PHENIX
REMARK   1  REF    ACTA CRYSTALLOGR D STRUCT                  2019
REMARK   1  REF  2 BIOL
REMARK   1  REFN
REMARK   1  PMID   31588918
REMARK   1  DOI    10.1107/S2059798319011471
REMARK   2
REMARK   2 RESOLUTION.    1.62 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.21.2_5419
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : GEOSTD + MONOMER LIBRARY + CDL V1.2
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.62
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 42.51
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8
REMARK   3   NUMBER OF REFLECTIONS             : 36337
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.156
REMARK   3   R VALUE            (WORKING SET) : 0.155
REMARK   3   FREE R VALUE                     : 0.182
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.010
REMARK   3   FREE R VALUE TEST SET COUNT      : 1819
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 42.5100 -  3.8100    1.00     2851   139  0.1805 0.1890
REMARK   3     2  3.8100 -  3.0200    1.00     2720   132  0.1383 0.1477
REMARK   3     3  3.0200 -  2.6400    1.00     2689   142  0.1375 0.1618
REMARK   3     4  2.6400 -  2.4000    1.00     2687   131  0.1324 0.1663
REMARK   3     5  2.4000 -  2.2300    1.00     2656   137  0.1378 0.1857
REMARK   3     6  2.2300 -  2.1000    1.00     2606   164  0.1350 0.1756
REMARK   3     7  2.1000 -  1.9900    1.00     2666   116  0.1376 0.1711
REMARK   3     8  1.9900 -  1.9000    1.00     2626   148  0.1433 0.1949
REMARK   3     9  1.9000 -  1.8300    1.00     2619   134  0.1658 0.2159
REMARK   3    10  1.8300 -  1.7700    1.00     2646   156  0.1873 0.2177
REMARK   3    11  1.7700 -  1.7100    1.00     2572   145  0.1907 0.2202
REMARK   3    12  1.7100 -  1.6600    1.00     2642   143  0.2023 0.2221
REMARK   3    13  1.6600 -  1.6200    0.97     2538   132  0.2300 0.2977
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.10
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.141
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 16.567
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 17.63
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 20.79
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.008           2208
REMARK   3   ANGLE     :  0.971           3018
REMARK   3   CHIRALITY :  0.059            337
REMARK   3   PLANARITY :  0.010            394
REMARK   3   DIHEDRAL  : 15.356            786
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 9N8R COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 11-FEB-25.
REMARK 100 THE DEPOSITION ID IS D_1000292622.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 28-MAR-24
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 7.1
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : AUSTRALIAN SYNCHROTRON
REMARK 200  BEAMLINE                       : MX2
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.0
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER X 16M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS 0.7.8
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 36418
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.620
REMARK 200  RESOLUTION RANGE LOW       (A) : 43.970
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8
REMARK 200  DATA REDUNDANCY                : 13.40
REMARK 200  R MERGE                    (I) : 0.12900
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 10.9000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.62
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.65
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 1.61900
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 45.79
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.27
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M MES PH 7.1, 20.79% (W/V) PEG
REMARK 280  6000, 0.12 M CALCIUM CHLORIDE, VAPOR DIFFUSION, SITTING DROP,
REMARK 280  TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z
REMARK 290       3555   -X+1/2,Y+1/2,-Z
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       34.29750
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       37.65450
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       34.29750
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       37.65450
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375 CA    CA A 301  LIES ON A SPECIAL POSITION.
REMARK 375      HOH A 502  LIES ON A SPECIAL POSITION.
REMARK 375      HOH A 565  LIES ON A SPECIAL POSITION.
