longtext: 9g73-pdb

content
HEADER    HYDROLASE                               19-JUL-24   9G73
TITLE     CRYSTAL STRUCTURE OF MOUSE CARBOXYLESTERASE 2E (CES2E)
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: PYRETHROID HYDROLASE CES2E;
COMPND   3 CHAIN: A, B;
COMPND   4 SYNONYM: CARBOXYLIC ESTER HYDROLASE,CARBOXYLESTERASE 2E;
COMPND   5 EC: 3.1.1.88,3.1.1.-;
COMPND   6 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;
SOURCE   3 ORGANISM_COMMON: HOUSE MOUSE;
SOURCE   4 ORGANISM_TAXID: 10090;
SOURCE   5 GENE: CES2E, CES5;
SOURCE   6 EXPRESSION_SYSTEM: HOMO SAPIENS;
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 9606;
SOURCE   8 EXPRESSION_SYSTEM_CELL_LINE: HEK
KEYWDS    CARBOXYLESTERASE 2E; PROTEIN STRUCTURE; LIPID METABOLISM; X-RAY
KEYWDS   2 CRYSTALLOGRAPHY; ALPHA/BETA-HYDROLASE FOLD; BIOCHEMISTRY; LIPID
KEYWDS   3 HYDROLYSIS; NON-ALCOHOLIC FATTY LIVER DISEASE; HYDROLASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    H.EISNER,T.SAGMEISTER,L.RAMMER,M.OBERER
REVDAT   1   13-AUG-25 9G73    0
JRNL        AUTH   H.EISNER,L.RAMMER,L.RIEGLER-BERKET,C.RODRIGUEZ GAMEZ,
JRNL        AUTH 2 T.SAGMEISTER,G.CHALHOUB,L.LIESINGER,R.BIRNER-GRUENBERGER,
JRNL        AUTH 3 T.PAVKOV-KELLER,G.HAEMMERLE,G.SCHOISWOHL,M.OBERER
JRNL        TITL   CRYSTAL STRUCTURE OF MOUSE CES2E
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    2.40 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : REFMAC 5.8.0267
REMARK   3   AUTHORS     : MURSHUDOV,SKUBAK,LEBEDEV,PANNU,STEINER,
REMARK   3               : NICHOLLS,WINN,LONG,VAGIN
REMARK   3
REMARK   3    REFINEMENT TARGET : NULL
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.40
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 80.65
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.9
REMARK   3   NUMBER OF REFLECTIONS             : 54111
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : FREE R-VALUE
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL
REMARK   3   R VALUE     (WORKING + TEST SET) : NULL
REMARK   3   R VALUE            (WORKING SET) : 0.176
REMARK   3   FREE R VALUE                     : 0.214
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.023
REMARK   3   FREE R VALUE TEST SET COUNT      : 2718
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.40
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.46
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 3789
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 99.97
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2530
REMARK   3   BIN FREE R VALUE SET COUNT          : 179
REMARK   3   BIN FREE R VALUE                    : 0.2800
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 8154
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 249
REMARK   3   SOLVENT ATOMS            : 698
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 36.31
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : -1.46100
REMARK   3    B22 (A**2) : -1.11100
REMARK   3    B33 (A**2) : 2.57200
REMARK   3    B12 (A**2) : 0.00000
REMARK   3    B13 (A**2) : 0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.
REMARK   3   ESU BASED ON R VALUE                            (A): 0.337
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.220
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.176
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 7.997
REMARK   3
REMARK   3 CORRELATION COEFFICIENTS.
