longtext: 9rf2-pdb

content
HEADER    HYDROLASE                               04-JUN-25   9RF2
TITLE     M. TUBERCULOSIS MEETS EUROPEAN LEAD FACTORY: IDENTIFICATION AND
TITLE    2 STRUCTURAL CHARACTERIZATION OF NOVEL RV0183 INHIBITORS USING X-RAY
TITLE    3 CRYSTALLOGRAPHY: ELF5
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: MONOACYLGLYCEROL LIPASE;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: MGL;
COMPND   5 EC: 3.1.1.23;
COMPND   6 ENGINEERED: YES;
COMPND   7 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: MYCOBACTERIUM TUBERCULOSIS H37RV;
SOURCE   3 ORGANISM_TAXID: 83332;
SOURCE   4 GENE: RV0183;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008
KEYWDS    RV0183, INHIBITOR, MONOACYLGLYCEROL LIPASE, HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    L.RIEGLER-BERKET,L.GOEDL,M.OBERER,N.POLIDORI
REVDAT   1   13-AUG-25 9RF2    0
JRNL        AUTH   L.RIEGLER-BERKET,L.GOEDL,M.OBERER,N.POLIDORI
JRNL        TITL   M. TUBERCULOSIS MEETS EUROPEAN LEAD FACTORY: IDENTIFICATION
JRNL        TITL 2 AND STRUCTURAL CHARACTERIZATION OF NOVEL RV0183 INHIBITORS
JRNL        TITL 3 USING X-RAY CRYSTALLOGRAPHY
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   2
REMARK   2 RESOLUTION.    1.60 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : PHENIX (1.20.1_4487: ???)
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK   3
REMARK   3    REFINEMENT TARGET : ML
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.60
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 41.39
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.5
REMARK   3   NUMBER OF REFLECTIONS             : 40788
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.190
REMARK   3   R VALUE            (WORKING SET) : 0.190
REMARK   3   FREE R VALUE                     : 0.203
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.880
REMARK   3   FREE R VALUE TEST SET COUNT      : 1989
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE
REMARK   3     1 41.3900 -  3.8600    0.99     2978   154  0.1914 0.1706
REMARK   3     2  3.8600 -  3.0600    1.00     2825   153  0.1720 0.1888
REMARK   3     3  3.0600 -  2.6700    1.00     2802   136  0.1902 0.2167
REMARK   3     4  2.6700 -  2.4300    1.00     2772   171  0.1767 0.2056
REMARK   3     5  2.4300 -  2.2600    1.00     2776   140  0.1783 0.2185
REMARK   3     6  2.2600 -  2.1200    1.00     2769   123  0.1772 0.2135
REMARK   3     7  2.1200 -  2.0200    1.00     2761   138  0.1781 0.2270
REMARK   3     8  2.0200 -  1.9300    1.00     2743   150  0.1850 0.1959
REMARK   3     9  1.9300 -  1.8500    1.00     2781   141  0.2036 0.2394
REMARK   3    10  1.8500 -  1.7900    1.00     2772   141  0.2157 0.2642
REMARK   3    11  1.7900 -  1.7300    1.00     2720   142  0.2377 0.2422
REMARK   3    12  1.7300 -  1.6800    1.00     2716   141  0.2443 0.2854
REMARK   3    13  1.6800 -  1.6400    0.99     2758   129  0.2712 0.3003
REMARK   3    14  1.6400 -  1.6000    0.96     2627   130  0.3200 0.3457
REMARK   3
REMARK   3  BULK SOLVENT MODELLING.
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL
REMARK   3   SOLVENT RADIUS     : 1.10
REMARK   3   SHRINKAGE RADIUS   : 0.90
REMARK   3   K_SOL              : NULL
REMARK   3   B_SOL              : NULL
REMARK   3
REMARK   3  ERROR ESTIMATES.