REMARK 375      HOH A 587  LIES ON A SPECIAL POSITION.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     THR A     2
REMARK 465     HIS A   281
REMARK 465     HIS A   282
REMARK 465     HIS A   283
REMARK 480
REMARK 480 ZERO OCCUPANCY ATOM
REMARK 480 THE FOLLOWING RESIDUES HAVE ATOMS MODELED WITH ZERO
REMARK 480 OCCUPANCY. THE LOCATION AND PROPERTIES OF THESE ATOMS
REMARK 480 MAY NOT BE RELIABLE. (M=MODEL NUMBER; RES=RESIDUE NAME;
REMARK 480 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 480   M RES C SSEQI ATOMS
REMARK 480     ASP A    9   OD2
REMARK 480     GLN A   23   NE2
REMARK 480     ALA A   70   CB
REMARK 480     GLU A  130   OE1  OE2
REMARK 480     GLY A  143   N    CA
REMARK 480     HIS A  144   CG   ND1  CD2  CE1  NE2
REMARK 480     GLU A  189   OE2
REMARK 480     ASP A  208   CG   OD1  OD2
REMARK 480     LYS A  209   CD   CE   NZ
REMARK 480     LYS A  262   CE   NZ
REMARK 480     GLN A  264   CB   CG   CD   OE1  NE2
REMARK 480     GLU A  277   OE1  OE2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ARG A   8     -122.77     48.58
REMARK 500    CYS A  86      165.75     83.52
REMARK 500    HIS A 144     -114.62     71.96
REMARK 500    SER A 152     -122.75     59.99
REMARK 500    SER A 152     -121.26     57.51
REMARK 500    HIS A 206       47.55   -140.81
REMARK 500
REMARK 500 REMARK: NULL
REMARK 620
REMARK 620 METAL COORDINATION
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 620
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL
REMARK 620                              CA A 301  CA
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 GLN A  36   OE1
REMARK 620 2 GLN A  36   OE1   0.0
REMARK 620 3 ASP A  90   OD1  89.9  89.9
REMARK 620 4 ASP A  90   OD1  89.9  89.9   0.0
REMARK 620 5 HOH A 502   O    89.8  89.8  87.5  87.5
REMARK 620 6 HOH A 502   O    89.8  89.8  87.5  87.5   0.0
REMARK 620 7 HOH A 565   O    90.2  90.2  92.5  92.5 180.0 180.0
REMARK 620 8 HOH A 565   O    90.2  90.2  92.5  92.5 180.0 180.0   0.0
REMARK 620 N                    1     2     3     4     5     6     7
DBREF  9N8R A    1   283  PDB    9N8R     9N8R             1    283
SEQRES   1 A  283  MET THR MET THR LEU LEU TYR ARG ASP MET ASN GLN ALA
SEQRES   2 A  283  GLN LEU ASP ALA ALA TYR ASN ASN THR GLN ALA VAL PRO
SEQRES   3 A  283  ASP PHE PRO GLY ILE TYR ALA ALA LEU GLN ALA ARG SER
SEQRES   4 A  283  ALA SER PHE TYR ALA SER ALA ALA GLY ARG LEU ASN LEU
SEQRES   5 A  283  PRO TYR GLY THR ALA PRO ARG GLN ARG TYR ASP TRP LEU
SEQRES   6 A  283  PRO CYS GLY LYS ALA ASP ALA PRO THR LEU ILE PHE ILE
SEQRES   7 A  283  HIS GLY GLY TYR TRP GLN ASN CYS SER LYS GLU ASP PHE
SEQRES   8 A  283  ALA PHE ILE ALA ALA GLY PRO LEU ALA ALA GLY PHE ASN
SEQRES   9 A  283  ILE VAL LEU ALA GLU TYR THR LEU ALA PRO GLN ALA SER
SEQRES  10 A  283  MET THR GLN ILE VAL SER GLU ILE GLY SER LEU LEU GLU
SEQRES  11 A  283  HIS LEU GLN ALA ASP ALA ASP GLN LEU GLY ILE ALA GLY
SEQRES  12 A  283  HIS LYS VAL VAL LEU SER GLY HIS SER ALA GLY GLY HIS
SEQRES  13 A  283  LEU ALA LEU GLN PHE ARG SER HIS PRO TRP VAL THR ASP
SEQRES  14 A  283  VAL LEU ALA ILE SER ALA LEU VAL ASP LEU GLU PRO ILE
SEQRES  15 A  283  SER LEU SER TRP LEU ASN GLU LYS LEU SER LEU SER GLU
SEQRES  16 A  283  ALA GLU ILE ASP ALA TYR SER PRO LEU TYR HIS ILE ASP