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.960
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.935
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  8680 ; 0.009 ; 0.013
REMARK   3   BOND LENGTHS OTHERS               (A):  7983 ; 0.001 ; 0.015
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 11803 ; 1.543 ; 1.665
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 18474 ; 1.301 ; 1.597
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  1047 ; 7.041 ; 5.000
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   421 ;32.213 ;22.922
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1385 ;13.344 ;15.000
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    40 ;19.211 ;15.000
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1122 ; 0.078 ; 0.200
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  9636 ; 0.007 ; 0.020
REMARK   3   GENERAL PLANES OTHERS             (A):  1900 ; 0.001 ; 0.020
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  1681 ; 0.194 ; 0.200
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):   121 ; 0.220 ; 0.200
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  4105 ; 0.160 ; 0.200
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   491 ; 0.178 ; 0.200
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  4183 ; 3.018 ; 3.450
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  4182 ; 3.007 ; 3.449
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  5230 ; 4.875 ; 5.159
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  5231 ; 4.875 ; 5.160
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  4497 ; 4.134 ; 4.176
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  4438 ; 4.130 ; 4.155
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  6570 ; 6.499 ; 6.026
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  6481 ; 6.504 ; 5.991
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL
REMARK   3
REMARK   3  NCS RESTRAINTS STATISTICS
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : 1
REMARK   3
REMARK   3  NCS GROUP NUMBER               : 1
REMARK   3     CHAIN NAMES                    : A B
REMARK   3     NUMBER OF COMPONENTS NCS GROUP : 2
REMARK   3       COMPONENT C  SSSEQI  TO  C   SSSEQI   CODE
REMARK   3           1     A     28       A     550    NULL
REMARK   3           2     B     28       B     550    NULL
REMARK   3                   GROUP CHAIN        COUNT   RMS     WEIGHT
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED : MASK BULK SOLVENT
REMARK   3   PARAMETERS FOR MASK CALCULATION
REMARK   3   VDW PROBE RADIUS   : 1.20
REMARK   3   ION PROBE RADIUS   : 0.80
REMARK   3   SHRINKAGE RADIUS   : 0.80
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THEIR
REMARK   3  RIDING POSITIONS
REMARK   4
REMARK   4 9G73 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 19-JUL-24.
REMARK 100 THE DEPOSITION ID IS D_1292133137.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 30-JAN-22
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : NULL
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : ESRF
REMARK 200  BEAMLINE                       : ID23-2
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.873128
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER2 X 9M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 54113
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.400
REMARK 200  RESOLUTION RANGE LOW       (A) : 80.646
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9
REMARK 200  DATA REDUNDANCY                : 2.000
REMARK 200  R MERGE                    (I) : 0.07550
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 9.3800
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.40
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.49
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 0.41570