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.191
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 22.794
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : 30.94
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 29.84
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : NULL
REMARK   3    B22 (A**2) : NULL
REMARK   3    B33 (A**2) : NULL
REMARK   3    B12 (A**2) : NULL
REMARK   3    B13 (A**2) : NULL
REMARK   3    B23 (A**2) : NULL
REMARK   3
REMARK   3  TWINNING INFORMATION.
REMARK   3   FRACTION: NULL
REMARK   3   OPERATOR: NULL
REMARK   3
REMARK   3  DEVIATIONS FROM IDEAL VALUES.
REMARK   3                 RMSD          COUNT
REMARK   3   BOND      :  0.005           2335
REMARK   3   ANGLE     :  0.858           3204
REMARK   3   CHIRALITY :  0.057            359
REMARK   3   PLANARITY :  0.009            425
REMARK   3   DIHEDRAL  : 12.909            907
REMARK   3
REMARK   3  TLS DETAILS
REMARK   3   NUMBER OF TLS GROUPS  : NULL
REMARK   3
REMARK   3  NCS DETAILS
REMARK   3   NUMBER OF NCS GROUPS : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 9RF2 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 04-JUN-25.
REMARK 100 THE DEPOSITION ID IS D_1292148152.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 22-NOV-22
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 7.5
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : PETRA III, DESY
REMARK 200  BEAMLINE                       : P11
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.033230
REMARK 200  MONOCHROMATOR                  : NULL
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : PIXEL
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS EIGER2 X 16M
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 40806
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.600
REMARK 200  RESOLUTION RANGE LOW       (A) : 41.390
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.5
REMARK 200  DATA REDUNDANCY                : 2.000
REMARK 200  R MERGE                    (I) : NULL
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 17.5400
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.60
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.66
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : NULL
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : NULL
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: PHASER
REMARK 200 STARTING MODEL: NULL
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 45.49
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.26
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: PEG 1000, PEG 3350, MPD, HEPES, MOPS,
REMARK 280  CHAPS, CHAPSO, SODIUM GLYCOCHOLATE HYDRATE, TAUROCHOLIC ACID
REMARK 280  SODIUM SALT HYDRATE, PH 7.5, VAPOR DIFFUSION, SITTING DROP,
REMARK 280  TEMPERATURE 293.15K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 2 21 21
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   X,-Y,-Z
REMARK 290       3555   -X,Y+1/2,-Z+1/2
REMARK 290       4555   -X,-Y+1/2,Z+1/2
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       41.39200
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       45.35200
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       41.39200
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       45.35200
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 130 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 10670 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -9.0 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     MET A   -27
REMARK 465     SER A   -26
REMARK 465     TYR A   -25
REMARK 465     TYR A   -24
REMARK 465     HIS A   -23
REMARK 465     HIS A   -22
REMARK 465     HIS A   -21