SEQRES  17 A  283  LYS GLY ALA ASN THR TRP VAL ALA VAL GLY ALA ASP GLU
SEQRES  18 A  283  LEU SER GLU LEU VAL ARG GLN SER ASP GLU TYR ALA LYS
SEQRES  19 A  283  GLN ALA LEU ALA ARG GLY GLU SER VAL GLN LEU ILE HIS
SEQRES  20 A  283  VAL PRO GLY CYS THR HIS PHE SER VAL LEU ASP GLU MET
SEQRES  21 A  283  ALA LYS PRO GLN GLY ALA LEU LEU GLN ALA LEU SER SER
SEQRES  22 A  283  ILE ARG LEU GLU HIS HIS HIS HIS HIS HIS
HET     CA  A 301       1
HETNAM      CA CALCIUM ION
FORMUL   2   CA    CA 2+
FORMUL   3  HOH   *193(H2 O)
HELIX    1 AA1 ASN A   11  ASN A   20  1                                  10
HELIX    2 AA2 ASN A   20  VAL A   25  1                                   6
HELIX    3 AA3 ASP A   27  ALA A   46  1                                  20
HELIX    4 AA4 SER A   87  ALA A   92  1                                   6
HELIX    5 AA5 ALA A   95  ALA A  101  1                                   7
HELIX    6 AA6 SER A  117  ALA A  134  1                                  18
HELIX    7 AA7 SER A  152  PHE A  161  1                                  10
HELIX    8 AA8 ASP A  178  SER A  185  1                                   8
HELIX    9 AA9 LEU A  187  SER A  192  1                                   6
HELIX   10 AB1 SER A  194  TYR A  201  1                                   8
HELIX   11 AB2 SER A  202  HIS A  206  5                                   5
HELIX   12 AB3 LEU A  222  ARG A  239  1                                  18
HELIX   13 AB4 PHE A  254  VAL A  256  5                                   3
HELIX   14 AB5 LEU A  257  LYS A  262  1                                   6
HELIX   15 AB6 LEU A  267  HIS A  280  1                                  14
SHEET    1 AA1 8 ARG A  49  PRO A  53  0
SHEET    2 AA1 8 ARG A  61  LEU A  65 -1  O  TYR A  62   N  LEU A  52
SHEET    3 AA1 8 ASN A 104  ALA A 108 -1  O  ILE A 105   N  LEU A  65
SHEET    4 AA1 8 THR A  74  ILE A  78  1  N  PHE A  77   O  VAL A 106
SHEET    5 AA1 8 VAL A 146  HIS A 151  1  O  VAL A 147   N  ILE A  76
SHEET    6 AA1 8 ASP A 169  ILE A 173  1  O  ILE A 173   N  GLY A 150
SHEET    7 AA1 8 ASN A 212  GLY A 218  1  O  TRP A 214   N  ALA A 172
SHEET    8 AA1 8 VAL A 243  VAL A 248  1  O  GLN A 244   N  VAL A 215
LINK         OE1 GLN A  36                CA    CA A 301     1555   1555  2.32
LINK         OE1 GLN A  36                CA    CA A 301     1555   2655  2.32
LINK         OD1 ASP A  90                CA    CA A 301     1555   1555  2.31
LINK         OD1 ASP A  90                CA    CA A 301     1555   2655  2.31
LINK        CA    CA A 301                 O   HOH A 502     1555   1555  2.39
LINK        CA    CA A 301                 O   HOH A 502     1555   2655  2.39
LINK        CA    CA A 301                 O   HOH A 565     1555   1555  2.43
LINK        CA    CA A 301                 O   HOH A 565     1555   2655  2.43
CISPEP   1 ALA A  113    PRO A  114          0         7.46
CRYST1   68.595   75.309   54.155  90.00  90.00  90.00 P 21 21 2     4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.014578  0.000000  0.000000        0.00000
SCALE2      0.000000  0.013279  0.000000        0.00000
SCALE3      0.000000  0.000000  0.018466        0.00000
TER    4258      HIS A 280
MASTER      297    0    1   15    8    0    0    6 2322    1    5   22
END