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 2.190
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 54.84
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.72
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: PROTEIN STOCK SOLUTION IN 20 MM
REMARK 280  TRIS/HCL, PH 7.4, AND 150 MM NACL; SHOTGUN (SG1) SCREEN: E1 (2.0
REMARK 280  M AMMONIUM SULFATE AND 0.1 M BIS-TRIS BUFFER PH 5.5) WITH
REMARK 280  PROTEIN END CONCENTRATION OF 2.89 MG/ML IN A 0.5 UL DROP, VAPOR
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 293.15K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X,-Y,Z+1/2
REMARK 290       3555   -X,Y,-Z+1/2
REMARK 290       4555   X,-Y,-Z
REMARK 290       5555   X+1/2,Y+1/2,Z
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2
REMARK 290       8555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       74.71600
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       74.71600
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       63.46550
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       72.62150
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       63.46550
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       72.62150
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       74.71600
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       63.46550
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       72.62150
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       74.71600
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       63.46550
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       72.62150
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 7420 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 43490 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -164.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     PRO A     2
REMARK 465     LEU A     3
REMARK 465     TYR A     4
REMARK 465     LYS A     5
REMARK 465     LEU A     6
REMARK 465     LEU A     7
REMARK 465     GLY A     8
REMARK 465     TRP A     9
REMARK 465     LEU A    10
REMARK 465     ASN A    11
REMARK 465     ALA A    12
REMARK 465     VAL A    13
REMARK 465     ALA A    14
REMARK 465     CYS A    15
REMARK 465     GLY A    16
REMARK 465     VAL A    17
REMARK 465     LEU A    18
REMARK 465     LEU A    19
REMARK 465     LEU A    20
REMARK 465     VAL A    21
REMARK 465     LEU A    22
REMARK 465     HIS A    23
REMARK 465     VAL A    24
REMARK 465     GLN A    25
REMARK 465     GLY A    26
REMARK 465     GLN A    27
REMARK 465     LEU A   101
REMARK 465     MET A   102
REMARK 465     GLY A   103
REMARK 465     SER A   104
REMARK 465     GLU A   105
REMARK 465     ASP A   106
REMARK 465     SER A   552
REMARK 465     GLN A   553
REMARK 465     GLU A   554
REMARK 465     ARG A   555
REMARK 465     HIS A   556
REMARK 465     LYS A   557
REMARK 465     GLU A   558
REMARK 465     LEU A   559
REMARK 465     HIS A   560
REMARK 465     HIS A   561
REMARK 465     HIS A   562
REMARK 465     HIS A   563
REMARK 465     HIS A   564
REMARK 465     HIS A   565
REMARK 465     MET B     1
REMARK 465     PRO B     2
REMARK 465     LEU B     3
REMARK 465     TYR B     4
REMARK 465     LYS B     5
REMARK 465     LEU B     6
REMARK 465     LEU B     7
REMARK 465     GLY B     8
REMARK 465     TRP B     9
REMARK 465     LEU B    10
REMARK 465     ASN B    11
REMARK 465     ALA B    12
REMARK 465     VAL B    13
REMARK 465     ALA B    14
REMARK 465     CYS B    15
REMARK 465     GLY B    16
REMARK 465     VAL B    17
REMARK 465     LEU B    18
REMARK 465     LEU B    19
REMARK 465     LEU B    20
REMARK 465     VAL B    21
REMARK 465     LEU B    22
REMARK 465     HIS B    23
REMARK 465     VAL B    24
REMARK 465     GLN B    25
REMARK 465     GLY B    26
REMARK 465     LEU B   101
REMARK 465     MET B   102
REMARK 465     GLY B   103