REMARK 465     HIS A   -20
REMARK 465     HIS A   -19
REMARK 465     HIS A   -18
REMARK 465     ASP A   -17
REMARK 465     TYR A   -16
REMARK 465     ASP A   -15
REMARK 465     ILE A   -14
REMARK 465     PRO A   -13
REMARK 465     THR A   -12
REMARK 465     THR A   -11
REMARK 465     GLU A   -10
REMARK 465     ASN A    -9
REMARK 465     LEU A    -8
REMARK 465     TYR A    -7
REMARK 465     PHE A    -6
REMARK 465     GLN A    -5
REMARK 465     GLY A    -4
REMARK 465     ALA A    -3
REMARK 465     MET A    -2
REMARK 465     GLY A    -1
REMARK 465     SER A     0
REMARK 465     MET A     1
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE
REMARK 500   O    PRO A   124     O    HOH A   401              2.17
REMARK 500   NH1  ARG A    52     OD1  ASP A   269              2.19
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    ASP A  26       40.27    -89.15
REMARK 500    ASP A  26       41.88    -89.15
REMARK 500    GLU A  41     -150.69   -103.51
REMARK 500    SER A 110     -122.10     62.50
REMARK 500    ALA A 134       59.70     37.07
REMARK 500    ALA A 138       47.84   -140.84
REMARK 500
REMARK 500 REMARK: NULL
DBREF  9RF2 A    1   279  UNP    O07427   MGLL_MYCTU       1    279
SEQADV 9RF2 MET A  -27  UNP  O07427              INITIATING METHIONINE
SEQADV 9RF2 SER A  -26  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 TYR A  -25  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 TYR A  -24  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 HIS A  -23  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 HIS A  -22  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 HIS A  -21  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 HIS A  -20  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 HIS A  -19  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 HIS A  -18  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 ASP A  -17  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 TYR A  -16  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 ASP A  -15  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 ILE A  -14  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 PRO A  -13  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 THR A  -12  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 THR A  -11  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 GLU A  -10  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 ASN A   -9  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 LEU A   -8  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 TYR A   -7  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 PHE A   -6  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 GLN A   -5  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 GLY A   -4  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 ALA A   -3  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 MET A   -2  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 GLY A   -1  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 SER A    0  UNP  O07427              EXPRESSION TAG
SEQADV 9RF2 ALA A   74  UNP  O07427    LYS    74 ENGINEERED MUTATION
SEQRES   1 A  307  MET SER TYR TYR HIS HIS HIS HIS HIS HIS ASP TYR ASP
SEQRES   2 A  307  ILE PRO THR THR GLU ASN LEU TYR PHE GLN GLY ALA MET
SEQRES   3 A  307  GLY SER MET THR THR THR ARG THR GLU ARG ASN PHE ALA
SEQRES   4 A  307  GLY ILE GLY ASP VAL ARG ILE VAL TYR ASP VAL TRP THR
SEQRES   5 A  307  PRO ASP THR ALA PRO GLN ALA VAL VAL VAL LEU ALA HIS
SEQRES   6 A  307  GLY LEU GLY GLU HIS ALA ARG ARG TYR ASP HIS VAL ALA
SEQRES   7 A  307  GLN ARG LEU GLY ALA ALA GLY LEU VAL THR TYR ALA LEU
SEQRES   8 A  307  ASP HIS ARG GLY HIS GLY ARG SER GLY GLY ALA ARG VAL
