longtext: 9lf7-pdb

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HEADER    HYDROLASE                               08-JAN-25   9LF7
TITLE     A PAE-HYDROLYSE POC14
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: POC14_A;
COMPND   3 CHAIN: A, B;
COMPND   4 EC: 3.1.1.1;
COMPND   5 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: ERYTHROBACTER CRYPTUS DSM 12079;
SOURCE   3 ORGANISM_TAXID: 1122970;
SOURCE   4 STRAIN: DSM 12079;
SOURCE   5 GENE: POC14;
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008
KEYWDS    ESTERASE, PAE-HYDROLYZING, HYDROLYSIS, ALPHA/BEITA HYDROLYSE,
KEYWDS   2 HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    Z.RONG,Y.H.WU
REVDAT   1   19-FEB-25 9LF7    0
JRNL        AUTH   Z.RONG,L.G.HONG,Y.Y.HUO,D.Q.ZHENG,X.W.XU,J.X.LI,Y.H.WU
JRNL        TITL   STRUCTURE OF A PAE-HYDROLYZING EATERSE
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.80 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX 1.16_3549
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : GEOSTD + MONOMER LIBRARY + CDL V1.2
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.80
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 29.13
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : NULL
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8
REMARK   3   NUMBER OF REFLECTIONS             : 80299
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : NULL
REMARK   3   R VALUE            (WORKING SET) : 0.154
REMARK   3   FREE R VALUE                     : 0.178
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : NULL
REMARK   3   FREE R VALUE TEST SET COUNT      : NULL
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1  1.8640 -  1.8000    0.98        0     0  0.1641 0.2141
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : NULL
REMARK   3   SOLVENT RADIUS     : NULL
REMARK   3   SHRINKAGE RADIUS   : NULL
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : NULL
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : NULL
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 11.60
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 11.60
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.006           4784
REMARK   3   ANGLE     :  0.941           6528
REMARK   3   CHIRALITY :  0.054            751
REMARK   3   PLANARITY :  0.015            860
REMARK   3   DIHEDRAL  : 19.321           2914
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 9LF7 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBC ON 10-JAN-25.
REMARK 100 THE DEPOSITION ID IS D_1300055445.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 24-MAY-18
REMARK 200  TEMPERATURE           (KELVIN) : 193.15
REMARK 200  PH                             : 7.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : NFPSS
REMARK 200  BEAMLINE                       : BL19U1
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97891
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 80299
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.800
REMARK 200  RESOLUTION RANGE LOW       (A) : 29.210
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8
REMARK 200  DATA REDUNDANCY                : 4.800
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 16.9000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.80
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.86
REMARK 200  COMPLETENESS FOR SHELL     (%) : 98.1
REMARK 200  DATA REDUNDANCY IN SHELL       : 1.90
REMARK 200  R MERGE FOR SHELL          (I) : 0.01000
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: MAD
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 58.98
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.00
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M HEPES SODIUM, 2% V/V