REMARK 465     SER B   104
REMARK 465     GLU B   105
REMARK 465     GLU B   554
REMARK 465     ARG B   555
REMARK 465     HIS B   556
REMARK 465     LYS B   557
REMARK 465     GLU B   558
REMARK 465     LEU B   559
REMARK 465     HIS B   560
REMARK 465     HIS B   561
REMARK 465     HIS B   562
REMARK 465     HIS B   563
REMARK 465     HIS B   564
REMARK 465     HIS B   565
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    HOH A   954     O    HOH A  1017              2.09
REMARK 500   O    HOH A   701     O    HOH A   915              2.10
REMARK 500   O    HOH A   948     O    HOH A   955              2.11
REMARK 500   O    HOH B   797     O    HOH B   872              2.12
REMARK 500   OD2  ASP A   220     O    HOH A   701              2.13
REMARK 500   O    HOH A   994     O    HOH A  1038              2.13
REMARK 500   ND1  HIS A   425     O    HOH A   702              2.16
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASP A  50       19.46     52.38
REMARK 500    ALA A 192       82.39   -158.80
REMARK 500    SER A 228     -115.66     58.92
REMARK 500    GLU A 317      -70.49   -128.73
REMARK 500    ASP A 344       59.45   -142.16
REMARK 500    TRP A 348      -64.09   -144.92
REMARK 500    PHE A 416      -52.43   -130.36
REMARK 500    LYS A 454     -130.58    -91.34
REMARK 500    ASN A 471       25.31     47.66
REMARK 500    ASP A 512     -153.31   -126.29
REMARK 500    LYS A 540      -57.01   -128.61
REMARK 500    ASP B  99       38.44    -95.08
REMARK 500    ILE B 113       34.12    -99.00
REMARK 500    ALA B 192       81.16   -157.34
REMARK 500    SER B 228     -117.15     58.45
REMARK 500    GLU B 317      -69.34   -127.04
REMARK 500    TRP B 348      -63.92   -145.86
REMARK 500    PHE B 416      -52.61   -130.23
REMARK 500    LYS B 454     -128.59    -90.69
REMARK 500    ASP B 512     -155.54   -127.52
REMARK 500    LYS B 540      -56.05   -128.03
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A1068        DISTANCE =  6.08 ANGSTROMS
REMARK 525    HOH B1026        DISTANCE =  5.98 ANGSTROMS
REMARK 525    HOH B1027        DISTANCE =  6.20 ANGSTROMS
REMARK 525    HOH B1028        DISTANCE =  6.92 ANGSTROMS
DBREF  9G73 A    1   559  UNP    Q8BK48   EST2E_MOUSE      1    559
DBREF  9G73 B    1   559  UNP    Q8BK48   EST2E_MOUSE      1    559
SEQADV 9G73 HIS A  560  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS A  561  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS A  562  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS A  563  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS A  564  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS A  565  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS B  560  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS B  561  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS B  562  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS B  563  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS B  564  UNP  Q8BK48              EXPRESSION TAG
SEQADV 9G73 HIS B  565  UNP  Q8BK48              EXPRESSION TAG
SEQRES   1 A  565  MET PRO LEU TYR LYS LEU LEU GLY TRP LEU ASN ALA VAL
SEQRES   2 A  565  ALA CYS GLY VAL LEU LEU LEU VAL LEU HIS VAL GLN GLY
SEQRES   3 A  565  GLN ASP SER ALA SER PRO ILE ARG ASN THR HIS THR GLY
SEQRES   4 A  565  GLN VAL ARG GLY SER LEU VAL HIS VAL LYS ASP THR ASP
SEQRES   5 A  565  ILE ALA VAL HIS THR PHE LEU GLY ILE PRO PHE ALA LYS
SEQRES   6 A  565  PRO PRO VAL GLY PRO LEU ARG PHE ALA PRO PRO GLU ALA
SEQRES   7 A  565  PRO GLU PRO TRP SER GLY VAL ARG ASP GLY THR SER HIS
SEQRES   8 A  565  PRO ASN MET CYS LEU GLN ASN ASP ASN LEU MET GLY SER
SEQRES   9 A  565  GLU ASP LEU LYS MET MET ASN LEU ILE LEU PRO PRO ILE
SEQRES  10 A  565  SER MET SER GLU ASP CYS LEU TYR LEU ASN ILE TYR VAL
SEQRES  11 A  565  PRO ALA HIS ALA HIS GLU GLY SER ASN LEU PRO VAL MET