SEQRES   9 A  307  LEU VAL ARG ASP ILE SER GLU TYR THR ALA ASP PHE ASP
SEQRES  10 A  307  THR LEU VAL GLY ILE ALA THR ARG GLU TYR PRO GLY CYS
SEQRES  11 A  307  LYS ARG ILE VAL LEU GLY HIS SER MET GLY GLY GLY ILE
SEQRES  12 A  307  VAL PHE ALA TYR GLY VAL GLU ARG PRO ASP ASN TYR ASP
SEQRES  13 A  307  LEU MET VAL LEU SER ALA PRO ALA VAL ALA ALA GLN ASP
SEQRES  14 A  307  LEU VAL SER PRO VAL VAL ALA VAL ALA ALA LYS LEU LEU
SEQRES  15 A  307  GLY VAL VAL VAL PRO GLY LEU PRO VAL GLN GLU LEU ASP
SEQRES  16 A  307  PHE THR ALA ILE SER ARG ASP PRO GLU VAL VAL GLN ALA
SEQRES  17 A  307  TYR ASN THR ASP PRO LEU VAL HIS HIS GLY ARG VAL PRO
SEQRES  18 A  307  ALA GLY ILE GLY ARG ALA LEU LEU GLN VAL GLY GLU THR
SEQRES  19 A  307  MET PRO ARG ARG ALA PRO ALA LEU THR ALA PRO LEU LEU
SEQRES  20 A  307  VAL LEU HIS GLY THR ASP ASP ARG LEU ILE PRO ILE GLU
SEQRES  21 A  307  GLY SER ARG ARG LEU VAL GLU CYS VAL GLY SER ALA ASP
SEQRES  22 A  307  VAL GLN LEU LYS GLU TYR PRO GLY LEU TYR HIS GLU VAL
SEQRES  23 A  307  PHE ASN GLU PRO GLU ARG ASN GLN VAL LEU ASP ASP VAL
SEQRES  24 A  307  VAL ALA TRP LEU THR GLU ARG LEU
HET    8KE  A 301      28
HET    I3F  A 302      56
HET     CL  A 303       1
HETNAM     8KE [1-[6-[(4-METHOXYPHENYL)AMINO]PYRIMIDIN-4-YL]PYRAZOL-4-
HETNAM   2 8KE  YL]-[(3S)-3-OXIDANYLPYRROLIDIN-1-YL]METHANONE
HETNAM     I3F [1-[6-[(4-METHOXYPHENYL)AMINO]PYRIMIDIN-4-YL]PYRAZOL-4-
HETNAM   2 I3F  YL]-[(3R)-3-OXIDANYLPYRROLIDIN-1-YL]METHANONE
HETNAM      CL CHLORIDE ION
FORMUL   2  8KE    C19 H20 N6 O3
FORMUL   3  I3F    C19 H20 N6 O3
FORMUL   4   CL    CL 1-
FORMUL   5  HOH   *62(H2 O)
HELIX    1 AA1 ILE A   13  ASP A   15  5                                   3
HELIX    2 AA2 HIS A   42  ARG A   45  5                                   4
HELIX    3 AA3 TYR A   46  ALA A   56  1                                  11
HELIX    4 AA4 ASP A   80  TYR A   99  1                                  20
HELIX    5 AA5 SER A  110  ARG A  123  1                                  14
HELIX    6 AA6 ALA A  138  LEU A  142  5                                   5
HELIX    7 AA7 SER A  144  GLY A  155  1                                  12
HELIX    8 AA8 ASP A  167  ILE A  171  5                                   5
HELIX    9 AA9 ASP A  174  THR A  183  1                                  10
HELIX   10 AB1 ALA A  194  ALA A  211  1                                  18
HELIX   11 AB2 PRO A  212  LEU A  214  5                                   3
HELIX   12 AB3 ILE A  231  VAL A  241  1                                  11
HELIX   13 AB4 GLU A  257  GLU A  261  5                                   5
HELIX   14 AB5 GLU A  263  LEU A  279  1                                  17
SHEET    1 AA1 8 THR A   3  ALA A  11  0
SHEET    2 AA1 8 ARG A  17  PRO A  25 -1  O  ILE A  18   N  PHE A  10
SHEET    3 AA1 8 LEU A  58  LEU A  63 -1  O  ALA A  62   N  ASP A  21
SHEET    4 AA1 8 ALA A  31  ALA A  36  1  N  VAL A  33   O  VAL A  59
SHEET    5 AA1 8 LYS A 103  HIS A 109  1  O  ILE A 105   N  VAL A  32
SHEET    6 AA1 8 LEU A 129  SER A 133  1  O  SER A 133   N  GLY A 108
SHEET    7 AA1 8 LEU A 218  GLY A 223  1  O  LEU A 219   N  LEU A 132
SHEET    8 AA1 8 VAL A 246  TYR A 251  1  O  LYS A 249   N  VAL A 220
SHEET    1 AA2 2 PRO A 162  GLN A 164  0
SHEET    2 AA2 2 VAL A 192  PRO A 193 -1  O  VAL A 192   N  GLN A 164
CRYST1   40.411   82.784   90.704  90.00  90.00  90.00 P 2 21 21     4
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.024746  0.000000  0.000000        0.00000
SCALE2      0.000000  0.012080  0.000000        0.00000
SCALE3      0.000000  0.000000  0.011025        0.00000
TER    2190      LEU A 279
MASTER      277    0    3   14   10    0    0    6 2241    1   84   24
END