REMARK 280  POLYETHYLENE GLYCOL 400, 2.0 M AMMONIUM SULFATE, PH 7.5,
REMARK 280  EVAPORATION, TEMPERATURE 291.15K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -X+1/2,-Y,Z+1/2
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       34.32850
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       69.79600
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       44.80150
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       69.79600
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       34.32850
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       44.80150
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 3290 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 22250 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: 6.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A     1
REMARK 465     ARG A     2
REMARK 465     LEU A     3
REMARK 465     ALA A     4
REMARK 465     LYS A     5
REMARK 465     LEU A     6
REMARK 465     PRO A     7
REMARK 465     ALA A     8
REMARK 465     LEU A     9
REMARK 465     ALA A    10
REMARK 465     TYR A    11
REMARK 465     ARG A    12
REMARK 465     SER A    13
REMARK 465     ALA A    14
REMARK 465     LYS A    15
REMARK 465     ASP A    16
REMARK 465     THR A    17
REMARK 465     GLY A    18
REMARK 465     GLU A    19
REMARK 465     SER A    20
REMARK 465     MET A    21
REMARK 465     THR A    22
REMARK 465     ASP A    23
REMARK 465     MET B     1
REMARK 465     ARG B     2
REMARK 465     LEU B     3
REMARK 465     ALA B     4
REMARK 465     LYS B     5
REMARK 465     LEU B     6
REMARK 465     PRO B     7
REMARK 465     ALA B     8
REMARK 465     LEU B     9
REMARK 465     ALA B    10
REMARK 465     TYR B    11
REMARK 465     ARG B    12
REMARK 465     SER B    13
REMARK 465     ALA B    14
REMARK 465     LYS B    15
REMARK 465     ASP B    16
REMARK 465     THR B    17
REMARK 465     GLY B    18
REMARK 465     GLU B    19
REMARK 465     SER B    20
REMARK 465     MET B    21
REMARK 465     THR B    22
REMARK 465     ASP B    23
REMARK 465     ALA B    99
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   OG   SER B   183     N4   MES B   401              1.31
REMARK 500   OG   SER B   183     C3   MES B   401              2.11
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ARG A  98       81.26   -154.05
REMARK 500    ASP A 117     -162.10   -166.03
REMARK 500    SER A 183     -117.75     67.44
REMARK 500    PHE A 212       63.03     24.57
REMARK 500    LEU A 232      -63.60     77.86
REMARK 500    ASP B 117     -160.83   -167.82
REMARK 500    PHE B 149      134.13    -38.68
REMARK 500    SER B 183     -119.15     64.39
REMARK 500    PHE B 212       62.32     25.35
REMARK 500    LEU B 232      -69.86     75.21
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        RMS     TYPE
REMARK 500    ARG A 301         0.13    SIDE CHAIN
REMARK 500    ARG A 311         0.26    SIDE CHAIN
REMARK 500    ARG B 311         0.26    SIDE CHAIN
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525  M RES CSSEQI
REMARK 525    HOH A 936        DISTANCE =  5.87 ANGSTROMS
REMARK 525    HOH A 937        DISTANCE =  6.06 ANGSTROMS
REMARK 525    HOH A 938        DISTANCE =  6.10 ANGSTROMS
REMARK 525    HOH A 939        DISTANCE =  6.34 ANGSTROMS
REMARK 525    HOH A 940        DISTANCE =  6.51 ANGSTROMS
REMARK 525    HOH A 941        DISTANCE =  6.75 ANGSTROMS
REMARK 525    HOH A 942        DISTANCE =  6.79 ANGSTROMS
REMARK 525    HOH B 934        DISTANCE =  5.84 ANGSTROMS
REMARK 525    HOH B 935        DISTANCE =  6.12 ANGSTROMS
REMARK 525    HOH B 936        DISTANCE =  6.33 ANGSTROMS
REMARK 525    HOH B 937        DISTANCE =  6.89 ANGSTROMS
DBREF  9LF7 A    1   336  PDB    9LF7     9LF7             1    336