SEQRES  12 A  565  VAL TRP ILE HIS GLY GLY ALA LEU THR VAL GLY MET ALA
SEQRES  13 A  565  SER MET TYR ASP GLY SER MET LEU ALA ALA THR GLU ASP
SEQRES  14 A  565  VAL VAL VAL VAL ALA ILE GLN TYR ARG LEU GLY VAL LEU
SEQRES  15 A  565  GLY PHE PHE SER THR GLY ASP GLN HIS ALA LYS GLY ASN
SEQRES  16 A  565  TRP GLY TYR LEU ASP GLN VAL ALA ALA LEU ARG TRP VAL
SEQRES  17 A  565  GLN GLN ASN ILE VAL HIS PHE GLY GLY ASN PRO ASP ARG
SEQRES  18 A  565  VAL THR ILE PHE GLY GLU SER ALA GLY GLY THR SER VAL
SEQRES  19 A  565  SER SER HIS VAL VAL SER PRO MET SER GLN GLY LEU PHE
SEQRES  20 A  565  HIS GLY ALA ILE MET GLU SER GLY VAL ALA VAL LEU PRO
SEQRES  21 A  565  ASP LEU ILE SER SER SER SER GLU MET VAL HIS ARG ILE
SEQRES  22 A  565  VAL ALA ASN LEU SER GLY CYS ALA ALA VAL ASN SER GLU
SEQRES  23 A  565  THR LEU MET CYS CYS LEU ARG GLY LYS ASN GLU ALA GLU
SEQRES  24 A  565  MET LEU ALA ILE ASN LYS VAL PHE LYS ILE ILE PRO GLY
SEQRES  25 A  565  VAL VAL ASP GLY GLU PHE LEU PRO LYS HIS PRO GLN GLU
SEQRES  26 A  565  LEU MET ALA SER LYS ASP PHE HIS PRO VAL PRO SER ILE
SEQRES  27 A  565  ILE GLY ILE ASN ASN ASP GLU TYR GLY TRP ILE LEU PRO
SEQRES  28 A  565  THR ILE MET ASP PRO ALA GLN LYS ILE GLU GLU ILE THR
SEQRES  29 A  565  ARG LYS THR LEU PRO ALA VAL LEU LYS SER THR ALA LEU
SEQRES  30 A  565  LYS MET MET LEU PRO PRO GLU CYS GLY ASP LEU LEU MET
SEQRES  31 A  565  GLU GLU TYR MET GLY ASP THR GLU ASP PRO GLU THR LEU
SEQRES  32 A  565  GLN ALA GLN PHE ARG GLU MET LYS GLY ASP PHE MET PHE
SEQRES  33 A  565  VAL ILE PRO ALA LEU GLN VAL ALA HIS PHE GLN ARG SER
SEQRES  34 A  565  HIS ALA PRO VAL TYR PHE TYR GLU PHE GLN HIS ARG PRO
SEQRES  35 A  565  SER PHE PHE LYS ASP PHE ARG PRO PRO TYR VAL LYS ALA
SEQRES  36 A  565  ASP HIS GLY ASP GLU ILE PHE LEU VAL PHE GLY TYR GLN
SEQRES  37 A  565  PHE GLY ASN ILE LYS LEU PRO TYR THR GLU GLU GLU GLU
SEQRES  38 A  565  GLN LEU SER ARG ARG ILE MET LYS TYR TRP ALA ASN PHE
SEQRES  39 A  565  ALA ARG HIS GLY ASN PRO ASN SER GLU GLY LEU PRO TYR
SEQRES  40 A  565  TRP PRO VAL MET ASP HIS ASP GLU GLN TYR LEU GLN LEU
SEQRES  41 A  565  ASP ILE GLN PRO SER VAL GLY ARG ALA LEU LYS ALA ARG
SEQRES  42 A  565  ARG LEU GLN PHE TRP THR LYS THR LEU PRO GLN LYS ILE
SEQRES  43 A  565  GLN GLU LEU LYS GLY SER GLN GLU ARG HIS LYS GLU LEU
SEQRES  44 A  565  HIS HIS HIS HIS HIS HIS
SEQRES   1 B  565  MET PRO LEU TYR LYS LEU LEU GLY TRP LEU ASN ALA VAL
SEQRES   2 B  565  ALA CYS GLY VAL LEU LEU LEU VAL LEU HIS VAL GLN GLY
SEQRES   3 B  565  GLN ASP SER ALA SER PRO ILE ARG ASN THR HIS THR GLY
SEQRES   4 B  565  GLN VAL ARG GLY SER LEU VAL HIS VAL LYS ASP THR ASP
SEQRES   5 B  565  ILE ALA VAL HIS THR PHE LEU GLY ILE PRO PHE ALA LYS
SEQRES   6 B  565  PRO PRO VAL GLY PRO LEU ARG PHE ALA PRO PRO GLU ALA
SEQRES   7 B  565  PRO GLU PRO TRP SER GLY VAL ARG ASP GLY THR SER HIS
SEQRES   8 B  565  PRO ASN MET CYS LEU GLN ASN ASP ASN LEU MET GLY SER
SEQRES   9 B  565  GLU ASP LEU LYS MET MET ASN LEU ILE LEU PRO PRO ILE
SEQRES  10 B  565  SER MET SER GLU ASP CYS LEU TYR LEU ASN ILE TYR VAL
SEQRES  11 B  565  PRO ALA HIS ALA HIS GLU GLY SER ASN LEU PRO VAL MET
SEQRES  12 B  565  VAL TRP ILE HIS GLY GLY ALA LEU THR VAL GLY MET ALA
SEQRES  13 B  565  SER MET TYR ASP GLY SER MET LEU ALA ALA THR GLU ASP
SEQRES  14 B  565  VAL VAL VAL VAL ALA ILE GLN TYR ARG LEU GLY VAL LEU
SEQRES  15 B  565  GLY PHE PHE SER THR GLY ASP GLN HIS ALA LYS GLY ASN
SEQRES  16 B  565  TRP GLY TYR LEU ASP GLN VAL ALA ALA LEU ARG TRP VAL
SEQRES  17 B  565  GLN GLN ASN ILE VAL HIS PHE GLY GLY ASN PRO ASP ARG
SEQRES  18 B  565  VAL THR ILE PHE GLY GLU SER ALA GLY GLY THR SER VAL
SEQRES  19 B  565  SER SER HIS VAL VAL SER PRO MET SER GLN GLY LEU PHE
SEQRES  20 B  565  HIS GLY ALA ILE MET GLU SER GLY VAL ALA VAL LEU PRO
SEQRES  21 B  565  ASP LEU ILE SER SER SER SER GLU MET VAL HIS ARG ILE
SEQRES  22 B  565  VAL ALA ASN LEU SER GLY CYS ALA ALA VAL ASN SER GLU
SEQRES  23 B  565  THR LEU MET CYS CYS LEU ARG GLY LYS ASN GLU ALA GLU
SEQRES  24 B  565  MET LEU ALA ILE ASN LYS VAL PHE LYS ILE ILE PRO GLY
SEQRES  25 B  565  VAL VAL ASP GLY GLU PHE LEU PRO LYS HIS PRO GLN GLU
SEQRES  26 B  565  LEU MET ALA SER LYS ASP PHE HIS PRO VAL PRO SER ILE
SEQRES  27 B  565  ILE GLY ILE ASN ASN ASP GLU TYR GLY TRP ILE LEU PRO
SEQRES  28 B  565  THR ILE MET ASP PRO ALA GLN LYS ILE GLU GLU ILE THR
SEQRES  29 B  565  ARG LYS THR LEU PRO ALA VAL LEU LYS SER THR ALA LEU
SEQRES  30 B  565  LYS MET MET LEU PRO PRO GLU CYS GLY ASP LEU LEU MET
SEQRES  31 B  565  GLU GLU TYR MET GLY ASP THR GLU ASP PRO GLU THR LEU
SEQRES  32 B  565  GLN ALA GLN PHE ARG GLU MET LYS GLY ASP PHE MET PHE
SEQRES  33 B  565  VAL ILE PRO ALA LEU GLN VAL ALA HIS PHE GLN ARG SER
SEQRES  34 B  565  HIS ALA PRO VAL TYR PHE TYR GLU PHE GLN HIS ARG PRO
SEQRES  35 B  565  SER PHE PHE LYS ASP PHE ARG PRO PRO TYR VAL LYS ALA
SEQRES  36 B  565  ASP HIS GLY ASP GLU ILE PHE LEU VAL PHE GLY TYR GLN
SEQRES  37 B  565  PHE GLY ASN ILE LYS LEU PRO TYR THR GLU GLU GLU GLU
SEQRES  38 B  565  GLN LEU SER ARG ARG ILE MET LYS TYR TRP ALA ASN PHE
SEQRES  39 B  565  ALA ARG HIS GLY ASN PRO ASN SER GLU GLY LEU PRO TYR
SEQRES  40 B  565  TRP PRO VAL MET ASP HIS ASP GLU GLN TYR LEU GLN LEU
SEQRES  41 B  565  ASP ILE GLN PRO SER VAL GLY ARG ALA LEU LYS ALA ARG
SEQRES  42 B  565  ARG LEU GLN PHE TRP THR LYS THR LEU PRO GLN LYS ILE
SEQRES  43 B  565  GLN GLU LEU LYS GLY SER GLN GLU ARG HIS LYS GLU LEU
SEQRES  44 B  565  HIS HIS HIS HIS HIS HIS
HET    NAG  C   1      27
HET    NAG  C   2      25
HET    BMA  C   3      19
HET    MAN  C   4      18
HET    MAN  C   5      18
HET    FUC  C   6      18
HET    NAG  D   1      27
HET    NAG  D   2      25
HET    BMA  D   3      19
HET    MAN  D   4      19
HET    NAG  D   5      24
HET    MAN  D   6      19
HET    NAG  D   7      24
HET    FUC  D   8      18
HET    SO4  A 601       5
HET    SO4  A 602       5
HET    SO4  A 603       5
HET    SO4  A 604       5
HET    SO4  A 605       5
HET    SO4  A 606       5
HET    SO4  A 607       5
HET     CL  A 608       1
HET     CL  A 609       1
HET    SO4  B 601       5
HET    SO4  B 602       5
HET    SO4  B 603       5
HET    SO4  B 604       5
HET    SO4  B 605       5
HET    SO4  B 606       5
HET    SO4  B 607       5
HET    SO4  B 608       5
HET     CL  B 609       1
HET     CL  B 610       1
HETNAM     NAG 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSE
HETNAM     BMA BETA-D-MANNOPYRANOSE
HETNAM     MAN ALPHA-D-MANNOPYRANOSE
HETNAM     FUC ALPHA-L-FUCOPYRANOSE
HETNAM     SO4 SULFATE ION
HETNAM      CL CHLORIDE ION
HETSYN     NAG N-ACETYL-BETA-D-GLUCOSAMINE; 2-ACETAMIDO-2-DEOXY-BETA-
HETSYN   2 NAG  D-GLUCOSE; 2-ACETAMIDO-2-DEOXY-D-GLUCOSE; 2-ACETAMIDO-
HETSYN   3 NAG  2-DEOXY-GLUCOSE; N-ACETYL-D-GLUCOSAMINE
HETSYN     BMA BETA-D-MANNOSE; D-MANNOSE; MANNOSE
HETSYN     MAN ALPHA-D-MANNOSE; D-MANNOSE; MANNOSE
HETSYN     FUC ALPHA-L-FUCOSE; 6-DEOXY-ALPHA-L-GALACTOPYRANOSE; L-
HETSYN   2 FUC  FUCOSE; FUCOSE
FORMUL   3  NAG    6(C8 H15 N O6)
FORMUL   3  BMA    2(C6 H12 O6)
FORMUL   3  MAN    4(C6 H12 O6)
FORMUL   3  FUC    2(C6 H12 O5)
FORMUL   5  SO4    15(O4 S 2-)
FORMUL  12   CL    4(CL 1-)
FORMUL  24  HOH   *698(H2 O)
HELIX    1 AA1 VAL A   68  ARG A   72  5                                   5
HELIX    2 AA2 LEU A  107  LEU A  114  5                                   8
HELIX    3 AA3 MET A  155  TYR A  159  5                                   5
HELIX    4 AA4 GLY A  161  ASP A  169  1                                   9
HELIX    5 AA5 LEU A  179  PHE A  185  1                                   7
HELIX    6 AA6 ASN A  195  ILE A  212  1                                  18
HELIX    7 AA7 VAL A  213  PHE A  215  5                                   3