DBREF  9LF7 B    1   336  PDB    9LF7     9LF7             1    336
SEQRES   1 A  336  MET ARG LEU ALA LYS LEU PRO ALA LEU ALA TYR ARG SER
SEQRES   2 A  336  ALA LYS ASP THR GLY GLU SER MET THR ASP THR PRO TYR
SEQRES   3 A  336  ILE ARG PRO ASP MET LYS ALA PHE LEU GLU MET MET ALA
SEQRES   4 A  336  GLN VAL ASN GLY PRO LYS LEU SER GLU MET SER LEU ASP
SEQRES   5 A  336  GLU ALA ARG ALA SER TYR LEU ALA MET HIS ASN LEU ALA
SEQRES   6 A  336  ASP ARG PRO ALA ARG ALA LEU PRO VAL ILE ARG ASP LEU
SEQRES   7 A  336  SER CYS PRO GLY PRO LYS GLY GLU ILE ALA LEU ARG LEU
SEQRES   8 A  336  TYR ASP PRO ARG GLU SER ARG ALA GLY PRO THR PRO VAL
SEQRES   9 A  336  ILE THR PHE PHE HIS GLY GLY GLY PHE VAL ILE GLY ASP
SEQRES  10 A  336  LEU ASP THR HIS HIS ALA LEU CYS THR GLU ILE ALA ALA
SEQRES  11 A  336  LEU MET ASP LEU PRO LEU VAL ALA VAL HIS TYR ALA ARG
SEQRES  12 A  336  ALA PRO GLU ALA PRO PHE PRO ALA ALA ILE LEU ASP CYS
SEQRES  13 A  336  GLU ALA ALA THR ARG TRP ILE ALA SER SER PRO ALA GLU
SEQRES  14 A  336  LEU GLY LEU THR ALA SER GLY ILE ILE THR ILE GLY ASP
SEQRES  15 A  336  SER ALA GLY GLY ASN ALA THR VAL VAL VAL GLY GLN LEU
SEQRES  16 A  336  LEU ALA ALA SER PRO ALA ALA VAL PRO VAL VAL LEU GLN
SEQRES  17 A  336  VAL PRO ILE PHE PRO LEU VAL ALA ASP ALA VAL SER SER
SEQRES  18 A  336  GLU SER MET ALA ALA PHE SER GLU GLY TYR LEU LEU THR
SEQRES  19 A  336  ALA GLU THR MET ALA PHE PHE ASP ALA ALA TYR GLY ALA
SEQRES  20 A  336  ASP ARG SER ASP PRO ARG GLY PHE PRO ILE LEU GLY ARG
SEQRES  21 A  336  HIS ASP ASN ALA PRO PRO THR ILE VAL VAL THR ALA SER
SEQRES  22 A  336  LEU ASP PRO ILE ARG ASP SER GLY ARG ALA TYR ALA LYS
SEQRES  23 A  336  ALA LEU ILE ASP ALA GLY ARG ASP CYS VAL PHE LEU GLU
SEQRES  24 A  336  MET ARG GLY VAL THR HIS SER PHE THR ASN LEU ARG GLN
SEQRES  25 A  336  MET VAL PRO SER THR GLN ALA ASP LEU GLU ARG VAL ILE
SEQRES  26 A  336  ALA ALA MET GLN PHE MET LEU ALA ASN PRO ALA
SEQRES   1 B  336  MET ARG LEU ALA LYS LEU PRO ALA LEU ALA TYR ARG SER
SEQRES   2 B  336  ALA LYS ASP THR GLY GLU SER MET THR ASP THR PRO TYR
SEQRES   3 B  336  ILE ARG PRO ASP MET LYS ALA PHE LEU GLU MET MET ALA
SEQRES   4 B  336  GLN VAL ASN GLY PRO LYS LEU SER GLU MET SER LEU ASP
SEQRES   5 B  336  GLU ALA ARG ALA SER TYR LEU ALA MET HIS ASN LEU ALA
SEQRES   6 B  336  ASP ARG PRO ALA ARG ALA LEU PRO VAL ILE ARG ASP LEU
SEQRES   7 B  336  SER CYS PRO GLY PRO LYS GLY GLU ILE ALA LEU ARG LEU
SEQRES   8 B  336  TYR ASP PRO ARG GLU SER ARG ALA GLY PRO THR PRO VAL
SEQRES   9 B  336  ILE THR PHE PHE HIS GLY GLY GLY PHE VAL ILE GLY ASP
SEQRES  10 B  336  LEU ASP THR HIS HIS ALA LEU CYS THR GLU ILE ALA ALA
SEQRES  11 B  336  LEU MET ASP LEU PRO LEU VAL ALA VAL HIS TYR ALA ARG
SEQRES  12 B  336  ALA PRO GLU ALA PRO PHE PRO ALA ALA ILE LEU ASP CYS
SEQRES  13 B  336  GLU ALA ALA THR ARG TRP ILE ALA SER SER PRO ALA GLU
SEQRES  14 B  336  LEU GLY LEU THR ALA SER GLY ILE ILE THR ILE GLY ASP
SEQRES  15 B  336  SER ALA GLY GLY ASN ALA THR VAL VAL VAL GLY GLN LEU
SEQRES  16 B  336  LEU ALA ALA SER PRO ALA ALA VAL PRO VAL VAL LEU GLN
SEQRES  17 B  336  VAL PRO ILE PHE PRO LEU VAL ALA ASP ALA VAL SER SER
SEQRES  18 B  336  GLU SER MET ALA ALA PHE SER GLU GLY TYR LEU LEU THR
SEQRES  19 B  336  ALA GLU THR MET ALA PHE PHE ASP ALA ALA TYR GLY ALA
SEQRES  20 B  336  ASP ARG SER ASP PRO ARG GLY PHE PRO ILE LEU GLY ARG
SEQRES  21 B  336  HIS ASP ASN ALA PRO PRO THR ILE VAL VAL THR ALA SER
SEQRES  22 B  336  LEU ASP PRO ILE ARG ASP SER GLY ARG ALA TYR ALA LYS
SEQRES  23 B  336  ALA LEU ILE ASP ALA GLY ARG ASP CYS VAL PHE LEU GLU
SEQRES  24 B  336  MET ARG GLY VAL THR HIS SER PHE THR ASN LEU ARG GLN
SEQRES  25 B  336  MET VAL PRO SER THR GLN ALA ASP LEU GLU ARG VAL ILE
SEQRES  26 B  336  ALA ALA MET GLN PHE MET LEU ALA ASN PRO ALA
HET    MES  A 401      12
HET    MES  B 401      12
HETNAM     MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID
FORMUL   3  MES    2(C6 H13 N O4 S)
FORMUL   5  HOH   *879(H2 O)
HELIX    1 AA1 ARG A   28  ASN A   42  1                                  15
HELIX    2 AA2 LYS A   45  MET A   49  5                                   5
HELIX    3 AA3 SER A   50  ASP A   66  1                                  17
HELIX    4 AA4 HIS A  121  ASP A  133  1                                  13
HELIX    5 AA5 PRO A  150  SER A  165  1                                  16
HELIX    6 AA6 SER A  183  SER A  199  1                                  17
HELIX    7 AA7 SER A  221  SER A  228  1                                   8
HELIX    8 AA8 THR A  234  GLY A  246  1                                  13
HELIX    9 AA9 PHE A  255  GLY A  259  5                                   5
HELIX   10 AB1 ILE A  277  ALA A  291  1                                  15
HELIX   11 AB2 SER A  306  LEU A  310  5                                   5
HELIX   12 AB3 PRO A  315  ASN A  334  1                                  20
HELIX   13 AB4 ARG B   28  VAL B   41  1                                  14
HELIX   14 AB5 LYS B   45  MET B   49  5                                   5
HELIX   15 AB6 SER B   50  ASP B   66  1                                  17
HELIX   16 AB7 HIS B  121  ASP B  133  1                                  13
HELIX   17 AB8 PRO B  150  SER B  165  1                                  16
HELIX   18 AB9 SER B  183  SER B  199  1                                  17
HELIX   19 AC1 SER B  221  SER B  228  1                                   8
HELIX   20 AC2 THR B  234  GLY B  246  1                                  13
HELIX   21 AC3 PHE B  255  GLY B  259  5                                   5
HELIX   22 AC4 ILE B  277  ALA B  291  1                                  15
HELIX   23 AC5 SER B  306  LEU B  310  5                                   5
HELIX   24 AC6 PRO B  315  ASN B  334  1                                  20
SHEET    1 AA1 6 VAL A  74  GLY A  82  0
SHEET    2 AA1 6 GLY A  85  ASP A  93 -1  O  LEU A  89   N  LEU A  78
SHEET    3 AA1 6 LEU A 136  HIS A 140 -1  O  LEU A 136   N  TYR A  92
SHEET    4 AA1 6 PRO A 101  PHE A 108  1  N  ILE A 105   O  VAL A 137
SHEET    5 AA1 6 THR A 173  ASP A 182  1  O  ILE A 178   N  THR A 106
SHEET    6 AA1 6 VAL A 205  ILE A 211  1  O  ILE A 211   N  GLY A 181
SHEET    1 AA2 4 THR A 267  ALA A 272  0
SHEET    2 AA2 4 CYS A 295  MET A 300  1  O  MET A 300   N  THR A 271
SHEET    3 AA2 4 CYS B 295  MET B 300 -1  O  CYS B 295   N  PHE A 297
SHEET    4 AA2 4 THR B 267  ALA B 272  1  N  THR B 271   O  MET B 300
SHEET    1 AA3 6 VAL B  74  GLY B  82  0
SHEET    2 AA3 6 GLY B  85  ASP B  93 -1  O  LEU B  89   N  LEU B  78
SHEET    3 AA3 6 LEU B 136  HIS B 140 -1  O  LEU B 136   N  TYR B  92
SHEET    4 AA3 6 PRO B 101  PHE B 108  1  N  ILE B 105   O  VAL B 137
SHEET    5 AA3 6 THR B 173  ASP B 182  1  O  ILE B 178   N  THR B 106
SHEET    6 AA3 6 VAL B 205  ILE B 211  1  O  ILE B 211   N  GLY B 181
CISPEP   1 ALA A  144    PRO A  145          0        -1.09
CISPEP   2 PHE A  149    PRO A  150          0         4.59
CISPEP   3 ALA B  144    PRO B  145          0         2.03
CISPEP   4 PHE B  149    PRO B  150          0         1.82
CRYST1   68.657   89.603  139.592  90.00  90.00  90.00 P 21 21 21    8
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.014565  0.000000  0.000000        0.00000
SCALE2      0.000000  0.011160  0.000000        0.00000
SCALE3      0.000000  0.000000  0.007164        0.00000
TER    2330      ALA A 336
TER    4655      ALA B 336
MASTER      326    0    2   24   16    0    0    6 5556    2   24   52
END