HELIX    8 AA8 SER A  228  SER A  240  1                                  13
HELIX    9 AA9 PRO A  241  GLN A  244  5                                   4
HELIX   10 AB1 LEU A  259  ASP A  261  5                                   3
HELIX   11 AB2 SER A  267  SER A  278  1                                  12
HELIX   12 AB3 ASN A  284  LYS A  295  1                                  12
HELIX   13 AB4 ASN A  296  LYS A  305  1                                  10
HELIX   14 AB5 HIS A  322  SER A  329  1                                   8
HELIX   15 AB6 TRP A  348  ASP A  355  1                                   8
HELIX   16 AB7 GLN A  358  ILE A  363  5                                   6
HELIX   17 AB8 THR A  367  MET A  379  1                                  13
HELIX   18 AB9 PRO A  382  GLU A  384  5                                   3
HELIX   19 AC1 CYS A  385  MET A  394  1                                  10
HELIX   20 AC2 ASP A  399  PHE A  416  1                                  18
HELIX   21 AC3 PHE A  416  SER A  429  1                                  14
HELIX   22 AC4 PRO A  442  ASP A  447  1                                   6
HELIX   23 AC5 GLU A  460  PHE A  465  1                                   6
HELIX   24 AC6 THR A  477  GLY A  498  1                                  22
HELIX   25 AC7 LYS A  531  LYS A  540  1                                  10
HELIX   26 AC8 LYS A  540  LYS A  550  1                                  11
HELIX   27 AC9 VAL B   68  ARG B   72  5                                   5
HELIX   28 AD1 LEU B  107  ILE B  113  1                                   7
HELIX   29 AD2 MET B  155  TYR B  159  5                                   5
HELIX   30 AD3 GLY B  161  ASP B  169  1                                   9
HELIX   31 AD4 LEU B  179  PHE B  185  1                                   7
HELIX   32 AD5 ASN B  195  ILE B  212  1                                  18
HELIX   33 AD6 VAL B  213  PHE B  215  5                                   3
HELIX   34 AD7 SER B  228  SER B  240  1                                  13
HELIX   35 AD8 PRO B  241  GLN B  244  5                                   4
HELIX   36 AD9 LEU B  259  ASP B  261  5                                   3
HELIX   37 AE1 SER B  267  SER B  278  1                                  12
HELIX   38 AE2 ASN B  284  LYS B  295  1                                  12
HELIX   39 AE3 ASN B  296  LYS B  305  1                                  10
HELIX   40 AE4 HIS B  322  SER B  329  1                                   8
HELIX   41 AE5 TRP B  348  ASP B  355  1                                   8
HELIX   42 AE6 GLN B  358  ILE B  363  5                                   6
HELIX   43 AE7 THR B  367  MET B  379  1                                  13
HELIX   44 AE8 PRO B  382  GLU B  384  5                                   3
HELIX   45 AE9 CYS B  385  MET B  394  1                                  10
HELIX   46 AF1 ASP B  399  PHE B  416  1                                  18
HELIX   47 AF2 PHE B  416  ARG B  428  1                                  13
HELIX   48 AF3 PRO B  442  ASP B  447  1                                   6
HELIX   49 AF4 GLU B  460  PHE B  465  1                                   6
HELIX   50 AF5 THR B  477  GLY B  498  1                                  22
HELIX   51 AF6 LYS B  531  LYS B  540  1                                  10
HELIX   52 AF7 LYS B  540  LYS B  550  1                                  11
HELIX   53 AF8 GLY B  551  GLN B  553  5                                   3
SHEET    1 AA1 3 ILE A  33  ARG A  34  0
SHEET    2 AA1 3 GLN A  40  ARG A  42 -1  O  VAL A  41   N  ARG A  34
SHEET    3 AA1 3 VAL A  85  ASP A  87  1  O  ARG A  86   N  GLN A  40
SHEET    1 AA211 SER A  44  HIS A  47  0
SHEET    2 AA211 ALA A  54  PRO A  62 -1  O  VAL A  55   N  VAL A  46
SHEET    3 AA211 TYR A 125  PRO A 131 -1  O  VAL A 130   N  HIS A  56
SHEET    4 AA211 VAL A 171  ILE A 175 -1  O  VAL A 172   N  TYR A 129
SHEET    5 AA211 LEU A 140  ILE A 146  1  N  MET A 143   O  VAL A 171
SHEET    6 AA211 GLY A 217  GLU A 227  1  O  THR A 223   N  VAL A 144
SHEET    7 AA211 GLY A 249  GLU A 253  1  O  GLU A 253   N  GLY A 226
SHEET    8 AA211 SER A 337  ASN A 342  1  O  ILE A 338   N  MET A 252
SHEET    9 AA211 VAL A 433  PHE A 438  1  O  PHE A 438   N  ILE A 341
SHEET   10 AA211 GLN A 516  LEU A 520  1  O  LEU A 518   N  GLU A 437
SHEET   11 AA211 SER A 525  ARG A 528 -1  O  SER A 525   N  GLN A 519
SHEET    1 AA3 2 ILE A 263  SER A 264  0
SHEET    2 AA3 2 GLY A 312  VAL A 313  1  O  GLY A 312   N  SER A 264
SHEET    1 AA4 2 GLN A 468  PHE A 469  0
SHEET    2 AA4 2 ILE A 472  LYS A 473 -1  O  ILE A 472   N  PHE A 469
SHEET    1 AA5 3 ILE B  33  ARG B  34  0
SHEET    2 AA5 3 GLN B  40  ARG B  42 -1  O  VAL B  41   N  ARG B  34
SHEET    3 AA5 3 VAL B  85  ASP B  87  1  O  ARG B  86   N  GLN B  40
SHEET    1 AA611 SER B  44  HIS B  47  0
SHEET    2 AA611 ALA B  54  PRO B  62 -1  O  VAL B  55   N  VAL B  46
SHEET    3 AA611 TYR B 125  PRO B 131 -1  O  VAL B 130   N  HIS B  56
SHEET    4 AA611 VAL B 171  ILE B 175 -1  O  ALA B 174   N  ASN B 127
SHEET    5 AA611 LEU B 140  ILE B 146  1  N  MET B 143   O  VAL B 171
SHEET    6 AA611 GLY B 217  GLU B 227  1  O  THR B 223   N  VAL B 144
SHEET    7 AA611 GLY B 249  GLU B 253  1  O  GLU B 253   N  GLY B 226
SHEET    8 AA611 SER B 337  ASN B 342  1  O  ILE B 338   N  MET B 252
SHEET    9 AA611 VAL B 433  PHE B 438  1  O  PHE B 438   N  ILE B 341
SHEET   10 AA611 GLN B 516  LEU B 520  1  O  LEU B 518   N  GLU B 437
SHEET   11 AA611 SER B 525  ARG B 528 -1  O  SER B 525   N  GLN B 519
SHEET    1 AA7 2 ILE B 263  SER B 264  0
SHEET    2 AA7 2 GLY B 312  VAL B 313  1  O  GLY B 312   N  SER B 264
SHEET    1 AA8 2 GLN B 468  PHE B 469  0
SHEET    2 AA8 2 ILE B 472  LYS B 473 -1  O  ILE B 472   N  PHE B 469
SSBOND   1 CYS A   95    CYS A  123                          1555   1555  1.99
SSBOND   2 CYS A  280    CYS A  291                          1555   1555  2.09
SSBOND   3 CYS B   95    CYS B  123                          1555   1555  2.02
SSBOND   4 CYS B  280    CYS B  291                          1555   1555  2.07
LINK         ND2 ASN A 276                 C1  NAG C   1     1555   1555  1.43
LINK         ND2 ASN B 276                 C1  NAG D   1     1555   1555  1.43
LINK         O4  NAG C   1                 C1  NAG C   2     1555   1555  1.45
LINK         O6  NAG C   1                 C1  FUC C   6     1555   1555  1.45
LINK         O4  NAG C   2                 C1  BMA C   3     1555   1555  1.45
LINK         O3  BMA C   3                 C1  MAN C   4     1555   1555  1.47
LINK         O6  BMA C   3                 C1  MAN C   5     1555   1555  1.46
LINK         O4  NAG D   1                 C1  NAG D   2     1555   1555  1.46
LINK         O6  NAG D   1                 C1  FUC D   8     1555   1555  1.45
LINK         O4  NAG D   2                 C1  BMA D   3     1555   1555  1.45
LINK         O3  BMA D   3                 C1  MAN D   4     1555   1555  1.47
LINK         O6  BMA D   3                 C1  MAN D   6     1555   1555  1.45
LINK         O2  MAN D   4                 C1  NAG D   5     1555   1555  1.48
LINK         O2  MAN D   6                 C1  NAG D   7     1555   1555  1.48
CRYST1  126.931  145.243  149.432  90.00  90.00  90.00 C 2 2 21     16
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.007878  0.000000  0.000000        0.00000
SCALE2      0.000000  0.006885  0.000000        0.00000
SCALE3      0.000000  0.000000  0.006692        0.00000
TER    7992      GLY A 551
TER   16038      GLN B 553
MASTER      433    0   33   53   36    0    0    6 9101    2  385   88
END